Consulta: subjectFacets:"Leishmania infantum"
Registros recuperados: 16
Data/hora: 16/06/2024 01:02:41

Cytological and molecular detection of Leishmania spp. in different biological tissues of dogs in areas endemic for visceral leishmaniasis
Provedor de dados: 45
Autores: Dias,A.F.L.R.; Almeida,A.B.P.F.; Rodrigues,J.Y.; Nakazato,L.; Fujimori,M.; Sousa,V.R.F.
RESUMO Devido à ampla distribuição da leishmaniose visceral (LV) no Brasil e à importância dos cães no ciclo de transmissão dessa zoonose, o presente estudo teve como objetivo avaliar a ocorrência de Leishmania spp. e caracterizar a espécie circulante em diferentes tecidos biológicos de cães da Baixada Cuiabana, Mato Grosso, Brasil. Amostras de sangue, linfonodo e medula óssea foram coletadas de 205 cães para realização de análise parasitológica por citologia e análise molecular por meio da nested PCR (nPCR) e do sequenciamento . Dos 205 cães estudados, 34 (16,58%) animais foram positivos pela nPCR, dos quais 12 possuíam formas amastigotas de Leishmania spp. na citologia. Amostras positivas na nPCR foram sequenciadas e caracterizadas como Leishmania...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visceral canine leishmaniosis; Leishmania infantum; PCR; Parasitology; Kala-azar.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000602103

Lesões orais secundárias à leishmaniose visceral em um cão com hiperadrenocorticismo endógeno: relato de caso
Provedor de dados: 45
Autores: Bochnakian,M.S.; Bezerra,J.A.B.; Fernandes,K.S.B.R.; Barros,V.R.; Paula,V.V.; Santos,J.P.S.; Filgueira,K.D.
RESUMO O objetivo do presente trabalho foi relatar um caso de leishmaniose visceral com apresentação mucosa em um cão com hiperadrenocorticismo. Um canino, macho, da raça Poodle, 11 anos de idade, foi atendido com histórico de disfagia, halitose e sialorreia. Ao exame físico, observou-se linfadenomegalia generalizada e alterações cutâneas, como rarefação pilosa, comedões, telangiectasia e atrofia cutânea. Além disso, o animal também apresentava formações orais localizadas na língua. Dos exames hematológicos e bioquímicos realizados, a única alteração encontrada foi elevação da fosfatase alcalina (1724u/L). O teste de supressão com a dexametasona em dose baixa foi executado para investigar hiperadrenocorticismo, tendo resultado positivo. Também foram...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Leishmaniose oral; Imunossupressão.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401115

Detection of Leishmania infantum in the smegma of infected dogs
Provedor de dados: 45
Autores: Silva,L.C.; Assis,V.P.; Ribeiro,V.M.; Tafuri,W.L.; Toledo Júnior,J.C.; Silva,S.O.; Melo,M.N.; Rachid,M.A.; Valle,G.R.
Considering the venereal transmission of visceral leishmaniasis from dogs to bitches, the aim of this study was to verify if the penile surface and smegma from infected dogs can be the source of parasites in bitches. Twelve Leishmania infantum infected dogs had semen and smegma samples collected for submission to PCR identification of the DNA of the parasite. Semen (41.7%) and smegma (50.0%) have similar positive incidence (P&gt;0.05; Fisher's exact test), with 58.3% of the dogs positive for semen and/or smegma samples. The proportion of positivity for both semen and smegma was 33.3%, but 8.3% was positive only for semen, and 16.7% only for smegma, revealing a moderate agreement between tests (K=0.5; Kappa index). It was concluded that Leishmania infantum...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Dog; Prepuce; Smegma; Semen.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300731

Avaliação da confiabilidade entre dois observadores em exames citopatológico e imunocitoquímico de aspirado de medula óssea no diagnóstico da leishmaniose visceral canina
Provedor de dados: 45
Autores: Vasconcelos,T.C.B.; Machado,L.C.; Abrantes,T.R.; Menezes,R.C.; Madeira,M.F.; Ferreira,L.C.; Figueiredo,F.B.
ABSTRACT Visceral leishmaniasis is a zoonosis in which the dog appears as the main source of infection in urban areas. Its diagnosis is complex and the cytopathological exam is a fast and cheap alternative to parasite direct visualization and its sensitivity can be increased by immunocytochemistry, though with a higher cost. The accuracy of such methods is dependent on the microscopist's experience and therefore, this study evaluated the reliability of such techniques between two observers, from bone marrow aspirates of 50 dogs from an endemic area for the disease. The parasitological culture in Novy-MacNeal-Nicolle medium was used as the reference standard. Among the main findings, the sensitivities obtained by observers I and II were respectively 62.5%...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cão; Leishmania infantum; Diagnóstico; Confiabilidade.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300821

