Consulta: subjectFacets:"Lipid peroxidation"
Registros recuperados: 84
Data/hora: 14/06/2024 14:16:01

Активность ферментов пероксидного комплекса тканей мидии Mytilus galloprovincialis Lam в норме и в условиях естественного окислительного стресса
Provedor de dados: 13
Autores: Гостюхина, О. Л.; Солдатов, А. А.; Головина, И. В.
Исследовано содержание продуктов перекисного окисления липидов (ПОЛ) и состояние ферментативного пероксидного комплекса (ПК) (глутатионпероксидазы (ГП), каталазы и пероксидазы) в гепатопанкреасе, жабрах и ноге черноморского моллюска Mytilus galloprovincialis Lam. в условиях физиологического покоя и нереста. Последнее рассматривали как модель естественного окислительного стресса. Изучали особей двух цветовых морф - черной и коричневой. Выявлена тканевая специфика активностей ферментов и распределения продуктов ПОЛ в состоянии нормы, а также особенности ответных реакций тканей моллюсков обеих морф при действии окислительной нагрузки. Наиболее чувствительной к действию перекисных продуктов в условиях естественного окислительного стресса оказались особи черной...
Tipo: Journal Contribution
Palavras-chave: Ферменты; Каталаза; Пероксидаза; Глутатионпероксидаза; Перекисное окисление липидов; Окислительный стресс; Мидии; Цветовые морфы; Нерест; Enzymes; Catalase; Peroxidase; Glutathione peroxidase; Lipid peroxidation; Spawning; Oxidative stress; Mussels; Color morphs Spawning Enzymes Lipids Spawning Enzymes Lipids http://aims.fao.org/aos/agrovoc/c_14490 http://aims.fao.org/aos/agrovoc/c_2603 http://aims.fao.org/aos/agrovoc/c_4362.
Ano: 2005
URL: http://repository.ibss.org.ua/dspace/handle/99011/226

The influence of dietary supplementation of arachidonic acid on prostaglandin production and oxidative stress in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Seguineau, Catherine; Racotta, Ilie; Palacios, Elena; Delaporte, Maryse; Moal, Jeanne; Soudant, Philippe.
In a previous study, dietary supplementation with arachidonic acid (ARA) to oysters Crassostrea gigas increased haemocyte numbers, phagocytosis, and production of reactive oxygen species level (ROS) by haemocytes (Delaporte et al., 2006). To assess if the observed stimulation of these cellular responses resulted from changes of ARA-related prostaglandin (PG) production, we analysed prostaglandin E2 metabolite (PGEM) content on the same oysters fed three levels of ARA. Dietary supply of polyunsaturated fatty acids (PUFA) could also induce an oxidative stress that could similarly increase cellular responses; therefore, two indicators of oxidative stress were analysed: peroxidation level and antioxidant defence status. Together the observed positive...
Tipo: Text
Palavras-chave: Prostaglandins; N-6 unsaturated fatty acids; Lipid peroxidation; Antioxidants; ROS production.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00040/15103/12785.pdf

Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature
Provedor de dados: 5
Autores: Boutet, Isabelle; Jollivet, Didier; Shillito, Bruce; Moraga, Dario; Tanguy, Arnaud.
Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected...
Tipo: Text
Palavras-chave: Oyster crassostrea gigas; Heat shock protein; Mid atlantic ridge; Cytosolic malate dehydrogenases; Extracellular hemoglobins; Lipid peroxidation; Northeast pacific; Enzyme activities; Arginine kinase; Dual symbiosis.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/00129/24014/21970.pdf

Conjugated linoleic acid and fatty acid binding protein as antioxidants
Provedor de dados: 78
Autores: Piergiacomi,V.A.; Palacios,A.
Studies were carried out to determine the effect of conjugated linoleic acid (CLA) and rat liver cytosolic protein enriched in fatty acid binding protein (FABP) on the non-enzymatic lipid peroxidation of rat liver microsomes. The inhibition of lipid peroxidation was more evident when the FABP containing fraction obtained from CLA-group was used with either kind of microsomes (CLA and control). The chemiluminescence and polyunsaturated fatty acid composition of rat liver microsomes changed after CLA treatment. When native and peroxidized microsomes obtained from control group were compared, the most affected polyunsaturated fatty acids were: C18:2, C18:3 and C20:4, while in CLA-group C20:4 was mainly peroxidized The simultaneous analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conjugated Linoleic Acid; Fatty Acid Binding Protein; Lipid peroxidation; Liver; Microsomes.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982006000100013

Antioxidant activity of bee products added to water in tebuconazole-exposed fish
Provedor de dados: 83
Autores: Ferreira,Daiane; Unfer,Taís Cristina; Rocha,Hélio Carlos; Kreutz,Luiz Carlos; Koakoski,Gessi; Barcellos,Leonardo José Gil.
An experiment was conducted to evaluate the potential of honey, propolis, and bee pollen for the reversal of lipid peroxidation induced by tebuconazole (TEB) in South American catfish (Rhamdia quelen), in which the concentration of thiobarbituric acid reactive substances (TBARS), the activity of the antioxidant enzyme glutathione-S-transferase (GST) and the concentrations of non-enzymatic antioxidants, reduced glutathione (GSH), ascorbic acid, and non-protein thiols were assessed. Honey (0.125 g L-1) and bee pollen (0.05 g L-1) added to the water reverse the production of TBARS induced by TEB, while propolis demonstrated a pro-oxidant effect, inducing an increase in TBARS production. The data presented herein suggest that the addition of water to honey and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Bee pollen; Honey; Lipid peroxidation; Propolis; Tebuconazole.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000100021

Oxidative stress parameters in juvenile Brazilian flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae) exposed to cold and heat shocks
Provedor de dados: 83
Autores: Garcia,Luciano de O.; Okamoto,Marcelo H.; Riffel,Ana Paula K.; Saccol,Etiane M.; Pavanato,Maria A.; Sampaio,Luís André N.
The aim of this study was to determine oxidative stress parameters in the liver and gill of Brazilian flounder juveniles (307.0 ± 16.0 g and 30.0 ± 4.0 cm) submitted to different water temperature (17.1, 23.0 and 28.8ºC) for 72 h and maintained at salinity 25‰. After the acclimation of 7 days, in 23ºC, fish were transferred to 200 L tanks containing seawater (salinity 25‰) at 28.8ºC (heat shock), 17.1ºC (cold shock) or 23.0ºC (control), five replicates (five fish tank-1). The sampled collection occurred in 0 (pre-challenge), 3, 24, 48 and 72 h after temperature shock. Flounder exposed to 17.1ºC and 28.8ºC showed significantly higher TBARS levels and GST activity in the liver post-exposition (PE) in relation to the control (23ºC). CAT activity in liver...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; GST; Lipid peroxidation; TBARS; Thermal shocks.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300607

Biochemical and Physiological Changes in Rice Plants Due to the Application of Herbicides1
Provedor de dados: 87
Autores: LANGARO,A.C.; AGOSTINETTO,D.; OLIVEIRA,C.; SILVA,J.D.G.; BRUNO,M.S.
ABSTRACT The application of herbicides, even if selective, can cause biochemical and physiological changes, resulting in oxidative stress. This stress comes from the accumulation of reactive oxygen species produced due to exposure to the herbicide. However, plants have developed defense strategies, which can be enzymatic or non-enzymatic. The objective of this study was to evaluate the morphological and metabolic changes such as photosynthetic parameters, oxidative damage and antioxidant enzyme activity of rice plants after applying herbicides. For this, a study was conducted in a greenhouse and laboratory and the treatments consisted of application of imazapic + imazapyr, quinclorac, bentazon, cyhalofop-butyl, penoxsulan, bispyribac-sodium and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Oxidative stress; Lipid peroxidation; Selectivity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000200277

Lipid Peroxidation in the Plasma, Lungs, Heart and Liver of Broilers Fed a Grape Seed Extract and Raised at 2278 m of Altitude
Provedor de dados: 89
Autores: Rodríguez-Ortega,LT; Juárez-Juárez,G; Pro-Martínez,A; Sosa-Montes,E; Bautista-Ortega,J; González-Cerón,F; Vargas-Galicia,AJ; Chan-Díaz,D; Moreno-Medina,D; Gallegos-Sánchez,J; Rodríguez-Ortega,A.
ABSTRACT The aim of this research was to evaluate the effect of partial substitution of vitamin E (VE) by grape seed extract (GSE) on glutathione peroxidase activity (GPxA), nitric oxide (NO) concentration and lipid peroxidation [malondialdehyde (MDA) concentration] in the plasma, lungs, heart and liver, and live performance of broilers raised at 2278 m of altitude. One-d-old Ross 308 male chickens (n = 420) were randomly distributed into three treatments: Control-AL (basal diet containing 40 IU of VE and fed ad libitum; AL), Control-FR (basal diet and feed restriction; FR) and GSE-AL [basal diet containing 10 mg of GSE (equivalent to 30 IU VE) plus 10 IU of VE and fed AL]. The data were analyzed using one-way analysis of variance. Glutathione peroxidase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape seed extract; High altitude; Lipid peroxidation; Vitamin E.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300465

Supplementation of Natural Antioxidants to Reduced Crude Protein Diets for Japanese Quails Exposed to Heat Stress
Provedor de dados: 89
Autores: Şenay,S; Islim,P; Tugay,A.
ABSTRACT The study aimed at comparing the effects of the supplementation of natural antioxidant sources to a reduced crude protein diet on the performance, carcass traits, blood parameters, liver antioxidant enzyme activities and meat lipid peroxidation of Japanese quails exposed to heat stress. A total of 640 14-day-old male Japanese quails were exposed to two different temperature treatments (TT): thermoneutral (TN) or heat stress (HS)and were fed five different dietary treatments (DTs). A normal-protein diet (SCP) was formulated according to the National Research Council (24% CP). The reduced crude protein diet (RCP) was formulated to contain 3% less protein than that of the SCP diet. Three additional diets were prepared by supplementing the RCP with200...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Heat stress; Lipid peroxidation; Natural antioxidants; Quails; Performance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000100303

Biochemical Parameters and Histopathological Findings in the Forced Molt Laying Hens
Provedor de dados: 89
Autores: Mert,N; Yildirim,BA.
ABSTRACT The aim of this study was to evaluate the effects of forced molting using biochemical parameters and histopathological findings in laying hens. 36 Hyline W36 strain laying hens, 90 weeks old were chosen for this research. Eight of these chickens were randomly selected and placed in a cage as the control group before the molting program began. All the others 28 chickens were used for the forced molting program. Eight laying hens were slaughtered at the end of the molting program named as molting group. The remaining 20 hens were fed for 37 days, weighted and slaughtered when they reached the maximum egg production (80%) as postmolting group. Then, blood was analyzed for malondialdehyde, glutathione, catalase, glucose, calcium, phosphorus, albumin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant; Lipid peroxidation; Molting; Ovarium; Thyroid.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400711

