Consulta: subjectFacets:"Mass spectrometry"
Registros recuperados: 47
Data/hora: 05/06/2024 12:20:16

Fertilización foliar y su ruta de penetración en la orquídea Cymbidium sp.
Provedor de dados: 32
Autores: García Gaytán, Víctor.
La presente investigacion se dividió en tres experimentos, dos realizados en el Colegio de Postgraduados en Ciencias Agrícolas, Campus Montecillo Edo. México y la tercera en la Facultad de Agrobiología “Presidente Juárez” Uruapan Michoacan. Dada la importancia de la fertilización foliar  como práctica común en los medios de producción y escasa información disponible sobre la absorción de nutrientes a nivel foliar en orquídeas, en el primer estudio se caracterizó la anatomía foliar de Cymbidium sp. e dentificó las rutas de penetración de un fertilizante foliar 10-10-10 NPK. En esta etapa se encontró que el grosor de la cutícula varía a lo largo de la hoja. La porción media fue más gruesa (29.90 µm) que la apical y basal (26.91y 25.75 µm). El número de...
Palavras-chave: Fertilización foliar; Cromatografía en capa fina; Espectrometría de masas; Pseudobulbos escapo; Foliar feeding; Thin layer chromatography; Mass spectrometry; Pseudobulbos scapes; Edafología; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/2042

Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Provedor de dados: 74
Autores: Leonardi,Gabriela de Almeida; Carlos,Natália Aparecida; Mazzafera,Paulo; Balbuena,Tiago Santana.
Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Mass spectrometry; Omics; Proteomics.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191

Protein profile of rice (Oryza sativa) seeds
Provedor de dados: 74
Autores: Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping.
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012

Determinação de resíduos de paclobutrazol em manga (Mangifera indida L.) por cromatografia acoplada a espectometria de massas (LC-MS/MS).
Provedor de dados: 14
Autores: FERRACINI, V. L.; QUEIROZ, S. C. do N. de; ROSA, M. A.; LOPES, P. R. C.
Paclobutrazol (PBZ) é um regulador de crescimento que tem sido utilizado para alterar o ciclo fenológico da mangueira visando a produção de frutos em qualquer época do ano. É bastante utilizado na região nordeste do Brasil e a determinação dos resíduos na fruta é importante pois há pouco conhecimen- to sobre o acúmulo desta substância na planta em condições brasileiras. Para isso foi necessário adaptar um método analítico para determinar resídu- os de paclobutrazol em frutos de manga. Após a extração do PBZ na matriz o extrato foi analisado por LC-MS/MS. Os limites de detecção (LOD) e de quantificação (LOQ) obtidos para o PBZ foram de 0,25 ?g Kg-1 e 1,25 ?g Kg-1, respectivamente. As recuperações foram de 82 a 94% e se encontram dentro da faixa considerada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: LC-MS/MS; Resíduos de paclobutrazol; Especrometria.; Resíduo químico; Regulador de crescimento; Análise química; Cromatografia; Manga.; Paclobutrazol; Chemical residues; Chemical analysis; Chromatography; Mass spectrometry.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/898423

Método analítico multirresíduo para determinação de agrotóxicos e seus produtos de degradação em águas superficiais.
Provedor de dados: 14
Autores: SCORZA JUNIOR, R. P.; PAZ, R. B. da S.
bitstream/item/217875/1/BP-86-2020.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Validação de método; Monitoramento; Method validation; Resíduo Agrícola; Agrotóxico; Cromatografia; Espectrometria; Pesticide residues; Liquid chromatography; Mass spectrometry; Monitoring.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1126682

Dissipação de permetrina em pepino utilizando princípios de boas práticas agrícolas e de laboratório.
Provedor de dados: 14
Autores: BARIZON, R. R. M.; FERRACINI, V. L.; QUEIROZ, S. C. do N. de; SOUZA, M. D. de; SPADOTTO, C. A.; SOUZA, D. R. C. de.
Resumo - Este trabalho teve como objetivo realizar testes de campo e de laboratório, utilizando os princípios das boas práticas de laboratório (BPL) e boas práticas agrícolas (BPA). Esses princípios foram criados para harmonizar procedimentos e garantir a aceitação dos resultados em diferentes países e regiões da Organização para Cooperação e Desenvolvimento Econômico (OCDE), além de serem obrigatórios para registro de agrotóxicos. Para adequar à instalação de teste desses princípios foi realizado um estudo de campo com aplicação de permetrina no cultivo de pepino, que é considerado uma cultura com suporte fitossanitário insuficiente (ou minor crops). Este pesticida foi selecionado por duas razões: (a) vem sendo detectado nos programas de monitoramento, e...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Boas práticas de laboratório; Boas práticas agrícolas; Permetrina; GC-MS/MS; Espectrometria de massas; Agrotóxico; Espectrometria; Análise Química; Pepino; Cucumis; Pesticides; Good laboratory practices; Good agricultural practices; Mass spectrometry.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111785

