Consulta: subjectFacets:"MtDNA"
Registros recuperados: 69
Data/hora: 06/06/2024 05:32:14

Variabilidad genética y relación filogeográfica de tres subespecies de venado cola blanca (Odocoileus virginianus) en la región Centro-Norte de México.
Provedor de dados: 32
Autores: Hernández Llamas, Alán Roberto.
El venado cola blanca es la especie de mayor importancia en la actividad cinegética en México. En el país se distribuyen 14 de las 38 subespecies reportadas para Norte, Centro y Sur América, a pesar de esto existe poco conocimiento acerca de su diversidad genética. El objetivo de esta investigación fue determinar y describir la variabilidad genética de las subespecies O. v. miquihuanensis, O. v. couesi y O. v. mexicanus en la región Centro-Norte de México por medio del uso de un fragmento de la Región Control (D-Loop) del ADN mitocondrial. Se colectaron 67 muestras de tejido muscular, cartílago auricular u órganos; 4 de O. v. miquihuanensis, 43 O. v. mexicanus, y 20 O. v. couesi de Zacatecas, Aguascalientes, San Luis Potosí, Morelos y Puebla. Se...
Palavras-chave: Odocoileus virginianus; ADNmt; Región Control; Diversidad Genética; MtDNA; D-Loop; Genetic Diversity; Ganadería; Maestría.
Ano: 2014
URL: http://hdl.handle.net/10521/2246

High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region
Provedor de dados: 74
Autores: Mazumder,Sabuj Kanti; Alam,Md. Samsul.
The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic variability; PCR-RFLP; MtDNA; Tenualosa ilisha.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100029

Maternal transmission of mitochondrial diseases
Provedor de dados: 74
Autores: Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308

Genetic structure of honeybee populations from southern Brazil and Uruguay
Provedor de dados: 74
Autores: Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008

Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin
Provedor de dados: 74
Autores: Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006

Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation
Provedor de dados: 74
Autores: Tagliaro,Claudia Helena; Schneider,Horacio; Sampaio,Iracilda; Schneider,Maria Paula Cruz; Vallinoto,Marcelo; Stanhope,Michael.
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Saguinus; Callitrichinae; ND1; Phylogeny; MtDNA; Conservation.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100009

Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus)
Provedor de dados: 74
Autores: Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005

Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic
Provedor de dados: 74
Autores: Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p &lt; 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p &lt; 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026

Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A. m. lamarckii
Provedor de dados: 74
Autores: Kandemir,Irfan; Pinto,Maria A.; Meixner,Marina D.; Sheppard,Walter S.
Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Cytochrome oxidase I; Apis mellifera lamarckii; O lineage.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400027

Molecular genetic diversity in populations of the stingless bee Plebeia remota: A case study
Provedor de dados: 74
Autores: Francisco,Flávio de Oliveira; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Genetic diversity is a major component of the biological diversity of an ecosystem. The survival of a population may be seriously threatened if its genetic diversity values are low. In this work, we measured the genetic diversity of the stingless bee Plebeia remota based on molecular data obtained by analyzing 15 microsatellite loci and sequencing two mitochondrial genes. Population structure and genetic diversity differed depending on the molecular marker analyzed: microsatellites showed low population structure and moderate to high genetic diversity, while mitochondrial DNA (mtDNA) showed high population structure and low diversity in three populations. Queen philopatry and male dispersal behavior are discussed as the main reasons for these findings.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Meliponini; Microsatellites; Philopatry; Population genetics.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100017

Phylogeography and RAPD-PCR variation in Hoplias malabaricus (Bloch, 1794) (Pisces, Teleostei) in southeastern Brazil
Provedor de dados: 74
Autores: Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio.
In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Nei’s genetic distance) suggest the existence of two genetically different groups, one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005

Human aging and somatic point mutations in mtDNA: a comparative study of generational differences (grandparents and grandchildren)
Provedor de dados: 74
Autores: Marinho,Anderson Nonato do Rosário; Moraes,Milene Raiol de; Santos,Sidney; Santos,Ândrea Ribeiro-dos-.
The accumulation of somatic mutations in mtDNA is correlated with aging. In this work, we sought to identify somatic mutations in the HVS-1 region (D-loop) of mtDNA that might be associated with aging. For this, we compared 31 grandmothers (mean age: 63 ± 2.3 years) and their 62 grandchildren (mean age: 15 ± 4.1 years), the offspring of their daughters. Direct DNA sequencing showed that mutations absent in the grandchildren were detected in a presumably homoplasmic state in three grandmothers and in a heteroplasmic state in an additional 13 grandmothers; no mutations were detected in the remaining 15 grandmothers. However, cloning followed by DNA sequencing in 12 grandmothers confirmed homoplasia in only one of the three mutations previously considered to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aging; MtDNA; Mitochondrial somatic mutations.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100006

Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens
Provedor de dados: 74
Autores: Santos,Sonia Maria Lima; Tagliaro,Claudia Helena; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Santos Filho,Carlos; Müller,Ana Cláudia de Paula.
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Teredinidae; RRNA16S; Phylogeny; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100031

Genetic signatures of parental contribution in black and white populations   in Brazil
Provedor de dados: 74
Autores: Guerreiro-Junior,Vanderlei; Bisso-Machado,Rafael; Marrero,Andrea; Hünemeier,Tábita; Salzano,Francisco M.; Bortolini,Maria Cátira.
Two hundred and three individuals classified as white were tested for 11 single nucleotide polymorphisms plus two insertion/deletions in their Y-chromosomes. A subset of these individuals (n = 172) was also screened for sequences in the first hypervariable segment of their mitochondrial DNA (mtDNA). In addition, complementary studies were done for 11 of the 13 markers indicated above in 54 of 107 black subjects previously investigated in this southern Brazilian population. The prevalence of Y-chromosome haplogroups among whites was similar to that found in the Azores (Portugal) or Spain, but not to that of other European countries. About half of the European or African mtDNA haplogroups of these individuals were related to their places of origin, but not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Admixture dynamics; MtDNA; Y-chromosome markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100001

Tocantins river as an effective barrier to gene flow in Saguinus niger populations
Provedor de dados: 74
Autores: Vallinoto,Marcelo; Araripe,Juliana; Rego,Péricles S. do; Tagliaro,Claudia H.; Sampaio,Iracilda; Schneider,Horacio.
The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Primates; Saguinus niger; D-loop; MtDNA; Tocantins river.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200005

