Consulta: subjectFacets:"MiRNA"
Registros recuperados: 10
Data/hora: 05/06/2024 04:26:09

Construction and application of a built-in dual luciferase reporter for microRNA functional analysis
Provedor de dados: 69
Autores: Bi,Yanzhen; Zheng,Xinmin; Shao,Changwei; Pan,Wen; Jiang,Li; Ouyang,Huiwu.
Background: As key gene regulators, microRNAs post-transcriptionally modulate gene expression via binding to partially complementary sequence in the 3? UTR of target mRNA. An accurate, rapid and quantitative tool for sensing and validation of miRNA targets is of crucial significance to decipher the functional implications of miRNAs in cellular pathways. Results: Taking advantage of an improved restriction-free cloning method, we engineered a novel built-in dual luciferase reporter plasmid where Firefly and Renilla luciferase genes were assembled in a single plasmid named ?pFila?. This design eliminates the transfection of a separate control plasmid and thus minimizes the time and labor required for miRNA-target sensing assays. pFila consistently produces...
Tipo: Journal article
Palavras-chave: Biosensor; Luciferase; Ligase-independent; MiRNA; Target.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200011

An effective homologous cloning method for isolating novel miR172s from Phalaenopsis hybrida
Provedor de dados: 74
Autores: Han,Ying Ying; Yan,Qin Hua; Ming,Feng.
MiR172 is an important microRNA that regulates floral development in various plants and downregulates AP2 family members to relieve the stress on floral determinacy, leading to phase transition from vegetative to reproductive growth. In this work, PCR with primers designed based on the rice miR172 sequence was used to isolate two miR172-like transcripts from Phalaenopsis hybrida (PhmiR172-1 and PhmiR172-2) that were very similar to Oryza miR172d and Arabidopsis miR172b. RT-PCR indicated that the levels of these two transcripts were negatively correlated with the level of the Phalaenopsis AP2 (PhAP2) gene in stem, root, pedicel and sepal, and that both were co-expressed with PhAP2 in young buds. Overproduction of PhmiR172-2 in Arabidopsis led to early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homologous cloning; MiR172; MiRNA; Phalaenopsis; Sequence conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300014

Large miRNA survival analysis reveals a prognostic four-biomarker signature for triple negative breast cancer
Provedor de dados: 74
Autores: Andrade,Fernando; Nakata,Asuka; Gotoh,Noriko; Fujita,André.
Abstract Triple negative breast cancer (TNBC) is currently the only major breast tumor subtype without effective targeted therapy and, as a consequence, usually presents a poor outcome. Due to its more aggressive phenotype, there is an urgent clinical need to identify novel biomarkers that discriminate individuals with poor prognosis. We hypothesize that miRNAs can be used to this end because they are involved in the initiation and progression of tumors by altering the expression of their target genes. To identify a prognostic biomarker in TNBC, we analyzed the miRNA expression of a cohort composed of 185 patients diagnosed with TNBC using penalized Cox regression models. We identified a four-biomarker signature based on miR-221, miR-1305, miR-4708, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiRNA; MiR-221; MiR-1305; MiR-4708; RMDN2.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100110

microRNAs and the mammary gland: a new understanding of gene expression
Provedor de dados: 74
Autores: Gigli,Isabel; Maizon,Daniel Omar.
MicroRNAs (miRNAs) have been identified in cells as well as in exosomes in biological fluids such as milk. In mammary gland, most of the miRNAs studied have functions related to immunity and show alterations in their pattern of expression during lactation. In mastitis, the inflammatory response caused by Streptococcus uberis alters the expression of miRNAs that may regulate the innate immune system. These small RNAs are stable at room temperature and are resistant to repeated freeze/thaw cycles, acidic conditions and degradation by RNAse, making them resistant to industrial procedures. These properties mean that miRNAs could have multiple applications in veterinary medicine and biotechnology. Indeed, lactoglobulin-free milk has been produced in transgenic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lactation; Mastitis; Milk; MiRNA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400001

