Consulta: subjectFacets:"Isoenzyme"
Registros recuperados: 6
Data/hora: 09/06/2024 14:55:52

Acid phosphatase polymorphism within and among populations of Cauliflower (Brassica oleracea var botrytis)
Provedor de dados: 74
Autores: Marino,Celso Luis; Gimenes,Marcos Aparecido; Silva,Norberto da; Lopes,Catalina Romero.
Eighty-one lines of cauliflower (Brassica oleracea var. botrytis) from 12 populations used to produce commercial hybrids in Brazil were screened for polymorphism in the acid phosphatase system, in order to evaluate the usefulness of this marker for the determination of the parental contamination level in hybrid seeds. Little polymorphism was detected in the examined lines, but the system appeared to be very useful for hybrid identification, since the only condition required was polymorphism between the two parental lines. If the analyzed lines were used for hybrid production, 8.4% and 12.3% of the possible crosses would result in hybrids which can be positively identified using the APS-1 and B1 loci, respectively. If only one plant of each homozygous type...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Polymorphism; Cauliflower.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100015

Isoenzymatic polymorphism in the leaf-cutting ant Atta capiguara Gonçalves (hymenoptera: formicidae)
Provedor de dados: 82
Autores: Cantagalli,Liriana B; Mangolin,Claudete A; Ruvolo-Takasusuki,Maria C C.
This study was carried out to analyze the genetic population structure of Atta capiguara from 12 nests collected in Tapejara in the state of Paraná, Brazil, using isoenzyme polymorphisms. The analyzed isoenzymes were esterases (EST - EC 3.1.1.1), acid phosphatase (ACP - EC 3.1.3.2) and carbonic anhydrase (CA - EC 4.2.1.1). Ten loci were found in A.capiguara and four polymorphic loci were detected. The observed heterozigosity (0.0296) was low when compared to the expected heterozigosity (0.1461). The high value of F IS (0.7954) shows an excess of homozygous genotypes probably caused by inbreeding.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isoenzyme; Genetic variability; Population genetics.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000100007

Comparative Biochemical and Restriction Endonuclease Digestion Pattern Analysis of Indian and Portland-1 Strains of Giardia lamblia
Provedor de dados: 19
Autores: Chaudhuri, P. P.; Sil, A. K.; Das, D.; Sengupta, K.; Bhattacharya, S.; Chattopadhyay, D. J.; Das, Pradeep.
Palavras-chave: Giardia; Isoenzyme; Restriction-endonuclease.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/202

Enzyme expression in indica and japonica rice cultivars under saline stress - doi: 10.4025/actascibiolsci.v34i4.8535
Provedor de dados: 3
Autores: Lima, Maria da Graça de Souza; Universidade Federal de Pelotas; Lopes, Nei Fernandes; Universidade Federal de Pelotas; Zimmer, Paulo Dejalma; Universidade Federal de Pelotas; Meneghello, Geri Eduardo; Universidade Federal de Pelotas; Mendes, Cristina Rodrigues; Universidade Federal de Pelotas; Amarante, Luciano do; Universidade Federal de Pelotas.
The southern State of Rio Grande do Sul (RS) is the main rice producer in Brazil with a 60% participation of the national production and 86% participation of the region. Rice culture irrigation system is done by flooding, which leads to soil salinization, a major environmental constraint to production since it alters the plants’ metabolism exposed to this type of stress. The indica cultivar, widely used in RS, has a higher sensitivity to salinity when compared to that of the japonica cultivar in other physiological aspects. Current research analyzes enzymes expression involved in salt-subjected indica and japonica rice cultivars’ respiration. Oryza sativa L. spp. japonica S.Kato (BRS Bojuru, IAS 12-9 Formosa and Goyakuman) and Oryza sativa L. spp. indica...
Palavras-chave: 2.00.00.00-6; 2.03.03.00-9; 2.08.00.00-2 electrophoresis; Isoenzyme; Krebs cycle; O. sativa L.; Salinity Fisiologia Vegetal.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8535

Isoenzyme and molecular approach for authenticating and monitoring of animal cell lines
Provedor de dados: 42
Autores: ARAÚJO,SHIRLEY B. DE; PATRICIO,GIANCARLO F.; SIMONI,ISABELA C.; RIVAS,ELIANA B.; FERNANDES,MARIA JUDITE B.
Abstract: Authentication of cell lines is of paramount importance to validate the results from their use in biomedical research. Although isoenzyme polymorphism is the standard method, molecular methods based on mitochondrial DNA (mtDNA) have been developed to replace it. The aim of this study was the improvement of our isoenzyme electrophoretic analysis and the validation of one molecular technique targeted at mtDNA for the authentication of our animal cell lines. The combined method of cellular lysing through osmotic shock, followed by freezing-thawing in N2 to obtain isoenzyme extracts, and with 42 × 106 cells maintained the best efficiency. The superior electrophoretic conditions were PAGE run at 200 V. All cell lines had isoenzymatic mobility...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Authentication; Cell line; DNA barcode; Isoenzyme.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500625

Genetic diversity in Egeria densa and E. najas in Jupiá Reservoir, Brazil
Provedor de dados: 118
Autores: Mori,Edson S; Martins,Dagoberto; Velini,Edivaldo D; Marino,Celso L; Gouvêa,Cantídio F; Leite,Suzi M.M; Camacho,Edwin; Guries,Raymond P.
The aquatic plant species Egeria densa (Planch.) Casp and E. najas Planchon occur naturally in the Paraná River Basin of southeastern Brazil. Hydroelectric dam construction in the river basin has created several lakes, changing the ecology of the river and altering the population ecology of Egeria. Large, dense populations of Egeria now grow in Jupiá Lake and its tributaries, occasionally blocking hydroelectric turbines. This study is part of a larger project examining environmental changes associated with large dam projects; the research objective of this study was to assess patterns of genetic diversity in Egeria populations growing in Jupiá and Três Irmãos Lakes and their Paraná River tributaries using genetic markers. Forty-two plants of E. najas and...
Tipo: Journal article
Palavras-chave: Egeria; Dam; Isoenzyme; Genetic distance; RAPD; Reservoir.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200008