Consulta: subjectFacets:"Molecular marker"
Registros recuperados: 57
Data/hora: 20/09/2024 21:21:26

QTL IDENTIFICATION FOR CASSAVA BACTERIAL BLIGHT RESISTANCE UNDER NATURAL INFECTION CONDITIONS
Provedor de dados: 37
Autores: SOTO SEDANO,Johana; MORA MORENO,Rubén Eduardo; CALLE,Fernando; LÓPEZ CARRASCAL,Camilo Ernesto.
Cassava, Manihot esculenta Crantz, represents the main food source for more than one billion people. Cassava's production is affected by several diseases, one of the most serious is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). A quantitative trait loci (QTL) analysis for CBB resistance was performed under natural infection conditions, using a mapping population of 99 full-sibs genotypes highly segregant and a SNP-based high dense genetic map. The phenotypic evaluation was carried out in Puerto López, Meta, Colombia, during the rainy season in 2015. Both resistant and susceptible transgressive segregants were detected in the mapping population. Through a non-parametric interval mapping analysis, two QTL were detected,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cassava; Molecular marker; QTL; Resistance; SNPs; Xanthomonas axonopodis pv. manihotis.
Ano: 2017
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100002

Development and characterization of microsatellite loci for Campomanesia xanthocarpa (Myrtaceae) and cross amplification in related species
Provedor de dados: 3
Autores: Góes, Bruna Delgado; Ruas, Eduardo Augusto; Benício, Lucas Milanez; Cassiano, Daniele; Souza, Felipe Pinheiro de; Ruas, Paulo Maurício.
Campomanesia xanthocarpa is a native tree, of common occurrence in almost all Brazilian Forest formations, which has its fruits and timber with high commercial value. Using an enriched genomic library we isolated and characterized microsatellite loci for C. xanthocarpa (Myrtaceae), in order to estimate genetic diversity parameters for this and related species. Twenty-eight microsatellite loci were identified and ten of them successfully amplified and showed polymorphism in a sample of 96 individuals, from four natural populations. The number of alleles per locus ranged from two to eight, and the observed and expected heterozygosities varied from 0.042 to 1.000 and from 0.294 to 0.855, respectively. These markers were tested and validated in two related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências Biológicas gabiroba; Genetic diversity; Molecular marker; Native tree; Simple sequence repeat (SSR) Desenvolvimento de Marcadores Simple Sequence Repeats.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43454

Genetic variability and diversity of mutant rice revealed by quantitative traits and molecular markers
Provedor de dados: 115
Autores: Oladosu,Yusuff; Rafii,Mohd Y.; Abdullah,Norhani; Malek,Mohammad Abdul; Rahim,Harun A.; Hussin,Ghazali; Ismail,Mohd Razi; Latif,Mohammad Abdul; Kareem,Isiaka.
Genetic variability with mutagenic agents has been employed in plant breeding due to its significant contribution to the improvement of the existing rice genotypes by using genetically diverse parents. This study evaluated the genetic variability and diversity of mutant rice using quantitative traits and inter-simple sequence repeats (ISSR) molecular markers. A field experiment was carried out on M5 generation of 31 developed mutant lines and their parent (control). Morphological traits and 25 ISSR molecular markers were used as tools for determining cultivar identities and genetic diversity. The selected primers generated 443 clear polymorphic bands with an average number of 18 per primer. The bands were 85.10 % polymorphic. Un-weighted Pair Group of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Genetic diversity; Molecular marker; Mutagenicity; Polymerase chain reaction (PCR).
Ano: 2015
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000300002

Genetic diversity of the sunflower caterpillar (Chlosyne lacinia saundersii Doubleday and Hewitson) (Lepidoptera: Nymphalidae) populations determined by molecular RAPD markers
Provedor de dados: 42
Autores: Cunha,Fabiane; Gómez,Daniel R. Sosa; Silva,Jose J. da; Alexandre,Talita M.; Moscardi,Flávio.
Chlosyne lacinia saundersii is one of the most important pests of sunflower and it is the main target of insecticides applications. Larvae were collected in Londrina (PR), Santa Maria (RS), Dourados (MS), Ribeirão Preto (SP), Brasília (DF), Barreiras (BA), Uberaba (MG) and Vilhena (RO). Genomic DNA was extracted and amplified with ten-mer primers, which produced 101 loci. The size of the RAPD amplicons ranged from 180 to 2564 bp. Polymorphism among populations ranged from 31% to 67%, with the highest polymorphisms of 57% and 67% being detected in Uberaba and Vilhena populations, respectively. Populations with the highest similarity determined with Dice coefficient were from Ribeirão Preto and Barreiras, while insects from Londrina showed the highest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amova; Gene flow; Molecular marker; Sunflower pest.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400029

Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs
Provedor de dados: 45
Autores: Figueiredo,F.C.; Lopes,P.S.; Pinto,A.P.G.; Paiva,D.A.F.; Mendonça,P.T.; Guimarães,S.E.F.
The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P&lt;0.05), jowl (P&lt;0.05), sirloin (P&lt;0.10), and kidneys (P&lt;0.01). These results showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Swine; Candidate gene; Molecular marker; DNA polymorphism; DNA sequencing.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200020

Quantitative trait loci mapping for meat quality traits in swine chromosome 6
Provedor de dados: 45
Autores: Pires,A.V.; Lopes,P.S.; Guimarães,S.E.F.; Guimarães,C.T.; Gomide,L.A.M.; Benevenuto Júnior,A.A.; Carmo,F.M.S.
The current study was carried out to perform QTL mapping on swine chromosome 6 (SSC6) associated to meat quality traits. The F2 population was produced by outbreed crossing using two native Brazilian breed Piau boars and 18 commercial sows. A total of 557 F2 animals were genotyped for 13 microsatellite markers. The traits evaluated on the F2 population were: pH measured 45 minutes and 24 hours post mortem (pH 45, pH24, respectively), drip loss (DL), cooking loss (CL), total loss (TL), intramuscular fat content (IMF), objective tenderness (OT), lightness (L), redness (A), yellowness (B), hue angle (h) and chrome (c). Data were analyzed by multiple regression developed for analysis of outbreed line crosses, using the QTL Express Software. Significant QTL...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: QTL; Outbreed cross; Genetic; Molecular marker; Animal breeding.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500006