Canine visceral leishmaniasis: a remarkable histopathological picture of one asymptomatic animal reported from Belo Horizonte, Minas Gerais, Brazil
Provedor de dados: 45
Autores: Xavier,S.C.; Chiarelli,I.M.; Lima,W.G.; Gonçalves,R.; Tafuri,W.L.
A remarkable histopathological picture of one asymptomatic dog naturally infected with Leishmania infantum (syn. chagasi) has been presented. Intracellular parasites were ease found in macrophages of all exanimated organs, especially in skin. Embedded paraffin tissues of liver, spleen, axillary and popliteal lymph nodes, and skin (ear, muzzle and abdomen) were stained by hematoxylin and eosin and by immunocytochemical reaction (streptoavidin-peroxidase method) to detect parasites. All organs showed an intense parasitism associated to severe pathological changes. All lymph nodes had conspicuous histological architecture alterations. Lymphocytes were replaced by macrophages stuffed with an intense number of amastigotes forms of Leishmania. The lymphoid...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dog; Leishmania infantum; Histopathology.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600004

Eco-epidemiological study on sandflies and environmental aspects related to the transmission of leishmaniasis in a municipality of Minas Gerais, Brazil, 2015-2016
Provedor de dados: 45
Autores: Gomes,L.B.; Dias,E.S.; Silva,S.C.P.F.; Carvalho,P.C.F.B.; Santos,A.G.R.C.; .Michalsky,E; Lara-Silva,F.O.; Souza,A.G.M.; Fortes-Dias,C.L.; Morais,M.H.F.; Nicolino,R.R.; Soares,D.F.M.
ABSTRACT The aim of this study was to evaluate Leishmania vectors and investigate the environmental and climatic characteristics related to their transmission. This was a prospective eco-epidemiological study involving the systematic collection of sandflies and a canine serological survey in areas of municipality in Brazil. Blood samples from 1,752 dogs were examined to test for canine visceral leishmaniasis (VL). An entomological evaluation was carried out using 24 light traps in the peridomicile and intradomicile areas of 12 households for 12 months. Climate variables were analyzed using a simple Spearman correlation and a spatial analysis via the fuzzy logic method and K function. The prevalence rate of canine VL was 4.1% and 7.1% in the two areas that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Visceral Leishmaniasis; Lutzomyia; Sandflies; Public health.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000601805

Ocular manifestations of leishmaniasis in a cat: first case report from Brazil
Provedor de dados: 45
Autores: Madruga,G.; Ribeiro,A.P.; Ruiz,T.; Sousa,V.R.F.; Campos,C.G.; Almeida,A.B.P.F.; Pescador,C.A.; Dutra,V.
ABSTRACT An 8-year-old domestic short hair female cat initially presented with bilateral uveitis with pseudotumoral appearance. The patient tested negative for feline immunodeficiency virus (FIV), feline leukemia virus (FeLV) and Toxoplasma gondii. Histopathology of a granulomatous lesion on the upper left conjunctiva revealed amastigotes compatible with Leishmania spp. Aqueous humor was aspired and the diagnosis was confirmed after isolation of promastigotes cultivated in biphasic NNN medium and by positive polymerase chain reaction (PCR) for Leishmania infantum. Treatment with allopurinol (10mg/kg/ BID/PO) was commenced and a natural insect repellent was prescribed. Six months of treatment with allopurinol associated with the initial topical medications...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cat; Uveitis; Leishmania infantum; Paracentesis.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501514

