Consulta: subjectFacets:"Mice"
Registros recuperados: 95
Data/hora: 07/06/2024 07:56:52

In vivo evaluation of the antimutagenic and antigenotoxic effects of β-glucan extracted from Saccharomyces cerevisiae in acute treatment with multiple doses
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; Salles,Maria José Sparça; Silva,Ariane Fernanda da; Kanno,Tatiane Yumi Nakamura; Lourenço,Ana Carolina dos Santos; Leite,Véssia da Silva; Matiazi,Hevenilton José; Pesarini,João Renato; Ribeiro,Lúcia Regina; Mantovani,Mário Sérgio.
Ample evidence suggests that cancer is triggered by mutagenic damage and diets or supplements capable of reducing such incidences can be related to the prevention of neoplasy development or to an improvement in life quality of patients who undergo chemotherapy. This research aimed to evaluate the antimutagenic and antigenotoxic activity of β-glucan. We set up 8 experimental groups: control (Group 1), cyclophosphamide (Group 2), Groups 3-5 to assess the effect of β-glucan administration, and Groups 6-8 to evaluate the association between cyclophosphamide and β-glucan. The intraperitonial concentrations of β-glucan used were 100, 150 and 200 mg/kg. Micronucleus and comet assays showed that within the first week of treatment β-glucan presented a damage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Β-glucan; Cyclophosphamide; Antimutagenicity; Antigenotoxicity; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300017

Genetic basis of the resistance to Strongyloides venezuelensis (Nematoda, Rhabdiasidae) infection in mice (Mus musculus)
Provedor de dados: 74
Autores: Marra,Nelson Mendes; Amarante,Alessandro F.T.; Amarante,Mônica R.V.
We investigated the resistance to Strongyloides venezuelensis primary infection of mice strains NIH (resistant) and C57BL/6 (susceptible) and the F1 and F2 offspring of crosses between these strains. The mice were infected with 2000 larvae and seven days later were sacrificed for parasite recovery and counting. There was no statistically significant (p &gt; 0.05) sex effect on resistance. The F1 mice showed an intermediate mean number of parasites as compared to the parental NIH and C57BL6 strains. Out of 400 F2 mice, the 10% most resistant mice were infected with 21 to 97 parasites, while the 10% most susceptible mice were infected with 1027 to 1433 parasites. We also found that F2 mice with black fur (n = 72), the same color as the C57BL/6 susceptible...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Strongyloides venezuelensis; Resistance; Genetics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100012

A critical evaluation of the effect of population size and phenotypic measurement on QTL detection and localization using a large F2 murine mapping population
Provedor de dados: 74
Autores: Li,Xinmin; Quigg,Richard J; Zhou,Jian; Xu,Shizhong; Masinde,Godfred; Mohan,Subburaman; Baylink,David J.
Population size and phenotypic measurement are two key factors determining the detection power of quantitative trait loci (QTL) mapping. We evaluated how these two controllable factors quantitatively affect the detection of QTL and their localization using a large F2 murine mapping population and found that three main points emerged from this study. One finding was that the sensitivity of QTL detection significantly decreased as the population size decreased. The decrease in the percentage logarithm of the odd score (LOD score, which is a statistical measure of the likelihood of two loci being lied near each other on a chromosome) can be estimated using the formula 1 - n/N, where n is the smaller and N the larger population size. This empirical formula has...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL mapping; Mice; Detection power; Population size; Phenotypic measurement.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100030

Alguns aspectos sobre roedores domésticos e seu controle em construções rurais.
Provedor de dados: 14
Autores: CAJUEIRO, I. V. de M.
bitstream/item/87587/1/CT04.pdf
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Roedor; Rato; Construção rural; Controle biológico; Controle físico; Controle químico; Rodents; Mice; Biological control; Physical control; Chemical control.
Ano: 1989
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/10073

Combined oral toxicity of azaspiracid-1 and yessotoxin in female NMRI mice
Provedor de dados: 5
Autores: Aasen, John A. B.; Espenes, Arild; Miles, Christopher O.; Samdal, Ingunn A.; Hess, Philipp; Aune, Tore.
For many years, the presence of yessotoxins (YTXs) in shellfish has contributed to the outcome of the traditional mouse bioassay and has on many occasions caused closure of shellfisheries. Since YTXs do not appear to cause diarrhoea in man and exert low oral toxicity in animal experiments, it has been suggested that they should be removed from regulation. Before doing so, it is important to determine whether the oral toxicity of YTXs is enhanced when present together with shellfish toxins known to cause damage to the gastrointestinal tract. Consequently, mice were given high doses of YTX, at 1 or 5 mg/kg body weight, either alone or together with azaspiracid-1 (AZA1) at 200 μg/kg. The latter has been shown to induce damage to the small intestine at this...
Tipo: Text
Palavras-chave: Azaspiracid-1; AZA1; Yessotoxin; YTX; Marine algal toxins; Absorption; Pathology; Sublethal; NMRI; Mice; LC-MS/MS; Oral toxicity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00034/14477/11776.pdf

Comparison of the Efficacy of Honey and Animal Oil in Accelerating Healing of Full Thickness Wound of mice skin
Provedor de dados: 77
Autores: Ghaderi,Reza; Afshar,Mohammad; Akhbarie,Hadi; Golalipour,Mohammad Jafar.
There are some medications and procedures that can be used to accelerate the healing of skin wounds. Some studies have demonstrated improvement of burn wound healing with honey treatment. In other hand, based on traditional medicine have improved wound healing with animal oil. This study was done to compare the efficacy of animal oil and honey in accelerating healing of full thickness wound of skin in mice. In this experimental study 36 male NMRI mice were subjected to full-thickness skin wounds under general anesthesia. Animals were randomly allocated to receive either single daily applications of placebo, animal oil and honey (n=12 for each group) respectively. On 4th, 7th and 10th days, four mice from each group were sacrificed using an overdose of...
Tipo: Journal article
Palavras-chave: Honey; Animal oil; Wound healing; Skin; Mice.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100027

Fetal Central Nervous System and Thymic Alterations Following Cyclophosphamide Treatment of Pregnant Mice
Provedor de dados: 77
Autores: Prakash,; Singh,Gajendra; Singh,Sukh Mahendra.
The present study assessed central nervous system (CNS) and immune system changes in murine fetuses after cyclophosphamide (CP) exposure during intrauterine life. A single CP dose of 0, 10, or 20mg/kg body weight was administered by intraperitoneal inj ection to pregnant mice (20/group) on day 11 of gestation (GD 11) and fetuses were evaluated on day 19 of gestation (GD 19). Fetuses were examined for external changes, and then the brains and thymuses were removed for further evaluations of histological changes, protein content, apoptotic cell count, DNA fragmentation, and in vitro cell proliferation using 1 fetus/litter for each assessment. Brains and thymuses from CP-exposed fetuses were smaller in size and distorted in overall shape compared to those...
Tipo: Journal article
Palavras-chave: Cyclophosphamide; Brain; Thymus; Mice; Teratology.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400016

Somatic, Biochemical and Hepatic Alterations in Wild Type Mice Chronically Fed High Fat Diet
Provedor de dados: 77
Autores: Neves,Renata Heisler; Barros Alencar,Alba Cristina Miranda de; Aguila,Marcia Barbosa; Mandarim-de-Lacerda,Carlos Alberto; Machado-Silva,José Roberto; Gomes,Delir Corrêa.
This study evaluated whether a high fat diet (HFC group) induces overweight, hepatic steatosis and plasma lipoproteins level alteration compared to standard chow diet (SC group). Female mice were submitted to each diet over 6 months. Body mass and food intake were evaluated weekly throughout the experiment. Total cholesterol, TG, LDL-c, HDL-c and VLDL-c were analyzed. Mice were sacrificed to remove liver, spleen, heart and intestine. The volume of the organs was determined according to the submersion method. Fixed livers were embedded in paraffin and stained with hematoxylin and eosin and Masson's trichrome. The analysis used a video microscope system and a test-system with 42 test-points. The volume density was estimated for hepatocytes, steatosis and...
Tipo: Journal article
Palavras-chave: Hypercholesterolemia; Experimental model; Mice; Cholesterol; Diet; Liver.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500018

Co-culture of Mouse Two-cell Embryos on Human Endometrial Stromal Cells in Proliferative and Secretory Phases
Provedor de dados: 77
Autores: Fakheri,Taravat; Nankali,Anisodowleh; Khazaei,Mozafar; Ghanbari,Ali; Abedini,Mehrandokht; Saeidiborojeni,Hamidreza.
There were no significant differences in the distribution of embryos reaching to 2- cells, 4- cells, morula or blastocysts culturing on human endometrial stromal cells (Secretory or proliferative phases). The percent of morula in stage A (without fragmentation), stage B (&lt;25% fragmentation), stage C (25-50% fragmentation) and stage D (&amp;gt;50% fragmentation) and did not showed significant differences between two coculture groups. Thus, the phase that the endometrial stromal cells were in thereby did not affect on the quality of embryos.
Tipo: Journal article
Palavras-chave: Co-culture; Embryo; Endometrium; Mice; Proliferative phase; Secretory phase.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400014

Morphometric Analysis of Mice Testicular Tubules after Administration of Malathion and Maca
Provedor de dados: 77
Autores: Bustos-Obregón,Eduardo; del Río,Fernando Costa; Sarabia,Luis.
Organophosphoric (OP) agropesticidas are amply used to increase food production. However, it has been verified that they induce alterations at testicular level related to the diminution of fertility in humans as in animals. On the other hand, different studies have been made to develop chemical or natural compounds that can induce an antagonistic effect to OP. In previous studies an extract from a plant, from the Peruvian Andes (Maca) has been recognized by its stimulating action on spermatogenesis. In the present study the effects of both external agents were evaluated on testicular sections of testis of adult male mice on a population of 52 mice CF1, divided at random in 4 groups (Control, Malathion, Maca, Malathion-Maca), with sacrifice intervals of...
Tipo: Journal article
Palavras-chave: Testis; Morphometry; Mice; Malathion; Maca.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200001

The Effects of Vasectomy on Epididymal Morphology and Sperm Parameters in Adult Male Balb/c mice
Provedor de dados: 77
Autores: Amini Mahabadi,Javad; Nikzad,Hossein; Taherian,Aliakbar; Mohammadi,Forouzan.
Vasectomy is a common contraceptive procedure in men. The present study is aimed to explore the impact of vasectomy on epididymal morphology and sperm parameters in adult male Balb/c mice. Twenty adult (age: 8 weeks) male Balb/c mice, weighting 20-30 g were used in the experiments. They were divided into 2 groups (vasectomy and sham). The operations were performed under sodium pentobarbital (40 mg/kg body weight, IP) anesthesia via a lower mid-abdominal incision. The left epididymis caput was fixed for histological studies and the right one was used for sperm count and motility. Progressive fast and slow sperm motility were significantly decreased in the vasectomized compared to the sham operation group (P&lt;0.05) and the number of immotile sperm in the...
Tipo: Journal article
Palavras-chave: Vasectomy; Epididymis; Mice; Sperm Motility.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400032

