Consulta: subjectFacets:"Marker-assisted selection"
Registros recuperados: 43
Data/hora: 18/06/2024 06:25:28

Genetic characterization of coat color genes in Brazilian Crioula sheep from a conservation nucleus
Provedor de dados: 86
Autores: Cavalcanti,Lilian Cristina Gomes; Moraes,José Carlos Ferrugem; Faria,Danielle Assis de; McManus,Concepta Margaret; Nepomuceno,Alcebiades Renato; Souza,Carlos José Hoff de; Caetano,Alexandre Rodrigues; Paiva,Samuel Rezende.
Abstract: The objective of this work was to identify single nucleotide polymorphisms (SNPs) in resequencing data from MC1R, ASIP, and TYRP1 genes derived from Crioula sheep (Ovis aris) with different coat colors. Polymorphisms in the ASIP (agouti-signaling protein), MC1R (melanocortin 1 receptor), and TRYP1 (tyrosinase-related protein 1) genes were analyzed in 115 sheep from Embrapa’s conservation nucleus of crioula sheep, in Brazil. A total of 7,914 bp were sequenced per animal, and 14 SNPs were identified. Two additional assays were performed to detect duplications and deletions in the ASIP gene. Ninety-five percent of the coat color variation was explained by epistatic interactions observed between specific alleles in the MC1R and ASIP genes. Evidence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ovis aris; ASIP; Genetic resources conservation; Marker-assisted selection; MC1R; TYRP1.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000800615

QTLs for resistance to soybean cyst nematode, races 3, 9, and 14 in cultivar Hartwig
Provedor de dados: 86
Autores: Ferreira,Marcia Flores da Silva; Cervigni,Gerardo Domingo Lucio; Ferreira,Adésio; Schuster,Ivan; Santana,Fernanda Abreu; Pereira,Waldir Dias; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Breeding; Marker-assisted selection; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400012

Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle
Provedor de dados: 96
Autores: Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira.
The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C&gt; T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C&gt;T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004

Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle
Provedor de dados: 96
Autores: Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida; Siqueira,Fabiane.
ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A&gt;G (CAST/DdeI) and AF248054.2:g.6545C&gt;T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal breeding; Marker-assisted selection; PCR-RFLP; SNP; Tenderness.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100311

Identification of the Er1 resistence gene and RNase S-alleles in Malus prunifolia var. ringo rootstock
Provedor de dados: 63
Autores: Agapito-Tenfen,Sarah Zanon; Dantas,Adriana Cibele de Mesquita; Denardi,Frederico; Nodari,Rubens Onofre.
Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) is a major insect pest that has significant economic impact on apple growers worldwide. Modern breeding technologies rely on several molecular tools to help breeders select genetic determinants for traits of interest. Consequently, there is a need for specific markers linked to the genes of interest. Apple scions and rootstocks have an additional barrier to the introduction of pest resistance genes due to the presence of self-incompatibility S-RNase alleles. The genetic characterization and early identification of these alleles can amplify the contribution of a breeding program to the selection of resistant genitors that are as compatible as possible. In this study, we identified the Er1 gene involved in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus pumila; Woolly apple aphid; Self-incompatibility; Bulk segregant analysis; Marker-assisted selection.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100062

Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance
Provedor de dados: 63
Autores: Fritsche-Neto,Roberto; Souza,Thiago Lívio Pessoa Oliveira de; Pereira,Helton Santos; Faria,Luís Cláudio de; Melo,Leonardo Cunha; Novaes,Evandro; Brum,Itaraju Junior Baracuhy; Jannink,Jean-Luc.
ABSTRACT: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colletotrichum lindemuthianum; Pseudocercospora griseola; Disease resistance; Elite lines; Marker-assisted selection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001400321

การพัฒนาสายพันธุ์ข้าวต้านทานโรคขอบใบแห้งโดยวิธีการผสมกลับและคัดเลือก ด้วยเครื่องหมายโมเลกุล
Provedor de dados: 106
Autores: Kanokon Yaodam; Prapa Sripichitt; Tanee Sriwongchai; Supaporn Junbuathong.
Bacterial leaf blight (BLB) disease is caused by bacteria Xanthomonas oryzae pv. oryzae (Xoo). Infection of this disease can occur at all stages of rice plants from seedling to heading. It can cause as high as 80 % yield reduction. The utilization of resistant varieties have been considered to be the most effective way to control the disease. At present, rice varieties are not widely resistant to BLB disease which has many strains. The resistant varieties having different BLB resistant genes are associated with the different strains of the pathogen. It is necessary to improve rice varieties for widely resistance to BLB disease. Rice improvement for BLB disease resistance was conducted by crossing between a line (RGDU07097-1-MAS-8-9-B-1) with BLB disease...
Tipo: Collection
Palavras-chave: Rice; Bacterial leaf blight disease; Xanthomonas oryzae pv. oryzae; Backcrossing; Marker-assisted selection; ข้าว; โรคขอบใบแห้ง; Xanthomonas oryzae pv. oryzae; การผสมกลับ; การคัดเลือกโดยเครื่องหมายโมเลกุล.
Ano: 2015
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5929

