Consulta: subjectFacets:"Listeria monocytogenes"
Registros recuperados: 57
Data/hora: 07/06/2024 02:30:08

Inhibition mechanism of Listeria monocytogenes by a bioprotective bacteria Lactococcus piscium CNCM I-4031
Provedor de dados: 5
Autores: Saraoui, Taous; Fall, Papa-abdoulaye; Leroi, Francoise; Antignac, Jean-philippe; Chereau, Sylvain; Pilet, Marie France.
Listeria monocytogenes is a pathogenic Gram positive bacterium and the etiologic agent of listeriosis, a severe food-borne disease. Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp. To investigate the inhibititory mechanism, a chemically defined medium (MSMA) based on shrimp composition and reproducing the inhibition observed in shrimp was developed. In co-culture at 26°C, L. monocytogenes was reduced by 3-4 log CFU g-1 after 24 h. We have demonstrated that the inhibition was not due to secretion of extracellular antimicrobial compounds as bacteriocins, organic acids and hydrogen peroxide. Global metabolomic fingerprints of these strains in pure culture were assessed by...
Tipo: Text
Palavras-chave: Listeria monocytogenes; Lactococcus piscium; Nutrients competition; Cellular contact; Chemically defined medium; Bacterial interaction.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00250/36123/34677.pdf

High-Throughput Identification of Candidate Strains for Biopreservation by Using Bioluminescent Listeria monocytogenes
Provedor de dados: 5
Autores: El Kheir, Sara M.; Cherrat, Lamia; Awussi, Ahoefa A.; Ramia, Nancy E.; Taha, Samir; Rahman, Abdur; Passerini, Delphine; Leroi, Francoise; Petit, Jeremy; Mangavel, Cecile; Revol-junelles, Anne-marie; Borges, Frederic.
This article describes a method for high-throughput competition assays using a bioluminescent strain of L. monocytogenes. This method is based on the use of the luminescent indicator strain L. monocytogenes EGDelux. The luminescence of this strain is correlated to growth, which make it suitable to monitor the growth of L. monocytogenes in mixed cultures. To this aim, luminescence kinetics were converted into a single numerical value, called the Luminescence Disturbance Indicator (LDI), which takes into account growth inhibition phenomena resulting in latency increase, decrease in the luminescence rate, or reduction of the maximum luminescence. The LDI allows to automatically and simultaneously handle multiple competition assays which are required for...
Tipo: Text
Palavras-chave: Biopreservation; Listeria monocytogenes; High-throughput screening assays; Bioluminescence; Competition; Mixed culture; Bioprotection; Antibacterial activities.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00454/56534/58236.pdf

Applications of protective cultures, bacteriocins and bacteriophages in fresh seafood and seafood products
Provedor de dados: 5
Autores: Pilet, Marie-france; Leroi, Francoise.
Microbial seafood-borne disease represents 10 to 20% of the total food-borne outbreaks. Most of them are from bacterial origin and involve seafood products that have been contaminated by pathogenic Vibrio, Listeria monocytogenes and histaminogen bacteria. On the other hand, seafood products are very sensitive to the development of spoiling bacteria producing off-odours. Pathogenic and spoiling microflora are not always reduced or limited by the processing steps that are currently used in these foodstuffs, and the interest for alternative techniques such as bioprotection to improve quality and safety of seafood has increased in the last years. Among the microbial flora of lightly preserved seafood products, lactic acid bacteria usually become dominant...
Tipo: Text
Palavras-chave: Biopreservation of seafood; Seafood safety and spoilage; Lactic acid bacteria; Sensory quality; Listeria monocytogenes.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00030/14138/11384.pdf

Biodiversity of Listeria monocytogenes sensitivity to bacteriocin-producing Carnobacterium strains and application in sterile cold-smoked salmon
Provedor de dados: 5
Autores: Brillet, Anne; Pilet, M; Prevost, H; Bouttefroy, A; Leroi, Francoise.
The aim of this study was to demonstrate the inhibitory capacity of Carnobacterium strains against a collection of Listeria monocytogenes strains in cold-smoked salmon (CSS).  Methods and Results: Three bacteriocin-producing strains, Carnobacterium divergens V41, C. piscicola V1 and C. piscicola SF668, were screened for their antilisterial activity against a collection of 57 L. monocytogenes strains selected from the French smoked salmon industry, using an agar spot test. All the Listeria strains were inhibited but three different groups could be distinguished differing in sensitivity to the three Carnobacterium strains. However, C. divergens V41 always had the highest inhibitory effect. The antilisterial capacity was then tested in sterile CSS blocks...
Tipo: Text
Palavras-chave: Listeria monocytogenes; Cold smoked salmon; Carnobacterium; Biopreservation; Bacteriocin.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/publication-610.pdf

Adaptative response of Listeria monocylogenes to osmotic and chill stress: consequences on food safety.
Provedor de dados: 5
Autores: Elmnasser, Noura; Ritz Bricaud, Magali; Guillou, Sandrine; Leroi, Francoise; Orange, Nicole; Bakhrouf, Amira; Federighi, Michel.
Listeria monocytogenes is it psychrotrophic food-borne pathogen. This organism is problematic for food production industry due to its ubiquitous distribution ill nature and its tolerance to condition of osmotic and chill stress. One common strategy employed by many bacteria and by L. monocytogenes for adaptation to osmotic stress is by the intracellular accumulation of compatible solutes. Two different types of adaptative reponses to low temperature have undertaken by L. monocytogenes. One of the responses is accomplished by adjusting membrane fluidity through alteration of the membrane fatty acid composition. A second response consists ill the accumulation of osmolytes. In this brief review we will focus on these general systems Which are Of particular...
Tipo: Text
Palavras-chave: Food safety; Chill stres; Osmotic stress; Adaptation; Listeria monocytogenes; Sécurité des aliments; Stress froid; Stress salin; Adaptation; Listeria monocytogenes.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2549.pdf

