Consulta: subjectFacets:"Macrophage"
Registros recuperados: 23
Data/hora: 05/06/2024 02:34:14

Inhibition of NF-kB 1 (NF-kBp50) by RNA interference in chicken macrophage HD11 cell line challenged with Salmonella enteritidis
Provedor de dados: 74
Autores: Chiang,Hsin-I; Berghman,Luc R.; Zhou,Huaijun.
The NF-kB pathway plays an important role in regulating the immunity response in animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit NF-kB 1 expression and to elucidate the role of NF-kB in the signal transduction pathway of the Salmonella challenge in the chicken HD11 cell line. The cells were transfected with either NF-kB 1 siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or the negative control siRNA for 24 h, followed by Salmonella enteritidis (SE) challenge or non-challenge for 1 h and 4 h. Eight candidate genes related to the signal pathway of SE challenge were selected to examine the effect of NF-kB 1 inhibition on their expressions by mRNA quantification. The results showed that, with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNA interference; NF-kBp50; Macrophage; Salmonella; Chicken.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300013

Caractérisation morphologique et fonctionnelle des hémocytes d'Ostrea edulis et de Crassostrea gigas, mollusques bivalves. Etude in vitro de leurs interactions avec le protozoaire Bonamia ostreae (Ascetospora)
Provedor de dados: 5
Autores: Jean Chagot, Dominique.
La morphologie et l'équipement enzymatique des hémocytes de deux espèces d'huîtres, Ostea edulis et Crassostrea gigas ont été étudiés. L'analyse des fonctions des hémocytes dans les mécanismes immunitaires a permis de mettre en évidence leur rôle de macrophage, mais n'a pas fait apparaître de différence interspécifique dans les phénomènes des cytocidie oxygène dépendants, susceptible d'expliquer la permissivité d'Ostrea edulis pour Bonamia ostreae et la résistance de Crassostrea gigas. L'infection in vitro de primocultures d'hémocytes des deux espèces démontre un mécanisme d'entrée par phagocytose, doublé chez Ostrea edulis d'une possible pénétration active. L'analyse des récepteurs impliqués dans la phagocytose (récepteurs pour des opsonines plasmatiques,...
Tipo: Text
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Hémocyte; Morphologie; Macrophage; Caractérisation enzymatiques; Chimioluminescence; Interaction in vitro; Phagocytose; Récepteur.
Ano: 1989
URL: http://archimer.ifremer.fr/doc/00210/32102/30559.pdf

Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages
Provedor de dados: 19
Autores: NISHIKAWA, Yoshifumi; IBRAHIM, Hany M.; KAMEYAMA, Kyohko; SHIGA, Ikumi; HIASA, Jun; XUAN, Xuenan; 西川, 義文; 玄, 学南.
The intracellular protozoan Toxoplasma gondii lacks the ability to synthesize sterol and scavenges cholesterol from the lowdensity lipoprotein receptor (LDLR) pathway of its host to facilitate replication. Sterol biosynthesis inhibitors, however, have a demonstrated anti-Toxoplasma effect. In this study, we examined the host mevalonate pathway as a novel source of cholesterol for T. gondii and its effects on parasite growth in macrophages. Parasite growth did not significantly change in the absence of LDLR or when LDL was exogenously supplemented. Lovastatin and compactin, both inhibitors of hydroxymethylglutaryl-CoA (HMG-CoA) reductase in the mevalonate pathway, significantly inhibited T. gondii growth in both wild-type and LDLR-knockout macrophages....
Palavras-chave: Cholesterol; Lipid metabolism; Macrophage; Parasitology; Toxoplasma gondii.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3583

