Consulta: subjectFacets:"Neuroprotection"
Registros recuperados: 15
Data/hora: 10/06/2024 09:17:36

Analysis of the NMDA in Focal Cerebral Ischemia in Rats
Provedor de dados: 77
Autores: Tirapelli,D. P. C; Carlotti Jr,C. G; Leite,J. P; Lizarte,F. S. N; Tirapelli,L. F; Colli,B. O.
NMDAR (N-methyl-D-aspartate receptor) is one subtype of ionotrophic glutamate receptor which is extensively distributed in the central nervous system (CNS). In the mammalian CNS, NMDAR serves prominent roles in the pathophysiologic process of cerebral ischemia. This study aimed to investigate the pattern of expression of protein and gene of the excitatory neurotransmitter NMDAR in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. 120 rats were randomly divided into 6 groups (20 animals each): control - no surgery; sham - simulation of surgery; ischemic - focal ischemia for 1 hour, without reperfusion; ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic +...
Tipo: Journal article
Palavras-chave: NMDA expression; Cerebral ischemia; Neuroprotection; Hypothermia and ketoprofen.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300035

Assessment of neuropharmacological potential of low molecular weight components extracted from Rhinella schneideri toad poison
Provedor de dados: 80
Autores: Baldo,Mateus Amaral; Cunha,Alexandra Olimpio Siqueira; Godoy,Lívea Dornela; Liberato,José Luiz; Yoneda,Juliana Sakamoto; Fornari-Baldo,Elisa Correa; Ciancaglini,Pietro; Santos,Wagner Ferreira dos; Arantes,Eliane Candiani.
Abstract  Background: Studies on toad poison are relevant since they are considered a good source of toxins that act on different biological systems. Among the molecules found in the toad poison, it can be highlighted the cardiotonic heterosides, which have a known mechanism that inhibit Na+/K+-ATPase enzyme. However, these poisons have many other molecules that may have important biological actions. Therefore, this work evaluated the action of the low molecular weight components from Rhinella schneideri toad poison on Na+/K+-ATPase and their anticonvulsive and / or neurotoxic effects, in order to detect molecules with actions of biotechnological interest.  Methods: Rhinella schneideri toad (male and female) poison was collected by pressuring their...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rhinella schneideri; Toad poison; Bufadienolides; Seizures; Neuroprotection.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100309

Neuroprotective properties of RT10, a fraction isolated from Parawixia bistriata spider venom, against excitotoxicity injury in neuron-glia cultures
Provedor de dados: 80
Autores: Primini,Eduardo Octaviano; Liberato,José Luiz; Fontana,Andreia Cristina Karklin; Santos,Wagner Ferreira dos.
Abstract  Background: L-Glutamate (L-Glu), the major excitatory neurotransmitter in the mammalian Central Nervous System (CNS), is essential to cognitive functions. However, when L-Glu is accumulated in large concentrations at the synaptic cleft, it can induce excitotoxicity that results in secondary damage implicated in many neurological disorders. Current therapies for the treatment of neurological disorders are ineffective and have side effects associated with their use; therefore, there is a need to develop novel treatments. In this regard, previous studies have shown that neuroactive compounds obtained from the venom of the spider Parawixia bistriata have neuroprotective effects in vitro and in vivo. In this sense, this work aimed to evaluate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-Glutamate; Excitotoxicity; Neuroprotection; Parawixia bistriata; RT10.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100311

Grape juice increases the BDNF levels but not alter the S100B levels in hippocampus and frontal cortex from male Wistar Rats
Provedor de dados: 42
Autores: DANI,CAROLINE; ANDREAZZA,ANA CRISTINA; GONÇALVES,CARLOS ALBERTO; KAPIZINSKI,FLÁVIO; HENRIQUES,JOÃO A. P.; SALVADOR,MIRIAN.
ABSTRACT Several studies have shown that a high consumption of vegetables and fruits is consistently associated with a low risk of oxidative stress-induced diseases, which includes some degenerative diseases such as amyotrophic lateral sclerosis, Alzheimer and Parkinson. Therefore, the objective of this study is to verify the effects of conventional and organic grape juice in the modulation of the neurotrophic factor (BDNF) and astrocytic markers protein (S100B) in hippocampus and frontal cortex of Wistar rats. In this study, 24 male Wistar rats were divided into three groups. To the first one, it was given organic purple grape juice; to the second, conventional grape juice, while the last one received only saline. After 30 days, all rats were sacrificed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Brain; Grape juice; Neuroprotection; Polyphenol.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100155

Agmatine pretreatment protects retinal ganglion cells (RGC-5 cell line) from oxidative stress in vitro
Provedor de dados: 47
Autores: Yoko,Iizuka; Hong,Samin; Yun Kim,Chan; Kab Kim,Seung; Je Seong,Gong.
Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 &amp;#956;M) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick end-labeling (TUNEL)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuronal damage; Apoptosis; Neuroprotection.
Ano: 2008
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300005

