Consulta: subjectFacets:"Molecular biology"
Registros recuperados: 59
Data/hora: 16/06/2024 00:51:19

Validation of Alternative Methods of Detection of Salmonella Spp. in Experimentally Contaminated Poultry Environmental Samples
Provedor de dados: 89
Autores: Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA.
ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303

Shannon index for estimating the diversity of nitrogen-fixing legume nodule bacteria using randomly amplified polymorphic DNA.
Provedor de dados: 119
Autores: MAIA, A. de H. N.; COUTINHO, H. L. C.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Biological nitrogen fixation; RAPD; Effects; Rhizobium; Azorhizobium; Bradyrhizobium; Environment; Measurement; Molecular biology; Soil bacteria; Statistics.
Ano: 1996
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/12544

Assessment of phenotypic indexes for aluminum tolerance in maize using nutrient solution.
Provedor de dados: 119
Autores: CANCADO. G. M. A.; MARTINS, P. R.; PARENTONI, S. N.; OLIVEIRA, A. B.; LOPES, M. A.
Tipo: Parte de livro
Palavras-chave: Tolerancia; RFLP; Aluminium; Tolerance; Savanna.; Alumínio; Biologia Molecular; Cerrado.; Molecular biology; Soil.
Ano: 1999
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/483530

Computational investigations in eukaryotes genome de novo assembly using short reads.
Provedor de dados: 119
Autores: CINTRA, L. C.
Recently news technologies in molecular biology enormously improved the sequencing data production, making it possible to generate billions of short reads totalizing gibabases of data per experiment. Prices for sequencing are decreasing rapidly and experiments that were impossible in the past because of costs are now being executed. Computational methodologies that were successfully used to solve the genome assembler problem with data obtained by the shotgun strategy, are now inefficient. Efforts are under way to develop new programs. At this moment, a stabilized condition for producing quality assembles is to use paired-end reads to virtually increase the length of reads, but there is a lot of controversy in other points. The works described in literature...
Tipo: Anais e Proceedings de eventos
Palavras-chave: Genomas de eucariotos; Bioinformática.; Biologia Molecular; Genoma.; Genome; Eukaryotic cells; Molecular biology; Bioinformatics.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/908741

Sex effect in gene expression of in vitro-produced bovine embryos vitrified by cryotop.
Provedor de dados: 119
Autores: LEME, L. O.; CARVALHO NETO, J. O.; DODE, M. A. N.
Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Abstracts.
Tipo: Separatas
Palavras-chave: Cryobiology; Diagnosis through imaging; Cryopreservation; Molecular biology.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1098800

Seleção para estresses abióticos como fator de redução de riscos na exploração agrícola: interação melhoramento de plantas, fisiologia vegetal e biologia molecular.
Provedor de dados: 119
Autores: PARENTONI, S. N.
Tipo: Parte de livro
Palavras-chave: Estresse; Abiotico; Plant.; Biologia Molecular; Fisiologia; Melhoramento; Planta; Stress.; Breeding; Physiology.; Molecular biology.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/485015

Biochemistry and molecular biology of N uptake and assimilation by plants.
Provedor de dados: 119
Autores: PURCINO, A. A. C.; ALVES, V. M. C.; PARENTONI, S. N.; MARRIEL, I. E.; SANTOS, F. G. dos; FRANCA, G. E.
Tipo: Parte de livro
Palavras-chave: Biologia Molecular; Bioquímica; Nitrogênio.; Biochemistry; Molecular biology; Nitrogen.
Ano: 1998
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/480862

Gene expression: a review on methods for the study of defense-related gene differential expression in plants.
Provedor de dados: 119
Autores: CASASSOLA, A.; BRAMMER, S. P.; CHAVES, M. S.; MARTINELLI, J. A.; GRANDO, M. F.; DENARDIN, N. D.
Tipo: Artigo de periódico
Palavras-chave: CDNA; Metabolic Routes; Plant Defense.; Gene expression; Molecular biology; Transcriptomics.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981342

Fingerprint molecular de porta-enxertos de citros via marcadores IRAP e Microssatélites.
Provedor de dados: 119
Autores: SOUZA JUNIOR, L. C. de; RAMOS, A. P. de S.; SOARES FILHO, W. dos S.; FERREIRA, C. F.
Objetivo: O objetivo desse trabalho foi estabelecer os prinicipais primers a serem usados para discriminar os PEs (porta-enxertos) mais utilizados do PMGC (Programa de Melhoramento Genético de Citros) da Embrapa em nível moleular usando marcadores SSR (Simple Sequence Repeats) e IRAP (Inter-Retrotransposon Amplified Polymorphism).
Tipo: Anais e Proceedings de eventos
Palavras-chave: Fruta Cítrica; Porta Enxerto; Marcador Molecular; Citrus; Rootstocks; DNA fingerprinting; DNA primers; Molecular biology.
Ano: 2021
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144777