การพัฒนาข้าวพันธุ์บางแตนให้ต้านทานโรคไหม้แบบผสมกลับ โดยใช้เครื่องหมายโมเลกุล
Provedor de dados: 106
Autores: Reunreudee Kaewcheenchai; Sommai Sriwisut; Apichart Noenplab; Acharaporn Na Lampang Noenplab; Kittima Ruksopa; Peera Dungsoongnern; Udompan Promnart; Nila Rasidee.
Blast disease is a major rice disease causing significant rice yield loss worldwide. These are several methods to control this disease, but the most effective and most suitable method is to use blast resistant varieties. Prachin Buri Rice Research Center has released a rice variety ‘Bahng Taen’, which is resistant to blast in the Eastern region. Bahng Taen is a non-glutinous, photoperiod insensitive, early maturity variety with high yielding ability. However, the ability to resist to blast of this variety is declining drastically. It is, therefore, necessary to improve blast resistance in Bahng Taen by introgressing blast resistant genes into its background from two donors; PSL’07F6No.2 and PSL’07F6No.5. These two resistant lines were derived from the...
Tipo: Collection
Palavras-chave: Rice; Blast disease; Backcrossing; Marker-assisted selection; Bahng Taen; ข้าว; โรคไหม้; การผสมกลับ; การคัดเลือกโดยใช้เครื่องหมายโมเลกุล; บางแตน.
Ano: 2015
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5943

การปรับปรุงข้าวโพดสายพันธุ์แท้ให้ต้านทานโรคราน้ำค้างโดยวิธี Marker-assisted Selection
Provedor de dados: 106
Autores: Chaba Jampatong; Sansern Jampatong; Wanchai Yenpetch; Sanon Suksatan; Anucha Mekarun; Athiwat Songpinich.
Downy mildew (DM), Peronosclerospora sorghi (Weston and Uppal) C.G. Shaw, is one of the most destructive diseases of maize (Zea mays L.) in Thailand.  Understanding the genetic basis of downy mildew resistance could increase the efficiency of breeding for disease resistant germplasm. The objectives of this study were to use the SSR primers associated with QTLs which produced major effects, umc1072 found in chromosome bin 5.07, and bnlg1867 found in chromosome bin 6.01 used to transfer resistance alleles to susceptible lines by marker-assisted selection. A backcross method was carried out in this research. Agron18 and Nei452006 were susceptible lines used as recurrent parent, Nei9008 was resistant line used as donor parent. The results indicated that...
Palavras-chave: Maize; Downy mildew disease; SSR primers; Marker-assisted selection; ข้าวโพด; โรคราน้ำค้าง; การปรับปรุงพันธุ์; พันธุ์แท้; ความต้านทานโรค.
Ano: 2010
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4661

Herança genética e associação de marcadores microssatélites à resistência à liriomyza sativae em meloeiro.
Provedor de dados: 119
Autores: LEITÃO, L. R. G.
O meloeiro (Cucumis melo L.) é uma hortaliça com destaque mundial. No Brasil, tem relevante importância socioeconômica na região Nordeste. A mosca-minadora, Liriomyza spp. (Diptera: Agromyzidae), é a principal praga da cultura, ocasionando sérios danos à planta e, consequentemente, reduzindo a produção e a qualidade dos frutos. O uso de genótipos resistentes constitui o método mais eficiente e sustentável de controle, entretanto, poucas fontes de resistência a esse inseto já foram identificadas em germoplasma de meloeiro. A elucidação da herança genética e o uso de marcadores moleculares são ferramentas que auxiliam na introgressão da resistência à mosca-minadora em híbridos comerciais de meloeiro. Considerando duas fontes de resistência à mosca-minadora...
Tipo: Teses
Palavras-chave: Cucumis Melo; Leafminers; Antibiosis; USSR; Marker-assisted selection.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102482

Assisted-selection of naturally caffeine-free coffee cultivars-characterization of SNPs from a methyltransferase gene.
Provedor de dados: 119
Autores: FAVORETTO, P.; SILVA, C. C. da; TAVARES, A. G.; GIATTI, G.; MORAES, P. F.; LOBATO, M. T. V.; SILVAROLLA, M. B.; OLIVEIRO FILHO, G.; MALUF, M. P.
Breeding of caffeine-free coffee cultivars require tools for an early selection of progenies bearing this later trait. Genes from caffeine synthesis and degradation represent major targets for the development of molecular markers for assisted selection. In this study, we characterized SNPs identified on the caffeine synthase gene from AC1 mutant, a naturally caffeine-free arabica coffee plant. Molecular analysis of normal and mutant sequences indicates the occurrence of SNPs in protein domains, potentially associated with caffeine synthesis in coffee. Progenies F2, F1BC1 and BC from crosses of AC mutants and elite cultivars were evaluated regarding caffeine content in grains and genomic segregation profile of selected SNPs. Genotyping analysis allowed the...
Tipo: Artigo de periódico
Palavras-chave: Caffeine content; Caffeine synthase; Coffee; Molecular markers; Cafeína; Marcador Molecular.; Marker-assisted selection.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113244

