Consulta: subjectFacets:"Isolation"
Registros recuperados: 56
Data/hora: 06/06/2024 16:22:41

Aislamiento y caracterización de Streptococcus bovis y estudio inicial para el aislamiento de su bacteriófago
Provedor de dados: 32
Autores: López Garrido, Serafín Jacobo.
El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal  Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta  alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa  (producción de AGV y lactato) y el proceso de liofilizado como método de  conservación, y se realizaron ensayos para expresar su bacteriófago. Para el  aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado  bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción  catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se  comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria  tiene forma esférica a ovoide, de 0.8...
Tipo: Tesis
Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage.
Ano: 2008
URL: http://hdl.handle.net/10521/1402

The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields
Provedor de dados: 69
Autores: Nunkaew,Tomorn; Kantachote,Duangporn; Nitoda,Teruhiko; Kanzaki,Hiroshi.
The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw...
Tipo: Journal article
Palavras-chave: Biofertilizer; Isolation; Paddy field; Purple nonsulfur bacteria; Rice straw broth.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007

Isolation of simple sequence repeats from groundnut
Provedor de dados: 69
Autores: Tang Wang,Chuan; Dao Yang,Xin; Xu Chen,Dian; Lin Yu,Shan; Zhen Liu,Guang; Yi Tang,Yue; Zhi Xu,Jian.
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
Tipo: Journal article
Palavras-chave: Groundnut; Isolation; Simple sequence repeat.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015

Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
Provedor de dados: 74
Autores: Coêlho,Mariza M.; Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Santos,André L.W.
Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Sequencing; Bacillus; Pantoea.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400021

Comunicação interpessoal em três assentamentos de reforma agrária de Unaí-Minas Gerais.
Provedor de dados: 14
Autores: ROCHA, F. E. de C.; ZOBY, J. L. F.; XAVIER, J. H. V.; GASTAL, M. L.
ABSTRACT: A research project on farming community development has been carried out by Embrapa Cerrados (Savannah Agricultural Research Center of the Brazilian Agricultural Research Corporation) in three Agrarian Reform Settlement Projects (PA): PA Jibóia, PA Paraíso and PA Santa Clara Furadinho, in Unaí country, Minas Gerais State, Brazil. At its initial phase, there was a diagnose and survey of information about the interpersonal interaction among group members. The objective of this article is to discuss the applicability of Moreno Sociometry in mapping the interpersonal relations between the land reform settlers to subsidize development of farming communities. initially, farmers were interviewed and requested to respond to three questions related to...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Relação interpessoal; Interação; Afinidade; Sociometria; Rural settlement; Agrarian reform; Communication; Small farmers; Interpessoal relation; Isolation; Sociometry; Affinity; Interaction; Analysis.; Análise; Assentamento; Comunicação; Isolamento; Liderança; Pequeno Produtor; Reforma Agrária.; Leadership.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/568179

Cultivating the uncultured: limits, advances and future challenges
Provedor de dados: 5
Autores: Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text
Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi
Provedor de dados: 5
Autores: Zappa, Sebastien; Rolland, Jean-luc; Flament, Didier; Gueguen, Yannick; Boudrant, Joseph; Dietrich, Jacques.
This work reports the first isolation and characterization of an alkaline phosphatase (AP) from a hyperthermophilic archaeon. An AP gene from Pyrococcus abyssi, a euryarchaeon isolated from a deep-sea hydrothermal vent, was cloned and the enzyme expressed in Escherichia coli. Analysis of the sequence showed conservation of the active site and structural elements of the E. coli AP. The recombinant AP was purified and characterized. Monomeric and homodimeric active forms were detected, with a monomer molecular mass of 54 kDa. Apparent optimum pH and temperature were estimated at 11.0 and 70 degreesC, respectively. Thus far, P. abyssi AP has been demonstrated to be the most thermostable AP, with half-lives at 100 and 105 degreesC of 18 and 5 h, respectively....
Tipo: Text
Palavras-chave: Escherichia coli; Characterization; Isolation; Gene; Alkaline phosphatase; Pyrococcus abyssi; Archaeon.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-1268.pdf

Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A 2 fromBothrops atrox snake venom
Provedor de dados: 80
Autores: Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.
Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337

An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
Provedor de dados: 80
Autores: Gómez,GN; Nerli,BB; Acosta,OC; Picó,GA; Leiva,LCA.
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Partition; Snake toxins; Isolation; Phospholipase A2; Proteases.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300008

Islands: Palaeontology, Geology and Tectonics
Provedor de dados: 16
Autores: Donovan, S.K.
The intention of this Lyell Meeting is to bring together tectonicists, geologists, palaeontologists and geomorphologists to discuss the intersection of their specialisms in the geology of islands.
Tipo: Article / Letter to the editor
Palavras-chave: Lyell meetings; Islands; Geology; Palaeontology; Tectonics; Evolution; Isolation; 38.20; 38.37; 38.22.
Ano: 2011
URL: http://www.repository.naturalis.nl/record/428038