トキソブラズマ溶解抗原(TLA)によって誘導される細胞障害性における脾臓の付着性細胞の役割
Provedor de dados: 19
Autores: MIYAHARA, Kazuro; TOSE, Shinichi; SAKURAI, Haruhisa; IGARASHI, Ikuo; SAITO, Atsushi; HIROSE, Tsuneo; SUZUKI, Naoyoshi; 宮原, 和郎; 五十嵐, 郁男.
In order to identify mechanisms responsible for the anti-tumor effects of Toxoplasma lysate antigen (TLA), we used an in vitro 51Cr release assay to study the functional properties of plastic-adherent cells during induction of splenic cytotoxic activity by TLA. Cytotoxic activity of non-adherent cells was measured in all experiments after a 6 days incubation. Induction of cytotoxic non-adherent cells by TLA required the presence of plastic-adherent spleen cells. In contrast, rhIL-2 alone was able to induce transformation of cytotoxic non-adherent cells from non-adherent spleen cells. Contact between adherent and non-adherent spleen cells was necessary for successful induction of cytotoxic non-adherent cells by TLA. Treatment of spleen cells with...
Palavras-chave: Adherent cell; Cytotoxic activity; Macrophage; Spleen cell; Toxoplasma lysate antigen.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4065

Species-specific Interaction of Leishmania Promastigotes with Macrophages : Effect of Previous Incubation of the Host Cells with Enzymes, Monosaccharides and  Lectin
Provedor de dados: 19
Autores: Ghosh, Dilip Kumar.
Palavras-chave: Leishmaniases; Leishmania; Macrophage; Ligands; Receptors.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/206

The effect of arginine dietary supplementation in broiler breeder hens on offspring humoral and cell-mediated immune responses
Provedor de dados: 89
Autores: Murakami,AE; Silva,LMS da; Fernandes,JIM; Silveira,TGV; Garcez Neto,AF.
The influence of supplementing the diet of broiler breeder hens with arginine (Arg) on their offspring's humoral and cell-mediated immune response was evaluated in two experiments. In experiments I and II, breeder hens were fed diets containing graded levels of Arg (0.943, 1.093, 1.243, 1.393 and 1.543% digestible Arg). In experiment I, the offspring was randomly grouped according to the treatment received by the breeder hens, with five levels of Arg in the maternal diet and six replicates, giving a total 30 experimental units. In experiment II, the offspring were grouped in accordance with the treatment received by the breeder hens; however, Arg was added to the starter diet (1.300, 1.450, 1.600, 1.750 and 1.900% digestible Arg) and also the growing diet...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine; Macrophage; Maternal diet; Offspring immunity.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200009

Inhibitory effects on the production of inflammatory mediators and reactive oxygen species by Mori folium in lipopolysaccharide-stimulated macrophages and zebrafish
Provedor de dados: 42
Autores: KWON,DA HYE; JEONG,JIN WOO; CHOI,EUN OK; LEE,HYE WON; LEE,KI WON; KIM,KI YOUNG; KIM,SUNG GOO; HONG,SU HYUN; KIM,GI-YOUNG; PARK,CHEOL; HWANG,HYE-JIN; SON,CHANG-GUE; CHOI,YUNG HYUN.
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mori folium; Inflammation; ROS; Macrophage; Zebrafish.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661

Immunology and immunopathology of African trypanosomiasis
Provedor de dados: 42
Autores: Vincendeau,Philippe; Bouteille,Bernard.
Major modifications of immune system have been observed in African trypanosomiasis. These immune reactions do not lead to protection and are also involved in immunopathology disorders. The major surface component (variable surface glycoprotein,VSG) is associated with escape to immune reactions, cytokine network dysfunctions and autoantibody production. Most of our knowledge result from experimental trypanosomiasis. Innate resistance elements have been characterised. In infected mice, VSG preferentially stimulates a Th 1-cell subset. A response of &lt;FONT FACE=Symbol&gt;gd&lt;/FONT&gt; and CD8 T cells to trypanosome antigens was observed in trypanotolerant cattle. An increase in CD5 B cells, responsible for most serum IgM and production of autoantibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosome; African trypanosomiasis; Immunology; Macrophage; Lymphocytes; Nitric oxide; Cytokine; Autoantibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400004

Simvastatin acts as an inhibitor of interferon gamma-induced cycloxygenase-2 expression in human THP-1 cells, but not in murine RAW264.7 cells
Provedor de dados: 47
Autores: Lee,Chang Seok; Shin,Yong Jae; Won,Cheolhee; Lee,Yun-Song; Park,Chung-Gyu; Ye,Sang-Kyu; Chung,Myung-Hee.
Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNgamma)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNgamma-induced COX-2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNgamma-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNgamma-induced COX-2 expression in murine RAW264.7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte; Macrophage; INFgamma; STAT1/3; SOCS1/3.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200005

Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
Provedor de dados: 48
Autores: LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A.
Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine...
Tipo: Journal article
Palavras-chave: Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004

Preventive effect of Ligularia fischeri on inhibition of nitric oxide in lipopolysaccharide-stimulated RAW 264.7 macrophages depending on cooking method
Provedor de dados: 48
Autores: Park,Hee-Sook; Choi,Hye-Young; Kim,Gun-Hee.
BACKGROUND: Ligularia fischeri (common name Gomchwi) is known for its pharmaceutical properties and used in the treatment of jaundice, scarlet-fever, rheumatoidal arthritis, and hepatic diseases; however, little is known about its anti-inflammatory effect. In this study the influence of blanching and pan-frying on the anti-inflammatory activity of Ligularia fischeri (LF) was evaluated. RESULTS: Fresh LF and cooked LF showed no significant effect on the viability of macrophages after 24 h incubation. Fresh LF was found to be the most potent inhibitor of nitric oxide (NO) production at 100 &amp;#956;g/ml, while pan-fried LF showed little inhibitory effect on lipoloysaccharide (LPS) stimulated murine machrophage RAW264.7 cells. In contrast with its effect on...
Tipo: Journal article
Palavras-chave: Chlorogenic acid; Inflammation; Ligularia fischeri; Macrophage.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100063

Salmonella enterica serovar Typhi plasmid pR ST98 enhances intracellular bacterial growth and S. typhi-induced macrophage cell death by suppressing autophagy
Provedor de dados: 55
Autores: He,Peiyan; Wu,Shuyan; Chu,Yuanyuan; Yang,Yanru; Li,Yuanyuan; Huang,Rui.
OBJECTIVES: Plasmid pR ST98 is a hybrid resistance-virulence plasmid isolated from Salmonella enterica serovar Typhi (S. typhi). Previous studies demonstrated that pR ST98 could enhance the virulence of its host bacteria. However, the mechanism of pR ST98-increased bacterial virulence is still not fully elucidated. This study was designed to gain further insight into the roles of pR ST98 in host responses. METHODS: Human-derived macrophage-like cell line THP-1 was infected with wild-type (ST8), pR ST98-deletion (ST8-ΔpR ST98), and complemented (ST8-c-pR ST98) S. typhi strains. Macrophage autophagy was performed by extracting the membrane-unbound LC3-I protein from cells, followed by flow cytometric detection of the membrane-associated fraction of LC3-II....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plasmid pR ST98; Macrophage; Autophagy; Intracellular bacterial growth; Cell death.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300008

Thalidomide and pentoxifylline block the renal effects of supernatants of macrophages activated with Crotalus durissus cascavella venom
Provedor de dados: 56
Autores: Martins,A.M.C.; Nobre,A.C.L.; Almeida,A.C.; Bezerra,G.; Lima,A.A.M.; Fonteles,M.C.; Monteiro,H.S.A.
Because thalidomide and pentoxifylline inhibit the synthesis and release of tumor necrosis factor-alpha (TNF-alpha), we determined the effect of these drugs on the renal damage induced by supernatants of macrophages activated with Crotalus durissus cascavella venom in order to identify the role of TNF-alpha in the process. Rat peritoneal macrophages were collected with RPMI medium and stimulated in vitro with C.d. cascavella venom (10 µg/ml) in the absence and presence of thalidomide (15 µM) or pentoxifylline (500 µM) for 1 h and washed and kept in culture for 2 h. Supernatant (1 ml) was tested on an isolated perfused rat kidney (N = 6 for each group). The first 30 min of each experiment were used as control. The supernatant was added to the perfusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nephrotoxicity; Crotalus durissus cascavella; Pentoxifylline; Thalidomide; Macrophage.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000012

