Consulta: subjectFacets:"Immunogenicity"
Registros recuperados: 9
Data/hora: 01/06/2024 05:39:26

Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax).
Provedor de dados: 5
Autores: Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text
Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax..
Ano: 2002
URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf

Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) venom obtained by different methods
Provedor de dados: 80
Autores: Ozkan,O.; Kar,S.; Güven,E.; Ergun,G.
Scorpions are venomous arthropods of the class Arachnida and are considered relatives of spiders, ticks and mites. There are approximately 1,500 species of scorpions worldwide, which are characterized by an elongated body and a segmented tail that ends in a venomous stinger. No specific treatment is available for scorpion envenomation, except for the use of antivenom. The current study aimed at comparing protein content and lethality of Androctonus crassicauda venom extracted by two different methods (electric stimulation and maceration of telsons). The LD50 calculated by probit analysis was 1.1mg/kg for venom obtained by electric stimulation and 39.19mg/kg for venom obtained by maceration of telsons. In the electrophoretic analysis, protein bands of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion venoms; Androctonus crassicauda; Gland maceration; Electric stimulation; Immunogenicity; Lethality.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000400013

Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
Provedor de dados: 80
Autores: Miyasato,PA; Ramos,CRR; Abreu,PAE; Dias,WO; Nascimento,C; Ho,PL; Kawano,T.
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni; Cysteine proteinase; Cathepsin L1; Vaccine; Immunogenicity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011

The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells
Provedor de dados: 48
Autores: Zhang,Li; Liu,Dan; Pu,Dan; Wang,Yanwen; Li,Li; He,Yanqi; Li,Yalun; Li,Lei; Li,Weimin.
BACKGROUND: Mesenchymal stem cells (MSCs) are considered the best candidate in stem cells therapy due to their multipotent differentiation ability, low expression of co-stimulatory molecules (CD80, CD86, CD34 and HLA-II) and immunosuppression effects on in vivo immune responses. MSCs were now widely used in clinical trials but received no encourage results. The major problem was the fate of engrafted MSCs in vivo could not be defined. Some studies indicated that MSCs could induce immune response and result in the damage and rejection of MSCs. As toll like receptors (TLRs) are important in inducing of immune responses, in this study we study the role of TLR7 in mediating the immune status of MSCs isolated from umbilical cord. RESULTS: Our results indicated...
Tipo: Journal article
Palavras-chave: Mesenchymal stem cells; Umbilical cord; Toll like receptor 7; Immunogenicity; Pro-inflammatory molecules.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100006

Immunogenicity and safety of Southern Hemisphere inactivated quadrivalent influenza vaccine: a Phase III, open-label study of adults in Brazil
Provedor de dados: 55
Autores: Zerbini,Cristiano A.F.; Ribeiro dos Santos,Rodrigo; Jose Nunes,Maria; Soni,Jyoti; Li,Ping; Jain,Varsha K.; Ofori-Anyinam,Opokua.
Abstract The World Health Organization influenza forecast now includes an influenza B strain from each of the influenza B lineages (B/Yamagata and B/Victoria) for inclusion in seasonal influenza vaccines. Traditional trivalent influenza vaccines include an influenza B strain from one lineage, but because two influenza B lineages frequently co-circulate, the effectiveness of trivalent vaccines may be reduced in seasons of influenza B vaccine-mismatch. Thus, quadrivalent vaccines may potentially reduce the burden of influenza compared with trivalent vaccines.In this Phase III, open-label study, we assessed the immunogenicity and safety of Southern Hemisphere inactivated quadrivalent influenza vaccine (Fluarix™ Tetra) in Brazilian adults (NCT02369341). The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quadrivalent; Influenza; Vaccine; Immunogenicity; Safety; Brazil; Adults.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100063

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in elderly residents of a long-term care facility
Provedor de dados: 55
Autores: Valenzuela B.,M. Teresa; Altuzarra H.,Rogelio; Trucco A.,Olivia; Villegas R.,Rodrigo; Inostroza S.,Jaime; Granata S.,Paulo; Fleiderman V.,José; Maggi C.,Leonardo.
S. pneumoniae is a significant cause of community-acquired pneumonia in the elderly, and accounts for the majority of the pneumonia deaths among the elderly. We conducted this randomized double-blind study to evaluate the immune response to a 23-valent pneumococcal polysaccharide vaccine and the persistence of antibodies two years after the vaccination in an elderly population in Santiago, Chile. A total of 118 elderly nursing home residents received either the pneumococcal or a tetanus control vaccine. Serum samples were taken at enrolment, at two months, and at two years post-vaccination. Pre-vaccination anti-pneumococcal antibody geometric mean concentrations (GMC) were similar in both study groups, with increased levels of antibodies found only against...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Elderly; Immunogenicity; Antibodies.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300005

Attenuation and immunogenicity of recombinant yellow fever 17D-dengue type 2 virus for rhesus monkeys
Provedor de dados: 56
Autores: Galler,R.; Marchevsky,R.S.; Caride,E.; Almeida,L.F.C.; Yamamura,A.M.Y.; Jabor,A.V.; Motta,M.C.A.; Bonaldo,M.C.; Coutinho,E.S.F.; Freire,M.S.
A chimeric yellow fever (YF)-dengue serotype 2 (dengue 2) virus was constructed by replacing the premembrane and envelope genes of the YF 17D virus with those from dengue 2 virus strains of Southeast Asian genotype. The virus grew to high titers in Vero cells and, after passage 2, was used for immunogenicity and attenuation studies in rhesus monkeys. Subcutaneous immunization of naive rhesus monkeys with the 17D-D2 chimeric virus induced a neutralizing antibody response associated with the protection of 6 of 7 monkeys against viremia by wild-type dengue 2 virus. Neutralizing antibody titers to dengue 2 were significantly lower in YF-immune animals than in YF-naive monkeys and protection against challenge with wild-type dengue 2 virus was observed in only 2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: YF 17D virus recombinants; Dengue vaccine; Immunogenicity; Attenuation; Rhesus monkeys.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200012

Prediction of exposed domains of envelope glycoprotein in Indian HIV-1 isolates and experimental confirmation of their immunogenicity in humans
Provedor de dados: 56
Autores: Mohabatkar,H.; Kar,S.K.
We describe the impact of subtype differences on the seroreactivity of linear antigenic epitopes in envelope glycoprotein of HIV-1 isolates from different geographical locations. By computer analysis, we predicted potential antigenic sites of envelope glycoprotein (gp120 and gp4l) of this virus. For this purpose, after fetching sequences of proteins of interest from data banks, values of hydrophilicity, flexibility, accessibility, inverted hydrophobicity, and secondary structure were considered. We identified several potential antigenic epitopes in a B subtype strain of envelope glycoprotein of HIV-1 (IIIB). Solid- phase peptide synthesis methods of Merrifield and Fmoc chemistry were used for synthesizing peptides. These synthetic peptides corresponded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human immunodeficiency virus; Glycoprotein; Epitope; Immunogenicity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500008

Structural, functional and immunological studies on a polymeric bacterial protein
Provedor de dados: 56
Autores: Baldi,P.C.; Velikovsky,C.A.; Braden,B.C.; Giambartolomei,G.H.; Fossati,C.A.; Goldbaum,F.A.
The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazine-synthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella lumazine synthase; X-ray structure; Immunogenicity; Brucellosis detection; Bacillus subtilis lumazine synthase.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700003