Uniparental ancestry markers in Chilean populations
Provedor de dados: 74
Autores: Vieira-Machado,Camilla Dutra; Tostes,Maluah; Alves,Gabrielle; Nazer,Julio; Martinez,Liliana; Wettig,Elisabeth; Pizarro Rivadeneira,Oscar; Diaz Caamaño,Marcela; Larenas Ascui,Jessica; Pavez,Pedro; Dutra,Maria da Graça; Castilla,Eduardo Enrique; Orioli,Ieda Maria.
Abstract The presence of Native Americans, Europeans, and Africans has led to the development of a multi-ethnic, admixed population in Chile. This study aimed to contribute to the characterization of the uniparental genetic structure of three Chilean regions. Newborns from seven hospitals in Independencia, Providencia, Santiago, Curicó, Cauquenes, Valdívia, and Puerto Montt communes, belonging to the Chilean regions of Santiago, Maule, and Los Lagos, were studied. The presence of Native American mitochondrial DNA (mtDNA) haplogroups and two markers present in the non-recombinant region of the Y chromosome, DYS199 and DYS287, indicative of Native American and African ancestry, respectively, was determined. A high Native American matrilineal contribution and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Uniparental markers; Ancestry; MtDNA; Y-chromosome; ECLAMC.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400573

A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes)
Provedor de dados: 74
Autores: Toffoli,Daniel; Hrbek,Tomas; Araújo,Maria Lúcia Góes de; Almeida,Maurício Pinto de; Charvet-Almeida,Patricia; Farias,Izeni Pires.
DNA barcoding is a recently proposed global standard in taxonomy based on DNA sequences. The two main goals of DNA barcoding methodology are assignment of specimens to a species and discovery of new species. There are two main underlying assumptions: i) reciprocal monophyly of species, and ii) intraspecific divergence is always less than interspecific divergence. Here we present a phylogenetic analysis of the family Potamotrygonidae based on mitochondrial cytochrome c oxidase I gene, sampling 10 out of the 18 to 20 valid species including two non-described species. Potamotrygonidae systematics is still not fully resolved with several still-to-be-described species while some other species are difficult to delimit due to overlap in morphological characters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Potamotrygon; Barcoding; Radiation; Phylogenetics; COI; MtDNA.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200028

Green turtle (Chelonia mydas) genetic diversity at Paranaguá Estuarine Complex feeding grounds in Brazil
Provedor de dados: 74
Autores: Jordão,Juliana Costa; Bondioli,Ana Cristina Vigliar; Guebert,Flavia Maria; Thoisy,Benoit de; Toledo,Lurdes Foresti de Almeida.
Sea turtles are marine reptiles that undertake long migrations through their life, with limited information regarding juvenile stages. Feeding grounds (FGs), where they spend most of their lives, are composed by individuals from different natal origins, known as mixed stock populations. The aim of this study was to assess genetic composition, natal origins and demographic history of juvenile green turtles (Chelonia mydas) at the Paranaguá Estuarine Complex (PEC), Brazil, considered a Natural World Heritage site. Tissue samples of stranded animals were collected (n = 60), and 700 bp mitochondrial DNA sequences were generated and compared to shorter sequences from previously published studies. Global exact tests of differentiation revealed significant...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Chelonia mydas; MtDNA; Feeding grounds; Mixed stock analysis; Connectivity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300346

Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion
Provedor de dados: 74
Autores: Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena.
Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033

Site heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus
Provedor de dados: 74
Autores: Dudu,Andreea; Georgescu,Sergiu Emil; Berrebi,Patrick; Costache,Marieta.
Sturgeons are fish species with a complex biology. They are also characterized by complex aspects including polyploidization and easiness of hybridization. As with most of the Ponto-Caspian sturgeons, the populations of Acipenser ruthenus from the Danube have declined drastically during the last decades. This is the first report on mitochondrial point heteroplasmy in the cytochrome b gene of this species. The 1141 bp sequence of the cytb gene in wild sterlet sturgeon individuals from the Lower Danube was determined, and site heteroplasmy evidenced in three of the 30 specimens collected. Two nucleotide sequences were identified in these heteroplasmic individuals. The majority of the heteroplasmic sites are synonymous and do not modify the sequence of amino...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sterlet sturgeon; MtDNA; Site heteroplasmy.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500024

Genetic characterization of native and introduced populations of the neotropical cichlid genus Cichla in Brazil
Provedor de dados: 74
Autores: Carvalho,Daniel Cardoso de; Oliveira,Denise Aparecida Andrade de; Santos,José Enemir dos; Teske,Peter; Beheregaray,Luciano B.; Schneider,Horacio; Sampaio,Iracilda.
A molecular phylogenetic analysis based on mitochondrial 16S ribosomal DNA and Control Region sequences from native and introduced populations was undertaken, in order to characterize the introduction of Cichla (peacock bass or tucunaré) species in Brazil. Mitochondrial DNA haplotypes found in introduced fish from Minas Gerais state (southeastern Brazil) clustered only with those from native species of the Tocantins River (Cichla piquiti and C. kelberi), thereby suggesting a single or, at most, few translocation acts in this area, even though with fish from the same source-population. Our study contributes to an understanding of the introduction of Cichla in regions of Brazil outside the Amazon basin, and adds phylogenetic data to the recently describe...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peacock bass or tucunaré; MtDNA; Invasive populations; Amazon basin; Minas Gerais.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300025

Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil
Provedor de dados: 74
Autores: Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031

Genetic variation and evolutionary demography of Fenneropenaeus chinensis populations, as revealed by the analysis of mitochondrial control region sequences
Provedor de dados: 74
Autores: Kong,Xiao Yu; Li,Yu Long; Shi,Wei; Kong,Jie.
Genetic variation and evolutionary demography of the shrimp Fenneropenaeus chinensis were investigated using sequence data of the complete mitochondrial control region (CR). Fragments of 993 bp of the CR were sequenced for 93 individuals from five localities over most of the species' range in the Yellow Sea and the Bohai Sea. There were 84 variable sites defining 68 haplotypes. Haplotype diversity levels were very high (0.95 ± 0.03-0.99 ± 0.02) in F. chinensis populations, whereas those of nucleotide diversity were moderate to low (0.66 ± 0.36%-0.84 ± 0.46%). Analysis of molecular variance and conventional population statistics (F ST) revealed no significant genetic structure throughout the range of F. chinensis. Mismatch distribution, estimates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fenneropenaeus chinensis; MtDNA; Isolation with migration (IM) coalescence; Historical demography; Population expansion.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200029

Genetic distance between broodstocks of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses
Provedor de dados: 74
Autores: Francisco,Ana Karina de; Galetti Junior,Pedro Manoel.
In the constantly growing Brazilian shrimp industry the evaluation of genetic relationships between broodstocks is an useful tool for shrimp culture management programs. We established the genetic relationships between five broodstocks of the white marine shrimp Litopenaeus vannamei (Penaeidae) based on the sequencing of the mtDNA 16S rRNA and cytochrome oxidase I (COI) regions. Although no divergence was found between the broodstocks for the highly conservative 16S rRNA gene, we did find an 8.2% distance between L. vannamei and the Farfantepenaeus subtilis. Analyses of the COI region showed genetic distances of only 0.2 to 1% between the broodstocks, which contrasted with the 10.9% mean distance found between L. vannamei and F. subtilis. The small genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Shrimp; Litopenaeus vannamei; Genetic variation; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200014

