Consulta: subjectFacets:"Mycotoxin"
Registros recuperados: 18
Data/hora: 06/06/2024 06:28:56

MEIS3 is repressed in A549 lung epithelial cells by deoxynivalenol and the repression contributes to the deleterious effect
Provedor de dados: 19
Autores: Toyotome, Takahito; Takahashi, Hiroki; Kamei, Katsuhiko.
Deoxynivalenol (DON) is an important Fusarium toxin of concern for food safety. The inhalation of powder contaminated with DON is possible and may cause lung toxicity. In this study, we analyzed the gene expression profile of A549 cells treated for 24 hr with 0.2 µg/mL DON by microarray analysis. In total, 16 genes and 5 noncoding RNAs were significantly affected by DON treatment. The repression of B3GALT4, MEIS3, AK7, SEMA3A, KCNMB4, and SCARA5 was confirmed by quantitative PCR. We investigated the DON toxicity on A549 cells that exogenously expressed these 6 genes. The result indicated that A549 cells that transiently expressed MEIS3 were tolerant to the deleterious effects of DON. Our data show that DON affected the expression of genes with various...
Palavras-chave: Fusarium toxin; Mycotoxin; Deoxynivalenol; Trichothecene; Lung epithelial cell.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4301

Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed
Provedor de dados: 86
Autores: Vaccari,Isabel Cristina Mores; Duarte,Vinicius; Rodrigues,Denise Russi; Santos,Fabiana Ramos dos; Minafra,Cibele Silva.
Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin; Aviculture; Fumonisin; Mycotoxin; T-2 toxin; Vomitoxin.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000942

Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain
Provedor de dados: 86
Autores: Silva,André Ribeiro da; Zanin,Lívia Montanheiro Médici; Ishikawa,Angélica Tieme; Yamashita,Cassia Reika Takabayashi; Fracalossi,Felipe Pedote; Amorim,Thaís Marques; Itano,Eiko Nakagawa; Kawamaura,Osamu; Hirooka,Elisa Yoko.
Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Aflatoxin B1; Blanched peanuts; Contamination; Mycotoxin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300361

Effects of fusaric acid on Zea mays L. seedlings
Provedor de dados: 85
Autores: Diniz,SPSS; Oliveira,RC.
The effects of fusaric acid, a mycotoxin produced by Fusarium sp, were investigated in relation to its action on corn seedlings. The mycotoxin inhibited the development of corn seedlings at 0.2 mM, causing root length reduction. Anatomically, alterations were only visible from 0.5 mM fusaric acid, directly influencing the cell differentiation process. Precocious differentiation reduces the elongation region. This explains (1) that root shortening is morphologically visible, and (2) the differentiation of a great number of lateral roots nearby the apex, which can be seen in longitudinal cuts.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fusaric acid; Mycotoxin; Zea mays seedlings.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200012

Effects of ochratoxin a on broiler leukocytes
Provedor de dados: 89
Autores: Moura,MA; Machado,CH; Porfírio,LC; Freire,RB.
This study evaluated alterations in the qualitative cellular profile of leukocytes caused by the administration of low doses of ochratoxin-A (OTA) in poultry. Sixty chicks were separated in three experimental groups: control, PBS-treated and OTA-treated. Blood smears from all birds were analyzed three and six hours post-treatment. Differential leukocyte counting demonstrated that OTA reduced the percentage of lymphocytes and eosinophils and significantly increased the number of heterophils and monocytes.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Hematology; Mycotoxin; Ochratoxin A.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000300010

Molecular identification of Aspergillus spp. isolated from coffee beans
Provedor de dados: 63
Autores: Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli.
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009

Mycotoxin in mold corn, detoxification and utilization of mold corn in chick rations
Provedor de dados: 106
Autores: Prateap Rachapaetayakom; Ankana Hanbranchong; Suporn Sukhupanyaraxa.
4 tables
Palavras-chave: Corn; Maize; Chickens; Mycotoxin; Mold corn; Detoxification; Rations; Ingredients; Feeds; Growth; ข้าวโพด; สารพิษจากเชื้อรา; การทำลายสารพิษ; สูตรอาหาร; ลูกไก่.
Ano: 1977
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3943