Evaluation of the effectiveness of killer toxins produced by some strains of yeasts against other strains killer.
Provedor de dados: 119
Autores: CANOSSA, S.; AGUSTINI, B. C.; SILVA, G. A. da.
To make the wine, you do not need killer strains used for the purpose of dominance. It is only necessary that the yeasts are neutral. The aim of this study was to evaluate the effectiveness of killer toxins produced by some strains to produce a lethal effect on other strains killer. The experiments were conducted using the medium must Lorena M80: 20 with methylene blue. The killer test strains used were: 7M, 12M, 24M, 25M and 30M isolated from grape berries derived from the cultivar Merlot municipality of Pinto Bandeira (RS, Brazil). These strains were tested against other strains isolated killer Pinto Bandeira, they being the 3CF lineage derived from the cultivar Cabernet Franc, and the 24A strain isolated from cultivar Ancelota,; and also against strains...
Tipo: Separatas
Palavras-chave: Levedura.; Viticultura; Vinho; Fermento; Fermentação; Linhagem; Biologia molecular; Genética; Saccharomyces Cerevisiae.; Viticulture; Grapes; Genetics; Molecular biology; Yeasts.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1000945

Influence of crop rotation on microbial activity and yield in Cerrado agroecosystem.
Provedor de dados: 119
Autores: FERREIRA, P. S.; FABRÍCIO, A. C.; SILVA, V. B.; SCHLOTER, M.; LANGENBACH, T.
Tipo: Parte de livro
Palavras-chave: Brasil; Rotational cropping.; Biologia Molecular; Biologia do Solo; Cerrado; Rotação de Cultura.; Brazil; Molecular biology; Savannas; Soil biology.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/244453

Genome structure: uses of molecular tools.
Provedor de dados: 119
Autores: ZIMMERMANN, M. J. de O.
Editors: W. M. Roca; J. E. Mayer, M. A. Pastor-Corrales, J. M. Tohme.
Tipo: Parte de livro
Palavras-chave: Ferramenta; Feijão; Phaseolus Vulgaris; Planta; Marcador Molecular; Genome; Beans; Molecular biology.
Ano: 1994
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/195747

II curso de técnicas de biologia molecular aplicadas a produção animal
Provedor de dados: 119
Uso de marcadores moleculares na indústria animal; Introdução ao uso de marcadores animais Polimorfismo do sistema imune e sua aplicação em melhoramento para resistência a doenças; Métodos estatísticos para identificação de QTLs em animais; Estudo do desenvolvimento muscular em aves; Técnicas empregadas no estudo da expressão genicas; Animais trangenicos
Tipo: Livros
Palavras-chave: Industria animal; Desenvolvimento muscular; Animais trangenicos; Animal industry; Muscular; Biologia Molecular; Melhoramento; Breeding; Molecular biology.
Ano: 1999
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/45133

Influence of crop rotation on bacteria diversity and yield in Cerrado agroecossystem.
Provedor de dados: 119
Autores: FERREIRA, P. S.; FABRÍCIO, A. C.; SILVA, V. B.; SALTON, J. C.; SHLOTER, M.; LANGENBACH, T.
Tipo: Parte de livro
Palavras-chave: Brasil; Rotational cropping.; Biologia Molecular; Biologia do Solo; Cerrado; Rotação de Cultura.; Brazil; Molecular biology; Soil biology.; Savannas.
Ano: 2001
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/244452

Evaluation of MALDI-TOF mass spectrometry for identification of environmental yeasts and development of supplementary database.
Provedor de dados: 119
Autores: AGUSTINI, B. C.; SILVA, L. P. da; BLOCH JUNIOR, C.; BONFIM, T. M. B.; SILVA, G. A. da.
Yeast identification using traditional methods which employ morphological, physiological, and biochemical characteristics can be considered a hard task as it requires experienced microbiologists and a rigorous control in culture conditions that could implicate in different outcomes. Considering clinical or industrial applications, the fast and accurate identification of microorganisms is a crescent demand. Hence, molecular biology approaches has been extensively used and, more recently, protein profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has proved to be an even more efficient tool for taxonomic purposes. Nonetheless, concerning tomass spectrometry, data available for the differentiation of...
Tipo: Separatas
Palavras-chave: Levedura; MALDI-TOF MS; Microrganismo; Levedo; Biologia molecular; Base de dados; Genética; Yeasts; Molecular biology.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/997892

Opaque-2 modifiers: what they are and how they work.
Provedor de dados: 119
Autores: LARKINS, B. A.; DANNENHOFFER, J. M.; BOSTWICK, D. E.; OR, E.; MORO, G. A.; LOPES, M. A.
Tipo: Parte de livro
Palavras-chave: Milho opaco; Opaque-2; Protein; Quality; Biologia Molecular; Qualidade; Zea Mays; Molecular biology.
Ano: 1997
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/479110