Restriction fragment length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae)
Provedor de dados: 74
Autores: Moysés,Cinthia Bachir; Almeida-Toledo,Lurdes F. de.
Restriction fragment length polymorphism (RFLP) analysis of mitochondrial DNA (mtDNA) was employed to characterize species and populations of Astyanax, a Neotropical freshwater fish genus. Samples of five species, A. altiparanae, A. fasciatus, A. lacustris, A. scabripinnis paranae and A. schubarti, from the Upper Paraná and São Francisco river basins were analyzed. Two out of the ten restriction enzymes employed generated species-specific mtDNA patterns for each of the five species. MtDNA exhibited considerable polymorphism within and among populations. All populations sampled showed relatively high values of haplotype diversity. Geographically localized haplotypes were detected for A. altiparanae and A. fasciatus from the Upper Paraná and São Francisco...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; RFLP; Astyanax.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400008

Genetic structure in two northern muriqui populations (Brachyteles hypoxanthus, Primates, Atelidae) as inferred from fecal DNA
Provedor de dados: 74
Autores: Fagundes,Valéria; Paes,Marcela F.; Chaves,Paulo B.; Mendes,Sérgio L.; Possamai,Carla de B.; Boubli,Jean P.; Strier,Karen B.
We assessed the genetic diversity of two northern muriqui (Brachyteles hypoxanthus Primata, Atelidae) populations, the Feliciano Miguel Abdala population (FMA, n = 108) in the Brazilian state of Minas Gerais (19°44' S, 41°49' W) and the Santa Maria de Jetibá population (SMJ, n = 18) in the Brazilian state of Espírito Santo (20°01' S, 40°44' W). Fecal DNA was isolated and PCR-RFLP analysis used to analyze 2160 bp of mitochondrial DNA, made up of an 820 bp segment of the gene cytochrome c oxidase subunit 2 (cox2, EC 1.9.3.1), an 880 bp segment of the gene cytochrome b (cytb, EC 1.10.2.2) and 460 bp of the hypervariable segment of the mtDNA control region (HVRI). The cox2 and cytb sequences were monomorphic within and between populations whereas the HVRI...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Brachyteles; Conservation genetics; Fecal DNA; MtDNA; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100028

Genetic characterisation of populations of the critically endangered Goliath grouper ( Epinephelus itajara,  Serranidae) from the Northern Brazilian coast through analyses of mtDNA
Provedor de dados: 74
Autores: Silva-Oliveira,Gláucia C.; Rêgo,Péricles S. do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The Goliath grouper ( Epinephelus itajara ) is one of the most endangered species of fish of the subfamily Epinephelinae. Slow to develop and mature, and dependent on mangrove habitats for breeding, the species also suffers intense harvesting, which has reduced drastically in numbers in many areas. To contribute to the understanding of the characteristics of  E. itajara  populations, we conducted a molecular genetics study of the species, focusing on populations from the Northern Brazilian coast. The mtDNA control region (D-loop) of 116 individuals from five localities (Bragança, Ajuruteua, Parnaíba, Fortaleza and Natal) was analysed, and a sequence of 499 base pairs identified. Analyses of the sequences indicated that genetic variability was generally...
Palavras-chave: Goliath grouper; Epinephelus itajara; Control region; MtDNA; Population genetics.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500029

Phylogenetic relationships in the Drosophila fasciola species subgroup (Diptera, Drosophilidae) inferred from partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene
Provedor de dados: 74
Autores: Silva-Bernardi,Erica C.C.; Morales,Adriana C.; Sene,Fabio M.; Manfrin,Maura H.
The repleta group is one of the largest and most complex species groups in the genus Drosophila, representing an important biological model for the elaboration of evolutionary and biogeographical hypotheses on the American Continent. It is divided into six subgroups, of which the fasciola subgroup is the only one with most of its species found in the humid forests of Central and South America. With the aim of understanding the origin and evolution of the fasciola subgroup, and consequently adding information about the repleta group, we analyzed partial sequences of the mitochondrial cytochrome oxidase subunit I (COI) gene of nine Drosophila species (D. carolinae, D. coroica, D. ellisoni, D. fascioloides, D. ivai, D. moju, D. onca, D. rosinae and D. senei)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fasciola subgroup; Molecular phylogeny; MtDNA; Systematics; Repleta group.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300029

Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns
Provedor de dados: 74
Autores: Souza,Rogério O.; Moretto,Geraldo; Arias,Maria C.; Del Lama,Marco A.
Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Subspecies differentiation; Melipona quadrifasciata; Cytochrome b; MtDNA; PCR-RFLP patterns.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300009

Evidence of geographical structuring in the Malaysian Snakehead, Channa striata based on partial segment of the CO1 gene
Provedor de dados: 74
Autores: Siti-Balkhis,Abu Bakar; Jamsari,Amirul Firdaus Jamaluddin; Hwai,Tan Shau; Yasin,Zulfigar; Siti-Azizah,Mohd Nor.
Channa striata, locally known as "haruan", is economically important in fisheries and aquaculture industries in several Asian countries. DNA sequencing, based on a partial segment of the Cytochrome oxidase c subunit 1 (CO1) gene, was used to determine genetic variation in C. striata samples from four different populations on the west coast of Peninsular Malaysia. The highest nucleotide and haplotype diversities were observed in the Linggi population (π = 0.0067, h = 0.835), and the lowest in the Timah Tasoh population (π = 0.0008, h = 0.286). Apart from Kajang-Linggi, which was insignificant, F ST values were significant (p &lt; 0.05) in all pairwise-population comparisons. Consequently, it is inferred that genetic structuring C. striata populations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Channa striata; Snakehead; MtDNA; CO1; Isolation by distance.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300024

Molecular discrimination of pouched four-eyed opossums from the Mamirauá Reserve in the Brazilian Amazon
Provedor de dados: 74
Autores: Nunes,Cláudia; Ayres,Jose Marcio; Sampaio,Iracilda; Schneider,Horacio.
Previous cytochrome B (CytB) mtDNA studies have suggested four species for the opossum genus Philander (four-eyed opossums), three (P. mcilhennyi, P. andersoni and P. opossum) from the Amazon and one (P. frenata) from the Brazilian Atlantic forest. During a faunal survey nine specimens of Philander sp. and four of Didelphis marsupialis were collected in the Mamirauá Sustainable Reserve, Amazonas State, Brazil. Preliminary analyses based on morphology and geographical distributions were not conclusive, suggesting that Philander specimens could belong to either P. andersoni or P. opossum. In order to elucidate the relationship of this taxon to the remaining Amazonian taxa, seven Philander and two Didelphis specimens animals were sequenced for the cytB mtDNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytochrome B; Mamirauá; Molecular phylogeny; MtDNA; Philander; Pouched four-eyed opossum.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200015

Very low mitochondrial variability in a stingless bee endemic to cerrado
Provedor de dados: 74
Autores: Brito,Rute Magalhães; Francisco,Flávio de Oliveira; Françoso,Elaine; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Partamona mulata is a stingless bee species endemic to cerrado, a severely threatened phytogeographical domain. Clearing for pasture without proper soil treatment in the cerrado facilitates the proliferation of termite ground nests, which are the nesting sites for P. mulata. The genetic consequences of these changes in the cerrado environment for bee populations are still understudied. In this work, we analyzed the genetic diversity of 48 colonies of P. mulata collected throughout the species' distribution range by sequencing two mitochondrial genes, cytochrome oxidase I and cytochrome B. A very low polymorphism rate was observed when compared to another Partamona species from the Atlantic forest. Exclusive haplotypes were observed in two of the five areas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; MtDNA; Genetic variability; Partamona mulata.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100018

