Consulta: subjectFacets:"In vitro fertilization"
Registros recuperados: 11
Data/hora: 09/06/2024 18:02:26

Germinação "in vitro" de mogno.
Provedor de dados: 14
Autores: LOPES, S. da C.; LAMEIRA, O. A.; FORTES, G. R. L.; NOGUEIRA, R. C.; LEÃO, N. V. M.
bitstream/item/42026/1/BolPesquisa30.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Germinação in vitro; Pará; Brasil; Emergence.; Cultura de Tecido; Emergência; Mogno; Swietenia Macrophylla.; Substrato de Cultura; Amazonia; Culture media; In vitro fertilization; Tissue culture.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382708

Sperm handling in aquatic animals for artificial reproduction
Provedor de dados: 5
Autores: Beirão, José; Boulais, Myrina; Gallego, Victor; O’brien, Justine K.; Peixoto, Silvio; Robeck, Todd R.; Cabrita, Elsa.
Artificial reproduction involves collection and handling of gametes in a way that secures their quality and maximizes the fertilization outcome. In addition to initial sperm quality, numerous steps can affect the final result of fertilization, from the sperm collection process until gamete mixing (or co-incubation) when spermatozoon enters or fuses with the oocyte. In this review, we summarize the whole process of sperm handling, from collection until fertilization for fish, penaeid shrimp, bivalve mollusks and marine mammals. To obtain sperm from captive animals, techniques vary widely across taxa, and include stripping by abdominal massage or testis surgical removal in fish, spermatophore collection in penaeid shrimps, gonadal scarification or...
Tipo: Text
Palavras-chave: Sperm extraction; Spermatozoa:oocyte ratio; In vitro fertilization; In vitro reproduction; Assisted reproduction.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00492/60411/63847.pdf

Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
Provedor de dados: 96
Autores: Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano.
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003

Simulation and economic analysis of bovine sex selection
Provedor de dados: 96
Autores: Beltrame,Renato Travassos; Quirino,Celia Raquel; Barioni,Luís Gustavo; Lima,Vera Fernanda Martins Hossepian de.
A simulation model implemented in the programming software Delphi XE® was applied to evaluate sex selection in bovine. The hypothesis under investigation was that a dynamic model with stochastic and deterministic elements could detect the sexed semen technique to minimize pregnancy cost and to determine the adequate number of recipients required for in vivo (ET) and in vitro embryo production (IVP) in the proposed scenarios. Sex selection was compared through semen sexed using flow cytometry (C1) and density gradient centrifugation techniques (C2) in ET and IVP. Sensibility analyses were used to identify the adequate number of recipients for each scenario. This number was reinserted into the model to determine the biological and financial values that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; In vitro fertilization; Modeling; Stochastic simulation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000500003

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos
Provedor de dados: 63
Autores: Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003

ผลของการเสริม Ethidium bromide หรือ CuSO4 ในปฏิกิริยา Loop-mediated isothermal amplification (LAMP) ต่อการเพิ่มความแม่นยำในการคัดเพศตัวอ่อนโคเนื้อ
Provedor de dados: 106
Autores: Saruttiwong Boonkong; Chainarong Navanukraw; Tossapol Moonmanee; Jiratti Thammasiri; Vilaivan Khanthusaeng; Aree Kraisoon.
The study was conducted to determine effect of EB or CS supplements in LAMP reaction on greater accuracy of sexing cattle embryos. Bovine ovaries were collected from the slaughterhouse, oocytes were aspirated from follicles then were evaluated morphology. Oocytes were cultured in TCM-199 at 38.5 ºC 24 h. For in vitro fertilization (IVF), oocytes were co-incubated with spermatozoa at 38.5 ºC 24 h. Fertilization rate was evaluated and then the embryos were in vitro cultured until morula stage. Then, embryo samples were frozen and stored at -196 ºC. Frozen embryos (n=35) were randomly extracted for total cellular DNA and amplified by PCR method. DNA samples were stored at -20 ºC. Sexing was determined by modification of LAMP method with either 1mM EB or 1M CS...
Tipo: PhysicalObject
Palavras-chave: Cattle; Bovine; Oocyte; LAMP reaction; Loop-mediated isothermal amplificatio; Ethidium bromide; EB; CuSO4; CS; DNA; Sexing embryo; In vitro fertilization; IVF; โคเนื้อ; โอโอไซต์; ลักษณะทางสัณฐานวิทยา; การคัดเพศตัวอ่อน; เทคนิคการคัดเพศ; ดีเอ็นเอ; ความแม่นยำ.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5813

