Consulta: subjectFacets:"Nitric oxide"
Registros recuperados: 143
Data/hora: 06/06/2024 01:01:42

NO AND N2O FLUXES FROM A BELGIAN FOREST AFFECTED BY ELEVATED NITROGEN DEPOSITION
Provedor de dados: 73
Autores: Boeckx,Pascal; Vervaet,Hilde; Cleemput,Oswald van.
Nitrous oxide (N2O) and nitric oxide (NO) are important atmospheric trace gases, and soils (including forest soils) are a substantial source of both gases. In forests subjected to elevated N deposition these emissions are considered as indirect emissions from agricultural N sources (mainly NH3) or combustion (e.g. NOx from traffic). However, knowledge about N2O and NO exchange between forests and the atmosphere is scarce. The aim of this study was to determine N2O and NO emission rates from a deciduous forest in Belgium, receiving a high N-deposition (ca. 40 kg N ha-1 yr-1). In April 2000, the NO emission from the forested location was 17.3 (± 1.2) and 17.05 (± 0.7) ng NO-N m-2 s-1, respectively, for the soil including the organic layer and the mineral...
Tipo: Journal article
Palavras-chave: Emission; Forest soil; Nitrogen; Cycling; Nitric oxide; Nitrous oxide.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432005000200003

Genotoxic evaluation of sodium nitroprusside in Aspergillus nidulans
Provedor de dados: 74
Autores: Chiuchetta,Simone Jurema Ruggeri; Castro-Prado,Marialba Avezum Alves de.
The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH+//uvsH+) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 muM, 80 muM and 160 muM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Parasexual cycle; Homozygotization index; Conidiation; Mitotic recombination.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500023

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio.
Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland....
Tipo: Text
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf

The impact of insecticide resistance on Culex pipiens immunity
Provedor de dados: 5
Autores: Vezilier, Julien; Nicot, Antoine; De Lorgeril, Julien; Gandon, Sylvain; Rivero, Ana.
Because of their role as vectors of diseases, the evolution of insecticide resistance in mosquitoes has been intensively investigated. Insecticide resistance is associated to a wide range of pleiotropic effects on several key life-history traits of mosquitoes such as longevity and behavior. However, despite its potential implications in pathogen transmission, the effects of insecticide resistance on mosquito immunity have received little, if any, attention. Here, we investigate the impact of insecticide resistance in Culex pipiens, an epidemiologically important vector of a wide array of pathogens. Using both isogenic laboratory strains and field-caught mosquitoes, we investigate the impact of two main insecticide resistance mechanisms (metabolic...
Tipo: Text
Palavras-chave: Acetylcholinesterase; Anopheles; Antimicrobial peptide; Carboxylesterases; Nitric oxide; Transferrin.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00136/24773/22845.pdf

Adiponectin Effect on Nitric Oxide Secretion by Normal and Endometriotic Human Endometrial Stromal Cells: in vitro Study
Provedor de dados: 77
Autores: Bohlouli,Somayeh; Khazaei,Mozafar; Rabzia,Arezou; Khazaei,Mohammad Rasool; Sadeghi,Ehsan.
Endometriosis is an estrogen-dependent disease in reproductive age women. Adiponectin and Nitric oxide (NO) have an important role in physiologic functions especially in human reproductive system. Levels of NO increased in the endometriosis patients but serum adiponectin levels decreased in woman with endometriosis. The aim of this study was to determine adiponectin effect on nitric oxide secretion by cultured normal and endometriotic human endometrial stromal cells. In this experimental study, normal (n= 10) and endometriotic endometrial biopsies (n= 10) were taken in sterile condition. Stromal cells isolated and cultured in in DMEM/ F12 medium and treated with adiponectin concentrations (0, 10, 100, and 200 ng/ml) for 24 and 48 hours. NO assay was done...
Tipo: Journal article
Palavras-chave: Adiponectin; Nitric oxide; Endometriosis; Stromal cell.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000100053

ENALAPRIL AND VERAPAMIL ATTENUATE THE AORTIC WALL REMODELING IN NITRIC OXIDE DEFICIENCY
Provedor de dados: 77
Autores: Meirelles Pereira,Leila Maria; Gonçalves Bezerra,Daniele; Mandarim-de-Lacerda,Carlos Alberto.
Forty mature male rats (Wistar strain) were maintained alive for 40 days, separated in four groups of five rats each (Control, L-NAME, L-NAME + Enalapril, L-NAME + Verapamil). Three aortic wall parameters were analyzed, as follows: the Q A(SMN) (the number of the tunica media smooth muscle nuclear profiles per area), the S V(lamellae) (surface density of the aortic lamellae), and the aortic wall thickness (AWT). Rats of the L-NAME group showed high blood pressure and cardiac hypertrophy. The AWT, the Q A(SMN) and the S V(lamellae) showed the same tendency among the groups. The increased AWT, or the reduced Q A(SMN) and the S V(lamellae) in l-NAME rats were not completely prevented by the administration of enalapril or verapamil. Hypertension causes the AWT...
Tipo: Journal article
Palavras-chave: Nitric oxide; Aorta; Enalapril; Verapamil; Stereology.
Ano: 2003
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000100002

Immunomodulatory action of propolis on macrophage activation
Provedor de dados: 79
Autores: ORSI,R.O.; FUNARI,S.R.C.; SOARES,A.M.V.C.; CALVI,S.A.; OLIVEIRA,S.L.; SFORCIN,J.M.; BANKOVA,V.
Propolis has been the subject of several recent studies, with the aim of elucidating its biological and pharmacological properties. Propolis has a well-known antimicrobial activity as well as antioxidant, antitumoral, antiinflammatory, and regenerative properties, but literature about its effects on the immune response is scarce. The goal of this work was to evaluate the propolis effect on macrophage activation by oxygen (H2O2) and nitrogen (NO) metabolite determination. Propolis was produced by africanized honeybees and hydroalcoholic solutions were prepared at different concentrations. Peritoneal macrophages were obtained from male BALB/c mice and culture cells were stimulated in vitro with propolis or interferon-gamma (IFN-gamma). In the in vivo assay,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Propolis; Macrophages; Hydrogen peroxide; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200006

Nitric Oxide and Luteal Blood Flow in the Luteolytic Cascade in the Cow
Provedor de dados: 19
Autores: SHIRASUNA, Koumei; 白砂, 孔明.
SRD Young Investigator Award 2009
Palavras-chave: Corpus luteum; Luteal blood flow; Luteolysis; Nitric oxide.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2823

NITRIC OXIDE IS INVOLVED IN EARLY HOST DEFENSE AGAINST A PRIMARY INFECTION WITH BABESIA MICROTI IN MICE
Provedor de dados: 19
Autores: Remer, Katharina; Igarashi, Ikuo; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Babesia microti; Nitric oxide; INOS-/- mice; Aminoguanidine; Parasitemia; Hematocrit; Interferon-γ.
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/633

Increased iNOS gene expression in the granulosa layer of F1 follicle of over-fed and under-fed broiler breeder hens
Provedor de dados: 89
Autores: Sheikh Ahmadi,A; Zaghari,M; Shivazad,M; Hassanpour,H; Towhidi,A.
To clarify the effects of high (20 and 40% more than normal) and low (20% less than normal) daily feed allowance on egg and body parameters, ovarian morphology and plasma glucose, cholesterol, triacylglycerol, leptin-like hormone, nitrite/nitrate and gene expression of inducible nitric oxide synthase (iNOS) in the granulosa layer of F1 follicle, broiler breeder hens (30-week-old) were fed for 30 days. Egg and body parameters significantly changed between treatments (p&lt;0.05). Effect of different level of feed intake on ovarian morphology parameters was significant (p&lt;0.05), except for white follicles. After 30 days of experiment, plasma nitrite/nitrate (as a index of plasma nitric oxide) and leptin-like hormone increased in FI+20% and FI+40% groups as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler breeder; Follicle; Leptin-like hormone; Nitric oxide; Over/under-fed.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000400004

Effects of Guanidionoacetic Acid and Arginine Supplementation to Vegetable Diets Fed to Broiler Chickens Subjected to Heat Stress before Slaughter
Provedor de dados: 89
Autores: Esser,AFG; Gonçalves,DRM; Rorig,A; Cristo,AB; Perini,R; Fernandes,JIM.
ABSTRACT The objective of this study was to evaluate the supplementation of guanidinoacetic acid (GAA) and L-arginine (L-Arg) as creatine precursors to vegetable diets on the carcass yield and meat quality of broilers subjected to two days of heat stress before slaughter. A total of 1260 broiler chicks were distributed according to a completely randomized design into four treatments with nine replicates of 35 birds each. The treatments consisted of: T1 - vegetable diet based on corn and soybean meal (control diet); T2 - control diet with the inclusion of meat meal (3%); T3 - control diet supplemented with GAA (0.08%); and T4 - control diet supplemented with L-Arg (0.8%). The birds were submitted to heat stress for two days before slaughter (from 42 to 44...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abdominal fat; Creatine; Meat quality; Nitric oxide.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300429

Hydrogen sulfide: a new endogenous player in an old mechanism of plant tolerance to high salinity
Provedor de dados: 39
Autores: da-Silva,Cristiane J.; Modolo,Luzia V.
ABSTRACT High salinity affects plants due to stimulation of osmotic stress. Cell signaling triggered by nitric oxide (NO) and hydrogen sulfide (H2S) activates a cascade of biochemical events that culminate in plant tolerance to abiotic and biotic stresses. For instance, the NO/H2S-stimulated biochemical events that occur in plants during response to high salinity include the control of reactive oxygen species, activation of antioxidant system, accumulation of osmoprotectants in cytosol, induction of K+ uptake and Na+ cell extrusion or its vacuolar compartmentation among others. This review is a compilation of what we have learned in the last 10 years about NO participation during cell signaling in response to high salinity as well as the role of H2S, a new...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic stress; Cell signaling; Hydrogen sulfide; Nitric oxide; NO and H2S biosynthesis; Salt stress.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100150

Arginine metabolism in uricotelic species - doi: 10.4025/actascianimsci.v32i4.10990
Provedor de dados: 2
Autores: Fernandes, Jovanir Inês Müller; Universidade Federal do Paraná; Murakami, Alice Eiko; UEM.
Due to the lack of a complete urea cycle, uricotelic species, such as broilers, are not able to synthesize de novo arginine (Arg), thus depending exclusively on dietary Arg. High levels of dietary lysine (Lys) increase the demand for Arg because of the antagonistic relationship between these amino acids. The Arg-Lys antagonism promotes an expressive increase in the renal Arg activity and consequently induces the degradation of Arg and the decrease in the activity of glycine amidinotransferase, an enzyme that uses Arg in the synthesis of muscle creatin. Arg is considered an important modulator of immunological and physiological processes. The degradation of Arg produces ornithine, a precursor of polyamines that are key to cell division, DNA synthesis, and...
Palavras-chave: Carbamoyl phosphatase synthetase; Creatine; Immune system; Nitric oxide; Polyamines; Urea cycle carbamoyl phosphatase synthetase; Creatine; Immune system; Nitric oxide; Polyamines; Urea cycle.
Ano: 2010
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10990

Sublethal effect of concentrations of Metarhizium anisopliae (metsch.) sorok on the larval stage and immunologic characteristics of Diatraea flavipennella (box) (lepidoptera: crambidae)
Provedor de dados: 42
Autores: GUIMARÃES,JENNIFER; MARQUES,EDMILSON J.; WANDERLEY-TEIXEIRA,VALÉRIA; ALBUQUERQUE,AURISTELA C. DE; PASSOS,ELIANA M. DOS; SILVA,CÍNTHIA C.M.; TEIXEIRA,ÁLVARO A.C.
This work aimed to evaluate the effects of Metarhizium anisopliae on Diatraea flavipennella and investigate their immune response. Was observed the effect of M. anisopliae against larvae of D. flavipennella sprayed at concentrations of 103, 104, 105 conidia / mL, in which showed differences relative the larval period, extending up to 72.0 days in treatment and 25.0 days in the control. The results for hemocytes revealed that the most frequent cells when sprayed at the concentrations of 103, 105, 107 conidia / mL were the prohemocytes, spherulocytes, plasmatocytes and granulocytes in relation to adipohemocytes and oenocytoids. The level of nitric oxide was different between the control and the concentration 107 spores / mL (24), while the activity of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Entomopathogen; Hemocytes; Histochemistry; Nitric oxide; Phenoloxidases; Sugarcane borer.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401973

Inhibitory effects of Zanthoxylum rhoifolium Lam. (Rutaceae) against the infection and infectivity of macrophages by Leishmania amazonensis
Provedor de dados: 42
Autores: MELO NETO,BERNARDO; LEITÃO,JOSEANA M.S.R.; OLIVEIRA,LUCIANO G.C.; SANTOS,SÉRGIO E.M.; CARNEIRO,SABRINA M.P.; RODRIGUES,KLINGER A.F.; CHAVES,MARIANA H.; ARCANJO,DANIEL D.R.; CARVALHO,FERNANDO A.A.
ABSTRACT Zanthoxylum rhoifolium Lam. (Rutaceae) has been traditionally used in the treatment of microbial infections and parasitic diseases. In the present study, the antileishmanial effect induced by the ethanol extract of stem barks from Z. rhoifolium (ZR-EEtOH) and its n-hexane fraction (ZR-FHEX) on infection and infectivity of murine macrophages by promastigote forms of Leishmania amazonensis were investigated. In different set of experiments, macrophages or promastigotes were pretreated with ZR-EEtOH or ZR-FHEX at non-lethal concentrations for 24 hours, and then macrophages were submitted to infection by promastigotes. Moreover, their effects on activation of macrophages, as well as on the DNA content, size and number of promastigotes by flow...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Macrophages; Nitric oxide; Promastigote; Phagocytic activity; Zanthoxylum.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501851

Immunology and immunopathology of African trypanosomiasis
Provedor de dados: 42
Autores: Vincendeau,Philippe; Bouteille,Bernard.
Major modifications of immune system have been observed in African trypanosomiasis. These immune reactions do not lead to protection and are also involved in immunopathology disorders. The major surface component (variable surface glycoprotein,VSG) is associated with escape to immune reactions, cytokine network dysfunctions and autoantibody production. Most of our knowledge result from experimental trypanosomiasis. Innate resistance elements have been characterised. In infected mice, VSG preferentially stimulates a Th 1-cell subset. A response of &lt;FONT FACE=Symbol&gt;gd&lt;/FONT&gt; and CD8 T cells to trypanosome antigens was observed in trypanotolerant cattle. An increase in CD5 B cells, responsible for most serum IgM and production of autoantibodies...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Trypanosome; African trypanosomiasis; Immunology; Macrophage; Lymphocytes; Nitric oxide; Cytokine; Autoantibodies.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400004

