Consulta: subjectFacets:"Mycobacterium tuberculosis"
Registros recuperados: 57
Data/hora: 02/06/2024 07:37:46

Diagnóstico de tuberculose bovina: avanços na identificação de Mycobacterium bovis por espectrometria de massas MALDI-TOF.
Provedor de dados: 14
Autores: BACANELLI, G. M.; OLARTE, L. C.; LEGUIZAMON, G. O. de C.; SILVA, M. R.; RODIGUES, R. A.; PASQUATTI, T. N.; CARNEIRO, P. A.; ETGES, R. N.; ARAUJO, F. R.; VERBISCK, N. V.
bitstream/item/215558/1/COT-146-Final-em-Alta.pdf
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Espectrometria de massa; Mycobacterium tuberculosis.
Ano: 2020
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1124539

Pulmonary tuberculosis: hematology, serum biochemistry and the relation with the disease duration
Provedor de dados: 80
Autores: Oliva,V. M.; Cezário,G. A. G.; Cocato,R. A.; Marcondes-Machado,J.
The aim of the present study was to analyze the relationship between hematological and biochemical parameters and tuberculosis process activity time according to clinical complaint duration. It was a retrospective study analyzing medical records from 80 pulmonary tuberculosis patients at Botucatu Medical School University Hospital (Botucatu, São Paulo State, Brazil), who were divided into 2 groups according to clinical complaint duration: Group 1 (G1) - up to three months; Group 2 (G2) - over three months. Parameters included: age, gender, bacilloscopy, erythrocyte sedimentation rate (ESR), platelet count, alpha1-globulin, alpha2-globulin, gamma globulin, mucoprotein, alpha1-acid glycoprotein values, and the presence of risk factors such as smoking,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Pulmonary tuberculosis; Smoking; Hematolological and biochemical parameters; Erythrocyte sedimentation rate.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000100006

PKO: alternative method for isolating mycobacteria from sputum
Provedor de dados: 36
Autores: Salem,Julia Ignez; Carvalho,Clarice Maia; Ogusku,Mauricio Morishi; Maia,Rosália; Ruffino-Netto,Antonio.
We elaborated an alternative culture method, which we denominated PKO (initials in tribute of respect to Petroff, Kudoh and Ogawa), for isolating Mycobacterium tuberculosis from sputum for diagnosis of pulmonary tuberculosis (TB), and to compare its performance with the Swab and Petroff methods. For the technique validation, sputum samples from patients suspected of pulmonary TB cases were examined by acid-fast microscopy (direct and concentrated smear), PKO, Swab and Petroff methods. We found that Petroff and PKO methods have parity in the effectiveness of M. tuberculosis isolation. However, by the PKO method, 65% of isolated strains were detected in a period of &lt;FONT FACE=Symbol&gt;£&lt;/FONT&gt;15 days, while by the Petroff method the best detection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diagnostic method; Mycobacterium tuberculosis; PKO; Tuberculosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672007000300013

THE HUMAN MICROBIOTA: THE ROLE OF MICROBIAL COMMUNITIES IN HEALTH AND DISEASE
Provedor de dados: 37
Autores: BOTERO,Luz Elena; DELGADO-SERRANO,Luisa; CEPEDA HERNÁNDEZ,Martha Lucía; DEL PORTILLO OBANDO,Patricia; ZAMBRANO EDER,María Mercedes.
During the last decade, there has been increasing awareness of the massive number of microorganisms, collectively known as the human microbiota, that are associated with humans. This microbiota outnumbers the host cells by approximately a factor of ten and contains a large repertoire of microbial genome-encoded metabolic processes. The diverse human microbiota and its associated metabolic potential can provide the host with novel functions that can influence host health and disease status in ways that still need to be analyzed. The microbiota varies with age, with features that depend on the body site, host lifestyle and health status. The challenge is therefore to identify and characterize these microbial communities and use this information to learn how...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 16S rRNA gene; Microbial diversity; Microbiome; Mycobacterium tuberculosis.
Ano: 2016
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000100001

Meta-análisis: diagnóstico de tuberculosis resistente por técnicas no comerciales de amplificación
Provedor de dados: 38
Autores: Imperiale,Belén Rocío.
La emergencia de tuberculosis (TB) multidrogo y extensivamente-resistente reactivó la necesidad de contar con métodos rápidos para detectar resistencia a isoniacida (INH) y rifampicina (RIF). Por tal motivo, los objetivos de este trabajo fueron evaluar, mediante meta-análisis, la exactitud global y la posible utilidad de métodos caseros basados en PCR para la detección rápida de resistencia a INH y RIF en aislamientos clínicos de Mycobacterium tuberculosis. La búsqueda bibliográfica incluyó Medline/PubMed, BioMedLib. Para estimar la variabilidad entre los resultados de los estudios y el grado de exactitud diagnóstica de los métodos utilizados se realizaron gráficos "forest plot" y curvas SROC (summary receiver operating characteristic) mediante el software...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Sensibilidad a drogas; Rifampicina; Isoniacida; Métodos moleculares; Reacción en cadena de la polimerasa; Métodos rápidos.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572013000200017

Natural Compounds from Mexican Medicinal Plants as Potential Drug Leads for Anti-Tuberculosis Drugs
Provedor de dados: 42
Autores: GÓMEZ-CANSINO,ROCIO; GUZMÁN-GUTIÉRREZ,SILVIA LAURA; CAMPOS-LARA,MARÍA GUADALUPE; ESPITIA-PINZÓN,CLARA INES; REYES-CHILPA,RICARDO.
ABSTRACT In Mexican Traditional Medicine 187 plant species are used in the treatment of respiratory conditions that may be associated with tuberculosis. In this contribution, we review the ethnobotany, chemistry and pharmacology of 63 species whose extracts have been assayed for antimycobacterial activity in vitro. Among these, the most potent is Aristolochia brevipes (MIC= 12.5 µg/mL), followed by Aristolochia taliscana, Citrus sinensis, Chrysactinia mexicana, Persea americana, and Olea europaea (MIC&lt;64 µg/mL). Other potent extracts (inhibition &gt; 95%, 50 µg/mL) include: Amphipterygium adstringens, Larrea divaricata, and Phoradendron robinsoni. Several active compounds have been identified, the most potent are: Licarin A (isolated from A. taliscana),...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Medicinal plants; Natural products; Mycobacterium tuberculosis.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100031