Caracterização através da PCR de cepas de Bacillus thuringiensis de diferentes regiões do Brasil.
Provedor de dados: 119
Autores: VALICENTE, F. H.; VASCONCELOS, M. J. V.; PAIVA, E.; FARIA, A. L.; SOUZA, F. D.; BARRETO, M. R.
Tipo: Parte de livro
Palavras-chave: Brasil.; Biologia Molecular; Bacillus Thuringiensis; Solo.; Brazil; Molecular biology; Soil.
Ano: 1998
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/482991

A role in sugar and redox handling of maize aldose reductase.
Provedor de dados: 119
Autores: SOUSA, S. M. de; KIYOTA, E.; YUNES, J. A.; KOCH, K. E.
Tipo: Parte de livro
Palavras-chave: Biologia molecular; Molecular biology.
Ano: 2011
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/899387

Contribuição à filogenia de abelhas Melipona com uso de sequências parciais da região ITS1 do nrDNA.
Provedor de dados: 119
Autores: BOMFIM, I. G. A.; FREITAS, B. M.; VENTURIERI, G. C.; JORGE, D. M. de M.; LOBO, M. D. P.; TORRES, D. C.; GRANGEIRO, T. B.
O objetivo deste estudo foi investigar, por meio de dados moleculares de sequências de DNA, as relações filogenéticasde abelhas indígenas sem ferrão do táxon Melipona nativas do Brasil. Procurou-se, assim, fornecersubsídios para facilitar uma futura revisão taxonômica sobre esse grupo de abelhas. Sequências parciais daregião do espaçador transcrito interno I (ITS1) do DNA ribossômico nuclear (nrDNA) foram obtidas de espécimesde M. flavolineata, M. mondury e M. fasciculata, coletados em estados do Norte, Nordeste e Sudestedo Brasil. O alinhamento múltiplo das sequências geradas em conjunto com outras 25 do gênero Melipona,depositadas no GenBank ou publicadas em artigos científicos por outros autores, apresentou 141 caracteresalinhados, com uma distância...
Tipo: Separatas
Palavras-chave: Sequenciamento automático; Reconstrução filogenética; Abelhas sem ferrão; Meliponicultura; Automated sequencing; Phylogenetic reconstruction.; Biologia Molecular.; Molecular biology; Stingless bees.
Ano: 2014
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017063

Cultura dos citros.
Provedor de dados: 119
A história dos citros, no Brasil, teve início na Bahia e em São Paulo, onde jesuítas portugueses introduziram as primeiras sementes de laranja doce. Quatro séculos se passaram até que o País assumisse a liderança mundial na produção de citros. O Instituto Brasileiro de Geografia e Estatística (IBGE) revela que o valor total da produção brasileira de citros, compreendendo laranjas doces, tangerinas, limas ácidas e limões verdadeiros, atingiu, em 2010, a expressiva marca de R$ 7,1 bilhões, ocupando o quinto lugar entre as principais cadeias produtivas do agronegócio brasileiro. A sustentabilidade do agronegócio de citros depende, em grande parte, da adoção de tecnologias adequadas a essa finalidade, desenvolvidas à luz das reais necessidades nacionais, as...
Tipo: Livros
Palavras-chave: Biologia Molecular; Citogenética Vegetal; Citricultura; Fisiologia; Fruta Cítrica; Melhoramento Genético Vegetal; Porta Enxerto; Citrus fruits; Plant breeding; Molecular biology; Rootstocks.
Ano: 2013
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1157752

Using linear algebra for protein structural comparison and classification.
Provedor de dados: 119
Autores: GOMIDE, J.; MELO-MINARDI, R.; SANTOS, M. A. dos; NESHICH, G.; MEIRA JUNIOR, W.; LOPES, J. C.; SANTORO, M.
In this article, we describe a novel methodology to extract semantic characteristics from protein structures using linear algebra in order to compose structural signature vectors which may be used efficiently to compare and classify protein structures into fold families. These signatures are built from the pattern of hydrophobic intrachain interactions using Singular Value Decomposition (SVD) and Latent Semantic Indexing (LSI) techniques. Considering proteins as documents and contacts as terms, we have built a retrieval system which is able to find conserved contacts in samples of myoglobin fold family and to retrieve these proteins among proteins of varied folds with precision of up to 80%. The classifier is a web tool available at our laboratory website....
Tipo: Artigo de periódico
Palavras-chave: Protein classification; Contact maps; Linear algebra; Singular value decomposition; Latent semantic indexing; Biologia molecular; Molecular biology.
Ano: 2009
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/664383

