Consulta: subjectFacets:"Neutralization"
Registros recuperados: 16
Data/hora: 01/06/2024 01:08:38

Sistemas de Integração Lavoura-Pecuária-Floresta como estratégia para neutralização da emissão de metano entérico de bovinos na região do Cerrado de Minas Gerais.
Provedor de dados: 14
Autores: CAMPANHA, M. M.; COSTA, T. C. e C. da; GONTIJO NETO, M. M.; RESENDE, A. V. de; BORGHI, E.
Este trabalho foi desenvolvido com o objetivo de avaliar o potencial de neutralização das emissões de metano entérico de bovinos em pasto, pela fixação de CO2 no componente arbóreo de diferentes modelos para recuperação de pastagens degradadas no Cerrado de Minas Gerais, com diferentes níveis de investimento tecnológico em sistemas de Integração Lavoura-Pecuária-Floresta (ILPF).
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Sistema de Cultivo; Metano; Neutralização; Pastagem; Methane; Neutralization; Pastures.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1135355

Curvas de neutralização dos principais solos de Rondônia.
Provedor de dados: 14
Autores: LOURENCO, R. S.
Foram selecionados metodos mais adequados na determinacao da necessidade de calcario para correcao da acidez desses solos. Dezenove solos de Rondonia foram incubados com doses crescentes de CaCO3, equivalente a 0,2,4,6,8,10,12,14,16,18 e 20 t/ha, para estabelecer curvas de neutralizacao dos solos mais representativos do Estado e comparar os metodos de recomposicao de calagem: incubacao, CTC, SMP, Al+++ x 2, A+++ x2, + 2 ou 3 - (Ca++ + Mg++). O metodo de incubacao mostrou-se propicio em solos com teores elevados de aluminio trocavel, no entanto, as recomendacoes obtidas sao relativamente proximas, quando comparadas com os demais metodos. O metodo do Al+++ x 2, apresentou certa limitacao, mais, em determinados casos e vantajoso. Destarte, a escolha do metodo...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Reacao; Neutralidade; Brasil; Rondonia.; Solo.; Brazil; Neutralization; Soil.
Ano: 1984
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/697949

Determinação de cálcio e magnésio em pó de rochas complexas utilizando a titulação com EDTA.
Provedor de dados: 14
Autores: DIAS, D. A. S.; FALEIRO, A. S. G.; ANDRADE, L. R. M. de; MARTINS, E. de S.; LINHARES, N. W.; NASCIMENTO, M. T. do; SOUZA, A. L. de.
bitstream/CPAC-2009/26843/1/p2005_44.pdf
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E)
Palavras-chave: Correção do solo; Rock.; Acidez do Solo; Neutralização; Poder Neutralizante; Rocha.; Neutralization; Soil amendments; Soil pH.
Ano: 2006
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/569459

Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
Provedor de dados: 79
Autores: Oshima-Franco,Y.; Leite,G. B.; Valério,A. A.; Hyslop,S.; Andriao-Escarso,S.; Giglio,J. R.; Prado-Franceschi,J.; Cruz-Höfling,M. A.; Rodrigues-Simioni,L.
Bothrops jararacussu venom and its major toxin bothropstoxin-I (BthTX-I) possess myotoxic and neurotoxic properties. The efficacy of a rabbit antivenom raised against B. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin BthTX-I was studied in mouse isolated phrenic nerve-diaphragm (PND) and extensor digitorum longus (EDL) preparations. The times required for 50% neuromuscular blockade in PND and EDL preparations for venom were 70+11.5 (S.E.M., n=5) min and 58+8 (n=16) (50 mu g/mL), and for BthTX-I 31+6 (n=3) min and 30+3 (n=5) min (20 mu g/mL), respectively. After 120 min incubation, creatine kinase (CK) concentrations in solution containing the EDL preparations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops venom; Myotoxin; Neutralization; Neuromuscular junction; Histological analysis; Bothrops jararacussu.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200004

IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Provedor de dados: 80
Autores: Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC.
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008

Heparin and commercial bothropic antivenom against the paralyzing effect of Bothrops jararacussu snake venom
Provedor de dados: 80
Autores: Rostelato-Ferreira,S; Rodrigues-Simioni,L; Oshima-Franco,Y.
The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols: Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50% neuromuscular blockade...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararacussu; Heparin; Neutralization; Nerve-muscle preparation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100005