The pioneering use of ISSR (Inter Simple Sequence Repeat) in Neotropical anurans: preliminary assessment of genetic diversity in populations of Physalaemus cuvieri (Amphibia, Leiuperidae)
Provedor de dados: 48
Autores: Moresco,Rafaela M; Maniglia,Thiago C; De Oliveira,Classius; Margarido,Vladimir P.
The greatest diversity of anurans in the world is in Brazil and one of the major challenges is to reconcile the accelerated economic development with strategies that aim to maintain this diversity in forest fragments, often representing ESUs of some biomes. This study aimed to obtain data that will support conservation projects through the pioneering use of ISSR analysis in Neotropical anurans, estimating the intra- and interpopulation genetic diversity of four populations of P. cuvieri (Paraná and São Paulo regions). Of the 65 loci scored 58 were polymorphic, with 0.797 intrapopulation variation and 0.203 interpopulation variation. The index of interpopulation genetic differentiation (Fst) proved to be high among the population of Marmeleiro-PR and the...
Tipo: Journal article
Palavras-chave: ESU; Gene flow; Genetic structure; Molecular marker.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100008

An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans
Provedor de dados: 52
Autores: Fungaro,Maria Helena Pelegrinelli; Sartori,Daniele.
Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins. Its occurrence in several agricultural commodities has been considered a worldwide problem. This toxin is mainly produced by Aspergillus species. OTA has nephrotoxic, immunosuppressive, and carcinogenic effects and consequently the contamination with this toxin represents a high risk for human and animal health. In the last 5 years, several investigators have applied molecular methods in order to develop PCR assays for identifying and quantifying OTA-producing fungi in coffee beans samples. The main objective is to allow the detection of microorganisms capable of producing OTA, preferentially prior to ochratoxin production and accumulation. In this contribution several of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Molecular marker; PCR detection; Molecular identification.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700001

Genetic analysis of species in the Genus Catasetum (ORCHIDACEAE) using RAPD Markers
Provedor de dados: 52
Autores: Oliveira,Luciana do Valle Rego; Faria,Ricardo Tadeu de; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Santos,Melissa de Oliveira; Carvalho,Valdemar P.
In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catasetum; Molecular marker; Orchid; RAPD; Genetic similarity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200017

Varietal identification of coffee seeds by RAPD technique
Provedor de dados: 52
Autores: Crochemore,Maria Lúcia; Nunes,Liliane Moreira; Andrade,Giselly Aparecida; Molinari,Hugo Bruno Correa; Vasconcellos,Maria Elizabeth.
This study aimed the identification of cultivars and/or lines of Coffea arabica of commercial interest, using PCR-RAPD markers. The DNA of ground seeds lots of 12 cultivars and/or lines were evaluated with five primers (Operon OPA 01, OPA 04, OPG 11, OPY 16, and OPX 09) were obtained from a selection of 56 primers. The electrophoretic profiles allowed distinction among eight cultivars and/or lines as well as heterogeneity between and within lots of IAPAR59.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; Cultivar identification; Molecular marker.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100002

Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer
Provedor de dados: 52
Autores: Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza.
ABSTRACT  Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features.  Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software.  Results: HER2 and ESR1 overexpression was identified in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100304

Gene Expression Analyses of HER-2/neu and ESR1 in Patients with Breast Cancer
Provedor de dados: 52
Autores: Nadergoli,Omid Kheyri; Feizi,Mohammad Ali Hosseinpour; Kafil,Hossein Samadi; Pouladi,Nasser; Hosseinzadeh,Ali; Rostamizadeh,Leila; Montazeri,Vahid; Fakhrjou,Ashraf; Sakhinia,Ebrahim; Seifi,Morteza.
ABSTRACT Background: Her-2 and ESR1 genes, that interact in the cell signaling pathway, are the most important molecular markers of breast cancer, which have been amplified or overexpressed in 30% and 70%, respectively. This study was performed to evaluate the gene expression levels of Her-2 and ESR1 genes in tumor cells and its adjacent normal tissue of breast cancer patients and compared them whit clinical-pathological features. Methods: In total, 80 tissue specimens from 40 patients, with an average age of 48.47 years, were examined by Real-time PCR technique, and ultimately evaluated the expression level of Her-2 and ESR1genes. The data were analyzed by REST 2009 V2.0.13 statistical software. Results: HER2 and ESR1 overexpression was identified in 19...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breast Cancer; Her-2/neu; ESR1; Overexpression; Molecular marker; Real-time PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100317

Association between the c.910A&amp;gt;G genetic variant of the XRCC1 gene and susceptibility to esophageal cancer in the Chinese Han population
Provedor de dados: 56
Autores: Chen,X.Q.; Wang,F.; Zheng,Y.L.; Fan,Q.X.; Yue,D.L.; Ma,Z.J.
Esophageal cancer (EC) is a common malignancy worldwide. The X-ray repair cross-complementing 1 gene (XRCC1) is one of the most important candidate genes for influencing susceptibility to EC. This study aimed to investigate the effect of XRCC1 genetic variants on susceptibility to EC. A total of 383 EC patients (males: 239, females: 144, mean age: 56.62) and 387 cancer-free controls (males: 251, females: 136, mean age: 58.23) were enrolled in this study. The c.910A&gt;G genetic variant of theXRCC1 gene was determined by polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods. The allele and genotype frequencies indicated statistical differences between EC patients and cancer-free controls. The c.910A&gt;G genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Esophageal cancer; XRCC1 gene; Genetic variant; Molecular marker; Susceptibility.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001201028

A conservative region of the mercuric reductase gene (merA) as a molecular marker of bacterial mercury resistance
Provedor de dados: 58
Autores: Sotero-Martins,Adriana; Jesus,Michele Silva de; Lacerda,Michele; Moreira,Josino Costa; Filgueiras,Ana Luzia Lauria; Barrocas,Paulo Rubens Guimarães.
The most common bacterial mercury resistance mechanism is based on the reduction of Hg(II) to Hg0, which is dependent of the mercuric reductase enzyme (MerA) activity. The use of a 431 bp fragment of a conservative region of the mercuric reductase (merA) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MerA gene; Mercury resistant; Bacteria; Molecular marker.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200020