Detecção do RNA genômico do vírus da artrite encefalite caprina (CAEV) por amplificação do gene estrutural GAG em amostras de leite.
Provedor de dados: 119
Autores: SOUZA, R. B.; OLIVEIRA, A. N. de; ANDRIOLI, A.; PINHEIRO, R. R.; SIDER, L. H.
Geral Desenvolver um método de diagnóstico molecular do vírus da artrite encefalite caprina (CAEV) por RT (Reverse Trancription)-nested PCR em amostras sangüíneas. Específicos Padronizar o método de extração de RNA das amostras realizado logo após a sua coleta ou congeladas a -20ºC e -80ºC. Padronizaras reações de síntese de cDNA e a amplificação de genes caprino e viral por RT-nested PCR.
Tipo: Anais e Proceedings de eventos
Palavras-chave: Artrite encefalite caprina; CAEV; RNA genômico; Caprino; Diagnostico; Goat diseases; Goats; Disease diagnosis; Caprine arthritis encephalitis virus; Molecular biology; RNA viruses.
Ano: 2008
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/529342

SCORPION VENOM: NEW PROMISE IN THE TREATMENT OF CANCER
Provedor de dados: 37
Autores: GÓMEZ RAVE,Lyz Jenny; MUÑOZ BRAVO,Adriana Ximena; SIERRA CASTRILLO,Jhoalmis; ROMÁN MARÍN,Laura Melisa; CORREDOR PEREIRA,Carlos.
ABSTRACT Cancer is a public health problem due to its high worldwide morbimortality. Current treatment protocols do not guarantee complete remission, which has prompted to search for new and more effective antitumoral compounds. Several substances exhibiting cytostatic and cytotoxic effects over cancer cells might contribute to the treatment of this pathology. Some studies indicate the presence of such substances in scorpion venom. In this review, we report characteristics of the principal scorpion venom components found in recent literature and their potential activity against tumor cells. There are different toxin groups present in the venom, and it seems that their mode of actions involves ionic channel blocking, disruption of the cell membrane...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-infective agents; Antineoplastic agents; Arachnida; Molecular biology; Peptides.
Ano: 2019
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000200213

Molecular heterogeneity of Malassezia pachydermatis through RAPD-PCR - doi: 10.4025/actascibiolsci.v32i2.4788
Provedor de dados: 3
Autores: Nascente, Patrícia da Silva; Universidade Federal de pelotas; Coimbra, Helen Silveira; Universidade Federal de Pelotas; Meinerz, Ana Raquel Mano; Universidade Federal de Pelotas; Meireles, Mário Carlos Araújo; Universidade Federal de Pelotas; Mello, João Roberto Braga; Universidade Federal do Rio Grande do Sul; Schuch, Luiz Filipe Damé; Universidade Federal de Pelotas.
Several methodologies in molecular biology have been used in the investigation of Malassezia pachydermatis and its differentiation into subtypes. Recent molecular research of this species includes the use of samples isolated from canine otitis externa and dermatitis, as well as from healthy animals, having in view an epidemiological study of the yeast. The aim of this study was to identify molecular differences in M. pachydermatis samples isolated from dogs with otitis externa. The M. pachydermatis strains were analyzed by means of the Random Amplification Primer DNA - Polimerase Chain Reaction (RAPD–PCR) for molecular heterogeneity research. DNA extraction was carried out with phenol-chloroform and the RAPD technique using the AGAATCCGCC primer. A...
Tipo: Estudo molecular
Palavras-chave: 2.00.00.00-6; 2.08.04.00-8; 2.12.00.00-9; 2.12.01.03-0 Malassezia pachydermatis; Molecular biology; Otitis externa; Dogs Ciências Biológicas; Biologia Molecular; Microbiologia; Micologia Malassezia pachydermatis; Molecular biology; Otitis externa; Dogs.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4788

Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell
Provedor de dados: 42
Autores: CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS.
ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599

Assessment of the scientific-technological production in molecular biology in Brazil (1996-2007): the contribution of genomics programs
Provedor de dados: 42
Autores: Meneghini,Rogério; Gamba,Estêvão C.
Several genome sequencing programs were launched in Brazil by the end of the nineties and the early 2000s.The most important initiatives were supported by the ONSA program (http://watson.fapesp.br/onsa/Genoma3.htm) and aimed at gaining domain in genomic technology and bringing molecular biology to the state of art. Two mainsets of data were collected in the 1996-2007 period to evaluate the results of these genome programs: the scientific production (Scopus and Web of Science databases) and the register of patents (US Patent and Trademark Office), both related to the progress of molecular biology along this period. In regard to the former, Brazil took a great leap in comparison to 17 other developed and developing countries, being only surpassed by China....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Science assessment; Genomics; Scientometrics; Molecular biology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200031

Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands
Provedor de dados: 45
Autores: Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A.
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013

Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
Provedor de dados: 52
Autores: Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de.
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019

Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET)
Provedor de dados: 52
Autores: Wiebe,Leonard I.
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Molecular imaging; PET; Nucleosides; Nucleotides; Nucleic acids; Oligonucleotides; Antisense; Aptamer; Spiegelmer.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300011

Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification
Provedor de dados: 52
Autores: Wu,Xulong; Xiao,Lu; Wang,Yin; Yang,Zexiao; Yao,Xueping; Peng,Bin.
ABSTRACT A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, sensitive and specific detection of African swine fever virus (ASFV). A set of LAMP primers was designed based on the sequence of the ASFV gene K205R. Reaction temperature and time were optimized to 64 oC and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or visually with the addition of fluorescent dye. The detection limit of the LAMP assay was approximately 6 copies of the target gene per microliter, 100 times more sensitive than conventional PCR. LAMP is a simple and inexpensive molecular assay format for ASFV detection. To date, African swine fever has not been reported in China. LAMP can be used to monitor ASFV spread into China,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: African swine fever virus; LAMP; K205R gene; Molecular biology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000200400

An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran
Provedor de dados: 55
Autores: Farahmand,Mahin; Nahrevanian,Hossein; Shirazi,Hasti Atashi; Naeimi,Sabah; Farzanehnejad,Zahra.
Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parasitological analysis; Molecular biology; Leishmaniasis; Cutaneous; Iran; Epidemiology.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004

Molecular biology of the kallikrein-kinin system: from structure to function
Provedor de dados: 56
Autores: Pesquero,J.B.; Bader,M.
The participation of the kallikrein-kinin system, comprising the serine proteases kallikreins, the protein substrates kininogens and the effective peptides kinins, in some pathological processes like hypertension and cardiovascular diseases is still a matter of controversy. The use of different experimental set-ups in concert with the development of potent and specific inhibitors and antagonists for the system has highlighted its importance but the results still lack conclusivity. Over the last few years, transgenic and gene-targeting technologies associated with molecular biology tools have provided specific information about the elusive role of the kallikrein-kinin system in the control of blood pressure and electrolyte homeostasis. cDNA and genomic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Kallikrein; Kinins; Bradykinin; Molecular biology; Transgenics.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900013

Molecular biology and clinical implication of hepatitis C virus
Provedor de dados: 56
Autores: Lyra,A.C.; Fan,X.; Di Bisceglie,A.M.
Hepatitis C virus (HCV) was first described in 1989 as the putative viral agent of non-A non-B hepatitis. It is a member of the Flaviviridae family and has been recognized as the major causative agent of chronic liver disease, including chronic active hepatitis, cirrhosis and hepatocellular carcinoma. HCV is a positive RNA virus with a genome containing approximately 9500 nucleotides. It has an open reading frame that encodes a large polyprotein of about 3000 amino acids and is characterized by extensive genetic diversity. HCV has been classified into at least 6 major genotypes with many subtypes and circulates within an infected individual as a number of closely related but distinct variants known as quasispecies. This article reviews aspects of the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hepatitis C; Molecular biology; Quasispecies.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500010

Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis
Provedor de dados: 56
Autores: Colombo,N.B.R.; Rangel,M.P.; Martins,V.; Hage,M.; Gelain,D.P.; Barbeiro,D.F.; Grisolia,C.K.; Parra,E.R.; Capelozzi,V.L.
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pequi; Caryocar brasiliense Camb; Oxidative stress; Lung cancer; Urethane; Molecular biology.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900852

p53 immunostaining is correlated with reduced survival and is not correlated with gene mutations in resected pulmonary large cell carcinomas
Provedor de dados: 56
Autores: Massoni Neto,L.M.; Bianchi,C.P.; Ab'Saber,A.M.; Parra,E.R.; Takagaki,T.; Pereira,J.C.; Soares,F.A.; Leite,K.; Capelozzi,V.L.
Malignancy of pulmonary large cell carcinomas (LCC) increases from classic LCC through LCC with neuroendocrine morphology (LCCNM) to large cell neuroendocrine carcinomas (LCNEC). However, the histological classification has sometimes proved to be difficult. Because the malignancy of LCC is highly dependent on proteins with functions in the cell cycle, DNA repair, and apoptosis, p53 has been targeted as a potentially useful biological marker. p53 mutations in lung cancers have been shown to result in expression and protein expression also occurs in the absence of mutations. To validate the importance of both p53 protein expression (by immunostaining) and p53 gene mutations in lung LCC (by PCR-single strand conformational polymorphism analysis of exons 5, 6,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Large cell carcinoma; P53; Genetics; Molecular biology; Lung cancer; Mutation analysis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800004

Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production
Provedor de dados: 58
Autores: Shi,Weifeng; Li,Kun; Ji,Yun; Jiang,Qinbo; Wang,Yuyue; Shi,Mei; Mi,Zuhuang.
Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin) genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR). The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the resistant isolates...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Klebsiella pneumoniae; AmpC β-Lactamase; Porin; Molecular biology.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200015

Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil
Provedor de dados: 58
Autores: Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton.
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113