Improving simultaneously the quality and safety of cooked and peeled shrimp using a cocktail of bioprotective lactic acid bacteria
Provedor de dados: 5
Autores: Saraoui, Taous; Cornet, Josiane; Guillouet, Emilie; Pilet, Marie France; Chevalier, Frederique; Joffraud, Jean-jacques; Leroi, Francoise.
Tropical shrimp is of considerable economic importance in the word but is highly perishable due to microbial and chemical degradation. Biopreservation is a food preservation technology based on the addition of “positive” bacteria able to kill or prevent the growth of undesirable microorganisms. Two strains of lactic acid bacteria (LAB) have previously been selected for a biopreservation strategy: Lactococcus piscium CNCM I-4031, for its ability to prevent the sensory deterioration of seafood and Carnobacterium divergens V41, which inhibits growth of Listeria monocytogenes. The objective was to test the association of the two strains to improve both the quality and safety of shrimp. In a first trial, the two LAB were inoculated alone or in a cocktail in...
Tipo: Text
Palavras-chave: Carnobacterium divergens V41; Lactococcus piscium CNCM I-4031; Penaeus vannamei; Listeria monocytogenes; Biopreservation.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00353/46412/46140.pdf

Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence
Provedor de dados: 5
Autores: Saraoui, Taous; Leroi, Francoise; Chevalier, Frederique; Cappelier, Jean-michel; Passerini, Delphine; Pilet, Marie-france.
Listeria monocytogenes is a Gram-positive pathogen occurring in many refrigerated ready-to-eat foods. It is responsible for foodborne listeriosis, a rare but severe disease with a high mortality rate (20–30%). Lactococcus piscium CNCM I-4031 has the capacity to prevent the growth of L. monocytogenes in contaminated peeled and cooked shrimp and in a chemically defined medium using a cell-to-cell contact-dependent mechanism. To characterize this inhibition further, the effect of L. piscium was tested on a collection of 42 L. monocytogenes strains. All strains were inhibited but had different sensitivities. The effect of the initial concentration of the protective and the target bacteria revealed that the inhibition always occurred when L. piscium had reached...
Tipo: Text
Palavras-chave: Biopreservation; Lactococcus piscium; Listeria monocytogenes; Co-culture; Cell ratio; Scanning electron microscopy; Virulence.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00449/56065/57588.pdf

Response of Listeria monocytogenes to liquid smoke
Provedor de dados: 5
Autores: Guilbaud, Morgan; Chafsey, Ingrid; Pilet, Marie-france; Leroi, Francoise; Prevost, Herve; Hebraud, Michel; Dousset, Xavier.
Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes.   Methods and Results: Growth and survival curves were recorded in brain-heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 mu g ml(-1) phenol while a rapid decrease in cell viability occurred in the presence of 30 mu g ml(-1) phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2-h incubation with 30 mu g ml(-1) phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly...
Tipo: Text
Palavras-chave: Survival; Stress; Proteome analysis; Listeria monocytogenes; Liquid smoke; Haemolysis.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4522.pdf

Sélection et caractérisation de souches de Carnobacterium pour la biopréservation du saumon fumé
Provedor de dados: 5
Autores: Brillet, Anne.
In order to develop a biopreservation strategy in cold-smoked salmon, the inhibitory capacity of 3 bacteriocin-producing Carnobacterium strains isolated from fish have been tested towards Listeria monocytogenes strains. C. divergens V41 maintains the level of Listeria below 50 CFU/g in sterile cold-smoked salmon during the 28 days of vacuum storage at 4 and 8°C, thanks to the divercin V41 action. For its use in the product, the culture of the strain was optimized in a growth medium without animal protein. On commercial cold-smoked salmon slices, the strain inhibits slightly the endogenous microflora and doesn't modify the sensorial quality, but it produces tyramine, a biogenic amine. A non-producing tyramine mutant was obtained by chemical mutagenesis....
Tipo: Text
Palavras-chave: Tyrosine decarboxylase; Tyramine; Cold smoked salmon; Listeria monocytogenes; Divercin V41; Carnobacterium; Biopreservation; Tyrosine décarboxylase; Tyramine; Saumon fumé; Listeria monocytogenes; Divercine V41; Carnobacterium; Biopréservation.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2414.pdf

Molecular characterization of Listeria monocytogenes isolated from foods
Provedor de dados: 59
Autores: Pimenta,Fabiana Cristina; Furlanetto,Sirdéia Maura Perrone; Mayer,Leonard W.; Timenetsky,Jorge; Santos,Manoel Armando Azevedo dos.
A total of 30 strains of Listeria monocytogenes isolated from different foods (16 of differents kinds of sausage, 14 cheese,) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phosphalipase C - PI-PLC enzyme. The L. monocytogenes strains were differentiated into six ribotype classes. A total of 13 (43.3%) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard L. monocytogenes prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7%) strains when incubated at 37oC, but not at 4oC. The direct colony hybridization method for hemolysin gene detection showed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Ribotyping; Virulence; Factors; Food.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011

Cepas de Lactobacillus spp. con capacidades probióticas aisladas del tracto intestinal de terneros neonatos
Provedor de dados: 101
Autores: Sánchez,Lilian; Omura,Michele; Lucas,Adams; Pérez,Tania; Llanes,Marisleidys; Ferreira,Celia de Luce.
Se caracterizaron in vitro 17 cepas de Lactobacillus spp. aisladas del tracto intestinal de terneros neonatos, para seleccionar las mejores como candidatos probióticos y realizarles posteriormente los estudios in vivo. A todos los aislados se les realizó la prueba de la catalasa, la tinción de Gram y se estudió sus características culturales para su identificación preliminar. Se evaluó su capacidad probiótica mediante pruebas in vitro como fueron el crecimiento a diferentes temperaturas (30, 37 y 45°C), pH (2,5; 3,4; 5,4; 6,4) y capacidad de acidificación del medio de cultivo. Se preseleccionaron 10 cepas de Lactobacillus spp. que crecieron en todas las condiciones evaluadas. Se destaca el crecimiento de células viables de 6,7-10 log UFC/ml que...
Tipo: Journal article
Palavras-chave: Lactobacillus spp.; Probiótico; Salmonella sp.; Staphylococcus aureus; Escherichia coli; Listeria monocytogenes.
Ano: 2015
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000200004

Ocorrência de Listeria monocytogenes em queijo artesanal tipo coalho comercializado na cidade de Manaus-AM, Brasil
Provedor de dados: 36
Autores: Ramos,Simone de Nazaré Melo; Costa,Cristóvão Alves da.
A ocorrência de Listeria sp. em queijo artesanal tipo coalho comercializado na cidade de Manaus foi estudada. No período de março a maio de 1998 foram coletadas 58 amostras do produto em feiras e mercados localizados em seis zonas da cidade de Manaus. Em duas amostras de queijo foram identificadas duas espécies de Listeria: L. monocytogenes e L. innocua. A primeira espécie é reconhecidamente patogênica para o ser humano. Os resultados apresentados demonstraram haver risco de transmissão de enfermidades pelo consumo de queijos processados sem os devidos cuidados de higiene.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Queijo; Listeria monocytogenes; Listeria sp.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000400007