Description of Neblinichthys brevibracchium and N. echinasus from the upper Mazaruni River, Guyana (Siluriformes: Loricariidae), and recognition of N. roraima and N. yaravi as distinct species
Provedor de dados: 83
Autores: Taphorn,Donald C.; Armbruster,Jonathan W.; López-Fernández,Hernán; Bernard,Calvin R.
Neblinichthys brevibracchium and N. echinasus are new sympatric species from upland tributaries of the Mazaruni River (Essequibo River basin) of Guyana. These two new species are the first Neblinichthys reported from Guyana. Adult males of both new species have short pectoral-fin spines and several series of hypertrophied odontodes covering the entire dorsal surface and along the entire edge of the snout. They are distinguished from each other by dorsal-fin base length/standard length (18.1-22.8% in N. brevibracchium vs. 24.3-27.0% in N. echinasus), dorsal-anal distance/SL (13.7-18.3% in N. brevibracchium vs. 11.9-12.5% in N. echinasus), by having the snout decreasing in steep arc just anterior to eyes and then flattening in area anterior of nares in N....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Essequibo; Guiana Shield; Isolation; Endemism; Punk Catfish.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000300006

Folyami hajózás és a magyarországi gabonaexport
Provedor de dados: 31
Autores: Szilvassy,   Zoltan.
Az árutermelésre áttért mezőgazdaság közel másfél évszázada alatt bebizonyosodott, hogy a megtermelt terményfelesleg az ország gazdaságföldrajzi „bezártsága” miatt nem versenyképes a tengeri kijutással rendelkező országok gabonájával szemben. Atengeri kikötőhöz vezető olcsó vízi utat máig sem sikerült megteremteni, és a magyar gabona jelenleg is csak a világpiaci árnál alacsonyabb áron értékesíthető. Afolyók vízjárástól független hajózhatósága azok belépcsőzésével valósítható meg.  -------------------------------------------  Over the one and a half centuries since agriculture shifted from self-sufficiency to producing marketable goods, it has been proven that the surplus produce – due to the country’s geographical isolation – cannot compete against the...
Tipo: Journal Article
Palavras-chave: Gazdaságföldrajz; Bezártság; Belvízi hajózás; Vízlépcsők; Eco-geography; Isolation; Inland navigation; Weirs; Agricultural and Food Policy; International Relations/Trade.
Ano: 2009
URL: http://purl.umn.edu/92479

METHOD OF DEFINING THE ISOLATION PARAMETERS IN 1000 WATT SYMMETRIC ELECTRICITY NETWORK
Provedor de dados: 31
Autores: Sidorov,   Alexander; Utegulov,   Bolatbek; Utegulov,   Arman; Begentaev,   Bagustar; Uahitova,   Aygul; Amurgaliev,   Samat.
The authors define parameters for isolation three-phase electric network to provide safety conditions for personnel working at open-operated coal deposits. The authors conclude the technique provides satisfactory accuracy of the parameters’ defining, as well as simplicity and safety of coal mining works with electric network having pressure up to 1000 Watt.
Tipo: Journal Article
Palavras-chave: Isolation; Electric network; Electric pressure; Isolated neutral; Research and Development/Tech Change/Emerging Technologies.
Ano: 2009
URL: http://purl.umn.edu/94561

Banana Supply Chains in Indonesia and Australia: Surviving Isolation from End markets
Provedor de dados: 31
Autores: Singgih,   Shinta; Woods,   Elizabeth.
Two banana supply chains (BSC), one in Indonesia (BSC1) and one in Australia (BSC2), are described based on case study research conducted in 2002. BSC1 sources bananas from small farmers for distribution through traditional markets in Jakarta. BSC2 includes farmers supplying to both major and independent retailers in Sydney through a wholesale agency with some farmer ownership. Farmers in both chains live in relative isolation from the main markets for their bananas. Prices for their bananas depend on the market prices in the city, upon which they have little control. This paper focuses on how farmers removed from key markets, deal with buyers to receive fair prices. In both chains, most transactions between farmers and their buyers were completed through...
Tipo: Conference Paper or Presentation
Palavras-chave: Farmers; Banana supply chain; Isolation; Trust.; Crop Production/Industries; Industrial Organization.
Ano: 2003
URL: http://purl.umn.edu/24336

Incidence of Salmonella in imported day-old ducklings. Brazil, 1998-2003
Provedor de dados: 89
Autores: Ribeiro,SAM; Galleti,MCM; Orsi,MA; Ferrati,AR; Mendonça,AO; Doretto Júnior,L; Camillo,SCA; Reischak,D.
The occurrence of Salmonella in a samples of 40 imported day-old duckling flocks was assessed from 1998 to 2003 according to the guidelines of the Brazilian National Poultry Health Program (Programa Nacional de Sanidade Avícola-PNSA). The pathogen was recovered from 26 flocks (65.0%). The most common serovars were S. Saintpaul and S. Kottbus. Up to four serovars were isolated from a single flock. Transportation box swabs (82.6%) and yolk sac pool (47.1%) showed the highest and the lowest frequency of Salmonella isolation, respectively. The high percentage of Salmonella isolation from imported day-old ducklings causes concern because of the zoonotic potential of this agent and its economical importance to commercial poultry breeding.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella; Imported ducklings; Isolation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100006