Seleção genômica ampla (GWS) e associação genômica ampla (GWAS) no melhoramento de forrageiras: abordagem conceitual, genética quantitativa e aplicações.
Provedor de dados: 119
Autores: MIQUELONI, D. P.; RESENDE, R. M. S.; ASSIS, G. M. L. de.
Atualmente, desenvolvimento da tecnologia de análise genômica de alta resolução e alto rendimento tem a capacidade de gerar informações sobre milhões de pares de bases em um único ensaio, permitindo a detecção de variação em um único nucleotídeo de uma sequência de DNA. Este contexto possibilitou o surgimento de novas abordagens para a seleção assistida por marcadores, dentre elas os estudos de associação genômica ampla (GWAS), que visam o mapeamento genético por meio das associações entre os locos e a característica fenotípica na população e buscam detectar efeitos dos genes sobre os valores genéticos dos indivíduos; e a seleção genômica ampla (GWS), que detecta genótipos favoráveis por meio de informações genotípicas para inferir sobre os valores...
Tipo: Artigo de periódico
Palavras-chave: Associação genômica ampla (GWAS); Seleção genômica ampla (GWS); Fitomejoramiento; Selección asistida por marcadores; Melhoramento Genético Vegetal; Seleção Genética; Marcador Genético; Plant breeding; Genetic markers; Marker-assisted selection.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118273

Genome prediction accuracy of common bean via Bayesian models.
Provedor de dados: 119
Autores: BARILI, L. D.; VALE, N. M. do; SILVA, F. R. e; CARNEIRO, J. E. de S.; OLIVEIRA, H. R. de; VIANELLO, R. P.; VALDISSER, P. A. M. R.; NASCIMENTO, M.
We aimed to apply genomic information based on SNP (single nucleotide polymorphism) markers for the genetic evaluation of the traits ?stay-green? (SG), plant architecture (PA), grain aspect (GA) and grain yield (GY) in common bean through Bayesian models. These models were compared in terms of prediction accuracy and ability for heritability estimation for each one of the mentioned traits. A total of 80 cultivars were genotyped for 377 SNP markers, whose effects were estimated by five different Bayesian models: Bayes A (BA), B (BB), C (BC), LASSO (BL) e Ridge regression (BRR). Although, prediction accuracies calculated by means of cross-validation have been similar within each trait, the BB model stood out for the trait SG, whereas the BRR was indicated...
Tipo: Artigo de periódico
Palavras-chave: Validação cruzada; Cross-validation; Feijão; Phaseolus Vulgaris; Marcador Molecular; Beans; Genetic markers; Marker-assisted selection.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095835

Tagging microsatellite marker to a blast resistance gene in the irrigated rice cultivar Cica-8.
Provedor de dados: 119
Autores: PINHEIRO, T. M.; ARAÚJO, L. G. de; SILVA-LOBO, V. L. da; PRABHU, A. S.; FILIPPI, M. C. de.
The rice cultivar Cica-8 exhibit differential reaction to several pathotypes of Magnaporthe oryzae. The objective of the present investigation was to determine the number of alleles involved in the expression of resistance to leaf blast and identify microsatellite markers linked to these alleles. A cross between cultivar Metica-1 and Cica-8 susceptible and resistant, respectively, to pathotype IB-1 (Py1049) was made to obtain F1, F2, BC1:1 and BC1:2 progenies. Greenhouse tests for leaf blast reaction showed that resistance is controlled by a monogenic dominant gene. For testing microsatellite markers, DNA of both resistant and susceptible parents and F1 and F2 populations was extracted. As expected for single dominant gene the F2 populations segregated at...
Tipo: Artigo de periódico
Palavras-chave: Arroz; Oryza sativa; Brusone; Rice; Magnaporthe grisea; Marker-assisted selection; Genetic markers.
Ano: 2012
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/933575

A mixed model to multiple harvest-location trials applied to genomic prediction in Coffea canephora.
Provedor de dados: 119
Autores: FERRÃO, L. F. V.; FERRÃO, R. G.; FERRAO, M. A. G.; FONSECA, A. F. A. da; GARCIA, A. A. F.
Genomic selection (GS) has been studied in several crops to increase the rates of genetic gain and reduce the length of breeding cycles. Despite its relevance, there are only a modest number of reports applied to the genus Coffea. Effective implementation depends on the ability to consider genomic models, which correctly represent breeding scenario in which the species are inserted. Coffee experimentation, in general, is represented by evaluations in multiple locations and harvests to understand the interaction and predict the performance of untested genotypes. Therefore, the main objective of this study was to investigate GS models suitable for use in Coffea canephora. An expansion of traditional GBLUP was considered and genomic analysis was performed...
Tipo: Artigo de periódico
Palavras-chave: Genotyping-by-sequencing; GBLUP; Multi-environment trials; Perennial crops; Marker-assisted selection.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081803

Genomic prediction in contrast to a genome-wide association study in explaining heritable variation of complex growth traits in breeding populations of Eucalyptus.
Provedor de dados: 119
Autores: MÜLLER, B. S. F.; NEVES, L. G.; ALMEIDA FILHO, J. E. de; RESENDE JUNIOR, M. F. R.; MUÑOZ, P. R.; SANTOS, P. E. T. dos; PALUDZYSZYN FILHO, E.; KIRST, M.; GRATTAPAGLIA, D.
Background: The advent of high-throughput genotyping technologies coupled to genomic prediction methods established a new paradigm to integrate genomics and breeding. We carried out whole-genome prediction and contrasted it to a genome-wide association study (GWAS) for growth traits in breeding populations of Eucalyptus benthamii (n =505) and Eucalyptus pellita (n =732). Both species are of increasing commercial interest for the development of germplasm adapted to environmental stresses. Results: Predictive ability reached 0.16 in E. benthamii and 0.44 in E. pellita for diameter growth. Predictive abilities using either Genomic BLUP or different Bayesian methods were similar, suggesting that growth adequately fits the infinitesimal model. Genomic...
Tipo: Artigo de periódico
Palavras-chave: Genomic selection; GWAS; SNP genotyping; Seleção genômica; Espécie exótica.; Eucalipto; Melhoramento genético vegetal.; Eucalyptus benthamii; Eucalyptus pellita; Marker-assisted selection; Plant breeding; Genetic relationships.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080081

