Consulta: subjectFacets:"Micronucleus test"
Registros recuperados: 25
Data/hora: 08/06/2024 15:43:34

Evaluation of genotoxicity through micronuclei test in workers of car and battery repair garages
Provedor de dados: 74
Autores: Martino-Roth,M.G.; Viégas,J.; Amaral,M.; Oliveira,L.; Ferreira,F.L.S.; Erdtmann,B.
In this study, the micronuclei test (MNT) was applied in exfoliated cells of buccal mucosa, in order to evaluate the genotoxic risk associated with occupational exposure of mechanics, storage battery renovation workers, and car painters. For each individual, 3000 exfoliated buccal cells were analyzed. There was a significantly higher frequency of micronucleated cells (MNC) in the exposed workers than in controls. Smoking and drinking habits, age, and working time did not represent significant factors in terms of increasing the production of micronuclei (MN), when the control and the exposed groups were compared. These results allowed to conclude that the studied individuals belong to a risk group and should periodically undergo biological monitoring and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Occupational exposure.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400021

Assessment of the genotoxic impact of pesticides on farming communities in the countryside of Santa Catarina State, Brazil
Provedor de dados: 74
Autores: Salvagni,Jaqueli; Ternus,Raquel Zeni; Fuentefria,Alexandre Meneghello.
The aim of this study was to assess the use of pesticides on farms located in the Lambedor River watershed in Guatambu, State of Santa Catarina, as well as to determine, by micronucleus testing, the risk of genotoxic impact. Samples from locally collected Cyprinus carpio, Hypostomus punctatus, Rhamdia quelen and Oreochromis niloticus gave evidence of a mean increase in micronuclei frequency from 6.21 to 13.78 in 1,000 erythrocytes, a clear indication of the genotoxic potenciality of pesticide residues in regional dams, and their significant contribution to local environmental contamination.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides; Environment; Micronucleus test.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100021

Clastogenicity of Piper cubeba (Piperaceae) seed extract in an in vivo mammalian cell system
Provedor de dados: 74
Autores: Junqueira,Adriana Pereira Freire; Perazzo,Fabio Ferreira; Souza,Gustavo Henrique Bianco; Maistro,Edson Luis.
The plant Piper cubeba is widely distributed in tropical and subtropical regions and is used medically for various purposes but has not yet been evaluated for genotoxicity. We used male and female Swiss mice and Wistar rats and the comet assay and micronucleus test to investigate the mutagenic potential of a crude extract of P. cubeba seeds. The rodents were administered 0.5 g kg-1, 1.0 g kg-1 and 1.5 g kg-1 of the extract by gavage. For the Swiss mice, peripheral blood was collected 24 h after treatment for the comet assay, and at 48 and 72 h for the micronucleus test. For the Wistar rats, peripheral blood and hepatic cells were collected for the comet assay and bone marrow cells were collected for the micronucleus test 24 h after treatment. At 1.5 g...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Piper cubeba; Single cell gel electrophoresis (SCGE).
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400025

Evaluation of the genotoxic and antigenotoxic potential of Melissa officinalis in mice
Provedor de dados: 74
Autores: Carvalho,Natália Cassettari de; Corrêa-Angeloni,Maria Júlia Frydberg; Leffa,Daniela Dimer; Moreira,Jeverson; Nicolau,Vanessa; Amaral,Patrícia de Aguiar; Rossatto,Ângela Erna; Andrade,Vanessa Moraes de.
Melissa officinalis (L.) (Lamiaceae), a plant known as the lemon balm, is native to the east Mediterranean region and west Asia. Also found in tropical countries, such as Brazil, where it is popularly known as "erva-cidreira" or "melissa", it is widely used in aqueous- or alcoholic-extract form in the treatment of various disorders. The aim was to investigate in vivo its antigenotoxicity and antimutagenicity, as well as its genotoxic/mutagenic potential through comet and micronucleus assaying. CF-1 male mice were treated with ethanolic (Mo-EE) (250 or 500 mg/kg) or aqueous (Mo-AE) (100 mg/kg) solutions of an M. officinalis extract for 2 weeks, prior to treatment with saline or Methyl methanesulfonate (MMS) doses by intraperitoneal injection. Irrespective...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melissa officinalis; Comet assay; Micronucleus test; Genotoxicity; Antigenotoxicity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200021