MicroRNAs in veterinary cardiology
Provedor de dados: 65
Autores: Wolf,Marcela; Muehlbauer,Eloísa; Sousa,Marlos Gonçalves.
ABSTRACT: The use of biomarkers is an important recent development in veterinary medicine. Biomarkers allow non-invasive quantification of substances with diagnostic and prognostic potential in several diseases. The microRNAs are small, non-coding RNAs that regulate gene expression and are expressed in different forms in many diseases. Reduced or over-expression of microRNAs showed to be part of the pathogenesis of some heart diseases in humans and animals. Diagnostic and therapeutic value of measuring microRNAs in veterinary cardiology is increased because abnormal expression can be managed by the use of antagonists (in the case of overexpression) and mimicking (in the case of underexpression). Thus, this literature review aimed to compile scientific...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Genetic; Biomarker; Heart failure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700602

Multi-drug resistance gene (MDR1) and opioid analgesia in horses
Provedor de dados: 65
Autores: Natalini,Cláudio Corrêa; Cunha,Anderson Fávaro da; Linardi,Renata Lehn.
Opioid absorption in the intestinal tract as well as its effects in the central nervous system is modulated by the P-glycoprotein (P-gp) encoded in the Multi-drug Resistance gene (MDR1) also named ATP-binding cassete, subfamily B, member 1 (ABCB1). This MDR1 gene acts as a selective pump. The expression of this protein in humans and rodents inhibits cellular uptake of substrate opioids. The presence of the intestinal iso-enzyme CYP3A4 associated with MDR1 gene decreases the opioid analgesic activity due to an increase in intestinal metabolism, with a predicted intestinal first pass extraction around 20% which significantly influences the oral availability of opioids. In the central nervous system, P-gp expression decreases opioid neuronal uptake...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1; Analgesia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100055

Diagnostic methods for identification of root-knot nematodes species from Brazil
Provedor de dados: 65
Autores: Cunha,Tiago Garcia da; Visôtto,Liliane Evangelista; Lopes,Everaldo Antônio; Oliveira,Claúdio Marcelo Gonçalves; God,Pedro Ivo Vieira Good.
ABSTRACT: The accurate identification of root-knot nematode (RKN) species (Meloidogyne spp.) is essential for implementing management strategies. Methods based on the morphology of adults, isozymes phenotypes and DNA analysis can be used for the diagnosis of RKN. Traditionally, RKN species are identified by the analysis of the perineal patterns and esterase phenotypes. For both procedures, mature females are required. Over the last few decades, accurate and rapid molecular techniques have been validated for RKN diagnosis, including eggs, juveniles and adults as DNA sources. Here, we emphasized the methods used for diagnosis of RKN, including emerging molecular techniques, focusing on the major species reported in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Esterase; Meloidogyne; Molecular biology; Morphological pattern.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200153

Identification of multi-drug resistance gene (MDR1) in equine ileum
Provedor de dados: 65
Autores: Natalini,Cláudio Corrêa; Linardi,Renata Lehn.
P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048

Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
Provedor de dados: 65
Autores: Mega,Nicolás Oliveira; Revers,Luís Fernando.
Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arthropod DNA; High quality DNA; Molecular biology; AFLP.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012

Microbial diversity of soils on the banks of the Solimões and Negro rivers, state of Amazonas, Brazil
Provedor de dados: 74
Autores: Santos,Ellen Karla Nobre dos; Honda,Rubens Tomio; Nozawa,Sérgio Ricardo; Ferreira-Nozawa,Monica Stropa.
Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region. Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices. The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this. The interaction between the nucleotide sequences of bacteria inhabiting the two riverine environments indicated that the two microrganism communities are similar in composition.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbiological diversity; Tropical soil biodiversity; 16S rDNA gene; Amazon; Molecular biology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100019

Morphological, enzymatic and molecular characterization of root-knot nematodes parasitizing vegetable crops
Provedor de dados: 75
Autores: Barros,Aline F; Campos,Vicente P; Souza,Larissa N; Costa,Sarah S; Terra,Willian C; Lessa,Josimar HL.
ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Meloidogyne; Diagnosis; Molecular biology; Vegetable crops.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473

Extração de DNA e obtenção de marcadores moleculares para diferentes espécies de interesse para o cerrado.
Provedor de dados: 14
Autores: BELLON, G.; FALEIRO, F. G.; BARROS, A. M.; KARIA, C. T.; ANDRADE, R. P.; CORDEIRO, M. C. R.; PINTO, A. C.; JUNQUEIRA, N. T. V.; PEREIRA, A. V.; PEREIRA, E. B. C.; FERNANDES, F. D.; FERREIRA, M. E.
Tipo: Folders
Palavras-chave: Planta nativa; Indigenous organisms; Biotecnologia; Biologia Molecular; Cerrado; DNA; Marcador Molecular; Marcador Genético; Genetic markers; Biotechnology; Molecular biology.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/553057

Operacionalizacao da extracao de DNA de especies nativas do cerrado visando analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
Tipo: Folders
Palavras-chave: Planta nativa; Indigenous organisms; Biologia Molecular; Biotecnologia; Cerrado; DNA; Marcador Genético; Marcador Molecular; Biotechnology; Genetic markers; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567935

