Consulta: subjectFacets:"Microscopy"
Registros recuperados: 34
Data/hora: 05/06/2024 05:47:03

Surimi de chinchard ( Trachurus trachurus L. ) - Influence de conditions technologiques sur des caractéristiques de texture du produit après traitement thermique
Provedor de dados: 5
Autores: Verrez-bagnis, Veronique.
Surimi, washed fish mince after grinding with salt, has a firm and elastic gel texture or “ashi” after thermal processing. The major objectives of this study on horse mackerel (Trachurus trachurus) surimi are to optimize the technological conditions in the aim to obtain the best ashi, to determine the influence of frozen storage and of egg white and potata starch addition, and to establish the preventive effect of incorporation into surimi of different combinations of cryoprotectants and NaC1. Gel texture is, by order importance, dependent on cooking temperature, length of setting and cooking and length of grinding of surimi with texture additives after thawing. Changes of gelling properties after 6 months at - 30°C of frozen st orage are limited. The...
Tipo: Text
Palavras-chave: Fish; Horse mackerel (Trachurus trachurus L.); Surimi; Kamaboko; Myofibrillar proteins; Gelation; Thermal coagulation; Texture; Optimisation method; Technological treatments; Cryoprotectors; Texture ingredients; Microscopy; Water retention capacity.
Ano: 1987
URL: http://archimer.ifremer.fr/doc/00134/24507/22535.pdf

Interet de la microscopie analytique pour l''etude d''impact de metaux traces et de terres rares sur le milieu vivant. Application a l''etude d''une zone polluee: La Baie de Seine
Provedor de dados: 5
Autores: Chassard Bouchaud, C; Noel, Philippe; Hubert, M; Hallegot, P.
To study bioaccumulation and metabolism of trace metals (silver-lead-copper-iron-uranium) and rare earths (lanthanium) in estuarine organisms (Crangon crangon, Mytilus edulis, Abra Alba, Cutellus pellucidus, Owenia furiformis ) the authors use in situ microanalysis associated with electronic or photonic microscopy.
Tipo: Text
Palavras-chave: Rare earths; Trace metals; Bioaccumulation; Pollution effects; Microscopy.
Ano: 1985
URL: http://archimer.ifremer.fr/doc/1985/acte-1305.pdf

Redescription and phylogenetic analyses of Durchoniella spp. (Ciliophora, Astomatida) associated with the polychaete Cirriformia tentaculata (Montagu, 1808)
Provedor de dados: 5
Autores: Sauvadet, Anne-laure; Lynn, Denis H.; Roussel, Erwan; Le Panse, Sophie; Bigeard, Estelle; Schrevel, Joseph; Guillou, Laure.
Microscopic and phylogenetic analyses were performed on endocommensal astome ciliates retrieved from the middle intestine of a marine cirratulid polychaete, Cirriformia tentaculata, collected in the bay of Roscoff (English Channel, Northwest French coast) and on the Southwest English coast. Three morphotypes of the astome genus Durchoniella were identified, two corresponding to described species (the type species Durchoniella brasili (Léger and Duboscq, 1904) de Puytorac, 1954 and Durchoniella legeriduboscqui de Puytorac, 1954) while a third morphotype remains undescribed. Their small subunit (SSU) rRNA gene sequences showed at least 97.2% identity and phylogenetic analyses grouped them at the base of the subclass Scuticociliatia (Oligohymenophorea), as a...
Tipo: Text
Palavras-chave: Astomatida; Ciliophora; Cirratulidae; Endocytoplasmic bacteria; Microscopy; Molecular phylogeny.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00392/50312/50990.pdf

Induction of apoptosis by UV in the flat oyster, Ostrea edulis
Provedor de dados: 5
Autores: Gervais, Ophelie; Renault, Tristan; Arzul, Isabelle.
Apoptosis is a fundamental feature in the development of many organisms and tissue systems. It is also a mechanism of host defense against environmental stress factors or pathogens by contributing to the elimination of infected cells. Hemocytes play a key role in defense mechanisms in invertebrates and previous studies have shown that physical or chemical stress can increase apoptosis in hemocytes in mollusks. However this phenomenon has rarely been investigated in bivalves especially in the flat oyster Ostrea edulis. The apoptotic response of hemocytes from flat oysters, O. edulis, was investigated after exposure to UV and dexamethasone, two agents known to induce apoptosis in vertebrates. Flow cytometry and microscopy were combined to demonstrate that...
Tipo: Text
Palavras-chave: Apoptosis; Hemocyte(s); Oyster; Ostrea edulis; UV; Flow cytometry; Microscopy.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00270/38160/36295.pdf