Protective Effect of Curcumin Against Nicotine-induced Damage on Reproductive Parameters in Male Mice
Provedor de dados: 77
Autores: Jalili,C; Khani,F; Salahshoor,M. R; Roshankhah,S.
Nicotine consumption can decrease fertility drive in males through inducing oxidative stress and DNA damage. The color of turmeric is because of a substance called curcumin for which some anti-oxidative and anti-inflammatory properties have been identified. In this study, various doses of curcumin (10, 30 and 60 mg/kg) and curcumin plus nicotine (10, 30 and 60 mg/kg) were administered intraperitoneally to male mice for 28 consequent days and reproductive parameters were determined. The results indicated that nicotine administration (0.5 mg/kg) significantly decreased testosterone level, count and motility of sperms, and testis weight compared to control group. However, increasing the dose of curcumin significantly increased reproductive indices in most of...
Tipo: Journal article
Palavras-chave: Curcumin; Nicotine; Reproductive Parameters; Mice.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000300017

Protective Effect of Tribulus terrestris Hydroalcoholic Extract against Cisplatin-Induced Cytotoxicity on Sperm Parameters in Male Mice
Provedor de dados: 77
Autores: Keshtmand,Zahra; Oryan,Shahrbanoo; Ghanbari,Ali; Khazaei,Mozafar.
Cisplatin is an anti-cancer drug used in chemotherapy. One of the limiting side effects of cisplatin is decreasing genital gland function, azoospermia and oligospermia. Tribulus terrestris has been used as an aphrodisiac. The present study amid to investigate protective effect of Tribulus terrestris hydroalcoholic extract against cisplatin-induced cytotoxicity on sperm parameters in mice. Male adult mice (n=30) were divided into control and 4 experimental groups (n=6). Control group received saline, first experimental group received cisplatin (5.5 mg/kg) and other three experimental groups received cisplatin (5.5 mg/kg) and different doses of hydroalcoholic extact of Tribulus terrestris (100, 300 and 500 mg/kg/i.p) respectively. On the day after the last...
Tipo: Journal article
Palavras-chave: Cisplatin; Tribulus terrestris; Sperm parameters; Mice.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000200029

Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice
Provedor de dados: 77
Autores: Asadi,Ebrahim; Shabani,Ronak; Ghafari,Soraya; Golalipour,Mohammad Jafar.
Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10...
Tipo: Journal article
Palavras-chave: Oocytes; Morphine sulfate; Vitamin E; Apoptosis; TUNEL assay; Mice.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029

The Effect of Rhododendron Honey on Mice Liver Tissue
Provedor de dados: 77
Autores: Kükner,Aysel; Ilter,Gizem; Söyler,Gizem; Rasgele,Pinar Göç; Kekeçoglu,Meral; Kambur,Merve.
Rhododendron honey, made by bees from rhododendron pollen, contains a toxic substance called grayanotoxin. Depending on the dose, the poisonous honey can result in serious effects such as cardiac arrhythmia, fibrillation, and myocardial infarction. The purpose of this study is to investigate the effects of the poisonous RH of the Black Sea Region on the liver. Male mice were divided into five groups of twelve mice each, two being the control groups (distilled water) and the others being the rhododendron honey (RH) groups (25, 50, and 75 mg/kg) and 0.01 mg/kg grayanotoxin (GTx) groups. Liver tissues were collected 24 and 48 h later. The sections were stained with hematoxylin, eosin and PAS, then the histopathological score was performed. Significant...
Tipo: Journal article
Palavras-chave: Rhododendron honey; Mice; Liver.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300004

Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
Provedor de dados: 77
Autores: Fernandes Boaro Martins,Márcia Regina; Carvalho Pinto e Silva,José Ricardo de.
The objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmium tetroxide. After dehydration, the sections were stained with uranyl acetate 2%. It was observed in early spermatids at Golgi phase, formation of acrosomal granule contained in the acrosomal vesicle, spheroidal nucleus with rugous reticulum around it, and Golgi apparatus made up of lamellae and vesicular structures. At cephalic hood phase, the granule suffers flattening and the marginal fossa is formed. In both phases, it was possible to observe the presence of...
Tipo: Journal article
Palavras-chave: Spermiogenesis; Spermatids; Mice.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400007

Study of Embryotoxicity of Mentha piperita L. During Organogenesis in Balb/c Mice
Provedor de dados: 77
Autores: Golalipour,Mohammad Jafar; Ghafari,Soraya; Maleki,Alireza; Kiani,Mossa; Asadi,Ebrahim; Farsi,Mirmerhdad.
Mentha piperita (Labiatae), commonly known as peppermint is a native Iranian herb which is used in folk medicine for various purposes. This study was carried out to reveal the teratogenic effect of Mentha piperita on mice fetuses. In this experimental study, pregnant Balb/c mice divided to four groups. Case group received 600 (treatment I) and 1200 (treatment II) mg/kg/day the hydroalcoholic extract of Mentha piperita during 6-15 of gestational days and one control group received normal saline during GD6-GD15 by gavages and other control group did not receive any matter during 6-15 of gestational days. Mice sacrificed at GD18 and embryos were collected. Macroscopic observation was done by stereomicroscope. 20 fetuses of each group were stained by Alizarin...
Tipo: Journal article
Palavras-chave: Mentha piperita; Teratogen; Bone ossification; Developmental toxicity; Mice.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300033

Ultrastructural Study of the Neuromuscular Junctions of Extensor Digitorum Longus Muscle (M. extensor digitorum longus) of the Mice
Provedor de dados: 77
Autores: Boaro,Sueli N; Fragoso Neto,Raul A.
The neuromuscular junction of the extensor digitorum longus muscle of fingers was analyzed in 21 young (three months) and old (from six to 25 months) mice, from both genders. Morphologic changes were found throughout the mouse life, being more frequent and visible with aging. According with the data described in the literature consulted and the observations taken in this research, it becomes clear that a continuous process of morphological remodeling occurs in all neuromuscular ultrastructural junctions of the extensor digitorum longus muscle of fingers, during the life of the animal. Theses changes are characterized by figures of myelin in the cytoplasm of Schwann cells, pleomorphic and multivesiclar bodies, mitochondrias with morphologically altered...
Tipo: Journal article
Palavras-chave: Neuromuscular junctions; Mice; Ultrastructure; Extensor digitorum longus muscle.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300045

Oxidative stress and comet assay in tissues of mice administered glyphosate and ampa in drinking water for 14 days
Provedor de dados: 46
Autores: Mañas,F; Peralta,L; Ugnia,L; Weyers,A; García Ovando,H; Gorla,N.
Excessive amounts of the herbicide glyphosate are incorporated daily to the soil and the ecosystems. AMPA is its major environmental breakdown product. In this study we determined the levels of thiobarbituric acid reactive substances (TBARs); quantified superoxide dismutase (SOD) and catalase (CAT) activity in liver, kidney, lung and heart, and performed the comet assay in blood and liver of mice administered glyphosate (40 or 400 mg/kg/day) or AMPA (100 mg/kg/day) in drinking water for 14 days. Exposure to glyphosate 400 mg/kg induced a statistically significant (p &lt;0.05) decrease of SOD activity in heart and an increase in CAT activity in kidney. In the comet assay there were statistically significant differences in all the treatments and tissues...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides; Mice; Genotoxicity.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300007

Cell-mediated immune response to Leishmania chagasi experimental infection of BALB/c immunosuppressed mice
Provedor de dados: 80
Autores: Machado,JG; Hoffmann,JL; Krause,VLK; da Silva,AV; Dias-Melicio,LA; Langoni,H.
Leishmaniasis, a zoonosis of worldwide distribution, presents a significant impact on immunosupressed patients. This study aimed to evaluate Leishmania chagasi infection in BALB/c mice immunosuppressed with dexamethasone. Spleen cells stimulated or not with L. chagasi were cultured for cytokine quantification (IFN-γ, IL-2, IL-4 and IL-10) by sandwich ELISA. Parasite loads in the spleen and liver were determined by means of culture microtitration. Immunosuppressed groups showed statistically lower spleen weight and CD4-cell percentage in blood on the day of infection and produced Th1 and Th2 cytokines on other days of the study. The other infected groups, weather immunosupressed or not, also produced Th1 and Th2 cytokines. Parasite loads in the spleen and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; Immunopathology; Mice; Interleukins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100013

Septic arthritis: immunopathogenesis, experimental models and therapy
Provedor de dados: 80
Autores: Colavite,Priscila Maria; Sartori,Alexandrina.
Septic arthritis is an inflammatory disease of the joints that is started by an infection whose most common agent is Staphylococcus aureus. In this review we discuss some of the most arthritogenic bacterial factors and the contribution of innate and specific immune mechanisms to joint destruction. Special emphasis is given to the induction of experimental arthritis by S. aureus in mice. The improvement of therapy by association of antibiotics with down-modulation of immunity is also included.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Septic arthritis; Staphylococcus aureus; Mice.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200205

Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
Provedor de dados: 80
Autores: Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004

DNA damage in BALB/c mice infected with Lacazia loboi and its relation to nutritional status
Provedor de dados: 80
Autores: Barbosa,Adriana Sierra Assencio Almeida; Oliveira,Larissa Ragozo Cardoso de; Tavares,Francilene Capel; Lima,Carlos Roberto Gonçalves de; Diório,Suzana Madeira; Calvi,Sueli Aparecida; Vilani-Moreno,Fátima Regina; Pereira,Paulo Câmara Marques.
AbstractBackground Jorge Lobo’s disease, also known as lacaziosis, is a cutaneous-subcutaneous mycosis with chronic evolution. It is caused by the fungus Lacazia loboi. Herein we report a study that relates the genotoxicity caused by L. loboi in isogenic mice with nutritional status, through a normal or restricted diet.Methods DNA damage was assessed in the peripheral blood by the comet assay (tail intensity).Results The results for leukocytes showed increases in the mean tail intensity in mice under dietary restriction, in infected mice under dietary restriction and in infected mice ingesting a normal diet.Conclusion These results indicate that dietary restriction and L. loboi infection may increase DNA damage levels in mice, as detected by the comet assay.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jorge Lobo’ disease; Lacazia loboi; Malnutrition; Mice; Genotoxicity; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100317

Tissue apoptosis in mice infected with Leptospira interrogans serovar Icterohaemorrhagiae
Provedor de dados: 80
Autores: Marinho,Márcia; Táparo,Cilene Vidovix; Oliveira Júnior,Itamar S.; Perri,Silvia Helena Venturoli; Cardoso,Tereza Cristina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Caspases; Programmed cell death; Mice; Inflammatory response.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100326

TNF-α and IL-6 immunohistochemistry in rat renal tissue experimentaly infected with Leptospira interrogans serovar Canicola
Provedor de dados: 80
Autores: Marinho,M; Monteiro,CMR; Peiró,JR; Machado,GF; Oliveira-Júnior,IS.
Leptospirosis is a public health problem worldwide and its etiology remains unclear. Its pathogenesis involves a complex interaction between host and infecting microorganism. The inflammatory reaction that controls the infection process also underscores many pathophysiological events occurring in leptospirosis. We investigated the presence of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in renal tissues by immunohistochemical and histopathological examination in animals experimentally inoculated with Leptospira serovar Canicola. All the tests were carried out 2, 7, 14, 21 or 28 days after inoculation. Although TNF-α and IL-6 had been detected in tissues throughout the observation period, these cytokines appeared more intensely during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Immunohistochemistry; IL-6; TNF-α; Mice.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300012