Determinação de agrotóxicos e ocratoxina A em café arábica sob diferentes níveis de torrefação.
Provedor de dados: 14
Autores: ROSA, J. S. da; FREITAS-SILVA, O.; CAMPOS, R. da S.; CASTRO, I. M. de; TEIXEIRA, A. da S.
O café é a segunda maior commodity do mundo e tamanha importância faz do café uma matriz alimentar muito estudada. A necessidade de incremento na produtividade dessa cultura, porém, pode aumentar o uso de alguns agrotóxicos em sistemas de produção de café. Da mesma forma, as práticas de colheita e processamento pós-colheita do café estão diretamente relacionadas à contaminação dos grãos por fungos micotoxigênicos. Rastrear a presença destes dois tipos de contaminantes em amostras de café Arábica comum (com diferentes teores de grãos defeituosos) e submetidos a diferentes níveis de torra foi o objetivo deste trabalho. Os agrotóxicos foram analisados pelo método multirresíduo, por espectrometria de massas sequencial, e a ocratoxina A, por cromatografia...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Contaminante; Cromatografia líquida de alta eficiência; Micotoxina; Coffea Arábica; Tecnologia de Alimento; Espectrometria; Cromatografia; Food technology; Vegetable products; High performance liquid chromatography; Mycotoxins; Mass spectrometry; Poisoning; Agrochemicals.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112112

Convenient preparation of picolinyl derivatives from fatty acid esters
Provedor de dados: 5
Autores: Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal.
It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation....
Tipo: Text
Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf

Zinc isotopes as tracers of anthropogenic sources and biogeochemical processes in contaminated mangroves
Provedor de dados: 5
Autores: Ferreira Araujo, Daniel; Machado, Wilson; Weiss, Dominik; Mulholland, Daniel S.; Garnier, Jeremie; Souto-oliveira, Carlos E.; Babinski, Marly.
Recent work has shown that variations in zinc (Zn) isotope ratios enable us to identify contamination sources in the terrestrial environment and uptake processes in higher plants. Here in this study, we demonstrate that this also holds true for mangrove forests, which play an important role in the biogeochemical cycling of metals in tropical coastal ecosystems and that are seriously threatened by anthropogenic pollution. To this end, we determined zinc concentration and isotope composition (expressed using the δ66Zn notation relative to the JMC 3-0749-L standard) in sediments and tree leaves collected from a mangrove close to Rio de Janeiro in Brazil. The δ66ZnJMC values of sediments vary between +0.36 and + 0.84‰ and fall on a mixing line between detrital...
Tipo: Text
Palavras-chave: Metal transition isotopes; Metal contamination; Mass spectrometry; Coastal ecosystems; Mangrove leaves.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00440/55196/56660.pdf

Criblage bioguidé et analyse CL - SMHR des toxines et d'au tres métabolites d'intérêt chez les dinoflagellés G ambierdiscus et Fukuyoa
Provedor de dados: 5
Autores: Pisapia, Francesco.
The epi-benthic dinoflagellates Gambierdiscus and Fukuyoa produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. CTXs are bio-accumulated and bio-transformed along the marine food chain and cause Ciguatera Fish Poisoning (CFP), the most common non-bacterial foodborne intoxication worldwide. Recently, CFP has been reported from areas previously not considered endemic, namely the Canary Islands, and an increasing number of species has been discovered in these genera. Little is known about the relative toxicity between different species and the toxin congeners produced by these dinoflagellates, especially by strains outside the Pacific Ocean. Several strains of Gambierdiscus and Fukuyoa were screened for their...
Tipo: Text
Palavras-chave: Ciguatéra; Gambierdiscus; Fukuyoa; Ciguatoxines; Maïtotoxines; Test neuro-2a; Test hémolytique; Spectrométrie de masse.; Keywords: Ciguatera Fish Poisoning; Gambierdiscus; Fukuyoa; Ciguatoxins; Maitotoxins; Neuro-2a assay; Hemolytic assay; Mass spectrometry.
Ano: 2017
URL: https://archimer.ifremer.fr/doc/00415/52635/53481.pdf

Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity
Provedor de dados: 5
Autores: Cacas, Jean-luc; Bure, Corinne; Furt, Fabienne; Maalouf, Jean-paul; Badoc, Alain; Cluzet, Stéphanie; Schmitter, Jean-marie; Antajan, Elvire; Mongrand, Sébastien.
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots,...
Tipo: Text
Palavras-chave: Glycosphingolipid; Glycosyl-Inositol-Phospho-Ceramide; GIPC; Mass spectrometry; MALDI-TOF; Polar head; Roots; Photosynthetic tissues; Berries.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00166/27689/25884.pdf