Cytosine methylation of mature microRNAs inhibits their functions and is associated with poor prognosis in glioblastoma multiforme
Provedor de dados: 5
Autores: Cheray, Mathilde; Etcheverry, Amandine; Jacques, Camille; Pacaud, Romain; Bougras-cartron, Gwenola; Aubry, Marc; Denoual, Florent; Peterlongo, Pierre; Nadaradjane, Arulraj; Briand, Josephine; Akcha, Farida; Heymann, Dominique; Vallette, François M.; Mosser, Jean; Ory, Benjamin; Cartron, Pierre-françois.
Background Literature reports that mature microRNA (miRNA) can be methylated at adenosine, guanosine and cytosine. However, the molecular mechanisms involved in cytosine methylation of miRNAs have not yet been fully elucidated. Here we investigated the biological role and underlying mechanism of cytosine methylation in miRNAs in glioblastoma multiforme (GBM). Methods RNA immunoprecipitation with the anti-5methylcytosine (5mC) antibody followed by Array, ELISA, dot blot, incorporation of a radio-labelled methyl group in miRNA, and miRNA bisulfite sequencing were perfomred to detect the cytosine methylation in mature miRNA. Cross-Linking immunoprecipiation qPCR, transfection with methylation/unmethylated mimic miRNA, luciferase promoter reporter plasmid,...
Tipo: Text
Palavras-chave: MiRNA; Cytosine-methylation; Epigenetics; DNMT3A; AGO4; Glioblastoma; Epitranscriptomics.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00610/72196/70980.pdf

Gene silencing: concepts, applications, and perspectives in woody plants
Provedor de dados: 63
Autores: Souza,Amancio José de; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanisms reveal similar biochemical pathways and appear to be related in several levels. The ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. This powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. This research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; MiRNA; SiRNA; Genetic transformation; Virus resistance.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600014

Alpinia oxyphylla Miq. extract changes miRNA expression profiles in db-/db- mouse kidney
Provedor de dados: 48
Autores: Du,Guankui; Xiao,Man; Zhang,Xuezi; Wen,Maoyu; Pang,Chi; Jiang,Shangfei; Sang,Shenggang; Xie,Yiqiang.
Abstract  Background A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-).  Results Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine. 17 and 13 miRNAs were statistically identified as differentially regulated in the DB/DB and db-/db- AOE mice, respectively, compared to the untreated db-/db- mice. Of...
Tipo: Journal article
Palavras-chave: MiRNA; Db-/db- mice; Alpinia oxyphylla Miq; Kidney; Diabetic nephropathy.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100206

The RNA interference revolution
Provedor de dados: 56
Autores: Lenz,G.
The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RNAi; SiRNA; MiRNA; Gene silencing; Knockdown.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200003

Voluntary exercise prior to traumatic brain injury alters miRNA expression in the injured mouse cerebral cortex
Provedor de dados: 56
Autores: Miao,W.; Bao,T.H.; Han,J.H.; Yin,M.; Yan,Y.; Wang,W.W.; Zhu,Y.H.
MicroRNAs (miRNAs) may be important mediators of the profound molecular and cellular changes that occur after traumatic brain injury (TBI). However, the changes and possible roles of miRNAs induced by voluntary exercise prior to TBI are still not known. In this report, the microarray method was used to demonstrate alterations in miRNA expression levels in the cerebral cortex of TBI mice that were pretrained on a running wheel (RW). Voluntary RW exercise prior to TBI: i) significantly decreased the mortality rate and improved the recovery of the righting reflex in TBI mice, and ii) differentially changed the levels of several miRNAs, upregulating some and downregulating others. Furthermore, we revealed global upregulation of miR-21, miR-92a, and miR-874 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Voluntary exercise; Running wheel; MiRNA; Traumatic brain injury.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500433

MicroRNA and cancer: a focus on mammary tumors in female dogs
Provedor de dados: 65
Autores: Salvador-Bernabé,Rosana Lino; Tinucci-Costa,Mirela; Amorim,Renee Laufer.
ABSTRACT:  Mammary tumors are the most frequent tumors reported in female dogs and have great relevance in veterinary oncology; however, little is known about the molecular mechanisms involved in the development of metastasis. An increasing number of human studies have suggested that epigenetic alterations, such as DNA methylation, miRNA, and histone modifications, are the predominant events leading to the metastatic phenotype in tumor cells and participate in regulating oncogenic signals associated with tumor spread. Among these epigenetic alterations, miRNAs have stood out in recent years, presenting a fundamental role in tumorigenesis. There are still few studies evaluating the role of miRNAs in canine mammary tissues. Thus, this paper aims to review...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oncology; Dog; Mammary carcinoma; MiRNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100600