Mitochondrial tRNA gene translocations in highly eusocial bees
Provedor de dados: 74
Autores: Silvestre,Daniela; Arias,Maria Cristina.
Mitochondrial gene rearrangement events, especially involving tRNA genes, have been described more frequently as more complete mitochondrial genome sequences are becoming available. In the present work, we analyzed mitochondrial tRNA gene rearrangements between two bee species belonging to the tribes Apini and Meliponini within the "corbiculate Apidae". Eleven tRNA genes are in different genome positions or strands. The molecular events responsible for each translocation are explained. Considering the high number of rearrangements observed, the data presented here contradict the general rule of high gene order conservation among closely related organisms, and also represent a powerful molecular tool to help solve questions about phylogeny and evolution in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Melipona bicolor; Mitogenomics; Gene order; Eusociality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300030

Genetic population structure of the Swordfish (Xiphias gladius) in the southwest Indian Ocean: Sex-biased differentiation, congruency between markers and its incidence in a way of stock assessment
Provedor de dados: 5
Autores: Muths, Delphine; Grewe, R.; Jean, Claire; Bourjea, Jerome.
Genetic variation was surveyed at 11 microsatellite loci and at 517 bp of the mitochondrial control region to investigate the presence of genetic stock structure in swordfish (Xiphias gladius) in four proximal localities of the southwest Indian Ocean. One aim of this study was to serve as a preliminary examination for congruency of structure detected by these two genetic markers, prior to conducting a more comprehensive basin-wide survey of the Indian Ocean and nearby surrounding areas. Analyses of multilocus microsatellite genotypes and mitochondrial control region sequences both revealed a great homogeneity between samples. Genetic diversity detected at the regional scale was not significantly higher than detected at the local scale. Results suggest that...
Tipo: Text
Palavras-chave: Indian Ocean; Population genetic; MtDNA; Microsatellites; Swordfish.
Ano: 2009
URL: http://archimer.ifremer.fr/doc/2009/publication-6455.pdf

Seascape Genetics and the Spatial Ecology of Juvenile Green Turtles
Provedor de dados: 5
Autores: Jensen, Michael P.; Dalleau, Mayeul; Gaspar, Philippe; Lalire, Maxime; Jean, Claire; Ciccione, Stéphane; Mortimer, Jeanne A.; Quillard, Mireille; Taquet, Coralie; Wamukota, Andrew; Leroux, Géraud; Bourjea, Jerome.
Understanding how ocean currents impact the distribution and connectivity of marine species, provides vital information for the effective conservation management of migratory marine animals. Here, we used a combination of molecular genetics and ocean drift simulations to investigate the spatial ecology of juvenile green turtle (Chelonia mydas) developmental habitats, and assess the role of ocean currents in driving the dispersal of green turtle hatchlings. We analyzed mitochondrial (mt)DNA sequenced from 358 juvenile green turtles, and from eight developmental areas located throughout the Southwest Indian Ocean (SWIO). A mixed stock analysis (MSA) was applied to estimate the level of connectivity between developmental sites and published genetic data from...
Tipo: Text
Palavras-chave: Chelonia mydas; Green turtle; Juvenile; MtDNA; Drifting simulation; Connectivity; Mixed stock analysis; Southwest Indian Ocean.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00612/72443/71398.pdf

Population structure enhances perspectives on regional management of the western Indian Ocean green turtle
Provedor de dados: 5
Autores: Bourjea, Jerome; Mortimer, Jeanne A.; Garnier, Julie; Okemwa, Gladys; Godley, Brendan J.; Hughes, George; Dalleau, Mayeul; Jean, Claire; Ciccione, Stephane; Muths, Delphine.
To refine our understanding of the spatial structure of the green turtle (Chelonia mydas) populations in the South West Indian Ocean (SWIO), we analysed patterns of mitochondrial DNA (396 base pairs control region fragment) variation among 171 samples collected at five distinct locations (Kenya, Northern Mozambique, and three locations in the Republic of Seychelles: the Granitic, Amirantes, and Farquhar groups) and compared them to genetic data (n = 288), previously collected from 10 southern locations in the SWIO. We also analysed post-nesting satellite tracks (n = 4) from green turtles nesting in the Amirantes group. Pairwise comparisons of haplotype frequencies showed significant genetic differentiation amongst rookeries and suggest that the SWIO hosts...
Tipo: Text
Palavras-chave: Indian Ocean; MtDNA; Satellite tracking; Phylogeography; Management unit; Chelonia mydas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00266/37732/36062.pdf

Invasion genetics of the Pacific oyster Crassostrea gigas in the British Isles inferred from microsatellite and mitochondrial markers
Provedor de dados: 5
Autores: Lallias, Delphine; Boudry, Pierre; Batista, Frederico; Beaumont, Andy; King, Jonathan W.; Turner, John R.; Lapegue, Sylvie.
The Pacific oyster, Crassostrea gigas, native to northeast Asia, is one of the most important cultured shellfish species. In Europe, Pacific oysters first settled along the Atlantic coasts of France at the end of the 1960s but rapidly spread and are now widely established. Twenty-two sites in the United Kingdom (UK), Ireland, Denmark, France and Spain were sampled to assess genetic diversity and differentiation. Hatchery-propagated stocks from two hatcheries located in the UK also were included. Two main genetic clusters were identified from pairwise genetic differentiation indexes, Bayesian clustering methods or neighbour-joining analysis, based on 7 microsatellite loci: (1) a Northeast cluster (including feral samples from East England, Ireland and...
Tipo: Text
Palavras-chave: Invasion genetics; Marine mollusc; Microsatellites; MtDNA; Pacific oyster; Crassostrea gigas.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00268/37973/36052.pdf

Genetic connectivity of the coral-eating sea star Acanthaster planci during the severe outbreak of 2006-2009 in the Society Islands, French Polynesia
Provedor de dados: 5
Autores: Yasuda, Nina; Taquet, Coralie; Nagai, Satoshi; Yoshida, Terutoyo; Adjeroud, Mehdi.
Occasional population outbreaks of the crown-of-thorns sea star, Acanthaster planci, are a major threat to coral reefs across the Indo-Pacific. The presumed association between the serial nature of these outbreaks and the long larval dispersal phase makes it important to estimate larval dispersal; many studies have examined the population genetic structure of A.planci for this purpose using different genetic markers. However, only a few have focused on reef-scale as well as archipelago-scale genetic structure and none has used a combination of different genetic markers with different effective population sizes. In our study, we used both mtDNA and microsatellite loci to examine A.planci population genetic structure at multiple spatial scales (from &lt;2km...
Tipo: Text
Palavras-chave: Acanthaster planci; Coral reefs; Microsatellite loci; MtDNA; Population genetic structure.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00667/77936/80151.pdf