Newcastle disease virus infection in sparrows (Passer domesticus, Linneaus, 1758) captured in poultry farms of the agreste region of the State of Pernambuco
Provedor de dados: 89
Autores: Silva,JSA; Mota,RA; Vilela,SMO; Doretto Júnior,L; Pinheiro Júnior,JW; Silva,LBG.
Reservoir competence for the Newcastle Disease virus (NDV) was evaluated in sparrows (Passer domesticus, Linnaeus 1758) captured on a commercial poultry farm and a chicken hatchery in the State of Pernambuco, Northeastern Brazil. A total number of 103 birds collected from a poultry farm (24/103) and a chicken hatchery (79/103) were examined. Hemagglutination inhibition tests, isolation, and viral characterization were performed in all samples collected from each bird. Titers ranging from 1:2 to 1:64 were detectable in 10.68% of sparrows, but positive serology and viral isolation were obtained only from sparrows captured at the hatchery. Hemagglutination activity was inhibited by anti-avian paramyxovirus serotype 1 (APMV-1) serum, and this sample showed an...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Newcastle Disease; Sparrows; Reservoir; Isolation; Serology (HI); Poultry farm; Lentogenic strains (B1).
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000200009

Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.
Provedor de dados: 89
Autores: Rodriguez,JM; Rondón,IS; Verjan,N.
ABSTRACT Salmonella enterica is a large group of Gram-negative bacteria responsible for a number of foodborne infections associated with the consumption of contaminated poultry products. The hygienic status of raw chicken meat marketed at Ibague, Tolima, Colombia, is currently unknown. To address this issue, a cross-sectional study was conducted to estimate the prevalence of Salmonella spp., in raw chicken marketed at different outlets in this city. Salmonella spp. was isolated by standard microbiological methods, followed by biochemical, serological, and molecular confirmation. Additionally, risk factors associated with the presence of the bacteria were identified. The prevalence of Salmonella in raw chicken was 17.41% (47/270), and 14 different serotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Poultry; Prevalence; Risk factors; Serotyping.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400545

Rotavirus detection and isolation from chickens with or without symptoms
Provedor de dados: 89
Autores: Villarreal,LYB; Uliana,G; Valenzuela,C; Chacón,JLV; Saidenberg,ABS; Sanches,AA; Brandão,PE; Jerez,JA; Ferreira,AJP.
Rotaviruses have been identified as one of the main etiological agents of diarrhea and enteritis in mammals, including humans, and in avian species. Few studies have been published about enteric viruses in Brazilian poultry, including those related to rotavirus infection. Such studies demonstrate significant occurrence and the importance of enteric viruses in poultry presenting intestinal problems. Enteric viruses are the primary cause of injuries to the gut, allowing other agents, especially bacteria, to attach, to penetrate, and to replicate in the enteric tissue, leading to further damage. The aim of the present study was to detect rotavirus in the intestinal contents of layers and broilers by polyacrylamide gel electrophoresis (PAGE) and virus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteric viruses; Rotavirus; Isolation; MA104 cells; Chicken.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000300009

Sharing of termites (Blattodea: Isoptera) between sugarcane matrices and Atlantic Forest fragments in Northeast Brazil
Provedor de dados: 92
Autores: Couto,Alane Ayana Vieira de Oliveira; Montes,Martín Alejandro; Chaves,Rozzanna Esther Cavalcanti Reis de Figueirêdo; Vasconcellos,Alexandre.
Abstract The Atlantic Forest of South America is one of the most degraded tropical forests and the cultivation of sugarcane is considered one of the main causes. In humid forests termites stand out with regard to their abundance and functional importance. The present study aimed to compare termite assemblages of fragments of the Atlantic Forest with that of the sugarcane matrices that surround them. Collections were performed in two sugarcane plantations in Northeast Brazil. In each plantation a fragment of Atlantic Forest and an adjacent sugarcane field were sampled using a standardized termite sampling protocol. A total of 39 species and 302 encounters were recorded. Species richness, relative abundance and composition differed significantly between...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Conservation; Feeding groups; Isolation; Species dispersal; Species richness.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000200108

A reassessment of the phytogeographic characterization of Temperate Pacific South America
Provedor de dados: 97
Autores: SANTELICES,BERNABE; MENESES,ISABEL.
In 1980, a study of geographic distribution patterns and geographic affinities of benthic algae of temperate Pacific South America (5-55 ºS) provided a first characterization of these coasts. High endemism and limited floristic exchange with the Tropical Pacific and with islands in the South Pacific at various distances from the continent were two outstanding features of the marine flora. A partial blockage of species exchange partially accounted for a peculiar latitudinal pattern of species richness that increases with increasing latitudes. In contrast, there was reduced species richness in comparison with other climatically equivalent regions that have contact with more effective routes of migration. Over the last two decades, more than 30 taxonomic and...
Tipo: Journal article
Palavras-chave: Benthic phytogeography; Isolation; Latitudinal patterns; Seaweeds; Temperate South America.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2000000400005

