Consulta: subjectFacets:"Methylation"
Registros recuperados: 24
Data/hora: 16/06/2024 00:14:37

Detection of differentially methylated regions of irradiated fig tree selections
Provedor de dados: 63
Autores: Rodrigues,Maria Gabriela Fontanetti; Soares,Murilo Racy; Gimenez,Daniele Fernanda Jovino; Fonseca,Larissa Fernanda Simielli; Torrieri,Erico; Ramos,Ester Silveira; Giuliatti,Silvana.
ABSTRACT Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, “Roxo-de-Valinhos”, which had previously used methylation-sensitive amplified polymorphism (MSAP) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ficus carica; DMRs; Methylation; Plant breeding; Epigenetics.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400285

In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer.
Provedor de dados: 119
Autores: PEREIRA, C. M.; CARVALHO, A. C. de; SILVA, F. R. da; MELENDEZ, M. E.; LESSA, R. C.; ANDRADE, V. C. C.; KOWALSKI, L. P.; VETTORE, A. L.; CARVALHO, A. L.
Abstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated...
Tipo: Artigo de periódico
Palavras-chave: Expressão gênica; ORESTES; Carcinogênese; Validação in vitro; Validação in silico; Open reading expressed sequence tags; Oral squamous cell carcinoma; Metilação; Gene expression; Methylation.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105692

Perfil epigenético e transcricional de fibroblastos isolados de pele bovina cultivados in vitro com substâncias desmetilantes de DNA.
Provedor de dados: 119
Autores: SCHUMANN, N. A. B.
Tese (Doutorado em Genética e Bioquímica) - Universidade Federal de Uberlândia, MG. Orientador: Maurício Machaim Franco, Embrapa Recursos Genéticos e Biotecnologia.
Tipo: Teses
Palavras-chave: TNCS; Epigenétic; SAH; Procaína; Expressão gênica; DNMT; TET; SCNT; Cloning; Clonagem; Metilação; DNA; Epigenetics; Methylation; Procaine; Gene expression.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113674

A cost-effective approach to DNA methylation detection by Methyl Sensitive DArT sequencing.
Provedor de dados: 119
Autores: PEREIRA, W. J.; PAPPAS, M. de C. R.; GRATTAPAGLIA, D.; PAPPAS JUNIOR, G. J.
Tipo: Artigo de periódico
Palavras-chave: DNA Sequencing; Epigenetic Inheritance; Genome; Transposons; Sulfites; Methylation; Genes; Genomics.
Ano: 2020
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124582

DNA methylation may affect beef tenderness through signal transduction in Bos indicus.
Provedor de dados: 119
Autores: SOUZA, M. M. de; NICIURA, S. C. M.; ROCHA, M. I. P.; PAN, Z.; ZHOU, H.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; AFONSO, J.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L.; KOLTES, J. E.; REGITANO, L. C. de A.
Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested...
Tipo: Artigo de periódico
Palavras-chave: Nelore; Epigenoma; Músculo de nelore; RRBS; GNAS; EBF3; Força de cisalhamento; Shear force; Epigenome; Muscle; Metilação; Bos Taurus; Bos Indicus; Músculo; Cattle; Methylation; DNA methylation.
Ano: 2022
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144

Epigenética: mecanismos, herança e implicações no melhoramento animal.
Provedor de dados: 119
Autores: PAIVA, J. T.; RESENDE, M. D. V. de; RESENDE, R. T.; OLIVEIRA, H. R.; SILVA, H. T.; CAETANO, G. C.; LOPES, P. S.; SILVA, F. F.
A epigenética é definida como mudanças na expressão gênica que podem ser herdadas e que não alteram a sequência do DNA. Existem dois mecanismos epigenéticos principais, a metilação do DNA e a modificação de histonas. Também se destaca o imprinting genômico, o qual está relacionado com a regulação gênica quando apenas um alelo (herdado do pai ou da mãe) se expressa. A herança epigenética transgeracional consiste da herança de marcas epigenéticas através de células germinativas, que controla os padrões de expressão gênica e são passados de uma geração para a outra. Dentro do programa de melhoramento animal é importante avaliar a extensão com que a herança epigenética transgeracional também afeta a eficiência da seleção. Uma melhor compreensão dos mecanismos...
Tipo: Artigo de periódico
Palavras-chave: Epigenoma; Expressão gênica; Histona; Transgeracional; Epigenome; Transgenerational; Metilação; Melhoramento Genético Animal; Gene expression; Methylation; Histones.
Ano: 2019
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110162

