Consulta: subjectFacets:"Nanoparticles"
Registros recuperados: 31
Data/hora: 05/06/2024 18:47:38

Diseño y evaluación de nanoparticulas de selenito de sodio para su uso en rumiantes
Provedor de dados: 32
Autores: Romero Pérez, Atmir.
El selenito de sodio, el suplemento de selenio mas usado en rumiantes en la  prevención de la deficiencia del elemento y las enfermedades resultantes, fue  encapsulado por nanoprecipitación y emulsión-evaporación en nanopartículas  poliméricas. Fue evaluado el efecto del método de preparación de nanoparticulas,  proporciones de polímeros (Eudragit RL 100 y RS 100) y solvente empleado  (etanol y acetona), sobre las características fisicoquímicas (potencial z, índice de  polidispersidad y porcentaje de encapsulación) y la morfología (tamaño de  partícula y forma) de las nanopartículas. El tamaño de partícula obtenido presentó  valores de 34.64 a 213.86 nm. Tanto el tamaño como el potencial z y el PDI se  incrementaron (P &lt; 0.05) cuando se utilizó el...
Tipo: Tesis
Palavras-chave: Nanopartículas; Selenito de sodio; Eudragit; Rumiantes; Maestría; Ganadería; Nanoparticles; Sodium selenite; Eudragit; Ruminants.
Ano: 2008
URL: http://hdl.handle.net/10521/1515

Preparation and characterization of &amp;#954;-carrageenase immobilized onto magnetic iron oxide nanoparticles
Provedor de dados: 69
Autores: Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong.
Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and &amp;#954;-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized &amp;#954;-carrageenase were investigated. Results The &amp;#954;-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for &amp;#954;-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U &amp;#954;-carrageenase for 20 mg of magnetic nanoparticles, a 2-h...
Tipo: Journal article
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001

Viabilidade técnica e econômica de processo simulado para produção de celulose bacteriana em melaço de soja.
Provedor de dados: 14
Autores: SILVA NETO, R. M. da; MAIA, C. W. C. P.; ROSA, M. de F.
A celulose é uma das principais moléculas naturais utilizadas e tem sido tradicionalmente usada para fabricação de papel, além de outras aplicações. Algumas bactérias, em especial as do gênero Komagataeibacter, são capazes de produzir um biopolímero com estrutura química similar à celulose vegetal, denominado celulose bacteriana (CB). Dessa forma, podem, em determinadas aplicações, ser uma fonte alternativa à celulose vegetal, a qual é mundialmente utilizada pelas indústrias de um modo geral. A celulose vegetal e a CB apresentam a mesma estrutura molecular, mas a segunda tem vantagens competitivas, incluindo uma estrutura em rede naturalmente nanométrica, que atraíram atenção para sua produção. Em comparação com a celulose vegetal, a CB apresenta...
Tipo: Documentos (INFOTECA-E)
Palavras-chave: Celulose bacteriana; Nanopartículas; Melaço de soja; Soy molasses; Bacterial cellulose; Nanoparticles.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1130078

New SERS Substrates For Polycyclic Aromatic Hydrocarbon (PAH) Detection: Towards Quantitative SERS Sensors For Environmental Analysis
Provedor de dados: 5
Autores: Peron, Olivier; Rinnert, Emmanuel; Compere, Chantal; Toury, T.; De La Chapelle, M. Lamy.
In the investigation of chemical pollutions, such as PAHs (Polycyclic Aromatic Hydrocarbons) at low concentration in aqueous medium, surface-enhanced Raman scattering (SERS) stands for an alternative to the inherent low cross-section of normal Raman scattering. Indeed, SERS is a very sensitive spectroscopic technique due to the excitation of the surface plasmon modes of the nanostructured metallic film.
Tipo: Text
Palavras-chave: Quantitative SERS sensors; Polystyrene beads; PAHs; Nanoparticles.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/00012/12328/9113.pdf