Freeze-drying Spermatozoa in Mice
Provedor de dados: 19
Autores: 鈴木, 宏志; 川瀬, 洋介.
Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried...
Palavras-chave: Mice; Freese-drying; Spermatozoa; マウス; 凍結乾燥; 精子.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4510

Comparison of PCR with Parasitology and Serology in the Diagnosis of a Low Virulent Strain of Trypanosoma brucei gambiense in mice
Provedor de dados: 19
Autores: Mbati, P. A.; Hirumi, K.; Inoue, Noboru; Situakibanza, N. H.; Hirumi, Hiroyuki; 井上, 昇.
Palavras-chave: Trypanosoma brucei gambiense; Mice; Mel-B; Diagnosis; PCR.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/310

Stimulatory factors for interleukin-12 production from murine splenic macrophages co-cultured with Babesia microti and Babesia rodhaini infected erythrocytes
Provedor de dados: 19
Autores: Hashiguchi-Kato, Rie; Thi, Pham Ngoc; Ohmori, Takashi; Tamahara, Satoshi; Katayama, Shinsuke; Shimada, Terumasa; Matsuki, Naoaki; Ono, Kenichiro.
Stimulatory factors for interleukin 12 (IL-12) production associated with Babesia microti and Babesia rodhaini infected erythrocytes were examined using an in vitro assay system established, since a remarkable increase of serum IL-12 concentration and differentiation of helper T cell (Th cell) into helper T cell type 1 (Th1 cell) was observed in early phase of infection with Babesia spp. in mice. To investigate direct stimulating activity of infected erythrocytes for IL-12 production, intact splenic macrophages were co-cultured with them and examined the expression of IL-12 mRNA by reverse transcription-polymerase chain reaction (RT-PCR). Both B. microti and B. rodhaini infected erythrocytes elicited IL-12 mRNA expression in co-cultured macrophages. Since...
Palavras-chave: Babesi microti and Babesia rodhaini infected erythrocyte; IL-12 Production; Mice; Splenic Macrophage; Stimulatory factor.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/151

Beneficial Effect of Hyperbaric Oxygen Therapy on the Follicular Survival in the Mouse Ovary after Transplantation
Provedor de dados: 19
Autores: HAYASHI, Satomi; ASANO, Tomoyoshi; KAKIZAKI, Ryujiro; SUZUKI, Hiroshi; 鈴木, 宏志.
A large proportion of follicles are lost during the initial ischemia that occurs after transplantation of ovarian tissues. Thus, the effect of hyperbaric oxygen therapy (HBO) on the follicular loss of ovarian tissues after transplantation was examined in mice. Ovarian slices from ICR mice were transplanted under the kidney capsule in ovariectomized ICR. Hyperbaric oxygen with 100% oxygen was initiated for 30 min at 2.5 atmospheres absolute immediately after transplantation, and this treatment was repeated at 48-h intervals for 2 weeks. The number of follicles was dramatically reduced at 2 weeks post transplantation. However, HBO was significantly effective in enhancing the survival of transplanted ovarian follicles. The survival rates of primordial and...
Palavras-chave: Hyperbaric oxygen; Mice; Ovary; Transplantation.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3857

Aggravation of pathogenesis mediated by aflatoxin B1 in mice infected with Trypanosoma brucei rhodesiense
Provedor de dados: 19
Autores: Kibugu, J.K; Makumi, J.N; Ngeranwa, J.J.N; Kagira, J.M; Gathumbi, J.K; Mwangi, J.N.
Aflatoxins are known to alter the pathogenesis of many infectious diseases, but such effects have not been evaluated in trypanosome infections. The aim of the present work was to assess the effects of aflatoxin B1 on the pathogenesis of Trypanosoma brucei rhodesiense infection using a murine model. Mice fed on 0.50 mg/kg aflatoxin b. wt. were infected with T. b. rhodesiense and compared to trypanosome infected and uninfected aflatoxin-fed controls. The clinical and pathological changes were determined and the quantitative data statistically analysed using standard methods. The results showed that infected aflatoxin-fed mice had pronounced dyspnoea, significantly (P&lt;0.05) reduced survival, extreme emaciation, pronounced macrocytic normochromic anaemia...
Palavras-chave: Aflatoxin B1; Trypanosomiasis; Pathogenesis; Mice.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2384

Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice
Provedor de dados: 19
Autores: LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; XUAN, Xuenan; IGARASHI, Ikuo; FUJISAKI, Kozo; SUGIMOTO, Chihiro; TOYODA, Yutaka; SUZUKI, Hiroshi; 玄, 学南; 五十嵐, 郁男; 鈴木, 宏志.
The mRNA expression patterns of EGF, HB-EGF, Amphiregulin, EGF receptor, IGF-1, CSF-1, IL-1 alpha, IL-1 beta, IL-1 receptor type 1, IL-1 receptor antagonist, LIF, COX-1, COX-2, Mucin-1, calcitonin, and rat USAG-1 mouse homologue, all of which are involved in the process of conceptus implantation to the endometrium, were examined during the estrous cycle by means of real-time quantitative PCR. COX-2, HB-EGF, LIF, Mucin-1, CSF-1, IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist were temporally regulated during the estrous cycle and highly expressed during the estrous stage. In the case of COX-1, EGF, IGF-1, and EGF receptor, the highest mRNA expression was during the diestrous stage. In contrast, the rat USAG-1 mouse homologue mRNA expression did not...
Palavras-chave: Estrous cycle; Implantation; Mice; Ovarian hormone.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3022

Protective immunity of blood plasma fraction containing Babesia rodhaini exoantigens in mice
Provedor de dados: 19
Autores: Kamiya, Shiho; Takabatake, Noriyuki; Fujii, Kei; Okamura, Masashi; Iseki, Hiroshi; Yokoyama, Naoaki; Igarashi, Ikuo.
Babesia spp. are major hemoprotozoan parasites in animals. Exoantigens, which are soluble antigens released by the parasites during their asexual development, have been successfully applied for the development of a vaccine against babesiosis. In this study, we examined the ability of exoantigens to induce protective immunity in mice against a rodent Babesia parasite, Babesia rodhaini. The BALB/c mice immunized with the blood plasma fraction of B. rodhaini-infected mice (B. rodhaini exoantigens) showed a significant decrease in the lethal rate when challenged with B. rodhaini. Western blot analysis with anti-B. rodhaini exoantigen immune serum specifically detected at least eleven proteins in the lysate of B. rodhaini-infected erythrocytes. Next, a cDNA...
Palavras-chave: Babesiosis; Babesia rodhaini; Exoantigen; Protective effect; Mice.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/149

Exposure of C57BL/6J Male Mice to an Electric Field Improves Copulation Rates with Superovulated Females
Provedor de dados: 19
Autores: HORI, Takuya; YAMSAARD, Thicomeporn; UETA, Yoshiko Yanagimoto; HARAKAWA, Shinji; KANEKO, Etsushi; MIYAMOTO, Akio; XUAN, Xuenan; TOYODA, Yutaka; SUZUKI, Hiroshi; 宮本, 明夫; 玄, 学南; 鈴木, 宏志.
It is well-known that there are considerable strain differences in the relative copulation rates between male and superovulated female mice. In particular, the C57BL/6J strain of mice has a lower rate of successful copulation. We examined the effect of exposure to an electric field on sexual behavior in C57BL/6J male mice. When C57BL/6J males were exposed to a 50 Hz, 45 kV/m electric field for 30 min per day for 11 days and placed in a cage with a superovulated female of the same strain, the successful copulation rates of males was significantly improved compared with unexposed males (P&lt;0.05). These results suggest that the exposure of C57BL/6J male mice to an electric field improves their sub-fertility activity in mating with superovulated females.
Palavras-chave: Mice; Male; Electric field; Copulation; Superovulation; Sexual behavior.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3021

Preliminary studies on the effects of orally-administered Transforming Growth Factor-beta on protozoan diseases in mice
Provedor de dados: 19
Autores: Namangala, Boniface; Inoue, Noboru; Sugimoto, Chihiro; 井上, 昇.
Transforming growth factor beta-1 (TGF-β1) is a pleiotropic cytokine with both pro- and antiinflammatory properties, depending on its environment and concentration. The present study evaluated the effects of orally-delivered TGF-β1 on mice parenterally-infected with various protozoan parasites. We report that while orally-administered TGF-β1 seems to confer partial protection against murine chronic babesiosis and acute trypanosomosis, no beneficial clinical effects were observed against acute babesiosis, malaria or toxoplasmosis. Taken together, these preliminary data suggest that the systemic effects conferred by exogenous TGF-β1 could be parasite speciesspecific. The variations in different parasitic infections could be due to (i) intrinsic differences...
Palavras-chave: Low-dose; Mice; Orally-delivered TGF-β1; Protection; Protozoan parasites.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2716

Effect of aflatoxin B1 on the therapeutic efficacy of suramin in Trypanosoma brucei rhodesiense-infected mice
Provedor de dados: 19
Autores: Kibugu, J.K.; Mdachi, R.E.; Kagira, J.M.; Muchiri, M.W.; Makumi, J.N.; Ngeranwa, J.J.N.; Auma, J. E.; Ngae, G.N.
Through immuno-suppression, aflatoxins could affect drug and vaccine efficacy. Such effects have not been evaluated in treatment of many diseases including trypanosomiasis. We assessed the effect of aflatoxin B1 on the efficacy of suramin, the drug used for treatment of early stage sleeping sickness, in a murine model. Mice were fed daily on a diet containing 0.50 mg aflatoxin/kg body weight or a placebo. They were infected with Trypanosoma brucei rhodesiense on day 7 post-aflatoxin exposure and then treated with one of 6 different doses of suramin (4.0, 4.5, 5.0, 5.5, 6.0 and 6.5 mg/kg body weight) at the onset of parasitemia. The mice were fed on aflatoxin diet for 30 days and the curative dose values (CD50, 75, and 90) computed and compared using a...
Palavras-chave: Aflatoxin B1; Trypanosomiasis; Suramin efficacy; Mice.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3130

Studies Related to Immunosuppression in Mice with Chronic Toxoplamosis
Provedor de dados: 19
Autores: Ito, Masanari; Igarashi, Ikuo; Fujita, Yukitoki; Omata, Yoshitaka; Saito, Atsushi; Nakagawa, Michio; Claveria, Florencia G.; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma; Cysts; Mice; Immunosuppression; Immunoregulator; Obiopeptide-1.
Ano: 1993
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/193