ABA regulation of post-germination desiccation tolerance in wheat cultivars contrasting in drought tolerance
Provedor de dados: 42
Autores: KOUR,SATINDER; ZHAWAR,VIKRAMJIT K.
ABSTRACT Post-germination desiccation tolerance (DT) was studied in two wheat cultivars. Effect of pretreatment of abscisic acid (ABA)/ osmotic/ salt/ heat stress was also studied. One day (d)-old seedlings of wheat cultivars PBW 644 (drought tolerant) and PBW 343 (drought susceptible) were exposed to ABA/stress treatment for next 1 d, desiccated for 4 d and subsequently rehydrated for 4 d. Biomass, protein, water, protein carbonyls (oxidative toxicity) and nitric oxide (NO) levels were measured in 2 d (treated), 6 d (desiccated), 10 d (rehydrated) seedlings. Vegetative reactive oxygen species (ROS)/ NO-pathways were studied under normal condition and ABA supply by supplying ROS/NO scavengers. Desiccation caused water loss and increased oxidative toxicity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Abiotic; Abscisic acid; Nitric oxide; Post germination desiccation tolerance; Wheat.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401493

Removal of the cortical projections alters expression of NOS in the different cell types of the superficial layers of the superior colliculus in rats
Provedor de dados: 42
Autores: TENÓRIO,FRANK; GIRALDI-GUIMARÃES,ARTHUR; MENDEZ-OTERO,ROSALIA.
Nitric oxide has several biological roles and nitric oxide synthase (NOS) is expressed in the nervous system, and co-localizes with NADPH-diaphorase. The superficial layers of the superior colliculus (SC), which receive retinal and cortical inputs, present NADPH-d staining in a sub-population of neurons that include all cell types. We have previously shown, by NADPH-diaphorase, that eye enucleation alters the intracellular distribution of NOS. Here, we studied the effect of cortical ablation on NOS expression by neurons in collicular superficial layers. Our results show that cortical ablation alters the proportion of different NOS-positive cell types, but not the intracellular distribution of the enzyme.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Superior colliculus; Nitric oxide; Visual cortex; NADPH-diaphorase.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400008

Effects of rotational culture on morphology, nitric oxide production and cell cycle of endothelial cells
Provedor de dados: 47
Autores: Chaojun,Tang; Wu,Xue; Linqi,Ye; Xiang,Xie; Guixue,Wang.
Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Results showed that covering of stents by endothelial cells was improved by rotational culture. NO production decreased within 24 h in both rotational and static culture groups. In addition, rotational culture significantly increased NO production by 37.9% at 36 h and 28.9% at 48 h compared with static culture. Flow cytometry showed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial cells; Rotational culture; Stents; Nitric oxide.
Ano: 2012
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300001

Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala: Fabricius, 1794
Provedor de dados: 47
Autores: Faraldo,A. C.; Sá-Nunes,A; Faccioli,L. H.; Del Bel,E. A.; Lello,E.
Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: NO synthase; Nitric oxide; NADPH-diaphorase; Chrysomya megacephala; Blowfly; Tissues.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200002

Biochemical-molecular markers in unilateral ureteral obstruction
Provedor de dados: 47
Autores: Manucha,Walter.
Congenital obstructive nephropathy is the primary cause of end-stagerenal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstr uctive uropathy effects-decline in the plasmatic renal flow and glomerular filtrationrate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosisare a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-b (TGF-b) and nitric oxide (NO) have been implicated in congenital obstructive nephropathy. The renin-angiotensin system is regulated in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Unilateral ureteral obstruction; Interstitial fibrosis; Nitric oxide; Angiotensin II; Oxidative stress.
Ano: 2007
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100001

Nitric oxide and carotid body chemoreception
Provedor de dados: 48
Autores: ITURRIAGA,RODRIGO.
Nitric oxide (NO) has been proposed as an inhibitory modulator of carotid body chemosensory responses to hypoxia. It is believed that NO modulates carotid chemoreception by several mechanisms, which include the control of carotid body vascular tone and oxygen delivery and reduction of the excitability of chemoreceptor cells and petrosal sensory neurons. In addition to the well-known inhibitory effect, we found that NO has a dual (dose-dependent) effect on carotid chemoreception depending on the oxygen pressure level. During hypoxia, NO is primarily an inhibitory modulator of carotid chemoreception, while in normoxia NO increased the chemosensory activity. This excitatory effect produced by NO is likely mediated by an impairment of mitochondrial electron...
Tipo: Journal article
Palavras-chave: Carotid body; Chemoreceptor; Cytochrome oxidase; Hypoxia; Nitric oxide; Oxidative phosphorylation.
Ano: 2001
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200019

Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
Provedor de dados: 48
Autores: GARCÍA,KATHERINE P; RUBILAR,PAULINA S; VARGAS,MACARENA F; CÁRDENAS,HUGO; RIOS,MIGUEL A; ORIHUELA,PEDRO A; VARGAS,RENATO H ; FUHRER,JUAN; HECKELS,JOHN E; CHRISTODOULIDES,MYRON; VELÁSQUEZ,LUIS A.
In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release,...
Tipo: Journal article
Palavras-chave: Cellular damage; Fallopian tubes; Neisseria gonorrhoeae; Nitric oxide.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006

Nitric Oxide: Oxygen Radical Interactions in Atherosclerosis
Provedor de dados: 48
Autores: RUBBO,HOMERO; BATTHYANY,CARLOS; RADI,RAFAEL.
Atherosclerosis is one of the most common diseases and the principal cause of death in western civilization. The pathogenesis of this disease can be explained on the basis of the ‘oxidative-modification hypothesis,’ which proposes that low-density lipoprotein (LDL) oxidation represents a key early event. Nitric oxide (.NO) regulates critical lipid membrane and lipoprotein oxidation events by a) contributing to the formation of more potent secondary oxidants from superoxide (i.e.: peroxynitrite), and b) its antioxidant properties through termination reactions with lipid radicals to possibly less reactive secondary nitrogen-containing products (LONO, LOONO). Relative rates of production and steady state concentrations of superoxide and .NO and cellular sites...
Tipo: Journal article
Palavras-chave: Antioxidants; Free radicals; Lipid oxidation; Low density lipoprotein oxidation; Nitric oxide; Peroxynitrite.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200017

Free radical chemistry in biological systems
Provedor de dados: 48
Autores: VALDEZ,LAURA B; LORES ARNAIZ,SILVIA; BUSTAMANTE,JUANITA; ALVAREZ,SILVIA; COSTA,LIDIA E; BOVERIS,ALBERTO.
Mitochondria are an active source of the free radical superoxide (O2-) and nitric oxide (NO), whose production accounts for about 2% and 0.5% respectively, of mitochondrial O2 uptake under physiological conditions. Superoxide is produced by the auto-oxidation of the semiquinones of ubiquinol and the NADH dehydrogenase flavin and NO by the enzymatic action of the nitric oxide synthase of the inner mitochondrial membrane (mtNOS). Nitric oxide reversibly inhibits cytochrome oxidase activity in competition with O2. The balance between NO production and its utilization results in a NO intramitochondrial steady-state concentration of 20-50 nM, which regulates mitochondrial O2 uptake and energy supply. The regulation of cellular respiration and energy production...
Tipo: Journal article
Palavras-chave: Superoxide radical; Nitric oxide; Peroxynitrite; Mitochondria; Apoptosis; Aging.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200005

Gastroprotective effect of the ethanolic extract of Parkia platycephala Benth. leaves against acute gastric lesion models in rodents
Provedor de dados: 48
Autores: Fernandes,Hélio B; Silva,Francilene V; Passos,Flávia Franceli B; Bezerra,Roosevelt D S; Chaves,Mariana H; Oliveira,Francisco A; Meneses Oliveira,Rita C.
Parkia platycephala Benth. (Leguminosae - Mimosoideae), popularly known as "visgueira", fava bean tree or "fava-de-bolota", is widely found in the Northern and Northeastern regions of Brazil. Its pods are used as cattle food supplement in the drought period. Compounds with a gastroprotective activity were obtained from the genus Parkia. Therefore, this study aimed at investigating the gastroprotective effect of the ethanolic extract of Parkia platycephala Benth. leaves (Pp-EtOH), as well as evaluating its possible mechanisms of action in experimental ulcer induction models. Lesions were induced by absolute ethanol, ethanol-HCl, ischemia-reperfusion and indomethacin in rodents. Pp-EtOH showed a protective effect in the lesion models (66, 48 and 52 %,...
Tipo: Journal article
Palavras-chave: Antioxidant; Ethanol; Gastroprotection; Nitric oxide; Parkia platycephala; Ulcer.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400010

Diet and Endothelial Function
Provedor de dados: 48
Autores: CUEVAS,ADA M; GERMAIN,ALFREDO M.
Endothelial dysfunction is one of the earliest events in atherogenesis. A consequence of endothelial damage is a lower availability of nitric oxide (NO), the most potent endogenous vasodilator. NO inhibits platelet aggregation, smooth muscle cell proliferation and adhesion of monocytes to endothelial cells. Endothelial dysfunction is present in patients with cardiovascular disease and/or coronary risk factors, such as hypertension, dyslipidemia, diabetes, smoking or hyperhomocysteinemia. At present, soluble markers and high resolution ultrasound of the brachial artery, have provided simple tools for the study of endothelial function and the effects of several interventions. It has been demonstrated that dietary factors may induce significant changes on...
Tipo: Journal article
Palavras-chave: Diet; Endothelial function; Nitric oxide; Vascular reactivity.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200008

Regulation of capacitative and non-capacitative Ca2+ entry in A7r5 vascular smooth muscle cells
Provedor de dados: 48
Autores: TAYLOR,COLIN W; MONEER,ZAHID.
A capacitative Ca2+ entry (CCE) pathway, activated by depletion of intracellular Ca2+ stores, is thought to mediate much of the Ca2+ entry evoked by receptors that stimulate phospholipase C (PLC). However, in A7r5 vascular smooth muscle cells, vasopressin, which stimulates PLC, empties intracellular Ca2+ stores but simultaneously inhibits their ability to activate CCE. The diacylglycerol produced with the IP3 that empties the stores is metabolized to arachidonic and this leads to activation of nitric oxide (NO) synthase, production of NO and cyclic GMP, and consequent activation of protein kinase G. The latter inhibits CCE. In parallel, NO directly activates a non-capacitative Ca2+ entry (NCCE) pathway, which is entirely responsible for the Ca2+ entry that...
Tipo: Journal article
Palavras-chave: Ca2+ entry; Smooth muscle; Archidonic acid; Nitric oxide; Cyclic GMP.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400020

Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
Provedor de dados: 48
Autores: LANZA,HENRIQUE; AFONSO-CARDOSO,SANDRA R; SILVA,ADRIANO G; NAPOLITANO,DANIELLE R; ESPÍNDOLA,FOUED S; PENA,JANETHE D.O; SOUZA,MARIA A.
Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine...
Tipo: Journal article
Palavras-chave: Calcium; Leishmania major; Macrophage; Magnesium; Nitric oxide.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004

Effect of exogenous nitric oxide on sperm motility in vitro
Provedor de dados: 48
Autores: Wang,Jiangtao; He,Qingliu; Yan,Xingyu; Cai,Youmei; Chen,Junyi.
BACKGROUND: Nitric oxide (NO) has been shown to be important in sperm function, and the concentration of NO appears to determine these effects. Studies have demonstrated both positive and negative effects of NO on sperm function, but have not been able to provide a clear link between NO concentration and the extent of exposure to NO. To study the relationship between nitric oxide and sperm capacitationin vitro, and to provide a theoretical basis for the use of NO-related preparations in improving sperm motility for in vitro fertilization, we investigated the effects of NO concentration and time duration at these concentrations on in vitro sperm capacitation in both normal and abnormal sperm groups. We manipulated NO concentrations and the time duration of...
Tipo: Journal article
Palavras-chave: Nitric oxide; Sperm capacitation; Sperm motility; Time; Human.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100044

Effect of nitric oxide and plant antioxidants on microsomal content of lipid radicals
Provedor de dados: 48
Autores: BOVERIS,ALEJANDRO D; GALATRO,ANDREA; PUNTARULO,SUSANA.
The antioxidant ability of nitric oxide (NO) generated by a chemical donor and of commercially available antioxidant preparations was assayed. SNAP (S-Nitroso-N-acetylpenicilamine) was used as the NO donor, and Ginkgo biloba, wheat and alfalfa preparations were tested. Lipid peroxidation was assayed by EPR employing a reaction system consisting of rat liver microsomes, ADP, FeCl3, NADPH and POBN in phosphate buffer, pH=7.4. In vitro NO exposure decreased microsomal lipid peroxidation in a dose-dependent manner. The dose responsible for inhibiting the microsomal content of lipid radical adducts by 50% (LD50) for SNAP was 550 mu M (NO generation rate 0.1 mu M/min). The addition of 50 mM hemoglobin to the incubation media prevented NO effect on lipid...
Tipo: Journal article
Palavras-chave: Antioxidants; Nitric oxide; Plants extracts; Lipid radicals.
Ano: 2000
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200016

Cytotoxicity, nitric oxide and acetylcholinesterase inhibitory activity of three limonoids isolated from Trichilia welwitschii (Meliaceae)
Provedor de dados: 48
Autores: Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N.
BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (&amp;#956;g/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin...
Tipo: Journal article
Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057

The effect of phosphoethanolamine intake on mortality and macrophage activity in mice with solid ehrlich tumors
Provedor de dados: 52
Autores: Arruda,Maria Sueli Parreira de; Correa,Mara Adriana; Venturini,James; Félix,Maira Camila; Rosis,Ana Maria Bormio De; Galhiane,Mário Sérgio; Chierice,Gilberto Orivaldo; Costa,Maria Renata Sales Nogueira.
The aim of the present study was to examine the effect of a diet rich in synthetic PEtn on the metabolism macrophages of tumor-bearing mice. The results demonstrated that PEtn increased the animals' survival time. In addition, the treated animals released smaller amounts of hydrogen peroxide (H2O2) and nitric oxide (NO) than the non-treated animals, particularly after day 14. From the results it could be concluded that H2O2 and NO were important in the modulation of neoplastic growth, and pointed to a promising role of PEtn in the control of human neoplasms.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phosphoethanolamine; Ehrlich tumor; Nitric oxide; Hydrogen peroxide; Lipids.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600016

The effects of nitric oxide on the immune response during giardiasis
Provedor de dados: 55
Autores: Pavanelli,Wander Rogério; Gutierrez,Fredy Roberto Salazar; Silva,Jean Jerley Nogueira da; Costa,Ivete Conchon; Menezes,Maria Claudia Noronha Dutra de; Oliveira,Francisco José de Abreu; Itano,Eiko Nakagawa; Watanabe,Maria Angélica Ehara.
Nitric oxide (NO) is a free radical synthesized from L-arginine by different isoforms NO-synthases. NO possesses multiple and complex biological functions. NO is an important mediator of homeostasis, and changes in its generation or actions can contribute or not to pathological states. The knowledge of effects of NO has been not only important to our understanding of immune response, but also to new tools for research and treatment of various diseases. Knowing the importance of NO as inflammatory mediator in diverse infectious diseases, we decided to develop a revision that shows the participation/effect of this mediator in immune response induced against Giardia spp. Several studies already demonstrated the participation of NO with microbicidal and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Immune system; Giardiasis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600010