Ultrastructural Analyses of Platelets and Fibrin Networks in BALB/c Mice after Inhalation of Spherical and Rod-Shaped Titanium Nanoparticles
Provedor de dados: 77
Autores: Oosthuizen,Maria Aletta; Pretorius,Etheresia; Oberholzer,Hester Magdalena; van der Spuy,Wendy Jeannette.
Engineered nanoparticles are designed to perform specific functions and therefore have specific properties that could potentially be harmful. Nanoparticles such as titanium dioxide have the potential to become transparent and are therefore widely used in cosmetic products and sunscreen. Research on the toxicity of nanoparticles is of utmost importance and numerous in vitro studies have shown that some of these particles could have adverse health effects. The current study aimed to investigate the in vivo effects of two different titanium nanoparticles at two different concentrations after inhalation by experimental BALB/c mice. This was done to determine whether these particles will cause an inflammatory reaction, visible as alterations in platelet and...
Tipo: Journal article
Palavras-chave: Nanoparticles; TiO2; Platelets; Fibrin; BALB/c murine model.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400044

Nanoparticulate drug delivery systems for the treatment of neglected tropical protozoan diseases
Provedor de dados: 80
Autores: Volpedo,Greta; Costa,Lourena; Ryan,Nathan; Halsey,Gregory; Satoskar,Abhay; Oghumu,Steve.
Abstract Neglected Tropical Diseases (NTDs) comprise of a group of seventeen infectious conditions endemic in many developing countries. Among these diseases are three of protozoan origin, namely leishmaniasis, Chagas disease, and African trypanosomiasis, caused by the parasites Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei respectively. These diseases have their own unique challenges which are associated with the development of effective prevention and treatment methods. Collectively, these parasitic diseases cause more deaths worldwide than all other NTDs combined. Moreover, many current therapies for these diseases are limited in their efficacy, possessing harmful or potentially fatal side effects at therapeutic doses. It is therefore...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmaniasis; Chagas; Trypanosomiasis; Nanoparticles; Liposomes; Nanotubes; Nanodiscs; Micelles.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100201

Jaburetox: update on a urease-derived peptide
Provedor de dados: 80
Autores: Becker-Ritt,Arlete Beatriz; Portugal,Camila Saretta; Carlini,Célia Regina.
Abstract Urease from Canavalia ensiformis seeds was the first enzyme ever to be crystallized, in 1926. These proteins, found in plants, bacteria and fungi, present different biological properties including catalytic hydrolysis of urea, and also enzyme-independent activities, such as induction of exocytosis, pro-inflammatory effects, neurotoxicity, antifungal and insecticidal properties. Urease is toxic to insects and fungi per se but part of this toxicity relies on an internal peptide (~11 kDa), which is released upon digestion of the protein by insect enzymes. A recombinant form of this peptide, called jaburetox (JBTX), was constructed using jburell gene as a template. The peptide exhibits liposome disruption properties, and insecticidal and fungicidal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peptide; Bacteria; Membranes; Nanoparticles.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100211

Accumulation of silver nanoparticles and its effect on the antioxidant capacity in Allium cepa L
Provedor de dados: 85
Autores: Juárez-Maldonado,A; Rosales-Velázquez,JL; Ortega-Ortiz,H; Cabrera-De-la-Fuente,M; Ramírez,H; Benavides-Mendoza,A.
Nanotechnology is currently an important worldwide study field because it provides control on matter at a nanometric scale. In food and agricultural fields, the applications of this technology are in early stages. Onion is one of the main vegetables grown in the world. Onion is a low calorie food which contains a large amount of flavonoids. In this work, silver nitrate application in onion was evaluated. An onion crop was developed at the University Antonio Narro in Saltillo, Mexico. Two methods of silver nitrate application were used (a nutritive solution: 0, 20, 40 and 80 mg/L of AgNO3; and foliar spray: 0, 20 and 40 mg/L of AgNO3). The samples were taken at 30, 60 and 90 days after transplanting. Total antioxidant capacity, silver accumulation and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanotechnology; Onion; Silver nitrate; Nanoparticles.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572013000100013

Use of Nanoparticles in Soil-Water Bioremediation Processes
Provedor de dados: 127
Autores: Duran,N.
Tipo: Journal article
Palavras-chave: Nanoparticles; Bioremediation processes; Nanotechnology.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000400007

