Consulta: subjectFacets:"Immunoassays"
Registros recuperados: 3
Data/hora: 11/06/2024 03:30:25

Análisis de los niveles de vitelogenina séricas en individuos neomachos de trucha arcoiris Oncorhynchus mykis (Smith y Stearly, 1989)
Provedor de dados: 20
Autores: Guzmán Riffo, C.N.
Debido a la importancia que han adquirido los especímenes neomachos en la producción de poblaciones todo-hembras triploides, se ha hecho cada vez mas necesario investigar la manera de poder reconocer su condición sexual sin la necesidad de sacrificar al pez. Este conocimiento permitiría entregar al acuicultor una herramienta necesaria para el manejo de éstos stoks. En este estudio se analiza la factibilidad de reconocer a un neomacho de trucha arcoiris (Oncorhynchus mykiss), por medio de un análisis serológico, basándose en que un neomacho por ser un individuo hembra revertido hormonalmente de sexo, presentaría niveles detectables de la proteína femenina vitelogenina. Se compararon las concentraciones de proteínas séricas presentes en machos, hembras,...
Tipo: Theses and Dissertations
Palavras-chave: Vitellogenesis; Serological studies; Immunoassays.
Ano: 2003
URL: http://hdl.handle.net/1834/4556

Conventional serological performance in diagnosis of Chagas' disease in southern Brazil
Provedor de dados: 55
Autores: Araújo,Anelise Bergmann; Berne,Maria Elisabeth Aires.
Results of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method. From 161 samples, 65.84% were positive in all tests, while 34.16% presents mismatch result in at least one of the tests. All techniques tested presented false-positive and/or false-negative results as follows: Enzyme-linked immunosorbent assay 1 had more false-positive results (lower specificity), indirect immunofluorescence had the highest rate of false-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosoma cruzi; Chagas' disease; Serodiagnosis; Immunoassays.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200010

Detection of specific IgE antibodies in parasite diseases
Provedor de dados: 56
Autores: Souza-Atta,M.L.B.; Araújo,M.I.; D'Oliveira Júnior,A.; Ribeiro-de-Jesus,A.; Almeida,R.P.; Atta,A.M.; Carvalho,E.M.
Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: IgE antibodies; Immunoassays; Schistosomiasis mansoni; Visceral leishmaniasis; Kala-azar.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900008