Retrospective study of 668 cultures for mycobacteria in a reference hospital for AIDS in Southern Brazil
Provedor de dados: 55
Autores: Pasqualotto,Alessandro Comarú; Rosa,Daniela Dornelles; Pereira,Maria do Carmo Fontoura; Targa-Ferreira,Roberto L.; Santos,Breno Riegel.
Although AIDS patients in other countries are frequently diagnosed as having atypical mycobacterial infection, in Southern Brazil there is a clinical impression that Mycobacterium tuberculosis is the rule rather than the exception. We made a retrospective review of cultures for mycobacteria at our hospital in order to determine the frequency of atypical mycobacteria and Mycobacterium tuberculosis in hospitalised patients in Porto Alegre, Brazil. Mycobacterium tuberculosis was the most frequent isolate (79.9%), regardless of HIV serostatus. Only 1.5% of the cultures yielded atypical mycobacteria, all of which in AIDS patients. Mycobacterium tuberculosis was diagnosed in most of the HIV-infected patients (81.2%). We conclude that Mycobacterium tuberculosis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; AIDS; Southern Brazil.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200005

Spinal epidural abscess due to Mycobacterium tuberculosis in a patient with AIDS: case report and review of the literature
Provedor de dados: 55
Autores: Metta,Humberto; Corti,Marcelo; Redini,Liliana; Yampolsky,Claudio; Schtirbu,Ricardo.
Spinal epidural abscess (SEA) is a rare infectious disorder that often has delayed diagnosis and is associated with significant morbidity and mortality rates. We present a case of an AIDS patient with a SEA due to Mycobacterium tuberculosis. This type of SEA in AIDS patients is characterized by localized spinal pain and prolonged fever. Magnetic resonance imaging is the method of choice in the diagnostic process. Early diagnosis, followed by specific therapy (surgical decompression combined with antituberculous drugs), is necessary to improve the prognosis of these kinds of patients.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Spinal epidural abscess; Mycobacterium tuberculosis; AIDS.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000200013

Multidrug-resistant tuberculosis: case reports study in a central state of Brazil
Provedor de dados: 55
Autores: Araújo-Filho,João Alves de; Vasconcelos-Jr,Arioldo Carvalho; Sousa,Eduardo Martins de; Silveira,Colombina da; Sousa,Patrícia Tavares Pereira de; Severo,Klaus Andrade; Vieira,Ludmila Ferreira; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Multidrug-resistant tuberculosis (MDR-TB) is an emerging and worrisome health problem that threatens tuberculosis (TB) control worldwide. The clinical management of MDR-TB is a complex issue associated with the use of multiple drugs for a long period, usually accompanied by side effects and high costs. The objective of this work was to relate cases of MDR-TB occurring in Goiás, a central state of Brazil. We related five cases of MDR-TB, three women and two men. All were pulmonary cases. Three were in their second treatment and two in their first treatment. Surgical pulmonary resection was performed in one case. One death occurred. Lack of adherence, gastric intolerance to anti-TB drugs and poor clinical management were the main aspects related to the...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Tuberculosis; Multidrug-resistant tuberculosis; MDR-TB; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100020

CD4+ cell counts in patients with different clinical manifestations of tuberculosis
Provedor de dados: 55
Autores: Davoudi,Setareh; Rasoolinegad,Mehrnaz; Younesian,Masoud; Hajiabdolbaghi,Mahboubeh; Soudbakhsh,Abdolreza; Jafari,Sirous; EmadiKouchak,Hamid; Mehrpouya,Morteza; Lotfi,Hossein.
Tuberculosis is the prototype of infections that require a cellular immune response for their control. It has been shown that CD4+ T-lymphocytes are most important in the protective response against Mycobacterium tuberculosis. CD8+ T-lymphocytes are also important for effective T-cell immune response. This study compares CD4+ and CD8+ baseline values in patients with different manifestations of tuberculosis. CD4+ and CD8+ in three groups of patients with tuberculosis (pulmonary, lymphadenitis, meningitis/milliary involvement) and a group of healthy volunteers were enumerated using flowcytometry. Twenty-six patients with pulmonary tuberculosis, 10 with adenitis, 16 with meningitis or milliary tuberculosis and 16 healthy volunteers entered the study. Mean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; CD4.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600008

Profound peripheral T-lymphocyte depletion and activation in disseminated tuberculosis
Provedor de dados: 55
Autores: Rodrigues,Denise Silva; Salomão,Reinaldo; Kallas,Esper Georges.
Three HIV-1-seronegative patients with disseminated tuberculosis presented significant depletion of T-cell counts, in CD4+ and/or CD8+ cells, associated with increased expression of activation marker CD38 on CD8+ T-lymphocytes. This finding raises the question of potential mechanisms involved in the activation or loss of T-cells in disseminated tuberculosis.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium tuberculosis; Disseminated tuberculosis; Lymphocyte; Cellular activation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100012

Evaluation of the role of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer of epithelial cells
Provedor de dados: 55
Autores: Ramsugit,Saiyur; Pillay,Balakrishna; Pillay,Manormoney.
Abstract This study was undertaken in order to assess the involvement of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer in the M. tuberculosis-epithelial cell interaction. A MTP-deficient strain of M. tuberculosis demonstrated a significant reduction of 69.39% (p = 0.047) and 56.20% (p = 0.033) in its ability to adhere to and invade A549 pulmonary epithelial cells, respectively, in comparison with the wild-type strain. Complementation of the MTP-deficient mutant restored its adhesion and invasion capacity back to the wild-type levels. Overall, it was found that similar concentrations of IL-1β, IL-4, IL-6, IL-8, G-CSF, IFN-γ, MCP-1, and TNF-α were induced in A549 cells infected with the MTP-proficient and MTP-deficient...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Curli pili; Adhesin; Invasin.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200160

Tuberculosis: a study of 111 cases in an area of high prevalence in the extreme south of Brazil
Provedor de dados: 55
Autores: Mattos,Ivo Gomes de; Ribeiro,Marta Osório; Netto,Isabel Cristina de O.; d'Azevedo,Pedro Alves.
Tuberculosis is an increasingly important public health problem in developing countries. We studied 111 tuberculosis patients confirmed by mycobacteria isolation between 1998 and 2000 in Rio Grande, in south Brazil. One-hundred-thirteen pulmonary and extrapulmonary clinical materials were examined through culture by the Ogawa-Kudoh method and through smear examination by the Ziehl-Nielsen or Kinyoun methods. The identification of Mycobacterium tuberculosis was done through the usual phenotypical methods. The proportion method (indirect technique) was used to determine the resistance of isolates. The man/woman ratio was 2.6:1, 75% were white, and the group had a mean age of 39.7±12.7. Risk factors were present in 38.7% (34 men and 9 women, p=0.2), with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; Resistance.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000300008