SENSITIVITY TO ENVIRONMENTAL STRESS OF PRATA,JAPIRA AND VITÓRIA BANANA CULTIVARS PROVEN BY CHLOROPHYLL a FLUORESCENCE
Provedor de dados: 94
Autores: SANTOS,PRISCILA NOBRES DOS; SILVA,DIOLINA MOURA; GALLON,CAMILLA ZANOTTI; VENTURA,JOSÉ AIRES.
ABSTRACT  This study aimed to evaluate the physiological responses to environmental stress during pre- and post-harvest of the following banana cultivars: Prata (AAB), Japira (AAAB) and Vitoria (AAAB). Analyses were carried out on young plants at vegetative stage (daughter-plant) and adult plants at reproductive stage (motherplant). The experimental design was completely randomized. In the in vivo pre-harvest analysis were used seven replications, in a factorial scheme (3x2x2), three cultivars and two stages (vegetative and reproductive) and two collection periods (March and June). For the analysis of post-harvest quality were used five replications in a factorial design (3x2x5), corresponding to three cultivars, two development stages and five periods of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Musa spp; Chlorophyll fluorescence; Pigments; Lipid peroxidation; Post-harvest quality.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000200203

Effects of dietary propolis and vitamin E on growth performance and antioxidant status in blood of juvenile Rainbow trout, Oncorhynchus mykiss (Teleostei: Salmoniformes) under different flow rates
Provedor de dados: 103
Autores: Kelestemur,Gülüzar Tuna; Seven,Pinar Tatli; Yilmaz,Seval.
The present study investigated the effects of propolis and vitamin E supplementation in diets of juvenil rainbow trout subjected to two different flow rates with or without flow stress (0.9 and 2.1 l/min, respectively) on final weigth (FW), condition factor (CF), feed conservation ratio (FCR), protein efficiency ratio (PER) and vitamin A, C and E concentrations and malondialdehyde (MDA) levels in serum as well as plasma superoxide dismutase (SOD) activity. The experimental groups were as follows: group C was fed a basal diet, group P10 was fed a basal diet supplemented with 10 g propolis/kg, group P30 was fed a basal diet supplemented with 30 g propolis/kg, group E60 was fed a basal diet supplemented with 60 mg vitamin E/kg. CF and PER were not different...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Rainbow trout; Superoxide dismutase; Vitamin E.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702012000200001

In vitro determination of the antioxidant capacity of extracts and phenolic compounds from Ugni molinae Turcz. leaves
Provedor de dados: 98
Autores: Avello Lorca,Marcia; Pastene Navarrete,Edgar; González Riquelme,Margarita; Bittner Berner,Magalis; Becerra Allende,José.
Introduction: Ugni molinae Turcz., (Myrtaceae) is a plant that is distributed in central-southern of Chile, including Juan Fernández Islands. The leaves have been used in folk medicine for diarrhea and oral infections due to their astringent and antiseptic properties. Objective: antioxidant activity of two extracts (ethyl acetate and methanol) from U. molinae leaves was assessed in several assays. Methods: HPLC (high performance liquid chromatography) for chemical analysis and in vitro antioxidant methods. Results: HPLC profiling of both extracts showed the presence of flavan-3-ols (catechin) and gallic acid, flavonoids and caffeic acid derivates as main constituents. These extracts showed significant activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH),...
Tipo: Journal article
Palavras-chave: Antioxidant capacity; Lipid peroxidation; Ugni molinae.
Ano: 2013
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962013000400011

การประเมินการรั่วไหลของสารอิเล็กโทรไลต์ และระดับมาลอนไดแอลดีไฮด์ของใบอ้อย 10 สายพันธุ์ ภายใต้สภาวะขาดน้ำ
Provedor de dados: 106
Autores: Nisachon Jangpromma; Prasit Jaisil; Patcharin Songsri; Prasan Swatsitang; Sakda Daduang; Sompong Thammasirirak.
Water-deficit stress is a major abiotic stress that causes severe limitations on the productivity of sugarcane. To overcome of this problem, drought tolerance sugarcane cultivars should be developed and selected for planting. Therefore, this study aims to evaluate the variation of electrolyte leakage (EL) and malondialdehyde (MDA) content of sugarcane leaves under water-deficit stress conditions. The greenhouse experiment was conducted using two water regimes as the first factor [(i) well-watered and (ii) stressed at 90 to 100 days after planting (DAP) and recovery 100 to 110 DAP] and 10 sugarcane cultivars (Uthong 6, Khon Kaen 80, Khon Kaen 3, K86-161, 03-4-425, KU60-1, B34-164, Phill 66-07, Uthong 2 and LF82-2122) as the second factor. A 2 × 10 factorial...
Tipo: Collection
Palavras-chave: Drought; Lipid peroxidation; Saccharum officinarum; อ้อย; สายพันธุ์; ใบ; ปฏิกิริยาออกซิเดชันของไขมัน; สภาวะ-ขาดน้ำ; สารอิเล็กโทรไลต์; มาลอนไดแอลดีไฮด์; การรั่วไหลของสาร; เยื่อหุ้มเซลล์.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5318

Natural gaps associated with oxidative stress in Willisornis poecilinotus (Aves: Thamnophilidae) in a tropical forest
Provedor de dados: 36
Autores: Gomes,Andreza de Lourdes Souza; Gonçalves,Andrey Felipe Gomes; Vieira,José Luiz Fernandes; Marceliano,Maria Luiza Videiro; Silva,José Maria Cardoso da.
Natural disturbances in tropical forests modify the availability and quality of resources and alter the patterns of bird distribution. These environmental changes increase the metabolic rate and disrupt the redox balance promoting oxidative stress. This study aimed to compare the abundance of Willisornis poecilinotus between gaps and the understory of a forest with undisturbed canopy at Caxiuanã National Forest. The abundance was correlated with vegetation heights. The oxidative stress and the stress promoting factors were determined in both sites of sampling. We captured 81 specimens of W. poecilinotus. The number of captures was high in gaps. The specimens sampled at gaps showed high levels of oxidative stress. The biomarkers of oxidative stress were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Environmental disturbances; Redox balance; Lipid peroxidation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000200006

IMPACT OF FOLIAR APPLICATION OF ASCORBIC ACID AND α-TOCOPHEROL ON ANTIOXIDANT ACTIVITY AND SOME BIOCHEMICAL ASPECTS OF FLAX CULTIVARS UNDER SALINITY STRESS
Provedor de dados: 37
Autores: EL-BASSIOUNY,Hala M.S; SADAK,Mervat Sh.
The interactive effects of saline water (2000, 4000 and 6000 mg/l) and foliar application of 400 mg/l of ascorbic acid (Asc) or α- tocopherol (α-Toco) on three flax cultivars (Sakha 3, Giza 8 and Ariane) were conducted during two successive seasons 2011/2012 and 2012/2013. The results showed that total soluble carbohydrates, free amino acids and proline contents were significantly increased with increasing salinity levels in the all three tested cultivars except free amino acid content of Giza 8 which showed insignificant decrease. While, nucleic acids (DNA and RNA) showed significant decreases compared with the corresponding control. Moreover, applications of vitamins (Asc or α-Toco) as foliar spraying increased all mentioned contents compared to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Flax; Lipid peroxidation; Protein profile; Salinity; Α-Toco.
Ano: 2015
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000200021

Metallothionein and lipid peroxidation as markers to assess health status of chronically oiled Magellanic penguins in Argentina
Provedor de dados: 40
Autores: Romero,María Belén; Polizzi,Paula; Chiodi,Leila; Robles,Alicia; Rodríguez Heredia,Sergio; Gerpe,Marcela.
Chronic oiling affects seabirds, with short (individual survival) and long-term (dynamics of population) effects. Magellanic penguin is the most affected species in Argentina. The aim was to evaluate the use of blood biochemical-molecular biomarkers (MT and TBARs) to assess health status of Magellanic penguin exposed to chronic environmental oiling, in addition with routine hematological analyses (differential WBC count and PCV). Oiled specimens presented significant higher RBC metallothionein and TBARs concentrations than those from shelter; while routine hematological parameters were within normal levels for penguins and did not show any difference between both groups. These results demonstrated the higher sensibility of both biomarkers, evidencing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic oiling; Metallothionein; Lipid peroxidation; Magellanic penguin.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432015000100002

Essential oil of Tagetes filifolia against the four beetle Tribolium castaneum and its relation to acetylcholinesterase activity and lipid peroxidation
Provedor de dados: 114
Autores: Olmedo,R; Herrera,J.M; Lucini,E.I; Zunino,M.P; Pizzolitto,R.P; Dambolena,J.S; Zygadlo,J.A.
The development of natural insecticides would help to decrease the negative impact of synthetic insecticides. Fumigant toxicity of essential oil of Tagetes filifolia Lag (Asterales: Asteraceae) and its major compounds were evaluated against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). The essential oils were analyzed by gas chromatography-mass spectrometry. The essential oil and (E)-anethole were the most toxic at 24 h against adult insects (CL50 = 2.4 y 2.6 µL/mL water, respectively). Changes in the products of lipid peroxidation were evidenced by an increase in malondialdehyde content. Acetylcholinesterase activity in vitro, of T. castaneum adults, was also reported. (E)-anethole and estragole showed similar acetylcholinesterase inhibition (54...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fumigant toxicity; Tagetes filifolia; Tribolium castaneum; Lipid peroxidation; Acetylcholinesterase activity.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2015000200004

Temporal dynamics of the response to Al stress in Eucalyptus grandis × Eucalyptus camaldulensis
Provedor de dados: 42
Autores: Alcântara,Berenice K. de; Pizzaia,Daniel; Piotto,Fernando A.; Borgo,Lucélia; Brondani,Gilvano E.; Azevedo,Ricardo A.
Lipid peroxidation and root elongation of Eucalyptus grandis × Eucalyptus camaldulensis were studied under stress conditions in response to aluminum (Al), a metal known to limit agricultural productivity in acidic soils primarily due to reduced root elongation. In Brazil, the Grancam 1277 hybrid (E. grandis × E. camaldulensis) has been planted in the "Cerrado", a region of the country with a wide occurrence of acidic soils. The present study demonstrated that the hybrid exhibited root growth reduction and increased levels of lipid peroxidation after 24h of treatment with 100 µM of Al, which was followed by a reduction in lipid peroxidation levels and the recovery of root elongation after 48h of Al exposure, suggesting a rapid response to the early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aluminum; Eucalypt; Lipid peroxidation; Oxidative stress; Root growth.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201063

Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate
Provedor de dados: 42
Autores: Almeida,Andréa M.; Bertoncini,Clélia R.A.; Borecký,Jiri; Souza-Pinto,Nadja C.; Vercesi,Aníbal E.
Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron; Lipid peroxidation; Mitochondria; MtDNA; Oxidative stress.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300010

Oxidative stress responses of juvenile tambaqui Colossoma macropomum after short-term anesthesia with benzocaine and MS-222
Provedor de dados: 42
Autores: STRINGHETTA,GIOVANNA R.; BARBAS,LUIS A.L.; MALTEZ,LUCAS C.; SAMPAIO,LUÍS A.; MONSERRAT,JOSÉ M.; GARCIA,LUCIANO O.
ABSTRACT The present study aimed to evaluate the effects of benzocaine and tricaine methanesulfonate on oxidative stress parameters of juvenile tambaqui tissues. Fish (n=80) were anesthetized with benzocaine (100 mg L-1) or tricaine (240 mg L-1) and two control groups were used (non-anesthetized fish and fish exposed to ethanol-only). After anesthetic induction 10 fish/anesthetic were euthanized after 3, 12 and 24 hours post-anesthesia and tissue samplings (gills, liver and brain) were performed. Samples were submitted to analyses of enzyme activity glutathione-S-transferase (GST), cellular lipid peroxidation (TBARS) and total antioxidant capacity (ACAP). ACAP increased in gills of benzocaine treatment after 12 hours. The liver showed a reduction in ACAP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anesthetics; Fish; GST; Lipid peroxidation; Total antioxidant capacity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502209

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human’s erythrocytes
Provedor de dados: 42
Autores: BIANCHINI,MATHEUS C.; GALVÃO,DENNYURA O.; TAMBORENA,TATIANA; ALVES,CLAUDIA O.; PUNTEL,ROBSON L.
ABSTRACT Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mentha pulegium; Lamiaceae; Hemolysis; Lipid peroxidation; NPSH oxidation; Erythrocytes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602901

Lychee (Litchi chinensis Sonn.) peel flour: effects on hepatoprotection and dyslipidemia induced by a hypercholesterolemic diet
Provedor de dados: 42
Autores: QUEIROZ,ESTELA R.; ABREU,CELESTE M.P. DE; ROCHA,DENISE A.; SOUSA,RAIMUNDO V. DE; FRÁGUAS,RODRIGO M.; BRAGA,MARIANA A.; CÉSAR,PEDRO H.S.
ABSTRACT Dyslipidemias are associated with the incidence of cardiovascular diseases, obesity, diabetes, hypertension and hepatic steatosis, being the cause of morbidity and mortality. This study investigated the effects of lychee peel flour (PF) on serum levels of total cholesterol (TC), low-density lipoprotein (LDL-c), triacylglycerols (TAG) and various parameters related to obesity, in rats fed a hypercholesterolemic diet. Therefore, 20 male rats were used. In the first 21 days, the animals were fed a hypercholesterolemic diet, except for control group. In the following 21 days, their diets were modified, and they received a standard diet (Control); hypercholesterolemic (Hyper); hypercholesterolemic + 5% PF (PF5) and hypercholesterolemic + 10% PF (PF10)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cholesterol; Lipid peroxidation; Steatosis; Triacylglycerol; Weight gain.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100267

In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephalaL.
Provedor de dados: 42
Autores: LAHNECHE,AMINA M.; BOUCHEHAM,RATIBA; OZEN,TEVFIK; ALTUN,MUHAMMED; BOUBEKRI,NASSIMA; DEMIRTAS,IBRAHIM; BICHA,SABRINA; BENTAMENE,ALI; BENAYACHE,FADILA; BENAYACHE,SAMIR; ZAMA,DJAMILA.
Abstract: This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCELLigence RTCA instrument and photolyzing 46966 plasmid, respectively. The results exhibited that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Centaurea sphaerocephala; Phenolics; Antioxidant; Lipid peroxidation; Antiproliferative; DNA-damaged inhibition.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500619

Use of aqueous extract of noni in extender for sheep semen freezing
Provedor de dados: 45
Autores: Nascimento,A.L.C.; Santos,A.D.F.; Azevedo,H.C.; Velarde,J.M.D.S.; Lima,C.A.; Pereira,M.A.; Pinheiro,G.O.; Gomes,L.C.
ABSTRACT The study aimed to evaluate the action of aqueous extract of noni in an extender for sheep semen freezing. Treatments differed in inclusion of aqueous extract of noni in the extender: T1 ˗ no addition; T2 ˗ 24µg/mL; T3 ˗ 72µg/mL; and T4 ˗ 120µg/mL. Ejaculates were collected, diluted in the four treatments, and frozen. After thawing, the semen was subjected to a thermoresistance test and evaluated for subjective motility, vigor, membrane integrity assessment by hypo-osmotic swelling test, live-dead assay, computer-assisted sperm analysis and the status of sperm capacitation and acrosome reaction. Data were subjected to ANOVA, and then to Student Newman Keuls’s test at 5% significance level. In the thermoresistance test after two hours of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Concentrations; Lipid peroxidation; Plasma membrane; Spermatozoon.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501547

Antioxidant capacity of vitamin C in mouse liver and kidney tissues
Provedor de dados: 47
Autores: Weyers,Alicia; Ugnia,Laura I.; García Ovando,Hugo; Gorla,Nora B.
In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice. The initial nmol of lipid hydroperoxides/g of tissue were 137 ± 11 in the kidney and 145 ± 15 in the liver, and the nmol of TBARs were 13 ± 0.7 and 12 ± 0.6, respectively. Pre-treatment with vitamin C reduced the levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Vitamin C; Ciprofloxacin; Mice.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100003

Zinc in the prevention of Fe2initiated lipid and protein oxidation
Provedor de dados: 48
Autores: ZAGO,M. PAOLA; VERSTRAETEN,SANDRA V.; OTEIZA,PATRICIA I.
In the present study we characterized the capacity of zinc to protect lipids and proteins from Fe2+-initiated oxidative damage. The effects of zinc on lipid oxidation were investigated in liposomes composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS) at a molar relationship of 60:40 (PC:PS, 60:40). Lipid oxidation was evaluated as the oxidation of cis-parinaric acid or as the formation of 2-thiobarbituric acid-reactive substances (TBARS). Zinc protected liposomes from Fe2+ (2.5-50 muM)-supported lipid oxidation. However, zinc (50 muM) did not prevent the oxidative inactivation of glutamine synthelase and glucose 6-phosphate dehydrogenase when rat brain superntants were oxidized in the presence of 5 muM Fe2+ and 0.5 mM H2O2 .We also...
Tipo: Journal article
Palavras-chave: Aluminum; Catechins; Free radicals; Iron; Lipid peroxidation; Zinc.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200014

Antioxidant isolated from Schisandra propinqua (Wall.) Baill
Provedor de dados: 48
Autores: HUANG,FENG; XU,LIJIA; GANGGANG,SHI.
Schisandra propinqua (Wall.) Baill.(Schisandraceae) is widely used as a Chinese folk medicine. In this study, activity-guided fractionation of the ethanol extract from the stem of Schisandra propinqua led to the isolation of four extracts. Subsequently, a neolignan 4,4-di(4-hydroxy-3-methoxyphenly)-2,3-dimethylbutanol was isolated from the EtOAc part of the stem of Schisandra propinqua, the free radical scavenging activities of which were researched in vitro. The present work demonstrated that extracts and pure compound possessed scavenging activities to DPPH, superoxide anions and hydroxy radical, and could depress lipid peroxidation reaction induced by oxygen radical produced by the Fe2+/cysteine system in vitro. This suggests that the traditional...
Tipo: Journal article
Palavras-chave: Antioxidant; Free radical; Lipid peroxidation; Schisandra propinqua.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300009

Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats
Provedor de dados: 48
Autores: PRADEEP,KANNAMPALLI; RAJ MOHAN,CHANDRASEKARAN VICTOR; GOBIANAND,KUPANNAN; KARTHIKEYAN,SIVANESAN.
Diethylnitrosamine (DEN), found in many commonly consumed foods, is widely reported to induce cancer in animals and humans. The aim of the present study was to investigate the hepatoprotective and antioxidant activities of the leaf extract of the medicinal plant Cassia fistula Linn. against diethylnitrosamine induced liver injury in ethanol pretreated rats. Albino Wistar rats, pretreated with ethanol for 15 days, were administered a single dose of DEN. Thirty days after DEN administration, hepatotocellular damage was observed histologically, along with elevated levels of serum AST, ALT, ALP, LDH, &amp;#947;-GT and bilirubin and a simultaneous fall in the levels of the marker enzymes in the liver tissue. Liver oxidative stress was confirmed by elevated...
Tipo: Journal article
Palavras-chave: Antioxidants; Cassia fistula; Diethylnitrosamine; Hepatoprotective; Lipid peroxidation; Silymarin.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100013

Lipid peroxidation and antioxidant status in blood of patients with uterine myoma, endometrial polypus, hyperplastic and malignant endometrium
Provedor de dados: 48
Autores: PEJIC,SNEZANA; KASAPOVIC,JELENA; TODOROVIC,ANA; STOJILJKOVIC,VESNA; PAJOVIC,SNEZANA B.
Oxidative stress is considered to be involved in pathogenesis of many disorders of the female genital tract. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of uterine diseases in order to evaluate the extent of oxidative stress in blood of such patients. Blood samples of healthy subjects and gynecological patients were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides. The results show that alterations of measured parameters vary with the enzyme type and diagnosis. However, both reduction in antioxidants and elevation of lipid peroxidation were observed in general. Lipid...
Tipo: Journal article
Palavras-chave: Antioxidant enzymes; Endometrial polypus; Endometrial hyperplasia; Endometrial adenocarcinoma; Lipid peroxidation; Uterine myoma.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500005