Novel neurotrophic factor secreted by amniotic epithelial cells
Provedor de dados: 47
Autores: Venkatachalam,Sankar; Palaniappan,Tamilselvi; Jayapal,Prem Kumar; Neelamegan,Sridharan; Rajan,Sridhar Skylab; Muthiah,Vijaya Prakash Krishnan.
By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. Amniotic epithelial cells were believed to secrete an "Epidermal Growth Factor (EGF) - like" factor and exact identification was not made. At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroprotection; Neural retina development; FGF-2; Neurotrophism; Placental stem cells.
Ano: 2009
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200002

Magnesium chloride alone or in combination with diazepam fails to prevent hippocampal damage following transient forebrain ischemia
Provedor de dados: 56
Autores: Milani,H.; Lepri,E.R.; Giordani,F.; Favero-Filho,L.A.
In the central nervous system, magnesium ion (Mg2+) acts as an endogenous modulator of N-methyl-D-aspartate (NMDA)-coupled calcium channels, and may play a major role in the pathomechanisms of ischemic brain damage. In the present study, we investigated the effects of magnesium chloride (MgCl2, 2.5, 5.0 or 7.5 mmol/kg), either alone or in combination with diazepam (DZ), on ischemia-induced hippocampal cell death. Male Wistar rats (250-300 g) were subjected to transient forebrain ischemia for 15 min using the 4-vessel occlusion model. MgCl2 was applied systemically (sc) in single (1x, 2 h post-ischemia) or multiple doses (4x, 1, 2, 24 and 48 h post-ischemia). DZ was always given twice, at 1 and 2 h post-ischemia. Thus, ischemia-subjected rats were assigned...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerebral ischemia; Hippocampal lesion; CA1 cell loss; Magnesium chloride; Diazepam; Neuroprotection.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000016

Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning
Provedor de dados: 56
Autores: Cui,S.Q.; Wang,Q.; Zheng,Y.; Xiao,B.; Sun,H.W.; Gu,X.L.; Zhang,Y.C.; Fu,C.H.; Dong,P.X.; Wang,X.M.
We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Puerarin; Chronic alcohol poisoning; Neuroprotection; Microglia; Inflammatory factors.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600515

Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector
Provedor de dados: 56
Autores: Petrs-Silva,H.; Chiodo,V.; Chiarini,L.B.; Hauswirth,W.W.; Linden,R.
Exclusion of the transcription factor Max from the nucleus of retinal ganglion cells is an early, caspase-independent event of programmed cell death following damage to the optic axons. To test whether the loss of nuclear Max leads to a reduction in neuroprotection, we developed a procedure to overexpress Max protein in rat retinal tissue in vivo. A recombinant adeno-associated viral vector (rAAV) containing the max gene was constructed, and its efficiency was confirmed by transduction of HEK-293 cells. Retinal ganglion cells were accessed in vivo through intravitreal injections of the vector in rats. Overexpression of Max in ganglion cells was detected by immunohistochemistry at 2 weeks following rAAV injection. In retinal explants, the preparation of...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Retina; Max expression; Transcription factor; Viral vector; Gene therapy; Neuroprotection.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300008

Neuroprotection by flavonoids
Provedor de dados: 56
Autores: Dajas,F.; Rivera-Megret,F.; Blasina,F.; Arredondo,F.; Abin-Carriquiry,J.A.; Costa,G.; Echeverry,C.; Lafon,L.; Heizen,H.; Ferreira,M.; Morquio,A.
The high morbidity, high socioeconomic costs and lack of specific treatments are key factors that define the relevance of brain pathology for human health and the importance of research on neuronal protective agents. Epidemiological studies have shown beneficial effects of flavonoids on arteriosclerosis-related pathology in general and neurodegeneration in particular. Flavonoids can protect the brain by their ability to modulate intracellular signals promoting cellular survival. Quercetin and structurally related flavonoids (myricetin, fisetin, luteolin) showed a marked cytoprotective capacity in in vitro experimental conditions in models of predominantly apoptotic death such as that induced by medium concentrations (200 µM) of H2O2 added to PC12 cells in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flavonoids; Quercetin; Focal ischemia; Neuroprotection.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200002