Involvement of nitric oxide and its up/down stream molecules in the immunity against parasitic infections
Provedor de dados: 55
Autores: Nahrevanian,Hossein.
Nitric oxide (NO) is a potent mediator with diverse roles in regulating cellular functions and signaling pathways. The NO synthase (NOS) enzyme family consists of three major isoforms, which convey variety of messages between cells, including signals for vasorelaxation, neurotransmission and cytotoxicity. This family of enzymes are generally classified as neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS). Increased levels of NO are induced from iNOS during infection; while eNOS and nNOS may be produced at the baseline in normal conditions. An association of some key cytokines appears to be essential for NOS gene regulation in the immunity of infections. Accumulating evidence indicates that parasitic diseases are commonly associated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: INOS; Nitric oxide; NO; Immunity; Helminth; Parasite; Protozoa.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600010

Immune effector mechanisms of the nitric oxide pathway in malaria: cytotoxicity versus cytoprotection
Provedor de dados: 55
Autores: Nahrevanian,Hossein.
Nitric oxide (NO) is thought to be an important mediator and critical signaling molecule for malaria immunopathology; it is also a target for therapy and for vaccine. Inducible nitric oxide synthase (iNOS) is synthesized by a number of cell types under inflammatory conditions. The most relevant known triggers for its expression are endotoxins and cytokines. To date, there have been conflicting reports concerning the clinical significance of NO in malaria. Some researchers have proposed that NO contributes to the development of severe and complicated malaria, while others have argued that NO has a protective role. Infection with parasites resistant to the microbicidal action of NO may result in high levels of NO being generated, which could then damage the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: INOS; Malaria; Nitric oxide; NO; RNI.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400014

Effects of nitric oxide on magnocellular neurons of the supraoptic nucleus involve multiple mechanisms
Provedor de dados: 56
Autores: Silva,M.P. da; Cedraz-Mercez,P.L.; Varanda,W.A.
Physiological evidence indicates that the supraoptic nucleus (SON) is an important region for integrating information related to homeostasis of body fluids. Located bilaterally to the optic chiasm, this nucleus is composed of magnocellular neurosecretory cells (MNCs) responsible for the synthesis and release of vasopressin and oxytocin to the neurohypophysis. At the cellular level, the control of vasopressin and oxytocin release is directly linked to the firing frequency of MNCs. In general, we can say that the excitability of these cells can be controlled via two distinct mechanisms: 1) the intrinsic membrane properties of the MNCs themselves and 2) synaptic input from circumventricular organs that contain osmosensitive neurons. It has also been...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Magnocellular neurons; Supraoptic nucleus; Vasopressin; Oxytocin; Nitric oxide; Homeostasis.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000200090

Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells
Provedor de dados: 56
Autores: Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.
Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010

Effects of acid-base imbalance on vascular reactivity
Provedor de dados: 56
Autores: Celotto,A.C.; Capellini,V.K.; Baldo,C.F.; Dalio,M.B.; Rodrigues,A.J.; Evora,P.R.B.
Acid-base homeostasis maintains systemic arterial pH within a narrow range. Whereas the normal range of pH for clinical laboratories is 7.35-7.45, in vivo pH is maintained within a much narrower range. In clinical and experimental settings, blood pH can vary in response to respiratory or renal impairment. This altered pH promotes changes in vascular smooth muscle tone with impact on circulation and blood pressure control. Changes in pH can be divided into those occurring in the extracellular space (pHo) and those occurring within the intracellular space (pHi), although, extracellular and intracellular compartments influence each other. Consistent with the multiple events involved in the changes in tone produced by altered pHo, including type of vascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid-base balance; Nitric oxide; Vascular reactivity; Endothelium; Alkalosis; Acidosis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000600001

Preeclampsia: from epidemiological observations to molecular mechanisms
Provedor de dados: 56
Autores: López-Jaramillo,P.; Casas,J.P.; Serrano,N.
Preeclampsia is the main cause of maternal mortality and is associated with a five-fold increase in perinatal mortality in developing countries. In spite of this, the etiology of preeclampsia is unknown. The present article analyzes the contradictory results of the use of calcium supplementation in the prevention of preeclampsia, and tries to give an explanation of these results. The proposal of an integrative model to explain the clinical manifestations of preeclampsia is discussed. In this proposal we suggest that preeclampsia is caused by nutritional, environmental and genetic factors that lead to the creation of an imbalance between the free radicals nitric oxide, superoxide and peroxynitrate in the vascular endothelium. The adequate interpretation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Preeclampsia; Calcium supplementation; Nitric oxide; Infection; Inflammation; Oxidative stress; Endothelial dysfunction.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000001

Activation of neural cholecystokinin-1 receptors induces relaxation of the isolated rat duodenum which is reduced by nitric oxide synthase inhibitors
Provedor de dados: 56
Autores: Martins,S.R.; de Oliveira,R.B.; Ballejo,G.
Cholecystokinin (CCK) influences gastrointestinal motility, by acting on central and peripheral receptors. The aim of the present study was to determine whether CCK has any effect on isolated duodenum longitudinal muscle activity and to characterize the mechanisms involved. Isolated segments of the rat proximal duodenum were mounted for the recording of isometric contractions of longitudinal muscle in the presence of atropine and guanethidine. CCK-8S (EC50: 39; 95% CI: 4.1-152 nM) and cerulein (EC50: 58; 95% CI: 18-281 nM) induced a concentration-dependent and tetrodotoxin-sensitive relaxation. Nomeganitro-L-arginine (L-NOARG) reduced CCK-8S- and cerulein-induced relaxation (IC50: 5.2; 95% CI: 2.5-18 µM) in a concentration-dependent manner. The magnitude...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CCK; CCK receptor subtype 1; Proximal duodenum; Myenteric neurons; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200014

Cytokine profile and nitric oxide levels in sera from patients with brucellosis
Provedor de dados: 56
Autores: Refik,M.; Mehmet,N.; Durmaz,R.; Ersoy,Y.
The aims of this study were to investigate the serum levels of some cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1ß (IL-1ß), IL-2R, IL-6, and IL-8] and nitric oxide (NO) levels in patients with untreated brucellosis and to test the correlation of these parameters with each other. The study was conducted on 67 subjects, 37 patients with brucellosis and 30 healthy individuals with no history of Brucella infection. Brucellosis was identified by a positive blood culture and/or increased Brucella antibodies in serological tests in addition to compatible clinical symptoms. Cytokine profile analysis was performed by the immulite chemiluminescent enzyme immunometric assay whose inter- and intra-assay coefficients of variance were 2.6-3.6 and...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Human brucellosis; Cytokines; Nitric oxide; Interleukin-2; Interleukin-6; Interleukin-8.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100010

Fine-tuning of defensive behaviors in the dorsal periaqueductal gray by atypical neurotransmitters
Provedor de dados: 56
Autores: Fogaça,M.V.; Lisboa,S.F.; Aguiar,D.C.; Moreira,F.A.; Gomes,F.V.; Casarotto,P.C.; Guimarães,F.S.
This paper presents an up-to-date review of the evidence indicating that atypical neurotransmitters such as nitric oxide (NO) and endocannabinoids (eCBs) play an important role in the regulation of aversive responses in the periaqueductal gray (PAG). Among the results supporting this role, several studies have shown that inhibitors of neuronal NO synthase or cannabinoid receptor type 1 (CB1) receptor agonists cause clear anxiolytic responses when injected into this region. The nitrergic and eCB systems can regulate the activity of classical neurotransmitters such as glutamate and γ-aminobutyric acid (GABA) that control PAG activity. We propose that they exert a ‘fine-tuning’ regulatory control of defensive responses in this area. This control, however, is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocannabinoid; Anandamide; Nitric oxide; Endovanilloid; TRPV1.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400010

Endothelium-dependent and -independent hepatic artery vasodilatation is not impaired in a canine model of liver ischemia-reperfusion injury
Provedor de dados: 56
Autores: Miranda,L.C.; Viaro,F.; Ceneviva,R.; Evora,P.R.B.
We investigated whether hepatic artery endothelium may be the earliest site of injury consequent to liver ischemia and reperfusion. Twenty-four heartworm-free mongrel dogs of either sex exposed to liver ischemia/reperfusion in vivo were randomized into four experimental groups (N = 6): a) control, sham-operated dogs, b) dogs subjected to 60 min of ischemia, c) dogs subjected to 30 min of ischemia and 60 min of reperfusion, and d) animals subjected to 45 min of ischemia and 120 min of reperfusion. The nitric oxide endothelium-dependent relaxation of hepatic artery rings contracted with prostaglandin F2a and exposed to increasing concentrations of acetylcholine, calcium ionophore A23187, sodium fluoride, phospholipase-C, poly-L-arginine, isoproterenol, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium; Hepatic artery; Nitric oxide; Liver; Ischemia/reperfusion injury.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000600016

Hypotrophy of conduit artery walls of the offspring of nitric oxide-defective rats
Provedor de dados: 56
Autores: Kristek,F.; Gerová,M.
The objective of the present study was to investigate the structure of the arterial walls of the offspring stemming from nitric oxide (NO)-defective hypertensive parents. The parents were treated with N G-nitro-L-arginine methyl ester (40 mg kg-1 day-1) for 5 weeks. Blood pressure was measured noninvasively in six 30-day-old rats and nine age-matched controls. The cardiovascular system was perfused with glutaraldehyde at 120 mmHg. The thoracic aorta and carotid artery were processed for electron microscopy, and geometry was determined by light microscopy. Endothelial cells, smooth muscle cells (SMC) and extracellular matrix (ECM) were determined by the point counting method in electron micrographs of the carotid artery. The blood pressure of experimental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Artery; Nitric oxide; Offspring; Hypertension; Vascular smooth muscle; Extracellular matrix; Morphometry.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400018

Effect of injection of L-NAME on drinking response
Provedor de dados: 56
Autores: Saad,W.A.; Camargo,L.A.A.; Saad,R.; Pereira,A.F.; Simões,S.
The drinking behavior responses to centrally administered NG-nitro-L-arginine methyl ester (L-NAME; 10, 20 or 40 µg/µl), an inhibitor of nitric oxide synthase, were studied in satiated rats, with cannulae stereotaxically implanted into the lateral ventricle (LV) and subfornical organ (SFO). Water intake increased in all animals after angiotensin II (ANG II) injection into the LV, with values of 14.2 ± 1.4 ml/h. After injection of L-NAME at doses of 10, 20 or 40 µg/µl into the SFO before injection of ANG II (12 ng/µl) into the LV, water intake decreased progressively and reached basal levels after treatment with 0.15 M NaCl and with the highest dose of L-NAME (i.e., 40 µg). The water intake obtained after 40 µg/µl L-NAME was 0.8 ± 0.01 ml/h. Also, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Water intake; SFO; Dehydration; CNS.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100012

Evidence for the participation of nitric oxide in pemphigus
Provedor de dados: 56
Autores: Siebra,M.X.; Santos,M.A.; Almeida,T.L.P.; Leite,A.C.R.M.; Cunha,F.Q.; Rocha,F.A.C.
Pemphigus is an inflammatory autoimmune disorder of the skin. Nitric oxide (NO) is an inflammatory mediator linked to a variety of physiological and pathophysiological phenomena that include skin tumors, psoriasis, urticaria, and atopic dermatitis. Inflammatory cells present in pemphigus lesions are important sources of NO production. We investigated whether NO is involved in pemphigus. A prospective cohort study was conducted at the Dermatology Service of the Hospital Universitário Walter Cantídio of the Federal University of Ceará. All patients seen at the outpatient clinic between August 2000 and July 2001, with a clinically and histologically confirmed diagnosis of pemphigus were included. The median age was 42.5 years (range: 12-69 years) with a male...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Autoimmunity; Inflammation; Neutrophils; Nitric oxide; Pemphigus; Inducible nitric oxide synthase.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500014

Oral administration of L-arginine decreases blood pressure and increases renal excretion of sodium and water in renovascular hypertensive rats
Provedor de dados: 56
Autores: Gouvêa,S.A.; Moysés,M.R.; Bissoli,N.S.; Pires,J.G.P.; Cabral,A.M.; Abreu,G.R.
The two-kidney, one-clip renovascular (2K1C) hypertension model is characterized by a reduction in renal flow on the clipped artery that activates the renin-angiotensin system. Endothelium dysfunction, including diminished nitric oxide production, is also believed to play a role in the pathophysiology of this model. Some studies have shown an effect of L-arginine (L-Arg, a nitric oxide precursor) on hypertension. In the present study we determined the ability of L-Arg (7 days of treatment) to reduce blood pressure and alter renal excretions of water, Na+ and K+ in a model of 2K1C-induced hypertension. Under ether anesthesia, male Wistar rats (150-170 g) had a silver clip (0.20 mm) placed around the left renal artery to produce the 2K1C renovascular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: L-arginine; Nitric oxide; Renovascular hypertension; Sodium excretion; Renal physiology; Blood pressure.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700017

5-HT1A autoreceptor modulation of locomotor activity induced by nitric oxide in the rat dorsal raphe nucleus
Provedor de dados: 56
Autores: Gualda,L.B.; Martins,G.G.; Müller,B.; Guimarães,F.S.; Oliveira,R.M.W.
The dorsal raphe nucleus (DRN) is the origin of ascending serotonergic projections and is considered to be an important component of the brain circuit that mediates anxiety- and depression-related behaviors. A large fraction of DRN serotonin-positive neurons contain nitric oxide (NO). Disruption of NO-mediated neurotransmission in the DRN by NO synthase inhibitors produces anxiolytic- and antidepressant-like effects in rats and also induces nonspecific interference with locomotor activity. We investigated the involvement of the 5-HT1A autoreceptor in the locomotor effects induced by NO in the DRN of male Wistar rats (280-310 g, N = 9-10 per group). The NO donor 3-morpholinosylnomine hydrochloride (SIN-1, 150, and 300 nmol) and the NO scavenger...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Dorsal raphe nucleus; Nitric oxide; Serotonin; 5-HT1A receptor; Locomotor activity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400010

Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood
Provedor de dados: 56
Autores: Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R.
Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007

Effects of a neuronal nitric oxide synthase inhibitor on lipopolysaccharide-induced fever
Provedor de dados: 56
Autores: Perotti,C.A.A.; Nogueira,M.S.; Antunes-Rodrigues,J.; Cárnio,E.C.
It has been demonstrated that nitric oxide (NO) has a thermoregulatory action, but very little is known about the mechanisms involved. In the present study we determined the effect of neuronal nitric oxide synthase (nNOS) inhibition on thermoregulation. We used 7-nitroindazole (7-NI, 1, 10 and 30 mg/kg body weight), a selective nNOS inhibitor, injected intraperitoneally into normothermic Wistar rats (200-250 g) and rats with fever induced by lipopolysaccharide (LPS) (100 µg/kg body weight) administration. It has been demonstrated that the effects of 30 mg/kg of 7-NI given intraperitoneally may inhibit 60% of nNOS activity in rats. In all experiments the colonic temperature of awake unrestrained rats was measured over a period of 5 h at 15-min intervals...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; 7-nitroindazole; Body temperature; LPS; L-NAME.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100008

