Consulta: subjectFacets:"In vivo"
Registros recuperados: 18
Data/hora: 06/06/2024 02:59:07

Identification of differentially expressed genes of Xanthomonas axonopodis pv. citri by representational difference analysis of cDNA
Provedor de dados: 74
Autores: Mehta,Angela; Rosato,Yoko B.
Xanthomonas axonopodis pv. citri is a phytopathogenic bacterium responsible for citrus canker, a serious disease which causes severe losses in citriculture around the world. In this study we report the differential expression of X. axonopodis pv. citri in response to specific treatments by using Representational Difference Analysis of cDNA (cDNA RDA). cDNAs from X. axonopodis pv. citri cultured in the presence of leaf extract of the host plant (Citrus sinensis), in vivo, as well as in the complex medium were hybridized against cDNA of the bacterium grown in the minimal medium. Sequencing of the difference products obtained after the second and third hybridizations revealed a total of 37 distinct genes identified by homology searches in the genome of X....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Citrus canker; Differential expression; Leaf extract; In vivo.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100024

Seasonal changes in carbohydrate metabolism and its relationship with summer mortality of Pacific oyster Crassostrea gigas (Thunberg) in Marennes-Ole'ron bay (France)
Provedor de dados: 5
Autores: Soletchnik, Patrick; Faury, Nicole; Goulletquer, Philippe.
This paper investigates the biological responses of Crassostrea gigas under traditional culture conditions on a mudflat in Marennes-Oleron bay. Summer mortality has been regularly observed in recent years in oysters reared using "on bottom" culture conditions. The present study attempts to provide a better understanding of the mortality phenomenon through biological parameters. Classical 'field parameters' such as mortality and growth rates, and quality index (dry meat weight/ dry shell weight x 1000) were monitored. Additional parameters, as biochemical composition of oyster meat and glucose incorporation capacity, were included as potential new bioindicators. The work highlighted a critical timing (May-June) preceding the summer mortality and...
Tipo: Text
Palavras-chave: Marennes Oleron bay; Glycogenolysis; In vivo; Glycogen incorporation; Maturation; Summer mortality; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1116.pdf

In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families.
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf

Decreased mitochondrial metabolic requirements in fasting animals carry an oxidative cost
Provedor de dados: 5
Autores: Salin, Karine; Villasevil, Eugenia M.; Anderson, Graeme J.; Auer, Sonya K.; Selman, Colin; Hartley, Richard C.; Mullen, William; Chinopoulos, Christos; Metcalfe, Neil B.
Many animals experience periods of food shortage in their natural environment. It has been hypothesised that the metabolic responses of animals to naturally‐occurring periods of food deprivation may have long‐term negative impacts on their subsequent life‐history. In particular, reductions in energy requirements in response to fasting may help preserve limited resources but potentially come at a cost of increased oxidative stress. However, little is known about this trade‐off since studies of energy metabolism are generally conducted separately from those of oxidative stress. Using a novel approach that combines measurements of mitochondrial function with in vivo levels of hydrogen peroxide (H2O2) in brown trout (Salmo trutta), we show here that fasting...
Tipo: Text
Palavras-chave: High-resolution respirometry; In vivo; Liver atrophy; MitoB probe; Mitochondrial respiratory state.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00449/56022/57540.pdf

Activity of the antiarrhythmic drug amiodarone against Leishmania (L.) infantum: an in vitro and in vivo approach
Provedor de dados: 80
Autores: Pinto,Erika G.; Tempone,Andre G.
Abstract  Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market.  Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity.  Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320

Evaluation of inhibitory effects of dipyridamole against bovine and equine piroplasmosis
Provedor de dados: 19
Autores: Tuvshintulga, B.; Sivakumar, T.; Yokoyama, N.; Igarashi, I.
Dipyridamole, an antiplatelet drug used for the secondary prevention and treatment of stroke, also has antiplasmodial activity and enhances the activity of chloroquine. We evaluated the inhibitory effects of dipyridamole on the growths of Babesia and Theileria parasites. The growth of B. bovis and T. equi was significantly inhibited at a 25 μM concentration of dipyridamole, while B. bigemina and B. caballi were significantly inhibited at 50 μM and 1 μM concentrations of dipyridamole, respectively, on day 3 of cultivation. The half maximal inhibitory concentration (IC50) of dipyridamole was calculated at 39.5±4.5, 26.3±10.9, 13.2±3.6, and 23.5±0.5 μM on the growth of B. bovis, B. bigemina, B. caballi, and T. equi, respectively. In a viability assay, B....
Palavras-chave: Dipyridamole; Babesia; Theileria; In vitro; In vivo.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4497