Molecular and pathological basis of aceruloplasminemia
Provedor de dados: 48
Autores: KONO,SATOSHI; MIYAJIMA,HIROAKI.
Aceruloplasminemia is an autosomal recessive neurodegenerative disease characterized by iron accumulation in the brain as well as visceral organs. It is a loss-of-function disorder caused by mutations in the ceruloplasmin gene. Clinically, this disease consists of the triad of adult-onset neurological disease, retinal degeneration and diabetes mellitus. Massive iron accumulation and extensive loss of neurons are observed in the basal ganglia. The elevated iron concentration is associated with increased lipid peroxidation in the brains of aceruloplasminemia patients. Enlarged or deformed astrocytes and spheroid-like globular structures are characteristic neuropathological findings in aceruloplasminemia. Moreover, deformed astrocytes and globular structures...
Tipo: Journal article
Palavras-chave: Ceruloplasmin; Iron; Lipid peroxidation; Mutation.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100003

Increasing the rate of drying reduces metabolic imbalance, lipid peroxidation and critical water content in radicles of garden pea (Pisum sativum L.)
Provedor de dados: 48
Autores: Ntuli,Tobias M; Pammenter,Norman W; Berjak,Patricia.
Orthodox seeds become desiccation-sensitive as they undergo germination. As a result, germinating seeds serve as a model to study desiccation sensitivity in plant tissues. The effects of the rate of drying on the viability, respiratory metabolism and free radical processes were thus studied during dehydration and wet storage of radicles of Pisum sativum. For both drying regimes desiccation could be described by exponential and inverse modified functions. Viability, as assessed by germination capacity and tetrazolium staining, remained at 100% during rapid (&lt; 24 h) desiccation. However, it declined sharply at c. 0.26 g g¹ dm following slow (c. 5 days) drying. Increasing the rate of dehydration thus lowered the critical water content for survival. Rapid...
Tipo: Journal article
Palavras-chave: Antioxidant; Desiccation; Drying rate; Free radical processes; Lipid peroxidation; Metabolism; Respiration; Pisum sativum; Seed storage.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200002

Relationship between severity of adult community-acquired pneumonia and impairment of the antioxidant defense system
Provedor de dados: 48
Autores: Castillo,Rodrigo L; Carrasco,Rodrigo A; Álvarez,Pedro I; Ruiz,Mauricio; Luchsinger,Vivian; Zunino,Enna; Martínez,María A; Avendaño,Luis F.
Oxidant/antioxidant imbalance has been reported in some infectious diseases, including community-acquired pneumonia (CAP). The aim was to assess the antioxidant status in adults with CAP and its relationship with clinical severity at admission. Fifty-nine patients with CAP were enrolled and categorized at admission by the FINE score, from July 2010 to October 2012. In the same period 61 controls were enrolled. Plasma samples were obtained at admission for determination of the ferric reducing ability of plasma (FRAP) and lipid peroxidation (8-isoprostane). Erythrocyte reduced (GSH)/oxidized (GSSG) glutathione, malondialdehyde (MDA) and antioxidant enzyme activity were assessed. Antioxidant status in adults with CAP represented by FRAP and the GSH/GSSG ratio...
Tipo: Journal article
Palavras-chave: Pneumonia; Oxidative stress; Reduced glutathione; Lipid peroxidation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200013

Selected spices and their combination modulate hypercholesterolemia-induced oxidative stress in experimental rats
Provedor de dados: 48
Autores: Otunola,Gloria A; Oloyede,Oyelola B; Oladiji,Adenike T; Afolayan,Anthony J.
BACKGROUND: Effect of aqueous extracts of Allium sativum (garlic), Zingiber officinale (ginger), Capsicum fructensces (cayenne pepper) and their mixture on oxidative stress in rats fed high Cholesterol/high fat diet was investigated. Rats were randomly distributed into six groups (n&amp;#8201;=&amp;#8201;6) and given different dietary/spice treatments. Group 1 standard rat chow (control), group 2, hypercholesterolemic diet plus water, and groups 3, 4, 5, 6, hypercholesterolemic diet with 0.5 ml 200 mg&amp;#8201;·&amp;#8201;kg-1 aqueous extracts of garlic, ginger, cayenne pepper or their mixture respectively daily for 4 weeks. RESULTS: Pronounced oxidative stress in the hypercholesterolemic rats evidenced by significant (p&amp;#8201;&lt;&amp;#8201;0.05)...
Tipo: Journal article
Palavras-chave: Lipid peroxidation; Oxidative stress; Spices; Hypercholesterolemia; ROS.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100005

Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats
Provedor de dados: 48
Autores: Farías,Jorge G; Zepeda,Andrea B; Calaf,Gloria M.
Melatonin (N-acetyl-5-methoxytryptamine) is the main secretory product of the pineal gland in all mammals including humans, but it is also produced in other organs. It has been previously demonstrated to be a powerful organ-protective substance under oxidative stress conditions. The aim of this study was to evaluate the protective effect of melatonin in several organs such as heart, lung, kidney, and of the reproductive system, such as testis and epididymis in animals exposed to intermittent hypobaric hypoxia and therefore exposed to oxidative stress and analyzed by lipid peroxidation. Ten-week-old male Wistar rats were divided into 6 groups for 96 hours during 32 days under: 1) Normobaric conditions, 2) plus physiologic solution, 3) plus melatonin, 4)...
Tipo: Journal article
Palavras-chave: Intermittent hypobaric hypoxia; Lipid peroxidation; Melatonin; Oxidative stress.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100011

Antioxidant activity of extract from Polygonum aviculare L.
Provedor de dados: 48
Autores: HSU,CHIN-YUAN.
Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae) has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging...
Tipo: Journal article
Palavras-chave: Antioxidant activity; Free radical; Phenolics; Lipid peroxidation; DNA damage Polygonum aviculare L.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200010

Antioxidant activity of extract from Polygonum cuspidatum
Provedor de dados: 48
Autores: HSU,CHIN-YUAN; CHAN,YU-PEI; CHANG,JELI.
Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zuce has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value oí Polygonum cuspidatum extract is 110 µg/ml in free radical scavenging assays, 3.2 µg/ml in superoxide radical scavenging...
Tipo: Journal article
Palavras-chave: Antioxidant activity; Free radicals; Phenolics; Lipid peroxidation; DNA damage Polygonum cuspidatum.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100002

Positive effects of Green Tea on hepatic dysfunction, lipid peroxidation and antioxidant defence depletion induced by cadmium
Provedor de dados: 48
Autores: HAMDEN,KHALED; CARREA U,SERGE; AYADI MARKI,FATMA; MASMOUDI,HATEM; EL FEKI,ABDELFATTAH.
Cadmium (Cd) is a highly toxic environmental and industrial cumulative pollutant that affeets many organs, especially the liver. The present study was designed to evalúate the antioxidant effect of green tea on cadmium-induced hepatic dysfunction and oxidative stress in rats. Adult male Wistar rats were administered cadmium by injection of 20 emoles /kg bw/ every 3 days for six months. This study revealed significant (p &lt; 0.05) liver dysfunction, lipid peroxidation and a decline in antioxidant enzyme activities in the liver of cadmium-treated rats compared to control animáis. Compared to control rats, the activities of lactate dehydrogenase (LDH), gammaglutamyl transferase (GGT), acid phosphatase (PAC), phosphatase alkaline (PAL), as well as bilirubin...
Tipo: Journal article
Palavras-chave: Cadmium; Rat liver dysfunction; Green tea; Antioxidant enzymes; Lipid peroxidation.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300009

Oxidative stress and differential antioxidant enzyme activity in glyphosate-resistant and -sensitive hairy fleabane in response to glyphosate treatment
Provedor de dados: 53
Autores: Piasecki,Cristiano; Carvalho,Ivan Ricardo; Cechin,Joanei; Goulart,Francisco A. P; Maia,Luciano Carlos da; Agostinetto,Dirceu; Caverzan,Andréia; Stewart Jr.,C. Neal; Vargas,Leandro.
ABSTRACT Biochemical assays in a time-course were employed to evaluate stresses induced by glyphosate treatment in resistant and sensitive biotypes of Conyza bonariensis. Two experiments were conducted assessing glyphosate doses and time-course after treatment. The doses of glyphosate ranged from 0 to 11840 g ae·ha–1 and assessments performed until 552 h after glyphosate treatment (HAT). The objectives of this study were to evaluate the oxidative stress and differential antioxidant enzyme activity in glyphosate-resistant and -sensitive biotypes of hairy fleabane after glyphosate treatment. After treatment, both studied biotypes accumulated similar levels of shikimic-acid until 96 h. The sensitive biotype died at192 HAT. Shikimic-acid and hydrogen peroxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conyza bonariensis; Lipid peroxidation; Shikimic-acid; Herbicide resistance.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000300379

Drought stress mitigation with humic acid in two Cucumis melo L. genotypes differ in their drought tolerance
Provedor de dados: 53
Autores: Kıran,Servinç; Furtana,Gökçen Baysal; Talhouni,Manar; Ellialtıoğlu,Şeküre Şebnem.
ABSTRACT Different responses of two melon (Cucumis melo L.) genotypes (Şemame, drought and salt-tolerant and Ananas, drought and salt-sensitive) to drought stress with or without humic acid (HA) treatment were studied. The experiment was carried out under greenhouse conditions. The experimental design was two factorial randomized block with 4 replicates. HA treatment increased the shoot fresh and dry weights and leaf area of both genotypes under drought stress. HA stimulated accumulation of K and Ca ions, chlorophyll (SPAD value) and antioxidant enzyme activity (superoxide dismutase-SOD, catalase-CAT and glutathione reductase-GR) in both genotypes. This effect was more clear in the Şemame genotype than in Ananas.As a result, HA treatment has been proved to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melon; Drought; Plant breeding; Lipid peroxidation; Antioxidant enzymes.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000400490

Oxidative Damage and Antioxidant Response in Chenopodium murale L. Exposed to Elevated Levels of Zn
Provedor de dados: 52
Autores: Zoufan,Parzhak; Karimiafshar,Amer; Shokati,Sara; Hassibi,Payman; Rastegarzadeh,Saadat.
ABSTRACT Present study was carried out to investigate the effects of different Zn concentrations on growth and some antioxidant responses in Chenopodium murale L. The seeds were sown on the pots. 45-day plants were transferred to hydroponic solution containing 0.2 (control), 150, 300 and 600 µM Zn. The plants were harvested at 6 days after applying Zn treatments and some parameters were evaluated including plant length, fresh weight, photosynthetic pigments, malondialdehyde (MDA), ascorbate, proline and enzymatic antioxidants such as catalase (CAT), guaiacol peroxidase (GPX) and superoxide dismutase (SOD). Zn concentration showed a considerable increase in the shoot and root as the concentration of Zn increased in the medium, meanwhile the roots were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ascorbate; Enzymatic antioxidant; Lipid peroxidation; Proline; Zinc.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100418