Extensively drug-resistant tuberculosis: a case report and literature review
Provedor de dados: 55
Autores: Araújo-Filho,João Alves de; Vasconcelos-Jr,Arioldo Carvalho; Sousa,Eduardo Martins de; Silveira,Colombina da; Ribeiro,Elisangela; Kipnis,André; Junqueira-Kipnis,Ana Paula.
Extensively drug-resistant tuberculosis (XDR-TB) is an emerging health problem that threatens tuberculosis (TB) control worldwide, since suitable treatment for this disease has not yet been found. We report a case of secondary pulmonary XDR-TB in a 54-year-old, HIV-negative male from Goiânia, Brazil. The patient had long-standing pulmonary tuberculosis (nine years) with extensive bilateral lung damage and had been treated with multiple antituberculosis drugs (self-administered) before XDR-TB diagnosis. The strain of Mycobacterium tuberculosis was resistant to R- rifampicin, H-isoniazid, E-ethambutol, Eto-ethionamide, Ofx-ofloxacin, and Am-amikacin. This patient died with multiple organ failure due to sepsis secondary to bacterial pneumonia.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Extensively drug-resistant tuberculosis; XDR-TB; Tuberculosis; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500019

Anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from patients in a tertiary hospital in Bahia
Provedor de dados: 55
Autores: Matos,Eliana Dias; Lemos,Antônio Carlos Moreira; Bittencourt,Carolina; Mesquita,Cristiane Leite.
Anti-tuberculosis drug resistance, particularly multiresistance, is a crucial issue in the control of tuberculosis (TB). This study estimated the prevalence of primary and acquired anti-tuberculosis drug resistance in strains of Mycobacterium tuberculosis isolated from hospitalized patients, to identify the risk factors for resistance, and to evaluate the its impact on hospital mortality for tuberculosis. Strains of Mycobacterium tuberculosis from 217 patients hospitalized for TB were analyzed. Subjects were recruited sequentially at a TB reference hospital in Salvador, Bahia, Brazil from July 2001 to July 2003. Multiresistant (MR) strains were defined as strains resistant to rifampicin and isoniazid. Of a total of 217 strains isolated, 41 (19.0%, 95%CI:...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Resistance; Multiresistance; Hospitals; Bahia-Brazil.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000300007

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil
Provedor de dados: 55
Autores: Moreira-Oliveira,M.S.; Oliveira,H.B.; Pace,F.; Stehling,E.G.; Rocha,M.M.M.; Aily,D.C.G.; Brocchi,M.; Silveira,W.D.
The objective of this study was to investigate the possible transmission of tuberculosis among 39 inmates with positive Mycobacterium tuberculosis smears in four correctional institutions located in Campinas City, SP, Brazil over a 19-month period. Fifty-one M. tuberculosis isolates from these inmates were characterized according to the number of IS6110 insertion elements present in their genomic DNA. The number of insertion elements in M. tuberculosis isolates varied from two to twelve. The dendrogram of similarity resulted in the grouping the isolates in six main clusters. These results, associated to epidemiological data, suggested the transmission of tuberculosis among inmates of the same and different institutions inmates. Univariate analysis of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Transmission; Molecular genotyping; Campinas; SP; Brazil.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600009

Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes
Provedor de dados: 55
Autores: Café Oliveira,Luita Nice; Muniz-Sobrinho,Jairo da Silva; Viana-Magno,Luiz Alexandre; Oliveira Melo,Sônia Cristina; Macho,Antonio; Rios-Santos,Fabrício.
Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to reduce morbidity and medical costs. Despite the availability of several molecular-based commercial-kits to diagnose multidrug-resistant tuberculosis, their diagnostic value might diverge worldwide since Mycobacterium tuberculosis genetic variability differs according to geographic location. Here, we studied the predictive value of four common mycobacterial mutations in strains isolated from endemic areas of Brazil. Mutations were found at the frequency of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biomarkers; Diagnosis; Multidrug-resistance; Mycobacterium tuberculosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200166

Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens
Provedor de dados: 55
Autores: Hung,Hung-Chang; Chan,Chi-Ho; Tsao,Shih-Ming; Chen,Shiuan-Chih; Wu,Tzu-Chin; Lu,Min-Chi; Wu,Der-Jinn; Huang,Shu-Ju; Lee,Yuan-Ti.
OBJECTIVE: The diagnostic efficacy of the BDProbeTEC ET Mycobacterium tuberculosis (MTB) complex direct detection assay (DTB) performed on bronchoalveolar lavage (BAL) specimens and sputum smears was compared with acid-fast bacilli (AFB) smear microscopy. METHOD: AFB smear microscopy, DTB and culture results of 286 patients with pulmonary tuberculosis were retrospectively reviewed. A total of 120 patients provided expectorated sputum samples, and 166 patients provided BAL specimens. Culture results and clinical diagnosis were used as gold standards. RESULTS: The sensitivity and specificity of the DTB assay in detecting MTB in sputum specimens was significantly higher compared to AFB smear microscopy (83.7% and 82.4%, vs. 75.6%, and 41.2%, respectively)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bronchoalveolar lavage; Mycobacterium tuberculosis; Acid-fast bacilli staining; Molecular diagnosis; BDProbeTEC ET system.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300005

Characterization of gyrA and gyrB mutations and fluoroquinolone resistance in Mycobacterium tuberculosis clinical isolates from Hubei Province, China
Provedor de dados: 55
Autores: Chen,Jun; Chen,Zhifei; Li,Yuanyuan; Xia,Wei; Chen,Xi; Chen,Tian; Zhou,Liping; Xu,Bin; Xu,Shunqing.
OBJECTIVE: The study aimed to investigate gyrA and gyrB mutations in Mycobacterium tuberculosis (MTB) clinical strains from 93 patients with pulmonary tuberculosis in Hubei Province, China, and analyze the association between mutation patterns of the genes and ofloxacin resistance level. RESULTS: Among 93 MTB clinical isolates, 61 were ofloxacin-resistant by the proportion method, and 32 were ofloxacin-susceptible MDR-TB. No mutation in the gyrB gene was found in any MTB strains. In the 61 ofloxacin-resistant isolates, 54 mutations were observed in the gyrA gene. Only one mutation in the gyrA gene was found in ofloxacin-susceptible MDR-TB isolates. In this study, the mutation patterns of gyrA involved seven patterns of single codon mutation (A90V, S91P,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Fluoroquinolones; DNA mutational analysis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200005

Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India
Provedor de dados: 55
Autores: Prasad,Polu Giri; Jasmine,Mohammad Shaik; kanta,Kota Neela Mani; Deepthi,Karumanchi; Allam,Uday Sankar.
ABSTRACT  Purpose and objectives Detection of drug resistance plays a crucial role in tuberculosis (TB) treatment and prevention of Mycobacterium tuberculosis (MTB) transmission. The aim of this study was to determine the levels and patterns of resistance of MTB isolates to two key anti-TB drugs (rifampicin, RIF and isoniazid, INH) and the type of mutations in drug resistance genes (rpoB, katG and inhA) of the isolates at the southern coastal region of Andhra Pradesh, India, using commercially available GenoType MTBDRplus assay under the Revised National TB Control Program.  Methods GenoType MTBDRplus assay was performed on 2859 sputum smear-positive samples and the mutations in the genes responsible for resistance (rpoB, katG and inhA) were analyzed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Multidrug resistance; Mycobacterium tuberculosis; MTBDRplus assay; Mutations; Molecular detection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500281

Oral manifestation of tuberculosis: a case-report
Provedor de dados: 55
Autores: de Souza,Bárbara Capitanio; de Lemos,Vania Maria Aita; Munerato,Maria Cristina.
Abstract The present case-report describes tuberculosis on the oral mucosa, in a rare manifestation of the disease. The importance of appropriate diagnosis and awareness of the clinical manifestations is highlighted. Oral lesions seem to occur as chronic ulcers, nodular or granular areas, and rare, firm leukoplakia regions. Most extra-pulmonary lesions represent secondary infections of a primary lung infectious focus; therefore, early and accurate diagnosis is required for planning of the best treatment and strategies to control the disease.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Mycobacterium infection; Oral lesion; Oral tuberculosis; Mycobacterium tuberculosis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000200210

A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Provedor de dados: 55
Autores: Wang,Xia; Jiao,Junhua; Xu,Weihua; Chai,Xiaoyan; Li,Zhenyun; Wang,Qingjiang.
OBJECTIVE: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Multiplex allele specific polymerase chain reaction (MAS-PCR).
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600008

Interferon-gamma release assay versus tuberculin skin test for latent tuberculosis infection among HIV patients in Brazil
Provedor de dados: 55
Autores: Kussen,Gislene Maria Botão; Dalla-Costa,Libera Maria; Rossoni,Andrea; Raboni,Sonia Mara.
Abstract  Setting Patients HIV+ attending in a reference clinic, Southern Brazil.  Objective To compare the interferon-gamma-release assay (IGRA – QuantiFERON® TB Gold In-Tube) with the tuberculin skin test (TST – PPD-Rt 23) for latent tuberculosis infection (LTBI) in patients with HIV.  Design Cohort study. Patients were simultaneously submitted to the TST and blood collection for the IGRA.  Results A total of 140 subjects were included. Nine (6.4%) were IGRA+/TST+, 12 (8.6%) were IGRA+/TST−, 4 (3%) were IGRA−/TST+, and 115 (82%) IGRA−/TST−. There was poor agreement between tests (kappa = 0.2), and no correlation between these results and CD4+ T lymphocyte counts. During follow-up, one patient with negative results on both tests died from sepsis, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Immunological tests; Immunocompromised patients.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100069

A systematic review of East African-Indian family of Mycobacterium tuberculosis in Brazil
Provedor de dados: 55
Autores: Duarte,Tonya Azevedo; Nery,Joilda Silva; Boechat,Neio; Pereira,Susan Martins; Simonsen,Vera; Oliveira,Martha; Gomes,Maria Gabriela Miranda; Penha-Gonçalves,Carlos; Barreto,Mauricio Lima; Barbosa,Theolis.
ABSTRACT  Introduction: The Mycobacterium tuberculosis East African-Indian (EAI) spoligotyping family (belonging to lineage 1, Indo-Oceanic, defined by the region of deletion RD239) is distributed worldwide, but is more prevalent in Southeast Asia, India, and East Africa. Studies in Latin America have rarely identified EAI. In this study, we describe the occurrence of the EAI family in Brazil.  Methods: EAI was identified in a systematic literature review of genetic diversity studies pertaining to M. tuberculosis in Brazil, as well as in a survey conducted in Salvador, Bahia, located in the northeastern region of this country.  Results: The EAI6-BGD1 spoligotyping family and the EAI5 Spoligotype International Type (SIT) 1983 clade were the most frequently...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Phylogeography; Genotyping; Epidemiology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300317

Mycobacterium tuberculosis bacteremia diagnosed in an HIV-negative patient in Brazil: a rare or an under-reported event?
Provedor de dados: 55
Autores: Hadad,D. J.; Pignatari,A.C.C.; Machado,M.A.O.; Telles,M.A.S.
A case of Mycobacterium tuberculosis bacteremia in an HIV negative immunodepressed patient was described using the BACTEC 460 TB system. This bacterium should be investigated in the blood of immunodepressed non-HIV infected patients with prolonged fever.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Mycobacterium tuberculosis; Mycobacteremia; Immunodeficiency.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000200010

Determination of the activity of standard anti-tuberculosis drugs against intramacrophage Mycobacterium tuberculosis, in vitro: MGIT 960 as a viable alternative for BACTEC 460
Provedor de dados: 55
Autores: Jhamb,Sarbjit Singh; Goyal,Amit; Singh,Prati Pal.
BACTEC 460 has now been phased out, so the search for an alternative is imperative. We have determined the activity of standard anti-tuberculosis drugs against intramacrophage Mycobacterium tuberculosis, in vitro, by using BACTEC 460 and MGIT 960 methods. The minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol and streptomycin against intracellular M. tuberculosis H37Rv were found to be 0.2, 0.8, 8.0, and 5.0 µg/mL, respectively, by both methods. These results show a significant (p &lt; 0.001) concordance between minimum inhibitory concentrations obtained by these two different methods. MGIT 960 system uses a robust florescence quenching-based oxygen sensor, requires no radioisotope, is safe, and relatively easy to operate. Apparently,...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: BACTEC 460; Macrophages; MGIT 960; Mycobacterium tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300336

Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis
Provedor de dados: 55
Autores: Chen,Jiazhen; Ruan,Qiaoling; Shen,Yaojie; Wang,Sen; Shao,Lingyun; Zhang,Wenhong.
ABSTRACT The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans.In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. A pool of promising epitopes was then assessed for their diagnostic value in 233 suspected TB patients using a whole blood IFN-γ release assay.Only 10 peptides were recognized by more than 10% of active tuberculosis patients....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; T-cell epitope; Diagnostic antigen.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600462

Factors related to HIV/tuberculosis coinfection in a Brazilian reference hospital
Provedor de dados: 55
Autores: Carvalho,Bráulio Matias de; Monteiro,André Jalles; Pires Neto,Roberto da Justa; Grangeiro,Thalles Barbosa; Frota,Cristiane Cunha.
Infection with both Human Immunodeficiency Virus (HIV) and Mycobacterium tuberculosis is currently the world's leading cause of death due to infectious agents. We evaluated factors related to the development of tuberculosis (TB) in HIV-infected patients who were being treated at an infectious diseases hospital in Fortaleza, Ceará, Brazil. From January 2004 to December 2005, we made an epidemiological study through the analysis of the medical records of 171 patients, who were diagnosed as having both HIV and tuberculosis. Among these co-infected patients, most (81%, p=0.0006) were male. Co-infection was more frequent (87.8%) among patients over 40 years of age and those with lower educational levels (less than eight years of schooling). Forty-one percent of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Mycobacterium tuberculosis; HIV/TB co-infection; Risk factors; AIDS.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400005