Genetic variation within three populations of Phycella australis (Phil.) Ravenna from Biobío Region, Chile, evaluated using ISSR markers
Provedor de dados: 117
Autores: Flores,Cristian; Delaveau,Catherine; Alvarez,Carolina; Vergara,Dafne; Baeza,Carlos; Ruiz,Eduardo; Uribe,Matilde.
Phycella australis (Phil.) Ravenna is a Chilean plant with high ornamental potential; however, the intensive extraction as a cut flower might be detrimental for the conservational state by ignoring the state of genetic variation. The objective of this investigation was to assess genetic variability within and between three populations of P. australis in the Biobío Region using inter-simple sequence repeat (ISSR) markers. The evaluated areas correspond to three locations in the province of Concepción, Biobío Region: Desembocadura (36°48' S, 73°10' W), Santa Juana (36°58' S, 72°58' W), and Lipinhue (37°00' S, 72°58' W). Six ISSR primers were used obtaining 51 fragments, from which 72.5% were polymorphic. From the three evaluated sites Santa Juana showed a...
Tipo: Journal article
Palavras-chave: Amaryllidaceae; Genetic variability; Molecular marker; Ornamental plant.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100012

Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
Provedor de dados: 69
Autores: Neeraja,Chirravuri Naga; Vemireddy,Lakshminarayana Reddy; Malathi,Surapaneni; Siddiq,Ebrahimali Abubacker.
After the success of IR8 and TN1, breeders depended heavily on these two rice cultivars for source of short stature led to the narrow genetic base to majority of present day rice varieties, as far as sd1 (semi-dwarf1) gene is concerned. In addition, analysis of genetic lineage of the majority of the cultivated rice varieties in tropical Asia reveals that sd1 from DGWG (Dee-Gee-Woo-Gen) is the major source of dwarfing gene. Such high amount of genetic homogeneity renders rice plants vulnerable to epidemic of diseases and insect pests. In the current study, we made an attempt to identify the alternate sources of DGWG allele of sd1 gene by characterizing 29 induced and 3 spontaneous dwarf accessions employing marker for DGWG allele of sd1 gene and exogenous...
Tipo: Journal article
Palavras-chave: Dee-Gee-Woo-Gen; Dwarfing gene; Gibberellic acid assay; Molecular marker; Rice; Sd1 gene.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300007

Population structure and genetic diversity of Brazilian popcorn germplasm inferred by microsatellite markers
Provedor de dados: 69
Autores: da Silva,Tereza Aparecida; Belizário Cantagalli,Liriana; Saavedra,Javier; Lopes,Ana Daniela; Aparecida Mangolin,Claudete; Pires da Silva Machado,Maria de Fatima; Scapim,Carlos Alberto.
Background The genetic diversity and structure of 31 popcorn accessions of the germplasm bank of the State University of Maringá were assessed using 30 microsatellite primers. Results 127 alleles were identified from 30 evaluated loci. The number of alleles per locus ranged from two to eight. The overall mean of the polymorphic loci averaged 79.89%. The primers UMC1549 and UMC1072 detected polymorphism in all accessions analyzed. The mean observed heterozygosity ranged from 0.07 to 0.30 and the highest proportion of heterozygous plants was observed in accession BOZM 260 (Ho = 0.30). The analysis of molecular variance revealed that 60% of the total genetic variation was found within accessions and 40% was found between accessions. The Bayesian clustering...
Tipo: Journal article
Palavras-chave: Genetic variability; Maize; Molecular marker.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300007

Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05.10 revealed by genomic sequence
Provedor de dados: 69
Autores: Zhao,Ming; Zhou,Jin yan; Li,Zhi dong; Song,Wei wei; Tan,You jiu; Tan,Hong.
Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They...
Tipo: Journal article
Palavras-chave: Botrytis cinerea; LTR retrotransposons; Molecular marker; Plant pathogen; Population diversity; Transposable elements.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300002

In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations
Provedor de dados: 74
Autores: Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui.
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013

Association between G316A growth hormone polymorphism and economic traits in pigs
Provedor de dados: 74
Autores: Faria,Danielle Assis de; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Paiva,Samuel Rezende; Sollero,Bruna Pena; Wenceslau,Amauri Arias.
The association between G316A growth hormone polymorphism and quantitative traits was investigated in an F2 population of pigs. Association analyses were performed using a statistical model that included genotype, sex, batch and sex by genotype interaction as fixed effects and sire as random effect. The polymorphism was associated with the number of right teats (p = 0.03), heart weight (p = 0.04), lung weight (p = 0.05), carcass length determined by the Brazilian carcass classification method (p = 0.04), picnic shoulder weight (p = 0.07), jowl weight (p = 0.01), pH 24 h after slaughtering (p = 0.03) and drip loss (p = 0.01). Interaction between genotype and sex was observed for six performance traits. The additive effect was significant (p &lt; 0.10) for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Candidate genes; Divergent cross; Molecular marker; Sex x genotype interaction; Swine.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400010

Microsatellite markers: what they mean and why they are so useful
Provedor de dados: 74
Autores: Vieira,Maria Lucia Carneiro; Santini,Luciane; Diniz,Augusto Lima; Munhoz,Carla de Freitas.
Abstract Microsatellites or Single Sequence Repeats (SSRs) are extensively employed in plant genetics studies, using both low and high throughput genotyping approaches. Motivated by the importance of these sequences over the last decades this review aims to address some theoretical aspects of SSRs, including definition, characterization and biological function. The methodologies for the development of SSR loci, genotyping and their applications as molecular markers are also reviewed. Finally, two data surveys are presented. The first was conducted using the main database of Web of Science, prospecting for articles published over the period from 2010 to 2015, resulting in approximately 930 records. The second survey was focused on papers that aimed at SSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR biological function; Genomic distribution; Genotyping approaches; Molecular marker; Practical utility.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300312

A molecular method for the detection of sally lightfoot crab larvae (Grapsus grapsus, Brachyura, Grapsidae) in plankton samples
Provedor de dados: 74
Autores: Ströher,Patrícia R.; Firkowski,Carina R.; Freire,Andrea S.; Pie,Marcio R.
The decapod Grapsus grapsus is commonly found on oceanic islands of the Pacific and Atlantic coasts of the Americas. In this study, a simple, quick and reliable method for detecting its larvae in plankton samples is described, which makes it ideal for large-scale studies of larval dispersal patterns in the species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dispersal; Aratu; Sayapa; Molecular marker.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300026

