Consulta: subjectFacets:"MALDI-TOF MS"
Registros recuperados: 6
Data/hora: 06/06/2024 11:38:48

A MALDI-TOF MS database for fast identification of Vibrio spp. potentially pathogenic to marine mollusks
Provedor de dados: 5
Autores: Moussa, M.; Cauvin, E.; Le Piouffle, A.; Lucas, O.; Bidault, Adeline; Paillard, Christine; Benoit, F.; Thuillier, B.; Treilles, M.; Travers, Marie-agnes; Garcia, Celine.
In mollusk aquaculture, a large number of Vibrio species are considered major pathogens. Conventional methods based on DNA amplification and sequencing used to accurately identify Vibrio species are unsuitable for monitoring programs because they are time-consuming and expensive. The aim of this study was, therefore, to develop the MALDI-TOF MS method in order to establish a rapid identification technique for a large panel of Vibrio species. We created the EnviBase containing 120 main spectra projections (MSP) of the Vibrio species that are potentially responsible for mollusk diseases, comprising 25 species: V. aestuarianus, V. cortegadensis, V. tapetis and species belonging to the Coralliilyticus, Harveyi, Mediterranei, and Orientalis clades. Each MSP was...
Tipo: Text
Palavras-chave: Vibrio; MALDI-TOF MS; Database; Marine mollusk pathogens.
Ano: 2021
URL: https://archimer.ifremer.fr/doc/00680/79224/81732.pdf

Bacteremia due to Moraxella osloensis: a case report and literature review
Provedor de dados: 55
Autores: Maruyama,Yuta; Shigemura,Tomonari; Aoyama,Koki; Nagano,Noriyuki; Nakazawa,Yozo.
ABSTRACT Herein we report the case of a 10-year-old boy with an autosomal mosaic mutation who developed bacteremia. The causative agent was identified as Moraxella osloensis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene sequencing. In the pediatric population, there have been 13 case reports of infection attributed to M. osloensis and this is the fifth reported case of pediatric bacteremia due to M. osloensis. After Moraxella species infection was confirmed, the patient recovered with appropriate antimicrobial therapy. It is important to consider that M. osloensis can cause serious infections, such as bacteremia, in otherwise healthy children.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Moraxella osloensis; Bacteremia; MALDI-TOF MS; 16S rRNA gene sequencing.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100060

Co-transmission of Rahnella aquatilis between hospitalized patients
Provedor de dados: 55
Autores: Martins,Willames; Carvalhaes,Cecília Godoy; Cayô,Rodrigo; Gales,Ana Cristina; Pignatari,Antonio Carlos.
ABSTRACT Rahnella aquatilis is an environmental Gram-negative bacillus that is rarely reported as human pathogen, being mainly associated with infections in immunocompromised patients. Herein we describe two cases ofR. aquatilis isolates recovered from endotracheal aspirate cultures of different patients in a tertiary hospital located in the city of São Paulo, Brazil. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rDNA gene sequencing were performed to confirm bacterial identification after the isolates being erroneously identified as Pantoea spp. by automated system. Both isolates showed the same PFGE pattern and presented the ß-lactamase encoding geneblaRAHN-1, responsible for resistance to cephalothin. The isolates...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Rahnella spp.; Bacteria identification; MALDI-TOF MS; Environmental bacteria.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600648

Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection
Provedor de dados: 58
Autores: Lima-Neto,Reginaldo; Santos,Cledir; Lima,Nelson; Sampaio,Paula; Pais,Célia; Neves,Rejane P.
Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BRCs; Candida; Clinical yeasts; Culture collections; MALDI-TOF MS.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200020

Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics
Provedor de dados: 58
Autores: Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Javoreková,Soňa.
ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptomyces; 16S rRNA; MALDI-TOF MS; Tetrangomycin; Factumycin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400816

Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa
Provedor de dados: 60
Autores: Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Císarová,Miroslava; Javoreková,Soňa.
In the present study we reported the antimicrobial activity of actinomycetes isolated from aridic soil sample collected in Karoo, South Africa. Eighty-six actinomycete strains were isolated and purified, out of them thirty-four morphologically different strains were tested for antimicrobial activity. Among 35 isolates, 10 (28.57%) showed both antibacterial and antifungal activity. The ethyl acetate extract of strain KRG-1 showed the strongest antimicrobial activity and therefore was selected for further investigation. The almost complete nucleotide sequence of the 16S rRNA gene as well as distinctive matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) profile of whole-cell proteins acquired for strain KRG-1 led to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Actinomycin/antimicrobial activity; Streptomyces; Soil; 16S rRNA; MALDI-TOF MS.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100594