TiO2 nanoparticles as an effective nanopesticide for cotton leaf worm
Provedor de dados: 111
Autores: shaker, ahmed mahmoud.
The Egyptian cotton leaf worm, Spodoptera littoralis (Boisd.) (Noctuidae: Lepidoptera) is an economically important pest with a wide range of host plants. It attacks certain vegetable and field crops such as cotton, tomatoes, cabbage and squash in Egypt, for causing severe injuries to the plants in all phonological crop stages, In addition to this species has acquired resistance to many insecticides. The overall objective of this investigations was to look for new control strategy through evaluate the effects of application of TiO2 nanoparticles on the survival and biology of this insect. The experiment were carried out under laboratory conditions, the experiment was achieved with two treatments, consisting of TiO2 nanoparticles application, and a negative...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: TiO2; Nanoparticles; Cotton; Leaf worm; Nanopesticide; Agriculture.
Ano: 2018
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4390

Characterization and in vitro antitumor activity of polymeric nanoparticles loaded with Uncaria tomentosa extract
Provedor de dados: 42
Autores: RIBEIRO,ANA F.; SANTOS,JULIANA F.; MATTOS,RÔMULO R.; BARROS,ELIANE G.O.; NASCIUTTI,LUIZ EURICO; CABRAL,LÚCIO M.; SOUSA,VALERIA P. DE.
Abstract  Uncaria tomentosa (UT) extracts have been shown to have promising anti-tumor activity. We hypothesized that its incorporation into nanostructured systems could improve the anticancer properties. Here, poly-e-caprolactone (PCL) and poly-d,l-lactide-co-glycolide (PLGA) were employed to generate nanoparticles loaded with UT extract in a single emulsion solvent evaporation method. The nanoparticles were characterized by particle size, zeta potential, morphology and entrapment efficiency along with stability and release profiles. The nanoparticles presented entrapment efficiencies above 60% and a mean diameter below 300nm. UT-PCL nanoparticles presented higher entrapment efficiency and mean particle size as well as a slow release rate. The UT-PLGA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Prostate cancer; PLGA; PCL; Anti-tumor; Anti-cancer; Cat’s Claw.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100908

Size and shape-controlled nanomaterials based on modified polyol and thermal decomposition approaches. A brief review.
Provedor de dados: 42
Autores: VARANDA,LAUDEMIR C.; SOUZA,CAIO G.S.; MORAES,DANIEL A.; NEVES,HERBERT R.; SOUZA JUNIOR,JOÃO B.; SILVA,MONICA F.; BINI,RAFAEL A.; ALBERS,REBECCA F.; SILVA,TIAGO L.; BECK JUNIOR,WATSON.
Abstract: Since its inception in 2007, the Colloidal Materials Group, one of the research groups in nanotechnology of the Institute of Chemistry of São Carlos (IQSC) at University of São Paulo (USP) carries out studies related to the development of syntheses methods of nanoparticles and multifunctional nanostructured systems. Our works search new synthesis methodologies that allow high size and shape control of the individual nanoparticles or that compose the nanostructured systems and has as a principle the synthetic approaches based on the modified polyol and thermal decomposition processes. Systems involving nanoparticles have received extensive attention both in the fundamental research and in several technological applications exploring the unique...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Nanostructured composites; Modified polyol process; Thermal decomposition method; Size and shape control; Colloidal Materials Group.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700406

Amyloid-β peptide absence in short term effects on kinase activity of energy metabolism in mice hippocampus and cerebral cortex
Provedor de dados: 42
Autores: IANISKI,FRANCINE R.; RECH,VIRGINIA C.; NISHIHIRA,VIVIAN S.K.; ALVES,CATIANE B.; BALDISSERA,MATHEUS D.; WILHELM,ETHEL A.; LUCHESE,CRISTIANE.
ABSTRACT Considering that Alzheimer's disease is a prevalent neurodegenerative disease worldwide, we investigated the activities of three key kinases: creatine kinase, pyruvate kinase and adenylate kinase in the hippocampus and cerebral cortex in Alzheimer's disease model. Male adult Swiss mice received amyloid-β or saline. One day after, mice were treated with blank nanocapsules (17 ml/kg) or meloxicam-loaded nanocapsules (5 mg/kg) or free meloxicam (5 mg/kg). Treatments were performed on alternating days, until the end of the experimental protocol. In the fourteenth day, kinases activities were performed. Amyloid-β did not change the kinases activity in the hippocampus and cerebral cortex of mice. However, free meloxicam decrease the creatine kinase...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Alzheimer's disease; Nanoparticles; Meloxicam; Energetic metabolism; Phosphoryltransfer network.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501829