Relationship between seminal malondialdehyde levels and sperm quality in fertile and infertile men
Provedor de dados: 52
Autores: Colagar,Abasalt Hosseinzadeh; Pouramir,Mehdi; Marzony,Eisa Tahmasbpour; Jorsaraei,Sayed Gholam Ali.
The aim of this study was to determine the level of malondialdehyde in seminal plasma of fertile and infertile men and investigate its relationship with sperm quality. Results showed that the mean of ± S.D. MDA concentration in seminal plasma of infertile men (0.94 ± 0.28 nmol/ml) was significantly higher than fertile men (0.65 ± 0.17 nmol/ml) (p value&lt; 0.001), and had negative relationship with sperm count, motility and morphology. Therefore it could be concluded that increase in lipid peroxidation was associated with sperm membrane destructed and high level of MDA.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seminal plasma; Male infertility; Sperm quality; Malondialdehyde (MDA); Lipid peroxidation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600010

Influence of chromium salts on increased lipid peroxidation and differential pattern in antioxidant metabolism in Pistia stratiotes L
Provedor de dados: 52
Autores: Upadhyay,RishiKesh; Panda,Sanjib Kumar.
In this work, the changing effect of different concentrations (0, 0.01, 0.1, 1, 10mM) of hexavalent and trivalent chromium on different biochemical parameters along with antioxidant enzymes was investigated on water lettuce (Pistia stratiotes L.) in order to know the possible involvement of this metal in oxidative injury, besides the activities of antioxidant enzymes leading to biochemical and oxidative aberration induced by elevated concentrations. Both in roots and shoots, Cr produced a significant increase in enzymic and non-enzymic antioxidants, except in catalase (CAT) activity where a strong accumulation of hydrogen peroxide was indicated, suggesting an imposition of oxidative stress. The observation showed an uptake of chromium by P. stratiotes L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biochemical alterations; Chromium stress; Lipid peroxidation; Oxidative stress; Pistia stratiotes L; Reactive oxygen species.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500018

Spirulina platensis Enhances the Beneficial Effect of Exercise on Oxidative Stress and the Lipid Profile in Rats
Provedor de dados: 52
Autores: Mazzola,Daiane; Fornari,Fernando; Vigano,Gabriela; Oro,Tatiana; Costa,Jorge Alberto Vieira; Bertolin,Telma Elita.
ABSTRACT This study aimed to evaluate the effect of Spirulina platensis and moderate exercise on oxidative stress and lipid profiles in the rats. Forty male Wistar rats were allocated to the following 10-week treatments, three times a week: exercise (E, 30 min swimming),S. platensis (SP, 26 mg/Kg); exercise andSpirulina (ES); and control (C). Outcomes were Thiobarbituric Acid Reactive Substances (TBARS) in serum and brain, and cholesterol and triglycerides (TG) in serum. Rats treated with exercise showed lower brain TBARS than the controls, mostly in association withS. platensis. In the groups E and ES, serum TBARS decreased after intervention. Compared with the controls, both E and ES prevented an increase in cholesterol and reduced triglycerides. Results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise; Antioxidant; Cholesterol; Lipid peroxidation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600961

Phenolic Content and Biomolecule Oxidation Protective Activity of Globularia alypum Extracts
Provedor de dados: 52
Autores: Bouriche,Hamama; Kada,Seoussen; Senator,Abderrahmane; demirtas,Ibrahim; Ozen,Tevfik; Toptanci,Bircan Çeken; Kizil,Goksel; Kizil,Murat.
ABSTRACT The protective activity of methanolic (Met E) and aqueous (Aq E) extracts of Globularia alypum L. (G. alypum) against DNA, lipid and protein oxidative damage was investigated. Moreover, the scavenging, chelating, and reducing power activities of the extracts were also evaluated. Phytochemical analysis was performed to determine phenolic compounds. Results showed that Met E and Aq E were rich in phenolic compounds, and were able to scavenge DPPH˙ with IC50 values of 48.61 µg/mL and 51.97 µg/mL, respectively. In addition, both extracts were able to chelate ferrous ions. At 300 μg/mL, the chelating activity was 97.53% and 91.02%, respectively. The reducing power of these extracts was also remarkable and concentration dependent. At 100 µg/mL, both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; DNA damage; Globularia alypum; Lipid peroxidation; Protein oxidation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100422

Peripheral markers of oxidative stress in chronic mercuric chloride intoxication
Provedor de dados: 56
Autores: Gutierrez,L.L.P.; Mazzotti,N.G.; Araújo,A.S.R.; Klipel,R.B.; Fernandes,T.R.G.; Llesuy,S.F.; Belló-Klein,A.
The present study was designed to evaluate the time course changes in peripheral markers of oxidative stress in a chronic HgCl2 intoxication model. Twenty male adult Wistar rats were treated subcutaneously daily for 30 days and divided into two groups of 10 animals each: Hg, which received HgCl2 (0.16 mg kg-1 day-1), and control, receiving the same volume of saline solution. Blood was collected at the first, second and fourth weeks of Hg administration to evaluate lipid peroxidation (LPO), total radical trapping antioxidant potential (TRAP), and superoxide dismutase (Cu,Zn-SOD), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and catalase (CAT). HgCl2 administration induced a rise (by 26%) in LPO compared to control (143 ± 10 cps/mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Heavy metals; Antioxidant enzymes; Lipid peroxidation; Total radical-trapping antioxidant potential; HgCl2.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600009

Intensity of swimming exercise influences aortic reactivity in rats
Provedor de dados: 56
Autores: Brito,A.F.; Silva,A.S.; Souza,I.L.L.; Pereira,J.C.; Silva,B.A. da.
Exercise is known to cause a vasodilatory response; however, the correlation between the vasorelaxant response and different training intensities has not been investigated. Therefore, this study evaluated the vascular reactivity and lipid peroxidation after different intensities of swimming exercise in rats. Male Wistar rats (aged 8 weeks; 250-300 g) underwent forced swimming for 1 h whilst tied to loads of 3, 4, 5, 6, and 8% of their body weight, respectively (groups G3, G4, G5, G6 and G8, respectively; n=5 each). Immediately after the test, the aorta was removed and suspended in an organ bath. Cumulative relaxation in response to acetylcholine (10−12-10−4 M) and contraction in response to phenylephrine (10−12-10−5 M) were measured. Oxidative stress was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aerobic exercise; Anaerobic exercise; Lipid peroxidation; Smooth muscle; Swimming; Vessel.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100996

Aluminum induces lipid peroxidation and aggregation of human blood platelets
Provedor de dados: 56
Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL) of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Aluminum; Lipid peroxidation; Chemiluminescence; Platelet aggregation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500005

Antioxidant and free radical scavenging effects in extracts of the medicinal herb Achyrocline satureioides (Lam.) DC. ("marcela")
Provedor de dados: 56
Autores: Desmarchelier,C.; Coussio,J.; Ciccia,G.
Achyrocline satureioides (Lam.) DC. (Compositae) is a medicinal herb used in Argentina, Uruguay, Brazil and Paraguay for its choleretic, antispasmodic and hepatoprotective properties. The presence of the flavonoid quercetin and its derivatives, and of different phenolic acids such as caffeic, chlorogenic and isochlorogenic acids in the aerial parts of this plant has led us to study the antioxidant activity of its extracts using different bioassays. The inhibition of luminol-enhanced chemiluminescence by the aqueous and methanolic extracts was used to show that their total reactive antioxidant potential index (TRAP; in µM Trolox equivalents) was 91.0 ± 15.4 and 128.1 ± 20.1 µM, respectively, while the total antioxidant reactivity index (TAR) was calculated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Achyrocline satureioides; Antioxidant activity; Luminol-enhanced chemiluminescence; Thiobarbituric acid-reactive substances; Hydroperoxide-initiated chemiluminescence; Lipid peroxidation; DNA damage; Reactive oxygen species.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900010

Ambient particulate air pollution from vehicles promotes lipid peroxidation and inflammatory responses in rat lung
Provedor de dados: 56
Autores: Pereira,C.E.L.; Heck,T.G.; Saldiva,P.H.N.; Rhoden,C.R.
Oxidative stress plays a major role in the pathogenesis of particle-dependent lung injury. Ambient particle levels from vehicles have not been previously shown to cause oxidative stress to the lungs. The present study was conducted to a) determine whether short-term exposure to ambient levels of particulate air pollution from vehicles elicits inflammatory responses and lipid peroxidation in rat lungs, and b) determine if intermittent short-term exposures (every 4 days) induce some degree of tolerance. Three-month-old male Wistar rats were exposed to ambient particulate matter (PM) from vehicles (N = 30) for 6 or 20 continuous hours, or for intermittent (5 h) periods during 20 h for 4 consecutive days or to filtered air (PM &amp;lt;10 µm; N = 30). Rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Reactive oxygen species; Lipid peroxidation; Particulate air pollution; Lung inflammation; Acute effects.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000008

Swimming training attenuates oxidative damage and increases enzymatic but not non-enzymatic antioxidant defenses in the rat brain
Provedor de dados: 56
Autores: Nonato,L.F.; Rocha-Vieira,E.; Tossige-Gomes,R.; Soares,A.A.; Soares,B.A.; Freitas,D.A.; Oliveira,M.X.; Mendonça,V.A.; Lacerda,A.C.; Massensini,A.R.; Leite,H.R.
Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise therapy; Oxidative stress; Rat; Lipid peroxidation; Superoxide dismutase; Swimming training.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000605

Protection against cephalosporin-induced lipid peroxidation and nephrotoxicity by (+)-cyanidanol-3 and vitamin E
Provedor de dados: 56
Autores: Cojocel,C.; Tolle,K.-L.; El-Hajj,H.; Baumann,K.
The ability of the clinically used cephalosporins: cephalothin, cefotaxime and cefotiam to induce lipid peroxidation (LPO) and renal damage was compared to that of nephrotoxic cephaloridine under in vivo conditions. Glutathione was measured in rat liver or in renal cortex as non-protein sulfhydryls. LPO was measured in plasma, renal cortex and liver by the generation of malondialdehyde or as the increase in renal cortical concentration of conjugated dienes. Impairment of renal function was measured as the decrease in renal cortical accumulation of the organic anion p-aminohippurate (PAH). Administration of cephalosporins to rats as a single dose (2000 mg/kg, ip) induced a significant glutathione-depletion in the renal cortex with cephaloridine, and in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cephalosporins; Glutathione depletion; Lipid peroxidation; Nephrotoxicity; Antioxidants; Vitamin E.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600017

Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
Provedor de dados: 56
Autores: Almeida,M.B.; Costa-Malaquias,A.; Nascimento,J.L.M.; Oliveira,K.R.; Herculano,A.M.; Crespo-López,M.E.
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphine; Glia; Oxidative stress; Hydrogen peroxide; Lipid peroxidation; Opiod.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000500398

Association between dental-oral health in young adults and salivary glutathione, lipid peroxidation and sialic acid levels and carbonic anhydrase activity
Provedor de dados: 56
Autores: Öztürk,L.K.; Furuncuoğlu,H.; Atala,M.H.; Uluköylü,O.; Akyüz,S.; Yarat,A.
The aim of the present study was to evaluate the relationship between salivary oxidative stress and dental-oral health. Healthy young adults, matched for gender and age, with (N = 21, 10 men, mean age: 20.3 ± 1 years) and without (N = 16, 8 men, mean age: 21.2 ± 1.8 years) caries were included in this study. The World Health Organization (WHO) caries diagnostic criteria were used for determining the decayed, missing, filled teeth (DMFT) index. The oral hygiene and gingival status were assessed using the simplified oral hygiene index and gingival index, respectively. Unstimulated salivary total protein, glutathione (GSH), lipid peroxidation and total sialic acid levels, carbonic anhydrase activity, and salivary buffering capacity were determined by standard...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Glutathione; Lipid peroxidation; Sialic acid; Carbonic anhydrase; Dental-oral health.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100003

Effect of silybin on high-fat-induced fatty liver in rats
Provedor de dados: 56
Autores: Yao,Jiayin; Zhi,Min; Minhu,Chen.
Silybin, a natural antioxidant, has been traditionally used against a variety of liver ailments. To investigate its effect and the underlying mechanisms of action on non-alcoholic fatty liver in rats, we used 60 4-6-week-old male Sprague-Dawley rats to establish fatty liver models by feeding a high-fat diet for 6 weeks. Hepatic enzyme, serum lipid levels, oxidative production, mitochondrial membrane fluidity, homeostasis model assessment-insulin resistance index (HOMA-IR), gene and protein expression of adiponectin, and resistin were evaluated by biochemical, reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. Compared with the model group, silybin treatment (26.25 mg·kg-1·day-1, started at the beginning of the protocol)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Non-alcoholic fatty liver disease; Mitochondrial membrane fluidity; Lipid peroxidation; Insulin resistance; Silybin.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700007

Myocardial antioxidant and oxidative stress changes due to sex hormones
Provedor de dados: 56
Autores: Barp,J.; Araújo,A.S.R.; Fernandes,T.R.G.; Rigatto,K.V.; Llesuy,S.; Belló-Klein,A.; Singal,P.
The purpose of the present study was to examine myocardial antioxidant and oxidative stress changes in male and female rats in the presence of physiological sex hormone concentrations and after castration. Twenty-four 9-week-old Wistar rats were divided into four groups of 6 animals each: 1) sham-operated females, 2) castrated females, 3) sham-operated males, and 4) castrated males. When testosterone and estrogen levels were measured by radioimmunoassay, significant differences were observed between the castrated and control groups (both males and females), demonstrating the success of castration. Progesterone and catalase levels did not change in any group. Control male rats had higher levels of glutathione peroxidase (50%) and lower levels of superoxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catalase; Glutathione peroxidase; Lipid peroxidation; Superoxide dismutase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900008

Effects of rosiglitazone on serum paraoxonase activity and metabolic parameters in patients with type 2 diabetes mellitus
Provedor de dados: 56
Autores: Atamer,Y.; Atamer,A.; Can,A.S.; Hekimoğlu,A.; Ilhan,N.; Yenice,N.; Koçyiğit,Y.
Human serum paraoxonase contributes to the anti-atherogenic effect of high-density lipoprotein cholesterol (HDL-C) and has been shown to protect both low-density lipoprotein cholesterol (LDL-C) and HDL-C against lipid peroxidation. We investigated the effects of rosiglitazone on paraoxonase activity and metabolic parameters in patients with type 2 diabetes mellitus [50 patients (30 males, 20 females); mean±SD age: 58.7±9.2 years, body mass index: 28.2±4.1'kg/m2], in whom glucose control could not be achieved despite treatment with metformin, sulphonylurea, and/or insulin. The patients were given 4'mg/day rosiglitazone for 3 months in addition to their usual treatment. Serum paraoxonase activity, malondialdehyde, homocysteine, and lipid profile were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosiglitazone; Diabetes mellitus type 2; Paraoxonase; Lipoproteins; Homocysteine; Lipid peroxidation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600528

Elevated levels of erythrocyte-conjugated dienes indicate increased lipid peroxidation in schistosomiasis mansoni patients
Provedor de dados: 56
Autores: Facundo,H.T.F.; Brandt,C.T.; Owen,J.S.; Lima,V.L.M.
Schistosoma mansoni causes liver disease by inducing granulomatous inflammation. This favors formation of reactive oxygen species, including superoxide ions, hydrogen peroxide and hydroxyl radicals all of which may induce lipid peroxidation. We have evaluated lipid peroxidation in 18 patients with hepatosplenic schistosomiasis mansoni previously treated with oxamniquine followed by splenectomy, ligature of the left gastric vein and auto-implantation of spleen tissue, by measuring levels of erythrocyte-conjugated dienes and plasma malondialdehyde (MDA). Age-matched, healthy individuals (N = 18) formed the control group. Erythrocyte-conjugated dienes were extracted with dichloromethane/methanol and quantified by UV spectrophotometry, while plasma MDA was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Lipid peroxidation; Conjugated dienes; Malondialdehyde; Schistosomiasis mansoni.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700003

Incorporation of dietary trans monounsaturated fatty acids into tissues of Walker 256 tumor-bearing rats
Provedor de dados: 56
Autores: Moreira,N.X.; Curi,R.; Padovese,R.; Mancini-Filho,J.
The correlation between dietary trans fatty acids and neoplasia was examined in the present study. Walker 256 tumor-bearing and control rats were fed a trans monounsaturated fatty acid (MUFA)-rich diet for 8 weeks and the incorporation of trans fatty acids by tumor tissue was examined. Also, the effect of tumor growth on trans fatty acid composition of plasma and liver, and the content of thiobarbituric acid-reactive substances (TBARS) was determined. Walker 256 tumor cells presented both trans and cis MUFAs given in the diet. The equivalent diet proportions were 0.66 for trans and 1.14 for cis. Taking into consideration the proportion of trans MUFAs in plasma (11.47%), the tumor incorporated these fatty acids in a more efficient manner (18.27%) than the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trans fatty acids; Walker 256 tumor; Fatty acid composition; Lipid peroxidation.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400010

Long-term melatonin treatment reduces ovarian mass and enhances tissue antioxidant defenses during ovulation in the rat
Provedor de dados: 56
Autores: Chuffa,L.G.A.; Amorim,J.P.A.; Teixeira,G.R.; Mendes,L.O.; Fioruci,B.A.; Pinheiro,P.F.F.; Seiva,F.R.F.; Novelli,E.L.B.; Mello Júnior,W.; Martinez,M.; Martinez,F.E.
Melatonin regulates the reproductive cycle, energy metabolism and may also act as a potential antioxidant indoleamine. The present study was undertaken to investigate whether long-term melatonin treatment can induce reproductive alterations and if it can protect ovarian tissue against lipid peroxidation during ovulation. Twenty-four adult female Wistar rats, 60 days old (± 250-260 g), were randomly divided into two equal groups. The control group received 0.3 mL 0.9% NaCl + 0.04 mL 95% ethanol as vehicle, and the melatonin-treated group received vehicle + melatonin (100 µg·100 g body weight-1·day-1) both intraperitoneally daily for 60 days. All animals were killed by decapitation during the morning estrus at 4:00 am. Body weight gain and body mass index...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melatonin; Ovary mass; Lipid peroxidation; Antioxidant defenses; Superoxide dismutase.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300007

Ginseng administration protects skeletal muscle from oxidative stress induced by acute exercise in rats
Provedor de dados: 56
Autores: Voces,J.; Cabral de Oliveira,A.C.; Prieto,J.G.; Vila,L.; Perez,A.C.; Duarte,I.D.G.; Alvarez,A.I.
Enzymatic activity was analyzed in the soleus, gastrocnemius (red and white) and plantaris muscles of acutely exercised rats after long-term administration of Panax ginseng extract in order to evaluate the protective role of ginseng against skeletal muscle oxidation. Ginseng extract (3, 10, 100, or 500 mg/kg) was administered orally for three months to male Wistar rats weighing 200 ± 50 g before exercise and to non-exercised rats (N = 8/group). The results showed a membrane stabilizing capacity of the extract since mitochondrial function measured on the basis of citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities was reduced, on average, by 20% (P &lt; 0.05) after exercise but the activities remained unchanged in animals treated with a ginseng...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Panax ginseng; Exercise; Free radicals; Lipid peroxidation; Glutathione status; Mitochondria.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200012

Inhibition of lipid peroxidation and iron (II)-dependent DNA damage by extracts of Pothomorphe peltata (L.) Miq
Provedor de dados: 56
Leaves of Pothomorphe peltata (L.) Miq. (Piperaceae) are used locally as anti-inflammatory, antipyretic, hepatoprotective and diuretic infusions and to treat external ulcers and local infections in several parts of the Peruvian, Bolivian and Brazilian Amazon region. The antioxidant activity of different extracts of P. peltata was studied using the hydroperoxide-initiated chemiluminescence assay in liver homogenates, and the methanolic extract was found to have the highest antioxidant activity, with an IC50 = 4 µg/ml. Aqueous and dichloromethane extracts did not show antioxidant activity. The extracts were further evaluated using the thiobarbituric acid-reactive substances (TBARS) assay. Finally, an assay of DNA sugar damage induced by Fe (II) salt was used...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Pothomorphe peltata; Antioxidant activity; Hydroperoxide-initiated chemiluminescence; Thiobarbituric acid-reactive substances; DNA-binding activity; Lipid peroxidation.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100014

Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark
Provedor de dados: 56
Autores: Kumar,R.S.; Sivakumar,T.; Sunderam,R.S.; Gupta,M.; Mazumdar,U.K.; Gomathi,P.; Rajeshwar,Y.; Saravanan,S.; Kumar,M.S.; Murugesh,K.; Kumar,K.A.
The present study was carried out to evaluate the antioxidant and antimicrobial activities of a methanol extract of Bauhinia racemosa (MEBR) (Caesalpiniaceae) stem bark in various systems. 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, superoxide anion radical, nitric oxide radical, and hydroxyl radical scavenging assays were carried out to evaluate the antioxidant potential of the extract. The antioxidant activity of the methanol extract increased in a concentration-dependent manner. About 50, 100, 250, and 500 µg MEBR inhibited the peroxidation of a linoleic acid emulsion by 62.43, 67.21, 71.04, and 76.83%, respectively. Similarly, the effect of MEBR on reducing power increased in a concentration-dependent manner. In DPPH radical scavenging assays the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bauhinia racemosa; Antioxidant activity; Lipid peroxidation; Scavenging superoxide; DPPH assay; Antimicrobial activity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000700004

Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes
Provedor de dados: 56
Autores: Latha,M.; Pari,L.
The effects of an aqueous extract of the plant Scoparia dulcis (200 mg/kg) on the polyol pathway and lipid peroxidation were examined in the liver of streptozotocin adult diabetic male albino Wistar rats. The diabetic control rats (N = 6) presented a significant increase in blood glucose, sorbitol dehydrogenase, glycosylated hemoglobin and lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides, and a significant decrease in plasma insulin and antioxidant enzymes such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) compared to normal rats (N = 6). Scoparia dulcis plant extract (SPEt, 200 mg kg-1 day-1) and glibenclamide (600 µg kg-1 day-1), a reference drug, were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glutathione peroxidase; Glutathione-S-transferase; Insulin; Lipid peroxidation; Scoparia dulcis; Sorbitol dehydrogenase; Diabetes.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400015

Nitric oxide, cholesterol oxides and endothelium-dependent vasodilation in plasma of patients with essential hypertension
Provedor de dados: 56
Autores: Moriel,P.; Sevanian,A.; Ajzen,S.; Zanella,M.T.; Plavnik,F.L.; Rubbo,H.; Abdalla,D.S.P.
The objective of the present study was to identify disturbances of nitric oxide radical (·NO) metabolism and the formation of cholesterol oxidation products in human essential hypertension. The concentrations of·NO derivatives (nitrite, nitrate, S-nitrosothiols and nitrotyrosine), water and lipid-soluble antioxidants and cholesterol oxides were measured in plasma of 11 patients with mild essential hypertension (H: 57.8 ± 9.7 years; blood pressure, 148.3 ± 24.8/90.8 ± 10.2 mmHg) and in 11 healthy subjects (N: 48.4 ± 7.0 years; blood pressure, 119.4 ± 9.4/75.0 ± 8.0 mmHg).Nitrite, nitrate and S-nitrosothiols were measured by chemiluminescence and nitrotyrosine was determined by ELISA. Antioxidants were determined by reverse-phase HPLC and cholesterol oxides...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitrotyrosine; S-nitrosothiols; Nitrate; Oxidized LDL; Lipid peroxidation; Endothelin-1.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100007

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice
Provedor de dados: 56
Autores: Bhuvaneswari,V.; Velmurugan,B.; Abraham,S.K.; Nagini,S.
Chemoprotection by dietary agents is a promising strategy for cancer prevention. The aim of the present study was to evaluate the combined effect of tomato and garlic against 7,12-dimethylbenz- [a]anthracene (DMBA)-induced genetic damage and oxidative stress in 12-14-week-old male Swiss albino mice. The animals were randomized into experimental and control groups and divided into eight groups of five animals each. Group 1 animals were injected intraperitoneally with 35 mg/kg body weight DMBA suspended in peanut oil as a single dose. Groups 2-4 animals received tomato (500 mg/kg body weight), garlic (125 mg/kg body weight) and a combination of tomato and garlic for 5 days by gavage, respectively, followed by DMBA 1.5 h after the final feeding. The doses of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Antioxidants; DMBA; Garlic; Lipid peroxidation; Micronuclei; Tomato; Lycopene.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700012

Effect of bullfrog (Rana catesbeiana) oil administered by gavage on the fatty acid composition and oxidative stress of mouse liver
Provedor de dados: 56
Autores: Silva,L.P.; Miyasaka,C.K.; Martins,E.F.; Leite,J.R.S.A.; Lacava,Z.G.M.; Curi,R.; Azevedo,R.B.
The aim of the present study was to investigate the effects of daily intragastric administration of bullfrog oil (oleic, linoleic and palmitoleic acid-rich oil), corresponding to 0.4% of body weight for four weeks, on fatty acid composition and oxidative stress (lipid peroxidation and catalase activity) in mouse liver. The activities of aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and gamma-glutamyltransferase (GGT), biomarkers of tissue injury, were determined in liver homogenates and serum. The proportions of 18:2n-6, 20:4n-6, 20:5n-3, and 22:6n-3 (polyunsaturated fatty acids, from 37 to 60%) in the total fatty acid content were increased in the liver of the bullfrog oil-treated group (P &lt; 0.05)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bullfrog oil; Liver; TBARS; Catalase activity; Lipid peroxidation; Monounsaturated fatty acid.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000007

Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris
Provedor de dados: 58
Autores: Zepeda,Andrea B.; Figueroa,Carolina A.; Pessoa,Adalberto; Farías,Jorge G.
ABSTRACT The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris. We evaluated cell viability using propidium iodide by flow cytometry and thiobarbituric acid reactive substances to measure cell membrane damage. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Pyruvate pathway; Yeast.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400856

The impacts of Elaeagnus umbellata Thunb. leaf and fruit aqueous extracts on mice hepatic, extrahepatic antioxidant and drug metabolizing enzymes related structures
Provedor de dados: 60
Autores: Ozen,Tevfik; Yildirim,Kemal; Toka,Mehmet.
ABSTRACT In this work, the potential chemopreventive activities of Elaeagnus umbellata fruit aqueous (EUFA) and leaf aqueous (EULA) extracts focusing on the modulatory influence of xenobiotic metabolizing enzymes (XMEs), antioxidant enzymes, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), lactate dehydrogenase (LDH) activity, lipid peroxidation (LP), sulfhydryl groups were investigated in the hepatic and extrahepatic organs of Swiss albino mice (50 and 100 mg/kg body wt given orally for 14 days) and compared with BHA (0.75 % in diet). The modulatory and chemopreventive properties of two different doses EUFA and EULA were observed for cytochrome P450, cytochrome b5, sulfhydryl groups, NADPH-cytochrome P450 reductase,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elaeagnus umbellata Thunb; Chemopreventive properties; Xenobiotic metabolizing enzymes; Antioxidant enzymes; Lipid peroxidation; Glutathione; Lactate dehydrogenase; Glucose-6-phosphate dehydrogenase; 6-Phosphogluconate dehydrogenase.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000300625

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats
Provedor de dados: 60
Autores: Duarte,Valéria Morgiana Gualberto; Rodrigues,Alanna de Sousa; Rezende,Luciana Augusto de; Ramos,Ana Maria de Oliveira; Souza,Rodrigo Medeiros de; Freire Neto,Francisco Paulo; Medeiros,Aldo da Cunha; Brandão Neto,José; Almeida,Maria das Graças; Rezende,Adriana Augusto de.
The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship among lipid peroxidation index, antioxidant defense parameters and bone metabolism in a premenopausal diabetic model using measures including thiobarbituric acid-reactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes was induced by alloxan and confirmed by glycemia &amp;gt;250 mg/dL. The lipid peroxidation index, measured by TBARS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxidative stress; Lipid peroxidation; Diabetes mellitus; Diabetic osteopenia/experimental study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300018

Cadmium toxicity causes oxidative stress and induces response of the antioxidant system in cucumber seedlings
Provedor de dados: 61
Autores: Gonçalves,Jamile F.; Becker,Alexssandro G.; Cargnelutti,Denise; Tabaldi,Luciane A.; Pereira,Luciane B.; Battisti,Vanessa; Spanevello,Rosélia M.; Morsch,Vera M.; Nicoloso,Fernando T.; Schetinger,Maria R.C.
In this study, the effects of cadmium (Cd) on lipid peroxidation, electrolyte leakage, protein oxidation, ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid, non-protein thiol groups and total soluble protein contents in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing four Cd levels as CdCl2 (0, 100, 400, and 1000 µmol L-1) for 10 d. The lowest Cd level decreased the malondialdehyde concentration. Electrolyte leakage increased only at 1000 µmol Cd L-1, whereas protein oxidation and total soluble protein content were enhanced at 400 and 1000 µmol Cd L-1. Activity of APX was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Ascorbic acid; Cucumis sativus; Heavy metal; Lipid peroxidation; Protein oxidation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300006

Response of Cucumis sativus L. seedlings to Pb exposure
Provedor de dados: 61
Autores: Gonçalves,Jamile F.; Becker,Alexssandro G.; Pereira,Luciane B.; Rocha,João B. T. da; Cargnelutti,Denise; Tabaldi,Luciane A.; Battisti,Vanessa; Farias,Júlia G.; Fiorenza,Amanda M.; Flores,Érico M. M.; Nicoloso,Fernando T.; Schetinger,Maria R. C.
In this study, the effects of lead (Pb) on growth, photosynthetic pigments concentration, lipid peroxidation, electrolyte leakage percentage (ELP), protein oxidation, aminolevulinate dehydratase (ALA-D; E.C. 4.2.1.24), ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid (AsA), non-protein thiol groups (NPSH) and total soluble protein concentrations in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing three Pb levels as (C2H3O2)Pb.3H2O (0, 100, 400, and 1000 µmol L-1) for 10 d. Increasing Pb concentrations in substrate enhanced Pb concentration in both roots and shoot. Pb...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Cucumber; Lipid peroxidation; Pb; Photosynthetic pigments; Protein oxidation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300002

Glycinebetaine improves salt tolerance in vinal (Prosopis ruscifolia Griesbach) seedlings
Provedor de dados: 61
Autores: Meloni,Diego A.; Martínez,Carlos A.
Glycinebetaine (GB) is a very important organic osmolyte that accumulates in a number of diverse groups of plants in response to environmental stress. In some plants, increased resistance to drought, salinity and low temperature has been achieved through exogenous application of GB. In this study, the effect of exogenously applied GB (8 mM) on the ability of vinal (Prosopis ruscifolia G.) plants to withstand NaCl stress was investigated. The dry biomass of vinal showed a decrease under salt stress, but in GB-treated plants exposed to the same stress, this reduction was mitigated. Sodium accumulated in the leaves of plants grown under saline conditions, but the addition of GB to salt-grown plants reduced Na+ content by 40%. Salinity significantly reduced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Salt stress; Superoxide dismutase.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300007

