Consulta: subjectFacets:"Murine embryonic stem cell"
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Data/hora: 09/06/2024 18:49:44

Single embryoid body formation in a multi-well plate
Provedor de dados: 69
Autores: Ezekiel,Uthayashanker R; Muthuchamy,Mariappan; Ryerse,Jan S; Heuertz,Rita M.
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells recapitulate many aspects of a developing embryo. Of specific importance, synchronous differentiation of EB recapitulates organ-specific development and is achieved in culture by formation of uniformly sized EB. The method described here demonstrates a simple and cost-effective way of generating EB from murine ES cells. Single EB are formed in a multi-well plate format and large numbers of EB are generated using a 96-well multi-well plate. Uniform single-sized EB formed in the multi-well are an ideal system for screening compounds and determining differentiation effects. Since EB contain all three germ layers, they are appropriate for studying small molecule effects on differentiation of ES...
Tipo: Journal article
Palavras-chave: Differentiation; EB; ES; Murine embryonic stem cell.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200015