High frequency of p 16 promoter methylation in non-small cell lung carcinomas from Chile
Provedor de dados: 48
Autores: GUZMAN,LEDA M; KORIYAMA,CHIHAYA; AKIBA,SUMINORI; EIZURU,YOSHITO; CASTILLO,DARWINS; CORVALAN,ALEJANDRO; AGUAYO,FRANCISCO R.
The inactivation of tumour suppressor genes by aberrant methylation of promoter regions has been described as a frequent event in neoplasia development, including lung cancer. The p16 gene is a tumour suppressor gene involved in the regulation of cell cycle progression that has been reported to be inactivated by promoter methylation in lung carcinomas at variable frequencies around the world in a smoking habit dependent manner. The purpose of this study was to investigate the methylation status of the promoter region of the p16 gene in 74 non-small cell lung carcinomas from Chile. The frequency of p16 gene inactivation by promoter methylation was determined as 79.7% (59/74). When we considered histological type, we observed that p16 promoter methylation...
Tipo: Journal article
Palavras-chave: Lung carcinoma; Methylation; Promoter region.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400011

Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil
Provedor de dados: 48
Autores: Almeida,Fabiana Greyce Oliveira; Aquino,Priscila Ferreira de; Souza,Afonso Duarte Leäo de; Souza,Antonia Queiroz Lima de; Vinhote,Sonia do Carmo; Mac-Cormick,Thais Messias; Silva,Marcelo Soares da Mota; Chalub,Sidney Raimundo Silva; Fischer,Juliana de Saldanha da Gama; Carvalho,Paulo Costa; Carvalho,Maria da Gloria da Costa.
BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2...
Tipo: Journal article
Palavras-chave: Methylation; Colorectal cancer; Amazon; Epigenetic.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050

Assessment of changes in the brca2 and p53 genes in breast invasive ductal carcinoma in northeast Brazil
Provedor de dados: 48
Autores: Ramalho,Eduardo AVF; Silva-Filho,João LQ; Cartaxo,Marina FS; Cavalcanti,Carmelita BL; Rêgo,Moacyr JBM; Oliveira,Maria BM; Beltrão,Eduardo IC.
BACKGROUND: BRCA protein interacts with at least 13 different proteins that have been implicated with cancer susceptibility and loss of BRCA function is correlated to sensitivity to DNA crosslinking agents in preclinical models. RESULTS: BRCA2 methylation frequency was 44%, p53 Pro22 allele frequency was 32% and heterozygous frequency of Arg/Pro72 genotype was 60% which could be associated as risk factor for metastasis (p = 0.046 OR = 4.190). Regarding to polymorphism of codon 249 the frequency of Arg249 allele presented 82% which was considered not statistically significant. CONCLUSIONS: There was not statistical significance to BRCA2 promoter methylation with any parameters chosen. However, our findings suggest that patients who present heterozygous...
Tipo: Journal article
Palavras-chave: Breast cancer; Epigenetics; Metastasis; Methylation; Polymorphism.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100003

DNA methylation patterns of candidate genes regulated by thymine DNA glycosylase in patients with TP53 germline mutations
Provedor de dados: 56
Autores: Fortes,F.P.; Kuasne,H.; Marchi,F.A.; Miranda,P.M.; Rogatto,S.R.; Achatz,M.I.
Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant, hereditary cancer predisposition disorder. In Brazil, the p.R337H TP53 founder mutation causes the variant form of LFS, Li-Fraumeni-like syndrome. The occurrence of cancer and age of disease onset are known to vary, even in patients carrying the same mutation, and several mechanisms such as genetic and epigenetic alterations may be involved in this variability. However, the extent of involvement of such events has not been clarified. It is well established that p53 regulates several pathways, including the thymine DNA glycosylase (TDG) pathway, which regulates the DNA methylation of several genes. This study aimed to identify the DNA methylation pattern of genes potentially related to the TDG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Li-Fraumeni syndrome; TP53 gene; TDG; Methylation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700610