Physiological and biochemical changes occurring in dwarf-cashew seedlings subjected to salt stress
Provedor de dados: 61
Autores: Abreu,Carlos E.B. de; Prisco,José T.; Nogueira,Ana R.C.; Bezerra,Marlos A.; Lacerda,Claudivan F. de; Gomes-Filho,Enéas.
The effects of salt stress on some physiological and biochemical traits were evaluated in dwarf-cashew seedlings at the same developmental stage. Seeds were sown in trays containing vermiculite moistened with distilled water or with NaCl solutions having different electrical conductivities: 0.7, 1.8, 6.0, 9.8, 13.4, 17.4 and 20.6 dS m-1. Salinity delayed and inhibited seedling growth and development, particularly in the shoot. Concentrations of Na+ and Cl-, but not of K+, increased with increasing stress severity. With the exception of proline, concentration of organic solutes was only marginally affected by salt stress. Catalase activity in leaves increased slightly as a result of salt stress, whereas guaiacol peroxidase activity was induced only under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anacardium occidentale; Antioxidant enzymes; Lipid peroxidation; Oxidative stress; Protein pattern; Salt stress.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000200003

Oxidative processes during 'Golden' papaya fruit ripening
Provedor de dados: 61
Autores: Resende,Evellyn Couto Oliveira; Martins,Paula Fabiane; Azevedo,Ricardo Antunes de; Jacomino,Angelo Pedro; Bron,Ilana Urbano.
'Golden' papayas at maturity stage 1 (15% yellow skin) were chosen to study selected oxidative processes, the activity of antioxidant enzymes and lipid peroxidation in storage at 22°C, during the ripening of the fruit. An increase in ethylene production was observed on the second day of storage and it was followed by an increase in respiration. An increased activity of catalase, glutathione reductase and ascorbate peroxidase was observed concurrently or soon after this increase in ethylene production and respiration. The increased activity of these enzymes near the peaks of ethylene production and respiration is related to the production of oxidants accompanying the onset of ripening. On the fourth day of storage, there was an increased lipid peroxidation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carica papaya; Antioxidant system; Climacteric; Ethylene; Lipid peroxidation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200002

Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in Chickpea
Provedor de dados: 61
Autores: Kumari,Anita; Sheokand,Sunita; Swaraj,Kumari.
The present study investigates the effect of long and short term Cd stress in chickpea plants and evaluates the protective effect of exogenous nitric oxide (NO) supplementation using sodium nitroprusside (SNP). Cadmium treatments were given before sowing (long term stress) and thirty days after germination (short term stress). Sodium nitroprusside was given as foliar spray 30 days after germination to both long and short term Cd treated plants. Cadmium adversely affected the membranes as was evident from increased electrolyte leakage and lipid peroxidation levels. Sodium nitroprusside treatments decreased ion leakage and lipid peroxidation levels significantly. Short term Cd stress resulted in a higher induction of the catalase, peroxidase, ascorbate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Lipid peroxidation; Membrane injury; Sodium nitroprusside.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000400007

Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants
Provedor de dados: 61
Autores: Sheokand,Sunita; Bhankar,Vinita; Sawhney,Veena.
Accumulation of salts in irrigated soils is one of the primary factors limiting yield in South Asia. We investigated whether exogenous nitric oxide (NO) supplementation as sodium nitroprusside has any ameliorating effect against NaCl induced oxidative damage in chickpea leaves. NaCl treatment (250 mM) alone and in combination with two concentrations of SNP (0.2 and 1 mM) were given to 50 days old chickpea plants for 2, 4 and 6 days. Salt stress adversely affected the relative membrane injury, lipid peroxidation levels, relative water content (RWC) and H2O2 content. The effect was time dependent. SNP treatments could ameliorate the toxic effect of short term salt stress of 2 days on relative membrane injury and partial amelioration was observed with 4 and 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Chickpea; Lipid peroxidation; Nitric oxide; Salt stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200002

Oxidative stress in five wheat varieties (Triticum aestivum L.) exposed to water stress and study of their antioxidant enzyme defense system, water stress responsive metabolites and H2O2 accumulation
Provedor de dados: 61
Autores: Chakraborty,Usha; Pradhan,Bhumika.
Five varieties of wheat (Triticum aestivum L.) - KW, UP 2752, PBW 343, SO and LV - were subjected to water stress and sampling was done on the 3rd, 6th and 9th day of stress. RWC decline in KW, UP 2752 and PBW 343 (36.65, 42.34 and 40.75% respectively) was comparatively lesser than in LV and SO (52.93 and 52.67% respectively). In all varieties tested, three antioxidant enzymes (POX, APOX and GR) showed an initial increase. The activity of POX and GR increased with the increase in the duration of stress in KW, UP 2752 and PBW 343, while the activity of APOX declined. However, CAT and SOD showed an initial increase in these varieties, whereas it declined in SO and LV with increase in the period of stress. Accumulation of H2O2 declined during prolonged water...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Carotenoids; Drought; Lipid peroxidation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000200005

The effect of chilling on the photosynthetic activity in coffee (Coffea arabica L.) seedlings: The protective action of chloroplastid pigments
Provedor de dados: 61
Autores: Oliveira,Jurandi Gonçalves de; Alves,Pedro Luis C.A.; Magalhães,Antonio Celso.
Coffea arabica is considered to be sensitive to low temperatures, being affected throughout its entire life cycle. Injury caused by chilling (low temperatures above zero degree centigrade) is characterized primarily by inhibition of the photosynthetic process. The objective of this work was to evaluate the role of photosynthetic pigments in the tolerance of coffee (C. arabica L.) seedlings to chilling. The evaluation the photosynthetic activity was made by emission of Chl a fluorescence at room temperature (25 ºC) in vivo and in situ, using a portable fluorometer. The pigment content was obtained by extraction with 80 % acetone, while estimation of membrane lipid peroxidation was determined by measuring the MDA content in leaf tissue extracts. The results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorophyll a fluorescence; Coffee; Lipid peroxidation; Photosynthetic pigments.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200003

Antioxidant-enzymatic system of two sorghum genotypes differing in salt tolerance
Provedor de dados: 61
Autores: Costa,Paulo Henrique Alves da; Neto,André Dias de Azevedo; Bezerra,Marlos Alves; Prisco,José Tarquinio; Gomes-Filho,Enéas.
Two forage sorghum genotypes were studied: CSF18 (salt-sensitive) and CSF20 (salt-tolerant). Shoot growth reduction as a result of salt stress was stronger in the salt sensitive genotype compared to the salt tolerant one. When the two genotypes were subjected to salt stress (75 mM NaCl) no significant change in lipid peroxidation was observed. However, salt stress induced increases in superoxide dismutase and catalase activities in both genotypes. These salt-induced increases were higher in the salt-tolerant genotype. Peroxidase activity was differentially affected by salt stress in the two genotypes. The activities of these peroxidases were decreased by salt stress in the salt-sensitive genotype and increased in the salt-tolerant genotype. In addition,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorghum bicolor; Antioxidant-enzymatic system; Lipid peroxidation; Oxidative stress; Salinity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000400003

Photo-oxidative stress in coconut seedlings: early events to leaf scorching and seedling death
Provedor de dados: 61
Autores: Naresh,Kumar S.; Kasturi Bai,K.V.
Experiments were conducted on coconut seedlings to delineate events of photo-oxidative stress damage. Studies on chlorophyll fluorescence indicated a clear case of excess light energy under high light conditions causing stress to coconut seedlings raised under coconut palms. Quantum yield of photo-chemistry of leaflets exposed to high light was significantly less than those under shade. Seedlings exposed to high light and then shifted to shade have shown significant improvement in quantum yield. Excess light energy harvested by chlorophyll antenna caused high non-photochemical quenching resulting in production of biologically toxic super oxide, hydrogen peroxide and hydroxyl radicals. It is apparent that photoinhibition of photosynthesis takes place due to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; Chlorophyll fluorescence; Oxidative stress; Photosynthesis; Lipid peroxidation; Gas exchange; Proteins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202009000300006

Can Triamcinolone acetonide, platelet-rich plasma, and pentosan polysulfate sodium induce oxidative stress in cultured equine chondrocytes?
Provedor de dados: 65
Autores: Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Wolkmer,Patrícia; Salazar,Rodrigo Fernando dos Santos; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Brass,Karin Erica; Corte,Flavio Desessards De La.
ABSTRACT:  Progressive deterioration and loss of articular cartilage are the final degenerative events common to osteoarthritis (OA). Reactive oxygen species (ROS) play an important role in this chondrocyte catabolic activity, leading to cell death and matrix components breakdown. Intra-articular corticosteroid injections such as triamcinolone acetonide have been used to control pain and inflammation associated with OA. New treatments for OA, platelet-rich plasma and pentosan polysulphate sodium have also been used and further investigations are necessary to determine their safety in joint cells. In this in vitro study, the use of these three substances (triamcinolone acetonide, platelet-rich plasma, and pentosan polysulphate sodium) in healthy...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Catalase; Glutathione peroxidase; Lipid peroxidation.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700602

ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
Provedor de dados: 65
Autores: Oliveira,Karen Maciel de; Binda,Nancy Scardua; Lavor,Mário Sérgio Lima; Silva,Carla Maria Osório; Rosado,Isabel Rodrigues; Taguchi,Tatiana Malagoli; Alves,Endrigo Gabellini Leonel; Melo,Marília Martins; Gomez,Marcus Vinícius; Melo,Eliane Gonçalves de.
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MVIIA; Cone snail; Cell viability; Glutamate; Reactive oxygen species; Lipid peroxidation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150

Differential responses between mature and young leaves of sunflower plants to oxidative stress caused by water deficit
Provedor de dados: 65
Autores: Cechin,Inês; Corniani,Natália; Fumis,Terezinha de Fátima; Cataneo,Ana Catarina.
The effects of water stress and rehydration on leaf gas exchange characteristics along with changes in lipid peroxidation and pirogalol peroxidase (PG-POD) were studied in mature and in young leaves of sunflower (Helianthus annuus L.), which were grown in a greenhouse. Water stress reduced photosynthesis (Pn), stomatal conductance (g s), and transpiration (E) in both young and mature leaves. However, the amplitude of the reduction was dependent on leaf age. The intercellular CO2 concentration (Ci) was increased in mature leaves but it was not altered in young leaves. Instantaneous water use efficiency (WUE) in mature stressed leaves was reduced when compared to control leaves while in young stressed leaves it was maintained to the same level as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photosynthesis; Antioxidant enzymes; Lipid peroxidation; Rehydration; Helianthus annuus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000600008