Extracellular ATP in the lymphohematopoietic system: P2Z purinoceptors and membrane permeabilization
Provedor de dados: 56
Autores: Persechini,P.M.; Bisaggio,R.C.; Alves-Neto,J.L.; Coutinho-Silva,R.
The effects of extracellular nucleosides and nucleotides on many organs and systems have been recognized for almost 50 years. The effects of extracellular ATP (ATPo), UTPo, ADPo, and other agonists are mediated by P2 purinoceptors. One of the most dramatic effects of ATPo is the permeabilization of plasma membranes to low molecular mass solutes of up to 900 Da. This effect is evident in several cells of the lymphohematopoietic system and is supposed to be mediated by P2Z, an ATP4--activated purinoceptor. Here, we review some basic information concerning P2 purinoceptors and focus our attention on P2Z-associated phenomena displayed by macrophages. Using fluorescent dye uptake, measurement of free intracellular Ca2+ concentration and electrophysiological...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATP; P2Z purinoceptor; Macrophage; Monocyte; T cell; Permeabilization.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100004

Phagocytosis by macrophages mediated by receptors for denatured proteins - dependence on tyrosine protein kinases
Provedor de dados: 56
Autores: Hespanhol,M.R.; Mantovani,B.
Previous studies have demonstrated that some components of the leukocyte cell membrane, CR3 (Mac-1, CD11b/CD18) and p150/95, are able to bind to denatured proteins. Thus, it is of interest to know which effector functions of these cells can be triggered by these receptors when they interact with particles or surfaces covered with denatured proteins. In the present study we analyzed their possible role as mediators of phagocytosis of red cells covered with denatured bovine serum albumin (BSA) by mouse peritoneal macrophages. We observed that a) macrophages are able to recognize (bind to) these red cells, b) this interaction can be inhibited by denatured BSA in the fluid phase, c) there is no phagocytosis of these particles by normal macrophages, d)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phagocytosis; Denatured proteins; Macrophage; SS2 Integrins; Tyrosine protein kinases.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300015

Outward potassium current oscillations in macrophage polykaryons: extracellular calcium entry and calcium-induced calcium release
Provedor de dados: 56
Autores: Saraiva,R.M.; Masuda,M.O.; Oliveira-Castro,G.M.
Outward current oscillations associated with transient membrane hyperpolarizations were induced in murine macrophage polykaryons by membrane depolarization in the absence of external Na+. Oscillations corresponded to a cyclic activation of Ca2+-dependent K+ currents (IKCa) probably correlated with variations in intracellular Ca2+ concentration. Addition of external Na+ (8 mM) immediately abolished the outward current oscillations, suggesting that the absence of the cation is necessary not only for their induction but also for their maintenance. Oscillations were completely blocked by nisoldipine. Ruthenium red and ryanodine reduced the number of outward current cycles in each episode, whereas quercetin prolonged the hyperpolarization 2- to 15-fold. Neither...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrophage; Macrophage polykaryon; Ca2+-dependent K+ current; Membrane potential oscillations; Calcium-induced calcium release; Ca2+ ATPase.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100015

Role of nitric oxide and superoxide in Giardia lamblia killing
Provedor de dados: 56
Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (&amp;gt;90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015

Immunoregulatory and anti-inflammatory effects of n-3 polyunsaturated fatty acids
Provedor de dados: 56
Autores: Calder,P.C.
1. Fish oils are rich in the long-chain n-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic (20:5n-3) and docosahexaenoic (22:6n-3) acids. Linseed oil and green plant tissues are rich in the precursor fatty acid, &lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;-linolenic acid (18:3n-3). Most vegetable oils are rich in the n-6 PUFA linoleic acid (18:2n-6), the precursor of arachidonic acid (20:4n-6). 2. Arachidonic acid-derived eicosanoids such as prostaglandin E2 are pro-inflammatory and regulate the functions of cells of the immune system. Consumption of fish oils leads to replacement of arachidonic acid in cell membranes by eicosapentaenoic acid. This changes the amount and alters the balance of eicosanoids produced. 3. Consumption of fish oils diminishes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lymphocyte; Macrophage; Immune system; Inflammation; Fish oil; Polyunsaturated fatty acid; Cytokine; Eicosanoid.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400002