A di-O-dihydrogeranylgeranyl glycerol from Thermococus S 557, a novel ether lipid, and likely intermediate in the biosynthesis of diethers in Archaea
Provedor de dados: 5
Autores: Gonthier, Isabelle; Rager, Marie-noëlle; Metzger, Pierre; Guezennec, Jean; Largeau, Claude.
The lipids of a deep-sea hydrothermal vent arch ae on, Thermococcus S 557, were isolated, purified and structurally determined. Based on acid methanolysis and spectroscopic studies, the polar lipids were shown to comprise diphytanyl glycerol and dibiphytanyl diglycerol, typical membrane lipids of Arch aea. From the neutral lipids, 2,3-di-O-dihydro-14,15-geranylgeranyl glycerol was isolated. This novel ether lipid is, very likely, a close intermediate in the biosynthesis of diphytanyl glycerol diether in Arch aea.
Tipo: Text
Palavras-chave: Mass spectrometry; NMR; Novel glycerol diether lipid; Thermococcus sp; Archaea.
Ano: 2001
URL: http://archimer.ifremer.fr/doc/2001/publication-736.pdf

Determining sedimentary material sources in a Brazilian urban lake using Zn stable isotope compositions of bottom sediments: a preliminary study
Provedor de dados: 5
Autores: Ferreira Araujo, Daniel; Boaventura, Geraldo; Mulholland, Daniel; Garnier, Jeremie; Machado, Wilson; Babinski, Marly; Dantas, Elton.
Zinc isotopes ratios provide qualitative and quantitative information about contributions from different Zn sources in the environment. Here we present the results of a preliminary study to identify the main sources controlling Zn isotope signatures of sediments from Lake Paranoá (Brazil), an urban water reservoir. The Zn isotope ratios were measured by MC-ICP-MS and expressed as δ66/64Zn notation relative to the JMC 3-0749-L solution. The studied sediments showed a narrow isotopic range (+0.00 to +0.13 ‰; n = 10) similar to that reported for intensely weathered soils worldwide (from -0.4 to +0.2‰). The geoaccumulation index (Igeo) also suggests low or negligible influence of Zn anthropogenic source. This research provides a baseline assessment of the Zn...
Tipo: Text
Palavras-chave: Stable isotopes; MC-ICP-MS; Mass spectrometry; Environmental pollution; Metal contamination; Weathering; Zinc isotopes.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00666/77763/79899.pdf

Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
Provedor de dados: 5
Autores: Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.
In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...
Tipo: Text
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf

Structure Elucidation, Relative LC–MS Response and In Vitro Toxicity of Azaspiracids 7–10 Isolated from Mussels (Mytilus edulis)
Provedor de dados: 5
Autores: Kilcoyne, Jane; Twiner, Michael J.; Mccarron, Pearse; Crain, Sheila; Giddings, Sabrina D.; Foley, Barry; Rise, Frode; Hess, Philipp; Willdns, Alistair L.; Miles, Christopher O.
Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7–10, 7–10, were isolated from shellfish and their structures, previously proposed on the basis of only LC–MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4–6, 4–6, and 7–10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC–MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a...
Tipo: Text
Palavras-chave: Azaspiracid; Structure confirmation; LC-MS molar response; NMR; Mass spectrometry; Purification; Jurkat T; Toxicity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/38059/36190.pdf

3-Acetoxy-fatty acid isoprenyl esters from androconia of the ithomiine butterfly Ithomia salapia
Provedor de dados: 5
Autores: Mann, Florian; Szczerbowski, Daiane; De Silva, Lisa; Mcclure, Melanie; Elias, Marianne; Schulz, Stefan.
Male ithomiine butterflies (Nymphalidae: Danainae) have hairpencils on the forewings (i.e. androconia) that disseminate semiochemicals during courtship. While most ithomiines are known to contain derivatives of pyrrolizidine alkaloids, dihydropyrrolizines or g-lactones in these androconia, here we report on a new class of fatty acid esters identified in two subspecies, Ithomia salapia aquinia and I. s. derasa. The major components were identified as isoprenyl (3-methyl-3-butenyl) (Z)-3-acetyloxy-11-octadecenoate, isoprenyl (Z)-3-acetyloxy-13-octadecenoate (12) and isoprenyl 3-acetyloxyoctadecanoate (11) by GC/MS- and GC/IR-analyses, microderivatizations, and synthesis of representative compounds. The absolute configuration of 12 was determined to be R. The...
Tipo: Text
Palavras-chave: Fatty acid esters; Mass spectrometry; Mimicry; Pheromones; Pyrrolizidine alkaloids.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00643/75512/76362.pdf

Enzymatic depolymerization of the GY785 exopolysaccharide produced by the deep-sea hydrothermal bacterium Alteromonas infernus : structural study and enzyme activity assessment
Provedor de dados: 5
Autores: Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine.
Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened...
Tipo: Text
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf

Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas
Provedor de dados: 5
Autores: Bernay, Benoît; Baudy-floc'H, Michele; Zanuttini, Bruno; Zatylny, Céline; Pouvreau, Stephane; Henry, Joël.
For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in...
Tipo: Text
Palavras-chave: Mass spectrometry; Myotropin; APGWamide; Egg laying; Molluscan.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1697.pdf