Actividad antagonista de bacterias lácticas aisladas del medio marino contra cepas de Listeria
Provedor de dados: 38
Autores: Marguet,Emilio Rogelio; Vallejo,Marisol; Sierralta Chichisola,Verónica; Quispe,Jorge León.
En el presente trabajo se estudiaron 74 bacterias lácticas para determinar su capacidad de inhibir el desarrollo de Listeria monocytogenes ATCC 7644 o L. innocua ATCC 33090. Dentro de las 7 que exhibieron actividad, 2 se seleccionaron para estudios complementarios y se identificaron por métodos fenotípicos y genotípicos como Enterococcus mundtii Tw 56 y Lactococcus lactis subsp. lactis Tw 34. Después de la neutralización con NaOH y tratamiento térmico (100 ºC y 121 ºC durante 5 min) la actividad antagonista del sobrenadante libre de células se mantuvo activa. El tratamiento con lisozima, lipasa y catalasa no afectó la actividad antimicrobiana. Sin embargo, la actividad enzimática de la tripsina suprimió la acción inhibitoria del sobrenadante, confirmando...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacterias lácticas; Listeria monocytogenes; Bacteriocinas; Ambiente marino.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572011000200008

Listeriose em rebanho de ovinos leiteiros na região serrana do Rio Grande do Sul: relato de caso
Provedor de dados: 45
Autores: Ribeiro,L.A.O.; Rodrigues,N.C.; Fallavena,L.C.B.; Oliveira,S.J.; Brito,M.A.
Relatam-se dois casos de listeriose ocorridos em ovinos leiteiros da raça Lacaune, mantidos em confinamento e alimentados com silagem de milho. O exame histológico do tecido nervoso mostrou meningoencefalite supurada, e a imunoistoquímica foi positiva para Listeria monocytogenes com isolamento da bactéria do tecido nervoso. Enfatiza-se a ocorrência dessa enfermidade em ovinos leiteiros que apresentavam lesões na cavidade oral e eram alimentados com silagem de baixa qualidade.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovino; Listeria monocytogenes; Listeriose.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300005

Antimicrobial activity of the Nisin Z producer Lactococcus lactis subsp. lactis Lc08 against Listeria monocytogenes in skim milk
Provedor de dados: 45
Autores: Perin,L.M.; Miranda,R.O.; Camargo,A.C.; Colombo,M.; Carvalho,A.F.; Nero,L.A.
The presented study aimed to verify the effect of different pH values, enzyme solutions and heat treatments on the antimicrobial activity of the bacteriocinogenic strain Lactococcus lactis subsp. lactis Lc08 and to test their antimicrobial activity against Listeria monocytogenes in reconstituted skim milk at refrigeration temperatures. This strain was previously described as a nisin Z producer and capable of inhibiting L. monocytogenes growth in in vitro tests. The antimicrobial activity of the bacteriocin cell-free supernatant of Lc08 was sensitive to enzyme treatments (except papain). The pH values and heating (65ºC for 30min, 75ºC for 15s) had no apparent effect on the antimicrobial activity of the bacteriocin produced by Lc08. Only treatment at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nisin Z; Milk; Antagonistic activity; Lactococcus lactis subsp. lactis; Listeria monocytogenes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500037

Atividade antimicrobiana de bactérias lácticas de embutidos curados frente a Listeria monocytogenes
Provedor de dados: 45
Autores: Prado,C.S.; Santos,W.L.M.; Carvalho,C.R.; Moreira,E.C.; Costa,O.
Cepas de bactérias lácticas recuperadas de 336 colônias isoladas e selecionadas foram submetidas ao teste de atividade antimicrobiana direta, que identificou as produtoras de substâncias antimicrobianas capazes de inibir in vitro o desenvolvimento de duas cepas indicadoras de Listeria monocytogenes. As 108 cepas que inibiram diretamente pelo menos uma das cepas indicadoras receberam a denominação DTEI e foram selecionadas para o teste de atividade antimicrobiana indireta contra as mesmas cepas de L. monocytogenes, assim como frente a outras cepas de bactérias lácticas de origens diversas. Essa atividade inibidora indireta foi avaliada por meio de sobrenadantes isentos de células, esterilizados por meio de microfiltração, eliminando-se os principais...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bactéria láctica; Atividade antimicrobiana; Listeria monocytogenes; Lactobacillus sp.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400022

Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization
Provedor de dados: 45
Autores: Oliveira,M.; Guerra,M.; Bernardo,F.
Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH) para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%). Por meio do protocolo FISH, observou-se que 22 silos (29,7%) se encontravam contaminados. O método FISH apresentou precisão (77,0%), especificidade (92,5%) e sensibilidade (72,7%) elevadas, sendo adequado para a análise microbiológica presuntiva de silagens, uma vez que é um método...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silage; Listeriosis; Fluorescent in situ hybridization; Listeria monocytogenes; Portugal.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100038

Influence of the cleaning system of conveyor belts on microbiological quality of poultry meat
Provedor de dados: 45
Autores: Julião,T.A.; Cortezi,A.M.; Rossi,G.A.M.; D’Abreu,L.F.; Balieiro,J.C.C.; Vidal,A.M.C.
ABSTRACT This study focused on assessing the microbiology of conveyor belts surfaces, cleaned or not with pressurized water, and verification of the influence of this process on the microbiological quality of poultry meat. A reduction on mesophilic, psychrotrophic and Enterobacteriaceae counts (P&lt;0.05) on dry conveyor belts compared to wet ones was observed. For the chicken leg (consisting of drumstick and thigh) samples, no statistically significant differences were detected on counts of indicators of microorganisms on poultry legs (composed by drumstick and thigh). For poultry meat conducted on wet or dry conveyor belts, 99% and 86%, were positive for Listeria spp, respectively. Only one sample of chicken leg was positive for presence of L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enterobacteriaceae; Listeria monocytogenes; Food microbiology; Staphylococcus spp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401325