Primeiro isolamento de Actinobacillus seminis em um caprino no município de Patos, Paraíba, Brasil
Provedor de dados: 195
Autores: Santos, Fabrine A.; Azevedo, Sergio S.; Mota, Rinaldo Aparecido; Kim, Pommy C.P.; Gomes, A. L. V.; Alves, Clebert José.
É relatado o primeiro isolamento de Actinobacillus seminis em um caprino no município de Patos, Estado da Paraíba, região semiárida do Brasil. Em um rebanho com 70 caprinos e 65 ovinos criados juntos, um reprodutor da espécie caprina da raça Moxotó, com quatro anos de idade, apresentou um quadro clínico de orquite e epididimite unilateral com consistência firme. O diagnóstico da infecção por A. seminis foi confirmado pela associação dos achados clínicos, isolamento bacteriano e, PCR e sequenciamento do gene 16S do rRNA. O resultado obtido sugere que A. seminis pode ser uma importante causa de infertilidade em caprinos da região.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation.
Ano: 2015
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25222

Isolation and Analysis of Recombinant EPSP synthases from Microbial Pathogens for Drug Design Purpose
Provedor de dados: 106
Autores: Vaithanomsat, P.; Brown, K.
Palavras-chave: Isolation; Analysis; Recombinant.
Ano: 1461
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5025

ISOLATION OF THE HUMAN CYTOMEGALOVIRUS FROM BODILY FLUIDS
Provedor de dados: 37
Autores: CAMACHO ORTEGA,Sigrid Johanna; BOHORQUEZ ÁVILA,Sonia del Pilar; VELANDIA ROMERO,Myriam Lucia; CASTELLANOS PARRA,Jaime Eduardo.
ABSTRACT In vitro studies on the pathogenesis of the human cytomegalovirus (HCMV) are conducted regularly using laboratory adapted strains that lose some characteristics during the adaptation process. Since HCMV is excreted from bodily fluids during infection or reactivation, this work aimed to isolate and culture HCMV from the MRC-5 human cells found in the urine, bronchoalveolar lavage, saliva, and plasma samples of pediatric patients with probable or confirmed infection. The samples were inoculated on cell cultures either for 14 days or until a cytopathic effect (CPE) of 80 % was observed. The cell lysates and supernatants were used to perform successive viral passages. Besides HCMV, the herpes simplex virus was detected from all the saliva samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Body fluids; Cell culture; Cytomegalovirus; Isolation.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300520

CELL CULTURE ISOLATION OF HEPATITIS E VIRUS GENOTYPE 3 STRAIN OBTAINED FROM HUMAN FECES
Provedor de dados: 37
Autores: QUINTERO-GIL,Carolina; MIRAZO,Santiago; PARRA-SUESCÚN,Jaime; LÓPEZ-HERRERA,Albeiro; MAINARDI,Victoria; ARBIZA,Juan; ORDÚZ,Sergio.
ABSTRACT Hepatitis E virus (HEV) is considered one of the leading causes of acute viral hepatitis worldwide, and about 20 million infections and approximately 57 000 deaths occurred every year. However, little is known about the replicative virus cycle due to the absence of a consensus cell culture model. A549 cell line is considered susceptible to HEV genotype 3, however, both viral strain and cell culture conditions could affect the viral isolation in vitro. The objective of this work was to isolate in vitro an HEV-3 strain obtained from human feces. To this, a genotype 3 HEV strain previously identified by genetic characterization was inoculated in A549 monolayers, and incubated for two hours at 37 °C. Five days post-infection, cells were passaged...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A549 cells; Hepevirus; Isolation; RNA virus.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300503

Isolation of Salmonella enterica subsp. enterica (O:4,5:i) and Salmonella enterica subsp. Typhimurium from free-living domestic pigeons (Columba livia)
Provedor de dados: 45
Autores: Rocha-e-Silva,R.C.; Cardoso,W.M.; Teixeira,R.S.C.; Albuquerque,Á.H.; Horn,R.V.; Lopes,E.S.; Gomes Filho,V.J.R.; Almeida,C.P.; Santos,I.C.L.; Machado,D.N.; Lima,S.V.G.; Carneiro,I.S.
The present study reports the isolation of Salmonellaenterica in organs of free-living domestic pigeons. In the clinic examination, the presence of feces in the peri-cloacal and abdominal regions were observed, as well as symptoms such as cachexy, incoordination and opisthotonos. Before any therapeutic protocol was applied the bird died and a necropsy was then performed for the removal of spleen, liver, kidney and intestine for bacteriological examination and antibiotic sensitivity test. Salmonella enterica subsp.enterica (O:4,5:i-) and Salmonellaenterica subsp. enterica serovar Typhimurium were isolated from the liver and intestine and the sensitivity test demonstrated that these strains are sensitive to several antibiotics.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Necropsy; Bacteriological examination; Sensitivity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501435