Evaluation of inhibitory effect of methylene blue against Babesia and Theileria parasites
Provedor de dados: 19
Autores: Tuvshintulga, B.; Sivakumar, T.; Salama, A.A.; Yokoyama, N.; Igarashi, I; 五十嵐, 郁男; 横山, 直明.
Methylene blue, the first fully synthetic drug, has been widely used in medical treatments. A few decades ago, this drug was used as an antimalarial agent. In this study, the in vitro inhibitory effect of methylene blue on Babesia bovis, B. bigemina, B. caballi, and Theileria equi (B. equi) and the in vivo inhibitory effect on B. microti were evaluated. Methylene blue significantly inhibited the growth of B. bovis, B. caballi, and T. equi at a 0.1 μM concentration, while B. bigemina was significantly inhibited at 0.01 μM, on day 3 of cultivation. The half maximal inhibitory concentrations (IC50) of methylene blue against B. bovis, B. bigemina, B. caballi, and T. equi were calculated as 0.83±0.02, 0.68±0.09, 0.54±0.14, and 0.49±0.06 μM, respectively. The...
Palavras-chave: Methylene blue; Babesia; Theileria; In vitro; In vivo.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4242

Intraluteal Release of Prostaglandin F2α and E2 During Corpora Lutea Development in the Cow
Provedor de dados: 19
Autores: KOBAYASHI, Shu-ichi; ACOSTA, Tomas J.; HAYASHI, Kanako; BERISHA, Bajram; OZAWA, Takahiro; OHTANI, Masayuki; SCHAMS, Dieter; MIYAMOTO, Akio; 宮本, 明夫.
It is well known that prostaglandin (PG) F2α and PGE2 are actively produced by bovine early corpus luteum (CL) at higher levels than in the CL of later stages. However, there is no in vivo information about the local secretion of PGs within bovine developing CL so far. Thus, the objective of the present study was to determine in detail the real-time changes in PGF2α and PGE2 secretion within developing CL. A microdialysis system (MDS) was surgically implanted into the newly formed CL of 6 cows on Day 3 after GnRH administration (about 1.5 days after ovulation) to induce superovulation following FSH treatment. Both PGF2α and PGE2 release were high during the first 24 h within developing CL but decreased thereafter. These profiles were well reflected in the...
Palavras-chave: PGF2α; PGE2; Developing CL; Cow; In vivo.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2974

Evaluación in vivo de la actividad antimalárica de 25 plantas provenientes de una Reserva de Conservación Biológica de Costa Rica
Provedor de dados: 97
Autores: CHINCHILLA-CARMONA,MISAEL; VALERIO-CAMPOS,IDALIA; SÁNCHEZ-PORRAS,RONALD; MORA-CHAVES,VÍCTOR; BAGNARELLO-MADRIGAL,VANESSA; MARTÍNEZ-ESQUIVEL,LAURA; GONZÁLEZ-PANIAGUA,ANTONIETA; VANEGAS,JUAN CARLOS.
Se realizó una evaluación in vivo de la actividad antimalárica de las hojas, flores, frutos, corteza y raíz de 25 plantas de la Reserva Biológica Alberto Manuel Brenes (REBAMB), situada en San Ramón, Alajuela, Costa Rica. Las plantas estudiadas fueron Aphelandra aurantiaca (Scheidw.) Lindl., Aphelandra tridentata Hemsl. (Acanthaceae), Xanthosoma undipes (K. Koch &amp;amp; C.D. Bouché) K. Koch. (Araceae), Iriartea deltoidea Ruiz &amp;amp; Pav. (Arecaceae), Neurolaena lobata (L.) Cass. (Asteraceae), Lonchocarpus pentaphyllus (Poir.) Kunth ex DC., Pterocarpus hayesii Hemsl., Senna papillosa (Britton &amp;amp; Rose) H.S. Irwin &amp;amp; Barneby., Cinnamomum chavarrianum (Hammel) Kosterm. (Fabaceae), Nectandra membranacea (Sw.) Griseb., Persea povedae W.C....
Tipo: Journal article
Palavras-chave: Antimaláricos; Costa Rica; In vivo; Plantas; Plasmodium berghei.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2011000100009

