Consulta: subjectFacets:"Molecular identification"
Registros recuperados: 27
Data/hora: 14/06/2024 02:45:21

Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping
Provedor de dados: 63
Autores: Clasen,Bárbara Estevão; Silveira,Andressa de Oliveira; Baldoni,Daiana Bortoluzzi; Montagner,Daiane Fiuza; Jacques,Rodrigo Josemar Seminoti; Antoniolli,Zaida Inês.
ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: PCR; Ectomycorrhizal fungi; Molecular identification; Morphotype.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential.
Provedor de dados: 119
Autores: MAIA, N. da C.; SOUZA, P. N. da C.; GODINHO, B. T. V.; MOREIRA, S. I.; ABREU, L. M. de; JANK, L.; CARDOSO, P. G.
This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited...
Tipo: Artigo de periódico
Palavras-chave: Antagonism; Molecular identification; RDNA; Grasses; Plant pathogens.
Ano: 2018
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110371

Genetic diversity of the root-knot nematode Meloidogyne enterolobii and development of a SCAR marker for this guava-damaging species.
Provedor de dados: 119
Autores: TIGANO, M. S.; SIQUEIRA, K. de; CASTAGNONE-SERENO, P.; MULET, K.; QUEIROZ, P. R.; SANTOS, M. DOS; TEIXEIRA, C.; ALMEIDA, M. R. A.; CARNEIRO, R. M. D. G.
Tipo: Separatas
Palavras-chave: Molecular identification; RAPD; ISSR; Psidium Guajava.
Ano: 2010
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/872608

Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul1.
Provedor de dados: 119
Autores: CAZOLA, D de O.; JORGE, K. dos S. G.; ZUMÁRRAGA, M. J.; SOUZA-FILHO, A. F.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.
Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping.
Tipo: Artigo de periódico
Palavras-chave: Molecular identification; Poligotyping.; Mycobacterium Bovis.; Bovine tuberculosis; Molecular epidemiology.
Ano: 2015
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1015208

New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum.
Provedor de dados: 119
Autores: MARQUES, E.; MARTINS, I.; CUNHA, M. de O. C.; LIMA, M. A.; SILVA, J. B. T. da; SILVA, J. P. da; INGLIS, P. W.; MELLO, S. C. M.
Tipo: Separatas
Palavras-chave: Recursos genéticos microbiano; Identificação molecular; Biocontrole; Molecular identification; Biocontrol; White mold.; Mofo branco.; Microbial genetic resources.
Ano: 2016
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058264

Diversity of yeasts during fermentation of cocoa from two sites in the Brazilian Amazon
Provedor de dados: 36
Autores: ALMEIDA,Silvana de F. Oliveira de; SILVA,Letícia R Carvalho; JUNIOR,Gilson Celso A. Chagas; OLIVEIRA,Guilherme; SILVA,Silvia Helena Marques da; VASCONCELOS,Santelmo; LOPES,Alessandra Santos.
ABSTRACT The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicilândia and Tucumã), State of Pará, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicilândia and 29 from Tucumã. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicilândia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Accession; Molecular identification; Cocoa beans; Pichia Manshurica; Saccharomyces cerevisiae; Theobroma cacao.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100064

Dominant yeasts associated to mango (Mangifera indica) and rose apple (Syzygium malaccense) fruit pulps investigated by culture-based methods
Provedor de dados: 42
Autores: RAMÍREZ-CASTRILLÓN,MAURICIO; USMAN,LUISA M.; SILVA-BEDOYA,LINA M.; OSORIO-CADAVID,ESTEBAN.
Abstract: The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domain of the large ribosomal RNA gene (LSU rRNA gene) or internal transcribed spacer (ITS) 1, ribosomal gene 5.8S and ITS 2 (ITS) regions of one to several...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mangifera indica; Syzygium malaccense; Molecular identification; Yeasts; Colombia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700626

Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Provedor de dados: 42
Autores: PIE,MARCIO R.; STRÖHER,PATRÍCIA R.; AGOSTINIS,ANDRÉ O.; BELMONTE-LOPES,RICARDO; TADRA-SFEIR,MICHELLE Z.; OSTRENSKY,ANTONIO.
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: EDNA; Invasive species; Environmental DNA; Molecular identification.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041

