Consulta: subjectFacets:"Herbaspirillum seropedicae"
Registros recuperados: 14
Data/hora: 11/06/2024 07:06:37

Expression, purification and DNA-binding activities of two putative ModE proteins of Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Provedor de dados: 74
Autores: Souza,André L.F.; Chubatsu,Leda S.; Souza,Emanuel M.; Pedrosa,Fábio O.; Monteiro,Rose A.; Rego,Fabiane G.M.; Rigo,Liu U.
In prokaryotes molybdenum is taken up by a high-affinity ABC-type transporter system encoded by the modABC genes. The endophyte β-Proteobacterium Herbaspirillum seropedicae has two modABC gene clusters and two genes encoding putative Mo-dependent regulator proteins (ModE1 and ModE2). Analysis of the amino acid sequence of the ModE1 protein of H. seropedicae revealed the presence of an N-terminal domain containing a DNA-binding helix-turn-helix motif (HTH) and a C-terminal domain with a molybdate-binding motif. The second putative regulator protein, ModE2, contains only the helix-turn-helix motif, similar to that observed in some sequenced genomes. We cloned the modE1 (810 bp) and modE2 (372 bp) genes and expressed them in Escherichia coli as His-tagged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; ModE1 protein; ModE2 protein.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400022

Early colonization pattern of maize (Zea mays L. Poales, Poaceae) roots by Herbaspirillum seropedicae (Burkholderiales, Oxalobacteraceae)
Provedor de dados: 74
Autores: Monteiro,Rose A.; Schmidt,Maria A.; Baura,Valter A. de; Balsanelli,Eduardo; Wassem,Roseli; Yates,Marshall G.; Randi,Marco A.F.; Pedrosa,Fábio O.; Souza,Emanuel M. de.
The bacterium Herbaspirillum seropedicae is an endophytic diazotroph found in several plants, including economically important poaceous species. However, the mechanisms involved in the interaction between H. seropedicae and these plants are not completely characterized. We investigated the attachment of Herbaspirillum to maize roots and the invasion of the roots by this bacterium using H. seropedicae strain SMR1 transformed with the suicide plasmid pUTKandsRed, which carries a mini-Tn5 transposon containing the gene for the Discosoma red fluorescent protein (Dsred) constitutively expressed together with the kanamycin resistance gene. Integration of the mini-Tn5 into the bacterial chromosome yielded the mutant H. seropedicae strain RAM4 which was capable of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Confocal microscopy; Diazotroph; Endophytic; Herbaspirillum seropedicae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500021

Turfa como veículo para inoculação de bactérias diazotróficas na cultura de arroz.
Provedor de dados: 14
Autores: FERREIRA, J. S.; GUIMARÃES, S. L.; SABINO, D. C. C.; BALDANI, J. I.; BALDANI, V. L. D.
bitstream/CNPAB-2010/27179/1/cot049.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Bactéria diazotrófica endofítica; Inoculation; Endophytic diazotrophy bacteria.; Arroz; Inoculação; Recurso Energético; Turfa.; Burkholderia kururiensis; Herbaspirillum seropedicae; Energy resources; Peat; Rice.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/624198

Recobrimento de sementes de milho com ácidos húmicos e bactérias diazotróficas endofíticas
Provedor de dados: 86
Autores: Conceição,Patrícia Marluci da; Vieira,Henrique Duarte; Canellas,Luciano Pasqualoto; Marques Júnior,Roberto Batista; Olivares,Fábio Lopes.
O objetivo deste trabalho foi avaliar o efeito do recobrimento de sementes de milho com ácidos húmicos (AH), bactérias diazotróficas endofíticas e o uso em conjunto de AH e bactérias diazotróficas endofíticas, na estimulação do crescimento vegetal e na população de bactérias estabelecidas na planta hospedeira. A adição de AH, bactérias e o uso em conjunto estimularam o crescimento vegetal. Os AH utilizados no recobrimento de sementes de milho têm menor capacidade de estimular o crescimento radicular, em comparação ao uso em solução. O recobrimento de sementes é uma opção de inoculação de bactérias diazotróficas endofíticas da espécie Herbaspirillum seropedicae (Z67).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; Zea mays; Bioestimulantes; Calcário.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000400015

Adição de molibdênio ao inoculante turfoso com bactérias diazotróficas usado em duas cultivares de arroz irrigado
Provedor de dados: 86
Autores: Guimarães,Salomão Lima; Baldani,José Ivo; Baldani,Vera Lúcia Divan; Jacob-Neto,Jorge.
O objetivo deste trabalho foi avaliar o efeito da utilização de bactérias diazotróficas com ou sem molibdênio em duas cultivares de arroz com diferentes potenciais de fixação biológica de nitrogênio. Dois experimentos foram montados: o primeiro foi conduzido em condições gnotobióticas, com utilização das estirpes BR 11417 (ZAE94 - Herbaspirillum seropedicae) e BR 11340 (M130 - Burkholderia sp.), duas doses de molibdênio, 1,12 e 2,25 g, e duas fontes, molibdato de sódio e molibdato de amônio. As sementes foram peletizadas com inoculante turfoso com bactérias diazotróficas e o molibdênio. Em seguida as cultivares foram plantadas em tubos de ensaio com capacidade para 120 mL com 60 mL da solução de Hoagland sem nitrogênio por 30 dias. Foi avaliada a matéria...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oriza sativa; Herbaspirillum seropedicae; Burkholderia; Molibdato de sódio; Molibdato de amônio.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000300013