Validação de marcadores moleculares associados a resistência a doenças emergentes de arroz.
Provedor de dados: 119
Autores: SOARES, S. da S.; SILVA, J. N.; RODRIGUES, L. A.; COSTA, S. de P. P.; ABREU, A. G. de.
O objetivo do trabalho foi validar marcadores moleculares que estão associados a QTLs de resistência à mancha parda e queima das bainhas em genótipos de arroz para serem usados na seleção assistida por marcadores. O presente estudo foi conduzido no Laboratório de Seleção Assistida da Embrapa Arroz e Feijão. Inicialmente foram identificados na literatura 16 marcadores microssatélites próximos a QTLs de resistência à mancha parda e queima da bainha.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Arroz; Oryza Sativa; Marcador Molecular; Resistência; Rice; DNA primers; Marker-assisted selection.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118019

Desenvolvimento de marcadores associados à resistência do bicho-mineiro: seleção e validação de SNPs.
Provedor de dados: 119
Autores: BAZIOLI, J. M.; GUIMARÃES, P. de S.; STRAZZA, B. A.; SCHENK, J. C. M.; PADILHA, L.; GUERREIRO-FILHO, O.; MALUF, M. P.
O objetivo deste estudo é o desenvolvimento de marcadores do tipo SNPs associados com a resistência ao bicho-mineiro em café. A estratégia principal utilizou as análises genômicas de microarranjo, para caracterização de expressão diferencial de genes-candidatos em plantas resistentes infestadas com o bicho-mineiro. Inicialmente, análises in silico de 2137 destes genes identificaram aqueles potencialmente relacionados com a especificidade da resposta de defesa da planta. Uma vez selecionados, o perfil de expressão de 22 genes foi confirmado por qRT-PCR em experimentos utilizando plantas resistentes e suscetíveis, infectadas pelo bicho-mineiro. Para busca de polimorfismos do tipo SNPs, que poderão ser utilizados como marcadores para seleção-assistida,...
Tipo: Anais e Proceedings de eventos
Palavras-chave: Seleção-assistida; Estresse biótico; Melhoramento molecular; Molecular breeding; Marker-assisted selection; Biotic stress.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113382

Genomic selection in tropical forage grasses: current status and future applications.
Provedor de dados: 119
Autores: SIMEÃO, R. M.; RESENDE, M. D. V. de; ALVES, R. S.; PESSOA-FILHO, M.; AZEVEDO, A. L. S.; JONES, C. S.; PEREIRA, J. F.; MACHADO, J. C.
The world population is expected to be larger and wealthier over the next few decades and will require more animal products, such as milk and beef. Tropical regions have great potential to meet this growing global demand, where pasturelands play a major role in supporting increased animal production. Better forage is required in consonance with improved sustainability as the planted area should not increase and larger areas cultivated with one or a few forage species should be avoided. Although, conventional tropical forage breeding has successfully released well-adapted and high-yielding cultivars over the last few decades, genetic gains from these programs have been low in view of the growing food demand worldwide. To guarantee their future impact on...
Tipo: Artigo de periódico
Palavras-chave: Seleção assistida; Seleção genômica; Brachiaria; Apomixia; Poliploidia; Gramínea; Apomixis; Marker-assisted selection; Polyploidy.
Ano: 2021
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133333

QTLs for resistance to soybean cyst nematode, races 3, 9, and 14 in cultivar Hartwig.
Provedor de dados: 119
Autores: FERREIRA, M. F. da; CERVIGNI, G. D. L; FERREIRA, A.; SCHUSTER, I.; SANTANA, F. A.; PEREIRA, W. D.; BARROS, E. G. de; MOREIRA, M. A.
The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is...
Tipo: Artigo de periódico
Palavras-chave: Seleção assistida por marcador; Marcador molecular; Melhoramento; Glycine Max; Breeding; Genetic markers; Marker-assisted selection.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/910178

Seleção genômica ampla (GWS) e associação genômica ampla (GWAS) no melhoramento de forrageiras: abordagem conceitual, genética quantitativa e aplicações.
Provedor de dados: 119
Autores: MIQUELONI, D. P.; RESENDE, R. M. S.; ASSIS, G. M. L. de.
Atualmente, desenvolvimento da tecnologia de análise genômica de alta resolução e alto rendimento tem a capacidade de gerar informações sobre milhões de pares de bases em um único ensaio, permitindo a detecção de variação em um único nucleotídeo de uma sequência de DNA. Este contexto possibilitou o surgimento de novas abordagens para a seleção assistida por marcadores, dentre elas os estudos de associação genômica ampla (GWAS), que visam o mapeamento genético por meio das associações entre os locos e a característica fenotípica na população e buscam detectar efeitos dos genes sobre os valores genéticos dos indivíduos; e a seleção genômica ampla (GWS), que detecta genótipos favoráveis por meio de informações genotípicas para inferir sobre os valores...
Tipo: Artigo de periódico
Palavras-chave: Associação genômica ampla (GWAS); Seleção genômica ampla (GWS); Fitomejoramiento; Selección asistida por marcadores; Melhoramento Genético Vegetal; Seleção Genética; Marcador Genético; Plant breeding; Genetic markers; Marker-assisted selection.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118058