Ostreopsis lenticularis Y. Fukuyo (Dinophyceae, Gonyaulacales) from French Polynesia (South Pacific Ocean): A revisit of its morphology, molecular phylogeny and toxicity
Provedor de dados: 5
Autores: Chomérat, Nicolas; Bilien, Gwenael; Derrien, Amelie; Henry, Kévin; Ung, André; Viallon, Jérôme; Darius, Hélène Taiana; Mahana Iti Gatti, Clémence; Roué, Mélanie; Hervé, Fabienne; Réveillon, Damien; Amzil, Zouher; Chinain, Mireille.
To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French...
Tipo: Text
Palavras-chave: ITS-5.8S rDNA; LSU rDNA; Microscopy; Ostreopsis lenticularis; Taxonomy; Toxins.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00486/59747/62887.pdf

Cellular Changes in the Placenta in Pregnancies Complicated with Diabetes
Provedor de dados: 77
Autores: Verma,Ranjana; Mishra,Sabita; Kaul,Jagat Mohini.
Placenta is the most accurate record of the infant prenatal experience. After delivery if the placenta is examined minutely, it provides much insight into the prenatal health of the baby and the mother. In diabetic pregnancy, placental weight is higher in comparison to normal pregnancy. To study the cellular differences that might contribute to larger size of placenta, light microscopic analysis was performed on 25 full term placentas, out of which 20 were of Gestational diabetes mellitus (12 controlled on diet, 8 controlled on insulin) and 5 control group. Tissue sections were processed and analyzed. Birth weight of neonate, placental weight, vascular pattern of chorionic blood vessels and site of attachment of umbilical cord were recorded. In the...
Tipo: Journal article
Palavras-chave: Placenta; Gross; Gestational diabetes; Microscopy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100038

Academic Achievement and Perception of Two Teaching Methods in Histology: Light Microscopy and Digital Systems
Provedor de dados: 77
Autores: Becerra G,Daniela; Grob L. P,Melisa; Rodríguez R,Ángel; Barker M,María José; Consiglieri L,Lucas; Ferri G,Giorgio; Sabag S,Natividad.
With the advent of digital systems, the role of the microscope as an irreplaceable instrument in the practical teaching of histology has been called into question. In this study academic performance and student perception for three learning methods was compared: digital systems, microscopy, and microscopy plus digital systems, in the muscle tissue unit of the morphology course for first-year dentistry at the Universidad de los Andes, Santiago, Chile. Ninety-five students were divided into 3 groups: Group 1: individual optical microscopy, Group 2: digital systems (one projector per room), and Group 3: microscopy plus digital systems. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle mounts. Their diagnostic...
Tipo: Journal article
Palavras-chave: Medical education; Histology; Visual learning; Virtual slides; Microscopy.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300001

Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells
Provedor de dados: 77
Autores: Aktas,Ranan Gulhan; Karabay,Gulten; Taskinalp,Oguz; Kutlu,A. Kemal.
Although the structure and the functions of juxtaglomerular cells (JG) have been well defined, there is still a controversy about the secretory mechanisms of renin from these cells. It has been assumed that exocytosis is the main secretory mechanism in these cells in many studies, while others suggest that secretion occurs in a quite different way in these cells. There are several studies suggesting that diacrine secretion, which is very difficult to visualize, might be the other mechanism for secretion of renin. This study is an attempt to find the answers of these questions by identifying the fine structural features of the secretory granules in juxtaglomerular cells. Cyclosporin A (CyA) has been used in the current experimental study since it has...
Tipo: Journal article
Palavras-chave: Juxtaglomerular cells; Secretory granules; Microscopy.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300011

Morphological Study of the Eye Bulb of the Hammerhead Shark, Sphyrna lewini (Elasmobranch: Carcharhinidae)
Provedor de dados: 77
Autores: Muriana,C. B; Vasconcelos,B. V; Leandro,R. M; Malavasi,C. E; Amorim,A. F; Rici,R. E. G; Maria,D. A; Miglino,M. A; Ferreira,A.O.
The hammerhead sharks shows a head laterally expanded with eyes and nostrils on the edges, which gives the species a hammer appearance. Another strand of studies indicates that the hypothesis of head shape alterations for better adaptions in the sensorial system with the development of structures associated to binocular sight and others sensorial organs. Given the variety of characteristics, the study aim was to describe the anatomical constitution of the hammerhead shark eye bulb. The bulb and its annexes exenteration was performed, and further dissection; the morphological description of the muscle insertions and eye bulb components were based on direct observation and were further photographed and catalogued. The eye bulb fragments and its annexes were...
Tipo: Journal article
Palavras-chave: Cartilaginous fish; Chondrichthyes; Vision; Microscopy; Morphology.
Ano: 2017
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100045

Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods
Provedor de dados: 77
Autores: Millas,Ieda; Barros,Mirna Duarte; Curcio,Daniella Franco; Buck,Hudson Sousa; Liquidato,Bianca Maria.
Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density...
Tipo: Journal article
Palavras-chave: Immunohistochemistry; Estrogen receptors; Methods; Microscopy.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007

Tegumentary epithelial glands in the abdomen of virgin and physogastric queens of the stingless bee Scaptotrigona postica Latreille (Meliponini: Trigonina)
Provedor de dados: 82
Autores: Abdalla,Fábio C.; Cruz-Landim,Carminda da; Gracioli,Luciana F.
In the eusocial worker bees, portions of the epithelium of the abdominal epidermis may become hypertrophied and glandular, and produce the wax used to build the brood alveoli. In meliponine bees, the presence of similar epithelium was already observed in the queens, but its function is still unknown. Would this epithelium have homologous function in the workers? May the queens display other functions, besides reproduction, related to those considered exclusive of workers? In order to contribute to solve these questions, a histological and ultrastructural study of the tegumentary epithelial glands of the third tergite was carried out in virgins and physogastric queens of Scaptotrigona postica Latreille. The glandular tegumentary epithelium was easily...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wax; Meliponine; Histology; Microscopy.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000100006

Appearance and Localization of Plasmodium yoelii Circumsporozoite Protein during Sporogony in Anopheles stephensi
Provedor de dados: 19
Autores: Torii, Motomi; Yanagi, Tetsuo; Tsuboi, Takafumi; Kanbara, Hiroji.
Palavras-chave: Circumsporozoite protein; Immunoelectron; Microscopy; Plasmodium yoelii; Sporogony.
Ano: 1992
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/174

Scanning electron microscope studies on some species of the centric diatom genera &lt;i&gt;Thalassiosira&lt;/i&gt; and &lt;i&gt;Coscinodiscus&lt;/i&gt;
Provedor de dados: 27
Autores: Somers, D.
By means of a scanning electron microscope, the submicroscopic structure of four &lt;i&gt;Thalassiosira&lt;/i&gt; species and two &lt;i&gt;Coscinodiscus&lt;/i&gt; species is studied. Five of these taxa are more or less similar in the arrangement of the areoles; the sixth one is aberrant. Besides the description of each species and the description of the submicroscopic structure of the auxospore of &lt;i&gt;Thalassiosira nordenskiöldii&lt;/i&gt;, a classification is given of the six taxa studied.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatoms; Electron microscopy; Microscopy; Coscinodiscus; Thalassiosira.
Ano: 1972
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3457

Effect of an Enzymatic Blend and Yeast on the Performance, Carcass Yield and Histomorphometry of the Small Intestine in Broilers from 21 to 42 Days of Age
Provedor de dados: 89
Autores: Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC.
ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p&lt;0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300

Microstructure and flow properties of lyophilized mango pulp with maltodextrin
Provedor de dados: 91
Autores: Afonso,Marcos R. A.; Rodrigues,Brena K. M.; Costa,José M. C. da; Rybka,Ana C. P.; Wurlitzer,Nedio J.
ABSTRACT The present study had as objective to determine the flow properties and behavior of lyophilized mango pulp powder as a function of different concentrations of maltodextrin. In the samples of the powders contain 5, 10 and 15% of maltodextrin the angle of effective internal friction, wall friction angle, flow index and bulk density were determined. The microstructure was evaluated by scanning electron microscopy. The freeze-dried samples are characterized as having an amorphous structure, and the drying aid used modified the surface of the particles. The flow index was 3.19, 4.28 and 4.53 for samples containing, respectively, 5, 10 and 15% maltodextrin. Increasing the concentration of maltodextrin in the mango pulp decreased the effective angles of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Powdered fruit; Microscopy; Flow index; Powder rheology.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662019000200133