Isolation and genetic stability of an infectious bronchitis virus strain (IBV)
Provedor de dados: 45
Autores: Pereira,C.G.; Saraiva,G.L.; Santos,M.R.; Assao,V.S.; Fietto,J.L.R.; Bressan,G.C.; Almeida,M.R.; Moreira,M.A.S.; Silva Júnior,A.
RESUMO O vírus da bronquite infecciosa (IBV) é um importante patógeno respiratório presente na avicultura comercial e tem provocado grandes perdas econômicas em todo o mundo. A vacinação é realizada pela indústria produtora de aves, mas continuam surgindo novos sorotipos e variações antigênicas, dificultando o controle de IBV. Nós realizamos uma caracterização molecular de uma cepa de IBV obtida diretamente de tecidos e comparamos com a mesma cepa que havia sido passada três vezes em ovo embrionado. Nós mostramos uma variação significante na sequência viral depois de ter sido isolada em ovo embrionado.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Infectious bronchitis virus; Isolation; Characterization; Stability.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401333

Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Provedor de dados: 45
Autores: Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M.
The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monkey; Preantral follicle; Isolation; Ovary; Cebus apella.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009

Isolation and biological characterization of a basic phospholipase A2 from Bothrops jararacussu snake venom
Provedor de dados: 47
Autores: Maruñak,S.L.; Leiva,L; Garcia Denegri,M.E.; Teibler,P; Acosta De Pérez,O.
A phospholipase A2 has been isolated from Bothrops jararacussu venom from snakes that inhabit the northeast region of Argentina. The present study describes in vivo and in vitro biological activities of phospholipase A2 from B. jararacussu as well as isolation details of its. Venom was obtained by milking of adult snakes which were housing in wood reptile cages of varying dimensions in heated (20-30ºC) rooms. Snakes received a weekly diet of mice and water was available ad libitum for drinking and soaking. The enzyme was purified by gel filtration on a Sephadex G-75 column followed by ion exchange chromatography on a SP-Sephadex C25 column. The major peak belonging to proteins was retained in the cation exchanger and then eluted using a concentration...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Snake venom; Bothrops jararacussu; Phospholipase A2; Isolation.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300001

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview
Provedor de dados: 52
Autores: Fathi,Ezzatollah; Farahzadi,Raheleh.
ABSTRACT The aim of this review was to describe the current state-of-the-art regarding isolation, characterization and aging of adipose tissue-derived mesenchymal stem cells (ADSCs). Mesenchymal stem cells (MSCs) have recently received widespread attention because of their potential use in tissue-engineering applications. Various studies have indicated that MSCs with a fibroblast-like morphology migrate to the sites of injury and help to regenerate damaged tissue. Over the past few years, it has been recognized that fat is not only an energy supply, but also a rich source of multipotent stem cells that can be easily harvested, isolated and selected as compared with other tissues. ADSCs are particularly interesting because of their rapid proliferation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adipose tissue-derived mesenchymal stem cells; Adipose tissue; Isolation; Aging; Differentiation potential.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100302

Optimization of fermentation conditions for cellulases production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from Indian hot spring
Provedor de dados: 52
Autores: Acharya,Somen; Chaudhary,Anita.
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55°C, respectively. Maximum cellulases production by both the isolates was detected after 60 h incubation period using wheat and rice straw. The combination of inorganic and organic nitrogen source was suitable for cellulases production. Overall, FPase production was much higher than CMCase production by both of the strains. Between the two thermophiles, the cellulolytic activity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Thermophiles; Submerged fermentation; Parameter optimization; Cellulases; Hot spring.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400003

Social condition affects hormone secretion and exploratory behavior in rats
Provedor de dados: 56
Autores: Genaro,G.; Schmidek,W.R.; Franci,C.R.
Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008

Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase
Provedor de dados: 56
Autores: Ren,H.J.; Zhang,C.L.; Liu,R.D.; Li,N.; Li,X.G.; Xue,H.K.; Guo,Y.; Wang,Z.Q.; Cui,J.; Ming,L.
The epithelium is a highly dynamic system, which plays a crucial role in the homeostasis of the intestinal tract. However, studies on the physiological and pathophysiological functions of intestinal epithelial cells (IECs) have been hampered due to lack of normal epithelial cell models. In the present study, we established a reproducible method for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (≤20 min). With this method, continuously growing mouse IECs, which can be subcultured over a number of passages, were obtained. The obtained cell lines formed a tight cobblestone-like arrangement, displayed long and...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Intestinal epithelial cells; Crypt; Isolation; Primary culture; Collagenase I; Hyaluronidase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000500604

Thermostable chitinase from Cohnella sp. A01: isolation and product optimization
Provedor de dados: 58
Autores: Aliabadi,Nasrin; Aminzadeh,Saeed; Karkhane,Ali Asghar; Haghbeen,Kamahldin.
Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biochemical characterization; Chitinase; Cohnella sp. A01; Isolation; Thermostable.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400931

