Consulta: subjectFacets:"Microbial community"
Registros recuperados: 12
Data/hora: 06/06/2024 18:06:38

A microbial community analysis of the octocoral Eunicea fusca
Provedor de dados: 69
Autores: Duque-Alarcón,Angela; Santiago-Vázquez,Lory Z; Kerr,Russell G.
While there is a significant and growing body of knowledge describing the microbial communities of marine invertebrates such as sponges, there are very few such studies focused on octocorals. The octocoral Eunicea fusca is common on reefs in various regions of the Caribbean and has been the subject of natural product investigations. As part of an effort to describe the microbial community associated with octocorals, a culture-independent analysis of the bacterial community of E. fusca was conducted. Specifically, a 16S rDNA clone library analysis was performed to provide baseline data. A total of 40 bacteria members from 11 groups were found. In general, Proteobacteria were the dominant group with a total of 24 species and &amp;#945;-Proteobacteria...
Tipo: Journal article
Palavras-chave: 16S rDNA; Bacteria; Culture-independent analysis; Eunicea fusca; Microbial community; Octocoral.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500015

Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis
Provedor de dados: 69
Autores: Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu.
A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize...
Tipo: Journal article
Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007

Human-Driven Microbiological Contamination of Benthic and Hyporheic Sediments of an Intermittent Peri-Urban River Assessed from MST and 16S rRNA Genetic Structure Analyses
Provedor de dados: 5
Autores: Marti, Romain; Ribun, Sebastien; Aubin, Jean-baptiste; Colinon, Celine; Petit, Stephanie; Marjolet, Laurence; Gourmelon, Michele; Schmitt, Laurent; Breil, Pascal; Cottet, Marylise; Cournoyer, Benoit.
Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments....
Tipo: Text
Palavras-chave: Peri-urban river; Benthic and hyporheic sediments; Microbial community; High throughput sequencing (HTS); Fecal contamination.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00378/48886/49316.pdf

Cultivating the uncultured: limits, advances and future challenges
Provedor de dados: 5
Autores: Alain, Karine; Querellou, Joel.
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we...
Tipo: Text
Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf

Next-generation sequencing analysis reveals high bacterial diversity in wild venomous and non-venomous snakes from India
Provedor de dados: 80
Autores: Krishnankutty,Sajesh Puthenpurackal; Muraleedharan,Megha; Perumal,Rajadurai Chinnasamy; Michael,Saju; Benny,Jubina; Balan,Bipin; Kumar,Pramod; Manazhi,Jishnu; Kumar,Bangaruswamy Dhinoth; Santhosh,Sam; Thomas,George; Gupta,Ravi; Zachariah,Arun.
Abstract  Background: The oral cavities of snakes are replete with various types of bacterial flora. Culture-dependent studies suggest that some of the bacterial species are responsible for secondary bacterial infection associated with snakebite. A complete profile of the ophidian oral bacterial community has been unreported until now. Therefore, in the present study, we determined the complete bacterial compositions in the oral cavity of some snakes from India.  Methods: Total DNA was isolated from oral swabs collected from three wild snake species (Indian Cobra, King Cobra and Indian Python). Next, the DNA was subjected to PCR amplification of microbial 16S rRNA gene using V3-region-specific primers. The amplicons were used for preparation of DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial community; Next-generation sequencing; Venomous snake; Hypervariable region.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100331

Utilization of rice byproducts as carbon sources in high-density culture of the Pacific white shrimp, Litopenaeus vannamei
Provedor de dados: 96
Autores: Leite,Jordana Sampaio; Melo,Caio Servulo Batista; Nunes,Alberto Jorge Pinto.
ABSTRACT This study was conducted to evaluate the effect of rice byproducts on water quality, microbial community, and growth performance of L. vannamei juveniles. Shrimp of 0.98±0.10 g body weight (BW) were reared in 49 tanks of 1.5 m3 under 127 animals m−2 for 77 days. Rice bran, rice grits, and rice hulls were mixed into five different fertilizers varying their fiber content (90, 110, 150, 200, and 250 g kg−1) and compared against sugarcane molasses (MO) and unfertilized tanks (UNF). Rice byproducts and MO were applied in water three times a week at a fixed rate of 4.5 g m−3. Water salinity, pH, temperature, and dissolved oxygen reached 43±2 g L−1, 8.03±0.32, 30.2±0.90 °C, and 5.03±0.53 mg L−1, respectively. Settleable solids (SS) were higher in tanks...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial community; Organic fertilization; Shrimp growth performance.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982020000100202