Effect of eugenol on growth and listeriolysin o production by Listeria monocytogenes
Provedor de dados: 52
Autores: Filgueiras,Cristina Tostes; Vanetti,Maria Cristina Dantas.
The inhibitory effect of eugenol, a naturally occurring compound mainly present in the essential oil fraction of cloves, was studied on the growth and listeriolysin O (LLO) production by Listeria monocytogenes. Potassium efflux from cells promoted by eugenol was also determined after 24 h incubation in phosphate buffered saline. Eugenol promoted a delay on the growth of L. monocytogenes at concentrations of 100, 300 and 500 µg mL-1and above 800 µg mL-1 the effect was bactericidal. Production of LLO by L. monocytogenes in the presence of eugenol was reduced 80-100%. An accumulation of external K+ was observed above 300 µg mL-1 of eugenol which indicated that the cell membrane was affected. The results showed the effectiveness of eugenol in controlling...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eugenol; Listeria monocytogenes; Antilisteric; LLO.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400008

Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil
Provedor de dados: 55
Autores: Almeida,Rosana Macedo de; Barbosa,André Victor; Lisbôa,Rodrigo de Castro; Santos,André Felipe das Mercês; Hofer,Ernesto; Vallim,Deyse Christina; Hofer,Cristina Barroso.
ABSTRACT The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Listeriosis; Virulence; Food; PFGE; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300282

Antimicrobial activity of some of the south-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila
Provedor de dados: 58
Autores: Indu,M.N.; Hatha,A.A.M.; Abirosh,C.; Harsha,U.; Vivekanandan,G.
Antibacterial activity of extracts of Allium sativum (garlic), Myristica fragrans (nutmeg), Zingiber officinale (ginger), Allium cepa (onion) and Piper nigrum (pepper) has been evaluated against 20 different serogroups of Escherichia coli, 8 serotypes of Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Garlic extract showed excellent antibacterial activity against all the test organisms, except L. monocytogenes. Nutmeg showed good anti-listerial activity, although activity against E. coli and Salmonella were serotype dependent. Both garlic and nutmeg extracts were effective against A. hydrophila. Extracts of ginger showed inhibitory activity against two serogroups of E. coli: as O8 (enterotoxigenic E. coli) and O88 only. Extracts of onion and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spices extract; Antibacterial activity; Escherichia coli; Salmonella; Listeria monocytogenes; Aeromonas hydrophila.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200011

Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged brazilian meat and meat products
Provedor de dados: 58
Autores: Martinis,Elaine C.P. De; Públio,Márcia R.P.; Santarosa,Priscila R.; Freitas,Flávia Z.
Twenty samples of Brazilian meat and meat products were screened by the agar overlay method for bacteriocin-producing lactic acid bacteria, using Lactobacillus sake ATCC 15521 as indicator strain. Based on Gram staining, KOH reaction, catalase test and fermentation of 49 carbohydrates (API 50 CH), three out of seven isolates with confirmed antagonist properties were identified as Lactobacillus curvatus, one as Leuconostoc mesenteroides and one as Leuconostoc sp. Two isolates could not be properly identified using these tests. The inhibitors produced by these strains were sensitive to proteases. Inhibition due to lytic bacteriophages was ruled out, so the isolates were classified as bacteriocin-producing lactic acid bacteria. Four of them presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactic acid bacteria; Meat; Bacteriocins; Biopreservation; Listeria monocytogenes.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100008

Effect of lactic acid bacteria on extention of shelf life and growth of Listeria monocytogenes in beef steaks stored in CO2- rich atmosphere
Provedor de dados: 58
Autores: Djenane,Djamel; Martínez,Luis; Blanco,Domingo; Yangüela,Javier; Beltrán,José A.; Roncalés,Pedro.
Beef steaks were inoculated with one or other of two protective strains of lactic acid bacteria (bacteriocinogenic Lactobacillus sakei CTC 372 or the uncharacterized Lactobacillus CTC 711), and stored under modified atmospheres (20-40% CO2). Inoculation of meat with LAB inhibited growth of the spoilage bacteria. Neither CO2 in the pack atmosphere or inoculation with protective strains, nor a combination of both, affected formation of metmyoglobin or the development of off-odours. The formation of metmyoglobin in meat pigments and the sensory odour scores were compatible to those of fresh meat which had not undergone either oxidative deterioration or microbial spoilage. Listeria monocytogenes was inhibited in broth by meat surface microbiota containing the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fresh meat; Modified atmosphere; Preservation; Lactic acid bacteria; Spoilage; Listeria monocytogenes.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400018

Inhibitory activity of Syzygium aromaticum and Cymbopogon citratus (DC.) Stapf. essential oils against Listeria monocytogenes inoculated in bovine ground meat
Provedor de dados: 58
Autores: Oliveira,Thales Leandro Coutinho de; Cardoso,Maria das Graças; Soares,Rodrigo de Araújo; Ramos,Eduardo Mendes; Piccoli,Roberta Hilsdorf; Tebaldi,Victor Maximiliano Reis.
This research evaluated the antimicrobial effect of the clove (Syzygium aromaticum) and lemongrass (Cymbopogon citratus (DC.) Stapf.) essential oils (EOs) against Listeria monocytogenes ATCC 19117 growth added to bovine ground meat stored under refrigeration (5 ± 2 °C) for three days. The EOs, extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS), were tested in vitro using an agar well diffusion methodology for determination of Minimum Inhibitory Concentration (MIC). The MIC concentrations for both essential oils on culture tested of L. monocytogenes were 1.56%. The EOs concentrations applied in contaminated ground beef were 1.56, 3.125 and 6.25% (w/v) based on MIC levels and possible activity reductions by food...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clove; Lemongrass; Food safety; Listeria monocytogenes; Sensorial.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200003

Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system
Provedor de dados: 58
Autores: Rabiey,Soghra; Hosseini,Hedayat; Rezaei,Masoud.
This study was conducted to evaluate the antibacterial effect of Carum copticum essential oil (Ajowan EO) against Listeria monocytogenes in fish model system. Ajowan EO chemical composition was determined by gas chromatography/mass spectral analysis and the highest concentration of Carum copticum essential oil without any significant changes on sensory properties of kutum fish (Rutilus frisii kutum) was assigned. Then the inhibitory effect of Ajowan EO at different concentrations in presence of salt and smoke component was tested on L. monocytogenes growth in fish peptone broth (FPB), kutum broth and cold smoked kutum broth at 4 ºC for 12 days. Ajowan EO completely decreased the number of L. monocytogenes in FPB after 12 days of storage, however,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Carum copticum; Listeria monocytogenes; Fish model systems; Hurdle technology; Rutilus frisiikatum.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100013

Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
Provedor de dados: 58
Autores: Lee,H.Y.; Chai,L.C.; Pui,C.F.; Mustafa,S.; Cheah,Y.K.; Nishibuchi,M.; Radu,S.
Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1-10% (w/v) and incubated at different temperature of 4 ºC, 30 ºC and 45 ºC for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4 ºC. The formation of biofilm...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Biofilm; Sodium chloride; Temperature; Microtiter plate.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100007

Epidemiological Survey of Listeria monocytogenes in a gravlax salmon processing line
Provedor de dados: 58
Autores: Cruz,C.D.; Silvestre,F.A.; Kinoshita,E.M.; Landgraf,M.; Franco,B.D.G.M.; Destro,M.T.
Listeria monocytogenes is a cause of concern to food industries, mainly for those producing ready-to-eat (RTE) products. This microorganism can survive processing steps such as curing and cold smoking and is capable of growing under refrigeration temperatures. Its presence in RTE fish products with extended shelf life may be a risk to the susceptible population. One example of such a product is gravlax salmon; a refrigerated fish product not exposed to listericidal processes and was the subject of this study. In order to evaluate the incidence and dissemination of L. monocytogenes 415 samples were collected at different steps of a gravlax salmon processing line in São Paulo state, Brazil. L. monocytogenes was confirmed in salmon samples (41%), food contact...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Gravlax salmon; PFGE.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200032

Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential
Provedor de dados: 58
Autores: Oliveira,Maíra Maciel Mattos de; Brugnera,Danilo Florisvaldo; Alves,Eduardo; Piccoli,Roberta Hilsdorf.
An experimental model was proposed to study biofilm formation by Listeria monocytogenes ATCC 19117 on AISI 304 (#4) stainless steel surface and biotransfer potential during this process. In this model, biofilm formation was conducted on the surface of stainless steel coupons, set on a stainless steel base with 4 divisions, each one supporting 21 coupons. Trypic Soy Broth was used as bacterial growth substrate, with incubation at 37 ºC and stirring of 50 rpm. The number of adhered cells was determined after 3, 48, 96, 144, 192 and 240 hours of biofilm formation and biotransfer potential from 96 hours. Stainless steel coupons were submitted to Scanning Electron Microscopy (SEM) after 3, 144 and 240 hours. Based on the number of adhered cells and SEM, it was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Biofilm; Biotransfer potential.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100016

Occurrence and antimicrobial resistance patterns of Listeria monocytogenes isolated from vegetables
Provedor de dados: 58
Autores: Byrne,Vanessa de Vasconcelos; Hofer,Ernesto; Vallim,Deyse Christina; Almeida,Rogeria Comastri de Castro.
Abstract Although the consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Among the food-borne pathogens that contaminate vegetables is Listeria monocytogenes, a ubiquitous organism that exhibits the ability to survive and multiply at refrigerated temperatures. This study aimed to evaluate the occurrence of L. monocytogenes in vegetables as well as the antimicrobial resistance of isolates. The results showed that 3.03% of samples were contaminated with L. monocytogenes, comprising 2.22% of raw vegetables and 5.56% of ready-to-eat vegetables. Multiplex PCR confirmed the virulence potential of the isolates. Antimicrobial resistance profiling showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetables; Food safety; Listeria monocytogenes; Antimicrobial resistance.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200438

Growth, viability and architecture of biofilms of Listeria monocytogenes formed on abiotic surfaces
Provedor de dados: 58
Autores: Reis-Teixeira,Fernanda Barbosa dos; Alves,Virgínia Farias; Martinis,Elaine Cristina Pereira de.
Abstract The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion. In this study, biofilm populations of two strains of L. monocytogenes (serotypes 1/2a and 4b) on stainless steel coupons and glass were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Abiotic surfaces; Persistence; Biofilms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300587

Antibacterial efficacy of Nisin, Pediocin 34 and Enterocin FH99 against Listeria monocytogenes and cross resistance of its bacteriocin resistant variants to common food preservatives
Provedor de dados: 58
Autores: Kaur,G.; Singh,T.P.; Malik,R.K.
Antilisterial efficiency of three bacteriocins, viz, Nisin, Pediocin 34 and Enterocin FH99 was tested individually and in combination against Listeria mononcytogenes ATCC 53135. A greater antibacterial effect was observed when the bacteriocins were combined in pairs, indicating that the use of more than one LAB bacteriocin in combination have a higher antibacterial action than when used individually. Variants of Listeria monocytogenes ATCC 53135 resistant to Nisin, Pediocin 34 and Enterocin FH99 were developed. Bacteriocin cross-resistance of wild type and their corresponding resistant variants were assessed and results showed that resistance to a bacteriocin may extend to other bacteriocins within the same class. Resistance to Pediocin 34 conferred cross...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Nisin; Pedioin 34; Enterocin FH99; Cross resistance.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100009

Screening for antimicrobial activity among bacteria isolated from the Amazon Basin
Provedor de dados: 58
Autores: Motta,Amanda S.; Cladera-Olivera,Florencia; Brandelli,Adriano.
Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml-1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial activity; Listeria monocytogenes; Bacteriocins; Amazon Basin.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300007

Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts
Provedor de dados: 58
Autores: Oladunjoye,Adebola O.; Singh,Suren; Ijabadeniyi,Oluwatosin A.
ABSTRACT The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000 UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5 g/100 mL each) on fresh-cut tomato previously inoculated with 108 CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200 ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25 °C) and examined at 0, 24, 48 and 72 h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Nisin; Fresh produce; Sodium citrate; Sodium acetate; Quality parameters.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300757

Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil
Provedor de dados: 58
Autores: Headley,Selwyn Arlington; Bodnar,Lívia; Fritzen,Juliana T.T.; Bronkhorst,Dalton Evert; Alfieri,Alice Fernandes; Okano,Werner; Alfieri,Amauri Alcindo.
Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brainstem dysfunctions; Rhombencephalitis; Neuropathology; Listeriolysin gene; Epidemiology; Listeria monocytogenes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300036

Prevalence and quantification of Listeria monocytogenes in beef offal at retail level in Selangor, Malaysia
Provedor de dados: 58
Autores: Kuan,Chee Hao; Wong,Woan Chwen; Pui,Chai Fung; Mahyudin,Nor Ainy; Tang,John Yew Huat; Nishibuchi,Mitsuaki; Radu,Son.
A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method was employed in this study. It was found that L. monocytogenes detected in 33.33% of the beef offal samples. The prevalence of L. monocytogenes in beef offal purchased from wet markets A, B, and C were 22.73%, 37.50% and 41.18% respectively. The density of L. monocytogenes in all the samples ranged from&lt;3upto&amp;gt; 2,400 MPN/g. The findings in this study indicate that beef offal can be a potential vehicle of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Most probable number (MPN); Polymerase chain reaction (PCR); Beef offal; Prevalence.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400020