Placental hydroxymethylation vsmethylation at the imprinting control region 2 on chromosome 11p15.5
Provedor de dados: 56
Autores: Magalhães,H.R.; Leite,S.B.P.; Paz,C.C.P. de; Duarte,G.; Ramos,E.S.
In addition to methylated cytosines (5-mCs), hydroxymethylcytosines (5-hmCs) are present in CpG dinucleotide-enriched regions and some transcription regulator binding sites. Unlike methylation, hydroxymethylation does not result in silencing of gene expression, and the most commonly used methods to study methylation, such as techniques based on restriction enzymatic digestion and/or bisulfite modification, are unable to distinguish between them. Genomic imprinting is a process of gene regulation where only one member of an allelic pair is expressed depending on the parental origin. Chromosome 11p15.5 has an imprinting control region (ICR2) that includes a differentially methylated region (KvDMR1) that guarantees parent-specific gene expression. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methylation; Hydroxymethylation; Placenta; Genomic Imprinting; ICR2; KvDMR1.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100916

Role of microRNAs in the treatment of type 2 diabetes mellitus with Roux-en-Y gastric bypass
Provedor de dados: 56
Autores: Zhu,Z.; Yin,J.; Li,D.C.; Mao,Z.Q.
The aim of this study was to investigate the effect of Roux-en-Y gastric bypass (RYGB) on the peripheral blood microRNAs (miRNAs) of patients with type 2 diabetes mellitus (T2DM). miRNAs are small 20- to 22-nucleotide (nt) noncoding RNAs. They constitute a novel class of gene regulators that negatively regulate gene expression at the post-transcriptional level. miRNAs play an important role in several biological processes. Twelve patients with T2DM who were scheduled to undergo laparoscopic RYGB surgery were separated into two groups, using a body mass index of 30 kg/m2 as a cut-off point. Venous blood was collected before operation and 12 months after operation. A significant change was observed in the peripheral blood miRNA expression profile of both...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expression profile; Methylation; MicroRNA; Roux-en-Y gastric bypass; Type 2 diabetes mellitus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300705

DNA methylation regulates expression of VEGF-C, and S-adenosylmethionine is effective for VEGF-C methylation and for inhibiting cancer growth
Provedor de dados: 56
Autores: Da,M.X.; Zhang,Y.B.; Yao,J.B.; Duan,Y.X.
DNA hypomethylation may activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-adenosylmethionine (SAM) is a methyl donor in numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA. The main objectives of this study were to determine whether DNA hypomethylation correlated with vascular endothelial growth factor-C (VEGF-C) expression, and the effect of SAM on VEGF-C methylation and gastric cancer growth inhibition. VEGF-C expression was assayed by Western blotting and RT-qPCR in gastric cancer cells, and by immunohistochemistry in tumor xenografts. VEGF-C methylation was assayed by bisulfite DNA sequencing. The effect of SAM on cell apoptosis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: VEGF-C; Methylation; S-adenosylmethionine; Gastric cancer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201021

Effects of 5-aza-2′deoxycytidine on RECK gene expression and tumor invasion in salivary adenoid cystic carcinoma
Provedor de dados: 56
Autores: Zhou,X.Q.; Huang,S.Y.; Zhang,D.S.; Zhang,S.Z.; Li,W.G.; Chen,Z.W.; Wu,H.W.
Reversion-inducing cysteine-rich protein with kazal motifs (RECK), a novel tumor suppressor gene that negatively regulates matrix metalloproteinases (MMPs), is expressed in various normal human tissues but downregulated in several types of human tumors. The molecular mechanism for this downregulation and its biological significance in salivary adenoid cystic carcinoma (SACC) are unclear. In the present study, we investigated the effects of a DNA methyltransferase (DNMT) inhibitor, 5-aza-2′deoxycytidine (5-aza-dC), on the methylation status of the RECK gene and tumor invasion in SACC cell lines. Methylation-specific PCR (MSP), Western blot analysis, and quantitative real-time PCR were used to investigate the methylation status of the RECK gene and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RECK; Salivary adenoid cystic carcinoma; 5-aza-2′deoxycytidine; Methylation; Transwell assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300254