Sharp genetic break between Atlantic and English Channel populations of the polychaete Pectinaria koreni, along the North coast of France
Provedor de dados: 5
Autores: Jolly, Mt; Jollivet, D; Gentil, F; Thiebaut, E; Viard, F.
This study uses enzymatic and mitochondrial genes to infer the relative importance of historical processes and contemporary hydrodynamic features on the observed patterns of genetic structure in subdivided populations of Pectinaria koreni (Polychaeta: Pectinariidae) along the coasts of Brittany and the English Channel. Nucleotide sequence variation of a 603-bp fragment of the mtDNA cytochrome oxidase subunit I gene revealed a surprisingly deep phylogeographic break of about 16% divergence separating the Brittany and Channel populations, which coincides with a biogeographic boundary along the western coast of Brittany. Deep sequence divergence with fixed haplotype differences and the inversion of allele frequencies at two enzyme loci suggests the occurrence...
Tipo: Text
Palavras-chave: Phylogeography; Allozymes; MtDNA; Parapatric boundary; Cryptic species.
Ano: 2005
URL: https://archimer.ifremer.fr/doc/00301/41250/40433.pdf

Maintenance of genetic differentiation across a transition zone in the sea: discordance between nuclear and cytoplasmic markers
Provedor de dados: 5
Autores: Lemaire, Christophe; Versini, J; Bonhomme, Francois.
To investigate the origin and maintenance of the genetic discontinuity between Atlantic and Mediterranean populations of the common sea bass (Dicentrarchus labrax) we analysed the genetic variation at a fragment of mitochondrial cytochrome b sequence for 18 population samples. The result were also compared with new or previously published microsatellite data. Seven mitochondrial haplotypes and an average nucleotidic divergence of 0.02 between Atlantic and Mediterranean populations that matches a Pleistocene allopatric isolation were found. The frequency variation at the cytochrome b locus was many times greater between Atlantic and Mediterranean populations ((&amp;#952;) over cap (C) = 0.67) than at microsatellite loci ((&amp;#952;) over cap (N) = 0.02)....
Tipo: Text
Palavras-chave: Reproductive isolation; Phylogeography; MtDNA; Microsatellites; Genetic transitions.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-776.pdf

Stock structure of the swordfish (Xiphias gladius) in the southwest Indian Ocean: A preliminary study
Provedor de dados: 5
Autores: Jean, Claire; Bourjea, Jerome; Jouen, Emmanuel; Taquet, Marc.
Stock structure of swordfish (Xiphias gladius Linnaeus, 1758) from four areas of the South West Indian Ocean was conducted using Restriction Fragment Length Polymorphism (RFLP) on mtDNA control region and six amplified microsatellite loci. No significant differences were found among the four locations using either molecular technique. Most variability was found within populations, with only a small proportion of variation among samples, suggesting a unique stock of swordfish in the South West Indian Ocean. The sample size of this study is too small to reveal significant structure among the sampling locations. These results are baseline for a future larger scale project in the whole Indian Ocean.
Tipo: Text
Palavras-chave: Indian Ocean; Microsatellite loci; MtDNA; Xiphias gladius; Swordfish.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2313.pdf

Restricted dispersal of the reef fish Myripristis berndti at the scale of the SW Indian Ocean
Provedor de dados: 5
Autores: Muths, Delphine; Tessier, Emmanuel; Gouws, G.; Craig, M.; Mwale, M.; Mwaluma, J.; Mwandya, A.; Bourjea, Jerome.
The reef fish Myripristis berndti (Jordan &amp; Everman 1903) is a pantropical species. A genetic analysis was conducted on 353 individuals from 10 localities distributed across the SW Indian Ocean (SWIO) in order to determine patterns of connectivity in the SWIO. Both the mtDNA sequences (711-bp cytochrome b sequences) and the microsatellites (8 newly developed loci) reveal spatial patterns of differentiation within the SWIO. There is, however, a discrepancy between the structure observed with each kind of marker. MtDNA revealed that 3 peripheral populations (NW Kenya, SE Reunion, and SW Europa) were isolated from the 7 more central populations, which form a more densely connected population network, while microsatellite data indicated a more restricted...
Tipo: Text
Palavras-chave: MtDNA; Microsatellite; Marine fish; SW Indian Ocean; Connectivity.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00073/18444/15974.pdf

D-loop mitochondrial genetic analysis in Abeerden angus old type from Argentina
Provedor de dados: 46
Autores: Villegas Castagnasso,E.E; Rogberg-Muñoz,A; Prando  ,A.J; Baldo,A; Giovambattista,G.
The massive use of reproductive breeding technologies (mainly Artificial Insemination and Embryo Transfer) resulted in an important decrease in the diversity in many breeds, and some genetic lines have been lost. Within the Angus breed, the &amp;#8220;New Type&amp;#8221; gain importance since 1970, leaving the &amp;#8220;Old Type&amp;#8221; to a reduced number of herds in the whole world. The objective of this work was to determine the genetic diversity in an &amp;#8220;Old Type&amp;#8221; herd in Argentina. DNA samples were analyzed for sequence variation in the hypervariable region of the mitochondrial DNA (D-loop). Sequence comparison and phylogenetic analyses revealed that haplotypes fell into European haplogroup (T3) in general, and in particular had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Bos Taurus; Angus; Matrilineages origin; Genetic diversity.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300003

Molecular and phylogenetic analysis of mitochondrial control region in Robertsonian karyomorphs of Graomys griseoflavus (Rodentia, Sigmodontinae)
Provedor de dados: 81
Autores: Catanesi,Cecilia I.; Vidal-Rioja,Lidia; Zambelli,Andrés.
The South American rodent Graomys griseoflavus is a species with a Robertsonian (Rb) autosomal polymorphism. A marked genetic differentiation between 2n=42-41 and 2n=38-34 karyomorphic groups was evidenced by cytogenetic and molecular analysis. The mitochondrial control region was sequenced in all Graomys karyomorphs for its characterization and used to trace more accurate phylogenetic relationships. The molecular organization showed to be coincident with the consensus molecular structure described for other rodent taxa, exhibiting the conserved domains ETAS (extended termination-associated sequences), CD (central domain) and CSB (conserved sequence block) 1, 2 and 3. Phylogenetic trees showed that 2n=42-41 and 2n=38-34 karyomorphic groups form separate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rodentia; Graomys; Robertsonian polymorphism; MtDNA; Phylogeny.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-93832006000100003