Expression of Amphiregulin During the Pre- and Post-implantation Period in the Mouse Reproductive Tract
Provedor de dados: 19
Autores: LEE, Dong-Soo; YANAGIMOTO UETA, Yoshiko; SUZUKI, Hiroshi; 鈴木, 宏志.
In mammals, embryo implantation is an essential step in reproduction. Implantation is a phenomenon that involves crosstalk between the blastocyst and the maternal endometrium. However, the molecular basis of the connections between the blastocyst and endometrium is not yet fully understood. Amphiregulin is a member of the epidermal growth factor family and is known to be expressed in the luminal epithelium of the mouse uterus on 3.5 days post coitum (dpc). Thus, to clarify the mechanism of amphiregulin at fetomaternal interface, we analyzed the expression pattern of amphiregulin mRNA in the oviducts and uteri of pregnant and psuedopregnant mice by means of real-time PCR. Amphiregulin expression in the pregnant uterus dramatically increased on 2.5 dpc,...
Palavras-chave: Mice; Implantation; Embryo transfer; Amphiregulin; MRNA.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3026

Establishment of a Pathogenicity Index for Mice to Pasteurella multocida Strains Isolated from Poultry and Swine
Provedor de dados: 89
Autores: Emery,BD; Furian,TQ; Chitolina,GZ; Pilatti,RM; Borges,KA; Salle,CTP; Moraes,HLS.
ABSTRACT Fowl cholera is a contagious disease that results from infection by the bacterium Pasteurella multocida. This microorganism is extensively distributed among animal species, but little is known regarding it’s pathogenesis and specificity to various hosts. Many studies using pathogenicity evaluation methods are subjective and difficult to quantify because they are often only involved in the observation of the lethal capacity of the agent in experimental inoculation. Due to a lack of more consistent data, this study aimed to establish a classification model of P. multocida pathogenicity in mice using strains isolated from poultry and swine. A total of 94 strains of P. multocida isolated from clinical cases of FC and from lungs of swine were tested. A...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Pasteurella multocida; Pathogenicity index.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200237

Effects of natural phenolic compounds from a desert dominant shrub Larrea divaricata Cav. on toxicity and survival in mice
Provedor de dados: 97
Autores: RÍOS,J.M.; MANGIONE,A.M.; GIANELLO,J.C.
It is known that generalist herbivores may circumvent intoxication by ingesting small quantities from a mixture of plant secondary metabolites. However a single chemical, a highly toxic one or the most abundant in the mixture could cause toxicity. Survivorship and toxicity in Rockland male mice were measured to determine if the toxic effects of the phenolic resin of creosote bush (Larrea divaricata Cav.) is due to its major constituent, the nordihydroguaiaretic acid (NDGA) or to the total concentration of phenolic compounds in this resin. This objective was accomplished by exposing mice to voluntary feeding on resin-treated rat chow and by oral gavaging of mice with the following doses and compounds: Resin-100 mg (n = 7), NDGA-15 mg (n = 8), and NDGA-100...
Tipo: Journal article
Palavras-chave: Larrea divaricata; Phenolic resin; NDGA; Survival; Mice.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000200011

Ex vivo study of hematopoiesis in protein malnutrition: characterization of bone marrow stromal cells in mice
Provedor de dados: 195
Autores: Santos, Ed Wilson; Dias, Carolina; Oliveira, Dalila Cunha de; Hastreiter, Araceli; Silva, Graziela Batista da; Beltran, Jackeline Soares de Oliveira; Paredes-Gamero, Edgar Julian; Borelli, Primavera.
Protein Malnutrition (PM) can promotes the modification of hematopoietic tissue, with changes of extracellular matrix components, affecting the evolution of the cell cycle of hemopoietic cells, reducing the production of precursors and hematopoietic cells both in erythroid series and in the number of leukocytes, leading to anemia and leukopenia. In view of these events, it was hypothetised that changes can occur in the formation and establishment of bone marrow stroma, as well as in cells proliferation, differentiation and maturation, resulting in the change in the microenvironment inducing hemopoese, affecting signaling cellular processes and the physiology of stem and progenitor cells
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hematopoiesis; Protein malnutrition; Bone marrow; Stromal cells; Mice hematopoiesis; Protein malnutrition; Bone marrow; Stromal cells; Mice.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31114

After restraint stress, mice with high and low immobility time have a different neurochemical and behavioral profile
Provedor de dados: 195
Autores: Reis-Silva, Thiago; Lima, Ana Paula; Moreira, Natalia; Calefi, Atilio; Sandini, Thaísa Meira; Florio, Jorge Camilo; Bernardi, Maria Martha.
High and low immobility on the tail suspension test is a common tool for assessing antidepressant drug effects. However, those parameters might be a natural phenotype present in the mice population and could be selected through behavior evaluation without an induced-drug behavior need. High immobility has been also related to depressed-like behavior, that in humans are characterized by wide range of symptoms, such as anhedonia, anxiety and tiredness. 
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stress; Mice; Behavior; Animal.
Ano: 2016
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/31107

Assessment of the cytotoxic, genotoxic, and antigenotoxic activities ofCeltis iguanaea (Jacq.) in mice
Provedor de dados: 42
Autores: BORGES,FLAVIO F.V.; MACHADO,THIAGO C.; CUNHA,KÊNYA S.; PEREIRA,KARLA C.; COSTA,ELSON A.; PAULA,JOSÉ R. DE; CHEN-CHEN,LEE.
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent (Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain, asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic. This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity, and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg−1). To evaluate its antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC, 4mg.kg−1). The frequencies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Genotoxicity; Mice; Medicinal plant.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300955

Amylin induces hypoglycemia in mice
Provedor de dados: 42
Autores: Guerreiro,Luiz H.; Silva,Daniel DA; Sola-Penna,Mauro; Mizurini,Daniella M.; Lima,Luís M.T.R.
Amylin is a 37-aminoacid pancreatic protein that exerts control over several metabolic events such as glycemia and lacticemia. Amylin has long been shown to induce increases in arterial plasma glucose. We decided to investigate whether amylin plays additional roles in the glucose metabolism. We evaluated glucose homeostasis using whole blood from the tail tip of fasting, conscious, unrestrained normal and streptozotocyn-induced diabetic mice following subcutaneous administration of mouse amylin. Subcutaneous injection of 1 μg mouse amylin caused a transient decrease in whole blood glucose in both normal and diabetic mice in the absence of insulin. The blood glucose levels were lowest approximately 2 hours after amylin administration, after that they...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylin; Islet associated polypeptide; Glycemia; Diabetes; Mice; Hypoglycemia.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000100349

Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice
Provedor de dados: 42
Autores: Silva,Jamile R.; Ferreira,Lício F.A.A.; Oliveira,Percíllia V.S.; Nunes,Ivanéia V.; Pereira,Ítalo S.; Timenetsky,Jorge; Marques,Lucas M.; Figueiredo,Tiana B.; Silva,Robson A.A.
Ureaplasma diversum is an opportunistic pathogen associated with uterine inflammation, impaired embryo implantation, infertility, abortions, premature birth of calves and neonatal pneumonia in cattle. It has been suggested that the intra-uterine infection by Ureaplasma diversum can cause vascular changes that hinder the success of pregnancy. Thus, the aim of this study was to evaluate the changes of intrauterine site of A/J mice in estrus or proestrus phase inoculated with Ureaplasma diversum. The infection was monitored at 24, 48 and 72 hours by the PCR methodology to detect the Ureaplasma in the inoculation site and the profile of circulating blood cells. Morphological changes, intensity of inflammation and the production of cytokines were compared. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Uterus; Ureaplasma; Mice.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200643

Effect of acetylsalicylic acid on total myenteric neurons in mice experimentally infected with Trypanosoma cruzi
Provedor de dados: 42
Autores: SOUZA,NOEMI D. DE; BELIN,BRUNA S.; MASSOCATTO,CRISTINA L.; ARAÚJO,SILVANA M. DE; SANT’ANA,DÉBORA M.G.; ARAÚJO,EDUARDO J.A.; P. FILHO,PHILENO; NIHEI,OSCAR K.; MOREIRA,NEIDE M.
Abstract: We investigated the effects of acetylsalicylic acid (ASA) on the total myenteric neuronal population in the descending colon in Trypanosoma cruzi-infected mice. Thirty-five male Swiss mice, 60 days old, were divided into a control group (C group), control group treated with ASA (CA group), infected group (I group), and infected group treated with ASA (IA group). A total of 1300 trypomastigotes of the Y strain of T. cruzi were intraperitoneally inoculated in the IA and I groups. The CA and IA groups were treated with ASA intraperitoneally. At 75 days post-infection (dpi), all of the animals were sacrificed. Neurons in the colon were stained with Giemsa, quantified, and measured. No difference in the course of infection was observed between the IA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic acid; Colon; Giemsa; Mice; Myenteric neurons; Trypanosoma cruzi.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300804

Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells
Provedor de dados: 42
Autores: Monteiro,Betânia S.; Argôlo-Neto,Napoleão M.; Nardi,Nance B.; Chagastelles,Pedro C.; Carvalho,Pablo H.; Bonfá,Laila P.; Filgueiras,Richard R.; Reis,Amanda S.; Del Carlo,Ricardo J.
Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp+ were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone therapy; Mesenchymal stem cell; Autologous graft; Mice; Calvaria.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300026

Protective Effect of Vitamin C on Triptolide-induced Acute Hepatotoxicity in Mice through mitigation of oxidative stress
Provedor de dados: 42
Autores: XU,PENGJUAN; LI,YOUYOU; YU,ZHICHAO; YANG,LIN; SHANG,RONG; YAN,ZIHANG.
Abstract: Triptolide, a purified diterpenoid from the herb Tripterygium wilfordii Hook.f., was widely used to treat many diseases. However, the hepatotoxicity of triptolide limited its clinical use. Research showed oxidative stress played an important role in triptolide-induced liver injury. To investigate the effect of vitamin C, which was one of the most effective antioxidants, on triptolide-induced hepatotoxicity and its potential mechanism in mice. In the present study, acute liver injury was induced by intraperitoneal injection of triptolide and vitamin C was orally administered. The results showed treatment with vitamin C prevented the triptolide-induced liver injury by reducing the levels of aspartate transaminase from 286.86 to 192.48 U/mL and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitamin C; Triptolide; Liver; Oxidative stress; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300713

Morphological alterations in salivary glands of mice (Mus musculus) submitted to tannin enriched diets: comparison with sialotrophic effects of sympathetic agonists stimulation
Provedor de dados: 45
Autores: Lamy,E.; Baptista,E.S.; Coelho,A.V.; Silva,F. Capela e.
In order to study the effects of tannins at histomorphological level, mice were either fed with three structurally different types of tannins (tannic acid, chestnut, and quebracho) or treated with isoproterenol, during 10 days. Acini of parotid and submandibular glands increased significantly, being the increase higher for parotid compared to submandibular glands, and higher in the quebracho compared with the other tannin groups. Sublingual acinar size also increased after tannin consumption, by opposition to isoproterenol-treated animals. The results present evidences that the effects produced by tannins are dependent on their structure.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Condensed tannin; Hydrolysable tannin; Isoproterenol; Salivary glands; Histology.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400012

Parameters for evaluation of clinical trial in mice infected with Trypanosoma cruzi
Provedor de dados: 45
Autores: Falkowski,G.J.S.; Sandri,P.F.; Tiyo,R.; Aleixo,D.L.; Araújo,S.M.
The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain - Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7th-11th and 15th-18th days of infection. There were significant differences (P&lt;0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P&amp;gt;0.05) between the groups in food consumption, exploration of self-cleaning and skin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clinical analysis; Chagas disease; Experimental infection; Mice; Trypanosoma cruzi.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600020