Citometria de fluxo no diagnóstico da leishmaniose visceral canina
Provedor de dados: 45
Autores: Carvalho Neta,A.V.; Rocha,R.D.R.; Gontijo,C.M.F.; Reis,A.B.; Martins-Filho,O.A.
Descreve-se a padronização de nova metodologia para detecção de anticorpos antiformas promastigotas fixadas de L. (L.) chagasi, por citometria de fluxo (AAPF-IgG), sua aplicabilidade e desempenho na identificação de casos de leishmaniose visceral canina (LVC). Foram avaliados dois grupos de cães classificados pela reação de imunofluorescência indireta (RIFI), como: não reatores (NR, n=10) e reatores (R, n=50) dos quais foram coletadas amostras de sangue (soro) para realização dos testes laboratoriais. Os resultados relacionados ao estabelecimento, aplicabilidade e desempenho da metodologia AAPF-IgG demonstraram que essa metodologia possibilita a identificação de uma região de reatividade diferencial entre cães NR e R, no soro diluído a 1:2048 e o valor de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniose visceral canina; Diagnóstico sorológico; Citometria de fluxo; Leishmania chagasi; Leishmania infantum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400005

Congenitally transmitted visceral leishmaniasis: report of two brazilian human cases
Provedor de dados: 55
Autores: Mescouto-Borges,Myrlena Regina Machado; Maués,Érika; Costa,Dorcas Lamounier; Pranchevicius,Maria Cristina da Silva; Romero,Gustavo Adolfo Sierra.
Visceral leishmaniasis is a relevant public health problem worldwide. Most of the reported cases in Latin America are from Brazil. Herein we report two human cases of congenitally transmitted visceral leishmaniasis in two patients who developed symptoms during pregnancy. The diagnosis was made by visual examination of Leishmania parasites in bone marrow aspirates of the mothers and by detecting parasite kDNA in bone marrow samples of the newborn children using polymerase chain reaction.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Congenital transmission; Visceral leishmaniasis; Leishmania infantum; Pregnancy.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200024

Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
Provedor de dados: 55
Autores: Khademvatan,Shahram; Gharavi,Mohammad Javad; Saki,Jasem.
OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Apoptosis; Gene expression.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500005

Epidemiological aspects of the first human autochthonous visceral leishmaniosis cases in Porto Alegre, Brazil
Provedor de dados: 55
Autores: Mahmud,Ibrahim Clós; Piassini,Letícia de Araujo Saraiva; Motta,Fabrizio; Behar,Paulo Renato Petersen; Souza,Getúlio Dornelles.
ABSTRACT Human visceral leishmaniasis is a growing anthropozoonosis in Brazil, and particularly in the southern region of the country. It is an infectious disease transmitted to humans, dogs and other animals in urban and rural areas of the Americas, mainly due to the bite of Lutzomya longipalpis infected with Leishmania infantum. This article aims to portray the current epidemiological situation of the human visceral leishmaniasis arrival in Porto Alegre city, located in the southern region of Brazil. It is a descriptive study, a case series and a critical review. Six human cases with human visceral leishmaniasis were notified by the date of conclusion of the study, all human visceral leishmaniasis cases were diagnosed at late stage, leading to four deaths.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Visceral leishmaniasis; Fever caused by sandflies; Communicable diseases; Leishmania infantum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000200124

Leishmania infantum heat shock protein 83 for the serodiagnosis of tegumentary leishmaniasis
Provedor de dados: 56
Autores: Celeste,B.J.; Angel,S.O.; Castro,L.G.M.; Gidlund,M.; Goto,H.
The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cross-reactivity has been pursued for the serodiagnosis of leishmaniasis. In the present study we analyzed the use of recombinant Leishmania infantum heat shock protein (Hsp) 83 in ELISA for the serodiagnosis of cutaneous (N = 12) and mucocutaneous leishmaniasis (N = 14) and we observed the presence of anti-L. infantum Hsp 83 antibodies in all samples as well as...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Leishmania infantum; Sp 83; ELISA; Serodiagnosis; Tegumentary leishmaniasis; Chagas' disease.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100001

Antileishmanial in vitro activity of essential oil from Myrciaria plinioides, a native species from Southern Brazil
Provedor de dados: 60
Autores: Kauffmann,Carla; Giacomin,Ana Caroline; Arossi,Kelen; Pacheco,Leandra Andressa; Hoehne,Lucélia; Freitas,Elisete Maria de; Machado,Gérzia Maria de Carvalho; Cavalheiro,Marilene Marcuzzo do Canto; Gnoatto,Simone Cristina Baggio; Ethur,Eduardo Miranda.
In South American folk medicine members of the genus Myrciaria are used for the treatment of malaria, diarrhoea, asthma, inflammation and post-partum uterine cleansing. The aim of this work was to evaluate its antileishmanial properties (in vitro) of essential oil derived from leaves of Myrciaria plinioides D. Legrand, a plant species that is native in South of Brazil. The essential oil was obtained by hydro-distillation using fresh leaves of M. plinioides. The chemical composition of this essential oil (MPEO, M. plinioides essential oil) was determined by gas chromatography coupled to mass spectrometry (GC-MS). MPEO was assayed in vitro for antileishmanial properties against promastigotes of Leishmania amazonensis and Leishmania infantum, and for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antileishmanial activity; Myrciaria plinioides; Myrtaceae; Leishmania amazonensis; Leishmania infantum.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100558