Perspectives of Dendrimer-based Nanoparticles in Cancer Therapy
Provedor de dados: 42
Autores: CASTRO,RICARDO I.; FORERO-DORIA,OSCAR; GUZMÁN,LUIS.
Abstract Currently, cancer is the second most common cause of death in the United States, exceeded only by heart disease. Chemotherapy traditionally suffers from a non-specific distribution, with only a small fraction of the drug reaching the tumor, in this sense, the use of dendrimers incorporating drugs non-covalently encapsulated inside the dendrimer or covalently conjugated have proven to be effectives against different cancer cell lines. However, at present the dendrimers used as drug-carriers still do not meet the necessary characteristic to be considered as an ideal dendrimer for drug delivery; high toxicity, bio-degradability, low toxicity, biodistribution characteristics, and favorable retention with appropriate specificity and bioavailability...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimers; Cancer therapy; Pharmacodynamics; Nanoparticles.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502331

Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
Provedor de dados: 48
Autores: SÁNCHEZ,GITTITH; CUELLAR,DANIELA; ZULANTAY,INES; GAJARDO,MARTA; GONZÁLEZ-MARTIN,GUILLERMO.
The aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate replicative intracellular form is amastigote, in vitro studies were performed on this form of parasite as well as on cell culture derived trypomastigotes. The fluorescence method used here was very useful as it allowed for the simultaneous study of trypanocide activity and cytotoxicity by determining living or dead parasites within living or dead host cells. According to these results, the greatest trypanocide activity on cell culture-derived trypomastigotes was recorded...
Tipo: Journal article
Palavras-chave: Nifurtimox; Nanoparticles; Amastigotes; Trypomastigotes; Trypanosoma cruzi.
Ano: 2002
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100007

Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines
Provedor de dados: 48
Autores: Rivas-Aravena,Andrea; Sandino,Ana María; Spencer,Eugenio.
Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fish from bacterial, viral and parasitic diseases. However, the production of effective vaccines is limited by the scarcity of knowledge about the immune system of fish. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more effective and less harmful to the fish. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of different types to apply in fish vaccines. This work reviews the new methods to improve fish vaccines by encapsulating them in polymers and...
Tipo: Journal article
Palavras-chave: Nanoparticles; Vaccine; Aquaculture.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400012

Investigation of Structural, Morphological, Magnetic Properties and Biomedical applications of Cu2+ Substituted Uncoated Cobalt Ferrite Nanoparticles
Provedor de dados: 52
Autores: Margabandhu,M.; Sendhilnathan,S.; Senthilkumar,S.; Gajalakshmi,D.
ABSTRACT In the present work, Cu2+ substituted cobalt ferrite (Co1-xCuxFe2O4, x = 0, 0.3, 0.5, 0.7 and 1) magnetic nanopowders were synthesized via chemical co-precipitation method. The prepared powders were investigated by various characterization methods such as X-ray diffraction analysis (XRD), scanning electron microscope analysis (SEM), vibrating sample magnetometer analysis (VSM) and fourier transform infrared spectroscopy analysis (FTIR). The XRD analysis reveals that the synthesized nanopowders possess single phase centred cubic spinel structure. The average crystallite size of the particles ranging from 27-49 nm was calculated by using Debye-scherrer formula. Magnetic properties of the synthesized magnetic nanoparticles are studied by using VSM....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Co-precipitation; Cubic spinel; Morphology; Anti-bacterial activity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000300401

Evaluation of antibacterial properties of Barium Zirconate Titanate (BZT) nanoparticle
Provedor de dados: 58
Autores: Mohseni,Simin; Aghayan,Mahdi; Ghorani-Azam,Adel; Behdani,Mohammad; Asoodeh,Ahmad.
So far, the antibacterial activity of some organic and inorganic compounds has been studied. Barium zirconate titanate [Ba(Zr xTi1-x)O3] (x = 0.05) nanoparticle is an example of inorganic materials. In vitro studies have provided evidence for the antibacterial activity of this nanoparticle. In the current study, the nano-powder was synthesized by sol-gel method. X-ray diffraction showed that the powder was single-phase and had a perovskite structure at the calcination temperature of 1000 ºC. Antibacterial activity of the desired nanoparticle was assessed on two gram-positive (Staphylococcus aureus PTCC1431 and Micrococcus luteus PTCC1625) and two gram-negative (Escherichia coli HP101BA 7601c and clinically isolated Klebsiella pneumoniae) bacteria according...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Antibiotics; Barium zirconate titanate; Ceramics; Electron microscopy.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400033