SNP discovery in three South American freshwater characiformes species by deep sequencing of Reduced Representation Libraries (RRL).
Provedor de dados: 119
Autores: IANELLA, P.; VARELA, E. S.; VILLELA, L. C. V.; YAMAGISHI, M. E. B.; OLIVEIRA, F. S. de; PAIVA, S. R.; CAETANO, A. R.
This represents the first report of a large-scale effort to identify SNP markers for Pacu and Pirapitinga species, useful for prospecting species-specific SNPs for species-purity certification of commercial Tambaqui broodstocks.
Tipo: Anais e Proceedings de eventos
Palavras-chave: X; Colossoma Macropomum; Tambaqui; Freshwater fisheries; Fish; Marker-assisted selection; Characiformes.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118074

Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle.
Provedor de dados: 119
Autores: GOMES, R. da C.; SILVA, S. L.; CARVALHO, M. E.; REZENDE, F. M.; PINTO, L. F. B.; SANTANA, M. H. A.; STELLA, T. R.; MEIRELLES, F. V.; ROSSI JÚNIOR, P.; LEME, P. R.; FERRAZ, J. B. S.
We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed conversion ratio, partial efficiency of growth, residual feed intake (RFI), ultrasound backfat, rump fat, and ribeye area.
Tipo: Artigo de periódico
Palavras-chave: Molecular breeding value; Residual feed intake; Protein turnover.; Carcaça; Síntese de proteína; Bos Indicus.; Marker-assisted selection; Protein synthesis.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/972453

Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance.
Provedor de dados: 119
Autores: FRITSCHE-NETO, R.; SOUZA, T. L. P. O. de; PEREIRA, H. S.; FARIA, L. C. de; MELO, L. C.; NOVAES, E.; BRUM, I. J. B.; JANNINK, J-L.
Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80 % and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to...
Tipo: Artigo de periódico
Palavras-chave: Feijão; Phaseolus Vulgaris; Antracnose; Resistência; Mancha Angular; Anthracnose; Beans; Colletotrichum lindemuthianum virus; Pseudocercospora; Disease resistance; Marker-assisted selection.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1111232

Estudo de associação de polimorfismos nos genes LEP e TG com características de qualidade de carne e carcaça em bovinos da raça Canchim.
Provedor de dados: 119
Autores: SOUSA, I. I.; BLECHA, I. M. Z.; MACIEL, S. da S.; FERREIRA, A. B. R.; FEIJO, G. L. D.; FAVERO, R.; SANTIAGO, G. G.; SIQUEIRA, F.
Avaliou-se os marcadores moleculares do tipo SNP (Single-nucleotide Polymorphism) E2FB (AY138588.1:c.305C&gt;T) e TG5 (X05380.1:g.-422C&gt;T) nos genes da leptina e da tireoglobulina em animais da raça Canchim. As genotipagens foram realizadas por PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) e as frequências alélicas e genotípicas foram comparadas pelo teste Quiquadrado. Os desvios de frequências observadas em relação às esperadas para o Equilíbrio de Hardy-Weinberg foram analisados a 5% de significância e os dados foram submetidos à ANOVA por meio do PROC GLM (SAS). No marcador LEP/Kpn2I observou-se associação sugestiva (P=0,0555) para área de olho de lombo e marmoreio (P=0,068), porém não foi verificado efeito...
Tipo: Anais e Proceedings de eventos
Palavras-chave: Seleção assistida por marcadores; Frequência alélica; Teste Quiquadrado; Marcador moleculr.; Gado de corte; Melhoramento genético animal.; Beef cattle; Genetic markers; Genetic improvement; Marker-assisted selection; Single nucleotide polymorphism.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077308

Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 genes in beef cattle.
Provedor de dados: 119
Autores: BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F.
The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A&gt;G (CAST/DdeI) and AF248054.2:g.6545C&gt;T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an...
Tipo: Artigo de periódico
Palavras-chave: Animal breeding; Marker-assisted selection; Meat tenderness.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117678

Regularized quantile regression applied to genome-enabled prediction of quantitative traits.
Provedor de dados: 119
Autores: NASCIMENTO, M.; SILVA, F. F. e; RESENDE, M. D. V. de; CRUZ, C. D.; NASCIMENTO, A. C. C.; VIANA, J. M. S.; AZEVEDO, C. F.; BARROSO, L. M. A.
Genomic selection (GS) is a variant of marker-assisted selection, in which genetic markers covering the whole genome predict individual genetic merits for breeding. GS increases the accuracy of breeding values (BV) prediction. Although a variety of statistical models have been proposed to estimate BV in GS, few methodologies have examined statistical challenges based on non-normal phenotypic distributions, e.g., skewed distributions. Traditional GS models estimate changes in the phenotype distribution mean, i.e., the function is defined for the expected value of trait-conditional on markers, E(Y|X). We proposed an approach based on regularized quantile regression (RQR) for GS to improve the estimation of marker effects and the consequent genomic estimated...
Tipo: Artigo de periódico
Palavras-chave: Seleção genômica; Genomic selection; Regularized regression; SNP effects; Estatística; Marker-assisted selection; Simulation models; Statistics.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084109