Caracterizacao molecular e estabelecimento de colecao de trabalho de Stylosanthes macrocephala com base em marcadores RAPD.
Provedor de dados: 14
Autores: FALEIRO, F. G.; BARROS, A. M.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.; GOMES, A. C.; ANDRADE, R. P.
Tipo: Folders
Palavras-chave: RAPD; Feed legumes; Biologia Molecular; Biotecnologia; Marcador Molecular; Leguminosa Forrageira; Variação Genética; Genetic variation; Biotechnology; Genetic markers; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567912

Biologia molecular aplicada à diagnose de doenças de plantas.
Provedor de dados: 14
Autores: GONCALVES, R. C.
As doenças das plantas influenciam negativamente e de modo significativo as atividades agropecuárias e florestais em todo o mundo. Muitas epidemias tiveram como conseqüência grandes perdas econômicas, alterações sociais e ambientais.
Tipo: Parte de livro
Palavras-chave: Marcadores genéticos; Enfermedades y desórdenes de las plantas; Doença de planta; Marcador genético; Biologia Molecular; Plant diseases and disorders; Molecular biology; Genetic markers.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/661798

Identificação molecular e caracterização da diversidae de espécies de fungos micorrízicos arbusculares do gênero Gigaspora por PCR-DGGE (Denaturing Gradient Gel Electrophoresis).
Provedor de dados: 14
Autores: DE SOUZA, F. A.
Metodologia. estirpes de fungos MA avaliadas. Amostras de solo. Extração e preparação de esporos para análise de DNA. Experimento teste de PCR para verificar a especificidade dos "primers" para a família Gigasporaceae. Experimento de casa-de-vegetação. Amplificação de DNA de esporos utilizando um PCR em "nested". PCR utilizando primers específicos para análise de comunidades de fungos MA da família Gigasporaceae. Análise . T. S. C. Análise de DGGE. Teste de reprodutibilidade. Seqüenciamento de DNA a apartir da recuperação de DNA de bandas de géis de DGGE. Clonagem do rDNA de fungos MA. Seleção de clones via PCR-DGGE e seqüenciamento de DNA. Alinhamento de seqüências. Número de acesso no EMBL das seqüências de nucleotídeos obtidas neste projeto. Resultados....
Tipo: Livros
Palavras-chave: Biologia Molecular; Micorriza Vesicular Arbuscular; Molecular biology; Vesicular arbuscular mycorrhizae.
Ano: 2005
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/626859

Eutils-search versão 2.0 - manual do usuário.
Provedor de dados: 14
Autores: TANAKA, R. S.; HIGA, R. H.
O software Eutils-search tem por objetivo trazer do banco de dados PubMed informações sobre artigos relacionados a genes de um organismo específico, de acordo com as regras referentes à taxa de acesso impostas pelo site. As informações trazidas são, então, armazenadas localmente em um banco de dados para acesso rápido. Além disso, o software também gera documentos XML correspondentes às informações do organismo requisitado. O eutils-search é uma ferramenta de apoio ao desenvolvimento de aplicações de mineração de textos voltadas para os domínios de biotecnologia e biologia molecular, baseada em informações textuais obtidas do banco de dados PubMed. Este documento apresenta os pré-requisitos e a descrição dos parâmetros necessários para utilização do...
Tipo: Folhetos
Palavras-chave: Software eutils-search; Mineração de texto; Text mining; Software; Biotecnologia; Biologia Molecular; Biotechnology; Molecular biology.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/920856

Uso de ferramentas moleculares em estudos da diversidade de microrganismos do solo.
Provedor de dados: 14
Autores: REIS JUNIOR, F. B. dos; MENDES, I. de C.; TEIXEIRA, K. R. dos S.; REIS, V. M.
CRI6557
Tipo: Folhetos
Palavras-chave: Microbiologia do Solo; Manejo do Solo; Biologia Molecular; Biotecnologia; Polimorfismo; População Microbiana; Molecular biology; Soil; Soil management.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/559150

Diagnóstico molecular para o nematóide Rotylenchulus reniformis.
Provedor de dados: 14
Autores: CORDEIRO, M. C. R.; GOULART, A. M. C.; COSTA, A. M.; SHARMA, R. D.
Tipo: Folhetos
Palavras-chave: Biologia molecular; Nematóide; Rotylenchulus reniformis; Molecular biology; Nematoda.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571879

Uso de marcadores RAPD e do sistema de informacao geografica no estudo da variabilidade genetica de Stylosanthes macrocephala.
Provedor de dados: 14
Autores: BARROS, A. M.; FALEIRO, F. G.; SHIRATSUCHI, L. S.; KARIA, C. T.; LOPES, G. K. B.; ANDRADE, R. P.
Tipo: Folders
Palavras-chave: RAPD; Stylosanthes macrocepala; Feed legumes; Biogeografia; Biotecnologia; Marcador Molecular; Leguminosa Forrageira; Marcador Genético; Variação Genética; Sistema de Informação Geográfica; Genetic markers; Geographic information systems; Biogeography; Biotechnology; Genetic variation; Molecular biology.
Ano: 2004
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567934

