Consulta: subjectFacets:"Leishmania braziliensis"
Registros recuperados: 6
Data/hora: 01/06/2024 02:14:41

Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4)
Provedor de dados: 80
Autores: JG,Pinto; CP,Soares; J,Mittmann.
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Antiparasitic agents; Leishmania major; Leishmania braziliensis; AlPcS4; Photodynamic therapy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300010

American cutaneous leishmaniasis in dogs from an endemic urban area in Cianorte municipality, Paraná State, Brazil
Provedor de dados: 80
Autores: Cerino,DA; Veloso,J; Perles,TF; Zanzarini,PD; Lonardoni,MVC; Silveira,TGV.
American cutaneous leishmaniasis (ACL) was investigated in dogs from an urban endemic area in Cianorte, Paraná state, Brazil. Of 169 studied dogs, none presented suspected ACL lesions. Eleven animals (6.6%) had anti-Leishmania braziliensis antibodies (titers &gt; 40) detected by the immunofluorescent antibody test (IFAT) while four (2.4%) showed L. braziliensis-complex DNA by the polymerase chain reaction (PCR). Although no associations were found between IFAT or PCR results and age, sex, origin, free-roaming animals or length of residence at the address, the majority of IFAT- or PCR-positive dogs were from the urban area of the city and were allowed to roam freely beyond their neighborhood. The presence of anti-Leishmania braziliensis antibodies and L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania braziliensis; Fluorescent antibody technique; Polymerase chain reaction; Urban park.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200007

Intradermorreação de Montenegro em cães (Mammalia: Canidae) experimentalmente inoculados por Leishmaniaguyanensis e Leishmania braziliensis (Kinetoplastida: Trypanosomatidae), principais agentes causadores de Leishmaniose Tegumentar na Amazônia
Provedor de dados: 36
Autores: Reis,Sônia Rolim; Naiff,Rogério Farias; Almeida-Campos,Flávia Naief; Franco,Antonia Ramos.
O teste de intradermorreação de Montenegro é utilizado para detectar infecção por Leishmania em humanos. A técnica se baseia numa reação de hipersensibilidade tardia. Os antígenos de Montenegro utilizados no experimento são soluções de antígenos homólogos brutos de Leishmania (Viannia) braziliensis e L.(V)guyanensis. Este experimento demonstrou que os animais inoculados com as espécies de Leishmania inoculadas desenvolveram enduração no local do teste mais acentuada que os animais controle. Os resultados sugerem que o teste cutâneo pode vir a ser indicado como método auxiliar de diagnóstico em cães infectados por Leishmania sp.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intradermorreação de Montenegro; Leishmaniaguyanensis; Leishmania braziliensis; Cão; Amazônia.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000300027

Ocorrência de leishmaniose tegumentar em cães de área endêmica no Estado do Paraná
Provedor de dados: 45
Autores: Pittner,E.; Voltarelli,E.; Perles,T.F.; Arraes,S.M.A.A.; Silveira,T.G.V.; Lonardoni,M.V.C.
Investigou-se a infecção por parasitas do complexo Leishmania braziliensis em 50 cães da cidade de Maringá, PR, onde ocorreram casos humanos de leishmaniose tegumentar americana (LTA), entre agosto e dezembro de 2006. Os casos humanos de LTA ocorreram nos anos de 2003 a 2004. Nenhum animal apresentou lesão, mas 12 (24,0%) tinham sorologia e/ou PCR positivas, e a positividade tanto da IFI como da PCR foi de 14,0%. Trinta e cinco animais eram de residências, e oito deles (22,8%) tiveram testes laboratoriais para LTA positivos. Os outros 15 eram cães errantes, sendo que quatro deles (26,7%) apresentaram PCR positiva. A presença de animais com infecção assintomática por Leishmania em área onde a LTA é endêmica pode ser um indicador do potencial de sua...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cão; Homem; Leishmania braziliensis; Leishmaniose tegumentar americana.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300006

Diagnosis of American cutaneous leishmaniasis by enzyme immunoassay in patients from Northern Paraná State, Brazil
Provedor de dados: 55
Autores: Yoneyama,Kelly Aparecida Geraldo; Peder,Leyde Daiane de; Lonardoni,Maria Valdrinez Campana; Silveira,Thaís Gomes Verzignassi.
American cutaneous leishmaniasis (ACL) is an endemic disease caused by Leishmania parasites. The ACL diagnosis is commonly accomplished by parasitological and immunological methods. The objective of this work was to evaluate the enzyme immunoassay (EIA) for ACL laboratorial diagnosis. IgG antibodies against Leishmania (Viannia) braziliensis promastigotes were researched. For the method standardization 240 sera were used: 72 from patients with positive parasitological diagnosis, 38 from normal individuals and 113 from individuals with other pathologies. The sensibility was 93% and the specificity was 70%. Regarding diagnosis, EIA showed 97% positivity in patients with ACL and 97% negativity in patients with other cutaneous lesions. The EIA presented a good...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cutaneous leishmaniasis; Diagnosis; Antibodies; Immunoenzyme; Leishmania braziliensis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300012

Interleukin-10-dependent down-regulation of interferon-gamma response to Leishmania by Mycobacterium leprae antigens during the clinical course of a coinfection
Provedor de dados: 56
Autores: Azeredo-Coutinho,R.B.G.; Matos,D.C.S.; Nery,J.A.C.; Valete-Rosalino,C.M.; Mendonça,S.C.F.
We have described a case of a patient with an intriguing association of mucocutaneous leishmaniasis with lepromatous leprosy, two opposite polar forms of these spectral diseases. In the present follow-up study, we investigated the effect of the addition of Mycobacterium leprae antigens on interferon-gamma (IFN-γ) production in Leishmania antigen-stimulated cultures of peripheral blood mononuclear cells (PBMC) from this patient. For this purpose, PBMC cultures were stimulated with crude L. braziliensis and/or M. leprae whole-cell antigen extracts or with concanavalin A. In some experiments, neutralizing anti-human interleukin (IL)-10 antibodies were added to the cultures. IFN-γ and IL-10 levels in culture supernatants were measured by ELISA. During active...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Interferon-gamma; Interleukin-10; Mucocutaneous leishmaniasis; Lepromatous leprosy; Leishmania braziliensis; Mycobacterium leprae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700010