Melhoramento genético como ferramenta para o crescimento e o desenvolvimento da ovinocultura de corte.
Provedor de dados: 119
Autores: LOBO, R. N. B.; LOBO, A. M. B. O.
Resumo: Dentre as ações relacionadas à promoção do crescimento e do desenvolvimento de uma atividade pecuária, o melhoramento genético é uma das que mais merece atenção. Para que a ovinocultura de corte no Brasil possa se desenvolver, são necessárias a seleção e a multiplicação de genótipos apropriados aos diversos ecossistemas encontrados no país. Com a finalidade de aperfeiçoar a capacidade de produção dos animais, as ferramentas básicas do melhoramento genético são a seleção e os métodos de acasalamento. Neste trabalho são apresentadas algumas definições fundamentais do melhoramento genético e discutidas formas de sua contribuição para o crescimento da ovinocultura de corte. [Animal breeding as a tool for upgrowth and development of meat sheep sector]....
Tipo: Artigo de periódico
Palavras-chave: Ovino de corte; Genética quantitativa; Molecular markers; Productivity.; Genética Molecular; Marcador Molecular; Melhoramento Genético Animal; Produtividade; Seleção.; Sheep meat; Genetic markers; Marker-assisted selection; Quantitative genetics.
Ano: 2007
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/532599

Prospects for using conventional techniques and molecular biological tools to enhance efficiency of crop plants in low-nutrient environments: QTL mapping and mas for improved phosphorus uptake.
Provedor de dados: 119
Autores: HASH, C. T.; SCHAFFERT, R. E.; PEACOCK, J. M.
Tipo: Parte de livro
Palavras-chave: Pearl millet; Chickpea; Pigeonpea.; Fósforo; Grão de Bico; Guandu; Milheto; Raiz.; Marker-assisted selection; Phosphorus; Roots.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/485037

Accuracy of genomic prediction for tick resistance in Braford and Hereford cattle.
Provedor de dados: 119
Autores: CARDOSO, F. F.; SOLLERO, B. P.; GOMES, C. C. G.; COMIN, H. B.; ROSO, V. M.; HIGA, R. H.; YOKOO, M. J.; CAETANO, A. R.; AGUILAR, I.
This work aimed to determine the accuracy and bias of genomic predictions of Braford (BO) and Hereford (HH) cattle genetic resistance to ticks.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Seleção genômica; Resistência a carrapatos; Tick resistance; Gado de corte; Beef cattle; Marker-assisted selection.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1003697

Genomic prediction in contrast to a genome-wide association study in explaining heritable variation of complex growth traits in breeding populations of Eucalyptus.
Provedor de dados: 119
Autores: MÜLLER, B. S. F.; NEVES, L. G.; ALMEIDA FILHO, J. E. de; RESENDE JUNIOR, M. F. R.; MUÑOZ, P. R.; SANTOS, P. E. T. dos; PALUDZYSZYN FILHO, E.; KIRST, M.; GRATTAPAGLIA, D.
Background: The advent of high-throughput genotyping technologies coupled to genomic prediction methods established a new paradigm to integrate genomics and breeding. We carried out whole-genome prediction and contrasted it to a genome-wide association study (GWAS) for growth traits in breeding populations of Eucalyptus benthamii (n =505) and Eucalyptus pellita (n =732). Both species are of increasing commercial interest for the development of germplasm adapted to environmental stresses. Results: Predictive ability reached 0.16 in E. benthamii and 0.44 in E. pellita for diameter growth. Predictive abilities using either Genomic BLUP or different Bayesian methods were similar, suggesting that growth adequately fits the infinitesimal model. Genomic...
Tipo: Separatas
Palavras-chave: Genomic selection; GWAS; Seleção genômica; SNP genotyping; Espécie exótica; Eucalipto; Melhoramento genético vegetal; Eucalyptus benthamii; Eucalyptus pellita; Genetic relationships; Marker-assisted selection; Plant breeding.
Ano: 2017
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080149

Selection of resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening.
Provedor de dados: 119
Autores: VIEIRA, A. F.; ALMEIDA, L. C. S.; RODRIGUES, L. A.; COSTA, J. G. C.; MELO, L. C.; PEREIRA, H. S.; SANGLARD, D. A.; SOUZA, T. L. P. O.
The main goal of this work was to select resistance sources to common bean anthracnose by field phenotyping and DNA marker-assisted screening. Fifty-five common bean genotypes, including differential varieties, characterized resistance sources, elite lines, cultivars and controls, were evaluated in a field inoculation trial and screened with SCAR markers linked to resistance genes that are important in Brazil. The field trial was carried out in Santo Antônio de Goiás, GO, Brazil, during the fall/winter growing season of 2014, using artificial inoculation with a mixture of six races of Colletotrichum lindemuthianum, selected based on their high virulence and prevalence in Brazil. Amplification reactions with the SCAR markers previously identified as linked...
Tipo: Artigo de periódico
Palavras-chave: Feijão; Phaseolus Vulgaris; Resistência Genética; Antracnose; DNA; Marcador Molecular; Beans; Anthracnose; Marker-assisted selection; Genetic resistance; Plant breeding.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094367

