Consulta: subjectFacets:"MAT"
Registros recuperados: 8
Data/hora: 05/06/2024 12:32:49

Detection of antibodies to Toxoplasma gondii in wild animals in Brazil
Provedor de dados: 80
Autores: Silva,Rodrigo Costa da; Machado,Gustavo Puglia; Cruvinel,Tatiane Morosini de Andrade; Cruvinel,Ciro Alexandre; Langoni,Helio.
Background Toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite,Toxoplasma gondii, that affects all warm-blooded animals, including wild animals. The increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. In this study, the occurrence of T. gondii infection was investigated by the modified agglutination test (MAT) in 26 different species of run over and injured wild animals that were treated at a Brazilian university veterinary hospital, from June 2007 to August 2008.Findings Of the studied animals, six (23.1%; CI95% 11.1-42.2%) had T. gondii antibodies, with titers equal to 10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Natural infection; Antibodies; Wild animals; MAT.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200603

Leptospiral antibodies in wild boars (Sus scrofa) bred in Brazil
Provedor de dados: 80
Autores: Fornazari,F; Camossi,LG; Silva,RC; Guazzelli,A; Ribeiro,MG; Chiacchio,SB; Langoni,H.
In the recent years, the wild boar (Sus scrofa) trade has increased in Brazil. The present study aimed to detect Leptospira spp. infection in 308 blood samples from wild boars bred in São Paulo state, Brazil. The microscopic agglutination test (MAT) was performed using 29 serovars. Sixty three (63; 20.45%) animals tested positive against the serovars Hardjo (29/63; 46.0%), Copenhageni (11/63; 17.4%), Pomona (8/63; 12.7%), Pyrogenes (4/63; 6.3%), Wolfii (4/63; 6.3%), Autumnalis (3/63; 4.7%), Icterohaemorraghiae (2/63; 3.1%), and Hardjo-miniswajezak (1/63; 15.8%). These results indicate that captive wild boars were infected with Leptospira spp. and may represent a source of infection to humans and other animals.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wild boar; Sus scrofa; Leptospira spp.; MAT; Zoonosis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100012

Evidenciação sorológica de exposição à Leptospira sp em mamíferos silvestres
Provedor de dados: 195
Autores: Rodrigues, Taizi; Oliveira, Fernanda Santana; Oliveira, Vinícius; Curvelo, Vitor; Oriá, Arianne Pontes; Pinna, Melissa Hanzen.
Foi efetuado um inquérito sorológico da leptospirose em diferentes espécies de mamíferos silvestres, mantidos em cativeiro. Foram colhidas amostras de 88 animais, dos quais 40 primatas, 24 felídeos, 15 cervídeos, oito canídeos e um animal da Família Ursidea, totalizando 18 espécies. Os animais foram contidos quimicamente com a associação de fármacos sedativos, e posteriormente foram submetidos aos exames físicos e coleta de sangue. Após a coleta, as amostras foram resfriadas e transportadas até o laboratório para centrifugação, sendo o soro estocado. O diagnóstico sorológico para leptospirose foi realizado de acordo com a recomendação técnica da OMS, por meio da soroaglutinação microscópica (MAT) com antígenos vivos, com cada amostra sorológica testada...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirose; MAT; Animais silvestres.
Ano: 2013
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/21409

Comparación entre un ELISA indirecto y la técnica de aglutinación microscópica para la detección de anticuerpos antileptospirales en caninos
Provedor de dados: 101
Autores: García,Radamés L.; Feraud,Dania; Lugo,Sonia; Machado,Héctor; Abeledo,María A.
Con el objetivo de comparar un ELISA indirecto elaborado a partir de Leptospira biflexa serogrupo Semaranga serovar Patoc, con la técnica de aglutinación microscópica (de su sigla en inglés MAT) en el diagnóstico serológico de la leptospirosis canina, se utilizaron 77 muestras de suero sanguíneo de caninos con sospecha de leptospirosis (Grupo 1) y 84 muestras de animales aparentemente sanos (Grupo 2), los cuales fueron investigados simultáneamente por el ensayo inmunoenzimático y por la técnica de aglutinación microscópica (MAT). Los valores de prevalencia obtenidos por el MAT y el ELISA, así como entre ambos grupos no mostraron diferencias significativas, lo que indica la coincidencia entre ambos métodos y la existencia de una amplia circulación de cepas...
Tipo: Journal article
Palavras-chave: Leptospirosis canina; Diagnóstico serológico; ELISA; MAT.
Ano: 2014
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200007