Culture and molecular identification of microorganisms from Digital Dermatitis lesions in dairy cattle: Leptospira, an unexpected finding
Provedor de dados: 45
Autores: Diniz,S.; Sandes,S.H.C.; Bomfim,M.R.Q.; Santos,P.C.; Cruz,F.D.; Moreira,T.F.; Carvalho,M.R.S.; Cisalpino,P.S.; Moreira,E.S. A.
ABSTRACT Bovine digital dermatitis (BDD) is an infectious and contagious disease characterized by ulcerative and proliferative lesions affecting the skin on the bulbs of the heel or the interdigital cleft in dairy cattle, often associated with lameness. Evidences on the etiology of BDD indicate that it is multifactorial, involving environmental factors and multiple bacterial colonization. We isolated and identified microorganisms from BDD biopsy samples obtained from five Holstein Friesian and two Jersey cows by cultivation and molecular identification of bacterial isolates using 16S rRNA gene sequence analysis. We identified six bacterial species: Spirochetes as Treponema pedis and Leptospira broomi/L. fainei, L. licerasiae/L. wolffii; Corynebacterium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine digital dermatitis; Molecular identification; 16S rRNA gene analysis; Treponema; Leptospira.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300559

New isolates of Trichoderma antagonistic to Sclerotinia sclerotiorum
Provedor de dados: 49
Autores: Marques,Eder; Martins,Irene; Cunha,Mariana de Oliveira Cardoso; Lima,Marcello Arrais; Silva,João Batista Tavares da; Silva,Joseane Padilha da; Inglis,Peter Ward; Mello,Sueli Correa Marques.
Abstract Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microbial genetic resources; Molecular identification; Biocontrol; White mold.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032016000300209

An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans
Provedor de dados: 52
Autores: Fungaro,Maria Helena Pelegrinelli; Sartori,Daniele.
Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins. Its occurrence in several agricultural commodities has been considered a worldwide problem. This toxin is mainly produced by Aspergillus species. OTA has nephrotoxic, immunosuppressive, and carcinogenic effects and consequently the contamination with this toxin represents a high risk for human and animal health. In the last 5 years, several investigators have applied molecular methods in order to develop PCR assays for identifying and quantifying OTA-producing fungi in coffee beans samples. The main objective is to allow the detection of microorganisms capable of producing OTA, preferentially prior to ochratoxin production and accumulation. In this contribution several of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Molecular marker; PCR detection; Molecular identification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700001

Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR
Provedor de dados: 52
Autores: Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454

Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil
Provedor de dados: 52
Autores: Reddy,Kondakindi Venkateswar; Vijayalashmi,Tatiparti; Ranjit,Pabbati; Raju,Maddela Naga.
ABSTRACT The wide variety of bacteria in the environment permits screening for more efficient cellulases to help overcome current challenges in biofuels production. This study focuses on the isolation of efficient cellulase producing bacteria found in pulp and paper mill effluent contaminated soil which can be considered for use in large scale biorefining. Four different bacterial strains were isolated and screened for cellulase production by using CMC agar medium. All isolates showed cellulase activity these strains were further characterized by morphological, physiological, biochemical and 16S rRNA gene analyses. These isolates were identified as two Bacillus subtilis sub sps, Bacillus mojavensis and Bacillus cereus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CMC Agar; Cellulolytic Bacteria; Molecular identification; 16S rRNA; Cellulolytic activity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100442

A new PCR approach for the identification of Fusarium graminearum
Provedor de dados: 58
Autores: Biazio,Gleison Ricardo de; Leite,Gabriela Guimarães Sousa; Tessmann,Dauri José; Barbosa-Tessmann,Ione Parra.
The main objective of this work was to develop a PCR protocol for the identification of Fusarium graminearum, based on a pair of primers targeted to a segment of the 3' coding region of the gaoA gene that codes for the enzyme galactose oxidase (GO). This region has low homology with the same region of GO genes from other fungi. Genomic DNA from 17 strains of Fusarium spp. isolated from diseased cereals, from several other Fusarium species, and from other fungi genera was analyzed in a PCR assay using this primer set. The 17 strains of Fusarium spp. were also analyzed for the GO enzyme production in submerse fermentation in a new formulated liquid medium. All strains that were morphologically and molecularly identified as F. graminearum were able to secrete...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusarium graminearum; Galactose oxidase; GaoA gene; Molecular identification.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300028