Genotoxicity of Nicotiana tabacum leaves on Helix aspersa
Provedor de dados: 74
Autores: Silva,Fernanda R. da; Erdtmann,Bernardo; Dalpiaz,Tiago; Nunes,Emilene; Ferraz,Alexandre; Martins,Tales L.C.; Dias,Johny F.; Rosa,Darlan P. da; Porawskie,Marilene; Bona,Silvia; Silva,Juliana da.
Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Genotoxicity; Micronucleus test; Helix aspersa; Nicotiana tabacum; Tobacco leaves.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200018

Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining
Provedor de dados: 74
Autores: Kvitko,Katia; Bandinelli,Eliane; Henriques,João A.P.; Heuser,Vanina D.; Rohr,Paula; Silva,Fernanda R. da; Schneider,Naye Balzan; Fernandes,Simone; Ancines,Camile; Silva,Juliana da.
Our mutagenesis group has been studying with important economic drivers of our state, such as agriculture, the footwear and leather industry and open-cast coal mining. Working conditions in these sectors have potentially harmful to humans. The aim of these studies is to determine the health risk of workers by biomonitoring subjects exposed to genotoxic agents. The main results of our studies with vineyard farmers we observed a high rate of MN and DNA damage in individuals exposed to pesticides (p &lt; 0.001). In addition, some effects of genetic polymorphisms in the modulation of MN results were observed in this group. Tobacco farmers were also evaluated at different crop times. The results showed a significant increase in the Damage index and frequency in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Occupational exposure; Comet assay; Micronucleus test; Pesticide; Coal dust.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600022

No clastogenic activity of Caesalpinia ferrea Mart. (Leguminosae) extract on bone marrow cells of Wistar rats
Provedor de dados: 74
Autores: Souza,Andréa Bêcco de; Souza,Lilian Mara Silva; Carvalho,José Carlos Tavares; Maistro,Edson Luis.
Extracts of the fruits of Caesalpinia ferrea Mart. (Leguminosae) are widely consumed in folkloric medicine in Brazil and several other countries without any genetic toxicity evaluation. In this study we investigated, the clastogenic and cytotoxic potential of the crude aqueous extract of the fruits of C. ferrea in Wistar rat bone marrow cells using the micronucleus and chromosomal aberration test systems. The animals were treated by gavage with 3 concentrations of the extract, 500, 1000 and 1500 mg/kg, and cyclophosphamide 30 mg/kg. Bone marrow cells were collected 24 h after the treatment. There was no statistically significant difference in the mean of micronucleated polychromatic erythrocytes (MNPCE), mean number of chromosomal aberrations or mitotic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Caesalpinia ferrea; Micronucleus test; Chromosome aberrations.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200028

Micronuclei induction in Rana catesbeiana tadpoles by the pyrethroid insecticide lambda-cyhalothrin
Provedor de dados: 74
Autores: Campana,Marcela Alejandra; Panzeri,Ana María; Moreno,Víctor Jorge; Dulout,Fernando Noel.
Pyrethroid lambda-cyhalothrin genotoxicity was evaluated using the micronucleus test in Rana catesbeiana tadpoles. The effects of concentration and exposure time on the micronuclei frequency were studied in blood smears obtained from tadpoles exposed to four concentrations (0.02, 0.1, 0.2 and 0.4 mg/L) of the compound for 24, 48, 72 and 96 h and 8, 15, 20 and 30 days. As a positive control, tadpoles were exposed to cyclophosphamide (5 mg/L). The micronucleated cell frequency was expressed per 1,000 cells. R. catesbeiana tadpoles exposed to increasing concentrations of lambda-cyhalothrin showed an increase in the micronuclei frequency in peripheral blood. Tadpoles exposed to cyclophosphamide (CP) also showed a significant increase in micronucleated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genotoxicity; Micronucleus test; Lambda-cyhalothrin; Tadpoles.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100016