Development of two sequence-specific PCR markers linked to the le gene that reduces pod shattering in narrow-leafed Lupin (Lupinus angustifolius L.)
Provedor de dados: 74
Autores: Boersma,Jeffrey G.; Buirchell,Bevan J.; Sivasithamparam,Krishnapillai; Yang,Huaan.
Wild types of narrow-leaf lupin (Lupinus angustifolius L.) have seed pods that shatter upon maturity, leading to the loss of their seeds before or during the harvest process. Two recessive genes have been incorporated into domesticated cultivars of this species to maximize harvest-ability of the produce. One of these genes is called lentus (le). Two microsatellite - anchored fragment length polymorphism (MFLP) candidate markers were identified as closely linked to the le gene in a recombinant inbred line (RIL) population derived from a domesticated x wild type cross. The candidate MFLP markers were isolated from the gel, re-amplified by PCR, cloned and sequenced. The MFLP polymorphisms were converted into sequence-specific PCR-based markers. Linkage...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pod shattering; Lupin; MFLP; Molecular marker; Marker assisted selection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400020

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)
Provedor de dados: 74
Autores: Sosa-Gómez,Daniel R.
The velvetbean caterpillar (VBC), Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae), is one of the most important New World soybean agro-ecosystems pests, occurring from 40° N in the USA to 39° S in Argentina. Information on the migration patterns of the VBC moth may be important for managing the resistance of VBC populations to insecticides or plants carrying the Bacillus thuringiensis insecticide genes, especially since sedentary populations have a higher potential to became resistant than migratory populations. We studied intraspecific variations of geographically distinct VBC populations in order to determine the genetic distance between them and to assess the variability of VBC populations from near the city of Londrina (Paraná (PR)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular marker; RAPD; Gene flow; Soybean pest; Population structure.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300012

Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley
Provedor de dados: 74
Autores: Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar.
Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021

Comparative proteomic analysis of indica and japonica rice varieties
Provedor de dados: 74
Autores: Yang,Yanhua; Zhu,Keming; Xia,Hengchuan; Chen,Liang; Chen,Keping.
Indica and japonica are two main subspecies of Asian cultivated rice (Oryza sativa L.) that differ clearly in morphological and agronomic traits, in physiological and biochemical characteristics and in their genomic structure. However, the proteins and genes responsible for these differences remain poorly characterized. In this study, proteomic tools, including two-dimensional electrophoresis and mass spectrometry, were used to globally identify proteins that differed between two sequenced rice varieties (93-11 and Nipponbare). In all, 47 proteins that differed significantly between 93-11 and Nipponbare were identified using mass spectrometry and database searches. Interestingly, seven proteins were expressed only in Nipponbare and one protein was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Indica and japonica rice; Molecular marker; Proteomics; Quantitative real-time PCR; Unique proteins.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500008

Development of microsatellite markers for identifying Brazilian Coffea arabica varieties
Provedor de dados: 74
Autores: Vieira,Elisa S.N.; Von Pinho,Édila V. de R.; Carvalho,Maria G.G.; Esselink,Danny G.; Vosman,Ben.
Microsatellite markers, also known as SSRs (Simple Sequence Repeats), have proved to be excellent tools for identifying variety and determining genetic relationships. A set of 127 SSR markers was used to analyze genetic similarity in twenty five Coffea arabica varieties. These were composed of nineteen commercially important Brazilians and six interspecific hybrids of Coffea arabica, Coffea canephora and Coffea liberica. The set used comprised 52 newly developed SSR markers derived from microsatellite enriched libraries, 56 designed on the basis of coffee SSR sequences available from public databases, 6 already published, and 13 universal chloroplast microsatellite markers. Only 22 were polymorphic, these detecting 2-7 alleles per marker, an average of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR; Coffee; Genetic similarity; Molecular marker.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300022

Identification of genes related to high royal jelly production in the honey bee (Apis mellifera) using microarray analysis
Provedor de dados: 74
Autores: Nie,Hongyi; Liu,Xiaoyan; Pan,Jiao; Li,Wenfeng; Li,Zhiguo; Zhang,Shaowu; Chen,Shenglu; Miao,Xiaoqing; Zheng,Nenggan; Su,Songkun.
Abstract China is the largest royal jelly producer and exporter in the world, and high royal jelly-yielding strains have been bred in the country for approximately three decades. However, information on the molecular mechanism underlying high royal jelly production is scarce. Here, a cDNA microarray was used to screen and identify differentially expressed genes (DEGs) to obtain an overview on the changes in gene expression levels between high and low royal jelly producing bees. We developed a honey bee gene chip that covered 11,689 genes, and this chip was hybridised with cDNA generated from RNA isolated from heads of nursing bees. A total of 369 DEGs were identified between high and low royal jelly producing bees. Amongst these DEGs, 201 (54.47%) genes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Honeybee; Royal jelly production; Gene chip; Molecular marker; Differentially expressed genes.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500781

Mating system of a population of Myracrodruon urundeuva F.F. &amp;amp; M.F. Allemão using the fAFLP molecular marker
Provedor de dados: 74
Autores: Freitas,Miguel L.M.; Sebbenn,Alexandre M.; Moraes,Mario L.T.; Lemos,Eliana G.M.
The mating system and genetic diversity were studied in a natural population of Myracrodruon urundeuva originating from 30 open-pollinated trees at the Paulo de Faria Ecological Station, SP, Brazil. The progenies were planted on the Teaching and Research Farm of the Ilha Solteira Engineering School, UNESP. Using the fAFLP molecular marker, eleven loci were selected to study the mating system. The mating system was analyzed using the multilocus mixed-mating model. The estimates of genetic divergence between pollen and ovule allele frequencies were significant for eight loci, suggesting nonrandom outcrossing. The estimates of the multilocus outcrossing rate revealed that M. urundeuva possesses a mating system with a predominance of outcrossing events (theta...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myracrodruon urundeuva; Mating system; FAFLP; Molecular marker; Correlation of paternity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300019

The association of CAPN1 316 marker genotypes with growth and meat quality traits of steers finished on pasture
Provedor de dados: 74
Autores: Miquel,María C.; Villarreal,Edgardo; Mezzadra,Carlos; Melucci,Lilia; Soria,Liliana; Corva,Pablo; Schor,Alejandro.
The objective of this paper was to determine the association of a SNP in the µ-calpain gene at position 316 with growth and quality of meat traits of steers grown on pasture. Fifty-nine Brangus and 20 Angus steers were genotyped for CAPN1 316. Warner Bratzler shear force was measured in l. lumborum samples after a 7-day aging period. A multivariate analysis of variance was performed, including shear force (WBSF), final weight (FW), average daily gain (ADG), backfat thickness (BFT), average monthly fat thickness gain (AMFTG), rib-eye area (REA), and beef rib-eye depth (RED) as dependent variables. The CAPN1 316 genotype was statistically significant. Univariate analyses were done with these variables. The marker genotype was statistically significant (p...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Tenderness; Growth; Molecular marker; Multivariate analysis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300011