Bovine respiratory syncytial virus: immunohistochemichal detection in mouse and bovine tissues using a Mab against human respiratory syncytial virus
Provedor de dados: 45
Autores: Almeida,R.S.; Spilki,F.R.; Roehe,P.M.; Verinaud,L.M.C.; Arns,C.W.
An immunoistochemical (IHC) test was developed to detect bovine respiratory syncytial virus (BRSV) in cell cultures and tissues of experimentally infected mice and calves, using a commercial monoclonal antibody (Mab) against human respiratory syncytial virus (HRSV), as a less expensive alternative, instead of producing specific monoclonal antibodies to BRSV. Clinical samples from calves suffering respiratory disease were also submitted to this test. IHC detected BRSV antigens in mouse tracheas (3, 5 and 7 days post-infection) and lungs (5 and 7 days post-infection), and in one of three lungs from experimentally infected calves. Lungs samples from two naturally infected calves were tested and resulted positive for BRSV by the IHC test. These results suggest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine respiratory syncytial virus; Immunohistochemistry; Calf; Mice; Experimental infection; Monoclonal antibody.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000600001

Kint3-4 promotes apoptosis and inhibition of angiogenesis in solid Ehrlich Tumor
Provedor de dados: 45
Autores: Souza,C; Carvalho,L.F.; Ferreira,M.A.N.D; Pesquero,J.L.; Cassali,G.D.
A proteína Kint3-4 (PKint3-4), codificadora da angiostatina, é reconhecida por sua potencialidade antiangiogênica. O presente estudo teve como objetivo avaliar o efeito da proteína Kint3-4 no crescimento do tumor sólido de Ehrlich. Para isso, foram analisados a curva de desenvolvimento tumoral, o índice apoptótico e a dosagem de hemoglobina, a fim de se avaliar a angiogênese, em 20 camundongos Swiss fêmeas, inoculadas com o tumor sólido de Ehrlich em seus coxins plantares. Os resultados demonstraram a participação de PKint3-4 na indução à apoptose de células neoplásicas, na diminuição da concentração de hemoglobina e, principalmente, na diminuição do desenvolvimento tumoral. Sugere-se que a ação antitumoral, determinada pela sequência proteica utilizada,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Camundongo; Tumor de Ehrlich; Kint3-4; Apoptose; Angiogênese; Caspase-3; Tumor de Ehrlich; Mice; Ehrlich tumor; Kint3-4; Apoptosis; Angiogenesis; Caspase-3.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300036

Sanitary profile in mice and rat colonies in laboratory animal houses in Minas Gerais: I - Endo and ectoparasites
Provedor de dados: 45
Autores: Bicalho,K.A.; Araújo,F.T.M.; Rocha,R.S.; Carvalho,O.S.
The sanitary conditions of 13 animal houses in nine public institutions in Minas Gerais, and the presence of endo and ectoparasites of mice and rats colonies kept in these facilities were evaluated. Data about barriers to prevent the transmission of diseases and a program of sanitary monitoring were obtained through a questionnaire and local visit. Parasitological methods were performed for diagnosing mite, lice, helminthes, and protozoa parasites in 344 mice and 111 rats. Data have shown that the majority of the animal houses had neither proper physical environment nor protection barriers to prevent the transmission of infections. Parasitological results have shown that only one animal house (7.7%) had parasite free animals, whereas the others have...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Rat; Animal house; Parasite; Sanitary barrier.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600020

The use of mice as animal model for testing acute toxicity (LD-50) of toxic shock syndrome toxin
Provedor de dados: 45
Autores: Dias,R.S.; Carmo,L.S.; Heneine,L.G.D.; Rocha,P.H.; Barbosa,C.F.; Rodrigues,R.J.; Linardi,V.R.
Acute toxicity test (LD-50) using toxic shock syndrome toxin (TSST-1) was tested in BALB/c, C57BL/6 and Swiss mice. Animals (n = 10) were intraperitoneally injected with TSST-1 (0.01-10.0µg/mouse) followed 4h later by potentiating dose of lipopolysaccharide (75.0µg of LPS - E. coli O111:B4) and cumulative mortality was recorded over 72h. Control animals received either TSST-1 or LPS alone. The data were submitted to qui-Square test and acute toxicity test was calculated by probit analysis (confidence limits expressed as µg toxin/kg). BALB/c mice was the most sensitive (20.0µg/kg, 95% confidence limits: 9.0-92.0) followed by C57BL/6 (38.5µg/kg, 95% confidence limits: 9.11- 401.6). Data from Swiss mice was not conclusive, indicating only low sensitivity....
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mice; TSST-1; Acute toxicity (LD-50); Staphylococcus.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100024

Strongyloides venezuelensis infection susceptibility of seven inbred strains of mice
Provedor de dados: 45
Autores: Amarante,A.F.T.; Oliveira-Sequeira,T.C.G.
A trial was carried out to investigate the susceptibility of seven strains of mice to Strongyloides venezuelensis primary and secondary experimental infections, in order to provide the basis for genetic studies about resistance. Twelve six-week-old male inbred mice of the A/J, BALB/c, CBA/J, C3H/Hepos, C57BL/6, DBA/2 and NIH strains were infected s.c. with 2000 infective larvae. The mean worm counts (± SD) in the small intestine six days after infection were, in increasing order: 28 (± 19) in NIH; 647 (± 228) in BALB/c; 709 (± 425) in DBA/2; 731 (± 151) in C3H/Hepos, 801 (± 174) in CBA/J; 1024 (± 267) in C57BL/6 and 1313 (± 483) in A/J. C57BL/6 mice showed the highest fecal egg counts and NIH, the lowest. No eggs in fecal exams or nematodes in small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Strongyloides venezuelensis; Mice; Inbred; Resistance.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300009

Antioxidant capacity of vitamin C in mouse liver and kidney tissues
Provedor de dados: 47
Autores: Weyers,Alicia; Ugnia,Laura I.; García Ovando,Hugo; Gorla,Nora B.
In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice. The initial nmol of lipid hydroperoxides/g of tissue were 137 ± 11 in the kidney and 145 ± 15 in the liver, and the nmol of TBARs were 13 ± 0.7 and 12 ± 0.6, respectively. Pre-treatment with vitamin C reduced the levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipid peroxidation; Vitamin C; Ciprofloxacin; Mice.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100003

Extracellular breakdown of collagen by mice decidual cells. A cytochemical and ultrastructural study
Provedor de dados: 47
Autores: Godosevicius Katz,Sima.
The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy. Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles). Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals. Intracellular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Decidua; Acid phosphatase; Fasting; Electron microscopy; Mice.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300003

Role of berberine in ameliorating Schistosoma mansoni-induced hepatic injury in mice
Provedor de dados: 48
Autores: Dkhi,Mohamed A.
BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, &amp;#947;-glutamyl transferase in the liver homogenate, (iii) by...
Tipo: Journal article
Palavras-chave: Schistosoma mansoni; Berberine; Liver; Mice.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100008

Induced immune response of Escherichia coli BL21 expressing recombinant MSP1a and MSP1b proteins of Anaplasma marginale
Provedor de dados: 52
Autores: Tamekuni,Katia; Vidotto,Marilda Carlos; Felix,Samuel Rodrigues; Igarashi,Michelle; Garcia,João Luis; Coelho,Adriana Letícia; Gasparini,Marcela Ribeiro; Marana,Elizabete Regina Marangoni; Silva,Sérgio Silva da; Vidotto,Odilon.
This work aims to evaluate the potential of immunization with E. coli BL21 expressing the recombinant rMSP1a and rMSP1b proteins of Anaplasma marginale. E. coli BL21 was transformed with recombinant plasmids pET102/msp1α and pET101/msp1β, and rMSP1a and rMSP1b were expressed after induction by IPTG. BALB/c mice were vaccinated with formolized BL21/rMSP1a and BL21/rMSP1b, and the production in mice sera of whole IgG was determined by ELISA. The mice immunized with BL21/rMSP1a showed a better humoral response for whole IgG when compared to the mice immunized with BL21/rMSP1b; these mice exhibited a small response after the second vaccination. Sera of mice immunized with BL21/rMSP1a reacted via western blot with BL21 and rMSP1a, with molecular masses varying...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anaplasma marginale; MSP1a; MSp1b; Vaccine; Mice; Cattle.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700016

Methotrexate inhibits integrin adhesion molecules in the mouse model of pleurisy induced by carrageenan
Provedor de dados: 52
Autores: Dalmarco,Eduardo Monguilhott; Fröde,Tânia Silvia.
The aim of this work was to analyze the effect of methotrexate (MTX) upon leukocyte migration and expression of adhesion molecules CD11a/CD18 in the lung, 4 and 48 h after inflammation induction by carrageenan in mice. The results showed that MTX significantly decreased leukocyte influx and CD11a expression in the lung at 4 and 48 h of pleurisy (P &lt; 0.01). MTX also inhibited CD18 expression at 4 h but not 48 h of pleurisy (P &lt; 0.01). These results proved that MTX at the studied doses had important anti-inflammatory properties, acting primarily on leukocyte migration from the pleural cavity to the lung via inhibition of CD11a/CD18 expression in the mouse model of inflammation.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Methotrexate; CD11a/CD18 adhesion molecules; Leukocyte migration; Pleurisy; Mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500005

Brown Flaxseed Prevents DNA Damage Induced by 1,2-Dimethylhydrazine in a Pre-Clinical Model
Provedor de dados: 52
Autores: Pamplona-Silva,Maria Tereza; Morandi,Walter Vinicius; Bernardi,Luana; Tura,Bethânia Borges; Oliveira,Diego Duarte Marques de; Antoniolli-Silva,Andreia Conceição Milan Brochado; Mauro,Mariana de Oliveira; Oliveira,Rodrigo.
ABSTRACT The study evaluated the effects of brown flaxseed supplementation in natura on the prevention of DNA damage induced by 1,2-dimethylhydrazine (DMH) in vivo. The experimental groups were Negative and Positive Controls and the protocols of Pre-treatment, Simultaneous, Post-treatment, Pre+continuous in relation to the supplementation of brown flaxseed and administration with carcinogenic compound. The results showed that brown flaxseed supplementation does not cause genomic and genetic damage. In addition, brown flaxseed showed a chemopreventive food that reduced the damages assessed by the comet assay up to 94.07x and the damages assessed by the micronucleus assay up to 91.88x. Brown flaxseed supplementation also increased the frequency of monocytes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flaxseed; Antigenotoxicity; Chemoprevention; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100316

The combination of Passiflora alata and Valeriana officinalis on memory tasks in mice: comparison with diazepam
Provedor de dados: 52
Autores: Slomp Junior,Helvo; Seniski,Gerusa; Cunha,Cláudio da; Audi,Elizabeth Aparecida; Andreatini,Roberto.
The effect of a compound combining Valeriana officinalis and Passiflora alata extracts was tested on two mouse memory models: habituation and step-through inhibitory avoidance. Diazepam (1.0 and 2.5 mg/kg) was used as a positive control. Acute diazepam (2.5 mg/kg) before training impaired the habituation and performance in the inhibitory avoidance. On the other hand, acute phytotherapeutic compound (40-160 mg/kg), also before the training session, did not alter mouse behavior in these models. Repeated (15 days) treatment with the compound also did not impair the habituation. At the doses used, no locomotor effect was found. Taken together, the results suggest that, contrary to diazepam, the anxiolytic Valeriana officinalis and Passiflora alata compound did...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amnesic; Benzodiazepine; Memory; Mice; Phytotherapy.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600010