Sex differences in angiotensin II- induced hypertension
Provedor de dados: 56
Autores: Xue,B.; Johnson,A.K.; Hay,M.
Sex differences in the development of hypertension and cardiovascular disease have been described in humans and in animal models. In this paper we will review some of our studies which have as their emphasis the examination of the role of sex differences and sex steroids in modulating the central actions of angiotensin II (ANG II) via interactions with free radicals and nitric oxide, generating pathways within brain circumventricular organs and in central sympathomodulatory systems. Our studies indicate that low-dose infusions of ANG II result in hypertension in wild-type male mice but not in intact wild-type females. Furthermore, we have demonstrated that ANG II-induced hypertension in males is blocked by central infusions of the androgen receptor...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Angiotensin II; Estrogen; Estrogen a and ß receptors; Nitric oxide; Reactive oxygen species; Testosterone.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500018

Neuronal and endothelial nitric oxide synthase gene knockout mice
Provedor de dados: 56
Autores: Huang,P.L.
Targeted disruption of the neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS) genes has led to knockout mice that lack these isoforms. These animal models have been useful to study the roles of nitric oxide (NO) in physiologic processes. nNOS knockout mice have enlarged stomachs and defects in the inhibitory junction potential involved in gastrointestinal motility. eNOS knockout mice are hypertensive and lack endothelium-derived relaxing factor activity. When these animals are subjected to models of focal ischemia, the nNOS mutant mice develop smaller infarcts, consistent with a role for nNOS in neurotoxicity following cerebral ischemia. In contrast, eNOS mutant mice develop larger infarcts, and show a more pronounced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Targeted disruption; Gene knockout; Endothelium; Stroke; Atherosclerosis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100005

Central release of nitric oxide mediates antinociception induced by aerobic exercise
Provedor de dados: 56
Autores: Galdino,G.S.; Duarte,I.D.; Perez,A.C.
Nitric oxide (NO) is a soluble gas that participates in important functions of the central nervous system, such as cognitive function, maintenance of synaptic plasticity for the control of sleep, appetite, body temperature, neurosecretion, and antinociception. Furthermore, during exercise large amounts of NO are released that contribute to maintaining body homeostasis. Besides NO production, physical exercise has been shown to induce antinociception. Thus, the present study aimed to investigate the central involvement of NO in exercise-induced antinociception. In both mechanical and thermal nociceptive tests, central [intrathecal (it) and intracerebroventricular (icv)] pretreatment with inhibitors of the NO/cGMP/KATP pathway (L-NOArg, ODQ, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Exercise; Pain; Antinociception.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900790

Methylmercury inhibits prolactin release in a cell line of pituitary origin
Provedor de dados: 56
Autores: Maués,L.A.L.; Macchi,B.M.; Crespo-López,M.E.; Nasciutti,L.E.; Picanço-Diniz,D.L.W.; Antunes-Rodrigues,J.; Nascimento,J.L.M. do.
Heavy metals, such as methylmercury, are key environmental pollutants that easily reach human beings by bioaccumulation through the food chain. Several reports have demonstrated that endocrine organs, and especially the pituitary gland, are potential targets for mercury accumulation; however, the effects on the regulation of hormonal release are unclear. It has been suggested that serum prolactin could represent a biomarker of heavy metal exposure. The aim of this study was to evaluate the effect of methylmercury on prolactin release and the role of the nitrergic system using prolactin secretory cells (the mammosomatotroph cell line, GH3B6). Exposure to methylmercury (0-100 μM) was cytotoxic in a time- and concentration-dependent manner, with an LC50...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mercury; Methylmercury; Prolactin; Oxidative stress; Pituitary; Nitric oxide.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000800691

Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis
Provedor de dados: 56
Autores: Rotta,J.C.G.; Lunardi,C.N.; Tedesco,A.C.
The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light (lambda = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS· and NacyS·) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: S-nitrosothiols; Nitric oxide; Zinc phthalocyanine; Liposomes; Photodynamic therapy.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005

Bi-directional actions of estrogen on the renin-angiotensin system
Provedor de dados: 56
Autores: Brosnihan,K.B.; Senanayake,P.S.; Li,P.; Ferrario,C.M.
Estrogen stimulates the renin-angiotensin system by augmenting both tissue and circulating levels of angiotensinogen and renin. We show, however, that angiotensin converting enzyme (ACE) activity in the circulation and in tissues is reduced in two animal models of postmenopausal chronic hormone replacement. We observed a reduction of ACE activity in association with a significant increase in plasma angiotensin I (Ang I) and hyperreninemia in ovariectomized monkeys treated with Premarin (conjugated equine estrogen) replacement for 30 months. Plasma angiotensin II (Ang II) levels were not increased in monkeys treated with estrogen, suggesting that the decrease in ACE curtailed the formation of the peptide. The Ang II/Ang I ratio, an in vivo index of ACE...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Renin; Angiotensin converting enzyme; Angiotensin peptides; Angiotensin receptors; Bradykinin; Nitric oxide; Kinins; Vasodilation; Hormone replacement.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400001

Nitrergic modulation of vasopressin, oxytocin and atrial natriuretic peptide secretion in response to sodium intake and hypertonic blood volume expansion
Provedor de dados: 56
Autores: Ventura,R.R.; Gomes,D.A.; Reis,W.L.; Elias,L.L.K.; Castro,M.; Valença,M.M.; Carnio,E.C.; Rettori,V.; McCann,S.M.; Antunes-Rodrigues,J.
The central nervous system plays an important role in the control of renal sodium excretion. We present here a brief review of physiologic regulation of hydromineral balance and discuss recent results from our laboratory that focus on the participation of nitrergic, vasopressinergic, and oxytocinergic systems in the regulation of water and sodium excretion under different salt intake and hypertonic blood volume expansion (BVE) conditions. High sodium intake induced a significant increase in nitric oxide synthase (NOS) activity in the medial basal hypothalamus and neural lobe, while a low sodium diet decreased NOS activity in the neural lobe, suggesting that central NOS is involved in the control of sodium balance. An increase in plasma concentrations in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasopressin; Oxytocin; ANP; Nitric oxide; Blood volume expansion; Sodium diet.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000900011

Pancreatic nitric oxide and oxygen free radicals in the early stages of streptozotocin-induced diabetes mellitus in the rat
Provedor de dados: 56
Autores: González,E.; Roselló-Catafau,J.; Jawerbaum,A.; Sinner,D.; Pustovrh,C.; Vela,J.; White,V.; Xaus,C.; Peralta,C.; Gimeno,M.
The objective of the present study was to explore the regulatory mechanisms of free radicals during streptozotocin (STZ)-induced pancreatic damage, which may involve nitric oxide (NO) production as a modulator of cellular oxidative stress. Removal of oxygen species by incubating pancreatic tissues in the presence of polyethylene glycol-conjugated superoxide dismutase (PEG-SOD) (1 U/ml) produced a decrease in nitrite levels (42%) and NO synthase (NOS) activity (50%) in diabetic but not in control samples. When NO production was blocked by N G-monomethyl-L-arginine (L-NMMA) (600 µM), SOD activity increased (15.21 ± 1.23 vs 24.40 ± 2.01 U/mg dry weight). The increase was abolished when the NO donor, spermine nonoate, was added to the incubating medium (13.2 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptozotocin diabetes; Pancreas; Oxidative stress; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100012

Antigenic stimulation is more efficient than LPS in inducing nitric oxide production by human mononuclear cells on the in vitro granuloma reaction in schistosomiasis
Provedor de dados: 56
Autores: Oliveira,D.M.; Silva-Teixeira,D.N.; Araújo-Filho,R.; Goes,A.M.
Nitric oxide (NO) is an extremely important and versatile messenger in biological systems. It has been identified as a cytotoxic factor in the immune system, presenting anti- or pro-inflammatory properties under different circumstances. In murine monocytes and macrophages, stimuli by cytokines or lipopolysaccharide (LPS) are necessary for inducing the immunologic isoform of the enzyme responsible for the high-output production of NO, nitric oxide synthase (iNOS). With respect to human cells, however, LPS seems not to stimulate NO production in the same way. Addressing this issue, we demonstrate here that peripheral blood mononuclear cells (PBMC) obtained from schistosomiasis-infected patients and cultivated with parasite antigens in the in vitro granuloma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; LPS; Schistosomiasis infection; In vitro granuloma.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100015

The role of IL-12 in experimental Trypanosoma cruzi infection
Provedor de dados: 56
Autores: Silva,J.S.; Aliberti,J.C.S.; Martins,G.A.; Souza,M.A.; Souto,J.T.; Pádua,M.A.
Host resistance to Trypanosoma cruzi infection is dependent on both natural and acquired immune responses. During the early acute phase of infection in mice, natural killer (NK) cell-derived IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; is involved in controlling intracellular parasite replication, mainly through the induction of nitric oxide biosynthesis by activated macrophages. We have shown that IL-12, a powerful inducer of IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; production by NK cells, is synthesized soon after trypomastigote-macrophage interaction. The role of IL-12 in the control of T. cruzi infection in vivo was determined by treating infected mice with anti-IL-12 monoclonal antibody (mAb) and analyzing both parasitemia and mortality during the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IL-12 Trypanosoma cruzi NK cells IFN-&lt; FONT FACE=Symbol&gt; G&lt; /font&gt; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100014

Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects
Provedor de dados: 56
Autores: Mariano,F.S.; Campanelli,A.P.; Nociti Jr.,F.H.; Mattos-Graner,R.O.; Gonçalves,R.B.
Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antimicrobial peptides; Nitric oxide; Neutrophils; Periodontitis; Innate immunity.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004

Cellular signaling with nitric oxide and cyclic GMP
Provedor de dados: 56
Autores: Murad,F.
During the past two decades, nitric oxide signaling has been one of the most rapidly growing areas in biology. This simple free radical gas can regulate an ever growing list of biological processes. In most instances nitric oxide mediates its biological effects by activating guanylyl cyclase and increasing cyclic GMP synthesis. However, the identification of effects of nitric oxide that are independent of cyclic GMP is also growing at a rapid rate. The effects of nitric oxide can mediate important physiological regulatory events in cell regulation, cell-cell communication and signaling. Nitric oxide can function as an intracellular messenger, neurotransmitter and hormone. However, as with any messenger molecule, there can be too much or too little of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Guanylyl cyclase; Cell signaling.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100001

Antagonic effect of the inhibition of inducible nitric oxide on the mortality of mice acutely infected with Escherichia coli and Bacteroides fragilis
Provedor de dados: 56
Autores: Astolfi,R.S.; Khouri,D.G.; Brandizzi,L.I.V.; Ávila-Campos,M.J.; Andrade Jr.,H.F. de.
Sepsis, the leading cause of death in intensive care units, is associated with overproduction of nitric oxide (NO) due to inducible NO synthase (iNOS), responsible for some of the pathologic changes. Aminoguanidine (AG) is a selective iNOS inhibitor with reported inconsistent actions in sepsis. To investigate the influence of iNOS, we studied models of acute bacterial sepsis using acute challenges with aerobic (Escherichia coli) and anaerobic (Bacteroides fragilis) bacteria in the presence of AG. Six-week-old, 23 g, male and female BALB/c and C57Bl/6j mice, in equal proportions, were inoculated (ip) with bacteria in groups of 4 animals for each dose and each experiment in the absence or presence of AG (50 mg/kg, ip, starting 24 h before challenge and daily...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Sepsis; Experimental model; Bacterial infections; Escherichia coli; Bacteroides fragilis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300006

Anticonvulsant and proconvulsant roles of nitric oxide in experimental epilepsy models
Provedor de dados: 56
Autores: Del-Bel,E.A.; Oliveira,P.R.; Oliveira,J.A.C.; Mishra,P.K.; Jobe,P.C.; Garcia-Cairasco,N.
The effect of acute (120 mg/kg) and chronic (25 mg/kg, twice a day, for 4 days) intraperitonial injection of the nitric oxide (NO) synthase (NOS) inhibitor NG-nitro-L-arginine (L-NOARG) was evaluated on seizure induction by drugs such as pilocarpine and pentylenetetrazole (PTZ) and by sound stimulation of audiogenic seizure-resistant (R) and audiogenic seizure-susceptible (S) rats. Seizures were elicited by a subconvulsant dose of pilocarpine (100 mg/kg) only after NOS inhibition. NOS inhibition also simultaneously potentiated the severity of PTZ-induced limbic seizures (60 mg/kg) and protected against PTZ-induced tonic seizures (80 mg/kg). The audiogenic seizure susceptibility of S or R rats did not change after similar treatments. In conclusion,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Experimental epilepsy; Seizures; Forebrain; Brainstem; Pilocarpine; Pentylenetetrazol; Audiogenic seizures; Genetically epilepsy-prone rats; GEPR; Nitric oxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800010

Mechanisms of endothelial dysfunction in obesity-associated hypertension
Provedor de dados: 56
Autores: Lobato,N.S.; Filgueira,F.P.; Akamine,E.H.; Tostes,R.C.; Carvalho,M.H.C.; Fortes,Z.B.
Obesity is strongly associated with high blood pressure, dyslipidemia, and type 2 diabetes. These conditions synergistically increase the risk of cardiovascular events. A number of central and peripheral abnormalities can explain the development or maintenance of high blood pressure in obesity. Of great interest is endothelial dysfunction, considered to be a primary risk factor in the development of hypertension. Additional mechanisms also related to endothelial dysfunction have been proposed to mediate the development of hypertension in obese individuals. These include: increase in both peripheral vasoconstriction and renal tubular sodium reabsorption, increased sympathetic activity and overactivation of both the renin-angiotensin system and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypertension; Obesity; Endothelial dysfunction; Oxidative stress; Renin-angiotensin system; Nitric oxide.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500003

Glutathione and the redox control system trypanothione/trypanothione reductase are involved in the protection of Leishmania spp. against nitrosothiol-induced cytotoxicity
Provedor de dados: 56
Autores: Romão,P.R.T.; Tovar,J.; Fonseca,S.G.; Moraes,R.H.; Cruz,A.K.; Hothersall,J.S.; Noronha-Dutra,A.A.; Ferreira,S.H.; Cunha,F.Q.
Glutathione is the major intracellular antioxidant thiol protecting mammalian cells against oxidative stress induced by oxygen- and nitrogen-derived reactive species. In trypanosomes and leishmanias, trypanothione plays a central role in parasite protection against mammalian host defence systems by recycling trypanothione disulphide by the enzyme trypanothione reductase. Although Kinetoplastida parasites lack glutathione reductase, they maintain significant levels of glutathione. The aim of this study was to use Leishmania donovani trypanothione reductase gene mutant clones and different Leishmania species to examine the role of these two individual thiol systems in the protection mechanism against S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Glutathione; Trypanothione; Trypanothione reductase; Nitric oxide; Free radicals.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300006