Association between MUC1 gene polymorphism and expected progeny differences in Nelore cattle (Bos primigenius indicus)
Provedor de dados: 74
Autores: Souza,Fabio Ricardo Pablos de; Vozzi,Pedro Alejandro; Vila,Reginaldo Aparecido; Boligon,Arione Augusti; Galerani,Marli Aparecida Vani; Lobo,Raysildo Barbosa; Martelli,Lucia Regina.
MUC1 is a heavily glycosylated mammalian transmembrane protein expressed by mucosal secretory tissues for both protection against microbial infection and lubrication. An important characteristic of MUC1 is its variable number of tandem repeats (VNTR) containing several sites for O-glycosylation. VNTR length has been associated with many human diseases and with certain economically important traits in domestic ruminants. The aim of the present study was to correlate the length of MUC1 gene VNTR with expected progeny differences (EPDs) obtained for growth, fertility and carcass traits. Five alleles were identified, with alleles containing short VNTRs being more frequent than those with long, thereby demonstrating that Brazilian Nelore cattle are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Molecular marker; Productive traits; Quantitative trait locus.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100013

Variabilidade genética de acessos de trigo com base em marcadores moleculares RAPD (2007).
Provedor de dados: 14
Autores: RIBEIRO JÚNIOR, W. Q.; FALEIRO, F. G.; BELLON, G.; COSTA, A. M.; CORDEIRO, M. C. R.; ALBRECHT, J. C.; CASTRO, R. L. de; BRAMMER, S. P.; SOESILVA, M.
Tipo: Folders
Palavras-chave: Melhoramento genético; Molecular marker; Genetic variability; Marcador Molecular; Trigo; Variação Genética; Wheat.
Ano: 2007
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/571574

Impact of aquaculture on the genetic structure of Mediterranean populations of Dicentrarchus
Provedor de dados: 5
Autores: Bahri Sfar, Lilia; Lemaire, Christophe; Chatain, Beatrice; Divanach, Pascal; Ben Hassine, Oum Kalthoum; Bonhomme, Francois.
A phylogeographic analysis based on 6 polymorphic microsatellites was performed on 15 sea bass samples. five from Western Mediterranean, seven from Eastern Mediterranean and three coming from French aquaculture stocks. Among the eastern samples, three did not cluster according to their geographic origin but rather with the occidental group. Furthermore. a somewhat lower allelic diversity was observed within these particular samples, indicating that they originated from a limited number of progenitors of foreign origin. Among the aquaculture stocks, only one revealed a significant reduction of genetic variability, indicating that these stocks are largely outbred and open to wild fishes. The fact that the use of occidental fingerlings to seed broodstocks in...
Tipo: Text
Palavras-chave: Dicentrarchus labrax; Farmed populations; Escapees; Molecular marker.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-410.pdf

Characterization and genetic relationships among Brazilian biotypes of Schizaphis graminum (Rondani) (Hemiptera: Aphididae) using RAPD markers
Provedor de dados: 82
Autores: Lopes-da-Silva,Marcelo; Tonet,Gabriela E.L.; Vieira,Luiz G.E.
The greenbug Schizaphis graminum (Rondani) is one of the most important cereals pests in the world. Within populations of this species, several biotypes, which are clones that share same virulence relationships with cultivated plants, can be distinguished. Molecular markers have been used to genetically characterize insect populations because they are fast and cost effective. In order to find RAPD markers to identify Brazilian S. graminum biotypes, nineteen clonal populations of three biotypes (B, C and E) from Brazil and three clonal populations from the U.S. were examined. Eighteen primers were used to analyze the material, but only six primers revealed polymorphisms and among those, three produced diagnostic band profiles that allowed biotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; Molecular marker; Greenbug; Insect-plant relationship.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000100009

RAPD variation within and among species of Melittobia Westwood (Hymenoptera: Eulophidae)
Provedor de dados: 82
Autores: Sari,Eloisa H.R.; Tavares,Mara G.; Campos,Lucio A.O.
The genus Melittobia Westwood comprises several species of microparasitoids and only two of them are know to occur in Brazil up to now: M. australica Girault and M. hawaiiensis Perkins. Nevertheless, the differentiation between these two species using traditional taxonomy is very difficult. In the present study, we used random amplified polymorphic DNA chain reaction (RAPD-PCR) to test for its ability to discriminate between these two species and to examine the genetic variation among the studied populations of M. australica. Most of the generated fragments were species-specific, occurring in all individuals of one species and absent in the individuals of the other species demonstrating the appropriateness of such technique to distinguish between both of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Melittobia australica; M. hawaiiensis; Molecular marker; Genetic variability; Microparasitoid.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000200007

Genetic similarity among Agistemus pallinii Matioli et al (Acari: Stigmaeidae) found in citrus orchards in Viçosa, Minas Gerais State, Brazil
Provedor de dados: 82
Autores: Matioli,André L; Pallini,Angelo; Tavares,Mara G.
Stigmaeidae are very important predators of mite and insect pests on several crops in Brazil. It is considered the second most important family of predatory mites in citrus orchards in Brazil. However, their identification, especially that of the members of the genus Agistemus, is rather difficult based only on morphology. Hence, this study describes the use of random amplified polymorphic DNA (RAPD-PCR) markers to determine the genetic similarity of an Agistemus pallinii Matioli et al population found in 2004 in a citrus orchard in Viçosa, Minas Gerais State, Brazil, preying on Panonychus citri (McGregor). Amplifi cations were performed with 12 random primers (OPAA8, OPAA19, OPAB1, OPAB5, OPAB18, OPAC9, OPAC17, OPAC19, OPAD10, OPAE9, OPAE12 and OPAE17),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Predatory mite; Genetic variability; Molecular marker.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200015

Genetic similarity among male bees of Euglossa truncata Rebêlo &amp;amp; Moure (Hymenoptera: Apidae) revealed by RAPD markers
Provedor de dados: 82
Autores: Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Pascual,Amália N.T.; Sofia,Silvia H.
The genetic RAPD (random amplified polymorphic DNA) markers have been used successfully in taxonomical studies of several groups of organisms. In the present study these molecular markers were used to analyze the genetic similarity among eighteen males of Euglossa truncata Rebêlo &amp;amp; Moure exhibiting variations in two morphological characters (colour of the antennal scape and metaepisternal hairs) which are frequently used to identify this species of euglossine bee. The twelve primers used in the RAPD analysis amplified 127 loci, of which 40 (31.5%) were polymorphic, showing some variation among the individuals. The coefficients of genetic similarity among the individuals ranged from 0.79 to 0.95, indicating a rather high genetic similarity among the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000400008

