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Microsatellite analysis of pacu broodstocks used in the stocking program of Paranapanema River, Brazil
Provedor de dados: 63
Autores: Povh,Jayme Aparecido; Ribeiro,Ricardo Pereira; Lopera-Barrero,Nelson Mauricio; Jacometo,Carolina Bespalhok; Vargas,Lauro; Gomes,Patrícia Cristina; Lopes,Taís da Silva.
Monitoring the genetic diversity has fundamental importance for fish stocking programs. This experiment aims to evaluate the genetic diversity in two hatchery stations (A and B) with pacu Piaractus mesopotamicus (Holmberg, 1887) in Andirá, state of Paraná, Brazil used in stocking programs of Paranapanema River. Six microsatellite loci were amplified using DNA extracted from 60 fin-clipping samples. The broodstock B had the average number of alleles and the mean heterozygosity (alleles: 3.7 and H O: 0.628) higher than the broodstock A (alleles: 3.5 and H O: 0.600). Alleles with low frequency levels were observed in the both broodstocks. The positive coefficients of endogamy in the locus Pme2 (broodstock A: F IS = 0.30 and broodstock B: F IS = 0.20), Pme5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piaractus mesopotamicus; Genetic variability; Hatchery stations; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300006