Assessment of genetic integrity, splenic phagocytosis and cell death potential of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid and its effect when combined with commercial chemotherapeutics
Provedor de dados: 74
Autores: Oliveira,Rodrigo Juliano; da Cruz Leite Santos,Naiara; Pesarini,João Renato; de Oliveira,Beatriz Carneiro; Berno,Claudia Rodrigues; de Araújo,Flávio Henrique Souza; da Silveira,Ingridhy Ostaciana Maia Freitas; Nascimento,Raquel Oliveira; Brochado Antoniolli-Silva,Andréia Conceição Milan; Duenhas Monreal,Antônio Carlos; Beatriz,Adilson; de Lima,Dênis Pires; da Silva Gomes,Roberto.
Abstract The increased incidence of cancer and its high treatment costs have encouraged the search for new compounds to be used in adjuvant therapies for this disease. This study discloses the synthesis of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid (IR-01) and evaluates not only the action of this compound on genetic integrity, increase in splenic phagocytosis and induction of cell death but also its effects in combination with the commercial chemotherapeutic agents doxorubicin, cisplatin and cyclophosphamide. IR-01 was designed and synthesized based on two multifunctionalyzed structural fragments: 4-aminoantipyrine, an active dipyrone metabolite, described as an antioxidant and anti-inflammatory agent; and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Splenic phagocytosis; Comet assay; Micronucleus test; Cell death; Chemoprevention.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100154

Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects
Provedor de dados: 74
Autores: Mendonça,Leonardo Meneghin; Machado,Carla da Silva; Teixeira,Cristiane Cardoso Correia; Freitas,Luis Alexandre Pedro de; Bianchi,Maria Lourdes Pires; Antunes,Lusânia Maria Greggi.
Abstract Curcumin (CMN) is the principal active component derived from the rhizome of Curcuma longa (Curcuma longa L.). It is a liposoluble polyphenolic compound that possesses great therapeutic potential. Its clinical application is, however, limited by the low concentrations detected following oral administration. One key strategy for improving the solubility and bioavailability of poorly water-soluble drugs is solid dispersion, though it is not known whether this technique might influence the pharmacological effects of CMN. Thus, in this study, we aimed to evaluate the antioxidant and antigenotoxic effects of CMN formulated in a solid dispersion (CMN SD) compared to unmodified CMN delivered to Wistar rats. Cisplatin (cDDP) was used as the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Curcuma longa; Antigenotoxicity; Micronucleus test; DNA damage; Comet assay.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400490

Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[a]pyrene and cyclophosphamide
Provedor de dados: 74
Autores: Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E.
Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643

Increased micronucleated cell frequency related to exposure to radiation emitted by computer cathode ray tube video display monitors
Provedor de dados: 74
Autores: Carbonari,Karina; Gonçalves,Luciane; Roth,Daniela; Moreira,Patrick; Fernández,Ricardo; Martino-Roth,Maria da Graça.
It is well recognized that electromagnetic fields can affect the biological functions of living organisms at both cellular and molecular level. The potential damaging effects of electromagnetic fields and very low frequency and extremely low frequency radiation emitted by computer cathode ray tube video display monitors (VDMs) has become a concern within the scientific community. We studied the effects of occupational exposure to VDMs in 10 males and 10 females occupationally exposed to VDMs and 20 unexposed control subjects matched for age and sex. Genetic damage was assessed by examining the frequency of micronuclei in exfoliated buccal cells and the frequency of other nuclear abnormalities such as binucleated and broken egg cells. Although there were no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Micronucleus test; Mutagenicity; Electromagnetic fields.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300024

