Consulta: subjectFacets:"Microdialysis"
Registros recuperados: 5
Data/hora: 02/06/2024 12:42:42

Neurotransmitter evaluation in the hippocampus of rats after intracerebral injection of TsTX scorpion toxin
Provedor de dados: 80
Autores: Nencioni,ALA; Barreto,SA; Lebrun,I; Florio,JC; Lourenço,GA; Dorce,VAC.
TsTX is an α-type sodium channel toxin that stimulates the discharge of neurotransmitters from neurons. In the present study we investigated which neurotransmitters are released in the hippocampus after TsTX injection and if they are responsible for electrographic or histopathological effects. Microdialysis revealed that the toxin increased glutamate extracellular levels in the hippocampus; however, levels of gamma-aminobutyric acid (GABA), glycine, 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were not significantly altered. Neurodegeneration in pyramidal cells of hippocampus and electroencephalographic alterations caused by the toxin were blocked by pretreatment with riluzole, a glutamate release...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Scorpion toxin; Microdialysis; Hippocampus; Neurotransmitters; Neurodegeneration; Riluzole.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200007

Immunoreactivity for Gonadotropin-Releasing Hormone in Microdialyzed Perfusates of Bovine Mature Follicles In Vitro
Provedor de dados: 19
Autores: Ozawa, Takahiro; Acosta, Tomas J.; Nakamura, Tadashi; Miyamoto, Akio; 中村, 正; 宮本, 明夫.
The presence of gonadotropin-releasing hormone (GnRH)-like protein has been previously demonstrated in the bovine follicle. This GnRH-like protein was purified and concluded to be histone H2A. However, neither GnRH peptide nor specific GnRH-immunoreactivity (GnRH-IR) has been demonstrated in the bovine follicle so far. Thus, this study focused on the detection of specific GnRH-IR using the second-antibody enzyme immunoassay in isolated bovine mature follicles, and on an examination of the direct effect of luteinizing hormone (LH), endothelin-1 (ET-1), and cytokines on the GnRH-IR using an in vitro microdialysis system (MDS). We further examined a cross-reactivity of the GnRH antibody with bovine histone H2A. GnRH-IR was detected in microdialyzed perfusate...
Palavras-chave: GnRH; Immunoreactivity; Microdialysis; Follicle; Cow.
Ano: 2000
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2970

Nitric oxide modulation of the hypothalamo-neurohypophyseal system
Provedor de dados: 56
Autores: Kadekaro,M.
Nitric oxide (NO), a free radical gas produced endogenously from the amino acid L-arginine by NO synthase (NOS), has important functions in modulating vasopressin and oxytocin secretion from the hypothalamo-neurohypophyseal system. NO production is stimulated during increased functional activity of magnocellular neurons, in parallel with plastic changes of the supraoptic nucleus (SON) and paraventricular nucleus. Electrophysiological data recorded from the SON of hypothalamic slices indicate that NO inhibits firing of phasic and non-phasic neurons, while L-NAME, an NOS inhibitor, increases their activity. Results from measurement of neurohypophyseal hormones are more variable. Overall, however, it appears that NO, tonically produced in the forebrain,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-NAME; Vasopressin; Oxytocin; CGMP; ODQ; Microdialysis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400001

Comparative effects of organic and inorganic mercury on in vivo dopamine release in freely moving rats
Provedor de dados: 56
Autores: Faro,L.R.F.; Rodrigues,K.J.A.; Santana,M.B.; Vidal,L.; Alfonso,M.; Durán,R.
The present study was carried out in order to compare the effects of administration of organic (methylmercury, MeHg) and inorganic (mercury chloride, HgCl 2 ) forms of mercury on in vivo dopamine (DA) release from rat striatum. Experiments were performed in conscious and freely moving female adult Sprague-Dawley (230-280 g) rats using brain microdialysis coupled to HPLC with electrochemical detection. Perfusion of different concentrations of MeHg or HgCl 2 (2 µL/min for 1 h, N = 5-7/group) into the striatum produced significant increases in the levels of DA. Infusion of 40 µM, 400 µM, or 4 mM MeHg increased DA levels to 907 ± 31, 2324 ± 156, and 9032 ± 70% of basal levels, respectively. The same concentrations of HgCl 2 increased DA levels to 1240 ± 66,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Methylmercury; Mercury chloride; Dopamine release in vivo; Microdialysis; Mercury determination; Striatum.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000009

Basic considerations in the dermatokinetics of topical formulations
Provedor de dados: 60
Autores: Nair,Anroop; Jacob,Shery; Al-Dhubiab,Bandar; Attimarad,Mahesh; Harsha,Sree.
Assessing the bioavailability of drug molecules at the site of action provides better insight into the efficiency of a dosage form. However, determining drug concentration in the skin layers following topical application of dermatological formulations is a great challenge. The protocols followed in oral formulations could not be applied for topical dosage forms. The regulatory agencies are considering several possible approaches such as tape stripping, microdialysis etc. On the other hand, the skin bioavailability assessment of xenobiotics is equally important for topical formulations in order to evaluate the toxicity. It is always possible that drug molecules applied on the skin surface may transport thorough the skin and reaches systemic circulation....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dermatological formulations/pharmacokinetics; Dermatological formulations/toxicity; Dermatokinetics; Skin/metabolism; Microdialysis; Tape stripping.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000300004