Mycobacterium tuberculosis complex bacteremia among HIV and non-HIV patients in a Mexican tertiary care center
Provedor de dados: 55
Autores: Tamez-Torres,Karla Maria; Torres-Gonzalez,Pedro; Cervera-Hernandez,Miguel E.; Chavez-Mazari,Barbara; Barrientos-Flores,Corazon de Jesus; Bobadilla-del-Valle,Miriam; Ponce-de-Leon,Alfredo; Sifuentes-Osornio,Jose.
ABSTRACT  Objectives: To determine the factors associated with Mycobacterium tuberculosis complex-positive blood culture.  Methods: Case-control study. Sociodemographic, clinical and laboratory data were collected from 2000 to 2015.  Results: We reviewed medical records of 533 patients with culture-proven tuberculosis, of whom 27.2% (145/533) had blood culture available. Patients with mycobacteremia presented more frequently with abdominal tuberculosis, body mass index &lt;18 kg/m2, and had lower hemoglobin and albumin levels. No differences were observed regarding HIV status.  Conclusions: Few studies have reported on the characteristics associated with Mycobacterium tuberculosis complex bacteremia, especially among Human Immunodeficiency Virus-negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Human immunodeficiency virus; Bloodstream infection; Mycobacteremia; Case-control study.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500387

IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage
Provedor de dados: 55
Autores: Jee,Babban; Sharma,Pawan; Katoch,Kiran; Joshi,Beenu; Awasthi,Sudhir Kumar.
Abstract Mycobacterium tuberculosis (MTB) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. Amongst the many known factors, small heat shock protein 16.3 (sHSP16.3) of MTB encoded by gene hspX has been reported to be critical for the survival of MTB. In the present study, the effect of recombinant murine interferon-gamma (rmIFN-γ) and recombinant murine interleukin-10 (rmIL-10) on the expression of gene hspX of MTB in murine macrophage RAW264.7 has been investigated. By real-time RT-PCR, it was observed that three increasing concentrations (5, 25 and 50 ng/ml) of rmIFN-γ significantly up-regulated the expression of hspX whereas similar concentrations of rmIL-10 (5, 25 and 50 ng/ml) significantly down-regulated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; HspX; RmIFN-γ; RmIL-10; Murine macrophage.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400386

Polymerase chain reaction as a useful and simple tool for rapid diagnosis of tuberculous meningitis in a Brazilian tertiary care hospital
Provedor de dados: 55
Autores: Dora,José Miguel; Geib,Guilherme; Chakr,Rafael; Paris,Fernanda de; Mombach,Alice Beatriz; Lutz,Larissa; Souza,Carolina Fischinger Moura de; Goldani,Luciano Z.
Meningitis is a severe and potentially fatal form of tuberculosis. The diagnostic workup involves detection of acid-fast bacilli (AFB) in the cerebrospinal fluid (CSF) by microscopy or culture, however, the difficulty in detecting the organism poses a challenge to diagnosis. The use of the polymerase chain reaction (PCR) in the diagnostic approach to Mycobacterium tuberculosis (MTB) meningitis has been reported as a fast and accurate method, with several commercial kits available. As an alternative, some institutions have been developing inexpensive in house assays. In our institution, we use an in house PCR for tuberculosis. We analyzed the performance of our PCR for the diagnosis of MTB meningitis in 148 consecutive patients, using MTB culture as gold...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculous meningitis; Diagnosis; Polymerase chain reaction; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300015

Previous use of quinolones: a surrogate marker for first line anti-tuberculosis drugs resistance in HIV-infected patients?
Provedor de dados: 55
Autores: Deutschendorf,Caroline; Goldani,Luciano Z.; Santos,Rodrigo Pires dos.
OBJECTIVES: Drug resistant Mycobacterium tuberculosis causes much higher rates of treatment toxicity, failure or relapse, and mortality. We determined the drug resistant profile of Mycobacterium tuberculosis strains isolated from a population of HIV-infected patients in southern Brazil and studied the potential factors associated with resistance. METHODS: We conducted a retrospective cohort study to determine the resistance profile of Mycobacterium tuberculosis isolated from HIV-infected patients and factors that could be associated with resistance from 2000 to 2005. RESULTS: 236 patients were included in the study. Resistance to at least one drug was observed in 32 (14.6%) isolates, and multi-drug resistance was observed in 4 (1.82%) isolates. On...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; HIV; Quinolones; Drug resistance.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200006

Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity
Provedor de dados: 56
Autores: Matsui,T.; Carneiro,C.R.W.; Leão,S.C.
The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003

Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system
Provedor de dados: 56
Autores: Bigi,F.; Taboga,O.; Romano,M.I.; Alito,A.; Fisanotti,J.C.; Cataldi,A.A.
In the present study we evaluated different systems for the expression of mycobacterial antigen P36 secreted by Mycobacterium bovis. P36 was detected by Western blot using a specific antiserum. The P36 gene was initially expressed in E. coli, under the control of the T7 promoter, but severe proteolysis prevented its purification. We then tried to express P36 in M. smegmatis and insect cells. For M. smegmatis, we used three different plasmid vectors differing in copy number and in the presence of a promoter for expression of heterologous proteins. P36 was detected in the cell extract and culture supernatant in both expression systems and was recognized by sera from M. bovis-infected cattle. To compare the expression level and compartmentalization, the MPB70...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Mycobacterium smegmatis; Insect cells; Baculovirus; Secreted proteins; P36; MPB70; Antigen.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100004

Phenotypic and genotypic variant of MDR-Mycobacterium tuberculosis multiple isolates in the same tuberculosis episode, Rio de Janeiro, Brazil
Provedor de dados: 56
Autores: Andrade,M.K.N.; Machado,S.M.A.; Leite,M.L.; Saad,M.H.F.
Assuming that the IS6110-restriction fragment length polymorphism (RFLP) changes at a constant rate of 3.2 years, this methodology was applied to demonstrate, for the first time, variant patterns of Mycobacterium tuberculosis (MTB) in multiple isolates obtained at short time intervals from sputum and blood of an HIV+ patient with multiple admissions to the Emergency Room and to the multidrug-resistant tuberculosis (MDR-TB) Reference Center of a secondary-care hospital in Rio de Janeiro, Brazil. In sputum, the IS6110-RFLP appeared in isolates with two variant patterns with 10 and 13 IS6110 copies. However, blood presented only the pattern corresponding to 10 copies, suggesting compartmentalization. With regard to the exact match of 10 of 13 bands, this may...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; TB-MDR; TB-RFLP; Gene mutation; Fingerprint.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500006