Proteomics and antivenom immunoprofiling of Russell’s viper (Daboia siamensis) venoms from Thailand and Indonesia
Provedor de dados: 80
Autores: Lingam,Thava Malar Changra; Tan,Kae Yi; Tan,Choo Hock.
Background The Eastern Russell’s viper, Daboia siamensis, is a WHO Category 1 medically important venomous snake. It has a wide but disjunct distribution in Southeast Asia. The specific antivenom, D. siamensis Monovalent Antivenom (DsMAV-Thailand) is produced in Thailand but not available in Indonesia, where a heterologous trivalent antivenom, Serum Anti Bisa Ular (SABU), is used instead. This study aimed to investigate the geographical venom variation of D. siamensis from Thailand (Ds-Thailand) and Indonesia (Ds-Indonesia), and the immunorecognition of the venom proteins by antivenoms.  Methods:  The venom proteins were decomplexed with reverse-phase high-performance liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Venomics; Antivenomics; Eastern Russell's viper; Neutralization.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100302

Interval between first dose and booster affected antibody production in cattle vaccinated against rabies
Provedor de dados: 80
Autores: Albas,A.; Fontolan,O. L.; Pardo,P. E.; Bremer Neto,H.; Sartori,A.
In this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three alternative immunization procedures. Forty-five bovines (breed nelore) were then organized in three groups (A, B and C, with 15 animals/group). Group A received only one vaccine dose at day zero and Group B received the first dose at day zero and then another dose at day 30 (early booster). Group C was also immunized with two doses; however, the booster was postponed until day 180 after the first dose (delayed booster). Blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390, and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. The protocol used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Vaccine; Cattle; Antibody; Neutralization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300010

Enzymatic and immunological properties of Bungarus flaviceps (red-headed krait) venom
Provedor de dados: 80
Autores: Tan,NH; Fung,SY; Ponnudurai,G.
Bungarus flaviceps (red-headed krait) venom presents an intravenous LD50 of 0.32 μg/g and exhibits enzymatic activities similar to other Bungarus toxins. ELISA cross-reactions between anti-Bungarus flaviceps and a variety of elapid and viperid venoms were observed in the current study. Double-sandwich ELISA was highly specific, since anti-B. flaviceps serum did not cross-react with any tested venom, indicating that this assay can be used for species diagnosis in B. flaviceps bites. In the indirect ELISA, anti-B. flaviceps serum cross-reacted moderately with three different Bungarus venoms (9-18%) and Notechis scutatus venom, but minimally with other elapid and viperid toxins. The results indicated that B. flaviceps venom shares common epitopes with other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bungarus flaviceps venom; Enzymes; ELISA; Neutralization.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100014

Influence of processing parameters on physicochemical properties of  Iranian mulberry (Morusalba L.) molasses
Provedor de dados: 111
Autores: Basiri, Shadi.
The white mulberry is a native fruit to China. The white mulberry as a fruit with high water content and sensitive texture, is a perishable fruit with high-level waste.  To reduce the losses of the white mulberry, it used to be processed traditional dried fruit and mulberry molasses. The purpose of this study was to determine chemical and physical properties of white mulberry molasess made up of different cultivars, neutralizers and concentration methods. Two examples of cultivars were Bokhara and Khardar mulberry (local cultivars), two neutralizing compounds were white soil (traditionally special soil can be used as neutralization) and calcium carbonate, the concentration conditions were concentration under vacuum conditions and concentration under...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acidity; Concentration; Mulberry molasses; Neutralization.
Ano: 2016
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3441

Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine
Provedor de dados: 52
Autores: Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002

Neutralization of the edema-forming, defibrinating and coagulant effects of Bothrops asper venom by extracts of plants used by healers in Colombia
Provedor de dados: 56
Autores: Núñez,V.; Otero,R.; Barona,J.; Saldarriaga,M.; Osorio,R.G.; Fonnegra,R.; Jiménez,S.L.; Díaz,A.; Quintana,J.C.
We determined the neutralizing activity of 12 ethanolic extracts of plants against the edema-forming, defibrinating and coagulant effects of Bothrops asper venom in Swiss Webster mice. The material used consisted of the leaves and branches of Bixa orellana (Bixaceae), Ficus nymphaeifolia (Moraceae), Struthanthus orbicularis (Loranthaceae) and Gonzalagunia panamensis (Rubiaceae); the stem barks of Brownea rosademonte (Caesalpiniaceae) and Tabebuia rosea (Bignoniaceae); the whole plant of Pleopeltis percussa (Polypodiaceae) and Trichomanes elegans (Hymenophyllaceae); rhizomes of Renealmia alpinia (Zingiberaceae), Heliconia curtispatha (Heliconiaceae) and Dracontium croatii (Araceae), and the ripe fruit of Citrus limon (Rutaceae). After preincubation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutralization; Edema; Defibrination; Coagulation; Bothrops asper venom; Plant extracts.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700005