Response of seeds and pollen of Onobrychis viciifolia and Onobrychis oxyodonta var. armena to NaCl stress
Provedor de dados: 63
Autores: Beyaz,Ramazan; Kaya,Gamze; Cocu,Sati; Sancak,Cengiz.
Sainfoin (Onobrychis viciifolia Scop.) is an important forage legume crop with 52 species adapted to dry and poor soils in Turkey, but little is known about the effects of salinity on germination and seedling growth in arid and semiarid regions suffering from salinity problem. The seeds and pollen of two species of sainfoin O. viciifolia and O. oxyodonta var. armena (Syn: O. armena) were exposed to 0, 5, 10, 20 and 30 dS m-1 of NaCl under in vivo and in vitro conditions and evaluated for germination under salt stress by comparing germination percentage, mean germination time, root and shoot length, fresh and dry seedling weight and dry matter. Increased salinity levels generally resulted in decrease in all traits except time to germination, dry seedling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vivo; In vitro; Sainfoin; Salinity; Germination.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400013

Multiplicación clonal in vivo e in vitro de la especie forestal nativa Aniba perutilis Hemsl.
Provedor de dados: 109
Autores: Delgado García,Lina Marcela; Hoyos Sánchez,Rodrigo Alberto.
Se desarrolló una metodología para el establecimiento in vitro e in vivo de Aniba perutilis (comino crespo), árbol colombiano en peligro crítico de extinción. En la primera etapa, se sometieron semillas de este árbol a la acción de los desinfectantes NaOCl, HgCl2 y AgNO3 para su germinación aséptica. Para inducir la formación de brotes se empleó el medio WPM y se evaluó la acción de las fitohormonas BAP, KIN, AIA, ANA y AG3 en diferentes concentraciones. Para la etapa de enraizamiento se emplearon ANA, AIB, y los medios de cultivo MS y WPM, ambos con sales reducidas a la mitad y combinados con diferentes concentraciones de AIA y ANA. En la etapa de multiplicación in vivo se emplearon plántulas establecidas en vivero para evaluar tres tamaños de explantes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comino crespo; Establecimiento in vitro; In vivo; Mini-esquejes; Recalcitrancia.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122016000200013

Ultrastructural features of Mimulus aurantiacus (Scrophulariaceae) pollen tubes in vivo
Provedor de dados: 42
Autores: Ekici,Nuran; Dane,Feruzan; Olgun,Göksel.
The aim of this study is to give information on ultrastructure of in vivo pollen tubes of Mimulus aurantiacus which were collected from the Botanical Garden of the University of California at Berkeley. Materials were prepared according to electron microscopy methods and examined under Zeiss electron microscope. Four zones were examined in the pollen tubes of Mimulus aurantiacus. APICAL ZONE: Mitochondria, smooth endoplasmic reticulum, rough endoplasmic reticulum, dictyosomes and secretory vesicles were observed. SUBAPICAL ZONE: This area contained abundant rough endoplasmic reticulum and occasionally some smooth endoplasmic reticulum. The polysomes, mitochondria, proplastids that contain starch, small vacuoles and a few lipid bodies were detected. NUCLEAR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vivo; Mimulus; Pollen tube; Ultrastructure.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000100005

Avaliação de métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para ruminantes
Provedor de dados: 45
Autores: Silveira,M.F.; Kozloski,G.V.; Mesquita,F.R.; Farenzena,R.; Senger,C.C.D.; Brondani,I.L.
Foi avaliada a acurácia dos métodos laboratoriais para estimar a digestibilidade e o valor energético de dietas para bovinos de corte. As dietas experimentais foram isonitrogenadas e compostas por silagem de milho e 25, 40, 55 ou 70% de concentrado. Os valores de digestibilidade medidos in vivo foram comparados com os obtidos nos ensaios in situ, in vitro e in vitro/gases, e com valores estimados a partir de equações matemáticas baseadas na composição química das dietas. O ensaio in vivo foi realizado com quatro bovinos em delineamento em quadrado latino 4×4. Quatro animais foram usados para o ensaio in situ e quatro corridas foram realizadas para os ensaios in vitro. Não houve interação significativa de dietas versus métodos. As taxas de degradação...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ruminante; Digestibilidade; Equações; In situ; In vitro; In vivo.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000200021