Evaluation of the genotoxic potential of the Hypericum brasiliense (Guttiferae) extract in mammalian cell system in vivo
Provedor de dados: 74
Autores: Espósito,Anderson Victorino; Pereira,Danielli Maria Vieira; Rocha,Leandro Machado; Carvalho,José Carlos Tavares; Maistro,Edson Luis.
Plants of the genus Hypericum, long used in folk medicine, contain active compounds which present, anti-septic, diuretic, digestive, expectorant, vermifugal, anti-depressive and other properties. The possible clastogenic effect of a H. brasiliense extract was tested in vivo on the bone marrow cells of Wistar rats. The extract was administered by gavage at doses of 50, 150 and 300 mg/kg body weight. Experimental and control animals were submitted to euthanasia 24 h after the treatment for micronucleus (MN) and chromosome preparations. H. brasiliense extract did not induce statistically significant increases in the average numbers of MN or chromosome aberrations in the test systems employed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypericum brasiliense; Micronucleus test; Chromosome aberrations.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100026

In vivo evaluation of the mutagenic potential and phytochemical characterization of oleoresin from Copaifera duckei Dwyer
Provedor de dados: 74
Autores: Maistro,Edson Luis; Carvalho,José Carlos Tavares; Cascon,Vera; Kaplan,Maria Auxiliadora Coelho.
We characterized the chemical constituents of Copaifera duckei oleoresin and used dermal application to Wistar rats to evaluated its possible mutagenic and cytotoxic activities on peripheral blood reticulocytes and bone marrow cells. Chemical characterization of the oleoresin revealed the presence of sesquiterpene hydrocarbons, an unidentified neutral diterpene and diterpene acids. To evaluate mutagenicity evaluation the rats were treated with 10, 25 and 50% of the LD50 dose of the oleoresin for three consecutive days and peripheral blood collected after 0, 24, 48 and 72 h for micronucleus analysis. The rats were humanly sacrificed 24 hours after the last treatment and chromosome preparations made using standard techniques. At the three concentrations and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copaifera duckei (Caesalpinaceae); Phytochemical characterization; Micronucleus test; Chromosome aberrations; Cytotoxic effect.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500028

Effect of watercress on induced DNA damage, DNA repair and p-glycoprotein activity in human lymphocytes
Provedor de dados: 46
Autores: Casanova,N.A; Wagner,M.L; López Nigro,M.M; Carballo,M.A.
Cruciferae vegetables are widely studied due to their potential anticarcinogenic properties. In the present work we analyzed the antigenotoxic effect of watercress (Nasturtium officinale, W. Aiton), a member of this family. The vegetal was processed, and the juice obtained was evaluated in relation with genetic material protection and DNA repair capacity against two deleterious agents: mitomycin C and hydrogen peroxide. The antigenotoxic properties were analyzed through cytokinesis block micronucleus test and comet assay (standard and with endonuclease), respectively. Besides, we tested the functionality of P-glycoprotein in human lymphocytes exposed to the whole extract. Watercress had no effect on mitomycin cytostatic effect (p&amp;gt;0.05) and although...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemopreventive effects; Comet assay; Micronucleus test; Transporters; Nasturtium officinale.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332014000300005

Mutagenicity induced by the hydroalcoholic extract of the medicinal plant Plathymenia reticulata Benth
Provedor de dados: 80
Autores: Della Torre,A; Albuquerque,LBL; Farrapo,NM; Oshima-Franco,Y; Santos,MG; Tavares,RVS; Rodas,ACD; Dal Belo,CA; Cardoso,CRP; Varanda,EA; Groppo,FC; Lopes,PS.
Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ames test; Micronucleus test; Mutagenicity; Plant extract; Plathymenia reticulata.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010

Acute copper toxicity in juvenile fat snook Centropomus parallelus (Teleostei: Centropomidae) in sea water
Provedor de dados: 83
Autores: Oliveira,Bruno L.; Fernandes,Luiz F. Loureiro; Bianchini,Adalto; Chippari-Gomes,Adriana R.; Silva,Bruno F.; Brandão,Geisamanda P.; Gomes,Levy C.
Three experiments were designed to assess the accumulation and acute toxicity of copper (Cu) in juvenile fat snook Centropomusparallelus. The first experiment was performed to determine the 96-h lethal concentration (LC50) of Cu. The second experiment was designed to assess the effects of sublethal concentrations of Cu (0.47 and 0.94 mg/L), while the third one allowed us to test the recovery capacity of fish exposed to the sublethal concentrations Cu and kept in sea water without Cu addition. The LC50value for Cu was found to be 1.88 mg/L Cu. Fish exposed to the sublethal concentrations of Cu showed a significant accumulation of Cu in gills at 96 h respect to the control ones (0.43 µg/g Cu). No significant difference was observed in the accumulation of Cu...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aquatic toxicology; Comet assay; Gills; Lethal toxicity; Micronucleus test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400845

Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides
Provedor de dados: 42
Autores: ALVES,JODEL S.; SILVA,FERNANDA R. DA; SILVA,GABRIELI F. DA; SALVADOR,MIRIAN; KVITKO,KÁTIA; ROHR,PAULA; SANTOS,CARLA E.I. DOS; DIAS,JOHNNY F.; HENRIQUES,JOÃO A.P.; SILVA,JULIANA DA.
ABSTRACT Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profile was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pesticides; Comet assay; Micronucleus test; Superoxide dismutase activity; PON1 gene.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100349

Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
Provedor de dados: 42
Autores: SPERA,KAMILLE D.; FIGUEIREDO,PATRÍCIA A.; SANTOS,PAMELA C.E; BARBOSA,FERNANDO C.; ALVES,CAIO P.; DOKKEDAL,ANNE LÍGIA; SALDANHA,LUIZ LEONARDO; SILVA,LUCIANA P.; FIGUEIREDO,CARLOS ROGÉRIO; FERREIRA,PAULO CESAR; SILVA,REGILDO M.G. DA.
Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: B16F10 melanoma cells; Micronucleus test; Chromosomal aberration; DPPH radical scavenging; HPLC-DAD.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617

Assessment of Duguetia furfuracea genotoxic and cytotoxic activity in bacteria and mice
Provedor de dados: 42
Autores: Silva,Carolina R.; Vieira,Pabline M.; Santos,Suzana C.; Chen-Chen,Lee.
Duguetia furfuracea (St. Hil.) Benth &amp;amp; Hook f. (1862), popularly known as "sofre-do-rim-quem-quer" and "araticum-seco", is a shrub of the Annonaceae family that occurs in several regions of Brazil. In folk medicine the infusion of its leaves and twigs is used to treat rheumatism and renal colic, whereas the seed powder is mixed with water to treat pediculosis. Previous studies have described biological activities of this plant with cytotoxic, antitumoral, trypanocidal, leishmanicidal, antiplasmodial and antiprotozoal effects. In the present work, genotoxicity and cytotoxicity of Duguetia furfuracea lyophilized leaf extract were evaluated using the prophage λ induction test (SOS-Inductest) and mouse bone marrow micronucleus test. Our results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytotoxicity; Duguetia furfuracea; Genotoxicity; Micronucleus test; SOS-Inductest.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000100015

DNA damage in children and adolescents with cardiovascular disease risk factors
Provedor de dados: 42
Autores: Kliemann,Mariele; Prá,Daniel; Müller,Luiza L.; Hermes,Liziane; Horta,Jorge A.; Reckziegel,Miriam B.; Burgos,Miria S.; Maluf,Sharbel W.; Franke,Silvia I.R.; Silva,Juliana da.
The risk of developing cardiovascular disease (CVD) is related to lifestyle (e.g. diet, physical activity and smoking) as well as to genetic factors. This study aimed at evaluating the association between CVD risk factors and DNA damage levels in children and adolescents. Anthropometry, diet and serum CVD risk factors were evaluated by standard procedures. DNA damage levels were accessed by the comet assay (Single cell gel electrophoresis; SCGE) and cytokinesis-blocked micronucleus (CBMN) assays in leukocytes. A total of 34 children and adolescents selected from a population sample were divided into three groups according to their level of CVD risk. Moderate and high CVD risk subjects showed significantly higher body fat and serum CVD risk markers than low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular diseases; Micronucleus test; Comet assay; Diet; Anthropometry; Child; Adolescent.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000300025