Clinicopathological significance of PTPN12 expression in human breast cancer
Provedor de dados: 56
Autores: Xunyi,Yuan; Zhentao,Yuan; Dandan,Jiang; Funian,Li.
Protein tyrosine phosphatase non-receptor type 12 (PTPN12) is a recently identified tumor suppressor gene (TSG) that is frequently compromised in human triple-negative breast cancer. In the present study, we investigated the expression of PTPN12 protein by patients with breast cancer in a Chinese population and the relationship between PTPN12 expression levels and patient clinicopathological features and prognosis. Additionally, we explored the underlying down-regulation mechanism from the perspective of an epigenetic alteration. We examined PTPN12 mRNA expression in five breast cancer cell lines using semi-quantitative reverse-transcription PCR, and detected PTPN12 protein expression using immunohistochemistry in 150 primary invasive breast cancer cases...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast cancer; Tumor suppressor gene; PTPN12; Prognosis; Methylation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200033

Methylation-specific PCR analysis in Col8A1 promoter in Creole cattle carrier of rob(1;29)
Provedor de dados: 69
Autores: Postiglioni,Alicia; García,Cristina B; Rincón,Gonzalo; Arruga,M. Victoria.
Robertsonian translocation (rob(1;29)) is the most frequent structural chromosomal abnormality in cattle. Heterozygous carriers have a normal phenotype but show a 3-5% decrease in fertility. Chromatin decondensation was evaluated similar to the inactive X chromosome when submitted to demethylating agent. Based on this result, and the concept that imprinted genes are essential in embryonic development, we decided to query genes located on BTA1 and BTA29 that could undergo genome imprinting. The collagen typeVIII-Î±1 (Col8A1) acted on extracellular matrix structural proteins. DNA bisulfite conversion and sequentiation methods were used to compare its differential methylation patterns. It was performed on eight Creole cattle DNA blood samples from normal and...
Tipo: Journal article
Palavras-chave: Bisulfite; Collagen; Creole cattle; Cytosines; Methylation.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300013

DNA demethylation during Chrysanthemum floral transition following short-day treatment
Provedor de dados: 69
Autores: Li,Zhongai; Li,Jie; Yanhua,Liu; Wang,Zicheng.
Background: Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum leaves during the floral transition induced by short-day (SD) treatment. Results: For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA...
Tipo: Journal article
Palavras-chave: Chrysanthemum; DNA; Methylation; Floral transition; Short day.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300009

Epigenetic alterations in human brain tumors in a Brazilian population
Provedor de dados: 74
Autores: Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda.
Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain tumors; Epigenetics; Methylation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001

Constitutional and somatic methylation status of DMRH19 and KvDMR in Wilms tumor patients
Provedor de dados: 74
Autores: Cardoso,Leila C.A.; Castaño,Jair A. Tenorio; Pereira,Hanna S.; Lima,Maria Angélica de F.D.; Santos,Anna Cláudia E. dos; Faria,Paulo S. de; Ferman,Sima; Seuánez,Héctor N.; Nevado,Julián B.; Almeida,José Carlos Cabral de; Lapunzina,Pablo; Vargas,Fernando R.
The most frequent epigenetic alterations in Wilms tumor (WT) occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19) that contains the IGF2 gene and an imprinted maternally expressed transcript (H19) and centromeric domain 2 (KvDMR) that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19) was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epigenetic; Histopathology; Methylation; MS-MLPA; Pyrosequencing.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500002

Otimização do Método Hartman e Lago de Preparação de Ésteres Metílicos de Ácidos Graxos.
Provedor de dados: 14
Autores: ANTONIASSI, R.; WILHELM, A. E.; MACHADO, A. F. de F.; GUEDES, A. M. M.; BIZZO, H. R.
A análise da composição em ácidos graxos é realizada, praticamente, para todos os produtos alimentícios, seja por questões nutricionais ou para atender a rotulagem nutricional. Em geral, é uma análise realizada por cromatografia gasosa que requer uma etapa prévia de conversão dos ácidos graxos em ésteres metílicos. Entre os métodos mais utilizados para esta conversão, destaca-se o método de metilação de Hartman e Lago, publicado em 1973. Trata-se do método mais utilizado no Brasil, pela sua simplicidade, rapidez e baixo custo. Neste estudo, foi realizada uma otimização do método original que permitiu aumentar o número de amostras analisadas com redução da quantidade de reagentes e do tempo de análise. As condições de análise foram avaliadas a fim de...
Tipo: Folhetos
Palavras-chave: Fats; Oil; Tecnologia de Alimento; Cromatografia Gasosa; Gordura; Metilação; Óleo; Gas chromatography; Food technology; Methylation.
Ano: 2018
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1105249