Yeast diversity associated to sediments and water from two Colombian artificial lakes
Provedor de dados: 58
Autores: Silva-Bedoya,L.M.; Ramírez-Castrillón,M.; Osorio-Cadavid,E.
In Colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. Sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. The main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in Universidad del Valle (Cali-Colombia). Yeast samplings were performed from fifteen sediment samples and ten water samples. Grouping of similar isolates was initially based on colony and cell morphology, which was then complemented by micro/mini satellite primed PCR banding...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yeast diversity; Artificial lake; Molecular identification; MSP-PCR fingerprinting.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100019

Molecular prevalence and factors associated with Babesia vogeli infection in dogs in the Cerrado Mato-Grossense region of Brazil
Provedor de dados: 65
Autores: Castro,Vanessa Vieira; Ayres,Eveline da Cruz Boa Sorte; Canei,Darlan Henrique; Pereira,Mariana Elisa; Sousa,Valéria Régia Franco; Chitarra,Cristiane Silva; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de.
ABSTRACT: Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p&gt;0.05), greater positivity was observed in dogs&lt;1 year of age, male sex, those with free...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Babesiosis; Molecular identification; Cuiaba.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200500

Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions
Provedor de dados: 65
Autores: Oliveira,Camilla Martins de; Souza Junior,Ismail Teodoro; Araújo Filho,Jerônimo Vieira de; Freitas,Marcos Augusto de; Rocha,Mara Rúbia da; Petrofeza,Silvana.
ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Root-knot nematode; Glycine max; Esterase; Molecular identification.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500151

Evaluation of the genetic diversity among maize lines (Zea mays L.) by DNA fingerprinting analysis
Provedor de dados: 65
Autores: Echeverrigaray,Sergio; Delamare,Ana Paula Longaray; Tosello,Geraldo; Gal,Orit; Hillel,Jossi; Lavi,Uri.
Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimate the genetic distances between them. The relationship obtained by DNA fingerprinting analysis of the eight inbreed lines was highly consistem with the ir genetical origin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular identification; Genetic distances; Zea mays.
Ano: 1996
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000300028

Validation of the multiplex PCR for identification of  Brucella spp.
Provedor de dados: 65
Autores: Orzil,Lívia de Lima; Preis,Ingred Sales; Almeida,Iassudara Garcia de; Souza,Patrícia Gomes de; Soares Filho,Paulo Martins; Jacinto,Fabrício Barcelos; Fonseca Júnior,Antônio Augusto.
ABSTRACT: A multiplex PCR technique for detection of  Brucella spp. in samples of bacterial suspension was validated as a complementary tool in the diagnosis of the disease. This technique allows the characterization of the agent without performing biochemical tests, which greatly reduces the time for a final diagnosis, and provides more security for the analyst by reducing the time of exposure to microorganisms. The validation was performed in accordance with the Manual of Diagnostic Tests from OIE (2008) and following the requirements present in the ABNT NBR ISO/IEC 17025:2005. The mPCR validated in this study identified the different species of Brucella ( Brucella abortus ,  B. suis , B. ovis e B. melitensis ) of bacterial suspension obtained from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brucellosis; Molecular identification; Zoonosis; Brucella; Multiplex PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500847

Infección cruzada de especies de Colletotrichum en frutos tropicales en postcosecha.
Provedor de dados: 32
Autores: Aguilar Pérez, Luis Alfonso.
El género Colletotrichum es considerado uno de los principales hongos patógenos de plantas, este hongo se encuentra distribuido a escala mundial y afecta a diferentes cultivos tanto en pre y postcosecha. En el presente trabajo se aislaron e identificaron morfológicamente y molecularmente ocho aislamientos de Colletotrichum sp, de banano, papaya, carambolo, chile manzano, aguacate, mango ataulfo y mango manila, estos se inocularon sobre cada uno de los frutos para evaluar el fenómeno de infección cruzada. Se midió diariamente el diámetro de la lesión por siete días. Los resultados mostraron que algunos aislamientos de C. gloeosporioides son más agresivos cuando se inoculan sobre su hospedero original, sin embargo ocasionan daños en menor grado en hospederos...
Palavras-chave: Colletotrichum; Frutos tropicales; Identificación molecular; Infección cruzada; Tropical fruits; Molecular identification; Cross infection; Maestría; Fitopatología.
Ano: 2010
URL: http://hdl.handle.net/10521/306

Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil
Provedor de dados: 74
Autores: Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031

Identificação molecular de fitoplasma do grupo 16Srlll: agente causal de superbrotamento de mandioca (2009).
Provedor de dados: 14
Autores: ISOTON, F. T.; RABELLO, A. R.; OLIVEIRA, A. S. de; BATISTA, B. C. de C.; RESENDE, R. de O.; SILVA, M. S.
Tipo: Folders
Palavras-chave: Manihot esculenta Crantz; Fitoplasma; Phytoplasmas; Molecular identification; Mandioca; Cassava.
Ano: 2009
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/747802

Ticks (Acari: Ixodidae) infesting cattle and some other domestic and wild hosts on the French Mediterranean island of Corsica
Provedor de dados: 5
Autores: Grech-angelini, Sebastien; Stachurski, Frederic; Lancelot, Renaud; Boissier, Jerome; Allienne, Jean-francois; Marco, Sylvain; Maestrini, Oscar; Uilenberg, Gerrit.
Background: Corsica is a mountainous French island in the north-western Mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. Despite a favourable context for ticks and tick-borne diseases (TBDs), the tick fauna of Corsica has not systematically been investigated. Methods: For one year (May 2014-May 2015), a survey of ticks infesting cattle was performed in the three Corsican cattle slaughterhouses. Two of these were visited monthly and one quarterly; the ticks were manually collected, just after flaying. Ticks were identified on their morphology; when necessary, some specimens were also molecularly identified by sequencing mitochondrial cox1 (cytochrome c oxidase subunit...
Tipo: Text
Palavras-chave: Ticks (Ixodidae); Cattle; Domestic animals; Wild animals; Molecular identification; Corsica.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00361/47191/71863.pdf

Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters
Provedor de dados: 5
Autores: Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.
Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...
Tipo: Text
Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf

Rediscovery of Aquilaria rostrata (Thymelaeaceae), a species thought to be extinct, and notes on Aquilaria conservation in Peninsular Malaysia
Provedor de dados: 16
Autores: Lee, S.Y.; Mohamed, R.
 After more than 100 years since the first discovery, Aquilaria rostrata, a critically endangered species listed in the IUCN Red List and presumably extinct, has been rediscovered in Terengganu State of Peninsular Malaysia. Here, we describe the history, taxonomy, ecology and conservation status of this endemic species, and compare our findings with the species description made from the first and only collection produced prior to this study. In addition, we present the similarities between A. rostrata and several Aquilaria species occurring in Peninsular Malaysia and neighbouring regions using molecular sequence data from the nuclear ribosomal DNA (Internal Transcribed Spacer) and chloroplast intergenic spacer region (trnL-trnF). Our morphological and...
Tipo: Article / Letter to the editor
Palavras-chave: Aquilaria; Conservation status; Molecular identification; Peninsular Malaysia; Single mutations; Taxonomy; 42.48.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/606307

Molecular assessment of Gymnotus spp. (Gymnotiformes: Gymnotidae) fishing used as live baitfish in the Tietê River, Brazil
Provedor de dados: 83
Autores: Faria-Pereira,Lilian Paula; Hilsdorf,Alexandre Wagner Silva; Albert,James; Paiva,Maria José Tavares Ranzani; Galvão,Márcia Santos Nunes.
ABSTRACT The capture of live bait for sport fishing is an important activity for fishing communities. The main species used for this purpose are members of the genus Gymnotus, which comprises numerous species of cryptic nature that are difficult to identify based on external morphology. The aims of this work were to identify through partial sequences of the COI gene Gymnotus species fished in the Jacaré-Guaçu River, SP, and to develop a molecular diagnostic approach using PCR-RFLP to identify these species. Partial COI sequences were compared to those of other species deposited in GenBank. The sequences were assessed in the NEBCutter program to determine restriction sites in the sequence and the enzymes to be tested. Phenetic analysis performed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular identification; PCR-RFLP; Tuvira.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500205

Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential
Provedor de dados: 96
Autores: Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303