Consulta: subjectFacets:"Methyl jasmonate"
Registros recuperados: 6
Data/hora: 06/06/2024 03:43:20

Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors
Provedor de dados: 69
Autores: Shohael,Abdullah Mohammad; Murthy,Hosakatte Niranjana; Hahn,Eun-Joo; Paek,Kee-Yoeup.
This study was concentrated on the production of eleutherosides and chlorogenic acid in embryogenic suspension cultures of Eleutherococcus senticosus by exposing them to different concentrations (50-400 µM) of methyl jasmonate (MJ) during the culture period. In the bioreactor cultures, eleutheroside content increased significantly by elicitation of MJ, however, the fresh weight, dry weight and growth ratio of embryos was strongly inhibited by increasing MJ concentrations. The highest total eleutheroside (7.3 fold increment) and chlorogenic acid (3.9 fold increment) yield was obtained with 200 µM MJ treatment. There was 1.4, 3.4 and 14.9 fold increase in the eleutheroside B, E, and E1 production respectively with such elicitation treatment. These results...
Tipo: Journal article
Palavras-chave: Eleutherosides; Elicitation; Methyl jasmonate; Secondary metabolite; Siberian ginseng; Suspension culture.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400016

Molecular cloning and expression profiling of a chalcone synthase gene from hairy root cultures of Scutellaria viscidula Bunge
Provedor de dados: 74
Autores: Lei,Wei; Tang,Shao-Hu; Luo,Ke-Ming; Sun,Min.
A cDNA encoding chalcone synthase (CHS), the key enzyme in flavonoid biosynthesis, was isolated from hairy root cultures of Scutellaria viscidula Bunge by rapid amplification of cDNA ends (RACE). The full-length cDNA of S. viscidula CHS, designated as Svchs (GenBank accession no. EU386767), was 1649 bp with a 1170 bp open reading frame (ORF) that corresponded to a deduced protein of 390 amino acid residues, a calculated molecular mass of 42.56 kDa and a theoretical isoelectric point (pI) of 5.79. Multiple sequence alignments showed that SvCHS shared high homology with CHS from other plants. Functional analysis in silico indicated that SvCHS was a hydrophilic protein most likely associated with intermediate metabolism. The active sites of the malonyl-CoA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chalcone synthase gene; Methyl jasmonate; Molecular cloning; Scutellaria viscidula Bunge.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200015

Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Provedor de dados: 47
Autores: Barradas-Dermitz,Dulce Ma.; Hayward-Jones,Patricia M.; Mata-Rosas,Martín; Palmeros-Sánchez,Beatriz; Platas-Barradas,Oscar B. J.; Velásquez-Toledo,Rodolfo F.
Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 &amp;#956;M). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth-associated metabolites; Methyl jasmonate; Paclitaxel precursor; Secondary metabolism; Suspension cell culture.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000100001

Azadirachtin biosynthesis induction in Azadirachta indica A. Juss cotyledonary calli with elicitor agents
Provedor de dados: 52
Autores: Rodrigues,Marcelo; Festucci-Buselli,Reginaldo Alves; Silva,Luzimar Campos; Otoni,Wagner Campos.
The use of cell and plant tissues culture techniques to produce economically important active metabolites has been growing. Among these substances, azadirachtin (AZA), produced by the neem tree (Azadirachta indica), has received considerable attention due to its bioinsecticide action. The main goal of this work was to analyze the AZA levels in neem cotyledonary calli. The calli were grown in agitated Woody Plant Medium (WPM) liquid medium, supplemented with glucose (Gl), hydrolyzed casein (HC) and methyl jasmonate (MeJ) as elicitor agent. An interaction was observed between these substances, depending on in vitro cultivation time with orbital agitation. The highest concentrations (average of 0.2470 µg g-1) of AZA were produced in the first and second weeks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neem; HPLC; Glucose; Hydrolysed casein; Methyl jasmonate.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200001

Effect of Methyl Jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves
Provedor de dados: 61
Autores: Soares,Alexandra Martins dos Santos; Souza,Thiago Freitas de; Jacinto,Tânia; Machado,Olga Lima Tavares.
Jasmonates are a class of plant hormones that mediate various aspects in gene and metabolic regulation, defense, stress responses, reproduction and, possibly, communication. Oxidative stress stimulates synthesis of antioxidant metabolites and enhances antioxidant enzyme activities that could protect plant tissues. The aim of this study was to verify the effects of methyl jasmonate (JAME) treatment on the reactive oxygen species (ROS) and on the activities of H2O2 scavenging enzymes, such as superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX EC; 1.11.1.1), and guaiacol peroxidase (GPX; EC 1.11.1.7) in Ricinus communis leaves. The activity of CAT and GPX was transient while SOD activity decreased and APX increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methyl jasmonate; Oxidative stress; Antioxidant enzymatic defenses; Catalase; Superoxide dismutase; Peroxidase; Ricinus comunnis; Signal transduction.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300001

Effect of methyl jasmonate treatments on the bioactive compounds and physicochemical quality of 'Fuji' apples
Provedor de dados: 118
Autores: Öztürk,Burhan; Altuntas,Ebubekir; Yildiz,Kenan; Ozkan,Yakup; Saracoglu,Onur.
Recently, plant growth regulators have been used for various purposes in apples. Methyl jasmonate (MeJA) has significant effects on the physiology, postharvest biology, processing, and chemical composition of apples. In the present study, the effects of MeJAMeJa treatments on the physicochemical quality and bioactive compounds (total antioxidant activity, total phenolics, and total anthocyanins) of 'Fuji' apples were investigated. The following doses of MeJa were used: 0 (control); 1; 120; 2,240; and 4,480 mg L-1. The highest geometric mean diameter, fruit mass, flesh firmness and skin thickness were obtained from the 2,240 mg L-1 MeJA treatment. Fruits harvested from control trees exhibited the highest total soluble solids content (TSSC) and pH, whereas...
Tipo: Journal article
Palavras-chave: Apple; Antioxidant; Color; Firmness; Methyl jasmonate; Phenolics.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000100018