Consulta: subjectFacets:"Methyluric acids"
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Data/hora: 06/06/2024 15:06:13

Catabolism of caffeine and purification of a xanthine oxidade responsible for methyluric acids production in Pseudomonas putida L
Provedor de dados: 59
Autores: Yamaoka-Yano,Dirce Mithico; Mazzafera,Paulo.
Caffeine catabolism and a xanthine oxidase involved in the alkaloid breakdown were studied in Pseudomonas putida L, a strain displaying high ability to grow on this substrate. Cells cultured with unlabelled caffeine and 14C labeled caffeine and xanthine showed that this alkaloid was broken-down via theobromine/paraxanthine -&amp;gt; 7-methylxanthine -&amp;gt; xanthine -&amp;gt; uric acid -&amp;gt; allantoin -&amp;gt; allantoic acid. Methyluric acids were formed from the oxidation of theobromine, paraxanthine and 7-methylxanthine, although no bacterial growth was observed on these compounds, indicating that this might be due to a wide substrate specificity of xanthine oxidase. This was confirmed by activity staining in PAGE where activity was observed with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caffeine; Methylpurines; Methyluric acids; Pseudomonas; Xanthine oxidase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100013