Isolation of rabies virus from the parotid salivary glands of foxes (Pseudalopex vetulus) from Paraíba State, Northeastern Brazil
Provedor de dados: 58
Autores: Silva,Maria Luana Cristiny Rodrigues; Lima,Fabiano da Silva; Gomes,Albério Antônio de Barros; Azevedo,Sérgio Santos de; Alves,Clebert José; Bernardi,Fernanda; Ito,Fumio Honma.
To determine the presence of rabies virus in the parotid salivary glands, 12 road-killed rabies-positive hoary foxes (Pseudoalopex vetulus) were tested by using the fluorescent antibody test (FAT) and mouse inoculation test (MIT). All 12 parotid salivary glands were positive for both tests, although in some cases several passages were required. The findings of this study support the importance of the hoary fox as rabies reservoir in the semi-arid region of Paraíba State, Northeastern Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parotid gland; Fox; Rabies virus; Isolation; Paraíba State.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300004

Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Provedor de dados: 58
Autores: Puentes,R; Eliopulos,N; Pérez,R; Franco,G; Sosa,K; Bianchi,P; Furtado,A; Hübner,S.O.; Esteves,P.A.
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Canine Parvovirus 2c; Isolation; Molecular characterization; South America.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300022

Arcobacter butzleri: first isolation report from chicken carcasses in costa rica
Provedor de dados: 58
Autores: Arias,Maria Laura; Cid,Adriana; Fernandéz,Heriberto.
Arcobacter butzleri isolation from chicken carcasses in Costa Rica is reported for the first time. The isolated strains (P and R) were presumptively identified by their phenotypic characteristics. Definitive identification was made using a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. These first isolations indicate the necessity of further investigation about the prevalence, distribution, ecology and interactions with human beings of this and other Arcobacter species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arcobacter butzleri; Chicken carcasses; Isolation; Epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200035

Comparative study of Mycobacterium bovis primary isolation methods
Provedor de dados: 58
Autores: Issa,Marina de Azevedo; Soares Filho,Paulo Martins; Fonseca Júnior,Antônio Augusto; Hodon,Mikael Arrais; Santos,Lílian Cristian dos; Reis,Jenner Karlisson Pimenta dos; Cerqueira Leite,Rômulo.
Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement “A” (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared. Regarding the isolation, there were no significant differences...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium bovis; Diagnosis; Middlebrook 7H11; Isolation; Sulphuric acid.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100139

Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil
Provedor de dados: 58
Autores: Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano.
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016

Isolation of Actinobacillus seminis from a goat with clinical epididymo-orchitis in Brazil
Provedor de dados: 58
Autores: Santos,Fabrine Alexandre dos; Azevedo,Edísio Oliveira de; Azevedo,Sérgio Santos de; Garino Júnior,Felício; Mota,Rinaldo Aparecido; Kim,Pomy de Cássia Peixoto; Gomes,Ana Lisa Vale; Alves,Clebert José.
The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100027

Curtobacterium flaccumfaciens pv. flaccumfaciens detection in bean seeds using a semi-selective culture medium
Provedor de dados: 58
Autores: Maringoni,Antonio Carlos; Camara,Renata de Cassia.
The bacterial wilt caused by Curtobacterium flaccumfaciens pv. flaccumfaciens is currently considered one of the most important bacterial bean disease in Brazil. One of the most effective control methods against this disease is the use of healthy seeds. However, no methods are known that could be routinely used to detect this bacterium in bean seeds under Brazilian condition. The aim of this work was to evaluate qualitative and quantitative detection methods for Curtobacterium flaccumfaciens pv. flaccumfaciens in naturally-infected bean seeds, and the detection of this pathogen in thirty bean seed samples, by sowing onto a semi-selective culture medium the leachate obtained from soaked bean seeds. Both the qualitative and quantitative methods were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterial wilt; Isolation; Seed pathology; Phaeolus vulgaris.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400009

Isolation, properties and kinetics of growth of a thermophilic Bacillus
Provedor de dados: 58
Autores: Souza,Adriane Nunes de; Martins,Meire Lelis Leal.
A strictly aerobic, thermophilic, spore-forming bacteria was isolated from a soil sample collected in Campos dos Goytacazes City, Rio de Janeiro, Brazil. The cells of this organism were Gram-positive, catalase positive, actively motile, 1.2 µm wide and 5.3 µm long. Growth occurred at pH values ranging from 6.5 to 9.0, and optimum growth occurred at about pH 7.0. The optimum temperature for growth was around 55ºC, and the upper temperature limit for growth was around 70ºC. Yeast extract stimulated growth on glucose and was obligately required at supraoptimal temperatures (65ºC). The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1, and these three organisms...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thermophilic microorganism; Bacillus; Isolation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400003

A novel acidophilic, thermophilic iron and sulfur-oxidizing archaeon isolated from a hot spring of tengchong, yunnan, China
Provedor de dados: 58
Autores: Ding,Jiannan; Zhang,Ruiyong; Yu,Yizun; Jin,Decai; Liang,Changli; Yi,Yang; Zhu,Wei; Xia,Jinlan.
A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm×1.0-1.31 µm in the medium containing elemental sulfur and 1.5-2.22 µm×1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65) and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor. Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor. Results of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Identification; Acidianus manzaensis; Bioleaching.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200016