Arginine induces GH gene expression by activating NOS/NO signaling in rat isolated hemi-pituitaries
Provedor de dados: 56
Autores: Olinto,S.C.F.; Adrião,M.G.; Castro-Barbosa,T.; Goulart-Silva,F.; Nunes,M.T.
The amino acid arginine (Arg) is a recognized secretagogue of growth hormone (GH), and has been shown to induce GH gene expression. Arg is the natural precursor of nitric oxide (NO), which is known to mediate many of the effects of Arg, such as GH secretion. Arg was also shown to increase calcium influx in pituitary cells, which might contribute to its effects on GH secretion. Although the mechanisms involved in the effects of Arg on GH secretion are well established, little is known about them regarding the control of GH gene expression. We investigated whether the NO pathway and/or calcium are involved in the effects of Arg on GH gene expression in rat isolated pituitaries. To this end, pituitaries from approximately 170 male Wistar rats (~250 g) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arginine; GH mRNA expression; Nitric oxide; Calcium (Ca2+); Cyclic guanosine monophosphate.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100011

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction
Provedor de dados: 56
Autores: Bonacorsi,C.; Raddi,M.S.G.; Carlos,I.Z.
Chlorhexidine, even at low concentrations, is toxic for a variety of eukaryotic cells; however, its effects on host immune cells are not well known. We evaluated in vitro chlorhexidine-induced cytotoxicity and its effects on reactive oxygen/nitrogen intermediate induction by murine peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 ml of 10 mM phosphate-buffered saline, washed twice and resuspended (10(6) cells/ml) in appropriate medium for each test. Cell preparations contained more than 95% macrophages. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the presence of hydrogen peroxide (H2O2) and nitric oxide (NO) by the horseradish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chlorhexidine; Cytotoxicity; Macrophages; Nitric oxide; Hydrogen peroxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200007

Role of non-nitric oxide non-prostaglandin endothelium-derived relaxing factor(s) in bradykinin vasodilation
Provedor de dados: 56
Autores: Resende,A.C.; Ballejo,G.; Salgado,M.C.O.
The most conspicuous effect of bradykinin following its administration into the systemic circulation is a transient hypotension due to vasodilation. In the present study most of the available evidence regarding the mechanisms involved in bradykinin-induced arterial vasodilation is reviewed. It has become firmly established that in most species vasodilation in response to bradykinin is mediated by the release of endothelial relaxing factors following the activation of B2-receptors. Although in some cases the action of bradykinin is entirely mediated by the endothelial release of nitric oxide (NO) and/or prostacyclin (PGI2), a large amount of evidence has been accumulated during the last 10 years indicating that a non-NO/PGI2 factor accounts for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prostaglandin; Bradykinin; Nitric oxide; Cytochrome P450; Potassium channels; Endothelium-dependent vasodilation.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900017

Role of the hypothalamic pituitary adrenal axis in the control of the response to stress and infection
Provedor de dados: 56
Autores: McCann,S.M.; Antunes-Rodrigues,J.; Franci,C.R.; Anselmo-Franci,J.A.; Karanth,S.; Rettori,V.
The release of adrenocorticotropin (ACTH) from the corticotrophs is controlled principally by vasopressin and corticotropin-releasing hormone (CRH). Oxytocin may augment the release of ACTH under certain conditions, whereas atrial natriuretic peptide acts as a corticotropin release-inhibiting factor to inhibit ACTH release by direct action on the pituitary. Glucocorticoids act on their receptors within the hypothalamus and anterior pituitary gland to suppress the release of vasopressin and CRH and the release of ACTH in response to these neuropeptides. CRH neurons in the paraventricular nucleus also project to the cerebral cortex and subcortical regions and to the locus ceruleus (LC) in the brain stem. Cortical influences via the limbic system and possibly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corticotropin-releasing hormone (CRH); Vasopressin; Oxytocin; Atrial natriuretic peptide (ANP); ACTH; Cortisol; Norepinephrine; Acetyl choline; Nitric oxide.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000001

Effects of lipopolysaccharide on low- and high-density cultured rabbit vascular smooth muscle cells: differential modulation of nitric oxide release, ERK1/ERK2 MAP kinase activity, protein tyrosine phosphatase activity, and DNA synthesis
Provedor de dados: 56
Autores: Barbosa de Oliveira,L.C.; Rocha Oliveira,C.J.; Fries,D.M.; Stern,A.; Monteiro,H.P.
Previous studies have shown that exogenously generated nitric oxide (NO) inhibits smooth muscle cell proliferation. In the present study, we stimulated rabbit vascular smooth muscle cells (RVSMC) with E. coli lipopolysaccharide (LPS), a known inducer of NO synthase transcription, and established a connection between endogenous NO, phosphorylation/dephosphorylation-mediated signaling pathways, and DNA synthesis. Non-confluent RVSMC were cultured with 0, 5, 10, or 100 ng/ml of the endotoxin. NO release was increased by 86.6% (maximum effect) in low-density cell cultures stimulated with 10 ng/ml LPS as compared to non-stimulated controls. Conversely, LPS (5 to 100 ng/ml) did not lead to enhanced NO production in multilayered (high density) RVSMC. DNA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Lipopolysaccharide; Protein tyrosine phosphatases; Mitogen-activated protein kinases; DNA synthesis; Cell density.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200006

Nitric oxide synthesis and biological functions of nitric oxide released from ruthenium compounds
Provedor de dados: 56
Autores: Pereira,A.C.; Paulo,M.; Araújo,A.V.; Rodrigues,G.J.; Bendhack,L.M.
During three decades, an enormous number of studies have demonstrated the critical role of nitric oxide (NO) as a second messenger engaged in the activation of many systems including vascular smooth muscle relaxation. The underlying cellular mechanisms involved in vasodilatation are essentially due to soluble guanylyl-cyclase (sGC) modulation in the cytoplasm of vascular smooth cells. sGC activation culminates in cyclic GMP (cGMP) production, which in turn leads to protein kinase G (PKG) activation. NO binds to the sGC heme moiety, thereby activating this enzyme. Activation of the NO-sGC-cGMP-PKG pathway entails Ca2+ signaling reduction and vasodilatation. Endothelium dysfunction leads to decreased production or bioavailability of endogenous NO that could...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Vasodilatation; Ruthenium complex; NO-synthase; Resistance artery; Conduit vessel.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900017

Treatment with ascorbic acid and α-tocopherol modulates oxidative-stress markers in the spinal cord of rats with neuropathic pain
Provedor de dados: 56
Autores: Riffel,A.P.K.; Santos,M.C.Q.; de Souza,J.A.; Scheid,T.; Horst,A.; Kolberg,C.; Belló-Klein,A.; Partata,W.A.
Vitamin E (vit. E) and vitamin C (vit. C) are antioxidants that inhibit nociception. The effect of these vitamins on oxidative-stress markers in the spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve is unknown. This study investigated the effect of intraperitoneal administration of vit. E (15 mg·kg-1·day-1) and vit. C (30 mg·kg-1·day-1), given alone or in combination, on spinal cord oxidative-stress markers in CCI rats. Adult male Wistar rats weighing 200–250 g were divided equally into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Total thiol; Superoxide anion generation; Nitric oxide; Lipid hydroperoxides; Hydrogen peroxide; Total antioxidant capacity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400612

Nitro-fatty acids: novel anti-inflammatory lipid mediators
Provedor de dados: 56
Autores: Rubbo,H.
Nitro-fatty acids are formed and detected in human plasma, cell membranes, and tissue, modulating metabolic as well as inflammatory signaling pathways. Here we discuss the mechanisms of nitro-fatty acid formation as well as their key chemical and biochemical properties. The electrophilic properties of nitro-fatty acids to activate anti-inflammatory signaling pathways are discussed in detail. A critical issue is the influence of nitroarachidonic acid on prostaglandin endoperoxide H synthases, redirecting arachidonic acid metabolism and signaling. We also analyze in vivo data supporting nitro-fatty acids as promising pharmacological tools to prevent inflammatory diseases.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitro-fatty acids; Antioxidants; Nitric oxide; Inflammation.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900728

Anxiolytic effect of methylene blue microinjected into the dorsal periaqueductal gray matter
Provedor de dados: 56
Autores: de-Oliveira,R.W.; Guimarães,F.S.
The dorsal periaqueductal gray (DPAG) has been implicated in the behavioral and autonomic expression of defensive reactions. Several results suggest that, along with GABA, glutamate and serotonin, nitric oxide (NO) may play a role in defense reactions mediated by this region. To further investigate this possibility we microinjected methylene blue (MB; 10, 30 or 100 nmol/0.5 µl) into the DPAG of rats submitted to the elevated plus-maze test, an animal model of anxiety. MB has been used as an inhibitor of soluble guanylate cyclase (sGC) to demonstrate cGMP-mediated processes, and there is evidence that NO may exert its biological effects by binding to the heme part of guanylate cyclase, causing an increase in cGMP levels. The results showed that MB (30 nmol)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Anxiety; Elevated plus-maze; Nitric oxide; Animal model.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200012

Increased expression of p38 mitogen- activated protein kinase is related to the acute renal lesions induced by gentamicin
Provedor de dados: 56
Autores: Volpini,R.A.; Balbi,A.P.C.; Costa,R.S.; Coimbra,T.M.
Mitogen-activated protein kinases (MAPK) may be involved in the pathogenesis of acute renal failure. This study investigated the expression of p-p38 MAPK and nuclear factor kappa B (NF-kappaB) in the renal cortex of rats treated with gentamicin. Twenty rats were injected with gentamicin, 40 mg/kg, im, twice a day for 9 days, 20 with gentamicin + pyrrolidine dithiocarbamate (PDTC, an NF-kappaB inhibitor), 14 with 0.15 M NaCl, im, twice a day for 9 days, and 14 with 0.15 M NaCl , im, twice a day for 9 days and PDTC, 50 mg kg-1 day-1, ip, twice a day for 15 days. The animals were killed 5 and 30 days after the last of the injections and the kidneys were removed for histological, immunohistochemical and Western blot analysis and for nitrate determination. The...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: P38 mitogen-activated protein kinase; Nuclear factor-kappaB; Inflammation; Renal fibrosis; Gentamicin-induced acute tubular necrosis; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000600016

Role of nitric oxide and superoxide in Giardia lamblia killing
Provedor de dados: 56
Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (&amp;gt;90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015

Antagonism by hemoglobin of effects induced by L-arginine in neuromuscular preparations from rats
Provedor de dados: 56
Autores: Ambiel,C.R.; Alves-Do-Prado,Wilson.
Nitric oxide (NO)-synthase is present in diaphragm, phrenic nerve and vascular smooth muscle. It has been shown that the NO precursor L-arginine (L-Arg) at the presynaptic level increases the amplitude of muscular contraction (AMC) and induces tetanic fade when the muscle is indirectly stimulated at low and high frequencies, respectively. However, the precursor in muscle reduces AMC and maximal tetanic fade when the preparations are stimulated directly. In the present study the importance of NO synthesized in different tissues for the L-Arg-induced neuromuscular effects was investigated. Hemoglobin (50 nM) did not produce any neuromuscular effect, but antagonized the increase in AMC and tetanic fade induced by L-Arg (9.4 mM) in rat phrenic nerve-diaphragm...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skeletal muscle; Nitric oxide; Tetanic fade; L-arginine; D-arginine; Hemoglobin.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400017

Local inhibition of nitrergic activity in tenotomized rats accelerates muscle regeneration by increasing fiber area and decreasing central core lesions
Provedor de dados: 56
Autores: Seabra,A.D.; Moraes,S.A.S.; Batista,E.J.O.; Garcia,T.B.; Souza,M.C.; Oliveira,K.R.M.; Herculano,A.M.
Muscular atrophy is a progressive degeneration characterized by muscular proteolysis, loss of mass and decrease in fiber area. Tendon rupture induces muscular atrophy due to an intrinsic functional connection. Local inhibition of nitric oxide synthase (NOS) by Nω-nitro-L-arginine methyl ester (L-NAME) accelerates tendon histological recovery and induces functional improvement. Here we evaluate the effects of such local nitrergic inhibition on the pattern of soleus muscle regeneration after tenotomy. Adult male Wistar rats (240 to 280 g) were divided into four experimental groups: control (n=4), tenotomized (n=6), vehicle (n=6), and L-NAME (n=6). Muscular atrophy was induced by calcaneal tendon rupture in rats. Changes in muscle wet weight and total protein...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Muscle regeneration; Atrophy; Tendon; Tenotomy.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300601

Participation of nitric oxide in the nucleus isthmi in CO2-drive to breathing in toads
Provedor de dados: 56
Autores: Gargaglioni,L.H.; Branco,L.G.S.
The nucleus isthmi (NI) is a mesencephalic structure of the amphibian brain. It has been reported that NI plays an important role in integration of CO2 chemoreceptor information and glutamate is probably involved in this function. However, very little is known about the mechanisms involved. Recently, it has been shown that nitric oxide synthase (NOS) is expressed in the brain of the frog. Thus the gas nitric oxide (NO) may be involved in different functions in the brain of amphibians and may act as a neurotransmitter or neuromodulator. We tested the hypothesis that NO plays a role in CO2-drive to breathing, specifically in the NI comparing pulmonary ventilation, breathing frequency and tidal volume, after microinjecting 100 nmol/0.5 µl of L-NAME (a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Amphibian; Hypercapnia; Ventilation; Nucleus isthmi; Toad; CO2; L-NAME.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100010

Purine nucleotides reduce superoxide production by nitric oxide synthase in a murine sepsis model
Provedor de dados: 56
Autores: Barbeiro,H.V.; Barbeiro,D.F.; Debbas,V.; Souza,H.P.; Laurindo,F.R.; Velasco,I.T.; Soriano,F.G.
Sepsis involves a systemic inflammatory response of multiple endogenous mediators, resulting in many of the injurious and sometimes fatal physiological symptoms of the disease. This systemic activation leads to a compromised vascular response and endothelial dysfunction. Purine nucleotides interact with purinoceptors and initiate a variety of physiological processes that play an important role in maintaining cardiovascular function. The purpose of the present study was to investigate the effects of ATP on vascular function in a lipopolysaccharide (LPS) model of sepsis. LPS induced a significant increase in aortic superoxide production 16 h after injection. Addition of ATP to the organ bath incubation solution reduced superoxide production by the aortas of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: ATP; Endotoxin; Endothelium; Superoxide; Nitric oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100009

Contrasting effects of nitric oxide and corticotropin- releasing factor within the dorsal periaqueductal gray on defensive behavior and nociception in mice
Provedor de dados: 56
Autores: Miguel,T.T.; Gomes,K.S.; Nunes-de-Souza,R.L.
The anxiogenic and antinociceptive effects produced by glutamate N-methyl-D-aspartate receptor activation within the dorsal periaqueductal gray (dPAG) matter have been related to nitric oxide (NO) production, since injection of NO synthase (NOS) inhibitors reverses these effects. dPAG corticotropin-releasing factor receptor (CRFr) activation also induces anxiety-like behavior and antinociception, which, in turn, are selectively blocked by local infusion of the CRF type 1 receptor (CRFr1) antagonist, NBI 27914 [5-chloro-4-(N-(cyclopropyl)methyl-N-propylamino)-2-methyl-6-(2,4,6-trichlorophenyl)aminopyridine]. Here, we determined whether i) the blockade of the dPAG by CRFr1 attenuates the anxiogenic/antinociceptive effects induced by local infusion of the NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Corticotropin-releasing factor; Periaqueductal gray; Defensive behavior; Antinociception; Mice.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000400003