Glycodendrimers: versatile tools for nanotechnology
Provedor de dados: 60
Autores: Roy,René; Shiao,Tze Chieh; Rittenhouse-Olson,Kate.
Combining nanotechnology with glycobiology has triggered an exponential growth of research activities in the design of novel functional bionanomaterials (glyconanotechnology). More specifically, recent synthetic advances towards the tailored and versatile design of glycosylated nanoparticles namely glyconanoparticles, considered as synthetic mimetics of natural glycoconjugates, paved the way toward diverse biomedical applications. The accessibility of a wide variety of these structured nanosystems, in terms of shapes, sizes, and organized around stable nanoparticles have readily contributed to their development and applications in nanomedicine. In this context, glycosylated gold-nanoparticles (GNPs), glycosylated quantum dots (QDs), fullerenes, single-wall...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Glycodendrimer; Vaccine; Bacteria; Nanoparticles; E. coli; Lanthanides.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700008

Bovine serum albumin-based nanoparticles containing the flavonoid rutin produced by nano spray drying
Provedor de dados: 60
Autores: Pedrozo,Regiellen Cristina; Antônio,Emilli; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
Rutin is a flavonoid compound obtained from different vegetables and fruits; specifically, it is found in the seeds of buckwheat and in fruit peels, particularly citrus. It is also an important constituent of red wine. Rutin exhibits various biological properties including antiviral, vasoprotective, anti-inflammatory, and anticarcinogenic activities. However, its antioxidant activity is the most well studied. Despite the potential for in vitro applications, rutin presents low oral bioavailability that affects its biological activities. Nanoparticles composed of polymers, protein, or lipids are of great importance in the pharmaceutical and nutraceutical areas due to their physicochemical properties, which improve the pharmacokinetics of the drug which is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nano spray drying; Rutin; Nanoparticles; Bovine serum albumin; Antioxidant.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100512

A stability-indicating HPLC-PDA method for the determination of ferulic acid in chitosan-coated poly(lactide-co-glycolide) nanoparticles
Provedor de dados: 60
Autores: Lima,Isabela Angeli de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
ABSTRACT The development and validation of a simple and efficient method for the quantification of ferulic acid in poly (D,L-lactide-co-glycolide) (PLGA) nanoparticles coated with chitosan (CS) by reverse phase high performance liquid chromatography coupled to photodiode array detection was described. For the chromatographic analysis, a reverse phase C-18 column was used, mobile phase consisting of acetonitrile and 0.5% acetic acid (37:63, v/v), isocratically eluted at a flow rate of 1 mL/min. Drug determination was performed at 320 nm. The method was validated in terms of the selectivity, linearity, precision, accuracy, robustness, limits of detection and quantification. The method was linear in the range of 10 to 100 μg/mL (r=0.999) and presented limit...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nanoparticles; Ferulic acid/encapsulation efficiency; Ferulic acid/quantification; Stability; High performance liquid chromatography/validation; PLGA-poly(lactide-co-glycolide)/nanoparticles; Chitosan.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200627

Novel Eudragit® -based polymeric nanoparticles for sustained release of simvastatin
Provedor de dados: 60
Autores: Rodrigues,Deborah Fernandes; Couto,Renê Oliveira do; Sinisterra,Rubén Dario; Jensen,Carlos Eduardo de Matos.
This paper reports on the development of nanoparticles aiming at the in vitro controlled release of simvastatin (SVT). The nanoparticles were prepared by the nanoprecipitation method with polymers Eudragit® FS30D (EDGFS) or Eudragit® NE30D (EDGNE). EDGFS+SVT nanoparticles showed mean size of 148.8 nm and entrapment efficiency of 76.4%, whereas EDGNE+SVT nanoparticles showed mean size of 105.0 nm and entrapment efficiency of 103.2%. Infrared absorption spectra demonstrated that SVT incorporated into the polymer matrix, especially EDGNE. Similarly, the differential scanning calorimeter (DSC) curve presented no endothermic peak of fusion, indicating that the system is amorphous and the drug is not in the crystalline state. The maintenance of SVT in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Simvastatin; Nanoparticles; Poly-methylmethacrylate; Dissolution; Drug delivery systems.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100591