EMJH medium with 5-fluorouracil and nalidixic acid associated with serial dilution technique used to recover Leptospira spp from experimentally contaminated bovine semen
Provedor de dados: 58
Autores: Miraglia,Fabiana; Moraes,Zenaide Maria de; Melville,Priscilla Anne; Dias,Ricardo Augusto; Vasconcellos,Silvio Arruda.
Bovine semen experimentally contaminated with Leptospira santarosai serovar Guaricura was submitted to the modified EMJH medium with 5-fluorouracil (300mg/L) and nalidixic acid (20mg/L), named as "selective medium" and using the serial dilution technique, in order to evaluate the percentage of recovery of the added microorganism. The selective EMJH medium was found with higher percentage of recovery of leptospiras and minor losses of samples due to contamination with opportunistic microorganisms than the non-selective EMJH medium: 151/376 (40.0%) of positive growth; and 38/376 (10.0%) contamination and 58/376 (15%) and 129/376 (34.0%), respectively. These results were statistically significant (p&lt;0. 0001; Fisher). Differences were found when the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospires; Culture media; Antimicrobial; Isolation; Semen.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100033

Comparison of methods for mycobacteria isolation from swine feces
Provedor de dados: 58
Autores: Oliveira,Eugenia Márcia de Deus; Rodriguez,César Alejandro Rosales; Rocha,Vivianne Cambuí Mesquita; Ambrosio,Simone Rodriguez; Ohara,Patrícia Miyuki; Amaku,Marcos; Ferreira,Fernando; Dias,Ricardo Augusto; Leão,Sylvia Cardoso; Ferreira Neto,José Soares.
Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium avium; Isolation; Feces; Swine.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019

Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells
Provedor de dados: 58
Autores: Jerez,José Antonio; Gregori,Fábio; Brandão,Paulo Eduardo; Rodriguez,Cesar Alejandro Rosales; Ito,Fumio Honma; Buzinaro,Maria da Glória; Sakai,Takeo.
Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine coronavirus (BCoV); Isolation; HmLu-1 cells.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001

Isolation and survival of Yersinia enterocolitica in ice cream at different pH values, stored at -18°c
Provedor de dados: 58
Autores: Pederiva,Norma B. Barbini de; Guzmán,Ana M. Stefanini de.
The presence of Yersinia enterocolitica was investigated in 203 samples of industrial (123) and non-industrial ice cream (80). Two Y. enterocolitica strains were isolated from non-industrial ice cream, which suggests the possibility of post-manufacturing contamination. One strain was typed as B:1A, O: 3,50,51; lis Xz, while the other one was biotyped as: B:1A but not serologically typed. Survival of Y. enterocolitica was investigated by inoculating nine samples of industrially manufactured ice cream to contain 20 CFU/ml of Y. enterocolitica and stored at -18°C for 480 days. The inoculated samples were classified into three different groups according to their pH (Group 1: pH 4-5; Group 2: pH 5-6 and Group 3: pH 6-7). Viability was determined by a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yersinia; Isolation; Survival; Ice cream; PH.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300005

Brucella spp. isolation from dogs from commercial breeding kennels in São Paulo state, Brazil
Provedor de dados: 58
Autores: Keid,Lara B.; Soares,Rodrigo M.; Morais,Zenaide M.; Richtzenhain,Leonardo J.; Vasconcellos,Sílvio A.
Dogs from 12 commercial breeding kennels were submitted to clinical investigation and laboratorial tests for diagnosis of Brucella spp. infection. The sampling was carried out between April 2000 and February 2002 and the laboratorial tests employed were agar gel immunediffusion test (AGID) and blood culture. From 171 dogs examinated, 39 (22.8%) showed at least one clinical sign compatible with brucellosis, 58 (33.91%) were AGID positive and 24 (14.03%) were positive by blood culture. Gram negative bacterial cells with a biochemical pattern compatible with that of bacteria belonging to genus Brucella were isolated from blood specimens of 24 animals. According to Kappa index and McNemar test, the association between AGID and blood culture (k=0.360 with 95%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dogs; Brucellosis; Brucella canis; Diagnosis; Isolation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100027

Isolation of Mucorales from processed maize (Zea mays L.) and screening for protease activity
Provedor de dados: 58
Autores: Santiago,André Luiz Cabral Monteiro de Azevedo; Motta,Cristina Maria de Souza.
Mucoraleswere isolated from maize flour, corn meal and cooked cornflakes using surface and depth plate methods. Rhizopus oryzae, Circinella muscae, Mucor subtilissimus,Mucor hiemalis f. hiemalis, Syncephalastrum racemosum, Rhizopus microsporus var. chinensis and Absidia cylindrospora showed protease activity.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zygomycetes; Food; Contamination; Isolation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400019

Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats
Provedor de dados: 58
Autores: Ferreira,Thais Sebastiana Porfida; Felizardo,Maria Roberta; Gobbi,Debora Dirani Sena de; Moreno,Marina; Moreno,Andrea Micke.
Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cat; Pasteurela multocida; Virulence genes; Resistance; Isolation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100271

Detection of seasonal asymptomatic dermatophytes in Van cats
Provedor de dados: 58
Autores: Ilhan,Ziya; Karaca,Mehmet; Ekin,Ismail Hakki; Solmaz,Hasan; Akkan,Hasan Altan; Tutuncu,Mehmet.
Abstract The Van cat is a domestic landrace found in the Van province of eastern Turkey. In this study, we aimed to determine the seasonal carriage of dermatophytes in Van cats without clinical lesions. A total of 264 hair specimens were collected from clinically healthy cats in and around the Van Province. Of these samples, 30.3% were obtained in spring, 30.6% in summer, 16.6% in autumn, and 22.3% in winter; 45.1% of samples were from male cats and the rest from female ones. Of the studied cats, 118 were younger than 1 year, 78 were 1–3 years old, and 68 were older than 3 years. The specimens were subjected to direct microscopic examination with 15% potassium hydroxide and cultured on Sabouraud dextrose agar and dermatophyte test medium supplemented with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carriage; Dermatophytes; Isolation; Van cat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100225

Comparative analysis of cultural isolation and PCR based assay for detection of Campylobacter jejuni in food and faecal samples
Provedor de dados: 58
Autores: Singh,Harkanwaldeep; Rathore,R.S; Singh,Satparkash; Cheema,Pawanjit Singh.
In the present study, the efficacy of polymerase chain reaction (PCR) based on mapA gene of C. jejuni was tested for detection of Campylobacter jejuni in naturally infected as well as spiked faecal and food samples of human and animal origin. Simultaneously, all the samples were subjected to the cultural isolation of organism and biochemical characterization. The positive samples resulted in the amplification of a DNA fragment of size ~589 bp in PCR assay whereas the absence of such amplicon in DNA extracted from E. coli, Listeria, Salmonella and Staphylococcus confirmed the specificity of the primers. Of randomly collected 143 faecal samples comprising human diarrheic stools (43), cattle diarrheic faeces (48) and poultry faecal swabs (52) only 4, 3 and 8,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Campylobacter jejuni; Isolation; PCR; Spiking.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100022

Detection of Mycoplasma pulmonis in laboratory rats
Provedor de dados: 58
Autores: Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.
This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015

The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of turkey
Provedor de dados: 58
Autores: Cihan,Arzu Coleri; Tekin,Nilgun; Ozcan,Birgul; Cokmus,Cumhur.
Previously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 %...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Isolation; Temperature requirement; Endospore-forming bacilli; 16S rRNA gene; ARDRA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100037

Isolation of Leptospira spp. from a man living in a rural area of the Municipality of Cruz Alta, RS, Brazil
Provedor de dados: 65
Autores: Silva,Felipe Jorge da; Silva,Glaucenyra Cecília Pinheiro; Loffler,Sylvia Grune; Brihuega,Bibiana; Samartino,Luis Ernesto; Alarcon,Miguel Frederico Fernandez; Santos,Carlos Eduardo Pereira dos; Mathias,Luis Antonio.
The aim of this study was to describe the isolation of a pathogenic strain of Leptospira interrogans from the urine sample of a male human living in the rural area of the County of Cruz Alta, Rio Grande do Sul. An aliquot of each urine sample was sown in a Fletcher and Ellinghausen - McCullough - Johnson - Harris (EMJH) media. Samples in which there was growth of spirochetes were sent to the Leptospirosis Laboratory of the Institute of Pathobiology in the National Institute of Agricultural Technology, Buenos Aires, Argentina and were typified by the Multiple Locus of Variable Number Tandem Repeat technique (MLVA). Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 was isolated, and this is a very important finding that serves as a warning to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human leptospirosis; Isolation; MLVA; Farm; Brazil.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100047

Leptospirosis in native mixed-breed sheep slaughtered in a semiarid region of Brazil
Provedor de dados: 65
Autores: Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José.
ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451

Isolation and identification of Mycobacterium bovis in milk from cows in northeastern Brazil
Provedor de dados: 65
Autores: Ramos,Joelson Marcolino; Heinemann,Marcos Bryan; Ferreira Neto,José Soares; Filho,Antônio Francisco de Souza; Cárdenas,Nicolás Céspedes; Alves,Clebert José; Azevedo,Sérgio Santos de.
ABSTRACT: Milk samples from 16 cows that tested positive on the tuberculin test in the state of Paraíba, northeastern Brazil, were used for mycobacteria isolation and identification. Mycobacteria were isolated from five (31.25%) of the 16 milk samples; three samples were classified as M. bovis, and two as belonging to the Mycobacterium genus. This is probably the first study of isolation and identification of M. bovis in milk from cows in Northeastern Brazil, which suggests that humans are at risk of contamination by ingestion.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium bovis; Isolation; Milk; PCR; Cattle.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202166