Fluorescent quantitative PCR of Mycobacterium tuberculosis for differentiating intestinal tuberculosis from Crohn's disease
Provedor de dados: 56
Autores: Fei,B.Y.; Lv,H.X.; Zheng,W.H.
Intestinal tuberculosis (ITB) and Crohn's disease (CD) are granulomatous disorders with similar clinical manifestations and pathological features that are often difficult to differentiate. This study evaluated the value of fluorescent quantitative polymerase chain reaction (FQ-PCR) for Mycobacterium tuberculosis (MTB) in fecal samples and biopsy specimens to differentiate ITB from CD. From June 2010 to March 2013, 86 consecutive patients (38 females and 48 males, median age 31.3 years) with provisional diagnoses of ITB and CD were recruited for the study. The patients' clinical, endoscopic, and histological features were monitored until the final definite diagnoses were made. DNA was extracted from 250 mg fecal samples and biopsy tissues from each patient....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Intestinal tuberculosis; Crohn's disease; Diagnosis; Fluorescent quantitative PCR; Mycobacterium tuberculosis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200166

Cordyceps sinensis promotes immune regulation and enhances bacteriostatic activity of PA-824 via IL-10 in Mycobacterium tuberculosis disease
Provedor de dados: 56
Autores: Li,D.G.; Ren,Z.X.
PA-824 is a novel bicyclic nitroimidazole anti-tuberculosis (TB) drug. Cordyceps sinensis (Berk.) Sacc. (CS) was proven to be a good immunomodulatory compound. This research aimed to investigate the effect of CS on PA-824 in Mycobacterium tuberculosis (M.tb) infected mice (female CBA/J mice, 6 to 8 weeks of age and 20±2 g of weight). Mice were randomly assigned to 4 groups: PA-824, CS, PA-824+CS, and control. To verify the effect of PA-824 and CS on M.tb, after drug administration, mice lungs were harvested and bacterial colony formations were measured. Cells were isolated from infected lungs and spleens to analyze the percentage of CD4+ T cells (CD11a positive). Lung cells were cultured to detect the secretion of interferon-γ (IFN-γ) and interleukin-10...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Cordyceps sinensis (Berk.) Sacc.; PA-824; IFN-γ; IL-10.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900604

Mycobacterium tuberculosis belonging to family LAM and sublineage RD Rio: common strains in Southern Brazil for over 10 years
Provedor de dados: 58
Autores: Soares,Renata Oliveira; Macedo,Maíra Bidart de; von Groll,Andrea; Silva,Pedro Eduardo Almeida da.
A sublineage of Mycobacterium tuberculosis called RD Rio was described in 2007. Although only recently described, this strain may have been present previously in the population, and its identification in clinical isolates will elucidate bacterial transmission dynamics and host-pathogen interactions. This study evaluated the clonal diversity of the RD Rio sublineage in clinical isolates from Rio Grande-RS obtained between 1998 and 2001. Among the 45 samples analyzed by the MIRU-VNTR method, there were six clusters with two samples each and 33 orphan strains with unique pattern. The strains were distributed across several different lineages including LAM (34.04%), X (14.89%), Haarlem (12.77%), UgandaI (10.64%), S (4.26%), NEW-1 (2.13%) and Cameroon (2.13%);...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; MIRU-VNTR; RDRio.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400032

Evaluation of a nested-pcr for Mycobacterium tuberculosis detection in blood and urine samples
Provedor de dados: 58
Autores: Cruz,Heidi Lacerda Alves da; Montenegro,Rosana de Albuquerque; Lima,Juliana Falcão de Araújo; Poroca,Diogo da Rocha; Lima,Juliana Figueirêdo da Costa; Montenegro,Lílian Maria Lapa; Crovella,Sergio; Schindler,Haiana Charifker.
The polymerase chain reaction (PCR) and its variations, such as the nested-PCR, have been described as promising techniques for rapid diagnosis of tuberculosis (TB). With the aim of evaluating the usefulness of a nested-PCR method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element IS6110 specific of M. tuberculosis genome. Nested-PCR method showed higher sensitivity in patients with extrapulmonary tuberculosis (47.8% and 52% in blood and urine) when compared to patients with the pulmonary form of the disease (sensitivity of 29% and 26.9% in blood and urine), regardless of the type of biological sample used. The nested-PCR is a rapid technique that, even if...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Nested-PCR; Blood; Urine; Molecular diagnosis; IS6110 insertion sequence.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100041

Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis
Provedor de dados: 58
Autores: Sales,Mariana L.; Fonseca Júnior,Antônio Augusto; Orzil,Lívia; Alencar,Andrea Padilha; Silva,Marcio Roberto; Issa,Marina Azevedo; Soares Filho,Paulo Martins; Lage,Andrey Pereira; Heinemann,Marcos Bryan.
Mycobacterium tuberculosis is the major cause of tuberculosis in humans. This bacillus gained prominence with the occurrence of HIV, presenting itself as an important opportunistic infection associated with acquired immunodeficiency syndrome (AIDS). The current study aimed to develop a real-time PCR using Eva Green technology for molecular identification of M. tuberculosis isolates. The primers were designed to Rv1510 gene. Ninety nine samples of M. tuberculosis and sixty samples of M. bovis were tested and no sample of the bovine bacillus was detected by the qPCR. Statistical tests showed no difference between the qPCR and biochemical tests used to identify the Mycobacterium tuberculosis. The correlation between tests was perfect with Kappa index of 1.0...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Diagnosis; Real time PCR.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400029

Do DNA extraction methods and Taq polimerase quality improve the double repetitive element (DRE) PCR typing method for Mycobacterium tuberculosis strains?
Provedor de dados: 58
Autores: Cavalcanti,Hebe Rodrigues; Marques,Elizabeth; Fonseca,Leila de Souza; Saad,Maria Helena Féres.
Double repetitive element (DRE) PCR amplification is a simple Mycobacterium tuberculosis typing method, however amplification failure or poor resolution of bands commit its efficacy. In order to verify if whether or not these features could be minimized by improving DNA extraction procedures or Taq polymerise quality, DRE-PCR was performed on 24 M. tuberculosis DNA samples extracted by heat-shock, mechanical and enzymatic methods applying conventional and hot start Taq pol. We demonstrated that when dealing with the Mycobacterium tuberculosis DRE-PCR typing method, Taq pol of better quality might be more important to improve amplification than the DNA extraction method.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; PCR; Taq hot start; DRE-PCR.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300004

Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
Provedor de dados: 58
Autores: Barouni,Abdulmonam Saied; Saridakis,Halha Ostrensky; Vidotto,Marilda Carlos.
We investigated the use of multiprimer-PCR for detection of mycobacteria species in clinical samples. Three different mycobacterial genomic fragments were investigated: the IS6110 insertion sequence, present in M. tuberculosis complex; the genus specific fragment (32kDa); and from M. tuberculosis species-specific mtp40 gene. The sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in Löwenstein-Jensen medium; the detection limit was 0.05ng of DNA. In conclusion, this assay is reliable and rapid for detection of Mycobacterium species in clinical samples, and differentiates M. tuberculosis from M. bovis strains in a single-step assay.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Multiprimer-PCR; Molecular diagnosis.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100004

Anti-Mycobacterium tuberculosis activity of fungus Phomopsis stipata
Provedor de dados: 58
Autores: Prince,Karina Andrade de; Sordi,Renata; Pavan,Fernando Rogério; Santos,Adolfo Carlos Barreto; Araujo,Angela R.; Leite,Sergio R.A.; Leite,Clarice Q. F.
Our purpose was to determine the anti-Mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus Phomopsis stipata (Lib.) B. Sutton, (Diaporthaceae), cultivated in different media. The antimycobacterial activity was assessed through the Resazurin Microtiter Assay (REMA) and the cytotoxicity test performed on macrophage cell line. The extracts derived from fungi grown on Corn Medium and Potato Dextrose Broth presented the smallest values of Minimum Inhibitory Concentration (MIC) and low cytotoxicity, which implies a high selectivity index. This is the first report on the chemical composition and antitubercular activity of metabolites of P. stipata, as well as the influence of culture medium on these properties.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phomopsis stipata; Antimycobacterial activity; Mycobacterium tuberculosis.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100024

Measuring of Mycobacterium tuberculosis growth: a correlation of the optical measurements with colony forming units
Provedor de dados: 58
Autores: Peñuelas-Urquides,Katia; Villarreal-Treviño,Licet; Silva-Ramírez,Beatriz; Rivadeneyra-Espinoza,Liliana; Said-Fernández,Salvador; León,Mario Bermúdez de.
The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bacterial growth; Colony forming units; McFarland; Mycobacterium tuberculosis; Optical density.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100042

Ethambutol resistance of indigenous Mycobacterium tuberculosis isolated from human patients
Provedor de dados: 58
Autores: Nazir,Taha; Rasool,Muhammad Hidayat; Hameed,Abdul; Ahmad,Bashir; Qureshi,Javed Anver.
The present study was conducted to find out the ethambutol resistance pattern of indigenous isolates of Mycobacterium tuberculosis from Tuberculosis diagnosed human patients. A total of 172 specimens were collected from six different sources and comprised of 84.9% sputum, 10.5% pus and 4.7% bronchial washings. There were 70.9% males and 29.1% females with 84.30% pulmonary and 15.69% extra-pulmonary tuberculosis. The Mycobacterium tuberculosis isolates collected from primary culture were further studied to determine their pattern and level of resistance. The inoculums were prepared using 0.5 Mac Farland turbidity standards. Five different concentration of ethambutol were used in Lowenstein Jensen (LJ) medium i.e. 2μg/ml, 4μg/ml, 6μg/ml, 8μg/ml and 10μg/ml...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethambutol; Mycobacterium tuberculosis; Resistance; Lowenstein Jensen medium; Human patients.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400026

Comparison of resazurin microtiter assay performance and BACTEC MGIT 960 in the susceptibility testing of Brazilian clinical isolates of Mycobacterium tuberculosis to four first-line drugs
Provedor de dados: 58
Autores: Miyata,Marcelo; Pavan,Fernando Rogério; Sato,Daisy Nakamura; Marino,Leonardo Biancolino; Hirata,Mario Hiroyuki; Cardoso,Rosilene Fressati; Melo,Fernando Augusto Fiúza de; Leite,Clarice Queico Fujimura.
We assessed the performance of REMA in comparison with BACTEC MGIT 960 in the susceptibility testing of 80 Mycobacterium tuberculosis clinical isolates from Clemente Ferreira Institute against four drugs. REMA proved to be a rapid and accurate method, providing excellent correlation with BACTEC MGIT 960, with the exception of results for the ethambutol drug.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Tuberculosis; Drug susceptibility testing.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100041

Microcolony detection in thin layer culture as an alternative method for rapid detection of Mycobacterium tuberculosis in clinical samples
Provedor de dados: 58
Autores: Silva,Pedro Eduardo Almeida da; Wiesel,Fernanda; Boffo,Maria Marta Santos; von Groll,Andréa; Mattos,Ivo Gomes de; Mejia,Glória; Robledo,Jaime.
Tuberculosis remains one of the major causes of mortality worldwide. The early detection of new cases is an important goal in the program of tuberculosis control. Several methodologies for rapid and accurate laboratorial diagnosis have been developed, however, some of these techniques are expensive or cumbersome, making their implementation in low-income regions unfeasible. In this study, the thin layer culture method was compared with conventional culture method and it was observed that it provides earlier results and a presumptive species identification, being adequate alternative method for rapid laboratory diagnosis.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Rapid diagnosis; Mycobacterium tuberculosis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300007

PCR in the diagnosis of cutaneous tuberculosis
Provedor de dados: 58
Autores: Ogusku,Mauricio Morishi; Sadahiro,Aya; Hirata,Mário Hiroyuki; Hirata,Rosário D. C.; Zaitz,Clarisse; Salem,Júlia Ignez.
Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated(with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH)biopsies tissues samples stored at -20ºC. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Cutaneous tuberculosis; Polymerase Chain Reaction (PCR); Laboratory diagnosis.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200015

Combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis
Provedor de dados: 58
Autores: Gkaravela,Lamprini; Papadimitriou-Olivgeris,Matthaios; Foka,Antigoni; Kolonitsiou,Fevronia; Spiliopoulou,Anastasia; Charokopos,Nikolaos; Voulgaridis,Apostolos; Tsiamita,Maria; Marangos,Markos; Anastassiou,Evangelos D.; Spiliopoulou,Iris.
ABSTRACT Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus). PCR in the clinical specimens showed excellent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; PCR; GenoType MTBDRplus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400785