Increased genetic diversity in Greek populations of the genus Ligidium (Crustacea: Isopoda: Oniscidea) revealed by RFLP analysis of mtDNA segments
Provedor de dados: 16
Autores: Klossa-Kilia, E.; Kilias, G.; Sfenthourakis, S.
We investigated mtDNA genetic differentiation and the phylogenetic relationships of 11 populations of the oniscidean genus Ligidium. We studied nine populations from Greece, assigned to three nominal species (L. euboicum, L. germanicum and L. beieri), and two from central Europe (L. germanicum and L. hypnorum), applying RFLP analysis of two mtDNA segments (12S rDNA and 16S rDNA) amplified by PCR. The analysis revealed seven monomorphic and four polymorphic populations, and 17 different haplotypes, each one present in one population only. Several restriction enzymes produced population specific patterns, enabling discrimination among the different populations. The estimated net nucleotide divergence between populations ranges from 1.06 to 16.52%, while the...
Tipo: Article / Letter to the editor
Palavras-chave: Oniscidea; Greece; MtDNA; RLFPs; Genetic isolation; Ligidium; Isopods; 16S rDNA; 12S rDNA; 42.74.
Ano: 2005
URL: http://www.repository.naturalis.nl/record/209650

The phylogenetic position of Artematopodidae (Coleoptera: Elateroidea), with description of the first two Eurypogon species from China
Provedor de dados: 16
Autores: Kundrata, R.; Bocakova, M.; Bocak, L.
The Artematopodidae is a species-poor beetle family with contentious relationships to byrrhoid and elateroid families. Recent molecular phylogenetic analyses brought ambiguous results based on a single sequenced species. We investigated the taxonomic placement of Artematopodidae within Elateriformia using ribosomal (18S, 28S) and mitochondrial (rrnL, cox1) molecular markers and three artematopodid species. Our analyses placed Artematopodidae close to Omethidae+Telegeusidae in a basal position of broadly defined Elateroidea. Additionally, we described the first artematopodid species from China – Eurypogon jaechi sp. nov. and E. heishuiensis sp. nov. These species are reported from mountains of Yunnan and can be easily distinguished from their Palaearctic...
Tipo: Article / Letter to the editor
Palavras-chave: MtDNA; RDNA; Diversity; Elateriformia; New species; Palaearctic Region; Phylogeny; Taxonomy; 42.75; 42.64.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/462591

Additional results and discussion to the paper: Asymmetric viability of reciprocal-cross hybrids between crested and marbled newts (Triturus cristatus and T. marmoratus)
Provedor de dados: 16
Autores: Arntzen, J.W.; Jehle, R.; Bardakci, F.; Burke, T.; Wallis, G.P.
Hybridization between divergent lineages often results in reduced hybrid viability. Here we report findings from a series of independent molecular analyses over several seasons on four life stages of F1 hybrids between the newts Triturus cristatus and T. marmoratus. These two species form a bimodal hybrid zone of broad overlap in France, with F1 hybrids making up about 4% of the adult population. We demonstrate strong asymmetry in the direction of the cross, with one class (cristatus-mothered) making up about 90% of F1 hybrids. By analyzing embryos and hatchlings, we show that this asymmetry is not due to prezygotic effects, as both classes of hybrid embryos are present at similar frequencies, implicating differential selection on the two hybrid classes...
Tipo: Article / Letter to the editor
Palavras-chave: Allozyme; Bateson–Dobzhansky–Muller incompatibility; Cytonuclear incompatibility; Hybridization; Microsatellite; MtDNA; Postzygotic isolation asymmetry; Triturus; 42.82.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/289893

Low genetic variability in the endangered Colombian endemic freshwater turtle Podocnemis lewyana (Testudines, Podocnemididae)
Provedor de dados: 16
Autores: Vargas-Ramírez, M.; Chiari, Y.; Castaño-Mora, O.V.; Menken, S.B.J.
The Magdalena River Turtle (Podocnemis lewyana) is a Colombian endemic species, endangered due to human exploitation and habitat destruction. To date, this species is poorly known ecologically and data on its genetic diversity are lacking. Here we report on the first genetic survey of the species across its distribution range. We obtained mitochondrial DNA sequences (488 bp) of the cytochrome b gene from 109 individuals. Samples belong to populations located at several different localities, grouped in five regions, along the four main river basins: Magdalena, Cauca, San Jorge, and Sinú drainages. We found two haplotypes, which differ in only one nucleotide substitution and which are represented with different frequencies in the five geographic regions....
Tipo: Article / Letter to the editor
Palavras-chave: Reptilia; Genetic population structure; Cytochrome b; MtDNA; Podocnemis; 42.82.
Ano: 2007
URL: http://www.repository.naturalis.nl/record/217421

Geographic variation and taxonomy of crested newts (Triturus cristatus superspecies): morphological and mitochondrial DNA data
Provedor de dados: 16
Autores: Arntzen, J.W.; Wallis, Graham P.
Within the newt genus Triturus, the large-bodied species in the T. cristatus (crested newt) superspecies show an unusual degree of variation in relative trunk length as a result of among-taxon variation in interlimb vertebral count. Here we examine the systematic value of this feature as assessed by both exterior measurement (Wolterstorff Index) and direct radiographic count of rib-bearing vertebrae, with particular reference to a number of confounding factors (sex differences, hybridisation, geographic variation, allometry, preservation effects). Using our mtDNA haplotype data, which are largely concordant with geographic distribution of species, we find that direct count of the rib-bearing vertebrae performs more reliably (14% misclassification) than...
Tipo: Article / Letter to the editor
Palavras-chave: Asymmetric hybridisation; Biogeography; Crested newt; Geographic variation; Morphology; MtDNA; Taxonomy; Triturus cristatus.
Ano: 1999
URL: http://www.repository.naturalis.nl/record/534387

Mitochondrial DNA polymorphism among populations of Meliponaquadrifasciata quadrifasciata Lepeletier (Apidae: Meliponini) from southern Brazil
Provedor de dados: 82
Autores: Torres,Rogelio R; Arias,Maria C; Moretto,Geraldo.
The geographical distribution of the Brazilian endemic stingless bee Melipona quadrifasciata quadrifasciata Lepeletier ranges from Rio Grande do Sul to Minas Gerais states. The objective of the present study was to verify mtDNA polymorphisms among samples of M. q. quadrifasciata collected in southern Brazil. Twenty nine colonies from three localities (Blumenau and Mafra/SC and Prudentópolis/ PR) were sampled. Seven mtDNA regions were amplified and further digested with 15 restriction enzymes (PCR-RFLP). Five composite haplotypes were identified, with two unique to samples from Prudentópolis and the remaining three to samples from Mafra and/or Blumenau.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; PCR-RLFP; Stingless bee; Haplotype.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2009000200007

Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini)
Provedor de dados: 82
Autores: Moretto,Geraldo; Arias,Maria C.
The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004

Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae)
Provedor de dados: 83
Autores: Pazian,Marlon Felix; Pereira,Luiz Henrique Garcia; Shimabukuru-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and molecular analyses were carried out in fish representative of the genus Piabina. This study specifically involved the species P. argentea and P. anhembi collected from areas of the Paranapanema and Tietê River basins, Brazil. Our findings suggest that fish classified as Piabina argentea in the Paranapanema and Tietê Rivers may represent more than one species. The samples analyzed differed by cytogenetic particularities and molecular analyses using partial sequences of the genes COI and CytB as genetic markers revealed three distinct groups of P. argentea with genetic distances sufficient to support the conclusion that the three samples analyzed are three distinct taxonomic units.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA Barcoding; Freshwater fishes; Fish; MtDNA; 18S rDNA; 5S rDNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000200008

