Consulta: subjectFacets:"Neisseria gonorrhoeae"
Registros recuperados: 9
Data/hora: 07/06/2024 08:00:28

Infección por Neisseria gonorrhoeae y fenotipos de resistencia antimicrobiana, Mar del Plata, 2005-2010
Provedor de dados: 38
Autores: Zotta,Claudio Marcelo; Lavayén,Silvina; Galeano,Griselda; Gianecini,Ricardo; Oviedo,Claudia; Galarza,Patricia.
Se realizó un estudio epidemiológico de tipo descriptivo retrospectivo, para determinar la tasa de infección por Neisseria gonorrhoeae y sus fenotipos de resistencia a los antimicrobianos, en 666 pacientes adultos de ambos sexos que asistieron al servicio al Servicio de ITS del Instituto Nacional de Epidemiología "Dr. Juan H. Jara"- ANLIS "Dr. Carlos G. Malbrán", de la ciudad de Mar del Plata, entre los años 2005-2010. Para el diagnóstico de infección por N. gonorrhoeae, las muestras fueron obtenidas por hisopados endocervicales e hisopados uretrales a varones y luego cultivadas. Los aislamientos fueron remitidos al Laboratorio Nacional de Referencia en ITS para completar el estudio de sensibilidad antimicrobiana. Se obtuvo una tasa de infección por N....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria gonorrhoeae; Infección de transmisión sexual; Tasa de infección; Fenotipos de resistencia; Plásmidos de resistencia.
Ano: 2014
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572014000400010

Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
Provedor de dados: 48
Autores: GARCÍA,KATHERINE P; RUBILAR,PAULINA S; VARGAS,MACARENA F; CÁRDENAS,HUGO; RIOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H ; FUHRER,JUAN; HECKELS,JOHN E; CHRISTODOULIDES,MYRON; VELÁSQUEZ,LUIS A.
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release,...
Tipo: Journal article
Palavras-chave: Cellular damage; Fallopian tubes; Neisseria gonorrhoeae; Nitric oxide.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006

Apoptosis related genes expressed in cultured Fallopian tube epithelial cells infected in vitro with Neisseria gonorrhoeae
Provedor de dados: 48
Autores: REYES,PAZ A; VARGAS,MACARENA F; GARCÍA,KATHERINE P; RUBILAR,PAULINA S; NAVARRETE,PATRICIA A; FUENTES,PAMELA M; RÍOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H; RUBIO,VÍCTOR H; HECKELS,JOHN; CHRISTODOULIDES,MYRON; CÁRDENAS,HUGO; VELASQUEZ,LUIS A.
Background: Infection of the Fallopian tubes (FT) by Neisseria gonorrhoeae (Ngo) can lead to acute salpingitis, an inflammatory condition resulting in damage primarily to the ciliated cells, with loss of ciliary activity and sloughing of the cells from the epithelium. Recently, we have shown that Ngo infection induced apoptosis in FT epithelium cells by a TNF-alpha dependent mechanism that could contribute to the cell and tissue damage observed in gonococcal salpingitis. Aim: To investigate the apoptosis-related genes expressed during apoptosis induction in cultured FT epithelial cells infected in vitro by Ngo. Materials and Methods: In the current study, we used cDNA macroarrays and real time PCR to identify and determine the expression levels of...
Tipo: Journal article
Palavras-chave: Apoptosis; Fallopian tube; Neisseria gonorrhoeae; Salpingitis; TNF-alpha.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400006

Prevalence of Chlamydia trachomatis and Neisseria gonorrhea and associated factors among women living with Human Immunodeficiency Virus in Brazil: a multicenter study
Provedor de dados: 55
Autores: Miranda,Angelica E.; Silveira,Mariangela F.; Travassos,Ana Gabriela; Tenório,Teresinha; Val,Isabel Cristina Chulvis do; Lannoy,Leonor de; Mattos Junior,Hortensio Simões de; Carvalho,Newton Sergio de.
Abstract  Background: Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) cause infections in the female genital tract, increasing susceptibility to and infectiousness of HIV. The objectives of the present study were to determine the prevalence and associated factors of CT and GC infection among HIV-infected women in Brazil.  Methods: Cross-sectional study conducted from March to December 2015, including HIV-infected women attending referral centers in nine states of Brazil, aged 18-49 years, nonpregnant. An interview was conducted including socio-demographic, epidemiological and clinical characteristics. After the interview, gynecological examination was conducted to collect cervical cytology and vaginal secretion to C. trachomatis and N....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlamydia trachomatis; Neisseria gonorrhoeae; HIV; Women; Brazil.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400402