Evaluation of the RAPD profiles from different body parts of Euglossa pleosticta Dressler male bees (Hymenoptera: Apidae, Euglossina)
Provedor de dados: 82
Autores: Pascual,Amália N.T.; Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Sofia,Silvia H.
In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P &lt; 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014

Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis
Provedor de dados: 82
Autores: Sosa-Gomez,Daniel R.; Delpin,Katiaíres E.; Almeida,Alvaro M.R.; Hirose,Edson.
The Neotropical Brown Stink Bug (NBSB), Euschistus heros (Fabricius), has a wide distribution in Brazilian soybean fields, being more important in the central region of the country. The species is the main target of insecticide applications for stink bug pest control. We determined the variability among and within populations of the NBSB by random amplification of polymorphic DNA (RAPD) analysis. Samples of NBSB were collected in soybean fields from Ubiratã (PR), Londrina (PR), Centenário do Sul (PR), Cândido Mota (SP), Ponta Porã (MS) and Sapezal (MT). Genomic DNA was extracted from the head to minimize DNA contamination by endoparasites. DNA was amplified with 10 mer primers. Fifteen primers produced 246 bands. The genetic similarity matrix was obtained,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; Molecular marker; Genetic structure; Gene flow.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000200009

Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)
Provedor de dados: 83
Autores: Joya,Cristhian Danilo; Landínez-García,Ricardo Marcel; Márquez,Edna Judith.
ABSTRACT Pimelodus yuma (formerly Pimelodus blochii) is a freshwater fish, endemic to the Colombian Magdalena-Cauca and Caribbean basins that experiences habitat disturbances resulting from anthropogenic activities. Due to the lack of information about the population genetics of this species, this study developed 14 species-specific microsatellite loci to assess the genetic diversity and population structure of samples from the lower section of the Cauca River. The studied species showed genetic diversity levels higher than the average values reported for Neotropical Siluriformes and significant inbreeding levels as was described for some congeners. Furthermore, P. yuma comprises two coexisting genetic groups that exhibit gene flow along the lower section...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Genetic diversity; Gene flow; Molecular marker; Next generation sequencing.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100209

Population genetics of the endangered catfish Pseudoplatystoma magdaleniatum (Siluriformes: Pimelodidae) based on species-specific microsatellite loci
Provedor de dados: 83
Autores: García-Castro,Kevin León; Rangel-Medrano,José David; Landínez-García,Ricardo Marcel; Márquez,Edna Judith.
ABSTRACT The Neotropical catfish genus Pseudoplatystoma comprises eight species of large size, widely distributed in South American basins. The endangered species P. magdaleniatum is endemic to Magdalena basin (Colombia), experiences high fishing pressure and its population genetics is relatively unknown. To study the genetic status and structure of P. magdaleniatum, 25 species-specific polymorphic microsatellite loci were developed using next-generation sequencing and then tested in samples collected in the Magdalena-Cauca basin. Based on 15 of these loci, P. magdaleniatum showed a high number of alleles per locus (9-10), high values of observed (0.762-0.798) and expected (0.770-0.791) heterozygosities, recent reduction of population size and gene flow....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bottleneck; Freshwater fish; Gene flow; Genetic diversity; Molecular marker.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100215

Increased grain dormancy in white-grained wheat by introgression of preharvest sprouting tolerance QTLs
Provedor de dados: 19
Autores: Kottearachchi, NS; Uchino, N.; Kato, Kiyoaki; Miura, Hideho; 加藤, 清明; 三浦, 秀穂.
White-grained wheat cultivars have long been recognized to be less resistant to preharvest sprouting (PHS) than the red-grained ones. Previously two QTLs for grain dormancy, QPhs.ocs-3A.1 (QPhs-3AS) and QPhs.ocs-4A.1 (QPhs-4AL) were identified in a highly dormant Japanese red wheat, Zenkoujikomugi (Zen). Aiming at improvement of PHS tolerance in white-grained wheat, the introgression effect of these two QTLs in a white-grained population consisting of 40 recombinant inbred lines (RILs) developed from a cross between Zen and white-grained Spica was examined here. Random 20 RILs with red grains were also developed from the same cross and used as a control population. The RILs were grown in the field and in the glasshouse to evaluate the grain dormancy by...
Palavras-chave: Grain dormancy; Molecular marker; QTL; Triticum aestivum; White-grained wheat.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/714

Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers
Provedor de dados: 86
Autores: Mulato,Bruno Mello; Möller,Milene; Zucchi,Maria Imaculada; Quecini,Vera; Pinheiro,José Baldin.
The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Molecular marker; Plant breeding; Plant germplasm; Polymorphism information content.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000300007

Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers
Provedor de dados: 86
Autores: Queiroz,Paulo Roberto; Martins,Erica Soares; Klautau,Nazaré; Lima,Luzia; Praça,Lilian; Monnerat,Rose Gomes.
Abstract: The objective of this work was to develop sequence-characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT-B1 and BT-B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT-BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aleyrodidae; Commodities; Field samples; Molecular marker; Sweet potato whitefly.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500555

Genetic diversity of sweet sorghum germplasm in Mexico using AFLP and SSR markers
Provedor de dados: 86
Autores: Pecina‑Quintero,Víctor; Anaya‑López,José Luis; Zamarripa‑Colmenero,Alfredo; Montes‑García,Noe; Nuñez‑Colín,Carlos; Solis‑Bonilla,Jose Luis; Aguilar‑Rangel,María Rocío; Prom,Louis.
The objective of this work was to evaluate the diversity and genetic relationships between lines and varieties of the sweet sorghum (Sorghum bicolor) germplasm bank of the National Institute for Forestry, Agriculture and Livestock Research, Mexico, using AFLP and SSR markers. The molecular markers revealed robust amplification profiles and were able to differentiate the 41 genotypes of sweet sorghum evaluated. Analysis of the frequency and distribution of polymorphic fragments allowed for the detection of unique (AFLP) and rare (SSR) alleles in several genotypes (RBSS‑8, RBSS‑9, RBSS‑25, RBSS‑32, and RBSS‑37), indicating that these markers may be associated with a feature that has not yet been determined or may be useful for the identification of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sorghum bicolor; Biofuels; Genetic improvement; Molecular marker; Rare allele.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800009