Evidence of interaction between fluoxetine and isosorbide dinitrate on neuroleptic-induced catalepsy in mice
Provedor de dados: 56
Autores: Pires,J.G.P.; Fonseca,F.C.G.; Woelffel,A.B.; Futuro-Neto,H.A.
Drugs which influence 5-HTergic mechanisms can modify neuroleptic-induced catalepsy (NC) in rodents, a phenomenon produced by striatal dopamine (DA) receptor blockade. Previous research also suggests a role for endogenous nitric oxide (NO) in the modulation of striatal DAergic neurotransmission; in addition, NO seems to play a role in the 5-HT reuptake mechanism. It is known that clomipramine potentiates NC in mice, but the reported effects of selective 5-HT reuptake inhibitors (SSRIs) in this model are rather contradictory. We then decided to re-address this issue, investigating the effect of fluoxetine (FX), an SSRI, on NC. In view of the ubiquitous role of NO as a central neuromodulator, we also studied the effect of isosorbide dinitrate (ID), a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fluoxetine; Isosorbide dinitrate; Neuroleptic-induced catalepsy; Nitric oxide; Serotonin reuptake inhibitors; Dopaminergic transmission.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300014

Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications
Provedor de dados: 56
Autores: Guidoni,M.; de Christo Scherer,M.M.; Figueira,M.M.; Schmitt,E.F.P.; de Almeida,L.C.; Scherer,R.; Bogusz,S.; Fronza,M.
Vegetable oils have been used for a plethora of health benefits by their incorporation in foods, cosmetics, and pharmaceutical products, especially those intended for skin care. This study aimed to investigate the cutaneous benefits of a vegetable oil blend (VOB) formulation and its fatty acid composition. The anti-inflammatory activity was studied in macrophages of RAW 264.7 cells by investigating the release of nitric oxide (NO), superoxide anion generation (O2-), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6). ABTS cation radical scavenging capacity assay, ferric reducing antioxidant potential (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and NO free radical scavenging assays were used to evaluate the antioxidant activity. VOB was tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vegetable oil blend; Skin care; Fatty acids; Anti-inflammatory; Nitric oxide; Scratch assay.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200609

Novel donors of nitric oxide derived of S-nitrosocysteine possessing antioxidant activities
Provedor de dados: 56
Autores: Petit,C.; Bernardes-Genisson,V.; Hoffmann,P.; Souchard,J.-P.; Labidalle,S.
Novel S-nitrosothiols possessing a phenolic function were investigated as nitric oxide (NO) donors. A study of NO release from these derivatives was carried out by electron spin resonance (ESR). All compounds gave rise to a characteristic three-line ESR signal in the presence of the complex [Fe(II)(MGD)2], revealing the formation of the complex [Fe(II)(MGD)2(NO)]. Furthermore, tests based on cytochrome c reduction were performed in order to study the ability of each phenolic disulfide, the final organic decomposition product of S-nitrosothiols, to trap superoxide radical anion (O2-). This study revealed a high reactivity of 1b and 3b towards O2-. For these two compounds, the respective inhibitory concentration (IC) 50 values were 92 µM and 43 µM.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; S-nitrosothiols (thionitrites); Antioxidant; Peroxynitrite.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100011

4-Aminopyridine inhibits the neuromuscular effects of nitric oxide and 8-Br-cGMP
Provedor de dados: 56
Autores: Queiroz,R.N.; Ramos,E.R.P.; Alves-Do-Prado,W.
The effects induced by nitric oxide (NO) in different tissues depend on direct and/or indirect interactions with K+ channels. The indirect interaction of NO is produced by activation of guanylyl cyclase which increases the intracellular cGMP. Since NO, cGMP and 4-aminopyridine alone induce tetanic fade and increase amplitude of muscular contractions in isolated rat neuromuscular preparations, the present study was undertaken to determine whether or not the neuromuscular effects of NO and 8-Br-cGMP can be modified by 4-aminopyridine. Using the phrenic nerve and diaphragm muscle isolated from male Wistar rats (200-250 g), we observed that L-arginine (4.7 mM) and 8-Br-cGMP (18 µM), in contrast to D-arginine, induced an increase in the amplitude of muscle...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: L-arginine; Nitric oxide; CGMP; Skeletal muscle; Tetanic fade; K+ channel.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700016

Protein 3-nitrotyrosine formation during Trypanosoma cruzi infection in mice
Provedor de dados: 56
Autores: Naviliat,M.; Gualco,G.; Cayota,A.; Radi,R.
Nitric oxide (·NO) is a diffusible messenger implicated in Trypanosoma cruzi resistance. Excess production of ·NO and oxidants leads to the generation of nitrogen dioxide (·NO2), a strong nitrating agent. Tyrosine nitration is a post-translational modification resulting from the addition of a nitro (-NO2) group to the ortho-position of tyrosine residues. Detection of protein 3-nitrotyrosine is regarded as a marker of nitro-oxidative stress and is observed in inflammatory processes. The formation and role of nitrating species in the control and myocardiopathy of T. cruzi infection remain to be studied. We investigated the levels of ·NO and protein 3-nitrotyrosine in the plasma of C3H and BALB/c mice and pharmacologically modulated their production during...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Peroxynitrite; Nitric oxide; Nitrotyrosine; Free radicals; Trypanosoma cruzi; Chagas' disease.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200011

Uptake of NO-releasing drugs by the P2 nucleoside transporter in trypanosomes
Provedor de dados: 56
Autores: Soulère,L.; Hoffmann,P.; Bringaud,F.; Périé,J.
Nitric oxide (NO·) has been identified as a principal regulatory molecule of the immune system and the major cytotoxic mediator of activated immune cells. NO· can also react rapidly with a variety of biological species, particularly with the superoxide radical anion O2·- at almost diffusion-limited rates to form peroxynitrite anion (ONOO-). ONOO- and its proton-catalyzed decomposition products are capable of oxidizing a great diversity of biomolecules and can act as a source of toxic hydroxyl radicals. As a consequence, a strategy for the development of molecules with potential trypanocidal activities could be developed to increase the concentration of nitric oxide in the parasites through NO·-releasing compounds. In this way, the rate of formation of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Peroxynitrite; Thionitrite; S-nitrosothiol; Adenosine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100016

Urinary NO3 excretion and renal failure in indinavir-treated patients
Provedor de dados: 56
Autores: Eira,M.; Araujo,M.; Seguro,A.C.
Treatment with indinavir (IDV), a protease inhibitor, is frequently associated with renal abnormalities. We determined the incidence of renal failure (creatinine clearance &lt;80 mL min-1 1.73 (m²)-1) in HIV patients treated with highly active antiretroviral therapy, including IDV, and investigated the possible mechanisms and risk factors of IDV nephrotoxicity. Thirty-six patients receiving IDV were followed for 3 years. All were assessed for age, body weight, duration of infection, duration of IDV treatment, sulfur-derivative use, total cholesterol, triglycerides, magnesium, sodium, potassium, creatinine, and urinalysis. We also determined renal function in terms of creatinine clearance, urine osmolality and fractional excretion of sodium, potassium, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Indinavir; Nephrotoxicity; Nitric oxide.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800009

A method for multiple sequential analyses of macrophage functions using a small single cell sample
Provedor de dados: 56
Autores: Nascimento,F.R.F.; Rodríguez,D.; Gomes,E.; Fernvik,E.C.; Russo,M.
Microbial pathogens such as bacillus Calmette-Guérin (BCG) induce the activation of macrophages. Activated macrophages can be characterized by the increased production of reactive oxygen and nitrogen metabolites, generated via NADPH oxidase and inducible nitric oxide synthase, respectively, and by the increased expression of major histocompatibility complex class II molecules (MHC II). Multiple microassays have been developed to measure these parameters. Usually each assay requires 2-5 x 10(5) cells per well. In some experimental conditions the number of cells is the limiting factor for the phenotypic characterization of macrophages. Here we describe a method whereby this limitation can be circumvented. Using a single 96-well microassay and a very small...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Macrophages; Nitric oxide; Hydrogen peroxide; MHC II; Method.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900012

Reversal by methylene blue of tetanic fade induced in cats by nitric oxide
Provedor de dados: 56
Autores: Ambiel,C.R.; Alves-Do-Prado,W.
Previous data from our laboratory have indicated that nitric oxide (NO) acting at the presynaptic level increases the amplitude of muscular contraction (AMC) of the phrenic-diaphragm preparations isolated from indirectly stimulated rats, but, by acting at the postsynaptic level, it reduces the AMC when the preparations are directly stimulated. In the present study we investigated the effects induced by NO when tetanic frequencies of stimulation were applied to in vivo preparations (sciatic nerve-anterior tibial muscle of the cat). Intra-arterial injection of NO (0.75-1.5 mg/kg) induced a dose-dependent increase in the Wedensky inhibition produced by high frequencies of stimulation applied to the motor nerve. Intra-arterial administration of 7.2 µg/kg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wedensky inhibition; Nitric oxide; Methylene blue; Skeletal muscle.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300013

Early and late neuropathological features of meningoencephalitis associated with Maraba virus infection
Provedor de dados: 56
Autores: Maia-Farias,A.; Lima,C.M.; Freitas,P.S.L.; Diniz,D.G.; Rodrigues,A.P.D.; Quaresma,J.A.S.; Diniz,C.W. Picanço; Diniz,J.A.
Maraba virus is a member of the genus Vesiculovirus of the Rhabdoviridae family that was isolated in 1983 from sandflies captured in the municipality of Maraba, state of Pará, Amazônia, Brazil. Despite 30 years having passed since its isolation, little is known about the neuropathology induced by the Maraba virus. Accordingly, in this study the histopathological features, inflammatory glial changes, cytokine concentrations, and nitric oxide activity in the encephalon of adult mice subjected to Maraba virus nostril infection were evaluated. The results showed that 6 days after intranasal inoculation, severe neuropathological-associated disease signs appeared, including edema, necrosis and pyknosis of neurons, generalized congestion of encephalic vessels,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maraba virus; Vesiculovirus; Microglia; Astrocytes; Cytokines; Nitric oxide.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400610

Nitric oxide modulates ATP-evoked currents in mouse Leydig cells
Provedor de dados: 56
Autores: de Deus,J.L.; Dagostin,A.L.A.; Varanda,W.A.
Testosterone synthesis within Leydig cells is a calcium-dependent process. Intracellular calcium levels are regulated by different processes including ATP-activated P2X purinergic receptors, T-type Ca2+ channels modulated by the luteinizing hormone, and intracellular calcium storages recruited by a calcium-induced calcium release mechanism. On the other hand, nitric oxide (NO) is reported to have an inhibitory role in testosterone production. Based on these observations, we investigated the interaction between the purinergic and nitrergic systems in Leydig cells of adult mice. For this purpose, we recorded ATP-evoked currents in isolated Leydig cells using the whole cell patch clamp technique after treatment with L-NAME (300 μM and 1 mM), L-arginine (10,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leydig cells; ATP; Purinergic receptor; Calcium; Nitric oxide; Whole cell patch clamp.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500603

Nitric oxide synthase blockade and body fluid volumes
Provedor de dados: 56
Autores: Balaszczuk,A.M.; Tomat,A.; Bellucci,S.; Fellet,A.; Arranz,C.
The influence of chronic nitric oxide synthase inhibition with N G-nitro-L-arginine methyl ester (L-NAME) on body fluid distribution was studied in male Wistar rats weighing 260-340 g. Extracellular, interstitial and intracellular spaces, as well as plasma volume were measured after a three-week treatment with L-NAME (~70 mg/kg per 24 h in drinking water). An increase in extracellular space (16.1 ± 1.1 vs 13.7 ± 0.6 ml/100 g in control group, N = 12, P&lt;0.01), interstitial space (14.0 ± 0.9 vs 9.7 ± 0.6 ml/100 g in control group, P&lt;0.001) and total water (68.7 ± 3.9 vs 59.0 ± 2.9 ml/100 g, P&lt;0.001) was observed in the L-NAME group (N = 8). Plasma volume was lower in L-NAME-treated rats (2.8 ± 0.2 ml/100 g) than in the control group (3.6 ± 0.1...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Kidney; Heart; Body fluid; Hypertension; Extracellular space; Intracellular space; L-NAME.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100019

Endothelium-dependent vasodilation in response to Pseudomonas aeruginosa lipopolysaccharide: an in vitro study on canine arteries
Provedor de dados: 56
Autores: Evora,P.R.B.; Ekin,S.; Pearson,P.J.; Schaff,H.V.
Early systemic arterial hypotension is a common clinical feature of Pseudomonas septicemia. To determine if Pseudomonas aeruginosa endotoxin induces the release of endothelium-derived nitric oxide (EDNO), an endogenous nitrovasodilator, segments of canine femoral, renal, hepatic, superior mesenteric, and left circumflex coronary arteries were suspended in organ chambers (physiological salt solution, 95% O2/5% CO2, pH 7.4, 37oC) to measure isometric force. In arterial segments contracted with 2 µM prostaglandin F2&lt;FONT FACE="Symbol"&gt;a&lt;/font&gt;, Pseudomonas endotoxin (lipopolysaccharide (LPS) serotype 10(Habs) from Pseudomonas aeruginosa (0.05 to 0.50 mg/ml)) induced concentration-dependent relaxation of segments with endothelium (P&amp;lt;0.05)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelium-derived relaxing factor; EDRF; Nitric oxide; Hypotension; Sepsis.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000014

Do endogenous opioids and nitric oxide participate in the anticonvulsant action of dipyrone?
Provedor de dados: 56
Autores: Reis,G.M.L.; Doretto,M.C.; Duarte,I.D.G.; Tatsuo,M.A.K.F.
It was previously reported that systemic administration of dipyrone inhibited the tonic component of generalized tonic-clonic seizures in both the electroshock and the audiogenic seizure models. The aim of the present study was to investigate the mechanisms involved in the anticonvulsant action of dipyrone by assessing the role of nitric oxide and opioids in the electroshock (female 60- to 90-day-old Wistar rats, N = 5-11) and audiogenic seizure (female 60- to 90-day-old Wistar audiogenic rats, N = 5-11) models of epilepsy. Naloxone (5 mg/kg, sc) significantly reversed the anticonvulsant effect of dipyrone in rats submitted to the induction of audiogenic seizures (ANOVA/Bonferroni's test), suggesting the involvement of opioid peptides in this action. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epilepsy; Dipyrone; Audiogenic seizures; Electroshock; Nitric oxide; Opiates.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900018