Anogenital infection by Chlamydia trachomatis and Neisseria gonorrhoeae in HIV-infected men and women in Salvador, Brazil
Provedor de dados: 55
Autores: Travassos,Ana Gabriela; Xavier-Souza,Eveline; Netto,Eduardo; Dantas,Eda Vinhaes; Timbó,Maiara; Nóbrega,Isabella; Haguihara,Tatiana; Neumayer,Júlia; Lisboa,Nathalia; Soidan,Maria Angela; Ferreira,Fábio; Brites,Carlos.
ABSTRACT  Background: Infections caused by Chlamydia trachomatis and Neisseria gonorrhoeae are the most common bacterial sexually transmitted infections throughout the world. These sexually transmitted infections are a growing problem in people living with HIV/AIDS. However, the presence of these agents in extra genital sites, remains poorly studied in our country. The objective of this study was to estimate the prevalence of Chlamydia trachomatis and Neisseria gonorrhoeae anal and genital infection in people living with HIV/AIDS followed in a reference center in Salvador, Brazil.  Methods: Cross-sectional study, from June 2013 to June 2015. Proven HIV-infected people attending this reference center were invited. Clinical and epidemiological data were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Chlamydia trachomatis; Neisseria gonorrhoeae; Sexually transmitted infections; Anogenital infections.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600569

Influence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction
Provedor de dados: 56
Autores: Lu,Y.; Rong,C.Z.; Zhao,J.Y.; Lao,X.J.; Xie,L.; Li,S.; Qin,X.
The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Storage time; Chlamydia trachomatis; Ureaplasma urealyticum; Neisseria gonorrhoeae; Stability; QRT-PCR.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000701

Antimicrobial resistance in Neisseria gonorrhoeae: history, molecular mechanisms and epidemiological aspects of an emerging global threat
Provedor de dados: 58
Autores: Costa-Lourenço,Ana Paula Ramalho da; Santos,Késia Thaís Barros dos; Moreira,Beatriz Meurer; Fracalanzza,Sergio Eduardo Longo; Bonelli,Raquel Regina.
ABSTRACT Neisseria gonorrhoeae is the agent of gonorrhea, a sexually transmitted infection with an estimate from The World Health Organization of 78 million new cases in people aged 15-49 worldwide during 2012. If left untreated, complications may include pelvic inflammatory disease and infertility. Antimicrobial treatment is usually effective; however, resistance has emerged successively through various molecular mechanisms for all the regularly used therapeutic agents throughout decades. Detection of antimicrobial susceptibility is currently the most critical aspect for N. gonorrhoeae surveillance, however poorly structured health systems pose difficulties. In this review, we compiled data from worldwide reports regarding epidemiology and antimicrobial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria gonorrhoeae; Surveillance; Resistance mechanisms.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400617

Emergence of fluoroquinolone-resistant Neisseria gonorrhoeae in São Paulo, Brazil
Provedor de dados: 58
Autores: Belda Junior,Walter; Velho,Paulo Eduardo Neves Ferreira; Arnone,Marcelo; Fagundes,Luis Jorge.
Continued monitoring of antimicrobial resistance patterns is essential in order for Sexually Transmitted Diseases (STD) treatment to be effective. Gonococci isolates from 65 patients in São Paulo were submitted to susceptibility testing, and a decreased susceptibility or resistance to ciprofloxacin was observed in 8.7% of these patients, indicating that Neisseria gonorrhoeae fluoroquinolone resistance is emerging in Brazil.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anti-bacterial agents; Ciprofloxacin; Drug resistance; Neisseria gonorrhoeae.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200020

Molecular basis of high-level ciprofloxacin resistance in Neisseria gonorrhoeae strains from Shandong Province, China
Provedor de dados: 58
Autores: Zhao,L.H.; Zhao,S.P.
In the study, the ciprofloxacin resistance rate was 100%. High-level ciprofloxacin resistance rate was 63.55%. Sixteen different mutation patterns involved in the formation of ciprofloxacin resistance were identified. The most prevalent were patterns P7 (25.2%), P8 (15.0%), P9 (11.2%), P1 (10.3%), and P5 (10.3%). All of the 107 NG isolates analyzed for mutations in the study have demonstrated a change of Ser-91 → Phe in the gyrA gene, and all except one have demonstrated a change in position 95 of the amino acid sequence. All of the 68 high-level QRNG isolates had double mutations in gyrA gene combined with a single or two mutations in parC gene. It is most important that a new mutation site of Ile-97 → Met in gyrA and a new mutation of Leu-106 → Ile in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neisseria gonorrhoeae; Ciprofloxacin resistance; High-level; Molecular analysis.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100039