Promoção de enraizamento de microtoletes de cana-de-açúcar pelo uso conjunto de substâncias húmicas e bactérias diazotróficas endofíticas
Provedor de dados: 90
Autores: Marques Júnior,Roberto Batista; Canellas,Luciano Pasqualoto; Silva,Lúcia Gracinda da; Olivares,Fábio Lopes.
Além do fornecimento direto de nutrientes por meio da mineralização da matéria orgânica ou pela fixação biológica de N2, as substâncias húmicas e as bactérias diazotróficas endofíticas podem afetar diretamente o metabolismo vegetal, modificando o padrão de crescimento e desenvolvimento das plantas. O objetivo deste trabalho foi avaliar o efeito do uso em conjunto de ácidos húmicos (AH) e Herbaspirilum seropedicae, uma bactéria endofítica fixadora de N2, sobre o crescimento radicular de microtoletes de cana-de-açúcar do cultivar RB 72 454 tratados termicamente ou não. Após o tratamento térmico, os microtoletes foram imersos por 12 h em água (controle), numa solução que continha 20 mg L-1 de C de AH isolados de vermicomposto, H. seropedicae estirpe HRC 54 na...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; Termoterapia; Ácidos húmicos.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832008000300020

Physiological quality of corn seeds reinoculated with diazotrophic bacteria
Provedor de dados: 22
Autores: Sousa, Ricardo Felipe Braga; Guimarães, Vandeir Francisco; Pinto Jr., Artur Soares; Costa, Andréia Cristina Peres Rodrigues; Offemann, Luiz Claudio; Fuchs, Felipe; Coelho, Gustavo Ferreira.
This work aimed to evaluate physiological characteristics of corn seeds provenient from cultivation associated with Azospirillum brasilense and Herbaspirillum seropedicae and reinoculated with Azospirillum brasilense. The seeds were coming from cultivation in field conditions, and the treatments were: T1 - control; T2 - 60 kg hectare-1 N, T3 - inoculation (Azospirillum brasilense - AbV5); T4 - inoculation (Azospirillum brasilense) + 60 kg hectare-1 N; T5 - inoculation (Herbaspirillum seropedicae - SMR1) and T6 - inoculation (Herbaspirillum seropedicae) + 60 kg hectare-1 N. The treatments were divided in two separate blocks, being one reinoculated with A. brasilense (AbV5). The experimental design was completely randomized, in factorial scheme (6x2), being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Azospirillum brasilense; Herbaspirillum seropedicae; Seeds physiology; Reinoculation; Zea mays.
Ano: 2015
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/8753

Seleção de inoculantes à base de turfa contendo bactérias diazotróficas em duas variedades de arroz - DOI: 10.4025/actasciagron.v32i1.732
Provedor de dados: 1
Autores: Ferreira, Joilson Silva; EMBRAPA; Baldani, José Ivo; EMBRAPA; Baldani, Vera Lúcia Divan; EMBRAPA.
O estudo consistiu em avaliar a sobrevivência e a eficiência das bactérias diazotróficas Herbaspirillum seropedicae, Burkholderia sp. e Azospirillum brasilense, inoculadas em dois tipos de turfas. A sobrevivência das bactérias foi determinada pelo método do Número Mais Provável (NMP), durante o armazenamento de até seis meses. A eficiência foi avaliada pela contribuição destas bactérias nos parâmetros agronômicos de acúmulo de massa seca e N-total dos grãos nas variedades de arroz IR42 e IAC4440. O experimento foi conduzido em vasos com solo, em delineamento inteiramente casualizado com três repetições, em arranjo fatorial 2 x 4. Os dados obtidos mostraram que o inoculante contendo a estirpe de Burkholderia sp. manteve o número de células viáveis em torno...
Palavras-chave: 5.01.00.00-9    Agronomia fixação biológica nitrogênio; Veículos de inoculação; Herbaspirillum seropedicae Agronomia nitrogen biological fixation; Inoculant carriers; Herbaspirillum seropedicae.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/732

A simple and efficient method for poly-3-hydroxybutyrate quantification in diazotrophic bacteria within 5 minutes using flow cytometry
Provedor de dados: 56
Autores: Alves,L.P.S.; Almeida,A.T.; Cruz,L.M.; Pedrosa,F.O.; de Souza,E.M.; Chubatsu,L.S.; Müller-Santos,M.; Valdameri,G.
The conventional method for quantification of polyhydroxyalkanoates based on whole-cell methanolysis and gas chromatography (GC) is laborious and time-consuming. In this work, a method based on flow cytometry of Nile red stained bacterial cells was established to quantify poly-3-hydroxybutyrate (PHB) production by the diazotrophic and plant-associated bacteria, Herbaspirillum seropedicae and Azospirillum brasilense. The method consists of three steps: i) cell permeabilization, ii) Nile red staining, and iii) analysis by flow cytometry. The method was optimized step-by-step and can be carried out in less than 5 min. The final results indicated a high correlation coefficient (R2=0.99) compared to a standard method based on methanolysis and GC. This method...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flow cytometry; Nile red; Poly-3-hydroxybutyrate; Herbaspirillum seropedicae; Azospirillum brasilense.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100606