Reduction of blood nitric oxide levels is associated with clinical improvement of the chronic pelvic pain related to endometriosis
Provedor de dados: 56
Autores: Rocha,M.G.; Gomes,V.A.; Tanus-Santos,J.E.; Rosa-e-Silva,J.C.; Candido-dos-Reis,F.J.; Nogueira,A.A.; Poli-Neto,O.B.
The objective of this prospective study was to determine the plasma levels of nitric oxide (NO) in women with chronic pelvic pain secondary to endometriosis (n=24) and abdominal myofascial pain syndrome (n=16). NO levels were measured in plasma collected before and 1 month after treatment. Pretreatment NO levels (μM) were lower in healthy volunteers (47.0±12.7) than in women with myofascial pain (64.2±5.0, P=0.01) or endometriosis (99.5±12.9, P&lt;0.0001). After treatment, plasma NO levels were reduced only in the endometriosis group (99.5±12.9 vs 61.6±5.9, P=0.002). A correlation between reduction of pain intensity and reduction of NO level was observed in the endometriosis group [correlation = 0.67 (95%CI = 0.35 to 0.85), P&lt;0.0001]. Reduction of NO...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chronic pelvic pain; Endometriosis; Abdominal myofascial pain syndrome; Nitric oxide; Inflammation; Central sensitization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400363

Antinociceptive effect on mice of the hydroalcoholic fraction and (-) epicatechin obtained from Combretum leprosum Mart &amp;amp; Eich
Provedor de dados: 56
Autores: Lopes,L.S.; Marques,R.B.; Pereira,S.S.; Ayres,M.C.C.; Chaves,M.H.; Cavalheiro,A.J.; Vieira Júnior,G.M.; Almeida,F.R.C.
Previous studies on Combretum leprosum, a tree growing in the Northeastern states of Brazil, have shown antinociceptive effects of the ethanol extract of its leaves and bark, but studies examining its constituents are rare. The objective of this study was to evaluate the antinociceptive effect of the hydroalcoholic fraction (HF) of one of its constituents, the flavonoid (-) epicatechin (EPI), administered orally to mice (20-30 g) in models of chemical nociception, and the possible mechanisms involved. Different doses of HF (62.5 to 500 mg/kg) and EPI (12.5 to 50 mg/kg) were evaluated in models of abdominal writhing, glutamate, capsaicin, and formalin in animals pretreated with different antagonists: naloxone, ondansetron, yohimbine, ketanserin, pindolol,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Combretum leprosum; Epicatechin; Antinociceptive; Serotonin; Nitric oxide.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200008

Oxidative stress may explain how hypertension is maintained by normal levels of angiotensin II
Provedor de dados: 56
Autores: Romero,J.C.; Reckelhoff,J.F.
It is well known that essential hypertension evolves in most patients with "near normal" levels of plasma renin activity. However, these levels appear to be responsible for the high levels of arterial pressure because they are normalized by the administration of angiotensin II converting inhibitors or angiotensin receptor antagonist. In experimental animals, hypertension can be induced by the continuous intravenous infusion of small doses of angiotensin II that are not sufficient to evoke an immediate pressor response. However, this condition resembles the characteristics of essential hypertension because the high levels of blood pressure exist with normal plasma levels of angiotensin II. It is suggested that small amounts of angiotensin whose plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Isoprostanes; Peroxynitrites; Essential hypertension.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000600006

Butyrate induces apoptosis in murine macrophages via caspase-3, but independent of autocrine synthesis of tumor necrosis factor and nitric oxide
Provedor de dados: 56
Autores: Ramos,M.G.; Rabelo,F.L.A.; Duarte,T.; Gazzinelli,R.T.; Alvarez-Leite,J.I.
We demonstrated that 4 mM butyrate induces apoptosis in murine peritoneal macrophages in a dose- and time-dependent manner as indicated by studies of cell viability, flow cytometric analysis of annexin-V binding, DNA ladder pattern and the determination of hypodiploid DNA content. The activity of caspase-3 was enhanced during macrophage apoptosis induced by butyrate and the caspase inhibitor z-VAD-FMK (100 µM) inhibited the butyrate effect, indicating the major role of the caspase cascade in the process. The levels of butyrate-induced apoptosis in macrophages were enhanced by co-treatment with 1 µg/ml bacterial lipopolysaccharide (LPS). However, our data indicate that apoptosis induced by butyrate and LPS involves different mechanisms. Thus, LPS-induced...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Butyrate; Apoptosis; Caspase; Nitric oxide; Cytokine; Macrophage.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200004

Impact of chronic administration of anabolic androgenic steroids and taurine on blood pressure in rats
Provedor de dados: 56
Autores: Roşca,A.E.; Stoian,I.; Badiu,C.; Gaman,L.; Popescu,B.O.; Iosif,L.; Mirica,R.; Tivig,I.C.; Stancu,C.S.; Căruntu,C.; Voiculescu,S.E.; Zăgrean,L.
Supraphysiological administration of anabolic androgenic steroids has been linked to increased blood pressure. The widely distributed amino acid taurine seems to be an effective depressor agent in drug-induced hypertension. The purpose of this study was to assess the impact of chronic high dose administration of nandrolone decanoate (DECA) and taurine on blood pressure in rats and to verify the potentially involved mechanisms. The study was conducted in 4 groups of 8 adult male Wistar rats, aged 14 weeks, treated for 12 weeks with: DECA (A group); vehicle (C group); taurine (T group), or with both drugs (AT group). Systolic blood pressure (SBP) was measured at the beginning of the study (SBP1), 2 (SBP2) and 3 months (SBP3) later. Plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Anabolic androgenic steroids; Nandrolone decanoate; Taurine; Blood pressure; Angiotensin-converting enzyme; Nitric oxide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000600706

Constitutive nitric oxide synthase (cNOS) activity in Langerhans islets from streptozotocin diabetic rats
Provedor de dados: 56
Autores: Fonovich de Schroeder,T.M.; Carattino,M.D.; Frontera,M.; Catanzaro,O.L.
Nitric oxide synthase activity was measured in Langerhans islets isolated from control and streptozotocin diabetic rats. The activity of the enzyme was linear up to 150 µg of protein from control rats and was optimal at 0.1 µM calcium, when it was measured after 45 min of incubation at 37oC in the presence of 200 µM arginine. Specific activity of the enzyme was 25 x 10-4 nmol [3H]citrulline 45 min-1 mg protein-1. Streptozotocin diabetic rats exhibited less enzyme activity both in total pancreas homogenate and in isolated Langerhans islets when compared to control animals. Nitric oxide synthase activity measured in control and diabetic rats 15 days after the last streptozotocin injection in the second group of animals corresponded only to a constitutive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide synthase; Nitric oxide; Langerhans islets; Streptozotocin; Diabetes.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000500004

Effect of psychological stress on the L-arginine-nitric oxide pathway and semen quality
Provedor de dados: 56
Autores: Eskiocak,S.; Gozen,A.S.; Taskiran,A.; Kilic,A.S.; Eskiocak,M.; Gulen,S.
It has been reported that mental stress causes abnormality of spermiogram parameters. We investigated the effect of psychological stress on the L-arginine-nitric oxide (NO) pathway. Semen samples were collected from 29 healthy fourth semester medical students just before (stress) and 3 months after (non-stress) the final examinations. Psychological stress was measured by the State Anxiety Inventory questionnaire. After standard semen analysis, arginase activity and NO concentration were measured spectrophotometrically in the seminal plasma. Measurements were made in duplicate. During the stress period, sperm concentration (41.28 ± 3.70 vs 77.62 ± 7.13 x 10(6)/mL), rapid progressive motility of spermatozoa (8.79 ± 1.66 vs 20.86 ± 1.63%) and seminal plasma...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Psychological stress; Arginase; Nitric oxide; Sperm motility; Sperm count.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500003

Atropine and ODQ antagonize tetanic fade induced by L-arginine in cats
Provedor de dados: 56
Autores: Cruciol-Souza,J.M.; Alves-Do-Prado,W.
Although it has been demonstrated that nitric oxide (NO) released from sodium nitrite induces tetanic fade in the cat neuromuscular preparations, the effect of L-arginine on tetanic fade and its origin induced by NO have not been studied in these preparations. Furthermore, atropine reduces tetanic fade induced by several cholinergic and anticholinergic drugs in these preparations, whose mechanism is suggested to be mediated by the interaction of acetylcholine with inhibitory presynaptic muscarinic receptors. The present study was conducted in cats to determine the effects of L-arginine alone or after pretreatment with atropine or 1H-[1,2,4]oxadiazole [4,3-a]quinoxalin-1-one (ODQ) on neuromuscular preparations indirectly stimulated at high frequency. Drugs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atropine; Tetanic fade; Skeletal muscle; Nitric oxide; Muscarinic receptors.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001000015

Roles of calcium and IP3 in impaired colon contractility of rats following multiple organ dysfunction syndrome
Provedor de dados: 56
Autores: Zheyu,C.; Qinghui,Q.; Lunan,Y.
The purpose of the present study was to explore changes in rat colon motility, and determine the roles of calcium and inositol (1,4,5)-triphosphate (IP3) in colon dysmotility induced by multiple organ dysfunction syndrome (MODS) caused by bacteria peritonitis. The number of stools, the contractility of the muscle strips and the length of smooth muscle cells (SMC) in the colon, the concentration of calcium and IP3 in SMC, and serum nitric oxide were measured. Number of stools, fecal weight, IP3 concentration in SMC and serum nitric oxide concentration were 0.77 ± 0.52 pellets, 2.51 ± 0.39 g, 4.14 ± 2.07 pmol/tube, and 113.95 ± 37.89 µmol/L, respectively, for the MODS group (N = 11) vs 1.54 ± 0.64 pellets, 4.32 ± 0.57 g, 8.19 ± 3.11 pmol/tube, and 37.42 ±...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Calcium; Smooth muscle; IP3; Nitric oxide.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000012

Ionic radiocontrast inhibits endothelium-dependent vasodilation of the canine renal artery in vitro: possible mechanism of renal failure following contrast medium infusion
Provedor de dados: 56
Autores: Discigil,B.; Evora,P.R.B.; Pearson,P.J.; Viaro,F.; Rodrigues,A.J.; Schaff,H.V.
To determine if radiocontrast impairs vascular relaxation of the renal artery, segments (4-5 mm in length) of canine renal artery were suspended in vitro in organ chambers to measure isometric force (95% O2/5% CO2, at 37ºC). Arterial segments with and without endothelium were placed at the optimal point of their length-tension relation and incubated with 10 µM indomethacin to prevent synthesis of endogenous prostanoids. The presence of nonionic radiocontrast (iohexol, Omnipaque 350, 1 ml in 25 ml control solution, 4% (v/v)) did not alter endothelium-dependent relaxation to acetylcholine in rings precontracted with both norepinephrine and prostaglandin F2alpha (N = 6). When the rings were precontracted with prostaglandin F2alpha, the presence of ionic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Radiocontrast; Renal failure; Nitric oxide; Vasodilation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000200014

Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
Provedor de dados: 56
Autores: Goldstein,J.; Silberstein,C.; Ibarra,C.
Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic AMP from ATP. These isoforms are critically involved in the regulation of gene transcription, metabolism, and ion channel activity among others. Nitric oxide (NO) is a gaseous product whose synthesis from L-arginine is catalyzed by the enzyme NO synthase. It has been well established that NO activates the enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such as AC. In the present study, the effects of sodium nitroprusside (SNP), a nitric oxide-releasing compound, on COS-7 cells transfected with plasmids containing AC types I, II, V and VI were evaluated. Total inhibition (~98.5%) of cAMP production was observed in COS-7...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adenylyl cyclase; Nitric oxide; Sodium nitroprusside; Cyclic AMP; COS-7 cells; Signal transduction.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200002

High plasma concentrations of asymmetric dimethylarginine inhibit ischemic cardioprotection in hypercholesterolemic rats
Provedor de dados: 56
Autores: Landim,M.B.P.; Dourado,P.M.M.; Casella-Filho,A.; Chagas,A.C.P.; da-Luz,P.L.
A low concentration of nitric oxide associated with a high concentration of asymmetric dimethylarginine (ADMA) can explain the lack of ischemic cardioprotection observed in the presence of hypercholesterolemia. The objective of the present study was to evaluate the effect of hypercholesterolemia on ischemic pre- and postconditioning and its correlation with plasma concentrations of ADMA. Male Wistar rats (6-8 weeks old) fed a 2% cholesterol diet (n = 21) for 8 weeks were compared to controls (n = 25) and were subjected to experimental myocardial infarction and reperfusion, with ischemic pre- and postconditioning. Total cholesterol and ADMA were measured in plasma before the experimental infarct and the infarct area was quantified. Weight, total cholesterol...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hypercholesterolemia; Cardiotonics; Nitric oxide; Risk factors; Asymmetric dimethylarginine.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000500454

Effect of inhibition of nitric oxide synthase on blood pressure and renal sodium handling in renal denervated rats
Provedor de dados: 56
Autores: Xavier,F.; Magalhães,A.M.F.; Gontijo,J.A.R.
The role of sympathetic nerve activity in the changes in arterial blood pressure and renal function caused by the chronic administration of NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, was examined in sham and bilaterally renal denervated rats. Several studies have demonstrated that sympathetic nerve activity is elevated acutely after L-NAME administration. To evaluate the role of renal nerve activity in L-NAME-induced hypertension, we compared the blood pressure response in four groups (N = 10 each) of male Wistar-Hannover rats weighing 200 to 250 g: 1) sham-operated vehicle-treated, 2) sham-operated L-NAME-treated, 3) denervated vehicle-treated, and 4) denervated L-NAME-treated rats. After renal denervation or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Hypertension; Natriuresis; Renal denervation; Lithium clearance.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300014

Immunological and biochemical parameters of patients with metabolic syndrome and the participation of oxidative and nitroactive stress
Provedor de dados: 56
Autores: Simão,A.N.C.; Lozovoy,M.A.B.; Simão,T.N.C.; Venturini,D.; Barbosa,D.S.; Dichi,J.B.; Matsuo,T.; Cecchini,R.; Dichi,I.
Metabolic syndrome (MS) is a multifactorial disease involving inflammatory activity and endothelial dysfunction. The aim of the present study was to evaluate the relationship between the changes in lipoperoxidation, in immunological and biochemical parameters and nitric oxide metabolite (NOx) levels in MS patients. Fifty patients with MS (4 males/46 females) and 50 controls (3 males/47 females) were studied. Compared to control (Mann-Whitney test), MS patients presented higher serum levels (P &lt; 0.05) of fibrinogen: 314 (185-489) vs 262 (188-314) mg/dL, C-reactive protein (CRP): 7.80 (1.10-46.50) vs 0.70 (0.16-5.20) mg/dL, interleukin-6: 3.96 (3.04-28.18) vs 3.33 (2.55-9.63) pg/mL, uric acid: 5.45 (3.15-9.65) vs 3.81 (2.70-5.90) mg/dL, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metabolic syndrome; Uric acid; Inflammation; Adiponectin; Nitric oxide; Lipoperoxidation.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000700015

Stress-induced neuroinflammation: mechanisms and new pharmacological targets
Provedor de dados: 56
Autores: Munhoz,C.D.; García-Bueno,B.; Madrigal,J.L.M.; Lepsch,L.B.; Scavone,C.; Leza,J.C.
Stress is triggered by numerous unexpected environmental, social or pathological stimuli occurring during the life of animals, including humans, which determine changes in all of their systems. Although acute stress is essential for survival, chronic, long-lasting stress can be detrimental. In this review, we present data supporting the hypothesis that stress-related events are characterized by modifications of oxidative/nitrosative pathways in the brain in response to the activation of inflammatory mediators. Recent findings indicate a key role for nitric oxide (NO) and an excess of pro-oxidants in various brain areas as responsible for both neuronal functional impairment and structural damage. Similarly, cyclooxygenase-2 (COX-2), another known source of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cyclooxygenase; Nitric oxide; PPARγ; Stress-induced neurodegeneration.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200001