Preparation and characterization of novel, mucoadhesive ofloxacin nanoparticles for ocular drug delivery
Provedor de dados: 60
Autores: Taghe,Shiva; Mirzaeei,Shahla.
The efficacy of conventional ocular formulations is limited by poor corneal retention and permeation, resulting in low ocular bioavailability. Mucoadhesive chitosan (CS)/ tripolyphosphatesodium (TPP) and chitosan (CS)/ tripolyphosphatesodium (TPP)-alginate (ALG) nanoparticles were investigated for the prolonged topical ophthalmic delivery of ofloxacin. A modified ionotropic gelation method was used to produce ofloxacin-loaded nanoreservoir systems. The ofloxacin-loaded CS/TPP and CS/TPP-ALG nanoparticles were characterized for particle size, morphology, zeta potential, encapsulation efficiency, subsequent release and corneal penetration study. The designed nanoparticles have a particle size from 113.8 nm to 509 nm and zeta potential from 16.2 mV to 40.3 mV...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ocular drug delivery; Nanoparticles; Corneal penetration; Ofloxacin; Chitosan.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100519

Development, in vitro biocompatibility, and antitumor efficacy of acetic acid-modified Cordyceps sinensis polysaccharide nanoparticle drug delivery system
Provedor de dados: 60
Autores: Guan,Jiao; Han,Liqin; Shi,Nianqiu; Zhu,Heyun; Wang,Junmin.
Docetaxel-loaded acetic acid conjugated Cordyceps sinensis polysaccharide (DTX-AA-CSP) nanoparticles were prepared through dialysis and their release rates in vitro, particle sizes, zeta potentials, drug loading capacities, and encapsulation efficiencies were characterized for the synthesis of AA-modified CSPs from traditional Chinese medicine Cordyceps sinensis (Berk.) Sacc. Then, the AA-modified CSPs were characterized by 1H-NMR and FT-IR. Furthermore, the biocompatibility of the delivery carrier (AA-CSP nanoparticles) was assessed on human umbilical vein endothelial cells. In vitro antitumor activity studies on DTX-AA-CSP nanoparticles were conducted on the human liver (HepG2) and colon cancer cells (SW480). The DTX-AA-CSP nanoparticles were spherical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Docetaxel; Cordyceps sinensis polysaccharide; Nanoparticles; Antitumor efficacy; Biocompatibility.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100599

Antibacterial, anti fungal and E. coli DNA cleavage of Euphorbia prostrata and Pelargonium graveolens extract and their combination with novel nanoparticles
Provedor de dados: 60
Autores: Dashamiri,Somayeh; Ghaedi,Mehrorang; Naghiha,Reza; Salehi,Amin; Jannesar,Ramin.
As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles. The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with Mn-Ni@Fe3O4-NPs &amp; Mn: Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Aspergillus oryzae, and Candida albicans. The effects of antimicrobial activities on above scenarios were evaluated using disc diffusion, MIC, MBC, and E. coli DNA electrophoresis methods. The results showed that the effects of antibacterial assay values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbia prostrata/extract/antimicrobial activities; Pelargonium graveolens/extract/antimicrobial activities E coli; Candida albicans; Nanoparticles.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400625

Validation of high performance liquid chromatography method for determination of meloxicam loaded PEGylated nanocapsules
Provedor de dados: 60
Autores: Ianiski,Francine Rodrigues; Laporta,Luciane Varini; Rubim,Alexandre Machado; Luchese,Cristiane.
abstract A method to ensure that an analytical method will produce reliable and interpretable information about the sample must first be validated, making sure that the results can be trusted and traced. In this study, we propose to validate an analytical high performance liquid chromatography (HPLC) method for the quantitation of meloxicam loaded PEGylated nanocapsules(M-PEGNC). We performed a validation study, evaluated parameters including specificity, linearity, quantification limit, detection limit, accuracy, precision and robustness. PEGylated nanocapsules were prepared by interfacial deposition of preformed polymer, and the particle size, polydispersity index, zeta potential, pH value and encapsulation efficiency were characterized. The proposed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: High performance liquid chromatography/quantitative analysis; Meloxicam/determination; PEGylated nanocapsules/quality control; Nanoparticles; Poly(ethylene glycol).
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000400823

Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles
Provedor de dados: 60
Autores: Mattos,Ana Cristina de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluorouracil/determination; Nanoparticles; High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/method validation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100013