Requirements for screening and confirmatory methods for the detection and quantification of marine biotoxins in end-product and official control
Provedor de dados: 5
Autores: Hess, Philipp.
An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness...
Tipo: Text
Palavras-chave: Bioanalytical methods; Bioassays; Mass spectrometry; Liquid chromatography; Biological samples.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00006/11767/8989.pdf

Proteomic Analysis of the Chlorophyta Dunaliella New Strain AL-1 Revealed Global Changes of Metabolism during High Carotenoid Production
Provedor de dados: 5
Autores: Ben Amor, Faten; Elleuch, Fatma; Ben Hlima, Hajer; Garnier, Matthieu; Saint-jean, Bruno; Barkallah, Mohamed; Pichon, Chantal; Abdelkafi, Slim; Fendri, Imen.
The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel...
Tipo: Text
Palavras-chave: Proteome; Dunaliella; 18S rRNA gene; Carotenoids; Bidimensional gel electrophoresis; Mass spectrometry.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00402/51398/51973.pdf

Can the hemoglobin characteristics of vesicomyid clam species influence their distribution in deep-sea sulfide-rich sediments? A case study in the Angola Basin
Provedor de dados: 5
Autores: Decker, Carole; Zorn, N.; Le Bruchec, J.; Caprais, Jean-claude; Potier, N.; Leize-wagner, E.; Lallier, F. H.; Olu, Karine; Andersen, A. C.
Vesicomyids live in endosymbiosis with sulfur-oxidizing bacteria and therefore need hydrogen sulfide to survive. They can nevertheless live in a wide range of sulfide and oxygen levels and depths, which may explain the exceptional diversity of this clam family in deep-sea habitats. In the Gulf of Guinea, nine species of vesicomyid clams are known to live in cold-seep areas with pockmarks from 600 to 3200 m deep, as well as in the organic-rich sediments of the Congo deep-sea fan at 5000 m deep. Our previous study showed that two species living in a giant pockmark have different oxygen carriers, suggesting different adaptations to hypoxia. Here, we studied the hemoglobin structure and oxygen affinity in three other species, Calyptogena valdiviae, Elenaconcha...
Tipo: Text
Palavras-chave: Hemoglobin; Mass spectrometry; Oxygen affinity; Symbiont-bearing bivalve; Blood-clams; Cold seeps; Sulfide-rich sediments.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00358/46961/46875.pdf

Characterization of New Oligosaccharides Obtained by An Enzymatic Cleavage of the Exopolysaccharide Produced by the Deep-Sea Bacterium Alteromonas infernus Using its Cell Extract
Provedor de dados: 5
Autores: Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia.
Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own...
Tipo: Text
Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf

Effect of food supply on the detoxification in the blue mussel, Mytilus edulis, contaminated by diarrhetic shellfish toxins
Provedor de dados: 5
Autores: Marcaillou-le Baut, Claire; Haure, Joel; Mondeguer, Florence; Courcoux, Anne; Dupuy, Beatrice; Penisson, Christian.
The objective of this study was to identify a possible effect of food on the acceleration of decontamination in blue mussels, Mytilus edulis, containing diarrhetic toxins belonging to the okadaic acid (OA) structural group. An experimental protocol was designed to describe and compare the decontamination kinetics and detoxification rates of naturally OA-contaminated mussels that had either received or not received food for three weeks. The protocol was applied in two trials (in June 2006 and June 2007, called Ker06 and Ker07), conducted one year apart on samples of mussels collected in the same area, at the same season. Okadaic acid (OA), the main lipophilic toxin produced by the toxic alga Dinophysis acuminata, was analysed over the course of the...
Tipo: Text
Palavras-chave: Mussel; Feeding; Digestive gland; Depuration; Okadaic acid; Acyl esters; Marine lipophilic biotoxins; Mass spectrometry.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00025/13650/10883.pdf

UPLC-MSE Profiling of Phytoplankton Metabolites: Application to the Identification of Pigments and Structural Analysis of Metabolites in Porphyridium purpureum
Provedor de dados: 5
Autores: Juin, Camille; Bonnet, Antoine; Nicolau, Elodie; Berard, Jean-baptiste; Devillers, Romain; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
A fast and high-resolution UPLC-MSE analysis was used to identify phytoplankton pigments in an ethanol extract of Porphyridium purpureum (Pp) devoid of phycobiliproteins. In a first step, 22 standard pigments were analyzed by UPLC-MSE to build a database including retention time and accurate masses of parent and fragment ions. Using this database, seven pigments or derivatives previously reported in Pp were unequivocally identified: beta,beta-carotene, chlorophyll a, zeaxanthin, chlorophyllide a, pheophorbide a, pheophytin a, and cryptoxanthin. Minor amounts of Divinyl chlorophyll a, a chemotaxonomic pigment marker for prochlorophytes, were also unequivocally identified using the database. Additional analysis of ionization and fragmentation patterns...
Tipo: Text
Palavras-chave: Carotenoid; Chlorophyll; Dereplication; Divinyl chlorophyll a; Galactosyldiacylglycerol; Gracilamide; Mass spectrometry; MSE; Phytoplankton; Pigment; Porphyridium purpureum; UPLC.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00269/37994/36069.pdf