Aplicação da biologia molecular em programas de conservação de recursos pesqueiros.
Provedor de dados: 14
Autores: MARQUES, D. K. S.
Estudos genéticos em peixes; Marcadores moleculares; Eletroforese de proteínas; Seqüências de DNA como marcadores; Polimorfismo de comprimento de fragmentos de restrição (Restriction Fragment Length Polymorphism - RFLP); Polimorfismo de DNA amplificado ao acaso (Randomly Aplified polymorphic DNA - RAPD); DNA com seqüências repetitivas: micro-satélites e mini-satélites; DNA mitocondrial.
Tipo: Folhetos
Palavras-chave: Marcadores moleculares; Recursos pesqueiros; Fisheries resources; Conservation; Biologia Molecular; Conservação; Peixe; População; Fish; Molecular biology; Population.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810724

A biologia avançada e o impacto da genômica na produção de caprinos e ovinos.
Provedor de dados: 14
Autores: SIDER, L. H.; ZAROS, L. G.
Tipo: Folhetos
Palavras-chave: Genômica; Caprino; Ovino; Biologia molecular; Goats; Sheep; Molecular biology; Genomics.
Ano: 2008
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/534121

Biologia molecular aplicada à sanidade.
Provedor de dados: 14
Autores: SIDER, L. H.
Tipo: Artigo na mídia
Palavras-chave: Biologia molecuar; Sanidade; Molecular biology.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/577884

Metodologia para operacionalizar a extracao de DNA de especies nativas do cerrado visando a analises moleculares.
Provedor de dados: 14
Autores: FALEIRO, F. G.; FALEIRO, A. S. G.; CORDEIRO, M. C. R.; KARIA, C. T.
Tipo: Folhetos
Palavras-chave: Biotecnologia; Biologia Molecular; Cerrado; DNA; Engenharia Genética; Marcador Molecular; Marcador Genético; Melhoramento Genético Vegetal; Genetic markers; Genetic engineering; Biotechnology; Plant breeding; Molecular biology.
Ano: 2003
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/559088

Etude des facteurs de transcription impliqués dans l’accumulation lipidique en condition de stress azoté chez la microalgue haptophyte Isochrysis affinis galbana
Provedor de dados: 5
Autores: Thiriet-rupert, Stanislas.
In every organism, evolution and acclimation to environmental changes are orchestrated by numerous molecular players. Among them, transcription factors (TFs) play a crucial role by regulating gene expression. Therefore, identify TFs involved in the production of high value products is a significant step in a biotechnological context. The laboratory has at its disposal a mutant strain of the haptophyte microalga Tisochrysis lutea producing twice more storage lipids than the wild type strain when exposed to nitrogen deprivation. Given the key role of TFs in phenotype establishment, this PhD aim at identify the TFs involved in that of the mutant phenotype of T. lutea. A TFs identification and classification pipeline was elaborated and applied to T. lutea’s...
Tipo: Text
Palavras-chave: Bioinformatique; Biologie moléculaire; Évolution; Facteur de transcription; Micro-algue; Réseau de gènes; RNA-seq; Bioinformatic; Evolution; Gene network; Molecular biology; Microalgae; RNAseq; Transcription factor.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00371/48233/48368.pdf

Efecto de la levadura Saccharomyces  cereviciaie desintegrada y tres fracciones en una línea celular y como aditivo alimentario en el cultivo de Artemia
Provedor de dados: 20
Autores: Comabella Soto, Y.; Centro de Investigaciones Marinas Universidad de La Habana.
En el presente estudio se evalúa el efecto que la levadura  Saccharomyces cerevisiae de forma  desintegrada (LD) y tres fracciones obtenidas de ella (complejo pared-membrana (FP), núcleo- proteínas (FN) y citoplasma (FC)) produce en dos modelos biológicos experimentales: la línea celular  MB16-F10 y la especie Artemia franciscana. Para los estudios celulares se empleó la parte soluble de  cada una de estas fracciones probándose las concentraciones de 0.5, 1, 2, 5 y 10  µg/mL  adicionándose el valor de 25 µg/mL para LD y FC. Se determinó el efecto en la proliferación, vitalidad  y fases del ciclo celular a las 48 y 72 horas. Como resultado se obtuvo una acción estimulante de la  proliferación celular con LD 5 µg/mL a las 48 horas  y con FN 0.5 y 1 µg/mL a...
Tipo: Theses and Dissertations
Palavras-chave: Molecular biology; Cell biology.
Ano: 2003
URL: http://hdl.handle.net/1834/5666