Synovial fluid as an auxiliary diagnostic tool for different stages of canine visceral leishmaniasis
Provedor de dados: 65
Autores: Rennó,Mário César; Vasconcellos,Amanda Otoni; Santos,Rogerio Rodrigues; Silva,Rodrigo Otávio Silveira; Carneiro,Rubens Antônio; Paes,Paulo Ricardo de Oliveira.
ABSTRACT:  Leishmaniasis represents a complex of chronic diseases with a broad geographic distribution and a high significance in public health worldwide. The varied clinical signs in conjunction with the low sensitivity and specificity of canine visceral leishmaniasis (CVL) detection methods make diagnosis of the disease complex. Among the several available laboratory tests, studies have suggested that the detection of parasites in synovial fluid (SF) is a good auxiliary tool in the diagnosis of CVL. However, no study has evaluated the influence of the clinical stage of CVL in the detection of Leishmania sp. in SF. This study aimed to evaluate the detection of Leishmania sp. amastigotes in the SF of dogs at different stages of the disease. The negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Zoonosis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500901

Leishmania infantum infection in dogs from maroon communities in the Eastern Amazon
Provedor de dados: 65
Autores: Silva,Andréia Ferreira da; Damasceno,Ágatha Rossanni Alves; Prado,Welton Seabra; Caldeira,Rossela Damasceno; Sampaio-Junior,Francisco Dantas; Farias,Diana Maria de; Silva,Laís Cristina Oliveira da; Guimarães,Ricardo José de Paula Souza e; Góes-Cavalcante,Gustavo; Scofield,Alessandra.
ABSTRACT: This study was designed to detect L. infantum infection in dogs and to evaluate the factors associated with canine visceral leishmaniasis in the maroon communities of Menino Jesus de Petimandeua and Itaboca in the municipality of Inhangapi, Pará, Brazil. Whole blood and intact skin samples were collected from 143 dogs, and a questionnaire was applied. L. infantum DNA was detected by polymerase chain reaction (PCR) using primers RV1 and RV2. Collection sites were georeferenced to obtain a spatial distribution of the residences visited and infected dogs. L. infantum DNA was detected in 8.4% (12/143) of the skin samples and in 1.4% (2/143) of the blood samples. On the risk map, three clusters were observed in Itaboca and one was observed in Menino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Maroon communities; Dog; PCR; Eastern Amazon.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100501

Prevalência e distribuição espacial da leishmaniose visceral em cães do município de Juatuba, Minas Gerais, Brasil
Provedor de dados: 65
Autores: Borges,Luiz Felipe Nunes Menezes; Lopes,Eliane Gonçalves Paiva; Freitas,Ana Cláudia Parreiras de; Silva,Marcos Xavier; Haddad,João Paulo Amaral; Silva,José Ailton da; Nicolino,Rafael Romero; Soares,Danielle Ferreira de Magalhães.
O presente estudo teve como objetivo determinar a prevalência e a distribuição espacial da leishmaniose visceral em cães (LVC) do município de Juatuba, Minas Gerais, Brasil, no ano de 2010. Foi realizado um estudo observacional transversal por meio de coleta de amostras sanguíneas de 957 cães, definidos aleatoriamente em todos os bairros do município. No diagnóstico sorológico, foram utilizados os testes de Imunofluorescência Indireta (IFI) e Teste de Adsorção Enzimática (ELISA), preconizados pelo Ministério da Saúde (MS). Foram marcadas as coordenadas geográficas dos imóveis participantes da pesquisa para verificar a distribuição espacial dos casos caninos. A prevalência da LVC foi estimada em 10,6%, com variação de 3 a 50%, distribuída em 70,6% dos...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania infantum; Georreferenciamento; Indicador epidemiológico.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000200025