Induction of an antigen specific gut inflammatory reaction in mice and rats: a model for human Inflammatory Bowel Disease
Provedor de dados: 52
Autores: Teixeira,Gerlinde Agate Platais Brasil; Antunes,Danielle Mota Fontes; Castro Júnior,Archimedes Barbosa de; Costa,Janilda Pacheco da; Paschoal,Patrícia Olaya; Campos,Sylvia Maria Nicolau; Pedruzzi,Monique de Morais Bitetti; Araújo,Vinicius da Silva; Andrade,Luis Antônio Botelho; Moreno,Silvana Ramos Farias; Nobrega,Alberto Felix Antonio da; Cardoso,Gilberto Perez.
Food allergy is an adverse reaction that occurs in susceptible people when they eat sensitizing foods and is one of the causes of Inflammatory Bowel Disease (IBD). The effort to understand the induction process of these diseases is important as IBD is increasing worldwide, including in Brazil. The aim of this study was to develop an experimental antigen specific inflammatory process of the gut of mice and rats, using peanut seeds. Animals were immunized with peanut protein extract before their exposure to the in natura peanut seeds. Results showed that systemic immunization with peanut protein extracts rendered significantly higher antibody titers than control groups and that immunized animals submitted to a challenge diet containing peanuts presented time...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Food allergy; Peanuts; Inflammatory bowel disease; Rats; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300012

Immune response after rabies oral immunization in mice
Provedor de dados: 52
Autores: Silva,Camila Sloboda Pacheco da; Krüger,Ernesto Renato; Agottani,Jorge Victor Bacila; Oliveira,Rubens Chaguri de; Pereira,Sandro; Penha,Tânia Regina; Ollhoff,Rüdiger Daniel.
The objective of this study was to formulate an anti-rabies oral vaccine from the cell culture vaccine PV TECPAR to elicit the production of antibodies against the rabies in mice. A vaccine was developed using 10(7.5) DL50/0.03 ml viral antigens homogenised in lanovaseline to facilitate oral administration. Mice were vaccinated two times for seroconversion. Sera of the vaccinated mice showed a higher level of antibody production than the control group. These results could be used to direct the development of an anti-rabies oral vaccine.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Oral vaccine; Mice; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600006

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration
Provedor de dados: 52
Autores: Perini,João Angelo Lima; Stevanato,Flávia Braidotti; Visentainer,Jeane Eliete Laguila; Sargi,Sheisa Cyléia; Oliveira,Marcia Machado; Souza,Nilson Evelázio; Matsushita,Makoto; Visentainer,Jesuí Vergílio.
The objective of this study was to evaluate the incorporation of omega-3 fatty acids (n-3 FA) on male Swiss mice livers receiving diets based on linseed oil (LO) for up to 56 days. The mice were sacrificed at 7, 14, 28, 42, 56 days and FA concentration was analyzed by gas chromatography. A total of 13 FA were identified on LO feeds presenting alpha-linolenic acid (LNA) contents of 26.97 %. A total 22 FA were identified in the livers of the mice. Part of the LNA was converted into n-3 FA (20:3n-3, eicosapentaenoic acid-EPA, 22:5n-3, and docosahexaenoic acid-DHA), and some of the LNA was stored in the liver. LNA, EPA and DHA concentrations (mg/g total lipids ) from 0 days for up 56 days increased from 1.29 to 18.90 (LNA), 0.20 to 18.90 (EPA) and 41.26 to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polyunsaturated fatty acids; Mice; Liver; Linseed oil.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200012

Protective Effect of Rosa Canina Extract Against Doxorubicin-induced Testicular Toxicity in Mice
Provedor de dados: 52
Autores: Nowrouzi,Fatemeh; Azadbakht,Mehri; Kalehoei,Eshrat; Modarresi,Masoud.
Abstract The present study was aimed to investigate the in vivo effects of Rosa canina extract on doxorubicin-induced testicular toxicity in mice for the first time. Male NMRI mice were randomly divided into six treatment groups (10=per group) as follows: (I) vehicles, (II) doxorubicin alone (3 mg/kg, i.p. on days 7, 14 and 21), (III and IV): Rosa canina extract alone (100 mg/kg and 200 mg/kg per day, i.p. for 28 days), (V and VI) Rosa canina extract plus doxorubicin (each dose given 1 h post Rosa canina). Doxorubicin-treated mice displayed smaller body and testicular weights, decreased serum levels of testosterone, loss in the number of germ cells and Sertoli cells, and reduced sperm count, viability, morphology and motility. Doxorubicin treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rosa canina extract; Doxorubicin; Testes; Sperm; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100403

The Antinociceptive Effect of the Leaves and Flowers Ethanolic Extracts of Tabebuia aurea (Silva Manso) Benth &amp;amp; Hook. F. ex S. Moore
Provedor de dados: 52
Autores: Silva,Suellen Maria Albuquerque da; Silva Neto,Geraldo José da; Nascimento,Ivenis Raphael Cavalcante do; Viana,Max Denisson Maurício; Lima,Alyne Almeida de; Bezerra,Pedro Henrique Simões; Bastos,Maria Lysete de Assis; Moreira,Magna Suzana Alexandre; Campesatto,Eliane Aparecida.
ABSTRACT In view of the traditional use of Tabebuia aurea for treating pain and inflammation, the antinociceptive pharmacological potential of T. aurea ethanolic extracts (TAEE) was investigated through in vivo experimental models. First, the MTT assay was conducted to determine the potential cytotoxicity of the TAEEs. Afterwards, the acetic acid-induced writhing test and the formalin-, and glutamate-induced nociception tests were performed on Swiss adult mice treated with TAEEs (100 and 200 mg/kg doses, p.o.), or saline solution (control groups, 10mL/kg, p.o.), or standard drugs: dipyrone 40 mg/kg (p.o.), and morphine 5,7 mg/kg (i.p). In the MTT assay, none of the tested concentrations demonstrated signals of cytotoxicity. In the in vivo experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Analgesics; Nociception; Medicinal plants; Tabebuia aurea; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100443

Morphological aspects of the spermatic cord of mice (Mus musculus)
Provedor de dados: 62
Autores: Gonçalves,Andréia Maria Martarello; Borelli,Vicente.
A histological study of the morphological aspects of 33 spermatic cord pairs of adult mice (Mus musculus) showed that its their components were ensheated by a thin mesothelium capsule in 3 pairs. Under this capsule, completely surrounding the cord, there was a dense layer of adipose tissue. Its components were surrounded by loose connective tissue with a predominance of collagen fibers among reticular and elastic fibers. The testicular artery varied in diameter and was involved by the testicular veins, consisting of an inner layer formed by endothelium and an inner limiting elastic membrane, a middle layer formed by a thick smooth muscle layer supported by a network of reticular fibers, and an outer layer consisting of dense connective tissue close to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spermatic cord; Mice; Irrigation; Vascularization; Testis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000700009

Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly
Provedor de dados: 62
Autores: VISINTIN,José Antônio; GARCIA,José Fernando; PANTANO,Thais; D’ÁVILA ASSUMPÇÃO,Mayra Elena Ortiz.
Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whitten’s medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Embryo; Cryopreservation; Sucrose; Royal jelly.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400009

The protective effects of Baypamun®HK in mice experimentally infected with Toxoplasma gondii
Provedor de dados: 62
Autores: Cunha,Eva Laurice Pereira; Silva,Rodrigo Costa da; Da Silva,Aristeu Vieira; Langoni,Hélio.
The present work aimed to verify the effects of Baypamun®HK on the survival of albinic mice, experimentally infected with Toxoplasma gondii RH strain, treated or not with sulfadiazine-pirimetamine, as well as on the formation and the number of brain cysts. Four groups of 20 mices were inoculated with 10(5) tachizoites, subcutaneously, and undergone to different treatments. Serum tests were carried out by indirect immunofluorescence and samples of brain, liver and lung were collected from animals who died for cytological and brain cysts examinations. After 60 days, all survivors were sacrificed. Isolated Baypamun®HK didn't present a protective action against the infection by T. gondii, but its association sulfadiazine-pirimetamine, a specific treatment...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Baypamun®HK; Indirect immunofluorescence; Mice.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000300004

Genotoxic activity of the insecticide Nuvacron (Monocrotophos) detected by the micronucleus test in bone marrow erythrocytes of mice and in CHO cells
Provedor de dados: 54
Autores: Peitl Jr,Paulo; Sakamoto-Nojo,Elza T.; Cólus,Ilce M. de Syllos.
The organophosphorus insecticide Nuvacron (Monocrotophos) is a very toxic agent widely utilized in Brazilian agriculture. To evaluate the clastogenic potential of this insecticide, in vivo and in vitro micronucleus (MN) assay experiments were carried out on Swiss mice and on Chinese hamster ovary (CHO) cells, respectively. Nuvacron administered at doses of 2.5 and 5.0 mg/kg induced a statistically significant increase in the frequencies of MN detected in polychromatic bone marrow erythrocytes from animals (six/group) treated ip 24 h before. Exponentially growing CHAO cells were treated continuously (16h) with Nuvacron diluted in water to final concentrations of 1, 10, 100, 200, and 400 mug/ml. Three experiments were carried out using the cytokinesis-block...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxic; Insecticide Nuvacron; Micronucleus; Bone marrow; Erythrocytes; Mice; CHO cells.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400006

Inadequate timing between corticosteroid and antibiotics applications increases mortality due to sepsis
Provedor de dados: 55
Autores: Fadel,Marcus Vinícius Telles; Repka,João Carlos; Cunha,Cláudio Leinig Pereira da; Leão,Maria Terezinha C.
This study tested the hypothesis that the use of corticosteroids prior to antibiotics can lower the mortality rate in severe infections by S. aureus or Gram-negative bacilli, using an animal model. This study was a prospective and controlled study, placed in a university laboratory. Seven hundred and sixty mice distributed into three groups (Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae infected). The interventions in each group were: I) infection control (intra-peritoneal); II) treatment solely with antibiotics (teicoplanin or amikacin); III) antibiotics administered prior to the corticosteroid (methylprednisolone); IV) antibiotics administered after the corticosteroid. Mortality in the E. coli group, subgroup I: 100%; subgroup II: 55%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adrenal cortex hormones; Antibiotics; Sepsis; Survival; Mice; Animals; Laboratory; Severe infection; Peritonitis; Shock; Endotoxin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500013

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
Provedor de dados: 55
Autores: Leal,Elida A.; Moreira,Josimar D.; Nunes,Fernanda F.; Souza,Larissa R.; Martins,Janaina M.; Toledo,Vicente P.C.; Almeida,Alzira M.P.; Guimarães,Tania M.P.
ABSTRACT  Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response.  Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yersinia pestis; Antigens; Mice; Immune response; Vaccines.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice
Provedor de dados: 56
Autores: Fernandes,C.E.M.; Serafim,K.R.; Gianlorenço,A.C.L.; Mattioli,R.
Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis demonstrated that VUF-8430 induced a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex, suggesting that this dose impaired the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CREB; PCREB; VUF-8430; Western blotting; Emotional memory; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500603

