Consulta: subjectFacets:"In silico"
Registros recuperados: 10
Data/hora: 09/06/2024 15:42:07

Two LTR retrotransposon elements within the abscisic acid gene cluster in Botrytis cinerea B05.10, but not in SAS56
Provedor de dados: 69
Autores: Zhao,Ming; Zhou,Jin yan; Tan,You jiu; Song,Wei wei; Li,Zhi dong; Tan,Hong.
The plant hormone abscisic acid has huge economic potential and can be applied in agriculture and forestry for it is considered to be involved in plant resistance to stresses such as cold, heat, salinity, drought, pathogens and wounding. Now overproducing strains of Botrytis cinerea are used for biotechnological production of abscisic acid. An LTR retrotransposon, Boty-aba, and a solo LTR were identified by in silico genomic sequence analysis, and both were detected within the abscisic acid gene cluster in B. cinerea B05.10, but not in B. cinerea SAS56. Boty-aba contains a pair of LTRs and two internal genes. The LTRs and the first gene have features characteristic of Ty3/gypsy LTR retrotransposons. The second gene is a novel gene, named brtn, which...
Tipo: Journal article
Palavras-chave: Botrytis cinerea; Genomic sequence analysis; In silico; LTR retrotransposon.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100007

In silico identification of coffee genome expressed sequences potentially associated with resistance to diseases
Provedor de dados: 74
Autores: Alvarenga,Samuel Mazzinghy; Caixeta,Eveline Teixeira; Hufnagel,Bárbara; Thiebaut,Flávia; Maciel-Zambolim,Eunize; Zambolimand,Laércio; Sakiyama,Ney Sussumu.
Sequences potentially associated with coffee resistance to diseases were identified by in silico analyses using the database of the Brazilian Coffee Genome Project (BCGP). Keywords corresponding to plant resistance mechanisms to pathogens identified in the literature were used as baits for data mining. Expressed sequence tags (ESTs) related to each of these keywords were identified with tools available in the BCGP bioinformatics platform. A total of 11,300 ESTs were mined. These ESTs were clustered and formed 979 EST-contigs with similarities to chitinases, kinases, cytochrome P450 and nucleotide binding site-leucine rich repeat (NBS-LRR) proteins, as well as with proteins related to disease resistance, pathogenesis, hypersensitivity response (HR) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Data mining; ESTs; Genomics; In silico; Bioinformatics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400031

Citrus plastid-related gene profiling based on expressed sequence tag analyses
Provedor de dados: 74
Autores: Calsa Jr.,Tercilio; Figueira,Antonio.
Plastid-related sequences, derived from putative nuclear or plastome genes, were searched in a large collection of expressed sequence tags (ESTs) and genomic sequences from the Citrus Biotechnology initiative in Brazil. The identified putative Citrus chloroplast gene sequences were compared to those from Arabidopsis, Eucalyptus and Pinus. Differential expression profiling for plastid-directed nuclear-encoded proteins and photosynthesis-related gene expression variation between Citrus sinensis and Citrus reticulata, when inoculated or not with Xylella fastidiosa, were also analyzed. Presumed Citrus plastome regions were more similar to Eucalyptus. Some putative genes appeared to be preferentially expressed in vegetative tissues (leaves and bark) or in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus variegated chlorosis; In silico; Sweet orange; Tangerine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500013

Análise in silico da expressão diferencial de genes em Arachis magna sob estresse hídrico.
Provedor de dados: 14
Autores: BRASILEIRO, A. C. M.; SANTOS, C. M. R.; MORGANTE, C. V.; MARTINS, A. da C. Q.; DA SILVA, F. R.; ARAÚJO, A. C. G. de; BERTIOLI, D. J.; LEAL-BERTIOLI, S. C. de M.; GUIMARÃES, P. M.
bitstream/CENARGEN-2009-09/31580/3/bp229_1208.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Arachis magna; Melhoramento genético; In silico; Estresse hídrico.; Análise.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/191229

Análise in silico da expressão gênica diferencial de Arachis stenosperma inoculado com Meloidogyne arenaria.
Provedor de dados: 14
Autores: GUIMARÃES, P. M.; PROITE, K.; LAEL-BERTIOLI, S. C.; BERTIOLI, D.
bitstream/CENARGEN/26629/1/bp085.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Arachis stenosperma; Expressão gênica diferencial; In silico; Ground nut; Disease; Differential genic expression; Analysis.; Amendoim; Análise; Doença; Meloidoginose; Meloidogyne Arenaria.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187039

