Consulta: subjectFacets:"Methylmalonic acidemia"
Registros recuperados: 1
Data/hora: 01/06/2024 15:36:55

Chronic postnatal administration of methylmalonic acid provokes a decrease of myelin content and ganglioside N-acetylneuraminic acid concentration in cerebrum of young rats
Provedor de dados: 56
Autores: Brusque,A.M.; Rotta,L.; Pettenuzzo,L.F.; Junqueira,D.; Schwarzbold,C.V.; Wyse,A.T.; Wannmacher,C.M.D.; Dutra-Filho,C.S.; Wajner,M.
Levels of methylmalonic acid (MMA) comparable to those of human methylmalonic acidemia were achieved in blood (2-2.5 mmol/l) and brain (1.35 µmol/g) of rats by administering buffered MMA, pH 7.4, subcutaneously twice a day from the 5th to the 28th day of life. MMA doses ranged from 0.76 to 1.67 µmol/g as a function of animal age. Control rats were treated with saline in the same volumes. The animals were sacrificed by decapitation on the 28th day of age. Blood was taken and the brain was rapidly removed. Medulla, pons, the olfactory lobes and cerebellum were discarded and the rest of the brain ("cerebrum") was isolated. Body and "cerebrum" weight were measured, as well as the cholesterol and triglyceride concentrations in blood and the content of myelin,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmalonic acid; Glycerides; Lipids; Myelin; Rat brain; Methylmalonic acidemia.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200010