Hazards in non-pasteurized milk on retail sale in Brazil: prevalence of Salmonella spp, Listeria monocytogenes and chemical residues
Provedor de dados: 58
Autores: Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Beloti,Vanerli; Barros,Marcia A.F.; Netto,Daisy Pontes; Pinto,José Paes A.N.; Andrade,Nélio José de; Silva,Wladimir P.; Franco,Bernadette D.G.M.
Fluctuations in the Brazilian milk market force small milk producers to find temporary trade alternatives, which include selling raw milk to people who prefer this type of milk rather than heat-processed milk. Considering the importance of these small milk producers to the market and the well-known health risks associated to consumption of raw milk, this study evaluated the microbiological quality and the presence of Listeria monocytogenes, Salmonella spp., chlorine, antimicrobials and insecticides (organophosphates and carbamates) in raw milk produced in 210 small and medium farms located in four important milk-producing Brazilian states (Paraná, São Paulo, Minas Gerais and Rio Grande do Sul). In 66% of the selected farms the milking was manual. In 33% of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hazards; Raw milk; Salmonella; Listeria monocytogenes; Hygiene indicators; Insecticides; Antimicrobials.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200007

Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil
Provedor de dados: 58
Autores: Lemes-Marques,Eneida Gonçalves; Cruz,Cristina Durante; Destro,Maria Teresa.
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Serotypes; Antibiotic resistance; Genotypic characterization; Listeriosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019

Antibacterial efficacy of nisin, bacteriophage P100 and sodium lactate against Listeria monocytogenes in ready-to-eat sliced pork ham
Provedor de dados: 58
Autores: Figueiredo,Ana Cláudia L.; Almeida,Rogeria C.C.
ABSTRACT The effectiveness of bacteriophage P100, nisin and sodium lactate, individually and in combination, in inhibiting Listeria monocytogenes in ready-to-eat pork ham slices was assessed. The antimicrobials were applied to the surfaces of ready-to-eat pork ham slices, which were inoculated with a mixture of L. monocytogenes. Among the individual antimicrobial treatments, bacteriophage P100 was the most effective, decreasing L. monocytogenes to undetectable levels at zero and 72 h post-infection. Sodium lactate was the least effective treatment. Treatment with nisin at zero h significantly reduced initial cell density (p &lt; 0.05). However, this pattern was not observed at 72 h of storage. A significant difference (p &lt; 0.05) existed between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobials; Food safety; Meat products; Listeria monocytogenes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400724

Quantitative evaluation of Listeria monocytogenes in fresh and processed surubim fish (Pseudoplatystoma sp)
Provedor de dados: 58
Autores: Souza,V.M. de; Alves,V.F.; Destro,M.T.; De Martinis,E.C.P.
L. monocytogenes is a foodborne psychrotrophic bacterial pathogen of special importance for minimally processed foods. In this work, it was enumerated in samples of surubim fish by MPN technique. The population of L. monocytogenes was estimated as &lt; 0.012 MPN/cm² in fresh and &lt; 0.03 MPN/g in minimally processed fish.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; MPN; Fish; Surubim.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300023

Symptomatic hydrocephalus in a newborn infected with listeria monocytogenes
Provedor de dados: 58
Autores: Laciar,Analía L.; Hasuoka,Raúl P.; Correa,Silvia M.; Miranda,Alicia M.; Centorbi,Olga N.P.
Central nervous system infections caused by Listeria monocytogenes produce a wide range of clinical symptoms which include cerebral abscesses, meningitis and nonmeningitic parenchymal cerebritis. A case study is presented of early listeriosis with signs of meningitis accompanied with septicemia and complicated with severe hydrocephalus.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Listeria monocytogenes; Meningitis; Septicemia; Hydrocephalus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100003

Inhibition of listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated nisin
Provedor de dados: 58
Autores: Malheiros,Patrícia da Silva; Daroit,Daniel Joner; Brandelli,Adriano.
The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.1 mg/mL and 0.25 mg/mL) and nanovesicle-encapsulated nisin (0.25 mg/mL) were applied onto the surface of cheese samples, and L. monocytogenes was inoculated before incubation at 6-8°C for 28 days. A bactericidal effect was observed with 0.25 mg/mL free nisin; a bacteriostatic effect was observed for liposome-encapsulated nisin and 0.1 mg/mL free nisin. Free nisin was more efficient than nisin-loaded liposomes in controlling L. monocytogenes. Possible reasons for this behavior, and also the significance of nisin to soft cheeses are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nisin; Liposome; Encapsulation; Cheese; Listeria monocytogenes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400024

Behaviour of L. monocytogenes in sliced, vacuum-packed mortadella
Provedor de dados: 58
Autores: Bersot,Luciano dos Santos; Gillio,Cíntia; Tavolaro,Paula; Landgraf,Mariza; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa.
This study evaluated the growth of naturally occurring L. monocytogenes in sliced, vacuum-packed mortadella samples during storage at 5ºC until the expiration date. Tukey's test indicated that counts of L. monocytogenes on 0, 10, 20, 30 and 40 days of storage were significantly different (p&lt;0.05), indicating growth during shelf life. In three trials, the mean increase was 1.72 log cycles. Vacuum packing and storage under refrigeration were not effective in controlling the growth of L. monocytogenes in sliced mortadella, indicating that good manufacturing practices and implemented HACCP programs are essential to assure safety of this product.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Growth; Sliced mortadella; Vacuum-packing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300020

Molecular analysis of the iap gene of Listeria monocytogenes isolated from cheeses in Rio Grande do Sul, Brazil
Provedor de dados: 58
Autores: Mello,Jozi Fagundes de; Einsfeldt,Karen; Frazzon,Ana Paula Guedes; Costa,Marisa da; Frazzon,Jeverson.
The polymorphic region sequences in the iap gene were analyzed in 25 strains of Listeria monocytogenes isolated from cheeses in the state of Rio Grande do Sul, and compared with reference strains. This investigation distinguished two clusters of L. monocytogenes: I (20 strains) and II (5 strains).
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Iap gene; Cheese; TN amino acids.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100033

