Consulta: subjectFacets:"Ixodes ricinus"
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Data/hora: 14/06/2024 01:56:56

Detection of Borrelia-specific 16S rRNA sequence in total RNA extracted from Ixodes ricinus ticks
Provedor de dados: 45
Autores: Radulović,Ž.; Milutinović,M.; Tomanović,S.; Mulenga,A.
A reverse transcriptase - polymerase chain reaction based assay for Borrelia species detection in ticks was developed. The method was based on amplification of 552 nucleotide bases long sequence of 16S rRNA, targeted by Borrelia specific primers. In the present study, total RNA extracted from Ixodes ricinus ticks was used as template. The results showed higher sensitivity for Borrelia detection as compared to standard dark-field microscopy. Method specificity was confirmed by cloning and sequencing of obtained 552 base pairs long amplicons. Phylogenetic analysis of obtained sequences showed that they belong to B. lusitaniae and B. afzelii genospecies. RT-PCR based method presented in this paper could be very useful as a screening test for detecting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia; RT-PCR; 16S rRNA; Ixodes ricinus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400015

Assessment of the risk of contracting Lyme disease in areas with significant human presence
Provedor de dados: 45
Autores: Milutinovic,M.; Radulovic,Z.; Tomanovic,S.
In order to describe seasonal changes in Lyme diseases risk rate at three localities in Serbia, during the period of 2003-2005, a total of 1542 Ixodes ricinus ticks (493 nymphs, 525 females and 524 males) were examined. The prevalence of Borrelia burgdorferi in Ixodes ricinus ticks at the Bovan Lake County were higher than the average for European localities (45.9% for adults and 18.8% for nymphs). In Mt. Avala and Kljajicevo counties adults and nymphs were, respectively, infected at the following percentages: 26.3, 10.7; 16.2 and 7.6%. The outcome indicates a relatively high risk of the contracting Lyme disease in all investigates areas.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tick; Ixodes ricinus; Lyme disease; Risk rate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100018

Molecular characterization of Borrelia burgdorferi sensu lato strains isolated in the area of Belgrade, Serbia
Provedor de dados: 58
Autores: Ristanovic,Elizabeta S.; Kitamura,Kayoko; Masuzawa,Toshiyuki; Milutinovic,Marija J.; Cekanac,Radovan M.; Stajkovic,Novica T.; Zivanovic,Danijela M.
This is the first report of the molecular characterization and identification of Borrelia burgdorferi sensu lato strains isolated in Serbia. Isolates A1, A2 and M1, from Ixodes ricinus, belong to Borrelia burgdorferi sensu stricto, while isolate K1 from Apodemus flavicollis is a mixture of Borrelia afzelii and B. burgdorferi s.s.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Borrelia burgdorferi s.l.; Ixodes ricinus; Lyme borreliosis; Serbia.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100004

PCR detection of Babesia microti and Babesia divergens in ticks
Provedor de dados: 19
Autores: Skotarczak, Bogumila; ACichocka, Gnieszka.
We investigated the presence of Babesia microti and B. divergens in Ixodes ricinus ticks collected in a Lyme borreliosis endemic area of Northern Poland. We used as the PCR target the fragment from a gene encoding the nuclear small-subunit ribosomal RNA (SS-rDNA). We have made a thorough analysis of the quantitative and rate per cent occurrence Ixodes ricinus from two areas of forest in province of Szczecin, known as highly recreative and frequented of many people resort. A total of 533 specimens collected in 13.3% was detected DNA of B. microti and in 3.0% of B. divergens.
Palavras-chave: Babesia microti; Babesia divergens; Ixodes ricinus; PCR.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/135