Rhizosphere bacterial communities of potato cultivars evaluated through PCR-DGGE profiles
Provedor de dados: 86
Autores: Ferreira,Enderson Petrônio de Brito; Dusi,André Nepomuceno; Xavier,Gustavo Ribeiro; Rumjanek,Norma Gouvêa.
The objective of this work was to determine the shifts on the PCR-DGGE profiles of bacterial communities associated to the rhizosphere of potato cultivars, in order to generate baseline information for further studies of environmental risk assessment of genetically modified potato plants. A greenhouse experiment was carried out with five potato cultivars (Achat, Bintje, Agata, Monalisa and Asterix), cultivated in pots containing soil from an integrated system for agroecological production. The experiment was conducted in a split plot randomized block design with five cultivars, three sampling periods and five replicates. Rhizosphere samples were collected in three sampling dates during plant development. DNA of rhizosphere microorganisms was extracted,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Associative bacteria; Molecular marker; PCA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500008

Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism
Provedor de dados: 92
Autores: Zaleski,Scheila R. M.; Lazzari,Sonia M. N.; Lazzarotto,Crisleide M.; Panzavolta,Tiziana; Iede,Edson T.; Marques,Francisco de A.
Genetic structure of populations of Pissodes castaneus (De Geer) (Coleoptera, Curculionidae) using amplified fragment length polymorphism. The objective of this study was to determine the genetic structure of populations of Pissodes castaneus from different areas and on different species of Pinus using the PCR-AFLP technique. Twenty samples were analyzed, representing 19 populations from Brazil and one from Florence, Italy, which is the region of origin of P. castaneus. The four combinations of primers generated a total of 367 fragments of DNA, and 100% of polymorphic loci, indicating high degree of molecular polymorphism. The dendrogram did not reveal trends for grouping the populations in relation to origin. The low genetic similarity (0.11 between the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Banded pine weevil; Insecta; Molecular marker; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262013000400010

Cucumber mosaic virus resistance and reproductive biology of brazilian melon accessions
Provedor de dados: 94
Autores: Sanabria-Verón,Nadia Carolina; Melo,Cláusio Antônio Ferreira de; Pereira,Jadergudson; Nunes,Glauber Henrique de Sousa; Oliveira,Ohana Luiza Santos de; Corrêa,Ronan Xavier.
Abstract  In melon (Cucumis melo) the resistance to Cucumber Mosaic Virus (CMV) and the knowledge about reproductive characteristics are important for selection of genitors for breeding population. So, the objective was to investigate if different accessions of melon from a Brazilian germplasm bank have variability in resistance to CMV (FNY strain) and polymorphism in resistance loci markers, as well as whether there is genetic diversity on resistance and floral morphology descriptors and reproductive biology, aiming at genetic improvement. The symptoms induced by CMV were evaluated in 52 melon genotypes which were genotyped with three markers of single nucleotide polymorphisms related to CMV resistance. A sample of 24 genotypes was characterized for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular marker; SNP-CAP; Genetic diversity; Floral and reproductive variability; Ward-MLM; CMV.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000500405

Assessment of genetic diversity in the Russian olive (Elaeagnus angustifolia) based on ISSR genetic markers
Provedor de dados: 124
Autores: Asadiar,Leila Sadat; Rahmani,Fatemeh; Siami,Abbas.
Elaeagnus is a Eurasian tree with 77 species worldwide. In this study, ISSR markers were used to establish the level of genetic relationships and polymorphism across nine genotypes of Elaeagnus angustifolia collected from 9 different regions of West Azarbaijan province. The ISSR analysis with 11 anchored primers also generated 116 scorable loci, of which 92 were polymorphic (79.3%). The estimated Jaccard similarity coefficient ranged from 0.44 to 0.76 for the ISSR markers. Cluster analysis was carried out, based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) and the dendrogram drawn with the help of the NTSYSpc 2.02 software. The analysis revealed 5 main clusters for the ISSR data. According to our results, there is a relatively high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrogram; Russian olive; Genetic variation; Molecular marker.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902013000200013

Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies
Provedor de dados: 63
Autores: Nascimento,Ildon Rodrigues do; Maluf,Wilson Roberto; Figueira,Antônia Reis; Menezes,Cícero Beserra; Resende,Juliano Tadeu Vilela de; Faria,Marcos Ventura; Nogueira,Douglas Willian.
The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lycopersicon esculentum; Sw-5; Vírus resistance; Molecular marker.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300003

Simulation of mixed models in augmented block design
Provedor de dados: 63
Autores: Santos,Aladir Horacio; Bearzoti,Eduardo; Ferreira,Daniel Furtado; Silva Filho,João Luís da.
The augmented block design is widely used in breeding programs, with non-replicated treatments generally being selection units, and replicated treatments being standard cultivars. Originally, an intrablock analysis (fixed model) was proposed. Although non-replicated treatments and/or blocks can be considered of random nature, mixed linear models could be used instead. This work evaluated such an approach, using computer simulation. Populations consisted of sets of randomly generated inbred lines. Molecular marker data were also simulated to allow the estimation of the genetic covariance matrix. Different conditions were considered, varying heritability and the coefficient b of Smith of soil heterogeneity. For each condition 100 simulations were performed,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Augmented design; Mixed model; Molecular marker.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000300012

Molecular fingerprinting of Fusarium oxysporum f. sp. passiflorae isolates using AFLP markers
Provedor de dados: 63
Autores: Silva,Aline dos Santos; Oliveira,Eder Jorge de; Haddad,Fernando; Jesus,Onildo Nunes de; Oliveira,Saulo Alves Santos de; Costa,Maria Angélica Pereira de Carvalho.
Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder &amp;amp; H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passion fruit; Fusarium wilt; Fingerprint; Variability; Molecular marker.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200008

การใช้เครื่องหมายโมเลกุลชนิด STSs สำรวจแหล่งพันธุกรรมยีนต้านทาน
Provedor de dados: 106
Autores: Payorm Cobelli; Jittima Wongnongwa; Varapong Chamarerk; Somsong Chotechuen; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nuchakarn Sinprasit; Teerada Wangsomboondee.
Bacterial blight (BB) disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in all major rice-growing areas of Thailand.  The most effective approach to control BB is the use of resistant varieties. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. Therefore, using genetic resources conferring different resistance genes are more advantage in rice improvement.  The aim of this research was to survey and screen BB resistance genes in Thai local rice varieties from the National Rice Seed Storage Laboratory for Genetic Resources to gain more genetic diversity for resistance.  In this study, the Thai Local rice...
Tipo: PhysicalObject
Palavras-chave: Bacterial blight; Molecular marker; BB resistance genes; BB greenhouse screening; โรคขอบใบแห้ง; เครื่องหมายโมเลกุล; ยีนต้านทานโรคขอบใบแห้ง; การคัดเลือก; สภาพเรือนทดลอง; ความต้านทานต่อโรค.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5652