Structural data on a bacterial exopolysaccharide produced by a deep-sea Alteromonas macleodii strain
Provedor de dados: 5
Autores: Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire.
Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this...
Tipo: Text
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf

Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry
Provedor de dados: 80
Autores: Yu,Hai; Liu,Hui; Yan,Yizhong; Duan,Zhigui; Wang,Xianchun.
Background : Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spiderSelenocosmia huwena.Results : HWTX-IV was first lightly labeled with biotin under the optimized mild experimental conditions and the toxin labeled with a single biotin group (monobiotinylated HWTX-IV) was demonstrated by electrophysiological experiments to retain its...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biotin labeling; Huwentoxin-IV; Far-western blotting; Interacting protein; Mass spectrometry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200328

Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography
Provedor de dados: 80
Autores: Conceição,K; Bruni,FM; Sciani,JM; Konno,K; Melo,RL; Antoniazzi,MM; Jared,C; Lopes-Ferreira,M; Pimenta,DC.
Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrine-type serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was pre-fractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phyllomedusa nordestina; Bradykinin; Mass spectrometry; Natural peptides.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400004

Comparative analysis of the venom proteome of four important Malaysian snake species
Provedor de dados: 80
Autores: Vejayan,Jaya; Khoon,Too Lay; Ibrahim,Halijah.
BackgroundNaja kaouthia, Ophiophagus hannah, Bungarus fasciatusandCalloselasma rhodostomaare four venomous snakes indigenous to Malaysia. In the present study, their proteomic profile by two-dimensional gel electrophoresis (2-DE) have been separated and compared.ResultsThe 2-DE of venoms of the four species snake demonstrated complexity and obvious interspecies differences in proteome profiles. A total of 63 proteins were identified in the four species: C. rhodostoma -26, N. kaouthia -16, O. hannah -15 and B. fasciatus -6.ConclusionsDespite the identifications of major proteins in the four snake species, a large number of protein spots from the 2-DE were unidentified even though the spots displayed high-quality MALDI-TOF-MS spectra. Those identified...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; Snake venoms; Proteomics; Two-dimensional gel electrophoresis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200317

A new scenario of bioprospecting of Hymenoptera venoms through proteomic approach
Provedor de dados: 80
Autores: Santos,LD; Pieroni,M; Menegasso,ARS; Pinto,JRAS; Palma,MS.
Venoms represent a huge and essentially unexplored reservoir of bioactive components that may cure diseases that do not respond to currently available therapies. This review select advances reported in the literature from 2000 to the present about the new scenario of Hymenoptera venom composition. On account of new technologies in the proteomic approach, which presents high resolution and sensitivity, the combination of developments in new instruments, fragmentation methods, strategic analysis, and mass spectrometry have become indispensable tools for interrogation of protein expression, molecule interaction, and post- translational modifications. Thus, the biochemical characterization of Hymenoptera venom has become a major subject of research in the area...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hymenoptera; Venoms; Allergens; Phospholipase A1; Wasp venom antigen 5; Hyaluronoglucosaminidase; Mass spectrometry; Proteomics.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400003

Bufotenine is able to block rabies virus infection in BHK-21 cells
Provedor de dados: 80
Autores: Vigerelli,Hugo; Sciani,Juliana Mozer; Jared,Carlos; Antoniazzi,Marta Maria; Caporale,Graciane Maria Medeiros; Silva,Andréa de Cássia Rodrigues da; Pimenta,Daniel C.
Background Rabies is a fatal zoonotic neglected disease that occurs in more than 150 countries, and kills more than 55.000 people every year. It is caused by an enveloped single stranded RNA virus that affects the central nervous system, through an infection initiated by the muscular nicotinic acetylcholine receptor, according to many authors. Alkaloids, such as acetylcholine, are widespread molecules in nature. They are present in numerous biological fluids, including the skin secretion of many amphibians, in which they act (together with proteins, peptides and steroids) as protection agents against predators and/or microorganisms. Among those amphibians that are rich in alkaloids, there is the genus Rhinella.Methods Bufotenine was isolated from Rhinela...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Rhinella; Bufotenine; Alkaloids; Toxins; Mass spectrometry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200336

Proteomic characterization of the thermostable toxins from Naja naja venom
Provedor de dados: 80
Autores: Binh,DV; Thanh,TT; Chi,PV.
Naja naja snake venom presents abundant thermostable peptides. Many of them possess useful pharmacological activity that may be employed for drug development. For the proteomic characterization of such toxins, in the present study, Naja naja venom solution was heated up to 100°C for 10, 30, 60, 120, 180 and 300 minutes and protein fractions of non-heated and heated venom were analyzed by two-dimensional nano-liquid chromatography coupled online with tandem mass spectrometry. After heating for 300 minutes, a total of 32 peptides were still detected in the supernatant. The identified peptides belong to the following groups: cardiotoxins, neurotoxins and cytotoxins. It was found that thermostable peptides are able to preserve their analgesic activity after a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Naja naja; Snake venom proteome; Thermostable peptides; Mass spectrometry; Analgesic activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400014