Genetic linkage map and mapping of the locus of biological nitrogen fixation inefficiency in cowpea
Provedor de dados: 1
Autores: Seido, Sirando Lima; Fernandes Santos, Carlos Antonio.
The objectives of the present study were to construct a cowpea genetic map using the F2 population resulting from the cross IC-1 x BRS Marataoã, based on single nucleotide polymorphism (SNP) markers, and to map the cpi gene, with additional reference to introgression with the consensus map of species, aiming to identify markers for assisted selection to develop more efficient cultivars for BNF. The parents and 89 F2 plants were genotyped with 51,128 SNP markers, of which 910 polymorphic markers were used to construct the map. The results revealed 11 linkage groups, with an average of 82 markers per chromosome and average distance of 1.26 cM between markers. Recombination analysis of the SNPs indicated that markers 2_12850 and 2_00188, located in linkage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vigna unguiculata; Nitrogen fixation; Marker-assisted selection.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42603

Genetics of coffee quality
Provedor de dados: 61
Autores: Leroy,Thierry; Ribeyre,Fabienne; Bertrand,Benóit; Charmetant,Pierre; Dufour,Magali; Montagnon,Christophe; Marraccini,Pierre; Pot,David.
Coffee quality, in the present context of overproduction worldwide, has to be considered as a main selection criterion for coffee improvement. After a definition of quality, and an overview of the non genetic factors affecting its variation, this review focuses on the genetic factors involved in the control of coffee quality variation. Regarding the complexity of this trait, the different types of quality are first presented. Then, the great variation within and between coffee species is underlined, mainly for biochemical compounds related to quality (caffeine, sugars, chlorogenic acids, lipids). The ways for breeding quality traits for cultivated species, Coffea arabica and Coffea canephora are discussed, with specific challenges for each species. For C....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea spp.; Biochemical compounds; Candidate genes; ESTs; Genetic breeding; Marker-assisted selection; Quality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100016

Improving durum wheat (Triticum turgidum L. var durum) grain yellow pigment content through plant breeding
Provedor de dados: 118
Autores: Schulthess,Albert; Schwember,Andrés R.
A. Schulthess, and A.R. Schwember. 2013. Improving durum wheat (Triticum turgidum L. var durum) grain yellow pigment content through plant breeding. Cien Inv. 40(3): 475-490. Wheat grain yellow pigment content (GYPC) is an important trait that determines pasta quality. The main objective of this review is to examine the genetics regulating GYPC to enhance it through breeding, leading to improved pasta quality. Although GYPC is a polygenic trait, its high heritability has facilitated breeding internationally. GYPC is influenced by one or two major loci with additive effects plus several minor genes, and there is evidence showing that the phytoene synthase loci PSY1A and PSY1B are strong candidate genes that regulate GYPC. Nine Chilean durum wheat (Triticum...
Tipo: Journal article
Palavras-chave: Durum wheat; Grain yellow pigment content; Improved color; PSY1A; PSY1B; Backcrossing; Marker-assisted selection.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300002

Diversity of microsatellites linked to the FSHbeta gene, their usefulness for individual identification and association with reproductive performance
Provedor de dados: 65
Autores: Duarte,Letícia Becker Homrich; Moraes,José Carlos Ferrugem; Weimer,Tania de Azevedo.
The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellites; Bovine genetic diversity; Individual identification; Reproductive performance; Marker-assisted selection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023

Mapping aluminum tolerance loci in cereals: A tool available for crop breeding
Provedor de dados: 69
Autores: Inostroza-Blancheteau,Claudio; Soto,Braulio; Ibáñez,Cristian; Ulloa,Pilar; Aquea,Felipe; Arce-Johnson,Patricio; Reyes-Díaz,Marjorie.
Aluminum (Al) toxicity is the main factor limiting crop productivity in acidic soils around the world. In cereals, this problem reduces crop yields by 30-40%. The use of DNA-based markers linked to phenotypic traits is an interesting alternative approach. Strategies such as molecular marker-assisted selection (MAS) in conjunction with bioinformatics-based tools such as graphical genotypes (GGT) have been important for confirming introgression of genes or genomic regions in cereals but also to reduce the time and cost of identifying them through genetic selection. These biotechnologies also make it possible to identify target genes or quantitative trait loci (QTL) that can be potentially used in similar crops to increase their productivity. This review...
Tipo: Journal article
Palavras-chave: Aluminum tolerance; ALMT1; Cereals; Marker-assisted selection; Organic acid.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400011

Development and application of high-throughput amplified fragment length polymorphism technique in Calluna vulgaris (Ericaceae)
Provedor de dados: 69
Autores: Borchert,Thomas; Gawenda,Inka.
Calluna vulgaris is an important ornamental crop of the horticultural industry in Europe. In order to improve breeding of this species, especially of the most important trait of &amp;#8216;bud-flowering', the implementation of molecular techniques that allow rapid, reproducible and efficient screening of whole segregating populations e.g. for molecular marker and mapping approaches is a requirement. We therefore aimed to introduce the powerful tool of amplified fragment length polymorphisms (AFLP®), a widely and successfully applied method, into our methodological assortment. As an essential prerequisite, the isolated DNA should be of adequate quality which is a common obstacle when dealing with woody species and their interfering secondary...
Tipo: Journal article
Palavras-chave: DNA content; Mapping; Marker-assisted selection; Ornamental; Plant tissue.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200007

Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus)
Provedor de dados: 74
Autores: Nascimento,Carlos Souza do; Machado,Marco Antonio; Martinez,Mario Luiz; Silva,Marcos Vinícius G. Barbosa da; Guimarães,Marta Fonseca Martins; Campos,Ana Lúcia; Azevedo,Ana Luisa Sousa; Teodoro,Roberto Luiz; Verneque,Rui da Silva; Guimarães,Simone Eliza Facioni; Oliveira,Denise Aparecida Andrade.
The effect of the bovine major histocompatibility complex (BoLA) locus on animal health may be due to a direct action of its alleles on immune functions, whereas its indirect effect on production traits might be explained by the better general health conditions of more productive animals. In the present study, the BoLA-DRB3 gene was investigated in 1058 cows belonging to seven Brazilian Gyr Dairy herds (Bos indicus, Zebu cattle). A total of 37 alleles were identified, 15 of them described for the first time in a Zebu breed. A highly significant association (p &lt; 0.02) was observed between allele *54 and a decrease (-26.1 kg) in milk protein yield and there was a significant association (p &lt; 0.05) between this allele and lower (-26.07 kg) milk fat...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Marker-assisted selection; Molecular markers; PCR-RFLP.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400011

Microsatellite-assisted backcross selection in maize
Provedor de dados: 74
Autores: Benchimol,Luciana Lasry; Souza Jr.,Cláudio Lopes de; Souza,Anete Pereira de.
A microsatellite marker (SSR) was chosen to simulate a target allele and three criteria (02, 04 and 06 markers per chromosome) were tested to evaluate the most efficient parameters for performing marker-assisted backcross (MAB) selection. We used 53 polymorphic SSRs to genotype 186 BC1 maize (Zea mays L.) plants produced by crossing the inbred maize lines L-08-05 (donor parent) and L-14-4B (recurrent parent). The second backcross (BC2) generation was produced with 180 plants and screened with markers which were not recovered from the first backcross (BC1) generation. A total of 480 plants were evaluated in the third backcross (BC3) generation from which 48 plants were selected for parental genotype recovery. Recurrent genotype recovery averages in three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maize; Marker-assisted backcrossing (MAB); Marker-assisted selection; Microsatellites.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500022

Interaction between estrogen receptor and retinol-binding protein-4 polymorphisms as a tool for the selection of prolific pigs
Provedor de dados: 74
Autores: Gonçalves,Iara Denise Vasconcellos; Gonçalves,Paulo Bayard Dias; Silva,Janandra Cortese da; Portela Jr,Valério Valdetar; Borges,Luiz Felipe Kruel; Oliveira,João Francisco Coelho; Lovatto,Paulo Alberto.
The aim of the present study was to investigate the association of the estrogen receptor (ER-PvuII) and retinol-binding protein 4 (RBP4-MspI) gene polymorphisms and their interactions with prolificacy in a commercial synthetic pig line reared in Brazil. A total of 10,374 piglet records from 218 sows and 817 litters were used for litter size analysis. Only females with three or four farrowings were included in the analysis. The mean litter size ranged from 5.0 to 19.5 piglets. DNA was extracted from leukocytes by a standard method, and ER-PvuII and RBP4-MspI polymorphisms were characterized by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The association between alleles or genotypes and reproductive performance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Marker-assisted selection; Retinol-binding protein-4; Estrogen receptor.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300014

A deterministic simulation study of embryo marker-assisted selection for age at first calving in Nellore (Bos indicus) beef cattle
Provedor de dados: 74
Autores: Rosa,Artur J.M.; Bijma,Piter; Oliveira,Henrique N.; Lôbo,Raysildo B.; van Arendonk,Johan A.M.
We used deterministic simulation of four alternative multiple ovulation and embryo manipulation (MOET) closed nucleus schemes to investigate the benefits of using marker-assisted selection (MAS) of Nellore (Bos indicus) beef cattle embryos prior to transplantation to reduce the age at first calving (AFC). We found that MAS resulted in increased genetic gain as compared to selection without AFC quantitative trait loci (AFC-QTL) information. With single-stage selection the genetic response (GR) increased as follows: GR = 0.68% when the AFC-QTL explained 0.02 of the AFC additive genetic variance (sigma2A); GR = 1.76% for AFC-QTL explaining 0.05 sigma2A; GR = 3.7% for AFC-QTL explaining 0.1 sigma2A; and GR = 55.76% for AFC-QTL explaining 0.95 sigma2A. At the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Age at first calving; Beef cattle; Marker-assisted selection; Pre-selection; Quantitative trait loci.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400008

Detection of a novel quantitative trait locus for cold tolerance at the booting stage derived from a tropical japonica rice variety Silewah
Provedor de dados: 19
Autores: Mori, Masahiko; Onishi, Kazumitsu; Tokizono, Yoshiro; Shinada, Hiroshi; Yoshimura, Toru; Numao, Yoshinori; Miura, Hideho; Sato, Takashi; 大西, 一光; 三浦, 秀穂.
Cold stress at the booting stage in rice induces spikelet sterility because of aberrant microspore development, which often seriously damages seed production. Some breeding lines with high cold tolerance were developed by using tropical japonica variety Silewah as a donor of cold tolerance; however, the genetic factors that confer cold tolerance of this variety have not been comprehensively analyzed. In this study, phenotypic and molecular characterization of novel cold-tolerant strains derived from crosses with Silewah was performed to identify quantitative trait loci (QTLs) responsible for cold tolerance. Molecular marker analysis revealed that 2 cold-tolerant strains carried chromosomal segments of Silewah at the same genomic regions on chromosomes 3, 4...
Palavras-chave: Oryza sativa; Cold tolerance; Booting stage; QTL; Phenotypic selection; Marker-assisted selection; Exotic germplasm.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3040