A universal microscope manipulator
Provedor de dados: 92
Autores: Boyadzhiev,Peter S.; Gechev,Tsanko S.; Donev,Atanas D.
A modified and improved model of a mechanical manipulator for observation of pinned and mounted insects is described. This device allows movement of the observed object around three perpendicular axes in the field of vision at all magnifications of stereomicroscopes. The main improvement of this new model is positioning of the guiding knobs for rotating around two of the axes next to each other, allowing faster and easier manipulation of the studied object. Thus, one of the main advantages of this device is the possibility to rotate the specimen without the need to refocus. The device enables easily reaching a precession deviation in the intersection point of axes up to 0.5 mm in the process of assembling.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Improved equipment; Microscopy; Mounted insects; Specimen manipulation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000100022

Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon
Provedor de dados: 63
Autores: Orce,Ingrid Georgina; Debes,Mario; Sendín,Lorena; Luque,Ana; Arias,Marta; Vojnov,Adrián; Marano,María; Castagnaro,Atilio; Filippone,María Paula.
ABSTRACT Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on the citrus industry. Extensive information is available about the disease but, nevertheless, the study of plant-pathogen interactions could provide new information in the understanding of citrus canker disease. A new isolate has been identified, Xcc AT, which has a high genetic similarity (&gt; 90 %) to the virulent Xcc T strain based on genetic clustering analyses of the rep-PCR fingerprinting patterns, but it does not produce cankerous lesions in Citrus limon. In this study, we compared C. limon responses to Xcc AT and to the virulent Xcc T strain at both histological and transcriptional levels. Histologically, leaves inoculated with Xcc AT...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA-AFLP; Gene expression; Canker; Plant defense; Microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000600552

Interspecific xenia and metaxenia in seeds and fruits of tomato
Provedor de dados: 63
Autores: Piotto,Fernando Angelo; Batagin-Piotto,Katherine Derlene; Almeida,Marcílio de; Oliveira,Giancarlo Conde Xavier.
Xenia, the transmission of traits from the pollinizer to the female's tissues, is a phenomenon hitherto unknown in tomatoes. Here, we describe xenia effects on the seeds and fruits of Solanum lycopersicum, the tomato, elicited by S. galapagense. The wild tomatoes, such as S. galapagense, have highly pilose fruit surface and minute seeds, unlike the domesticated species. Crossings between S. galapagense (pollinizer) and two large-seeded, glabrous cultivars of S. lycopersicum (females) tested the former's ability to raise the trichome density and trichome-to-1000-cell ratio and to reduce the seed weight in the latter's fruits. Selfed fruits of the two cultivars, Micro-Tom and Pusa Ruby, were compared to the crossed fruits. The pollen of S. galapagense was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hormones; Pollination; Microscopy; Wild relative.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200007

Photoperiod and feeding behavior of the Antarctic fish Notothenia rossii (Perciformes: Nototheniidae) and functional morphology of chemical and visual sensory structures used in foraging
Provedor de dados: 104
Autores: Donatti,Lucélia; Zaleski,Tânia; Calil,Patrícia; Fanta,Edith.
The influence of photoperiod on the ability of Notothenia rossii Richardson, 1844 to detect prey was studied experimentally. In 12L/12D there was the highest number of individuals stimulated visually and chemically while in 24D chemical perception was the same as that observed in 12L/12D, the latter being lower than in 24L. The ultrastructure of chemo- and photo-sensory structures, involved in the detection of food, was studied. The photoreceptor cells of the retina are single and double cones, and rods. Taste buds were concentrated in the central part of upper and lower lips. Their shape is even, but the size is variable. The olfactory rosette has a sensory and a non-sensory epithelium, being rich in different mucosubstances. The conclusion was that in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chemoreception; Microscopy; Photoreception.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752008000200014

Effects of salinity on the physiology of the red macroalga, Acanthophora spicifera (Rhodophyta, Ceramiales)
Provedor de dados: 39
Autores: Pereira,Débora Tomazi; Simioni,Carmen; Filipin,Elisa Poltronieri; Bouvie,Fernanda; Ramlov,Fernanda; Maraschin,Marcelo; Bouzon,Zenilda Laurita; Schmidt,Éder Carlos.
ABSTRACT Salinity is an important abiotic factor since it is responsible for the local and/or regional distribution of algae. In coastal regions, salinity changes with prevailing winds, precipitation and tide, and particularly in extreme intertidal conditions. Acanthophora spicifera is a red seaweed that occurs in the supratidal region in which changes in abiotic conditions occur frequently. This study evaluated the effects of salinity on the metabolism and morphology of A. spicifera. Algae were acclimatized under culture conditions with sterilized seawater for seven days. Experiments used different salinities (15 to 50 psu) for seven days, followed by metabolic analyses. This study demonstrates that extreme salinities affect physiological parameters of A....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acanthophora spicifera; Microscopy; Morphology; Physiology; Salinity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400555

Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators
Provedor de dados: 1
Autores: Santos, Dalilhia Nazaré dos; Nunes, Claudinéia Ferreira; Soares, Joyce Dória Rodrigues; Alves, Eduardo; Labory, Cláudia Regina Contijo; Pasqual, Moacir; Pio, Leila Aparecida Salles.
This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 µM 2,4-D; (3) 9.04 µM 2,4-D; (4) 4.14 µM PIC; (5) 8.28 µM PIC; (6) 4.52 µM 2,4-D + 2.32 µM KIN; (7) 9.04 µM 2,4-D + 4.64 µM KIN; (8) 4.14 µM PIC + 2.32 µM KIN; (9) 8.28 µM PIC + 4.64 µM KIN; (10) 4.52 µM 2,4-D + 2.22 µM BAP; (11) 9.04 µM 2,4-D + 4.44 µM BAP; (12) 4.14 µM PIC + 2.22 µM BAP and (13) 8.28 µM PIC + 4.44 µM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jatropha curcas L.; Embryogenesis; Microscopy; Cytochemistry.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19745

Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062
Provedor de dados: 1
Autores: Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras; Castro, Evaristo Mauro de; Universidade Federal de Lavras; Pereira, Fabricio José; Universidade Federal de Lavras; Braga, Francyane Tavares; Universidade Federal de Lavras.
Research on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical...
Palavras-chave: Microscopy; Orchidaceae; In vitro culture; Calcium silicate microscopy; Orchidaceae; In vitro culture; Calcium silicate.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062

Morfometria e histologia das gônadas de machos e fêmeas recém-eclodidos de Podocnemis expansa e Podocnemis unifilis (Testudines, Podocnemididae) - doi: 10.4025/actascibiolsci.v34i1.7257
Provedor de dados: 3
Autores: Malvasio, Adriana; Universidade Federal do Tocantins - UFT; Nascimento-Rocha, Josefa Moreira do; Universidade Federal do Tocantins - UFT; Santos, Helcileia Dias; Universidade Federal do Tocantins - UFT; Ataídes, Adson Gomes de; Universidade Federal do Tocantins - UFT; Portelinha, Thiago Costa Gonçalves; Universidade Federal do Tocantins - UFT.
P. expansa e P. unifilis. Todos os espécimes utilizados neste trabalho foram procedentes do rio Javaés, entorno do Parque Nacional do Araguaia, Estado do Tocantins, Brasil. Após estudo sob estereomicroscópio, foram coletadas as gônadas para o procedimento histológico de rotina. Foram analisadas 187 amostras de P. expansa, das quais 81,2% foram identificadas como fêmeas e 18,7% como machos; e 98 de P. unifilis, das quais 31,6% eram fêmeas e 68,4% machos. Não foram verificadas diferenças microscópicas relevantes na sexagem entre estas espécies. Os critérios utilizados no diagnóstico microscópico foram principalmente a morfologia das gônadas, a presença do apêndice no oviduto remanescente, a morfologia do oviduto e as características das células de...
Palavras-chave: 2.04.00.00-4 tartarugas; Sexagem; Ovário; Testículo; Microscopia; Tocantins 2.04.00.00-4 turtles; Sexing; Ovary; Testicle; Microscopy; Tocantins.
Ano: 2011
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7257

Comparative anatomy and histochemistry of the leaf blade of two species of Artocarpus
Provedor de dados: 42
Autores: SÁ,RAFAELA D.; CADENA,MARÍLIA B.; PADILHA,RAFAEL J.R.; ALVES,LUIZ CARLOS; RANDAU,KARINA P.
Abstract Abstract: In Brazil, there are two species of Artocarpus that were introduced: Artocarpus altilis (Parkinson) Fosberg, known as fruta-pão, and Artocarpus heterophyllus Lam., known as jaca. Both are used as food and medicine. The objective of this work was to conduct a comparative anatomical and histochemical study between A. altilis and A. heterophyllus. Techniques of optical, polarized and scanning electron coupled to energy dispersive spectroscopy. The anatomical characterization showed the characters of general occurrence in the family Moraceae and of those that allow the differentiation of A. altilis and A. heterophyllus. The histochemistry revealed the sites of synthesis and/or storage of the metabolites. The chemical microanalyses brought...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artocarpus altilis; Artocarpus heterophyllus; Crystals; Microscopy; Moraceae.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100851