ERKs and mitochondria-related pathways are essential for glycyrrhizic acid-mediated neuroprotection against glutamate-induced toxicity in differentiated PC12 cells
Provedor de dados: 56
Autores: Wang,D.; Guo,T.Q.; Wang,Z.Y.; Lu,J.H.; Liu,D.P.; Meng,Q.F.; Xie,J.; Zhang,X.L.; Liu,Y.; Teng,L.S.
The present study focuses on the neuroprotective effect of glycyrrhizic acid (GA, a major compound separated from Glycyrrhiza Radix, which is a crude Chinese traditional drug) against glutamate-induced cytotoxicity in differentiated PC12 (DPC12) cells. The results showed that GA treatment improved cell viability and ameliorated abnormal glutamate-induced alterations in mitochondria in DPC12 cells. GA reversed glutamate-suppressed B-cell lymphoma 2 levels, inhibited glutamate-enhanced expressions of Bax and cleaved caspase 3, and reduced cytochrome C (Cyto C) release. Exposure to glutamate strongly inhibited phosphorylation of AKT (protein kinase B) and extracellular signal-regulated kinases (ERKs); however, GA pretreatment enhanced activation of ERKs but...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycyrrhizic acid; Neuroprotection; Glutamate; ERKs; Mitochondria.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900773

Retinal protective effects of topically administered agmatine on ischemic ocular injury caused by transient occlusion of the ophthalmic artery
Provedor de dados: 56
Autores: Hong,S.; Hara,H.; Shimazawa,M.; Hyakkoku,K.; Kim,C.Y.; Seong,G.J.
Agmatine, an endogenous polyamine and putative neuromodulator, is known to have neuroprotective effects on various neurons in the central nervous system. We determined whether or not topically administered agmatine could reduce ischemic retinal injury. Transient ocular ischemia was achieved by intraluminal occlusion of the middle cerebral artery of ddY mice (30-35 g) for 2 h, which is known to also induce occlusion of the ophthalmic artery. In the agmatine group (N = 6), a 1.0 mM agmatine-containing ophthalmic solution was administered four times daily for 2 weeks before occlusion. In the control group (N = 6), a 0.1% hyaluronic acid ophthalmic solution was instilled at the same times. At 22 h after reperfusion, the eyeballs were enucleated and the retinal...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Apoptosis; Ischemia; Neuroprotection; Retina.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300005

Pharmacology and toxicology of diphenyl diselenide in several biological models
Provedor de dados: 56
Autores: Rosa,R.M.; Roesler,R.; Braga,A.L.; Saffi,J.; Henriques,J.A.P.
The pharmacology of synthetic organoselenium compounds indicates that they can be used as antioxidants, enzyme inhibitors, neuroprotectors, anti-tumor and anti-infectious agents, and immunomodulators. In this review, we focus on the effects of diphenyl diselenide (DPDS) in various biological model organisms. DPDS possesses antioxidant activity, confirmed in several in vitro and in vivo systems, and thus has a protective effect against hepatic, renal and gastric injuries, in addition to its neuroprotective activity. The activity of the compound on the central nervous system has been studied since DPDS has lipophilic characteristics, increasing adenylyl cyclase activity and inhibiting glutamate and MK-801 binding to rat synaptic membranes. Systemic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diphenyl diselenide; Organoselenium; Antioxidants; Neuroprotection; Saccharomyces cerevisiae; Mutagenesis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000001

Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress
Provedor de dados: 56
Autores: Iizuka,Y.; Hong,S.; Kim,C.Y.; Yang,W.I.; Lee,J.E.; Seong,G.J.
Agmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of agmatine using transformed RGCs (RGC-5 cell line). Differentiated RGC-5 cells were pretreated with 100 μM agmatine and consecutively exposed to 1.0 mM hydrogen peroxide (H2O2). Cell viability was determined by measuring lactate dehydrogenase (LDH), and the effects of selective alpha 2-adrenergic receptor antagonist yohimbine (0-500 nM) and N-methyl-D-aspartic acid (NMDA) receptor agonist NMDA (0-100 µM) were evaluated. Agmatine’s protective effect was compared to...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Agmatine; Neuroprotection; Oxidative stress; Retinal ganglion cell.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006

Neuroprotective and MAOB inhibitory effects of a series of caffeine-8-thioglycolic acid amides
Provedor de dados: 60
Autores: Kasabova-Angelova,Alexandra; Kondeva-Burdina,Magdalena; Mitkov,Javor; Georgieva,Maya; Tzankova,Virginia; Zlatkov,Alexander.
The effects of new derivatives of caffeine-8-thioglycolic acid (100 µM) on isolated rat brain synaptosomes, human neuroblastoma cell line SH-SY5Y and human recombinant MAOB enzyme (hMAOB) (1 µM) were evaluated. Most of the compounds, administered alone, didn’t show statistically significant neurotoxic effects on SH-SY5Y, when compared to the control (non-treated cells). Of all studied structures JTA-2Ox, JTA-11, JTA-12 and JTA-13 decreased cell viability. In combination with 6-hydroxydopamine (6-OHDA) (100 µM), only JTA-1 and JTA-2 revealed neuroprotective effects, stronger than those of caffeine. All compounds administered alone revealed, neurotoxic effects on synaptosomes, as compared to non-treated synaptosomes. JTA-1, JTA-2 and JTA-3 showed lowest...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neuroprotection; Caffeine; Synaptosomes; SH-SY5Y; HMAOB.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100529