Interference of vaccinal antibodies on serological diagnosis of leptospirosis in vaccinated buffalo using two types of commercial vaccines
Provedor de dados: 58
Autores: Nardi Júnior,Geraldo de; Genovez,Margareth Elide; Ribeiro,Marcio Garcia; Castro,Vanessa; Jorge,André Mendes.
The vaccinal antibodies interference represents one of the Microscopic Agglutination test - MAT limitation in the animal leptospirosis serum diagnosis. Prospective studies showing the dimensions of this effect are rare in buffaloes. This study aimed to determine the anti-Leptospira serum agglutinin profile in vaccinated female buffaloes using two types of commercial vaccines against leptospirosis: bacterin (whole bacterial cell) and purified outer membrane and to evaluate the vaccinal interference on serum diagnosis. Three groups of 11 adult buffalo females were established: G1-control, non-vaccinated, G2- vaccinated with bacterin vaccine with six serovars, G3- outer membrane purified vaccine with five serovars. A booster dose was administrated 30 days...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Buffalo leptospirosis; Vaccination; Serum diagnosis; MAT.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200033

Human leptospirosis: occurrence of serovars of Leptospira spp. in the state of Minas Gerais, Brazil, from 2008 to 2012
Provedor de dados: 58
Autores: Oliveira,Marluce Aparecida Assunção; Leal,Élida Aparecida; Correia,Max Assunção; Serufo Filho,José Carlos; Dias,Ricardo Souza; Serufo,José Carlos.
Abstract  Background: Leptospirosis is an infectious and acute disease caused by Leptospira spp. that have high epidemic potential. This study verified the main Leptospira spp. serovars detected by MAT from serum of patients with suspicion of leptospirosis from 2008 to 2012 in Minas Gerais State.  Methods: The laboratory received sera from 4654 patients. All serum were screened by IgM-ELISA according to the manufacturer's instructions. Each sample reactive or indeterminate were tested against twenty-four serovars of Leptospira by MAT.  Results: In this study, 597 patients were classified as reactive on MAT. Only 301 patients were confirmed by laboratory test. It was not possible confirmation by laboratory diagnosis of 296 patients. Among the samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Serovar; Seroprevalence; Epidemiology; MAT.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300483

Leptospirosis serosurvey in bovines from Brazilian Pantanal using IGG ELISA with recombinant protein LipL32 and microscopic agglutination test
Provedor de dados: 58
Autores: Tomich,Renata Graça Pinto; Bomfim,Maria Rosa Quaresma; Koury,Matilde Cota; Pellegrin,Aiesca Oliveira; Pellegrin,Luiz Alberto; Ko,Albert Icksang; Barbosa-Stancioli,Edel Figueiredo.
This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine Leptospirosis; IgG ELISA rLipL32; MAT.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400017

Seroprevalence of Leptospira spp. infection in sheep from northwest of Rio Grande do Sul, Brazil
Provedor de dados: 65
Autores: Consalter,Angélica; Zanella,Eraldo Lourenso; Martins,Gabriel Mendes de Souza; Araujo,Hugo Libonati de; Lilenbaum,Walter; Souza,Guilherme Nunes de; Ferreira,Ana Maria Reis.
ABSTRACT: Leptospirosis is a zoonosis that is widely distributed in tropical countries. This infection is also associated with reproductive losses in livestock, which has a significant economic impact. The objective of this study is to investigate the seroprevalence and risk factors associated with leptospiral infection in commercial sheep flocks from the northwestern mesoregion of Rio Grande do Sul. Serum samples were analyzed in 319 sheep using the Microscopic Agglutination Test (MAT). At the time of sampling, an individual and herd questionnaire was carried out to investigate the risk factors. On an individual level, positive reactions were observed in 5.6% (18/319) of the studied sera, with Sejroe being the most prevalent serogroup. Animals &gt;3 years...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira spp.; Sheep; MAT; Seroprevalence.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200451