Consulta: subjectFacets:"Next-generation sequencing (NGS)"
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Data/hora: 17/06/2024 20:30:10

A comparison between SOLiD 5500XLand Ion Torrent PGM-derived miRNA expression profiles in two breast cell lines
Provedor de dados: 74
Autores: Branco,Gabriela Pereira; Valieris,Renan; Povoa,Lucas Venezian; Araújo,Luiza Ferreira de; Fernandes,Gustavo Ribeiro; Souza,Jorge Estefano Santana de; Amorim,Maria Galli de; Ferreira,Elisa Napolitano e; Silva,Israel Tojal da; Nunes,Diana Noronha; Dias-Neto,Emmanuel.
Abstract Next-generation sequencing (NGS) platforms allow the analysis of hundreds of millions of molecules in a single sequencing run, revolutionizing many research areas. NGS-based microRNA studies enable expression quantification in unprecedented scale without the limitations of closed-platforms. Yet, whereas a massive amount of data produced by these platforms is available, comparisons of quantification/discovery capabilities between platforms are still lacking. Here we compare two NGS-platforms: SOLiD and PGM, by evaluating their microRNA identification/quantification capabilities using two breast-derived cell-lines. A high expression correlation (R2 &gt; 0.9) was achieved, encompassing 97% of the miRNAs, and the few discrepancies in miRNA counts were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Next-generation sequencing (NGS); MiRNA expression profiles; SOLiD; PGM.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400802