Consulta: subjectFacets:"Inclusion body"
Registros recuperados: 2
Data/hora: 01/06/2024 11:53:16

Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
Provedor de dados: 69
Autores: Kim,Jong-Wan; Yoe,Jeehyun; Lee,Gil Ho; Yoe,Sung Moon.
The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95% purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for...
Tipo: Journal article
Palavras-chave: Antibacterial activity; Inclusion body; Lysozyme; On-column refolding; Recombinant expression; Spodoptera litura.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300006

Pathology of naturally occurring porcine adenovirus type 4 infection in Japan
Provedor de dados: 19
Autores: Agungpriyono, Dewi Ratih; Nakagawa, Michio; Morozumi, Tetsuo; Yoshihara, Shinobu; Furuoka, Hidefumi; Matsui, Takane; アグリプリヨノ, デヴィ　ラテイ; 中川, 迪夫; 両角, 徹雄; 吉原, 忍; 古岡, 秀文; 松井, 高峯.
Palavras-chave: Adenovirus; Encephalitis; Inclusion body; Nephritis; Swine.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1326