Study of grain mold inoculation and screening technique by using Mycotoxin
Provedor de dados: 106
Autores: Tuanchai Boon-long; Treelote Sukhonthachaya; Niyom Chew-Chin.
Palavras-chave: Sorghum; Grain mold; Curvularia; Fusarium; Colletotrichum; Phoma; Inoculation; Screening technique; Mycotoxin; ข้าวฟ่าง; โรคราบนช่อข้าวฟ่าง; เชื้อสาเหตุ.
Ano: 1982
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4101

Animal nutrition studies
Provedor de dados: 106
Autores: Prateap Rachapaetayakom.
20 tables
Palavras-chave: Chickens; Chicks; Corn; Maize; Opaque-2; Protein diet; Rations; Nutritive value; Varieties; Mycotoxin; Feed contamination; Growth; Animal performance; ข้าวโพด; ยีนโอเพค 2; โปรตีน; สูตรอาหาร; ลูกไก่; ไก่กระทง.
Ano: 1971
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3767

Effects of fed mycotoxin contaminated diets supplemented with spray-dried porcine plasma on cholinergic response and behavior in piglets
Provedor de dados: 42
Autores: MÜLLER,LUCIELI K.F.; SILVA,ALEKSANDRO S. DA; BOTTARI,NATHIELI B.; SANTURIO,JANIO M.; MORSCH,VERA M.; PIVA,MANOELA M.; MENDES,RICARDO E.; GLORIA,EDUARDO M.; PAIANO,DIOVANI.
Abstract: The aim of this study was to evaluate the effect of spray-dried porcine plasma (SDPP) supplementation on cholinesterase enzymes and its relationship with animal behavior of weaning piglets exposed to mycotoxin contaminated diets. To achieve these objectives, two experimental design approaches were used. Male piglets (7.15±0.61kg) were allocated in four groups: CTL group received a regular diet; SDPP group received a regular diet and 6% SDPP; MYC group received a diet containing desired contamination of 210 µg/kg aflatoxins and 6.690 µg/kg fumonisins; group MYC+SDPP received 253 µg/kg aflatoxins, 6930 µg/kg fumonisins and 6% SDPP. The animals treated with mycotoxin co-contaminated diets showed an increase in AChE and BChE activities in peripheral...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycotoxin; Acetylcholinesterase; Brain; Spray-dried porcine plasma; Animal health.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300807

Occurrence of killer yeast strains in industrial and clinical yeast isolates
Provedor de dados: 48
Autores: BAEZA,MARCELO E; SANHUEZA,MARIO A; CIFUENTES,VÍCTOR H.
The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsRNA) or in nuclear genes. The spectrum of action and the activity of killer toxins are influenced by temperature, salinity and pH of media. In the present work we determined the existence of K+ in a collection of S. cerevisiae and P. anómala yeasts isolated from environmental, industrial and clinical sources. The assays were performed in strains belonging to three yeast genera used as sensitive cells and under a wide range of pH and temperatures. Approximately 51 % of...
Tipo: Journal article
Palavras-chave: Killer system; Mycotoxin; DsRNA.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200007

Identification of a splicing coactivator gene that affects the production of ochratoxin a in Aspergillus carbonarius
Provedor de dados: 52
Autores: Lunardi,Lígia Uno; Guembarovski,Roberta Losi; Hanai,Luiz Ricardo; Cristiano,Valderi; Vieira,Maria Lucia Carneiro; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli.
Ochratoxin A is a mycotoxin produced by some fungi species. Among them, Aspergillus carbonarius is considered a powerful producer. Genes involved in the ochratoxin A biosynthesis pathway have been identified in some producer species. However, there are few studies that purpose to identify these genes in A. carbonarius. The use of insertion mutants to identify genes associated with certain properties has been increased in the literature. In this work, the region of T-DNA integration was investigated in one A. carbonarius ochratoxin-defective mutant previously obtained by Agrobacterium tumefaciens-mediated transformation, in order to find an association between interrupted gene and the biosynthesis of ochratoxin A. The integration occurred in a gene that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxigenic fungi; Mycotoxin; Real time PCR; Splicing coactivator protein.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700018

Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil
Provedor de dados: 52
Autores: Trombete,Felipe Machado; Castro,Izabela Miranda de; Teixeira,Alessandra da Silva; Saldanha,Tatiana; Fraga,Marcelo Elias.
This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycotoxin; Dairy products; HPLC-FLD.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016

DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay
Provedor de dados: 56
Autores: Zeljezic,D.; Domijan,A.-M.; Peraica,M.
There are few studies of ochratoxin A (OTA) genotoxicity in experimental animals and the results obtained with cell cultures are inconsistent, although the carcinogenic potential of OTA for the kidney of experimental animals has been well established. We studied the genotoxic potential of OTA in the kidney of adult female Wistar rats (5 in each group) treated intraperitoneally with OTA (0.5 mg kg body weight-1 day-1 for 7, 14, and 21 days) measuring DNA mobility on agarose gel stained with ethidium-bromide using standard alkaline single-cell gel electrophoresis (comet assay). Negative control animals were treated with solvent (Tris buffer, 1.0 mg/kg) and positive control animals were treated with methyl methanesulfonate (40 mg/kg) according to the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Comet assay; DNA adducts; Experimental animals; Mycotoxin.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200006

Occurrence of aflatoxin B1 in natural products
Provedor de dados: 58
Autores: Prado,Guilherme; Altoé,Aline F.; Gomes,Tatiana C. B.; Leal,Alexandre S.; Morais,Vanessa A. D.; Oliveira,Marize S.; Ferreira,Marli B.; Gomes,Mateus B.; Paschoal,Fabiano N.; Souza,Rafael von S.; Silva,Daniela A.; Madeira,Jovita E. G. Cruz.
The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Medicinal plants; Fungi; Mycotoxin; High-performance liquid chromatography; HPLC.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026

Effect of fungicide on Fusarium verticillioidesmycelial morphology and fumonisin B1 production
Provedor de dados: 58
Autores: Miguel,Tatiana de Á.; Bordini,Jaqueline G.; Saito,Gervásio H.; Andrade,Célia G.T. de J.; Ono,Mario A.; Hirooka,Elisa Y.; Vizoni,Édio; Ono,Elisabete Y.S.
The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that the fungicide caused inhibition of hyphal growth and defects on hyphae morphology such as cell wall disruption, withered hyphae, and excessive septation. In addition, extracellular material around the hyphae was rarely observed in the presence of fludioxonil + metalaxyl-M. While promoting the reduction of mycelial growth, the fungicide increased sporulation of F. verticillioides compared to the control, and the highest production occurred on the 14th day in the treatments and on the 10th day in the control cultures. Fumonisin B1production in the culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxigenic fungi; Mycotoxin; Scanning electron microscopy; Electron micrographs; Extracellular material.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100293

Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC)
Provedor de dados: 117
Autores: Moyano A,Stella; Lanuza,Francisco; Torres B,Alfredo; Cisternas A,Ernesto; Fuentes V,Marcela.
Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pasture in southern Chile, it is necessary to know the content of lolitrem-B to optimize grazing management and reduce the effect on animal health. The objective of this study was to implement a method to determine lolitrem-B in ryegrass. Ryegrass samples were used without the endophyte, freeze-dried, and kept in darkness at -18 ºC to establish the method. Then, a pure lolitrem-B standard was added and the toxin was extracted with a mixture of chloroform-methanol...
Tipo: Journal article
Palavras-chave: Mycotoxin; Neurotoxin; Endophyte fungi.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019

Mycological quality of pecan nuts from Brazil: absence of aflatoxigenic fungi and aflatoxins
Provedor de dados: 65
Autores: Garcia,Marcelo Valle; Moraes,Vivian Machado; Bernardi,Angélica Olivier; Oliveira,Maurício Schneider; Mallmann,Carlos Augusto; Boscardin,Jardel; Copetti,Marina Venturini.
ABSTRACT:  In recent years, Brazil has encouraged the cultivation of pecans to meet both demands of domestic and international market of nuts. New genetic varieties of pecans have been selected in recent years, but available scientific information on the occurrence of fungi and aflatoxins in the international literature is out of date. Therefore, the present study aimed to quantify and identify fungal microbiota and the presence of aflatoxins in pecan nuts cultivated in southern Brazil. Fifty-two pecan nut lots (Barton variety) were obtained from producers from 19 cities of Rio Grande do Sul State and analyzed by direct plating in Agar Dichloran Glycerol 18% (DG18) and Aspergillus Flavus and Parasiticus Agar (AFPA), following incubation at 25 °C for 7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Penicillium; Mycotoxin; Rio Grande do Sul; Carya illinoinensis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600752