Consulta: subjectFacets:"Immunodetection"
Registros recuperados: 3
Data/hora: 09/06/2024 09:32:20

Aislamiento y caracterización bioquímica de metabolitos producidos por rizobacterias que solubilizan fosfato.
Provedor de dados: 32
Autores: Paredes Mendoza, Marianela.
El empleo de bacterias solubilizadoras de fosfato (BSP) para la solubilización de distintas rocas fosfatadas y de otras fuentes de fósforo es una alternativa para incrementar la cantidad de P disponible para las plantas. Se aislaron 147 BSP de la raíz y rizoplano  de diferentes  plantas cultivadas.  El  medio de cultivo  denominado Medio Mínimo para Solubilización de Fosfato Colegio de Postgraduados con MgCl (MMSFCP-Mg) fue el más eficiente,  debido a que en medio sólido se observaron halos de solubilización de hasta 3.8 cm a los  catorce días, y en medio líquido se determinaron hasta   380 mg mL-1  de fosfato soluble a los seis días.   Veintisiete  de las mejores cepas     fueron seleccionadas  con base  en la eficiencia de solubilización de fosfato...
Palavras-chave: Acidos orgánicos; Fosfatasas; Solubilización de fosfato; Inmunodetección; Roca fosfórica; Bacterias solubilizadoras de fosfato; Enfófitas; Organic acids; Phosphatases; Phosphate solubilization; Immunodetection; Rock phosphate; Phosphate solubilizing; Bacteria; Endophyte; Doctorado; Edafología.
Ano: 2010
URL: http://hdl.handle.net/10521/245

Transmission per os du white spot syndrome virus : facteur limitant l'extension de la maladie chez les crustacés marins
Provedor de dados: 5
Autores: Hernandez Herrera, Rosa Idalia.
Penaeid family shrimp constitute the first aquaculture product in the world in terms of commercial value. They are produced in third world countries of the sub-equatorial belt. Among the causes limiting their production, one is the presence of the WSSV (White Spot Syndrome Virus), a pathogenic agent that produces massive mortality. Our aim was to investigate the first stages of the viral infection, in order to be used as target of prophylactic actions. A fish cell line (SSN-1) was used as model to tentatively develop in vitro studies. Only defective particles were produced confirming the high specificity to crustacea of the infection with crustacean virus. Electron microscopy showed structural similarities between the WSSV and B, B2 and Baculo-B viruses of...
Tipo: Text
Palavras-chave: Electron Microscope; Immunodetection; Dot blot; ELISA  sandwich; Cell line SSN1; MrNv; B2 virus; WSSV; Microscopie électronique à transmission; Immunodetection; Dot blot; Sandwich ELISA; Lignée cellulaire SSN1; MrNv; Virus B2; WSSV.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/these-6650.pdf

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA
Provedor de dados: 56
Autores: Lima Barros,A.E.; Almeida,A.M.P.; Carvalho Jr.,L.B.; Azevedo,W.M.
We developed an efficient method to prepare a hybrid inorganic-organic composite based on polyvinyl alcohol (PVA) and polysiloxane using the sol-gel disc technique. Antigen obtained from Yersinia pestis was covalently immobilized onto these discs with glutaraldehyde and used as solid phase in ELISA for antibody detection in serum of rabbits experimentally immunized with plague. Using 1.25 µg antigen per disc, a peroxidase conjugate dilution of 1:4,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. These values are similar to those used for PVA-glutaraldehyde discs, plasticized filter paper discs and the polyaniline-Dacron composite discs. This procedure is comparable to that which utilizes the adsorption of the antigen...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Polysiloxane; Polyvinyl alcohol; Solid phase; Immunodetection; Yersinia pestis; ELISA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400008