Improved diagnosis of central nervous system tuberculosis by MPB64-Target PCR
Provedor de dados: 58
Autores: Dil-Afroze,; Mir,Abdul Waheed; Kirmani,Altaf; Shakeel-ul-Rehman,; Eachkoti,Rafiqa; Siddiqi,Mushtaq A.
Central nervous system (CNS) tuberculosis is a serious clinical problem, the treatment of which is sometimes hampered by delayed diagnosis. Clearly, prompt laboratory diagnosis is of vital importance as the spectrum of disease is wideand abnormalities of the cerebrospinal fluid (CSF) are incredibly variable. Since delayed hypersensitivity is the underlying immune response, bacterial load is very low. The conventional bacteriological methods rarely detect Mycobacterium tuberculosis in CSF and are of limited use in diagnosis of tuberculous meningitis (TBM). This double blind study was, therefore, directed to the molecular analysis of CNS tuberculosis by an in-house-developed PCR targeted for amplification of a 240bp nucleotidesequence coding for MPB64...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CNS tuberculosis; Tuberculous meningitis (TBM); Intracranial tuberculomas; PCR; Mycobacterium tuberculosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200002

Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies
Provedor de dados: 58
Autores: von Groll,Andrea; Martin,Anandi; Portaels,Françoise; Silva,Pedro Eduardo Almeida da; Palomino,Juan Carlos.
We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth kinetics; Mycobacterium tuberculosis; Resazurin; Fitness.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200006

Cord formation and colony morphology for the presumptive identification of Mycobacterium tuberculosis complex
Provedor de dados: 58
Autores: Monteiro,Paulo Henrique Tasso; Martins,Maria Conceição; Ueki,Suely Yoko Mizuka; Giampaglia,Carmen Maria Saraiva; Telles,Maria Alice da Silva.
The identification of Mycobacterium tuberculosis complex (MT), using non-molecular methods, is time-consuming. The objective of this study was to evaluate a screening test for the presumptive identification of MT, which could potentially decrease laboratory turn-around time for reporting preliminary results. From January 1998 to December 1999, 3056 cultures were analysed at the Mycobacterial Laboratory, Instituto Adolfo Lutz, São Paulo, Brasil. The screening test consisted of observation of colony morphology on Löwenstein Jensen medium and evaluation of cord formation on smear microscopy from those positive cultures. After the screening test, the cultures identified as non-tuberculous mycobacteria were identified to species by conventional methods (growth...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; Identification; Cord formation; Culture.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200016

Comparative evaluation of the Nitrate Reductase Assay and the Resazurin Microtitre Assay for drug susceptibility testing of Mycobacterium tuberculosis against first line anti-tuberculosis drugs
Provedor de dados: 58
Autores: Sanchotene,Karine O.; von Groll,Andrea; Ramos,Daniela; Scholante,Ana B.; Honscha,Gunther; Valença,Mariana; Scaini,Carlos J.; Silva,Pedro E.A. da.
Tuberculosis remains as a serious infection disease of worldwide distribution, with high morbidity and mortality, mainly in low socio-economic condition countries. The state of emergency of tuberculosis caused by the resistant and multidrug-resistant (MDR) strains, became the main threat to the tuberculosis treatment and control programs. A fast detection method for the resistant strains will allow the implementation of an adequate treatment and contribute for controlling the dissemination of these resistant strains. This study evaluated the performance of the nitrate reductase assay in solid (NRA-LJ) and liquid (NRA-7H9) media, to determine the susceptibility to first line anti-tuberculosis drugs: isoniazid (INH), rifampicin (RMP), ethambutol (EMB) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Mycobacterium tuberculosis; Nitrate reductase assay; Susceptibility tests; Drug resistance.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100004

Bacteriological features of infectious spondylodiscitis at Mohammed V Military Teaching Hospital of Rabat
Provedor de dados: 58
Autores: Zohoun,A; Ngoh Akwa,E; El Ochi,M; Oragwu,N; Akhaddar,A; Albouzidi,A; El Ouennass,M.
To review the bacteriological features of infectious spondylodiscitis and provide recommendations for the initial therapy which remains empirical in our context. Retrospective study including patients diagnosed with spondylodiscitis over a period of 4 years (2006-2009) at the Rabat Military Teaching Hospital. During the study period, we analysed 30 cases: the mean age was 49.9 years and 21 cases (70%) were male. The patients were predominantly hospitalized in neurosurgery department (15/30) followed by rheumatology department (10/30). The site of infection was lumbar in 21 cases (21/30), dorsal in 7 cases (7/30). 26 cultures were positive of which 19 (19/26) were monomicrobial. Tuberculosis (TB) was implicated in 10 cases (10/30) including 4 cases in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Spondylodiscitis; Mycobacterium tuberculosis; Staphylococcus aureus; Empirical treatment; Morocco.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400013

Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
Provedor de dados: 58
Autores: Miyata,Marcelo; Santos,Adolfo Carlos Barreto; Mendes,Natália Helena; Cunha,Eunice Atsuko; Melo,Fernando Augusto Fiúza de; Leite,Clarice Queico Fujimura.
We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA extraction; Mycobacterium tuberculosis; Thermolysis; CTAB.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045

Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil
Provedor de dados: 58
Autores: Augusto,Cláudio José; Carvalho,Wânia da Silva; Almeida,Isabela Neves de; Figueiredo,Lida Jouca de Assis; Dantas,Nayanne Gama Teixeira; Suffys,Philip Noel; Miranda,Silvana Spíndola de.
Abstract DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat - Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) techniques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and 50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal to or above 85%. The RFLP-IS6110 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycobacterium tuberculosis; RFLP; VNTR loci.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300641

Antimicrobial activity of essential oils from Lippia alba, Lippia sidoides, Cymbopogon citrates, Plectranthus amboinicus, and Cinnamomum zeylanicum against Mycobacterium tuberculosis
Provedor de dados: 65
Autores: Mota,Aquiles Paulino Peres; Dantas,João Carlos Pinheiro; Frota,Cristiane Cunha.
ABSTRACT: The rise in cases of antibiotic-resistant Mycobacterium tuberculosis has become a major obstacle to the effective control of tuberculosis (TB) worldwide. Essential oils (EO) are complex mixtures that may contain between 20 and 60 components, with two or three major compounds at relatively high concentrations (20-70%) that are responsible for their pharmacological properties. The objective of this study was to assess the antimicrobial activity of the EOs, bushy lippia (Lippia alba), rosemary pepper (Lippia sidoides), lemon grass (Cymbopogon citratus), Mexican mint or Indian borage (Plectranthus amboinicus), and true cinnamon (Cinnamomum zeylanicum), against Mycobacterium tuberculosis H37Rv. Chemical characterization of the EOs was performed by gas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-bacterial agent; Essential oils; Mycobacterium tuberculosis; Lippia alba; Lippia sidoides; Cymbopogon citrates; Plectranthus amboinicus; Cinnamomum zeylanicum.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600453