Consulta: subjectFacets:"Inhibitor"
Registros recuperados: 8
Data/hora: 11/06/2024 11:09:11

Pharmacological characterization of rat paw edema induced by Naja haje arabica venom
Provedor de dados: 80
Autores: Al-Asmari,A. K.
This investigation was performed in order to assess the inflammatory response induced by Naja haje arabica venom (NhaV) in rat hind paw. The inflammatory response was estimated by measuring the edema with a Plethysmometer. The venom (0.625-10mug/paw) produced a dose and time-dependent increase in non-hemorrhagic paw edema. The response to NhaV was maximal within 15 min and disappeared in 24 h. Five mug/paw of NhaV was chosen to test the effect of various drugs on the edema induced by this venom. Quinacrine (QNC), a phospholipase A2 (PLA2) inhibitor, and dipyridamole (DPM), an adenosine transport inhibitor, attenuated venom-induced edema in rat paw (P&lt;0.001). Commercially available antivenom was ineffective when administered intravenously, whereas its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat paw edema; Naja haje arabica venom; Inflammatory mediators; Inhibitor; Antivenom.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000100007

Babesia bovis: Effects of cysteine protease inhibitors on in vitro growth
Provedor de dados: 19
Autores: Okubo, Kazuhiro; Yokoyama, Naoaki; Govind, Yadav; Alhassan, Andy; Igarashi, Ikuo.
In the present study, we examined the effects of four kinds of cysteine protease inhibitors (E64, E64d, leupeptin, and ALLN) on the in vitro asexual growth of Babesia bovis. Of these, only the lipophilic inhibitors, E64d and ALLN, were found to effectively inhibit the growth of B. bovis. In further experiments, E64d, but not ALLN, significantly suppressed the parasite’s invasion of host erythrocytes, while both chemicals, especially ALLN, inhibited the parasite’s replication within the infected erythrocytes. These data suggested the presence of cysteine protease(s) derived from B. bovis, in which the protease(s) would play important roles in the erythrocyte invasion and/or replication processes of the parasite.
Palavras-chave: Babesia bovis; Cysteine protease; Inhibitor; Invasion; Replication.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1041

Assessment of natural vegetal extracts in the inhibition of Leuconostoc mesenteroides MSI
Provedor de dados: 114
Autores: Serra,M. A; Garnero,J. A; Nicolau,V. V; Andreatta,A. E.
The present study focuses on the assessment of natural vegetal extracts in the inhibition of bacteria altering Vienna sausages whose growth becomes evident in the vacuum packed product after two to three weeks of refrigerated storage causing packages to swell. The different natural extracts were obtained by means of steam stripping, microwave assisted extraction and ultrasound assisted extraction. Apart from these extracts, commercial essential oils, pure compounds present in the vegetal kingdom and solutions were also evaluated. For each of the extracts, microbial sensitivity tests were carried out in order to assess the inhibitory/bactericidal effect against Leuconostoc mesenteroides MS1. It has been found that the essential oils obtained from Citrus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leuconostoc mesenteroides MS1; Antimicrobial; Essential oil; Inhibitor; Bactericide; Vienna sausage.
Ano: 2018
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2018000100006

Schistosome serine protease inhibitors: parasite defense or homeostasis?
Provedor de dados: 42
Autores: Quezada,Landys A. Lopez; McKerrow,James H.
Serpins are a structurally conserved family of macromolecular inhibitors found in numerous biological systems. The completion and annotation of the genomes of Schistosoma mansoni and Schistosoma japonicum has enabled the identification by phylogenetic analysis of two major serpin clades. S. mansoni shows a greater multiplicity of serpin genes, perhaps reflecting adaptation to infection of a human host. Putative targets of schistosome serpins can be predicted from the sequence of the reactive center loop (RCL). Schistosome serpins may play important roles in both post-translational regulation of schistosome-derived proteases, as well as parasite defense mechanisms against the action of host proteases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosomiasis; Inhibitor; Protease; Serpin; Genome.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200025