Influence of acute renal failure on the mononuclear phagocytic system
Provedor de dados: 56
Autores: Sousa,V.R.A.; Sousa,A.A.; Petroianu,A.; Simal,C.J.R.; Barbosa,A.J.A.
Several studies show the ability of macrophages to remove particles injected into the bloodstream. This function seems to be increased in the presence of acute renal failure. The objective of the present study was to assess the phagocytic function of the main organs (spleen, liver and lung) of the mononuclear phagocytic system in renal and postrenal failures. Fifteen rats (250-350 g) were divided into three groups (N = 5): group I - control; group II - ligature of both ureters, and group III - bilateral nephrectomy. On the third postoperative day, all animals received an iv injection of 1 ml/kg 99mTc sulfur colloid. Blood samples were collected for the assessment of plasma urea, creatinine, sodium, and potassium concentrations and arterial gasometry....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renal failure; Reticuloendothelial system; Phagocytosis; Macrophage; Scintigraphy.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900010

Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide
Provedor de dados: 56
Autores: Ramos,M.G.; Rabelo,F.L.A.; Duarte,T.; Gazzinelli,R.T.; Alvarez-Leite,J.I.
We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose- and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The levels of butyrate-induced apoptosis in macrophages were enhanced by co-treatment with 1 µg/ml bacterial lipopolysaccharide (LPS). However, our data indicate that apoptosis induced by butyrate and LPS involves different mechanisms. Thus, LPS-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrate; Apoptosis; Caspase; Nitric oxide; Cytokine; Macrophage.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004

Galectin-1, an alternative signal for T cell death, is increased in activated macrophages
Provedor de dados: 56
Autores: Rabinovich,G.A.; Riera,C.M.; Sotomayor,C.E.
Galectin-1 belongs to an evolutionarily conserved family of animal ß-galactoside-binding proteins, which exert their functions by crosslinking the oligosaccharides of specific glycoconjugate ligands. During the past decade, attempts to identify the functional role of galectin-1 suggested participation in the regulation of the immune response. Only in the last few years has the molecular mechanism involved in these properties been clearly elucidated, revealing a critical role for galectin-1 as an alternative signal in the generation of T cell death. In the present study we will discuss the latest advances in galectin research in the context of the regulation of the immune response, not only at the central level but also at the periphery. Moreover, we will...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Galectin-1; Apoptosis; Immunomodulation; Macrophage.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500009

Effect of cyhalothrin on Ehrlich tumor growth and macrophage activity in mice
Provedor de dados: 56
Autores: Quinteiro-Filho,W.M.; Righi,D.A.; Palermo-Neto,J.
Cyhalothrin, a pyrethroid insecticide, induces stress-like symptoms, increases c-fos immunoreactivity in the paraventricular nucleus of the hypothalamus, and decreases innate immune responses in laboratory animals. Macrophages are key elements in cellular immune responses and operate at the tumor-host interface. This study investigated the relationship among cyhalothrin effects on Ehrlich tumor growth, serum corticosterone levels and peritoneal macrophage activity in mice. Three experiments were done with 10 experimental (single gavage administration of 3.0 mg/kg cyhalothrin daily for 7 days) and 10 control (single gavage administration of 1.0 mL/kg vehicle of cyhalothrin preparation daily for 7 days) isogenic BALB/c mice in each experiment. Cyhalothrin i)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyhalothrin; Corticosterone; Macrophage; Ehrlich tumor; Innate immunity; HPA axis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000006

Cissampelos sympodialis Eichl (Menispermaceae) leaf extract induces interleukin-10-dependent inhibition of Trypanosoma cruzi killing by macrophages
Provedor de dados: 56
Autores: Alexandre-Moreira,M.S.; Freire-de-Lima,C.G.; Trindade,M.N.; Castro-Faria-Neto,H.C.; Piuvezam,M.R.; Peçanha,L.M.T.
The aqueous fraction of the ethanolic extract (AFL) of Cissampelos sympodialis Eichl (Menispermaceae), popularly known as milona, has been shown to have both immunosuppressive and anti-inflammatory effects. In the present study we investigated the modulation of macrophage antimicrobicidal activity by in vitro treatment with the extract from C. sympodialis. Normal and thioglycolate-elicited mouse peritoneal macrophages were infected in vitro with the protozoan Trypanosoma cruzi DM28c clone. We observed that the AFL (used at doses ranging from 13 to 100 µg/ml) increased T. cruzi growth and induced a 75% reduction in nitric oxide production. This inhibition could be mediated by the stimulation of macrophage interleukin-10 (IL-10) secretion since the in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cissampelos sympodialis; Macrophage; Trypanosoma cruzi; Nitric oxide; Interleukin-10.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200006