Antimicrobial effect of copper surfaces on bacteria isolated from poultry meat
Provedor de dados: 58
Autores: Parra,Angel; Toro,Magaly; Jacob,Ricardo; Navarrete,Paola; Troncoso,Miriam; Figueroa,Guillermo; Reyes-Jara,Angélica.
Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens – Salmonella enterica and Listeria monocytogenes – and a poultry native microbiota bacterial species – Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial copper-alloys; Foodborne pathogens; Salmonella; Listeria monocytogenes; Poultry meat.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500113

Antibacterial activity of bacteriocin-like substance P34 on Listeria monocytogenes in chicken sausage
Provedor de dados: 58
Autores: Sant'Anna,Voltaire; Quadros,Deoni A.F.; Motta,Amanda S.; Brandelli,Adriano.
The antimicrobial activity of the bacteriocin-like substance (BLS) P34 against Listeria monocytogenes was investigated in chicken sausage. The BLS was applied to chicken sausages (256 AU g-1) previously inoculated with a suspension of 10² cfu g-1 of L. monocytogenes. BLS P34 inhibited the indicator microorganism in situ in all incubation times for up to 10 days at 5 °C. The effectiveness of BLS P34 was increased when it was added in combination with nisin. The bacteriocin was also tested in natural eatable natural bovine wrapping (salty semi-dried tripe) against the same indicator microorganism, also showing inhibitory capability in vitro. BLS P34 showed potential to control L. monocytogenes in refrigerated meat products.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bacteriocin; Chilled chicken sausage; Listeria monocytogenes; Biopreservation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400019

Survival of Listeria monocytogenes in low acid italian sausage produced under brazilian conditions
Provedor de dados: 58
Autores: Degenhardt,Roberto; Sant'Anna,Ernani S.
Dry sausages have been considered ready-to-eat products with low risk of causing listeriosis due to the hurdles created during the manufacturing process such as low pH and a w, high salt concentration and presence of lactic acid bacteria (LAB). However, several studies have detected survival of Listeria monocytogenes in these products and also shown that process parameters, LAB and L. monocytogenes strains directly influence the results. In this work, survival of the pathogen in sausages prepared with three different formulations (one standard formulation, one formulation added of Lactobacillus plantarum and one added of 2% sodium lactate), using the manufacturing process usually employed in Brazil, was evaluated. Naturally contaminated sausages presented...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Lactobacillus plantarum; Fermented dry sausage; Survival; Brazilian salami.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200024

Evaluation of bacteriocin-producing Lactobacillus sakei 1 against Listeria monocytogenes 1/2a growth and haemolytic activity
Provedor de dados: 58
Autores: Martinez,Rafael C.R.; De Martinis,Elaine C.P.
Bacteriocin-producing Lactobacillus sakei 1 was cultivated in Brain-Heart Infusion broth (24 h at 25ºC). The culture supernatant was neutralized, filter sterilized and used to test the activity of bacteriocin against Listeria monocytogenes 1/2a, at 8ºC and 15ºC. Non-bacteriocinogenic Lactobacillus sakei ATCC 15521 was used as a negative control. L. monocytogenes 1/2a was inoculated in culture supernatant medium from L. sakei 1 and L. sakei ATCC 15521 and the listerial populations were determined after 0, 5 and 10 days. The bacteriocin production was quantified as arbitrary units per mL (AU/mL) using agar antagonism test. Additionally, to investigate if L. monocytogenes virulence pattern could be changed after bactericion exposure, the ability of L....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Lactobacillus sakei; Bacteriocins; Virulence; Resistance.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100016

Expression of virulence genes by Listeria monocytogenes J0161 in natural environment
Provedor de dados: 58
Autores: Tirumalai,Prem Saran; Prakash,Soam.
Majority of studies concerning the gene expression of Listeria monocytogenes have been done on pure culture states. Our objective was to study L.monocytogenes in a co-cultured state and to understand if microbes in their natural state of existence are different in their expression than that of the purely cultured lab grown forms. For a long period discussions have been on the expression of prfA, (which is a virulence gene regulator) in a mammalian host and its role in causing the switch from a saprophytic to pathogenic form of L.monocytogenes. We, in this paper for the first time report the expression of prfA and other virulence genes by L.monocytogenes under different extracellular conditions, and also as a pure culture biofilms, that is different from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; PrfA; Virulence; Microarray; Biofilms; Co-culture; Gene Expression.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200050

Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE
Provedor de dados: 58
Autores: von Laer,Ana Eucares; Lima,Andréia Saldanha de; Trindade,Paula dos Santos; Andriguetto,Cristiano; Destro,Maria Teresa; Silva,Wladimir Padilha da.
Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; PFGE; Serotyping; Persistent strains.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300021

Microbiological quality and safety assessment in the production of moderate and high humidity cheeses
Provedor de dados: 65
Autores: Prates,Denise da Fontoura; Würfel,Simone Rauber; Goldbeck,Júlia Coswig; Lima,Andreia Saldanha de; Lopes,Graciela Volz; Silva,Wladimir Padilha da.
ABSTRACT: Moderate and high humidity cheeses are described as important vehicles of pathogens in many foodborne diseases outbreaks. Microbial contamination can occur in raw material or in the different steps of the product processing due to inadequate hygiene practices. Thus, the aim of this study was to evaluate the microbiological quality and safety in the production of moderate and high humidity cheese. Samples from raw milk, handlers’ hands surface, final product were collected in three cheese manufacturing plants located in southern Brazil, with different levels of sanitary control. Effectiveness of milk pasteurization was also evaluated. Thermotolerant coliforms, coagulase-positive staphylococci (CPS), Salmonella spp., and Listeria monocytogenes were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Salmonella spp.; Thermotolerant coliforms; Coagulase-positive staphylococci; Food safety.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100781

Qualidade bacteriológica de queijos artesanais comercializados em estradas do litoral norte do Rio Grande do Sul, Brasil
Provedor de dados: 65
Autores: Zaffari,Cristina Bergman; Mello,Jozi Fagundes; Costa,Marisa da.
O consumo de queijo artesanal, vendido em estabelecimentos de beira de estrada, é comum no Estado do Rio Grande do Sul. Geralmente estes produtos não são fabricados em acordo com as boas normas de fabricação e podem constituir perigo à saúde do consumidor. Objetivou-se, com o presente trabalho, verificar a qualidade bacteriológica de queijos artesanais comercializados em estradas litorâneas por meio da contagem de coliformes e pesquisa de Listeria spp. e Brucella spp. Foram analisados 80 queijos, sendo 62 do tipo Colonial, dez do tipo Provolone, seis do tipo Ricota e dois do tipo Caccio Cavallo. No momento da coleta, 71% das amostras não estavam sob refrigeração. Todas as amostras apresentaram contagens de coliformes totais e, destas, 62 foram testadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caccio Cavallo; Colonial; Provolone; Ricota; Coliformes; Listeria monocytogenes; Brucella spp.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000300040