Parallel epigenetic modifications induced by hatchery rearing in a Pacific Salmon
Provedor de dados: 5
Autores: Le Luyer, Jeremy; Laporte, Martin; Beacham, Terry D.; Kaukinen, Karia H.; Withler, Ruth E.; Leong, Jong S.; Rondeau, Eric B.; Koop, Ben F.; Bernatchez, Louis.
A puzzling question in conservation biology is how to maintain overall fitness of individuals  bred in captive environment upon release into the wild, especially for rehabilitating declining or threatened species [1,2]. For salmonid species, a heritable change in fitness related traits and gene expression has been reported to occur in a single generation of captivity in hatchery environment [3–5]. Such rapid changes are congruent with models of  inadvertent domestication selection which may lead to maladaptation in the natural environment [4]. Arguably, the underlying mechanism by which captivity may induce  fitness difference between wild and captive congeners is still poorly understood. Short- term selection on complex phenotypic traits is expected to...
Tipo: Text
Palavras-chave: Epigenetics; Methylation; Coho salmon; Hatchery; RAD sequencing.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00409/52050/52722.pdf

A Michaelis–Menten type equation for describing methylmercury dependence on inorganic mercury in aquatic sediments
Provedor de dados: 5
Autores: Cossa, Daniel; Garnier, Cedric; Buscail, Roselyne; Elbaz-poulichet, Francoise; Mikac, Nevenka; Patel-sorrentino, Nathalie; Tessier, Erwan; Rigaud, Sylvain; Lenoble, Veronique; Gobeil, Charles.
Methylation of mercury (Hg) is the crucial process that controls Hg biomagnification along the aquatic food chains. Aquatic sediments are of particular interest because they constitute an essential reservoir where inorganic divalent Hg (HgII) is methylated. Methylmercury (MeHg) concentrations in sediments mainly result from the balance between methylation and demethylation reactions, two opposite natural processes primarily mediated by aquatic microorganisms. Thus, Hg availability and the activity of methylating microbial communities control the MeHg abundance in sediments. Consistently, some studies have reported a significant positive correlation between MeHg and HgII or total Hg (HgT), taken as a proxy for HgII, in aquatic sediments using...
Tipo: Text
Palavras-chave: Mercury; Methylmercury; Aquatic sediment; Methylation; Demethylation.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00171/28220/26520.pdf

Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): Comparison with salmonid species and analysis of CpG methylation
Provedor de dados: 5
Autores: Geay, Florian; Zambonino, Jose-luis; Reinhardt, Richard; Kuhl, Heiner; Santigosa, Ester; Cahu, Chantal; Mazurais, David.
Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was performed regarding the difference of the characteristics of a critical fragment of the fads2 gene promoter between sea bass and Atlantic salmon. The lower level of fads2 gene expression observed in sea bass suggested that fads2 gene putative promoter, which exhibited an...
Tipo: Text
Palavras-chave: FADS2; Sea bass; Gene expression; Promoter; Methylation.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00065/17621/15310.pdf

Study of Methylation Pattern of de Novo DNA Methyltransferase Genes and its Correlation with DNA Methylation Pattern of RUNX3 in Individuals with Gastric Cancer from Northern Region of Brazil
Provedor de dados: 77
Autores: Lima,Eleonidas Moura; Leal,Mariana Ferreira; Motta,Fábio José Nascimento; Assumpção,Paulo Pimentel de; Harada,Maria Lucia; Smith,Marilia de Arruda Cardoso; Burbano,RommelRodríguez; Casartelli,Cacilda.
Gastric cancer is the forth malignancy in frequency in the world. In the northern Brazil is the second neoplasia most frequent in males and the third most frequent in females. Genetic and epigenetic alterations are evolved on gastric carcinogenesis and DNA methylation is the epigenetic alteration better studied. We analyzed de novo DNA methyltransferases methylation pattern and its association with RUNX3 gene methylation pattern in Brazilian samples of intestinal-type and diffuse-type of gastric cancer. PCR methylation specific was used to evaluate DNA methylation pattern. Sixty-six samples were studied in this work. Only the gene RUNX3 presented altered methylation pattern, being methylated in 38.5% of gastric cancer intestinal-type samples and in 70% of...
Tipo: Journal article
Palavras-chave: Methylation; De novo DNA methyltransferases; RUNX3; Gastric cancer.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400022