Molecular and morphological characterization of hydrochar produced by microwave-assisted hydrothermal carbonization of cellulose
Provedor de dados: 86
Autores: Guiotoku,Marcela; Hansel,Fabricio Augusto; Novotny,Etelvino Henrique; Maia,Claudia Maria Branco de Freitas.
The objective of this work was to characterize the morphology and molecular composition of the hydrochar produced by microwave-assisted hydrothermal carbonization of cellulose. The produced hydrochar consists mainly of aggregate microspheres with about 2.0 µm in diameter, with aliphatic and aromatic structures and the presence of carbonyl functional groups. The aromatic groups are formed mainly by benzofuran-like structures, being chemically different from common cellulose char. Microwave-assisted hydrothermal carbonization yields a functionalized carbon-rich material similar to that produced by the conventional hydrothermal process.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anthropogenic dark earth of Amazonia; Biochar; Charcoal; Gas chromatography; Mass spectrometry; Nuclear magnetic resonance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500008

VINASSE EFFECT ON HERBICIDES CLOMAZONE AND TEBUTHIURON AVAILABILITY IN DIFFERENT KINDS OF SOILS
Provedor de dados: 87
Autores: MATOS,A.K.A.; CARBONARI,C.A.; VELINI,E.D.; GOMES,G.L.G.C.; TRINDADE,M.L.B.; MACEDO,G.C.
ABSTRACT The objective of this study was to evaluate the availability of herbicides clomazone and tebuthiuron in the solution in different kinds of soils saturated with water or vinasse. Samples of 30 soils with different characteristics were arranged on trays to the herbicides spraying. Then they were homogenized, placed in plastic cartridges and saturated with deionized water or vinasse, and remaining at rest during 18 hours. Two extractions were made, the first one quantified the presence of the herbicides in the soil solution and in the second one the total extraction of herbicide remaining in the soil was taken to determine the recovery percentage of each herbicide tested. For quantification, a LC-MS/MS system was used, a compound of a high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sugarcane; Mass spectrometry; HPLC; Sorption.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000400771

Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)
Provedor de dados: 92
Autores: Mendonça,Angélica; Bernardi Marchiotti,Rafaella C.; Firmino,Ellen L.B.; Santos,Pollyanna P.; Sguarizi Antonio,Denise; Serrão,José E.; Cardoso,Claudia A.L.; Antonialli Junior,William F.
ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mass spectrometry; MALDI-TOF/TOF; Proteins; Wasps.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262019000400322

Acylated anthocyanins from organic purple-fleshed sweet potato (Ipomoea batatas (L.) Lam) produced in Brazil
Provedor de dados: 63
Autores: Jesus,Monalisa Santana Coelho de; Santiago,Manuela Cristina Pessanha de Araújo; Pacheco,Sidney; Gouvêa,Ana Cristina Miranda Senna; Nascimento,Luzimar da Silva de Mattos do; Borguini,Renata Galhardo; Guerra,José Guilherme Marinho; Espindola,José Antonio Azevedo; Godoy,Ronoel Luiz de Oliveira.
ABSTRACT Acylated anthocyanins from a purple-fleshed sweet potato (PFSP), obtained by organic cultivation in Brazil, were characterized after separation by a high performance liquid chromatography-diode array detector (HPLC-PDA). These anthocyanins were manually collected at the detector output, concentrated and injected into a high resolution mass spectrometer (ESI-QTOF-MS2). Twenty-two acylated anthocyanins were detected. Among them, sixteen had been reported in the literature and six, derived from peonidin were reported for the first time in sweet potato roots in this study. These compounds showed molecular ions with accurate mass/charge ratios (m/z) of 909.2081, 961.3010, 961.2571, 963.3345, 1123.2932 and 1179.3862. Although anthocyanins in PFSP have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phenolic; Mass spectrometry; Natural dye; Antioxidant.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000400901

Sulfur utilization by rice and Crotalaria juncea from sulfate - 34S applied to the soil
Provedor de dados: 63
Autores: Trivelin,Paulo Cesar Ocheuze; Bendassolli,José Albertino; Muraoka,Takashi; Carneiro Jr.,Francisco.
In tropical soils with intensive agriculture an increasing sulfur deficiency has been verified in several crops. The low available S in these soils is caused by the continuous use of concentrated NPK fertilizers. The objective of this work was to evaluate the utilization by rice (Oriza sativa L.) and crotalaria juncea (Crotalaria juncea L.) of sulfur applied to the soil, under greenhouse conditions. Pots with 3 kg of an Argisol (Paleudalf) were used to test the isotopic technique with the stable isotope 34S, adding a solution of sodium sulfate labeled with 34S (14.30 ± 0.05 atom % of 34S) to the soil (70 mg SO4-S per kg-1 of soil) 18 days after sowing both species. The shoots of the crotalaria and rice were harvested, respectively on the 72nd and 122nd...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Stable isotope; 34S technique; Mass spectrometry; Sulfur uptake.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100030