Synergistic effect of microRNA and albumin-bound nanoparticles for inhibition of glioblastoma cancer cell proliferation
Provedor de dados: 60
Autores: Shariatnasery,Maria; Irani,Shiva; Soleimani,Masoud; Goodarzi,Navid; Dinarvand,Rassoul.
The functional significance of upregulation miR-34a in combination with albumin-bound paclitaxel nanoparticles in U251 glioblastoma cell line has been evaluated. The MTT assay determined that miR-34a and albumin-bound paclitaxel nanoparticles can reduce cell viability, but the combination of both factors has a stronger effect on cell viability. The application of qRT-PCR has demonstrated that the transduction of miR-34a could lead to exogenous upregulation of miR-34a level and downregulation of SURVIVIN. Moreover, treatment of U251 cells with miR-34a and nanoparticles together considerably inhibit SURVIVIN expression compared to miR-34a and nanoparticles alone. Flow cytometry showed that upon miR-34a overexpression cell cycle arrested in G1 phase, while...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MiR-34a; Glioblastoma; Albumin; Paclitaxel; Nanoparticles; Combination therapy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100535

Quality by design (Qbd) approach to develop stability indicating HPLC method for estimation of rutin in chitosan-sodium alginate nanoparticles
Provedor de dados: 60
Autores: Vijayaraj,Surendran; Palei,Narahari N; Archana,Devalapalli; Lathasri,Kuppam; Rajavel,Ponnusamy.
Rutin is a flavonoid glycoside, mainly consists of phenolic compounds, responsible for many biological activities. The objective of the present study was to develop and validate a precise, simple, robust, rapid and reliable reverse phase high -performance liquid chromatography (RP-HPLC) technique by using Qbd approach for evaluating the rutin in nanoparticles. Central composite design (CCD) was employed for optimizing the experimental conditions of RP-HPLC method. Buffer pH, methanol content in the mobile phase composition, flow rate, and wavelength were selected as independent variables whereas retention time, peak area, and asymmetry factor was selected as dependent variables. The retention time, peak area and asymmetric factor of rutin by using...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rutin; Chitosan; Sodium aliginate; Nanoparticles; RP-HPLC; Central composite design; QbD.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100632

Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation
Provedor de dados: 60
Autores: Gatti,Tiago Henrique Honorato; Eloy,Josimar Oliveira; Ferreira,Leonardo Miziara Barboza; Silva,Isabel Cristine da; Pavan,Fernando Rogério; Gremião,Maria Palmira Daflon; Chorilli,Marlus.
Abstract Mucoadhesive nanoparticles are particularly interesting for delivery through nasal or pulmonary routes, as an approach to overcome the mucociliary clearance. Moreover, these nanoparticles are attractive for peptide and protein delivery, particularly for insulin to treat diabetes, as an alternative to conventional parenteral administration. Thus, chitosan, a cationic mucoadhesive polysaccharide found in shells of crustaceans, and the negatively-charged dextran sulfate are able to form nanoparticles through ionic condensation, representing a potential insulin carrier. Herein, chitosan/dextran sulfate nanoparticles at various ratios were prepared for insulin loading. Formulations were characterized for particle size, zeta potential, encapsulation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Insulin; Chitosan; Dextran-Sulfate; Nanoparticles; Mucoadhesion.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100623

Determination of amphotericin B in PLA-PEG blend nanoparticles by HPLC-PDA
Provedor de dados: 60
Autores: Rodrigues,Caroline Danziato; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.
In this work, we developed and validated an effective reversed-phase HPLC method with photodiode array (PDA) detection for the quantitative analysis of amphotericin B (AmB) in poly(lactide)-poly(ethylene glycol) (PLA-PEG) blend nanoparticles. Chromatographic runs were performed on a reverse phase C18 column using a mobile phase comprising a 9% acetic acid and acetonitrile mixture (40:60, v/v) under isocratic elution with a flow rate of 1 mL/min. AmB was detected at a wavelength of 408 nm. The validation process was performed considering the selectivity, linearity, precision, accuracy, robustness, limit of detection (LOD) and limit of quantitation (LOQ) of the method. A concentration range of 1-20 µg/mL was used to construct a linear calibration curve. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amphotericin B/determination; Amphotericin B/encapsulation; High performance liquid chromatography/reverse phase/quantitative analysis; Nanoparticles.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000400859