Expression and Localization of Olfactory Receptor AcerOr1 from Chinese Honey Bee, Apis cerana cerana (Hymenoptera, Apidae)
Provedor de dados: 52
Autores: Guo,Lina.
ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apis cerana cerana; Olfactory receptor; AcerOr1; Expression; Localization; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440

Antitumor activity of C-phycocyanin from Arthronema africanum (Cyanophyceae)
Provedor de dados: 52
Autores: Gardeva,Elena; Toshkova,Reneta; Yossifova,Liliya; Minkova,Kaledona; Ivanova,Natalia; Gigova,Liliana.
Pure C-phycocyanin (C-PC) was isolated from Arthronema africanumto evaluate its potential antitumor effects in vivo and in vitro. Experimental myeloid Graffi tumor in hamsters was used as a model. The cell proliferation assay showed that C-PC treatment, at concentration of 100 µg mL-1 for 24 h, significantly inhibited the growth of Graffi tumor cells (51.4% viability). Agarose gel electrophoresis of the genomic DNA of treated cells displayed time-and concentration-dependent fragmentation pattern, typical for apoptosis. Apoptotic process was related to the increase in cellular manganese and copper/zinc superoxide dismutases and glutathione reductase activities, coupled with a low catalase activity. In vivo C-PC administration (5.0 mg kg-1 body weight)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antitumor activity; Arthronema africanum; In vivo; In vitro; C-pycocyanin; Myeloid Graffi tumor.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500675

Synthesis and anti-myocarditis activity in a multifunctional lanthanide microporous metal-organic framework with 1D helical chain building units
Provedor de dados: 56
Autores: Hong,Chenglv; Zhou,Xinlang; Huang,Weijian; Shan,Peiren; Dong,Fengquan.
A new microporous lanthanide metal-organic framework, {[Yb(BTB)(H2O) (DEF)2}n (1, DEF=N,N-Diethylformamide), with 1D nano-sized channels has been constructed by bridging helical chain secondary building units with 1,3,5-benzenetrisbenzoic acid (H3BTB) ligand. Structural characterization suggests that this complex crystallizes in the hexagonal space group P6122 and possesses 1D triangular channels with coordinated water molecules pointing to the channel center. In addition, anti-myocarditis properties of compound 1 were evaluated in vivo. The results showed that compound 1 can improve hemodynamic parameters of, and it may be a good therapeutic option for heart failure in the future.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metal-organic framework; Anti-myocarditis; In vivo.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300604

Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study
Provedor de dados: 56
Autores: Pan,Hong; Leung,Kevin K.C.; Ng,Enders Kwok Wai.
The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: “LigaSure-L-1” and “LigaSure-L-2”, with low energy output level with 1 or 2 device-activated tissue sealing times, and “LigaSure-M” and “LigaSure-H”, with medium or high energy output level. As controls, automatically stapled (GIA group) and hand-sewn (suture group) anastomoses were utilized in 3 and 2 animals, respectively. There was no statistical difference in the overall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bowel anastomosis; Tissue fusion technology; Porcine; In vivo; Suture; Staple.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500605

In vivo inhibitory effect of suberoylanilide hydroxamic acid combined with sorafenib on human hepatocellular carcinoma cells
Provedor de dados: 60
Autores: Hao,Dalin; Deng,Fang; Shi,Hong; Wang,Hongsheng; Xiao,Fubin; Sun,Chengxue; Xu,Yansong; Li,Peng.
The present study aimed to investigate the in vivo inhibitory effect of histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) combined with sorafenib on human hepatocellular carcinoma HCCLM3 cells. The nude mice transplanted with HCCLM3 cells were randomly divided into control, SAHA, sorafenib and SAHA+sorafenib groups. The nude mice in the later 3 groups were intragastrically administrated with SAHA (10 mg·kg-1·day-1), sorafenib (10 mg·kg-1·day-1) and SAHA (10 mg·kg-1·day-1) combined with sorafenib (10 mg·kg-1·day-1), respectively, for successive 20 days. Finally, the inhibition rate of tumor was measured. The expressions of MEK1/2, p-ERK1/2, Cyclin D1, Bcl-2, Bax, p53, MMP-2, MMP-9 and uPA in tumor tissues were determined. Results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Suberoylanilide hydroxamic acid; HCCLM3; Inhibitory; In vivo.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100528