Aurapten, a coumarin with growth inhibition against Leishmania major promastigotes
Provedor de dados: 56
Autores: Napolitano,H.B.; Silva,M.; Ellena,J.; Rodrigues,B.D.G.; Almeida,A.L.C.; Vieira,P.C.; Oliva,G.; Thiemann,O.H.
Several natural compounds have been identified for the treatment of leishmaniasis. Among them are some alkaloids, chalcones, lactones, tetralones, and saponins. The new compound reported here, 7-geranyloxycoumarin, called aurapten, belongs to the chemical class of the coumarins and has a molecular weight of 298.37. The compund was extracted from the Rutaceae species Esenbeckia febrifuga and was purified from a hexane extract starting from 407.7 g of dried leaves and followed by four silica gel chromatographic fractionation steps using different solvents as the mobile phase. The resulting compound (47 mg) of shows significant growth inhibition with an LD50 of 30 µM against the tropical parasite Leishmania major, which causes severe clinical manifestations...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coumarin; Aurapten (7-geranyloxycoumarin); X-rays; Leishmania; Inhibitor.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200010

Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells
Provedor de dados: 56
Autores: Ji,C.; Ren,F.; Xu,M.
Nasopharyngeal carcinoma is a common malignancy in Southern China of uncertain etiologic origin. Diallyl trisulfide (DATS), one of the major components of garlic (Allium sativum), is highly bactericidal and fungicidal. In this study, we investigated the function of p38 mitogen-activated protein kinase (MAPK) and caspase-8 in DATS-induced apoptosis of human CNE2 cells using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], flow cytometry assay, and Western blotting. After CNE2 cells were treated with DATS (50, 100, or 150 μM) for 24 h, cell viability rates were 75.9, 63.4 and 39.6%, and apoptosis rates were 24.5, 36.9, and 62.4%, respectively. The data showed that DATS induced CNE2 cell death in a dose-dependent manner. After human CNE2...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DATS; P38 MAPK; Caspase-8; Inhibitor; Apoptosis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900003

Detection of trypsin inhibitor in seeds of Eucalyptus urophylla and its influence on the in vitro growth of the fungi Pisolithus tinctorius and Rhizoctonia solani
Provedor de dados: 58
Autores: Tremacoldi,Célia Regina; Pascholati,Sérgio Florentino.
Inhibitors of plant proteases can regulate the hydrolysis of proteins inside the cells and also participate in the mechanisms of plant defense against herbivore insects and pathogens. Here, we demonstrated that seeds of Eucalyptus urophylla exhibit activities of trypsin and papain inhibitors, two proteases commonly found in living cells. Low amounts of proteins of the crude protein extract of seeds and fractions partially purified by gel filtration, with inhibitory activity against trypsin, inhibited in vitro the mycelial growth of a compatible isolate of the ectomycorrhizal fungus Pisolithus tinctorius and allowed an unsatisfactory growth of another isolate from Pinus taeda, considered incompatible for this eucalyptus species. The same amounts of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Protease; Inhibitor; Trypsin; Pisolithus tinctorius; Rhizoctonia solani.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400001

Biotransformation of bromhexine by Cunninghamella elegans, C. echinulata and C. blakesleeana
Provedor de dados: 58
Autores: Dube,Aman K.; Kumar,Maushmi S.
Abstract Fungi is a well-known model used to study drug metabolism and its production in in vitro condition. We aim to screen the most efficient strain of Cunninghamella sp. among C. elegans, C. echinulata and C. blakesleeana for bromhexine metabolites production. We characterized the metabolites produced using various analytical tools and compared them with mammalian metabolites in Rat liver microsomes (RLM). The metabolites were collected by two-stage fermentation of bromhexine with different strains of Cunninghamella sp. followed by extraction. Analysis was done by thin layer chromatography, high performance thin layer chromatography, Fourier transform infrared spectroscopy, high performance liquid chromatography and Liquid chromatography–mass...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cunninghamella sp.; Bromhexine; RLM; CYP3A4; Inhibitor; Metabolism.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200259