Production of single superphosphate labeled with 34S
Provedor de dados: 63
Autores: Rossete,Alexssandra Luiza Rodrigues Molina; Carneiro,Josiane Meire Toloti; Bendassolli,José Albertino; Tavares,Claudineia Raquel Oliveira; Sant'Ana Filho,Carlos Roberto.
Single superphosphate is currently one of the mostly used fertilizers as an alternative source for phosphorus and sulphur. Sulphur presents four stable isotopes (32S, 33S, 34S, and 36S) with natural abundances of 95.00; 0.76; 4.22; and 0.014% in atoms, respectively. Single superphosphate labeled with the 34S isotope was obtained from a chemical reaction in stoichiometric amounts between Ca(H2PO4)2 and Ca34SO4.2H2O. Calcium sulphate (Ca34SO4.2H2O) was enriched with 5.85 ± 0.01 atoms % of 34S. The Ca(H2PO4)2 reagent was obtained from a reaction between CaCl2.2H2O and H3PO4. The reaction between the Ca(H2PO4)2 thus produced and the labeled Ca34SO4.2H2O compound was then performed to obtain the 34S-labeled single surperphosphate. The thermal decomposition of...
Palavras-chave: Isotopic determinations; Sulphur; Stable isotopes; Mass spectrometry; Labeled compounds.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000100013

การจำแนกเชื้อแบคทีเรีย Xanthomonas oryzae สาเหตุโรคข้าวโดยวิธีแมสสเปกโทรเมทรีของโปรตีนจากเซลล์แบคทีเรีย
Provedor de dados: 106
Autores: Prisana Wonglom; Vichai Kositratana; Sujin Phatarapoowadol.
100 isolates of phytopathogenic bacteria of rice, Xanthomonas oryzae, were identified by matrix-assisted laser desorption ionization–time of-flight mass spectrometry (MALDI-TOF MS). The results showed the whole-cell protein spectrum profile of mass spectra of Xanthomonas oryzae pv.oryzae,and X. oryzae pv. oryzicola shared the common spectra peak. However, X. oryzae pv.oryzae presented difference mass spectra at 2641.55, 3729.83, 4919.52, 5608.13, 9857.82, 12480.64 and 18737.92 m/z.whereas X. oryzae pv. oryzicola presented unique spectra of 6279.48 and 9179.89 m/z.
Tipo: Collection
Palavras-chave: Xanthomonas oryzae; Mass spectrometry; Bacteria of rice; MALDI-Biotyper; Phytopathogenic; Whole cell protein extracts; ข้าว; เชื้อแบคทีเรีย; การจำแนกเชื้อ; เชื้อสาเหตุ; โรคของข้าว; วิธีแมสสเปกโทรเมทรี.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5348

Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography
Provedor de dados: 52
Autores: Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos.
The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009

Proteomic analysis of whey from bovine colostrum and mature milk
Provedor de dados: 52
Autores: Golinelli,Luciana Pacheco; Conte-Junior,Carlos Adam; Paschoalin,Vânia Margaret Flosi; Silva,Joab Trajano.
The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine whey proteins; Colostrum proteins; Low-abundance proteins; Two-dimensional electrophoresis; Mass spectrometry; Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS).
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016

Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry and real-time PCR in a combined protocol for diagnosis of bloodstream infections: a turnaround time approach
Provedor de dados: 55
Autores: Quiles,Milene Gonçalves; Boettger,Bruno Cruz; Inoue,Fernanda Matsiko; Monteiro,Jussimara; Santos,Daniel Wagner; Ponzio,Vinicius; Carlesse,Fabianne; Cappellano,Paola; Carvalhaes,Cecilia Godoy; Pignatari,Antonio Carlos Campos.
ABSTRACT Bloodstream infections (BSIs) are serious infections associated with high rates of morbidity and mortality. Every hour delay in initiation of an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians to streamline therapy by exposing patients to broad-spectrum antimicrobials. Our objective was (1) to evaluate the accuracy and TAT of an optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and in-house real-time polymerase chain reaction (PCR) for bacterial identification and antimicrobial resistance profiling directly from positive blood bottles for diagnosing bloodstream infections...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bloodstream infections; Molecular diagnosis; Molecular panels; PCR; Mass spectrometry.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000300164

