Consulta: subjectFacets:"Live-imaging"
Registros recuperados: 2
Data/hora: 10/06/2024 05:37:34

Spatial compartmentalization of free radical formation and mitochondrial heterogeneity in bivalve gills revealed by live-imaging techniques
Provedor de dados: 5
Autores: Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris.
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas...
Tipo: Text
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf

Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
Provedor de dados: 5
Autores: Rivera-ingraham, Georgina A.; Nommick, Aude; Blondeau-bidet, Eva; Ladurner, Peter; Lignot, Jehan-herve.
In the context of global change, there is an urgent need for researchers in conservation physiology to understand the physiological mechanisms leading to the acquisition of stress acclimation phenotypes. Intertidal organisms continuously cope with drastic changes in their environmental conditions, making them outstanding models for the study of physiological acclimation. As the implementation of such processes usually comes at a high bioenergetic cost, a mitochondrial/oxidative stress approach emerges as the most relevant approach when seeking to analyze whole-animal responses. Here we use the intertidal flatworm Macrostomum lignano to analyze the bioenergetics of salinity acclimation and its consequences in terms of reactive oxygen/nitrogen species...
Tipo: Text
Palavras-chave: Energetic balance; Flatworms; Live-imaging; Mitochondrial membrane potential; Osmoregulation; RNS; ROS.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00625/73743/74411.pdf