Effect of insulin-like growth factor-1 during in vitro oocyte maturation and in vitro culture of bovine embryos
Provedor de dados: 45
Autores: Quetglas,M.D.; Coelho,L.A.; Garcia,J.M.; Oliveira Filho,E.B.; Esper,C.R.
The effects of insulin-like growth factor-I (IGF-I) on in vitro maturation (IVM) (experiment I) and on in vitro embryo development (experiment II) of bovine oocytes fertilized in vitro, were evaluated in terms of cleavage (CR), blastocyst (BR) and hatching (HR) rates. For IVM, immature cumulus-oocyte complexes were cultured in TCM-199 medium supplemented with Hepes, sodium bicarbonate, sodium pyruvate, additives, fetal calf serum (B-199 medium) and gonadotropins (14 U/ml PMSG and 7 U/ml hCG). For embryo development, the oocytes/zygotes were cultured in B-199 medium with bovine oviduct epithelial cells in suspension under silicon oil. Treatments for in vitro culture conditions for both experiments were: 1- B-199 + 200 ng/ml IGF-I; 2- B-199 + 100 ng/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Cleavage rate; IGF-I; In vitro maturation; In vitro fertilization.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000200014

Oocyte aspiration and in vitro embryo production in Jersey cows with selenium-supplemented diet
Provedor de dados: 45
Autores: Moraes,G.V.; Azevedo,J.R.; Carneiro,T.C.; Cavalieri,F.L.B.; Mataveli,M.; Rossi,R.M.; Santos,T.C.
The effects of selenium (Se) in Jersey cows' diet on the aspiration of oocytes and production of embryos in vitro were studied. Groups with five Jersey cows received 3.2mg or 9.6mg Se daily, provided in the feed concentrate. Six follicular aspirations were carried out every 15 days, using only the last 5. The oocytes were classified, and standard procedures were carried out for maturation, fertilization and cultivation. The total number of oocytes (35.11±2.65 vs 23.10±2.16) and degree 1 oocytes (11.61±2.65 vs 4.75±0.97) were higher in the group that received 9.6mg Se and the quantity of naked oocytes (3.23±0.87 vs 6.22±1.18) was lower in this group. The aspirated oocytes from the cows treated with 9.6mg Se/day resulted in higher (P&lt;0.05) embryo...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle dairy; Antioxidant; Glutathione peroxidase; In vitro fertilization; Oocyte quality.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000400001

Aspects of energetic substrate metabolism of in vitro and in vivo bovine embryos
Provedor de dados: 56
Autores: de Souza,D.K.; Salles,L.P.; Rosa e Silva,A.A.M.
Although the metabolism of early bovine embryos has not been fully elucidated, several publications have addressed this important issue to improve culture conditions for cattle reproductive biotechnologies, with the ultimate goal of producing in vitro embryos similar in quality to those developing in vivo. Here, we review general aspects of bovine embryo metabolism in vitro and in vivo, and discuss the use of metabolic analysis of embryos produced in vitro to assess viability and predict a viable pregnancy after transference to the female tract.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine pregnancy rates; Embryo metabolism; Metabolism profile; In vitro fertilization; Embryo culture conditions; In vivo embryo.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300191

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes
Provedor de dados: 56
Autores: Costa,L.O.B.; Mendes,M.C.; Ferriani,R.A.; Moura,M.D.; Reis,R.M.; Silva de Sá,M.F.
The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Steroid in follicular fluid; Maturity of oocytes; In vitro fertilization.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100021

Acceptable microorganisms concentration in a semen sample for in vitro embryo production
Provedor de dados: 58
Autores: D'Angelo,Magali; Pavão,Danielle L.; Melo,Gisele Martins; Rojas,Natali; Souza,Rosimeire Joaquim; Athayde,Christiani; Galuppo,Andrea Giannotti; Pinheiro,Eliana Scarcelli.
The aim of this study was to report that the acceptable concentration of microorganisms in a semen sample for insemination may not be safe for an in vitro fertilization procedure. It seems that the semen sample should be completely germ-free, because of the excellent microorganism proliferation condition promoted by the in vitro environment.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Semen; Microorganisms; In vitro fertilization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400031