The RecX protein interacts with the RecA protein and modulates its activity in Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Galvão,C.W.; Souza,E.M.; Etto,R.M.; Pedrosa,F.O.; Chubatsu,L.S.; Yates,M.G.; Schumacher,J.; Buck,M.; Steffens,M.B.R.
DNA repair is crucial to the survival of all organisms. The bacterial RecA protein is a central component in the SOS response and in recombinational and SOS DNA repairs. The RecX protein has been characterized as a negative modulator of RecA activity in many bacteria. The recA and recX genes of Herbaspirillum seropedicae constitute a single operon, and evidence suggests that RecX participates in SOS repair. In the present study, we show that the H. seropedicae RecX protein (RecX Hs) can interact with the H. seropedicaeRecA protein (RecA Hs) and that RecA Hs possesses ATP binding, ATP hydrolyzing and DNA strand exchange activities. RecX Hs inhibited 90% of the RecA Hs DNA strand exchange activity even when present in a 50-fold lower molar concentration than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RecA; RecX; Herbaspirillum seropedicae; SOS repair.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200004

Evidence for the endophytic colonization of Phaseolus vulgaris(common bean) roots by the diazotroph Herbaspirillum seropedicae
Provedor de dados: 56
Autores: Schmidt,M.A.; Souza,E.M.; Baura,V.; Wassem,R.; Yates,M.G.; Pedrosa,F.O.; Monteiro,R.A.
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium, which associates with important agricultural plants. In the present study, we have investigated the attachment to and internal colonization of Phaseolus vulgaris roots by the H. seropedicae wild-type strain SMR1 and by a strain of H. seropedicae expressing a red fluorescent protein (DsRed) to track the bacterium in the plant tissues. Two-day-old P. vulgaris roots were incubated at 30°C for 15 min with 6 x 10(8) CFU/mL H. seropedicae SMR1 or RAM4. Three days after inoculation, 4 x 10(4) cells of endophytic H. seropedicae SMR1 were recovered per gram of fresh root, and 9 days after inoculation the number of endophytes increased to 4 x 10(6) CFU/g. The identity of the recovered bacteria was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Herbaspirillum seropedicae; Phaseolus vulgaris; Confocal microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300001

Effect of point mutations on Herbaspirillum seropedicae NifA activity
Provedor de dados: 56
Autores: Aquino,B.; Stefanello,A.A.; Oliveira,M.A.S.; Pedrosa,F.O.; Souza,E.M.; Monteiro,R.A.; Chubatsu,L.S.
NifA is the transcriptional activator of the nif genes in Proteobacteria. It is usually regulated by nitrogen and oxygen, allowing biological nitrogen fixation to occur under appropriate conditions. NifA proteins have a typical three-domain structure, including a regulatory N-terminal GAF domain, which is involved in control by fixed nitrogen and not strictly required for activity, a catalytic AAA+ central domain, which catalyzes open complex formation, and a C-terminal domain involved in DNA-binding. In Herbaspirillum seropedicae, a β-proteobacterium capable of colonizing Graminae of agricultural importance, NifA regulation by ammonium involves its N-terminal GAF domain and the signal transduction protein GlnK. When the GAF domain is removed, the protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological nitrogen fixation; Herbaspirillum seropedicae; NifA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800683

Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis
Provedor de dados: 58
Autores: Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001

Evaluation of the biological nitrogen fixation contribution in sugarcane plants originated from seeds and inoculated with nitrogen-fixing endophytes
Provedor de dados: 58
Autores: Canuto,Erineudo de Lima; Oliveira,André Luis Martinez de; Reis,Verônica Massena; Baldani,José Ivo.
The inoculation technique with endophytic diazotrophic bacteria in sugarcane has been shown as an alternative practice to plant growth promotion. The aim of this work was to evaluate the biological nitrogen fixation (BNF) contribution by different strains of Herbaspirillum seropedicae and Gluconacetobacter diazotrophicus in sugarcane plant inoculated from seeds. The seeds were planted in pots filled with non-sterile soil, inoculated with the bacterial strains and grown 10 months outside of the greenhouse. The BNF contribution of the inoculated bacteria varied depending on the plant species used as a control. The highest BNF contribution as well as the highest populations of reisolated bacteria was observed with inoculation of H. seropedicae strains. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endophytic diazotrophic bacteria; Sugarcane; Biological nitrogen fixation; Herbaspirillum seropedicae; Gluconacetobacter diazotrophicus.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500021