Consulta: subjectFacets:"Hepatocytes"
Registros recuperados: 10
Data/hora: 10/06/2024 14:27:20

Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus
Provedor de dados: 74
Autores: Almeida,Terezinha M.B. de; Leitão,Regina Maria C.; Carrilho,Flair J.; Sonohara,Shigueko.
Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fastgreen staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronuclei; Hepatocytes; Chronic hepatitis C; Fibrosis progression; HCV-patients.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300003

Azaspiracids Increase Mitochondrial Dehydrogenases Activity in Hepatocytes: Involvement of Potassium and Chloride Ions
Provedor de dados: 5
Autores: Pelin, Marco; Kilcoyne, Jane; Florio, Chiara; Hess, Philipp; Tubaro, Aurelia; Sosa, Silvio.
Background: Azaspiracids (AZAs) are marine toxins that are produced by Azadinium and Amphidoma dinoflagellates that can contaminate edible shellfish inducing a foodborne poisoning in humans, which is characterized by gastrointestinal symptoms. Among these, AZA1, -2, and -3 are regulated in the European Union, being the most important in terms of occurrence and toxicity. In vivo studies in mice showed that, in addition to gastrointestinal effects, AZA1 induces liver alterations that are visible as a swollen organ, with the presence of hepatocellular fat droplets and vacuoles. Hence, an in vitro study was carried out to investigate the effects of AZA1, -2, and -3 on liver cells, using human non-tumor IHH hepatocytes. Results: The exposure of IHH cells to...
Tipo: Text
Palavras-chave: Azaspiracids; Hepatocytes; Mitochondrial activity; Mechanism of toxicity.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00494/60526/63989.pdf

Can the Intermittent Training Generate Alterations on the Liver Tissue of Rats Submitted to a Hyperlipidic Diet?
Provedor de dados: 77
Autores: Magalhães,Alan José Barbosa; Castoldi,Robson Chacon; Camargo,Regina Celi Trindade; Ozaki,Guilherme Akio Tamura; Costalonga,Regiane Rocha; Moreira,Rafael Junges; Seraphim,Patricia Monteiro; Camargo Filho,José Carlos Silva.
Studies indicate that increasing physical activity and decreasing levels of fat in the liver help to decrease the risk of morbidity from liver conditions. This study aimed to evaluate the effects of an eight week intermittent training program on the liver tissue of rats subjected to a hyperlipidic diet. The study consisted of 30 male Wistar rats, divided into the following groups: Sedentary Control (SC) Exercise Control (EC) (fed on standard feed and water) Sedentary Obese (SO) and Exercise Obese (EO) (fed on bacon, ham, sausage, biscuits, soda and standard feed), which performed intermittent training through electrically stimulated jumps, with three sets of 12 repetitions, three times per week for eight weeks. At the end of the training period, the...
Tipo: Journal article
Palavras-chave: High fat diet; Exercise; Hepatocytes.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100014

Morphology and Histochemistry of the Liver of Carnivorous Fish Hemisorubim platyrhynchos
Provedor de dados: 77
Autores: Faccioli,Claudemir Kuhn; Chedid,Renata Alari; Bombonato,Maria Terezinha Siqueira; Vicentini,Carlos Alberto; Vicentini,Irene Bastos Franceschini.
The aim of this study was to characterize the normal structure of the liver of Hemisorubim platyrhynchos, a carnivorous freshwater catfish found in Neotropical region, using gross anatomy, histology and histochemistry. Anatomically, the liver presents C-shaped and only two lobes: smaller right and bigger left. The gallbladder is located in right lobe and shows elongated shaped. Histological analysis demonstrated that the hepatic parenchyma is made of two hepatocytes plates surrounded by sinusoids. The hepatocytes are polygonal-shaped cells, with spherical nucleus and a dark prominent nucleolus. The cytoplasm presents large amount of lipids and glycogen deposits PAS positives. There are no hepatic lobules or portal triads. Bile ducts are lined by columnar...
Tipo: Journal article
Palavras-chave: Anatomy; Histology; Hepatocytes; Extrahepatic Pancreas; Teleost.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200055

Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat
Provedor de dados: 77
Autores: Al-Motabagani,Mohamed Akram.
Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell...
Tipo: Journal article
Palavras-chave: Methotrexate; Hepatocytes; Hepatic lobules; Lycogen; Enzyme activity.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400020