Spray Drying of Pequi Pulp: Process Performance and Physicochemical and Nutritional Properties of the Powdered Pulp
Provedor de dados: 52
Autores: Santana,Audirene; Kurozawa,Louise; Oliveira,Rafael; Park,Kil.
The objective of this work was to optimize the spray drying of pequi pulp using maltodextrin as carrier agent and Tween 80 as surfactant agent. A central composite rotatable design was used to evaluate the influence of inlet air temperature (140 to 200°C), maltodextrin (15 to 30%) and surfactant (0 to 5%) concentration on the process performance and physicochemical and nutritional properties of the dried powdered pulp. The dependent variables were process yield (27.4 - 51.7%), outlet air temperature (106.5 - 135°C), energetic efficiency (29.9 - 44.8%), moisture content (0.25 - 1.43%), water activity (0.09 to 0.21), hygroscopicity (9.1 - 12.1 g adsorbed moisture/100g dry matter), vitamin C content (129.8 - 303.0 mg/g solids pequi) and total carotenoids...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fruit; Tween 80; Powder property; Screening design; Microscopy.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100500

Working Optimally with Serial Sections in Glycol Methacrylate Resin
Provedor de dados: 52
Autores: Leitão,Carlos André Espolador.
ABSTRACT The present work presents an optimized form for distributing serial sections in a glycol methacrylate slide set. This consists of filling the first row with sections from each slide, thus proceeding in the same way with the other rows. Therefore each slide will contain sections of three distinct sample segments.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytology; Histology; Historesin; Microscopy; Plant anatomy; Technique.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100416

Light microscope observation of circulating human lymphocytes cultured in vitro
Provedor de dados: 52
Autores: Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária.
The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013

Some segmental structural features of the aortic wall of domestic chicken (Gallus domesticus)
Provedor de dados: 62
Autores: Mello,Josiane Medeiros de; Orsi,Antonio Marcos; Padovani,Carlos Roberto; Matheus,Selma Maria Michelin; Torrejais,Márcia Miranda; Ribeiro,Antonio Augusto Coppi Maciel.
The segmental structure of the aortic wall at the thoracic and abdominal levels were studied in domestic chicken by light microscopy (LM) and scanning electron microscopy (SEM). Selected histological sections were submitted to histomorphometric studies using image analysis methods. The variables studied were the thickness of the aortic layers in the three segments investigated: ascendant thoracic, descendant thoracic and abdominal parts as well as the tubular diameters and the mean number of elastic lamellae in the medial layer of each analyzed segment. The aortic wall of the chicken had a predominately elastic structure in the thoracic portions, that decrease gradually in relative number of elastic lamellae towards the abdominal aorta in which smooth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aortic wall; Structure; Microscopy; Histophysiology; Domestic chicken.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000900001

Hepato-pulmonary amebiasis: a case report
Provedor de dados: 55
Autores: Shenoy,Vishnu Prasad; Vishwanath,Shashidhar; Indira,Bairy; Rodrigues,G.
Infections with Entamoeba histolytica are seen worldwide and are more prevalent in the tropics. About 90% of infections are asymptomatic, and the remaining 10% produce a spectrum of clinical syndromes, ranging from dysentery to abscesses of the liver or other organs. Extra-intestinal infection by E. histolytica most often involves liver. Pleuro-pulmonary involvement, seen as the second most common extra-intestinal pattern of infection, is frequently associated with amebic liver abscess. Pulmonary amebiasis occurs in about 2-3% of patients with invasive amebiasis. We report herein the case of a 45-year-old male presenting with hepato-pulmonary amebiasis. The diagnosis was established from direct examination of sputum, in which trophozoites of E. histolytica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entamoeba histolytica; Liver abscess; Microscopy; Pulmonary involvement.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400010

Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'
Provedor de dados: 58
Autores: Baldotto,Lílian Estrela Borges; Olivares,Fábio Lopes; Bressan-Smith,Ricardo.
The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Plant-growth promoting bacteria; Microscopy.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100015