Hibiscus acetosella extract protects against alkylating agent-induced DNA damage in mice
Provedor de dados: 42
Autores: VILELA,THAIS C.; LEFFA,DANIELA D.; DAMIANI,ADRIANI P.; DAMAZIO,DAIANE DAL COL; MANENTI,ALINE V.; CARVALHO,TIAGO JOSÉ G.; RAMLOV,FERNANDA; AMARAL,PATRICIA A.; ANDRADE,VANESSA M. DE.
Abstract Hibiscus acetosella was shown to exert beneficial effects in humans and animal models however, the effects of this plant on DNA are unknown. The aim of this study was to determine the antigenotoxic and antimutagenic effects of H. acetosella extracts on alkylating agent methyl methanesulfonate (MMS) in vivo in mice. Initially, we performed analysis of phenolic compounds in extracts of H. acetosella by high-performance liquid chromatography (HPLC). Next, mice were divided into 8 groups and treated with distilled water or plant extract (0.1 ml/10 g) by gavage for 15 days, followed by intraperitoneal (ip) administration of saline solution or MMS (40 mg/Kg b.w) on day 16. Caffeic acid, following by gallic acid, gallocatechin, coumaric acid, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hibiscus acetosella; Antigenotoxicity; Comet assay; Antimutagenicity; Micronucleus test.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000603165

Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
Provedor de dados: 48
Autores: Ellahueñe,Manuel F; Pérez-Alzola,L Patricia; Olmedo,M Isabel.
Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but...
Tipo: Journal article
Palavras-chave: Dominant lethal mutation; Ethanol; Genotoxicity; Micronucleus test.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100004

Evaluation of inorganic and organic bone components after application of an apatite-coated Al2O3 implants as scaffolds for bone repair
Provedor de dados: 52
Autores: Maia Filho,Antonio Luiz Martins; Amaral,Fabrício Pires de Moura do; Martin,Airton Abrahão; Soares,Luís Eduardo Silva.
The aim of this work was to study the influence of uncoated and apatite-coated Al2O3 implants on bone regeneration after 30 days of surgery in New Zealand white rabbits. Twelve samples of Al2O3 implants were prepared and half of them (n = 6) were apatite-coated by the modified biomimetic method. Three experimental groups were tested as Group C - control, surgery procedure without cerami c implant, Group Ce - uncoated Al 2O3 implants (n = 6) and Group CeHA - apatite-coated Al2O3 implants (n = 6). The mineralization of the Al2O3 implants was analyzed in bone fragments using FT-Raman spectroscopy. Raman peaks at 959 cm-1 and 2940 cm-1 evaluated the inorganic and organic bone content, respectively. In vivo citotoxicity was analyzed using micronucleus test....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone regeneration; Alumina foams; Apatite coating; FT-Raman spectroscopy; Micronucleus test.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300004

The mutagenic and antimutagenic effects of the traditional phytoestrogen-rich herbs, Pueraria mirifica and Pueraria lobata
Provedor de dados: 56
Autores: Cherdshewasart,W.; Sutjit,W.; Pulcharoen,K.; Chulasiri,M.
Pueraria mirifica is a Thai phytoestrogen-rich herb traditionally used for the treatment of menopausal symptoms. Pueraria lobata is also a phytoestrogen-rich herb traditionally used in Japan, Korea and China for the treatment of hypertension and alcoholism. We evaluated the mutagenic and antimutagenic activity of the two plant extracts using the Ames test preincubation method plus or minus the rat liver mixture S9 for metabolic activation using Salmonella typhimurium strains TA98 and TA100 as indicator strains. The cytotoxicity of the two extracts to the two S. typhimurium indicators was evaluated before the mutagenic and antimutagenic tests. Both extracts at a final concentration of 2.5, 5, 10, or 20 mg/plate exhibited only mild cytotoxic effects. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phytoestrogen; Pueraria mirifica; Pueraria lobata; Mutagenic test; Antimutagenic test; Micronucleus test.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900008