Desenvolvimento de material de referência para microbiologia de alimentos contendo Listeria monocytogenes em matriz queijo
Provedor de dados: 65
Autores: Brandão,Marcelo Luiz Lima; Costa,Juliana de Castro Beltrão da; Farias,Felipe Miceli de; Rosas,Carla de Oliveira; Bricio,Silvia Maria Lopes; Nascimento,Janaína dos Santos; Leite,Paola Cardarelli.
A NBR ISO/IEC 17025:2005, uma das normas mais utilizadas em laboratórios de ensaio, descreve como um de seus critérios para a garantia da qualidade dos resultados analíticos a participação periódica dos laboratórios em ensaios de proficiência (EP). Os analitos, utilizados nos EP são materiais de referência (MR) provenientes de um mesmo lote, e devem apresentar características de homogeneidade e estabilidade. O objetivo deste estudo foi produzir um MR qualitativo destinado ao ensaio de pesquisa de Listeria monocytogenes em matriz queijo pela técnica de liofilização. Para a produção do MR, foi utilizado como matriz o queijo Minas frescal (QMF) ultrafiltrado. A matriz foi distribuída em frascos, contaminada com a bactéria alvo e submetida à liofilização,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Material de referência; Queijo; Listeria monocytogenes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000028

Etiologic diagnosis of bovine infectious abortion by PCR
Provedor de dados: 65
Autores: Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima.
Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028

Surto de listeriose sistêmica em chinchilas
Provedor de dados: 65
Autores: Kirinus,Jackeline Karsten; Krewer,Carina; Zeni,Diego; Monego,Fernanda; Silva,Marcia Cristina da; Kommers,Glaucia Denise; Vargas,Agueda Castagna de.
A listeriose é uma doença infecciosa que afeta uma grande variedade de espécies animais, causando septicemia, encefalite e aborto. As chinchilas são os animais mais susceptíveis à infecção sistêmica por Listeria monocytogenes. Este relato descreve um surto de listeriose sistêmica em uma criação de chinchilas da região central do Estado do Rio Grande do Sul, onde cerca de 16% das chinchilas morreram. Na necropsia, havia múltiplos focos brancos, pequenos e de distribuição aleatória nas superfícies capsular e de corte do fígado e aumento de volume do linfonodo hepático. Histologicamente, observaram-se hepatite necrossupurativa e linfadenite supurativa multifocais, com numerosos bacilos intralesionais. L. monocytogenes foi o agente etiológico do surto de...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Doenças de chinchilas; Listeria monocytogenes; Bacteriologia; Patologia.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000300033

Antimicrobial activity of autoclaved and non autoclaved copaiba oil on Listeria monocytogenes
Provedor de dados: 65
Autores: Pieri,Fábio Alessandro; José,Raphaela Mansur; Galvão,Newton Nascentes; Nero,Luis Augusto; Moreira,Maria Aparecida Scatamburlo.
The aim of this study was to evaluate the antimicrobial effect of different copaiba oil concentrations against the growth of Listeria monocytogenes, and analyze differences in inhibition of microorganisms with autoclaved and non autoclaved oil. This study provided an agar diffusion test with six isolates of bacteria and six different concentrations of autoclaved or non autoclaved copaiba oil and a negative control. The results showed sensitivity of five L. monocytogenes isolates related to the 10% autoclaved solution of copaiba oil. Four strains also showed sensitivity to the 5% autoclaved solution and one to 2.5% autoclaved solution. The 10% non autoclaved oil solution showed growth inhibition only for two strains. These results had pointed the 10%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytotherapics; Copaifera; Antimicrobial drugs; Listeria monocytogenes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000800020

Listeria spp. no processamento de lingüiça frescal em frigoríficos de Pelotas, RS, Brasil
Provedor de dados: 65
Autores: Silva,Wladimir Padilha da; Lima,Andréia Saldanha de; Gandra,Eliezer Ávila; Araújo,Márcia Ribeiro de; Macedo,Márcia Raquel Pegoraro de; Duval,Eduarda Hallal.
Alimentos intensamente manipulados, como as lingüiças mistas do tipo frescal são freqüentemente responsáveis pela veiculação de enfermidades transmitidas por alimentos. Devido ao risco à saúde pública que a bactéria Listeria monocytogenes representa, este trabalho teve como objetivo estudar a presença de Listeria spp., em especial de L. monocytogenes, durante o processamento de lingüiças mistas do tipo frescal, em três frigoríficos com inspeção sanitária estadual, em Pelotas-RS. Para isso, analisou-se a matéria-prima utilizada no preparo da lingüiça, os equipamentos da linha de processamento e o produto final. Isolou-se Listeria spp. em 100% das 41 amostras analisadas, nos 3 estabelecimentos estudados. Dentre as diferentes espécies, L. innocua foi aquela...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria spp; Listeria monocytogenes; Lingüiça frescal; Planta de processamento.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300039

Ocorrência e diferenciação de espécies de Listeria spp. em salsichas tipo hot dog a granel e em amostras de carne moída bovina comercializadas no Distrito Federal
Provedor de dados: 65
Autores: Andrade,Rafael Rocha de; Silva,Patrícia Helena Caldeira da; Souza,Nara Rúbia; Murata,Luci Sayori; Gonçalves,Vitor Salvador Picão; Santana,Angela Patrícia.
Listeria monocytogenes é um patógeno relevante veiculado por alimentos. Sua identificação precisa é importante para a correta determinação do risco associado à ingestão do alimento. O objetivo deste trabalho foi verificar a ocorrência das espécies de Listeria spp. em amostras de salsichas tipo hot dog e carne moída bovina, comercializadas a granel no Distrito Federal. Foram analisadas 162 amostras, sendo 127 de salsichas tipo hot dog e 35 amostras de carne moída bovina. O isolamento e a identificação do gênero foram feitos por metodologia convencional e a distinção das espécies foi verificada por kit bioquímico específico (API-Listeria®) e por análise de restrição de fragmentos da reação em cadeia da polimerase (RFLP-PCR) do gene 23 rRNA. Foram isoladas 26...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Listeria monocytogenes; Produtos cárneos; PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000100024