The most important fishery resource in the Amazon, the migratory catfish Brachyplatystoma vaillantii (Siluriformes: Pimelodidae), is composed by an unique and genetically diverse population in the Solimões-Amazonas River System
Provedor de dados: 83
Autores: Formiga,Kyara Martins; Batista,Jacqueline da Silva; Alves-Gomes,José Antônio.
ABSTRACT The migratory catfish Brachyplatystoma vaillantii is one of the most important fishery resources in the Amazon. Intense capture occurs associated to its life cycle. In order to know the genetic status, we sequenced the mitochondrial DNA control region from 150 individuals of B. vaillantii, collected in five fishing landing locations, covering the length of the Solimões-Amazonas River in Brazil. Genetic diversity parameters suggest there is no genetic differentiation between the five localities. Population’s expansion indicated by R  2 and Fu’s Fs tests was also confirmed by the high number of unique haplotypes found. The Analyses of molecular variance indicated that nearly all variability was contained within locations (99.86%), and estimates of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Control region; Fishery resources; MtDNA; Piramutaba; Population genetics.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100213

Lack of mtDNA differentiation among hamlets (Hypoplectrus, Serranidae).
Provedor de dados: 20
Autores: García-Machado, E.; Chevalier, P.; Solignac, M.
The hamlets are a group of vividly colored fish species of the Serranidae family differentiated only by the color pattern of the body. Although there are divergent views about hamlet taxonomy, experimental and field observations have shown a strong assortative mating, justifying a species status for the different color morphs. Here we analyze the level of evolutionary divergence among six species in respect of mitochondrial DNA, with a view to contrasting the pattern observed with color partitions and previous results obtained with isozymes. The estimated molecular distance among species was low and of the same magnitude as nucleotide diversity within species. Consequently, the net distance and hence the time of divergence between taxa was virtually zero...
Tipo: Journal Contribution
Palavras-chave: MtDNA; Marine fish; Genetic diversity.
Ano: 2003
URL: http://hdl.handle.net/1834/4569

Diversidad haplotípica en el manatí Trichechus manatus en Cuba:  Resultados preliminares
Provedor de dados: 20
Autores: Hernández Martínez, D.; Álvarez-Alemán, A.; Bonde, R. K.; Powell, J.A.; García-Machado, E.
El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN  mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13  individuos, 12 de Cuba y uno procedente de la Florida, EU.  Las secuencias de un fragmento de 410 pb de la región de control  del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la  muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue  mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como  endémico de Belice, se encontró  en un individuo...
Tipo: Journal Contribution
Palavras-chave: Genetic diversity; MtDNA.
Ano: 2013
URL: http://hdl.handle.net/1834/5252

MOLECULAR IDENTIFICATION OF SIX SPECIES OF CALLIPHORIDAE (DIPTERA) WITH FORENSIC INTEREST IN BOGOTÁ, COLOMBIA
Provedor de dados: 84
Autores: MÁRQUEZ-ACERO,ANGELA SABRINA; VIDAL-GARCIA,JUAN MANUEL; GALINDO,LUIS FRANCISCO BECERRA; GARCÍA,ALEXANDER GARCÍA.
ABSTRACT Taxonomic identification of the species involved in the processes of cadaveric decomposition is a fundamental procedure in forensic entomological analysis. Among the species involved in the processes of decay, those of the Calliphoridae family are particularly important because they come to the body in the early stages of decomposition. The aim of this research is to identify six species of Calliphoridae (Calliphora nigribasis, Calliphora vicina, Compsomyopsis verena, Sarconesiopsis magellanica, Chrysomia albiceps and Roraimomusca roraima) with forensic interest found in Bogotá. For that, sequences of 599 bp from mitochondrial gene COII wereas obtained. The identification was made by analysis of genetic distances under Jukes-Cantor model. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Forensic entomology; Barcode; MtDNA; COII.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0031-10492017001200141

Identificação morfológica e molecular de Helicoverpa armigera (Lepidoptera: Noctuidae) e ampliação de seu registro de ocorrência no Brasil
Provedor de dados: 86
Autores: Specht,Alexandre; Sosa-Gómez,Daniel Ricardo; Paula-Moraes,Silvana Vieira de; Yano,Silvia Akimi Cavaguchi.
O objetivo deste trabalho foi descrever métodos para a caracterização morfológica e molecular de Helicoverpa armigera e ampliar o registro de ocorrência da praga no Brasil. As mariposas foram obtidas de lagartas coletadas nas culturas de algodão, milho e soja, com uso de armadilhas luminosas. As coletas foram realizadas na Bahia, no Distrito Federal, no Mato Grosso e no Paraná. A identificação foi baseada na genitália masculina e nas análises das sequências dos genes mitocondriais do citocromo B e da região cox1-tRNALeu-cox2. A genitália masculina foi comparada com as descrições morfológicas na literatura, e as sequências de genes, com as depositadas no GenBank. Ambas as análises confirmaram a presença de H. armigera nos locais de coleta. Ampliou-se o...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Código de barras de DNA; Lagarta polífaga; MtDNA; Praga quarentenária.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000600015

Genetic Diversity of Mitochondrial DNA of Chinese Black-bone Chicken
Provedor de dados: 89
Autores: Zhang,T; Du,W; Lu,H; Wang,L.
ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese Black-bone Chicken; MtDNA; D-loop region; Haplotype; Maternal origin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300565

Relationship between the genetic structure of the Andean toad Rhinella spinulosa (Anura: Bufonidae) and the northern Chile landscape (21°- 24° S)
Provedor de dados: 97
Autores: GALLARDO,CAROLINA E; HERNÁNDEZ,HÉCTOR J; DINIZ-FILHO,JOSÉ A. F; PALMA,R. EDUARDO; MÉNDEZ,MARCO A.
We analyzed the relationship of landscape and environmental features on the genetic differentiation of Rhinella spinulosa (Wiegmann, 1834) in the Altiplano of Antofagasta (Chile). We performed three types of analyses at different spatial scales: (1) Considering all populations; (2) Grouping populations by watershed and by sub-watershed; and (3) Using the results of a spatial analysis of molecular variation (SAMOVA). Landscape features were incorporated using Geographic Information Systems, with three hypothetical dispersal models: (1) Euclidean distance (null model); (2) Least cost based on wetland locations; and (3) Least cost based on least slopes. We also included differences in temperature, precipitation and altitude among localities. The Akaike...
Tipo: Journal article
Palavras-chave: Altiplano; Amphibian; Landscape genetics; Least cost models; MtDNA.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2011000300007