Performance assessment of the single photon emission microscope: high spatial resolution SPECT imaging of small animal organs
Provedor de dados: 56
Autores: Mejia,J.; Reis,M.A.; Miranda,A.C.C.; Batista,I.R.; Barboza,M.R.F.; Shih,M.C.; Fu,G.; Chen,C.T.; Meng,L.J.; Bressan,R.A.; Amaro Jr,E.
The single photon emission microscope (SPEM) is an instrument developed to obtain high spatial resolution single photon emission computed tomography (SPECT) images of small structures inside the mouse brain. SPEM consists of two independent imaging devices, which combine a multipinhole collimator, a high-resolution, thallium-doped cesium iodide [CsI(Tl)] columnar scintillator, a demagnifying/intensifier tube, and an electron-multiplying charge-coupling device (CCD). Collimators have 300- and 450-µm diameter pinholes on tungsten slabs, in hexagonal arrays of 19 and 7 holes. Projection data are acquired in a photon-counting strategy, where CCD frames are stored at 50 frames per second, with a radius of rotation of 35 mm and magnification factor of one. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tomography; Preclinical imaging; Molecular imaging; Mice.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001100936

Combined effects of diethylpropion and alcohol on locomotor activity of mice: participation of the dopaminergic and opioid systems
Provedor de dados: 56
Autores: Gevaerd,M.S.; Sultowski,E.T.; Takahashi,R.N.
The widespread consumption of anorectics and combined anorectic + alcohol misuse are problems in Brazil. In order to better understand the interactive effects of ethanol (EtOH) and diethylpropion (DEP) we examined the locomotion-activating effects of these drugs given alone or in combination in mice. We also determined whether this response was affected by dopamine (DA) or opioid receptor antagonists. A total of 160 male Swiss mice weighing approximately 30 g were divided into groups of 8 animals per group. The animals were treated daily for 7 consecutive days with combined EtOH + DEP (1.2 g/kg and 5.0 mg/kg, ip), EtOH (1.2 g/kg, ip), DEP (5.0 mg/kg, ip) or the control solution coadministered with the DA antagonist haloperidol (HAL, 0.075 mg/kg, ip), the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diethylpropion; Ethanol; Locomotor activity; Dopamine; Opioid; Mice.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200015

Influences of alpha-tocopherol on cholesterol metabolism and fatty streak development in apolipoprotein E-deficient mice fed an atherogenic diet
Provedor de dados: 56
Autores: Peluzio,M.C.G.; Homem,A.P.P.; Cesar,G.C.; Azevedo,G.S.; Amorim,R.; Cara,D.C.; Saliba,H.; Vieira,E.C.; Arantes,R.E.; Alvarez-Leite,J.
Although the role of oxidized lipoproteins is well known in atherogenesis, the role of vitamin E supplementation is still controversial. There is also little information about cholesterol metabolism (hepatic concentration and fecal excretion) in the new models of atherosclerosis. In the present study, we evaluated the effect of moderate vitamin E supplementation on cholesterol metabolism and atherogenesis in apolipoprotein E (apo E)-deficient mice. Apo E-deficient mice were fed an atherogenic diet containing 40 or 400 mg/kg of alpha-tocopherol acetate for 6 weeks. Total cholesterol in serum and liver and 3-OH-alpha-sterols in feces, and fecal excretion of bile acids were determined and histological analyses of aortic lesion were performed. A vitamin E-rich...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidants; Atherosclerosis; Apo E; Mice; Alpha-tocopherol; Cholesterol.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200005

Hepatic changes in mice chronically infected with Helicobacter trogontum
Provedor de dados: 56
Autores: Moura,S.B.; Queiroz,D.M.M.; Rocha,G.A.; Comunian,L.B.; Cara,D.C.
We infected NIH germ-free female mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the lesions it induced in the liver of this host. Fifteen mice were challenged with a single dose of H. trogontum (test group) and killed 6, 12 and 18 months after inoculation (5 animals/group). Nine animals were challenged with 0.85% saline alone (control group) and killed at the same times. Fragments from the liver, cecum and colon were obtained for microbiologic and histologic examination. Stool samples were also collected. H. trogontum was detected in the cecum, colon and/or stool samples of all test mice. As expected, the bacterium was not isolated from any specimen obtained from the control animals. On the other...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Helicobacter; Helicobacter trogontum; Animal model; Liver; Mice.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900010

Contrasting effects of nitric oxide and corticotropin- releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice
Provedor de dados: 56
Autores: Miguel,T.T.; Gomes,K.S.; Nunes-de-Souza,R.L.
The anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFr1) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFr1 attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Corticotropin-releasing factor; Periaqueductal gray; Defensive behavior; Antinociception; Mice.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400003

Oral rapamycin attenuates atherosclerosis without affecting the arterial responsiveness of resistance vessels in apolipoprotein E-deficient mice
Provedor de dados: 56
Autores: Gadioli,A.L.N.; Nogueira,B.V.; Arruda,R.M.P.; Pereira,R.B.; Meyrelles,S.S.; Arruda,J.A.; Vasquez,E.C.
The objective of the present study was to assess the effects of the immunosuppressant rapamycin (Rapamune®, Sirolimus) on both resistance vessel responsiveness and atherosclerosis in apolipoprotein E-deficient 8-week-old male mice fed a normal rodent diet. Norepinephrine (NE)-induced vasoconstriction, acetylcholine (ACh)- and sodium nitroprusside (SNP)-induced vasorelaxation of isolated mesenteric bed, and atherosclerotic lesions were evaluated. After 12 weeks of orally administered rapamycin (5 mg·kg-1·day-1, N = 9) and compared with untreated (control, N = 9) animals, rapamycin treatment did not modify either NE-induced vasoconstriction (maximal response: 114 ± 4 vs 124 ± 10 mmHg, respectively) or ACh- (maximal response: 51 ± 8 vs 53 ± 5%, respectively)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rapamycin; Sirolimus; Atherosclerosis; Vascular responsiveness; Apolipoprotein E; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200012

Behavioral changes induced by cocaine in mice are modified by a hyperlipidic diet or recombinant leptin
Provedor de dados: 56
Autores: Erhardt,E.; Zibetti,L.C.E.; Godinho,J.M.; Bacchieri,B.; Barros,H.M.T.
The objective of the present study was to determine if the acute behavioral effects of cocaine acutely administered intraperitoneally (ip) at doses of 5, 10 and 20 mg/kg on white male CF1 mice, 90 days of age, would be influenced by leptin acutely administered ip (at doses of 5, 10 and 20 µg/kg) or by endogenous leptin production enhanced by a high-fat diet. The acute behavioral effects of cocaine were evaluated in open-field, elevated plus-maze and forced swimming tests. Results were compared between a group of 80 mice consuming a balanced diet and a high-fat diet, and a group of 80 mice fed a commercially available rodent chow formula (Ralston Purina) but receiving recombinant leptin (rLeptin) or saline ip. Both the high-fat-fed and rLeptin-treated mice...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mice; Cocaine; Leptin; Hyperlipidic diet; Interaction.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200014

Duration-controlled swimming exercise training induces cardiac hypertrophy in mice
Provedor de dados: 56
Autores: Evangelista,F.S.; Brum,P.C.; Krieger,J.E.
Exercise training associated with robust conditioning can be useful for the study of molecular mechanisms underlying exercise-induced cardiac hypertrophy. A swimming apparatus is described to control training regimens in terms of duration, load, and frequency of exercise. Mice were submitted to 60- vs 90-min session/day, once vs twice a day, with 2 or 4% of the weight of the mouse or no workload attached to the tail, for 4 vs 6 weeks of exercise training. Blood pressure was unchanged in all groups while resting heart rate decreased in the trained groups (8-18%). Skeletal muscle citrate synthase activity, measured spectrophotometrically, increased (45-58%) only as a result of duration and frequency-controlled exercise training, indicating that endurance...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Swimming; Cardiac hypertrophy; Mice; Myocardium.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200018

Alanyl-glutamine attenuates 5-fluorouracil-induced intestinal mucositis in apolipoprotein E-deficient mice
Provedor de dados: 56
Autores: Araújo,C.V.; Lazzarotto,C.R.; Aquino,C.C.; Figueiredo,I.L.; Costa,T.B.; de Oliveira Alves,L.A.; Ribeiro,R.A.; Bertolini,L.R.; Lima,A.A.M.; Brito,G.A.C.; Oriá,R.B.
Apolipoprotein E (APOE=gene, apoE=protein) is a known factor regulating the inflammatory response that may have regenerative effects during tissue recovery from injury. We investigated whether apoE deficiency reduces the healing effect of alanyl-glutamine (Ala-Gln) treatment, a recognized gut-trophic nutrient, during tissue recovery after 5-FU-induced intestinal mucositis. APOE-knockout (APOE-/-) and wild-type (APOE+/+) C57BL6J male and female mice (N=86) were given either Ala-Gln (100 mM) or phosphate buffered saline (PBS) by gavage 3 days before and 5 days after a 5-fluorouracil (5-FU) challenge (450 mg/kg, via intraperitoneal injection). Mouse body weight was monitored daily. The 5-FU cytotoxic effect was evaluated by leukometry. Intestinal villus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apolipoprotein E; 5-Fluorouracil; Alanyl-glutamine; Mucositis; Mice.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600493

Effects of exercise training on atrophy gene expression in skeletal muscle of mice with chronic allergic lung inflammation
Provedor de dados: 56
Autores: Durigan,J.L.Q.; Peviani,S.M.; Russo,T.L.; Silva,A.C.D.; Vieira,R.P.; Martins,M.A.; Carvalho,C.R.F.; Salvini,T.F.
We evaluated the effects of chronic allergic airway inflammation and of treadmill training (12 weeks) of low and moderate intensity on muscle fiber cross-sectional area and mRNA levels of atrogin-1 and MuRF1 in the mouse tibialis anterior muscle. Six 4-month-old male BALB/c mice (28.5 ± 0.8 g) per group were examined: 1) control, non-sensitized and non-trained (C); 2) ovalbumin sensitized (OA, 20 µg per mouse); 3) non-sensitized and trained at 50% maximum speed _ low intensity (PT50%); 4) non-sensitized and trained at 75% maximum speed _ moderate intensity (PT75%); 5) OA-sensitized and trained at 50% (OA+PT50%), 6) OA-sensitized and trained at 75% (OA+PT75%). There was no difference in muscle fiber cross-sectional area among groups and no difference in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; Muscle atrophy; Asthma; Gene expression; Mice.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000400005