Comparative histology in the liver and spleen of three species of freshwater teleost
Provedor de dados: 83
Autores: Sales,Camila F.; Silva,Regianne F.; Amaral,Marília G. C.; Domingos,Fabrício F. T.; Ribeiro,Rosy I. M. A.; Thomé,Ralph G.; Santos,Hélio B.
ABSTRACT We describe and compare the histology of liver and spleen ofGeophagus brasiliensis (Perciformes), Hypostomus francisci (Siluriformes) and Hoplias aff. malabaricus (Characiformes), tropical freshwater fishes. InG. brasiliensisandH. aff. malabaricusthe hepatocytes were arranged in tubular form whereas in H. franciscithey cord-like. In all species, hepatocytes presented glycogen, but in G. brasiliensis and H. aff. malabaricus they showed strong stained for hemossiderin in the cytoplasm. InG. brasiliensis and H. aff. malabaricus, melanomacrophage centres (MMCs) were associated to hepatic structures and only in G. brasiliensis was observed intrahepatic exocrine pancreas. The spleen, in all species, was characterized by red and white pulp without...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extracellular matrix; Hepatocytes; Intrahepatic exocrine pancreas; Melanomacrophage centres; Red and White pulp.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100209

Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies
Provedor de dados: 47
Autores: Lopez,Lidia M.; Dubin,Marta; Carrizo,Patricia H.; Burgos,Mario H.; Pellegrino de Iraldi,Amanda; Stoppani,Andrés O. M.
CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho&amp;#091;1,2b&amp;#093;pyran- 5,6-dione; CG-NQ), a b-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoptosis; O-naphthoquinones; Hepatocytes; Chromatin Alteration; Mitochondrial Swelling Membrane; Blebs.
Ano: 2003
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000200007

Hepatocytes, rather than leukocytes reverse DNA damage in vivo induced by whole body y-irradiation of mice, as shown by the alkaline comet assay
Provedor de dados: 48
Autores: PINCHEIRA,JUANA; CARRERA,PILAR; MARCELAIN,KATHERINE; DE LA TORRE,CONSUELO.
DNA damage repair was assessed in quiescent (G0) leukocytes and in hepatocytes of mice, after 1 and 2 hours recovery from a single whole body y-irradiation with 0.5, 1 or 2 Gy. Evaluation of single-strand breaks (SSB) and alkali-labile sites together were carried out by a single-cell electrophoresis at pH&gt;13.0 (alkaline comet assay). In non-irradiated (control) mice, the constitutive, endogenous DNA damage (basal) was around 1.5 times higher in leukocytes than in hepatocytes. Irradiation immediately increased SSB frequency in both cell types, in a dose-dependent manner. Two sequential phases took place during the in vivo repair of the radio-induced DNA lesions. The earliest one, present in both hepatocytes and leukocytes, further increased the SSB...
Tipo: Journal article
Palavras-chave: Alkaline comet assay; Ionizing radiation; Hepatocytes; Leukocytes; In vivo DNA repair.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200011

The Risk Evaluation of Tungsten Oxide Nanoparticles in Cultured Rat Liver Cells for Its Safe Applications in Nanotechnology
Provedor de dados: 52
Autores: Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven.
Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (&lt;100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p&lt;0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532

The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone
Provedor de dados: 56
Autores: Ma,Huiwen; Wang,Ping; Jin,Dan; Jia,Ting; Mao,Hong; Zhang,Jiandi; Zhao,Shi.
Hormones regulate hepatic gene expressions to maintain metabolic homeostasis. Ectonucleotide pyrophosphatase/phosphodiesterase 1 has been thought to interfere with insulin signaling. To determine its potential role in the regulation of metabolism, we analyzed its gene (Enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma HepG2 cells treated with insulin and dexamethasone using northern blot and real-time PCR techniques. Hepatic Enpp1 expression was induced by fasting and reduced by refeeding in the rat liver. In primary rat hepatocytes and HepG2 hepatoma cells, insulin reduced Enpp1 mRNA abundance, whereas dexamethasone induced it. Dexamethasone disrupted the insulin-reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ectonucleotide pyrophosphatase/phosphodiesterase 1; Insulin; Dexamethasone; Hepatocytes; Regulation of gene expression.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400609