Proteomic data show an increase in autoantibodies and alpha-fetoprotein and a decrease in apolipoprotein A-II with time in sera from senescence-accelerated mice
Provedor de dados: 56
Autores: Guo,S.J.; Qi,C.H.; Zhou,W.X.; Zhang,Y.X.; Zhang,X.M.; Wang,J.; Wang,H.X.
We evaluated changes in levels by comparing serum proteins in senescence-accelerated mouse-prone 8 (SAMP8) mice at 2, 6, 12, and 15 months of age (SAMP8-2 m, -6 m, -12 m, -15 m) to age-matched SAM-resistant 1 (SAMR1) mice. Mice were sacrificed, and blood was analyzed by 2-dimensional electrophoresis combined with mass spectrometry. Five protein spots were present in all SAMP8 serum samples, but only appeared in SAMR1 samples at 15 months of age except for spot 3, which also showed a slight expression in SAMR1-12 m sera. Two proteins decreased in the sera from SAMP8-2 m, -6 m, and -12 m mice, and divided into 2 spots each in SAMP8-15 m sera. Thus, the total number of altered spots in SAMP8 sera was 7; of these, 4 were identified as Ig kappa chain V region...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SAMP8; Serum proteins; 2-DE; Mass spectrometry; M-T413.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500417

Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry
Provedor de dados: 58
Autores: Karcher,Daniel; Grenfell,Rafaella C.; Moreno,Andrea Micke; Moreno,Luisa Zanolli; Vasconcellos,Silvio Arruda; Heinemann,Marcos B.; Almeida Junior,Joao N. de; Juliano,Luiz; Juliano,Maria A.
ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospira; Brazil; Identification; Mass spectrometry; MALDI-TOF.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400900

Direct identification of bovine mastitis pathogens by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in pre-incubated milk
Provedor de dados: 58
Autores: Barreiro,Juliana R.; Gonçalves,Juliano L.; Grenfell,Rafaella; Leite,Renata F.; Juliano,Luiz; Santos,Marcos V.
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow. For direct identification method, quarter milk samples (n = 120) were skimmed by centrifugation (10,000 × g/10 min) and pre-incubated at 37 ºC for 12 h. After pre-incubation, quarter milk samples were submitted to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Milk; Bacteria; Mastitis; Mass spectrometry.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400801

A sensitive bioanalytical method development and validation of cabozantinib in human plasma by LC-ESI-MS/MS
Provedor de dados: 60
Autores: Inturi,Srikanth; Avula,Prameela Rani.
ABSTRACT A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of cabozantinib (CZ) in human plasma using cabozantinib-d4 (CZD4) as an internal standard (IS). Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 mm column with an isocratic mobile phase composed of 10mM Ammonium formate and Methanol in the ratio of (20:80 v/v), at a flow-rate of 0.7 mL/min. CZ and CZD4 were detected with proton adducts at m/z 502.2 ® 391.1 and 506.3 ® 391.2 in multiple reaction monitoring (MRM) positive mode respectively. Liquid-Liquid extraction method was used to extract the drug and IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cabozantinib/human plasma/validation; Mass spectrometry; Pharmacokinetic/study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200622

Evaluation of the bioequivalence of two formulations containing the combination of 400 mg of acetaminophen (paracetamol), 4 mg of phenylephrine and 4 mg of chlorpheniramine in capsules: open-label, three-way crossover study, partially replicated in healthy volunteers of both sexes
Provedor de dados: 60
Autores: Santos,Alessandra Ferreira dos; Santos,Quevellin Alves dos; Correa,Carlos Eduardo Melo; Coelho,Edvaldo Capobiango.
This study was carried out in order to compare the relative bioavailability of two different formulations containing 400 mg of acetaminophen + 4 mg of phenylephrine hydrochloride + 4 mg of chlorpheniramine maleate, Test formulation (Cimegripe®) and Reference formulation (Resfenol®) in 84 healthy volunteers of both sexes under fasting conditions. The study was conducted in a single dose, randomized, open-label, crossover 3-way and partially replicated. The tolerability was evaluated by the monitoring of adverse events and vital signs, results of clinical and laboratory tests. Plasma concentrations were quantified by validated bioanalytical methods using the ultra-performance liquid chromatography coupled to tandem mass spectrometry. The Cmax, Tmax, AUC0-t,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetaminophen; Phenylephrine; Chlorpheniramine; Bioequivalence; Partially replicated crossover; Ultra-high performance liquid chromatography; Mass spectrometry.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100564

Challenges in proteome analyses of tropical plants
Provedor de dados: 61
Autores: Balbuena,Tiago S.; Dias,Leonardo L. C.; Martins,Mariana L. B.; Chiquieri,Tatiana B.; Santa-Catarina,Claudete; Floh,Eny I. S.; Silveira,Vanildo.
Genome sequencing of various organisms allow global analysis of gene expression, providing numerous clues on the biological function and involvement in the biological processes studied. Proteomics is a branch of molecular biology and biotechnology that has undergone considerable development in the post-genomic era. Despite the recent significant advancements in proteomics techniques, still there is much to be improved. Due to peculiarities to the plant kingdom, proteomics approaches require adaptations, so as to improve efficiency and accuracy of results in plants. Data generated by proteomics can substantially contribute to the understanding and monitoring of plant physiological events and development of biotechnological strategies. Especially for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-DE; Mass spectrometry; Recalcitrant species; Proteomics; Unknown genome.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200001