Correlation between rheological properties and capillary fixation for modelling creams
Provedor de dados: 60
Autores: Demoliner,Lara Brogni; Agnol,Lucas Dall; Zanotti,Suzana Bittencourt; Dias,Fernanda Trindade Gonzalez; Angeli,Valeria Weiss; Bianchi,Otavio.
The Brazilian male beauty market occupies the second place in world consumption of cosmetics. Among the numerous products consumed by such audience is the capillary fixation mask, which is mainly composed by fixatives. These additives act on stabilizing the cosmetic emulsion, protecting the hair against moisture and also increasing the intensity of hair fixation. In this work, three formulations for modelling creams were prepared by different concentrations of the fixatives StylezeTM W20 and PVP K90 and their properties characterized by physicochemical, rheological and sensory analysis. The capillary masks produced were stable oil-in-water (O/W) emulsions with uniform droplets of 2.05-2.82 μm sizes and pseudoplastic thixotropic behavior (0.19 &lt; n &lt;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rheology; Microscopy; N-vinyl-2-pyrrolidone; Polyquaternium-55; Modelling cream/physicochemical properties; Cosmetics.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300621

Effect of Phytoaccumulation of Arsenic and Chromium on Structural and Ultrastructural Changes of Brake Fern (Pteris vittata)
Provedor de dados: 61
Autores: Sridhar,Balaji B. Maruthi; Han,Fengxiang X.; Diehl,Susan V.; Monts,David L.; Su,Yi.
Structural and ultrastructural changes caused by bioaccumulation of As and Cr in brake fern (Pteris vittata), a known arsenic hyperaccumulator, were investigated. Potted plants of brake fern were exposed to metal treatments of As and Cr for three weeks. Leaf, stem and root samples were collected periodically and fixed for LM (Light Microscopy), SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy) to evaluate anatomical changes. The fresh weights, dry weights, RWC (Relative Water Content) and plant heights were obtained before the brake fern plants were harvested for metal accumulation analysis. The As accumulated mainly in the shoots while Cr accumulated mainly in the roots of the metal-treated plants. Significant changes in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Arsenic; Chromium; Hyperaccumulator; Microscopy; Phytoremediation; Pteris vittata; Translocation; Ultrastructure.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000400006

Effects of Zn and Cd accumulation on structural and physiological characteristics of barley plants
Provedor de dados: 61
Autores: Sridhar,Balaji B. Maruthi; Han,Fengxiang X.; Diehl,Susan V.; Monts,David L.; Su,Yi.
The objectives of this study were to identify the structural changes caused by Zn and Cd accumulation in shoots and roots of barley (Hordeum vulgare) plants; and to correlate metal accumulation with anatomical, physiological and morphological changes. Potted plants were exposed to metal treatments of Zn and Cd for 19 and 16 d respectively. Leaves, stems and roots were harvested to identify structural changes and analyze metal accumulation. Barley effectively accumulated Zn (up to 11283 mg kg-1) and Cd (up to 584 mg kg-1) in the shoots. Microscopic structural changes, such as a decrease in intercellular spaces, breakdown of vascular bundles, and shrinkage of palisade and epidermal cells, occurred in leaves, stems and roots of plants treated with high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anatomy; Cadmium; Hordeum vulgare; Microscopy; Phytoremediation; Zinc.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100002

Granules morphology and rheological behavior of green banana (Musa cavendishii) and corn (Zea mays) starch gels
Provedor de dados: 64
Autores: Izidoro,Dayane Rosalyn; Demczuk Junior,Bogdan; Haminiuk,Charles Windson Isidoro; Sierakowski,Maria Rita; Freitas,Renato João Sossela de; Scheer,Agnes de Paula.
In this work, it was used starch obtained from green banana (Musa cavendishii) and commercial corn (Zea mays) starch in order to compare the granule morphology and the rheological behavior of these gel-starches. Images of starch granules morphology were obtained from scanning electron microscope (SEM). The banana starch granules presented an oval and ellipsoidal shape with irregular diameters. Neverthless, the granules of corn starch showed a poliedric shape, with different sizes. The rheological behavior of gel starch solutions showed a non-newtonian character with a pseudoplastic behavior. Herschel-Bulkley model gave a good description on the rheological behavior of the gel starch. Banana gel-starch solutions showed higher values of shear stress and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Starch; Green banana; Corn; Microscopy; Rheology.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000500026