HIV-1 binding and neutralizing antibodies of injecting drug users
Provedor de dados: 56
Autores: Ouverney,E.P.; Teixeira,S.L.M.; Silva-de-Jesus,C.; Hacker,M.A.; Morgado,M.G.; Bastos,F.I.; Bongertz,V.
Previous studies have demonstrated a stronger seroreactivity against some synthetic peptides responsible for inducing neutralizing antibodies in injecting drug users (IDU) compared to that of individuals sexually infected with HIV-1 (S), but the effectiveness in terms of the neutralizing ability of these antibodies has not been evaluated. Our objective was to study the humoral immune response of IDU by determining the specificity of their antibodies and the presence of neutralizing antibodies. The neutralization capacity against the HIV-1 isolate MN (genotype B), the primary HIV-1 isolate 95BRRJ021 (genotype F), and the seroreactivity with peptides known to induce neutralizing antibodies, from the V2 and V3 loops of different HIV-1 subtypes, were analyzed....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; Injecting drug users; Antibodies; Neutralization.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900004

Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms
Provedor de dados: 56
Autores: Picolo,G.; Chacur,M.; Gutiérrez,J.M.; Teixeira,C.F.P.; Cury,Y.
Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent antivenom produced at Instituto Clodomiro Picado (AVCP, 1 ml neutralizes 2.5 mg B. aspar venom). The intraplantar injection of B. jararaca and B. asper venoms caused hyperalgesia, which peaked 1 and 2 h after injection, respectively. Both venoms also induced edema with a similar time course. When neutralization assays involving the independent injection of venom and antivenom were performed, the hyperalgesia induced by B. jararaca venom was neutralized only when bothropic antivenom was administered iv 15 min before venom injection, whereas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bothrops jararaca venom; Bothrops asper venom; Hyperalgesia; Edema; Antivenom; Neutralization.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000016

N-terminal amino acids of bovine alpha interferons are relevant for the neutralization of their antiviral activity
Provedor de dados: 56
Autores: Barreto Filho,J.B.; Eiras,P.R.S.; Golgher,R.R.
The structure-function relationship of interferons (IFNs) has been studied by epitope mapping. Epitopes of bovine IFNs, however, are practically unknown, despite their importance in virus infections and in the maternal recognition of pregnancy. It has been shown that recombinant bovine (rBo)IFN-alphaC and rBoIFN-alpha1 differ only in 12 amino acids and that the F12 monoclonal antibody (mAb) binds to a linear sequence of residues 10 to 34. We show here that the antiviral activities of these two IFNs were neutralized by the F12 mAb to different extents using two tests. In residual activity tests the antiviral activity dropped by more than 99% with rBoIFN-alphaC and by 84% with rBoIFN-alpha1. In checkerboard antibody titrations, the F12 mAb titer was 12,000...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bovine alpha interferon; Neutralization; Amino acid; Epitope.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500015

Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: comparison with two commercial antivenoms
Provedor de dados: 56
Three horse-derived antivenoms were tested for their ability to neutralize lethal, hemorrhagic, edema-forming, defibrinating and myotoxic activities induced by the venom of Bothrops atrox from Antioquia and Chocó (Colombia). The following antivenoms were used: a) polyvalent (crotaline) antivenom produced by Instituto Clodomiro Picado (Costa Rica), b) monovalent antibothropic antivenom produced by Instituto Nacional de Salud-INS (Bogotá), and c) a new monovalent anti-B. atrox antivenom produced with the venom of B. atrox from Antioquia and Chocó. The three antivenoms neutralized all toxic activities tested albeit with different potencies. The new monovalent anti-B. atrox antivenom showed the highest neutralizing ability against edema-forming and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Bothrops atrox; Snake venom; Antivenom; Neutralization; Antioquia; Chocó.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300011