Soil health: looking for suitable indicators. What should be considered to assess the effects of use and management on soil health?
Provedor de dados: 63
Autores: Cardoso,Elke Jurandy Bran Nogueira; Vasconcellos,Rafael Leandro Figueiredo; Bini,Daniel; Miyauchi,Marina Yumi Horta; Santos,Cristiane Alcantara dos; Alves,Paulo Roger Lopes; Paula,Alessandra Monteiro de; Nakatani,André Shigueyoshi; Pereira,Jamil de Moraes; Nogueira,Marco Antonio.
Soil Health refers to the ecological equilibrium and the functionality of a soil and its capacity to maintain a well balanced ecosystem with high biodiversity above and below surface, and productivity. To understand and use soil health as a tool for sustainability, physical, chemical, and biological properties must be employed to verify which respond to the soil use and management within a desired timescale. Attributes with a rapid response to natural or anthropogenic actions are considered good indicators of soil health. Among the physical indicators, soil texture, aggregation, moisture, porosity, and bulk density have been used, while among chemical indicators total C and N, mineral nutrients, organic matter, cation exchange capacity, among others are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Land-use; Microbial community; Soil fauna; Soil quality; Sustainability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000400009

Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs - DOI: 10.4025/actascibiolsci.v32i1.7575
Provedor de dados: 3
Autores: Silva, Ísis Serrano; UNICAMP; Menezes, Cristiano Ragagnin de; UNICAMP; Faria, Andréia Fonseca de; UNICAMP; Costa, Josemar Gurgel da; UFRN; Pepper, Susan; The University of Melbourne; Britz, Margaret; The University of Melbourne; Durrant, Lucia Regina; UNICAMP.
Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7575

Quantitative analysis of Terminal Restriction Fragment Length Polymorphism (T-RFLP) microbial community profiles: peak height data showed to be more reproducible than peak area
Provedor de dados: 58
Autores: Caffaro-Filho,Roberto A.; Fantinatti-Garboggini,Fabiana; Durrant,Lucia R.
Terminal Restriction Fragment Length Polymorphism (T-RFLP) is a culture-independent fingerprinting method for microbial community analysis. Profiles generated by an automated electrophoresis system can be analysed quantitatively using either peak height or peak area data. Statistical testing demontrated that peak height data showed to be more reproducible than peak area data.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Terminal Restriction Fragment Length Polymorphism (T-RFLP); Molecular analysis; Microbial community.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400027

Bacterial community analysis of the water surface layer from a rice-planted and an unplanted flooded field
Provedor de dados: 58
Autores: Scavino,Ana Fernández; Menes,Javier; Ferrando,Lucía; Tarlera,Silvana.
The bacterial communities in floodwater, from a rice-planted and an unplanted field were characterized at the beginning (flooding stage) and at the end (harvest stage) of the rice cropping cycle. Most probable number estimations and plate counts of aerobic and anaerobic heterotrophic bacteria and of several metabolic bacterial groups (methanogens, sulfate-reducers, anaerobic sulfur and nonsulfur phototrophs, denitrifiers and ammonifiers) were similar in rice and unplanted floodwater at both sampling times. The analysis of denitrifiers and methanogens by fluorescent in situ hybridization revealed a shift in the phylogenetic affiliation only of the former group in the rice-planted floodwater. Terminal restriction fragment length polymorphism of 16S rRNA gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Floodwater; Rice; Microbial community; T-RFLP.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200023

Volatile fatty acids influence on the structure of microbial communities producing PHAs
Provedor de dados: 58
Autores: Ciesielski,Slawomir; Przybylek,Grzegorz.
Polyhydroxyalkanoates (PHAs) can be produced by microorganisms and are a biodegradable alternative to fossil-fuel based plastics. Currently, the focus is on reducing production costs by exploring alternative substrates for PHAs production, and on producing copolymers which are less brittle than monomers. Accordingly, this study used a substrate consisting of wastewater from waste-glycerol fermentation, supplemented with different amounts of acetic and propionic acids. These substrates were used to feed mixed microbial communities enriched from activated sludge in a sequencing batch reactor. A reactor supplemented with 2 mL of acetic acid produced 227.8 mg/L of a homopolymer of hydroxybutyrate (3HB); 4 mL of acetic acid produced 279.8 mg/L 3HB; whereas 4 mL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biopolymers; Microbial community; Polyhydroxyalkanoates; RISA; Thauera sp.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200005

Comparative metagenome of a stream impacted by the urbanization phenomenon
Provedor de dados: 58
Autores: Medeiros,Julliane Dutra; Cantão,Maurício Egídio; Cesar,Dionéia Evangelista; Nicolás,Marisa Fabiana; Diniz,Cláudio Galuppo; Silva,Vânia Lúcia; Vasconcelos,Ana Tereza Ribeiro de; Coelho,Cíntia Marques.
Abstract Rivers and streams are important reservoirs of freshwater for human consumption. These ecosystems are threatened by increasing urbanization, because raw sewage discharged into them alters their nutrient content and may affect the composition of their microbial community. In the present study, we investigate the taxonomic and functional profile of the microbial community in an urban lotic environment. Samples of running water were collected at two points in the São Pedro stream: an upstream preserved and non-urbanized area, and a polluted urbanized area with discharged sewage. The metagenomic DNA was sequenced by pyrosequencing. Differences were observed in the community composition at the two sites. The non-urbanized area was overrepresented by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic environment; Urbanization; Microbial community; Metagenomic.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400835