The genus Basilichthys (Teleostei: Atherinopsidae) revisited along its Chilean distribution range (21° to 40° S) using variation in morphologyand mtDNA
Provedor de dados: 97
Autores: VÉLIZ,DAVID; CATALÁN,LAURA; PARDO,RODRIGO; ACUÑA,PATRICIO; DÍAZ,ANGIE; POULIN,ELIE; VILA,IRMA.
There is still doubt as to the number of species of the freshwater Chilean ichthyofauna, 64 % of which have conservation problems. One of the groups is that of the silversides of the genus Basilichthys. Three morphological species of this genus have been described in Chile with disjoint distributions: Basilichthys semotilus, B. microlepidotus and B. australis; the latter two overlap in distribution only in the Aconcagua River and are not easily distinguishable by morphological and meristic characters. In order to evaluate the efficacy of identification of these species by molecular techniques, we analyzed the sequence of 9 % of the mitochondrial DNA (Control Region and COI) of individuals from the Loa River (21°41' S) to the Valdivia River (39°50' S),...
Tipo: Journal article
Palavras-chave: COI; Control Region; Drainages; MtDNA; Silverside fish.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2012000100004

Molecular variants in populations of Bryconamericus aff. iheringii (Characiformes, Characidae) in the upper Paraná river basin - doi: 10.4025/actascibiolsci.v35i2.11451
Provedor de dados: 3
Autores: Mazeti, Camila Montoro; Universidade Estadual de Maringá; Maniglia, Thiago Cintra; Universidade Estadual de Maringá; Prioli, Sônia Maria Alves Pinto; Universidade Estadual de Maringá; Prioli, Alberto José; Universidade Estadual de Maringá.
There are evidences that Bryconamericus aff. iheringii represents a species complex. DNA molecular markers have been effective in studies on phylogeny, taxonomy, and identification of cryptic species. In this study, partial sequences of genes of ATPase 6 and 8 were used to assess genetic diversity within and among populations of B. aff. iheringii of sub-basins of Tibagi, Pirapó and Ivaí rivers, belonging to the Upper Paraná river basin. The analysis of the sequences of genes pointed out high genetic diversity in B. aff. iheringii from the sub-basins studied with genetic distance values comparable to those found among different species. There was a division of the individuals into five groups. The comparison with other species of Bryconamericus that have...
Palavras-chave: Characiformes; Bryconamericus; MtDNA; Genes of ATPase 6 and 8 Biologia Molecular Characiformes; Bryconamericus; MtDNA; ATPase 8 e 6 Biologia Molecular.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/11451

Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate
Provedor de dados: 42
Autores: Almeida,Andréa M.; Bertoncini,Clélia R.A.; Borecký,Jiri; Souza-Pinto,Nadja C.; Vercesi,Aníbal E.
Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Iron; Lipid peroxidation; Mitochondria; MtDNA; Oxidative stress.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300010

Wide-range genetic connectivity of Coney, Cephalopholis fulva (Epinephelidae), through oceanic islands and continental Brazilian coast
Provedor de dados: 42
Autores: Souza,Allyson S. de; Dias Júnior,Eurico A.; Galetti Jr,Pedro M.; Machado,Erik G.; Pichorim,Mauro; Molina,Wagner F.
The Epinephelidae form a group of species of high biological and economical interests. It´s phylogeographic patterns are not well known especially the distributed populations in the western region of the Atlantic Ocean. Among the representatives is a small species called Cephalopholis fulva, Coney, which presents a wide geographical distribution, polychromia, hermaphroditism and is quickly becoming a large target for the exploration of commercial fishing. The genetic and historical demography were obtained through the partial sequence analysis of Control Region from six locations on the coastline of Brazil from the northeast coast to the southwest coast, including the oceanic islands of Rocas Atoll and Fernando de Noronha Archipelago. The analyzed samples...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: D-loop; Glaciation; MtDNA; Panmixia; Phylogeography.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100121

Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn)
Provedor de dados: 48
Autores: Lakra,Wazir S; Goswami,M; Rajaswaminathan,T; Rathore,Gaurav.
Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100...
Tipo: Journal article
Palavras-chave: Cyprinus carpio; Cell line; MtDNA.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400002

Insights into the genetic diversity of the leopard seal (Hydrurga leptonyx), inferred from mitochondrial DNA analysis, at Danco Coast, Antarctic Peninsula
Provedor de dados: 50
Autores: Hernández-Ardila,Laura Valentina; Barragán-Barrera,Dalia C.; Negrete,Javier; Poljak,Sebastián; Riet-Sapriza,Federico G.; Caballero,Susana.
ABSTRACT The leopard seal (Hydrurga leptonyx) is a genetically low-studied species. In order to conduct the first genetic diversity assessment of this species in Danco Coast (Antarctic Peninsula), 423 bp of the mitochondrial DNA Control Region (mtDNA-CR) was sequenced from 13 blood samples collected in Primavera Base (62º15’S, 58º39’W) during the 2011-2012 austral summer. Our results showed high haplotype diversity (h = 0.99), with various divergent haplotypes. Our findings suggest that leopard seals in the Danco Coast could represent different phylogroups; however, including more genetic markers are needed to confirm this hypothesis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Seal; Pinnipeds; MtDNA; Control Region; Antarctica.
Ano: 2021
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300227

Genetic variability of brycon orbignyanus (valenciennes, 1850) (characiformes: characidae) in cultivated and natural populations of the upper paraná river, and implications for the conservation of the species
Provedor de dados: 52
Autores: Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria.
This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025

Mitochondrial control region haplotypes of the South American sea lion Otaria flavescens (Shaw, 1800)
Provedor de dados: 56
Autores: Artico,L.O.; Bianchini,A.; Grubel,K.S.; Monteiro,D.S.; Estima,S.C.; de Oliveira,L.R.; Bonatto,S.L.; Marins,L.F.
The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Otaria flavescens; MtDNA; D-loop; Genetic variability; New haplotypes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900002

Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism
Provedor de dados: 56
Autores: Hilsdorf,A.W.; Krieger,J.E.
Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Restriction fragment length polymorphism (RFLP); Rattus norvegicus; MtDNA; Laboratory rats; Genetic monitoring.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003

Maternal inheritance on reproductive traits in Brangus-Ibagé cattle
Provedor de dados: 65
Autores: Henkes,Luis Ernani; Benavides,Magda Vieira; Oliveira,João Francisco Coelho; Moraes,José Carlos Ferrugem; Weimer,Tania Azevedo.
Cytoplasmic inheritance influence on reproductive traits was investigated in the Brangus-Ibagé cattle (3/8 Nelore x 5/8 Aberdeen Angus). Additive genetic effects were responsible for 12% ± 11% of phenotypic variation observed in first calving interval, but their contribution dropped to zero when all calving intervals (CI) were considered. The heritability estimate for age at first calving (AFC, in days) was 0.19 ± 0.09. Mitochondrial lineage (MIT) had negligible effects on phenotypic variances of calving interval (0.0 ± 0.02), calf birth weight (0.0 ± 0.01), and cow weight at calving (0.0 ± 0.01). However, for the age at first calving, MIT accounted for 0.15 ± 0.07 of total variation. Cow weight at calving had a significant linear effect on CI and AFC....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Maternal inheritance; Calving interval.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400030