การใช้ประโยชน์เครื่องหมายโมเลกุลชนิด STS สำหรับงานปรับปรุงพันธุ์ข้าวไทย ให้ต้านทานต่อโรคขอบใบแห้งแบบคงทนถาวร
Provedor de dados: 106
Autores: Payorm Cobelli; Somjai Saleeto; Teerada Wangsomboondee; Anuchart Kotchasatit; Jittima Wongnongwa; Varapong Chamarerk; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nutchakarn Sinprasit; Pornparn Theerattha; Sirada Oncharoen.
Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties which is economically feasible, an environmental-friendly control method and easy for farmers to handle. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistance genes. A feasible method to solve this problem is by pyramiding multiple resistance genes, which are board spectrum, into susceptible rice varieties. The aims of this research were to identify the effectiveness of board spectrum BB resistance genes and to better understand the...
Tipo: Collection
Palavras-chave: Bacterial blight; Resistance gene; Molecular marker; Board spectrum resistance; Durable resistance; โรคขอบใบแห้ง; ยีนต้านทานโรคขอบใบแห้ง; เครื่องหมายโมเลกุลชนิด STS; ต้านทานแบบกว้างขวาง; ต้านทานแบบคงทนถาวร.
Ano: 2015
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5857

การยืนยันการถ่ายทอดลักษณะความหอมและเครื่องหมายดีเอ็นเอที่เชื่อมโยงกับความหอมในถั่วเหลืองฝักสดพันธุ์ Chamame
Provedor de dados: 106
Autores: Thongchai Chanchu; Sompong Chankaew; Ruangchai Juwattanasomran; Peerasak Srenives; Prakit Somta.
Vegetable soybean is an important exported crop for export of Thailand. Fragrance is a trait  determining vegetable soybean quality flavored by customers and can increase the value-added products of vegetable soybean. Fragrance is mainly caused by volatile chemical, 2-Acetyl-1-pyrroline (2AP). The objective of this study was to confirm the mode of inheritance of fragrance in vegetable soybean variety “chamame” and the association between functional marker GmBADH2-10 and fragrance determined by sensory test in the F2 population derived from cross between #75 (non-fragrant) x Chamame (fragrant). The results showed that the fragrance segregated into 3 (non-fragrance): 1 (fragrance) ratio, indicating that fragrance is controlled by a single recessive gene. DNA...
Tipo: Collection
Palavras-chave: Fragrance; Molecular marker; Vegetable soybean; ถั่วเหลืองฝักสด; เครื่องหมายโมเลกุล; ความหอม; แถบดีเอ็นเอ; การปรับปรุงพันธุ์; การถ่ายทอดลักษณะพันธุกรรม.
Ano: 2011
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5177

การปรับปรุงพันธุ์ข้าวเหนียวสายพันธุ์แม่โจ้ 2 จากข้าวเจ้าพันธุ์ปทุมธานี 1 ด้วยวิธีผสมกลับ เเละใช้เครื่องหมายโมเลกุลช่วยในการคัดเลือก
Provedor de dados: 106
Autores: Varaporn Sangtong; Natdanai Subsomboon; Jetsarun Suwanthanee; Suphasuda Inbualuang; Anurak Polyieam; Jirapha Pano; Benjawan Lhakawee.
“Maejo 2” is a new variety of glutinous rice with desirable aroma, lacking photoperiod sensitivity and exhibiting a semi-dwarf stature. Glutinous rice Maejo 2 was improved from the recurrent non-glutinous rice parent, Pathum Thani 1 (WxWx), which has desirable aroma, lacks photoperiod sensitivity and has a semi-dwarf stature. Pathum Thani 1 was crossed with a glutinous rice (wxwx) donor parent, RD6 to initiate a backcross breeding program. Molecular markers for the wx (glutinous, recessive) or Wx (normal, dominant) alleles were used to identify progeny that inherited the desired wx allele in heterozygous individuals that don’t express the glutinous phenotype. Thus, each BCnF1 generation, F1 progeny with the of backcrossing in this way, the BC4F1 plants...
Tipo: Collection
Palavras-chave: Maejo 2; Pathum Thani 1; Non glutinous rice; Glutinous rice; Molecular marker; ข้าวเจ้า; พันธุ์ปทุมธานี 1; พันธุ์แม่โจ้ 2; ข้าวเหนียว; การปรับปรุงพันธุ์; เครื่องหมายโมเลกุล; วิธีผสมกลับ; การคัดเลือกพันธุ์.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5589

การใช้เครื่องหมายโมเลกุลชนิด STSs สำรวจแหล่งพันธุกรรมยีนต้านทาน  ต่อโรคขอบใบแห้งของข้าวพันธุ์พื้นเมืองไทยที่เก็บรวบรวมไว้ที่  ศูนย์ปฏิบัติการและเก็บเมล็ดเชื้อพันธุ์ข้าวแห่งชาติ
Provedor de dados: 106
Autores: Payorm Cobelli; Jittima Wongnongwa; Varapong Chamarerk; Somsong Chotechuen; Rasamee Dhitikiattipong; Witchuda Rattanakarn; Nuchakarn Sinprasit; Teerada Wangsomboondee.
Bacterial blight (BB) disease of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases in all major rice-growing areas of Thailand. The most effective approach to control BB is the use of resistant varieties. However, the selection pressure from the usage of resistant cultivars may result in mutation of the pathogen population to overcome the resistant genes. Therefore, using genetic resources conferring different resistant genes are more advantage in rice improvement. The aim of this research was to survey and screen for BB resistant genes in Thai local rice varieties from the National Rice Seed Storage Laboratory for Genetic Resources to gain more genetic diversity for the resistance. In this study, the Thai Local rice...
Tipo: Collection
Palavras-chave: Bacterial Blight (BB); Molecular marker; BB resistance genes; BB greenhouse screening; โรคขอบใบแห้ง; เครื่องหมายโมเลกุล; ยีนต้านทานโรคขอบใบแห้ง; การคัดเลือกความต้านทานต่อโรคขอบใบแห้งในสภาพเรือนทดลอง.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5935