Immunity and immunosuppression in experimental visceral leishmaniasis
Provedor de dados: 56
Autores: Goto,H.; Lindoso,J.A.L.
Leishmaniasis is a disease caused by protozoa of the genus Leishmania, and visceral leishmaniasis is a form in which the inner organs are affected. Since knowledge about immunity in experimental visceral leishmaniasis is poor, we present here a review on immunity and immunosuppression in experimental visceral leishmaniasis in mouse and hamster models. We show the complexity of the mechanisms involved and differences when compared with the cutaneous form of leishmaniasis. Resistance in visceral leishmaniasis involves both CD4+ and CD8+ T cells, and interleukin (IL)-2, interferon (IFN)- gamma, and IL-12, the latter in a mechanism independent of IFN- gamma and linked to transforming growth factor (TGF)-ß production. Susceptibility involves IL-10 but not IL-4,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Visceral leishmaniasis; Immunosuppression; T cell; Cytokines; Mice; Hamster.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400020

Acute buspirone abolishes the expression of behavioral dopaminergic supersensitivity in mice
Provedor de dados: 56
Autores: Queiroz,C.M.T.; Alcântara,F.B.; Yagüe,A.M.L.; Bibancos,T.; Frussa-Filho,R.
Previous studies have shown that rats withdrawn from long-term treatment with dopamine receptor blockers exhibit dopaminergic supersensitivity, which can be behaviorally evaluated by enhanced general activity observed in an open-field. Recently, it has been reported that co-treatment with the non-benzodiazepine anxiolytic buspirone attenuates the development of haloperidol-induced dopaminergic supersensitivity measured by open-field behavior of rats. The aims of the present study were: 1) to determine, as previously reported for rats, if mice withdrawn from long-term neuroleptic treatment would also develop dopaminergic supersensitivity using open-field behavior as an experimental paradigm, and 2) to examine if acute buspirone administration would...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Buspirone; Haloperidol; Dopaminergic supersensitivity; Tardive dyskinesia; Behavior; Mice.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200013

Leishmania (Leishmania) chagasi-infected mice as a model for the study of glomerular lesions in visceral leishmaniasis
Provedor de dados: 56
Autores: Prianti,M.G.; Yokoo,M.; Saldanha,L.C.B.; Costa,F.A.L.; Goto,H.
Renal involvement in visceral leishmaniasis (VL) is very frequent but the pathogenesis of this nephropathy is poorly understood. In previous studies using dogs with VL we have detected new immunopathological elements in the glomeruli such as T cells and adhesion molecules. Although Leishmania (Leishmania) chagasi-infected dogs and hamsters are considered to be good models for VL, their use is limited for immunopathologic studies. The use of isogenic mouse strains susceptible to L. (L.) chagasi infection was an alternative but, on the other hand, the renal lesions of these animals have not yet been characterized. Thus, our purpose in the present study was to characterize mice infected with L. (L.) chagasi as a suitable model to study VL nephropathy. Kidney...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Visceral leishmaniasis; Glomerulonephritis; Mice; Model; Leishmania (Leishmania) chagasi.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600011

Sex-dependent differences in the activities of acetylsalicylic acid-esterases in mouse kidneys
Provedor de dados: 56
Autores: Benedito,M.A.C.
Acetylsalicylic acid (ASA), the most used drug worldwide, is hydrolyzed to salicylic acid and acetate by esterases present in tissues of several species including humans. Sex differences in drug metabolism by rodent liver are documented in the literature. In this paper we report a difference in the activities of the esterases (ASA-esterase I and II) in the kidneys of male and female mice. In this species there is no difference between males and females in liver ASA-esterases (ASA-esterase I: males 38.5 ± 7.9 (N = 5) and females 31.6 ± 7.6 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P&amp;gt;0.05; ASA-esterase II: males 77.3 ± 17.4 (N = 5) and females 61.4 ± 15.1 (N = 5) nmol of salicylic acid formed min-1 mg protein-1, P&amp;gt;0.05)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acetylsalicylic acid; Esterases; Mice; Sex differences; Kidneys; Liver.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300004

Effects of nanoparticles of hydroxy-aluminum phthalocyanine on markers of liver injury and glucose metabolism in diabetic mice
Provedor de dados: 56
Autores: Melo,M.A.B.; Caetano,W.; Oliveira,E.L.; Barbosa,P.M.; Rando,A.L.B.; Pedrosa,M.M.D.; Godoi,V.A.F.
Photodynamic therapy, by reducing pain and inflammation and promoting the proliferation of healthy cells, can be used to treat recurrent lesions, such as diabetic foot ulcers. Studies using the photosensitizer phthalocyanine, together with the nanostructured copolymeric matrix of Pluronic® and Carbopol® for the treatment of diabetic foot ulcers and leishmaniosis lesions, are showing promising outcomes. Despite their topical or subcutaneous administration, these molecules are absorbed and their systemic effects are unknown. Therefore, we investigated the effect of the subcutaneous administration of the hydroxy-aluminum phthalocyanine hydrogel without illumination on systemic parameters, markers of liver injury, and liver energy metabolism in type 1 diabetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hydroxy-aluminum phthalocyanine; Hydrogel; Liver; Type 1 diabetes; Mice.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000100609

Survival and replication of Escherichia coli O157:H7 inside the mice peritoneal macrophages
Provedor de dados: 58
Autores: Al-Mariri,Ayman.
The replication of Escherichia coli O157:H7 on the resident peritoneal macrophages of four mice strains (BALB/c, CD1, C57BL, and Swiss) has been investigated. Macrophagial bactericidal killing activity was estimated via studying their ability to internalize (gentamicin-protected) E. coli during 2, 4, 24, and 48 h assays. Host genetic background has been found to show no significant effect on the ability of resident peritoneal macrophages to kill E. coli O157:H7.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: E. coli O157:H7; Macrophages; Mice; Gentamicin.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100002

Antagonistic activity of Lactobacillus acidophilus LA10 against Salmonella enterica serovar Enteritidis SE86 in mice
Provedor de dados: 58
Autores: Scapin,Diane; Grando,Williani Fabiola; Rossi,Eliandra Mirlei; Perez,Karla Joseane; Malheiros,Patrícia da Silva; Tondo,Eduardo Cesar.
Salmonella enterica serovar Enteritidis is one of the main pathogens responsible for foodborne illness in Brazil. Probiotic bacteria can play a role in defense and recovery from enteropathogenic -infections. In this study, the ability of Lactobacillus acidophilus LA10 to colonise and exert anta-gonistic effects in the gastrointestinal tract was tested before and during experimental infection in conventional mice contaminated with S. Enteritidis (SE86). A dose of 0.1 mL containing 10(8) viable cells of SE86 and L. acidophilus LA10 was orally administered by gavage to mice. The experiment was divided into groups. As a negative control, Group 1 was administered only sterile saline solution. As a positive control, Group 2 was administered only SE86. Group 3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S. Enteritidis SE86; Probiotics; Mice; Lactobacillus acidophilus LA10.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100008

Short-term high-fat diet affects macrophages inflammatory response, early signs of a long-term problem
Provedor de dados: 60
Autores: Santos,Ed Wilson; Oliveira,Dalila Cunha; Hastreiter,Araceli; Silva,Graziela Batista; Beltran,Jackeline Soares de Oliveira; Rogero,Marcelo Macedo; Fock,Ricardo Ambrósio; Borelli,Primavera.
Obesity is a chronic inflammatory disease that affects millions of people worldwide. Most studies observe the effects of a high-fat diet (HFD) in 10-12 weeks. This work investigated the effects induced by a HFD administered for 6 weeks on the nutritional status of mice and some aspects of the inflammatory response in mouse peritoneal macrophages. Male Swiss Webster mice, 2-3 months of age, were fed a control diet or HFD for 6 weeks. After this period, the mice were euthanized, and peritoneal macrophages were collected for immunoassays and assessment of biochemical parameters. A HFD was associated with increased cholesterol, insulin resistance, C-reactive protein (CRP), leptin, and serum resistin levels. Lipopolysaccharide (LPS)- stimulated adipocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High-fat diet/study; Mice; Macrophages; Metabolic syndrome; NF-kB.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100548

Hypoglycemic activity of Capparis ovata desf. var. palaestina zoh. methanol extract
Provedor de dados: 60
Autores: Okur,Mehmet Evren; Özbek,Hanefi; Polat,Derya Çiçek; Yılmaz,Sezen; Arslan,Rana.
Caper (Capparis ovata Desf. and Capparis spinosa L.) is naturally widespread in Turkey. Traditionally, buds, fruits, seeds and roots of this plant are used as tonic, diuretic, anti-rheumatic, expectorant, antidiabetic, and antifungal. The aim of this study is to evaluate potential hypoglycemic effect of C. ovata var. palaestina extracts in alloxan-induced diabetic mice. For this purpose; diabetic mice were administered with 100, 300, 500 mg/kg (i.p.) doses of methanol extract of bud and fruit. Blood glucose levels were screened 60, 120, 240 and 360 min. after treatment. Furthermore, high resolution mass spectrometry (HRMS) analysis, ABTS and DPPH free radical scavenging activity test, and phenolic and flavonoid compounds analysis of extracts were carried...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C. ovata var. palaestina/extract; C. ovata var; Palaestina/hypoglycemic effect/antioxidant effects; Diabetes mellitus; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300630

8-Propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one: A new coumarin with monoamine oxidase B inhibitory activity and possible anti-parkinsonian effects
Provedor de dados: 60
Autores: Olaya,María del Pilar; Vergel,Nadezdha Esperanza; López,Jose Luis; Viña,Dolores; Guerrero,Mario Francisco.
Parkinson’s disease is a common neurodegenerative disorder. In this study, the monoamine oxidase inhibitory activity and potential anti-parkinsonian effects of 8-propyl-6H-[1,3]dioxolo[4,5-g]chromen-6-one (FCS303), a new synthetic coumarin, were evaluated. To do this, we used the reserpine model of Parkinson’s disease, an assay of levodopa/carbidopa potentiation, the catalepsy model of haloperidol, and an in vitro assay against monoamine oxidase (MAO) activity. Additionally, lipid peroxidation and protein carbonyl group quantification was performed in mice brain homogenates previously treated with haloperidol. FCS303 inhibited monoamine oxidase B (MAO-B) with an IC50 of 5.46 ± 0.36 µM; however, there was no effect on monoamine oxidase A (MAO-A). The oral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parkinson’s disease; Monoamine oxidase B; Coumarin; Mice; Reserpine; Levodopa; Carbidopa.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100508

Antinociceptive activity of Ricinus communis seed’s hydroethanolic extract on male Balb/C mice
Provedor de dados: 65
Autores: Esfandyari,Zahra; Mirazi,Naser; Sarihi,Abdolrahman; Rafieian-Kopaei,Mahmoud.
ABSTRACT: Pain is a normal protective response to tissue injury caused by physical trauma, noxious chemicals and microbiological agents. Use of chemical drugs and medicinal plants is a conventional method to manage pain; however, their side effects have caused increased tendency to the use of herbal medicines among patients. This study was conducted to investigate antinociceptive action of Ricinus communis seed’s extract (RCE) in male Balb/C mice. In this experimental study, 72 male mice weighing 25-35gr were used. Animals were randomly divided into six groups of 12 mice each, including: Control group, three groups separately treated respectively with 100, 200, and 400mg/kg hydroethanolic R. communis seed extract, morphine (1mg/kg)-treated group, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antinociception; Ricinus communis; Morphine; Mice.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600601