In silico analysis of binding interaction of conantokins with NMDA receptors for potential therapeutic use in Alzheimer’s disease
Provedor de dados: 80
Autores: Batool,Maleeha Waqar and Sidra.
Abstract  Background  The N-methyl-D-aspartate (NMDA) receptors are glutamate receptors that play vital roles in central nervous system development and are involved in synaptic plasticity, which is an essential process for learning and memory. The subunit N-methyl D-aspartate receptor subtype 2B (NR2B) is the chief excitatory neurotransmitter receptor in the mammalian brain. Disturbances in the neurotransmission mediated by the NMDA receptor are caused by its overexposure to glutamate neurotransmitter and can be treated by its binding to an antagonist. Among several antagonists, conantokins from cone snails are reported to bind to NMDA receptors.  Methods  This study was designed to analyze the binding mode of conantokins with NMDA receptors in both humans...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: N-methyl-D-aspartate; Glutamate; Synaptic plasticity; NR2B; Neurotransmitter; Antagonists; Conantokins; Docking; In silico; Alzheimer’s dieases.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100317

Optimización de la amplificación por PCR del gen de la endoglucanasa de Ralstonia solanacearum
Provedor de dados: 100
Autores: Naranjo,Eber; Otero,Adriana; Martínez-Zubiaur,Yamila.
El esquema de clasificación actual del agente etiológico de la marchitez bacteriana (Ralstonia solanacearum) se basa en el análisis de la secuencia del gen de la endoglucanasa de esta bacteria. El objetivo de este estudio fue optimizar la amplificación por PCR de este gen. Las condiciones de PCR fueron predeterminadas in silico con el empleo del programa OLIGO versión 7.53. Se estimó el efecto de la concentración celular en la eficiencia de la amplificación con diluciones seriadas de suspensiones bacterianas de R. solanacearum. Se evaluó un protocolo de PCR de dos pasos con el empleo del kit comercial GoTaq® DNA Polimerasa para los ensayos in vitro. La concentración celular fue un factor crítico para la amplificación con un valor óptimo de 10(8) UFC.ml-1....
Tipo: Journal article
Palavras-chave: Marchitez bacteriana; In silico; In vitro.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522013000300008

Molecular characteristics, recombinant expression and activity detection of OsGSTL1 from rice
Provedor de dados: 52
Autores: Hu,Tingzhang; Yang,Yongwei; Tan,Lili; Yang,Junnian; Wu,Yingmei.
The mRNA of OsGSTL1 was detected in the roots and leaves of rice plants at seedling and tillering stages, and their roots, leaves and panicles at the heading stage. The full-length open reading frame of OsGSTL1 cDNA was 732 bp and encoded a putative polypeptide of 243 amino acids with a calculated molecular mass of 27.30 kDa and a theoretical pI of 5.50. The protein sequences of OsGSTL1 exhibited typical feature of the lambda class GST, which contained the conserved domain "GST_C_Lambda" in C-terminal alpha helical domain and a highly conserved Cys42 in active center. In silico predictions showed that the OsGSTL1 protein was strongly hydrophilic. The phylogenetic analysis revealed OsGSTL1 belonged to monocots subgroup and was closer to IN2-1 of Z. may. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione S-transferase; GST activity; In silico; Oryza sativa L.; Recombinant expression; Semi-quantitative RT-PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100011

Drug discovery and computational strategies in the multitarget drugs era
Provedor de dados: 60
Autores: Viana,Jéssika de Oliveira; Félix,Mayara Barbalho; Maia,Mayara dos Santos; Serafim,Vanessa de Lima; Scotti,Luciana; Scotti,Marcus Tullius.
The pharmaceutical industry is increasingly joining chemoinformatics in the search for the development of new drugs to be used in the treatment of diseases. These computational studies have the advantage of being less expensive and optimize the study time, and thus the interest in this area is increasing. Among the techniques used is the development of multitarget directed ligands (MTDLs), which has become an ascending technique, mainly due to the improvement in the quality of treatment involving several drugs. Multitarget therapy is more effective than traditional drug therapy that emphasizes maximum selectivity for a single target. In this review a multitarget drug survey was carried out as a promising strategy in several important diseases: neglected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drug design; Multitarget compounds; In silico; Diseases.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700409

ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple Glomerella leaf spot
Provedor de dados: 65
Autores: Giaretta,Diorvania Ribeiro; Bogo,Amauri; Coelho,Cileide Maria Medeiros; Guidolin,Altamir Frederico; Dantas,Adriana Cibele de Mesquita; Gomes,Eliane Aparecida.
Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pineapple guava; Citrus; Molecular phylogeny; In silico; Phylogenetic tree.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400010