Consulta: subjectFacets:"Heterologous primers"
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Cross-amplification of heterologous microsatellite markers in Piracanjuba
Provedor de dados: 65
Autores: Castro,Pedro Luiz de; Ribeiro,Ricardo Pereira; Santos,Silvio Carlos Alves dos; Goes,Elenice Souza dos Reis; Souza,Felipe Pinheiro de; Poveda-Parra,Angela Rocio; Vargas,Lauro; Urrea-Rojas,Angela Maria; Lopera-Barrero,Nelson Mauricio.
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brycon orbignyanus; Conservation; Molecular markers; Heterologous primers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402