Chronic hypertension alters the expression of Cx43 in cardiovascular muscle cells
Provedor de dados: 56
Autores: Haefliger,J.-A.; Meda,P.
Connexin43 (Cx43), the predominant gap junction protein of muscle cells in vessels and heart, is involved in the control of cell-to-cell communication and is thought to modulate the contractility of the vascular wall and the electrical coupling of cardiac myocytes. We have investigated the effects of arterial hypertension on the expression of Cx43 in aorta and heart in three different models of experimental hypertension. Rats were made hypertensive either by clipping one renal artery (two kidney, one-clip renal (2K,1C) model) by administration of deoxycorticosterone and salt (DOCA-salt model) or by inhibiting nitric oxide synthase with NG-nitro-L-arginine methyl ester (L-NAME model). After 4 weeks, rats of the three models showed a similar increase in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-cell communication; Gap junctions; Connexins; Vasculature; Heart; Hypertension; Blood pressure; Renin; Deoxycorticosterone; Nitric oxide; Distensibility.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400009

Effect of acute nitric oxide synthase inhibition in the modulation of heart rate in rats
Provedor de dados: 56
Autores: Fellet,A.L.; Di Verniero,C.; Arza,P.; Tomat,A.; Varela,A.; Arranz,C.; Balaszczuk,A.M.
Acute nitric oxide synthase inhibition with N G-nitro-L-arginine methyl ester (L-NAME) on chronotropic and pressor responses was studied in anesthetized intact rats and rats submitted to partial and complete autonomic blockade. Blood pressure and heart rate were monitored intra-arterially. Intravenous L-NAME injection (7.5 mg/kg) elicited the same hypertensive response in intact rats and in rats with partial (ganglionic and parasympathetic blockade) and complete autonomic blockade (38 ± 3, 55 ± 6, 54 ± 5, 45 ± 5 mmHg, respectively; N = 9, P = NS). L-NAME-induced bradycardia at the time when blood pressure reached the peak plateau was similar in intact rats and in rats with partial autonomic blockade (43 ± 8, 38 ± 5, 46 ± 6 bpm, respectively; N = 9, P =...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Autonomic nervous system; Heart rate; Heart.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500015

Cissampelos sympodialis Eichl (Menispermaceae) leaf extract induces interleukin-10-dependent inhibition of Trypanosoma cruzi killing by macrophages
Provedor de dados: 56
Autores: Alexandre-Moreira,M.S.; Freire-de-Lima,C.G.; Trindade,M.N.; Castro-Faria-Neto,H.C.; Piuvezam,M.R.; Peçanha,L.M.T.
The aqueous fraction of the ethanolic extract (AFL) of Cissampelos sympodialis Eichl (Menispermaceae), popularly known as milona, has been shown to have both immunosuppressive and anti-inflammatory effects. In the present study we investigated the modulation of macrophage antimicrobicidal activity by in vitro treatment with the extract from C. sympodialis. Normal and thioglycolate-elicited mouse peritoneal macrophages were infected in vitro with the protozoan Trypanosoma cruzi DM28c clone. We observed that the AFL (used at doses ranging from 13 to 100 µg/ml) increased T. cruzi growth and induced a 75% reduction in nitric oxide production. This inhibition could be mediated by the stimulation of macrophage interleukin-10 (IL-10) secretion since the in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cissampelos sympodialis; Macrophage; Trypanosoma cruzi; Nitric oxide; Interleukin-10.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200006

Effects of NO/cGMP inhibitors in a rat model of anaphylactoid shock
Provedor de dados: 56
Autores: Albuquerque,A.A.S.; Ferreira,L.G.; Carvalho,M.T.M.; Capellini,V.K.; Evora,P.R.B.; Celotto,A.C.
Anaphylactic shock can be defined as an acute syndrome, and it is the most severe clinical manifestation of allergic diseases. Anaphylactoid reactions are similar to anaphylactic events but differ in the pathophysiological mechanism. Nitric oxide (NO) inhibitors during anaphylaxis suggest that NO might decrease the signs and symptoms of anaphylaxis but exacerbate associated vasodilation. Therefore, blocking the effects of NO on vascular smooth muscle by inhibiting the guanylate cyclase (GC) would be a reasonable strategy. This study aimed to investigate the effects of NO/cGMP pathway inhibitors methylene blue (MB), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and indigo carmine (IC) in shock induced by compound 48/80 (C48/80) in rats. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endothelial cells; Allergy; Rodent; Nitric oxide; Anaphylaxis.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300606

Nitric oxide regulates angiotensin-I converting enzyme under static conditions but not under shear stress
Provedor de dados: 56
Autores: Pertrini,C.M.; Miyakawa,A.A.; Laurindo,F.R.M.; Krieger,J.E.
Mechanical forces including pressure and shear stress play an important role in vascular homeostasis via the control of the production and release of a variety of vasoactive factors. An increase in vascular shear stress is accompanied by nitric oxide (NO) release and NO synthase activation. Previously, we have demonstrated that shear stress induces angiotensin-I converting enzyme (ACE) down-regulation in vivo and in vitro. In the present study, we determined whether NO participates in the shear stress-induced ACE suppression response. Rabbit aortic endothelial cells were evaluated using the NO synthase inhibitor L-NAME, and two NO donors, diethylamine NONOate (DEA/NO) and sodium nitroprusside (SNP). Under static conditions, incubation of endothelial cells...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Shear stress; Angiotensin-I converting enzyme; Nitric oxide; Endothelial cells.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900005

Pharmacological characterization of the relaxant effect induced by adrenomedullin in rat cavernosal smooth muscle
Provedor de dados: 56
Autores: Leite,L.N.; Gonzaga,N.A.; Tirapelli,D.P.C.; Tirapelli,L.F.; Tirapelli,C.R.
The aim of the present study was to determine the mechanisms underlying the relaxant effect of adrenomedullin (AM) in rat cavernosal smooth muscle (CSM) and the expression of AM system components in this tissue. Functional assays using standard muscle bath procedures were performed in CSM isolated from male Wistar rats. Protein and mRNA levels of pre-pro-AM, calcitonin receptor-like receptor (CRLR), and Subtypes 1, 2 and 3 of the receptor activity-modifying protein (RAMP) family were assessed by Western immunoblotting and quantitative real-time polymerase chain reaction, respectively. Nitrate and 6-keto-prostaglandin F1α (6-keto-PGF1α; a stable product of prostacyclin) levels were determined using commercially available kits. Protein and mRNA of AM, CRLR,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rat cavernosal smooth muscle; Adrenomedullin; Relaxation; Nitric oxide; Calcitonin receptor-like receptor.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000876

Neuroendocrine control of body fluid homeostasis
Provedor de dados: 56
Autores: McCann,S.M.; Gutkowska,J.; Antunes-Rodrigues,J.
Angiotensin II and atrial natriuretic peptide (ANP) play important and opposite roles in the control of water and salt intake, with angiotensin II promoting the intake of both and ANP inhibiting the intake of both. Following blood volume expansion, baroreceptor input to the brainstem induces the release of ANP within the hypothalamus that releases oxytocin (OT) that acts on its receptors in the heart to cause the release of ANP. ANP activates guanylyl cyclase that converts guanosine triphosphate into cyclic guanosine monophosphate (cGMP). cGMP activates protein kinase G that reduces heart rate and force of contraction, decreasing cardiac output. ANP acts similarly to induce vasodilation. The intrinsic OT system in the heart and vascular system augments the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oxytocin; Vasopressin; Atrial natriuretic peptide; Nitric oxide; Angiotensin II; Guanylyl cyclase.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200003

Protective effect of N-acetylcysteine against oxygen radical-mediated coronary artery injury
Provedor de dados: 56
Autores: Rodrigues,A.J.; Évora,P.R.B.; Schaff,H.V.
The present study investigated the protective effect of N-acetylcysteine (NAC) against oxygen radical-mediated coronary artery injury. Vascular contraction and relaxation were determined in canine coronary arteries immersed in Kreb's solution (95% O2-5% CO2), incubated or not with NAC (10 mM), and exposed to free radicals (FR) generated by xanthine oxidase (100 mU/ml) plus xanthine (0.1 mM). Rings not exposed to FR or NAC were used as controls. The arteries were contracted with 2.5 µM prostaglandin F2alpha. Subsequently, concentration-response curves for acetylcholine, calcium ionophore and sodium fluoride were obtained in the presence of 20 µM indomethacin. Concentration-response curves for bradykinin, calcium ionophore, sodium nitroprusside, and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coronary arteries; N-acetylcysteine; Endothelium; Oxidative stress; Nitric oxide.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800012

Mechanisms involved in the vasorelaxant effects produced by the acute application of amfepramone in vitro to rat aortic rings
Provedor de dados: 56
Autores: López-Canales,J.S.; Lozano-Cuenca,J.; Muãoz-Islas,E.; Aguilar-Carrasco,J.C.; López-Canales,O.A.; López-Mayorga,R.M.; Castillo-Henkel,E.F.; Valencia-Hernández,I.; Castillo-Henkel,C.
Amfepramone (diethylpropion) is an appetite-suppressant drug used for the treatment of overweight and obesity. It has been suggested that the systemic and central activity of amfepramone produces cardiovascular effects such as transient ischemic attacks and primary pulmonary hypertension. However, it is not known whether amfepramone produces immediate vascular effects when applied in vitro to rat aortic rings and, if so, what mechanisms may be involved. We analyzed the effect of amfepramone on phenylephrine-precontracted rat aortic rings with or without endothelium and the influence of inhibitors or blockers on this effect. Amfepramone produced a concentration-dependent vasorelaxation in phenylephrine-precontracted rat aortic rings that was not affected by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cardiovascular pharmacology; Amfepramone; Obesity; Rat aorta; Nitric oxide; Potassium channels.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600537

Ginkgo biloba leaf extract (EGb 761) enhances catalepsy induced by haloperidol and L-nitroarginine in mice
Provedor de dados: 56
Autores: Fontana,L.; Souza,A.S.; Del Bel,E.A.; Oliveira,R.M.W. de.
Ginkgo biloba extract EGb 761 has been reported to have therapeutic effects which have been attributed to anti-oxidant and free radical-scavenging activities, including a direct action on nitric oxide production. L G-nitro-arginine (L-NOARG), a nitric oxide synthase inhibitor, and haloperidol, a drug that blocks dopamine receptors, are both known to induce catalepsy in rodents. Nitric oxide has been shown to influence dopaminergic transmission in the striatum. The purpose of the present study was to evaluate the effect of the extract obtained from leaves of Ginkgo biloba tree EGb 761 on catalepsy induced by haloperidol or by L-NOARG. Albino Swiss mice (35-45 g, N = 8-12) received by gavage a single or repeated oral dose (twice a day for 4 days) of EGb 761...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Ginkgo biloba; Catalepsy; Herbal medicine; Neuroleptic interaction; Extrapyramidal system.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100012

Estrogen enhances vasoconstrictive remodeling after injury in male rabbits
Provedor de dados: 56
Autores: Francisco,Y.A.; Dantas,A.P.V.; Carvalho,M.H.C.; Laurindo,F.R.M.
The complete spectrum of estrogen vascular effects remains unclear. In particular, estrogen effects in the vascular response to profound injury in males have not been explored in detail. Therefore, we submitted 44 male New Zealand rabbits weighing 3.4 ± 0.6 kg to overdistention balloon injury of the right iliac artery. Rabbits were given 17ß-estradiol (5.45 µmol/day, sc) or vehicle for 7 days before and 14 days after injury, when the arteries were examined by post-mortem histomorphometry. Arteriographic caliber was assessed in vivo at baseline and before sacrifice. On day 14 after injury, in vivo arteriographic caliber (baseline = 2.44 ± 0.43 mm) was decreased by 23.1 ± 0.1% in controls and by 44.5 ± 0.1% in estrogen-treated rabbits (P &lt; 0.001). Neither...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Estrogen; Restenosis post-angioplasty; Nitric oxide; Endothelial function; Vascular remodeling.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000900006

Induction of cell-mediated immunity during early stages of infection with intracellular protozoa
Provedor de dados: 56
Autores: Gazzinelli,R.T.; Talvani,A.; Camargo,M.M.; Santiago,H.C.; Oliveira,M.A.P.; Vieira,L.Q.; Martins,G.A.; Aliberti,J.C.S.; Silva,J.S.
Toxoplasma gondii and Trypanosoma cruzi are intracellular parasites which, as part of their life cycle, induce a potent cell-mediated immunity (CMI) maintained by Th1 lymphocytes and IFN-&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;. In both cases, induction of a strong CMI is thought to protect the host against rapid parasite multiplication and consequent pathology and lethality during the acute phase of infection. However, the parasitic infection is not eliminated by the immune system and the vertebrate host serves as a parasite reservoir. In contrast, Leishmania sp, which is a slow growing parasite, appears to evade induction of CMI during early stages of infection as a strategy for surviving in a hostile environment (i.e., inside the macrophages which are...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leishmania; Toxoplasma gondii; Trypanosoma cruzi; Macrophages; Cytokines; Nitric oxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100012

Influence of eNOS gene polymorphism on cardiometabolic parameters in response to physical training in postmenopausal women
Provedor de dados: 56
Autores: Esposti,R.D.; Sponton,C.H.G.; Malagrino,P.A.; Carvalho,F.C.; Peres,E.; Puga,G.M.; Novais,I.P.; Albuquerque,D.M.; Rodovalho,C.; Bacci,M.; Zanesco,A.
The health-promoting effects of exercise training (ET) are related to nitric oxide (NO) production and/or its bioavailability. The objective of this study was to determine whether single nucleotide polymorphism of the endothelial NO synthase (eNOS) gene at positions -786T&gt;C, G894T (Glu298Asp) and at the variable number of tandem repeat (VNTR) Intron 4b/a would interfere with the cardiometabolic responses of postmenopausal women submitted to physical training. Forty-nine postmenopausal women were trained in sessions of 30-40 min, 3 days a week for 8 weeks. Genotypes, oxidative stress status and cardiometabolic parameters were then evaluated in a double-blind design. Both systolic and diastolic blood pressure values were significantly reduced after ET,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Exercise training; ENOS polymorphism; Oxidative stress; Nitric oxide; Women.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000900005

Cytotoxic activity of BCG-activated macrophages against L929 tumor cells is nitric oxide-dependent
Provedor de dados: 56
Autores: Nascimento,F.R.F.; Ribeiro-Dias,F.; Russo,M.
The tumoricidal activity of activated macrophages has been attributed largely to the release of tumor necrosis factor (TNF), or to the production of reactive oxygen or nitrogen intermediates. The L929 tumor cell line (a murine fibroblast-like cell) when treated with actinomycin D (ActD) has been used to measure TNF&lt;FONT FACE="Symbol"&gt;a&lt;/FONT&gt; cytotoxicity. In the present study, we determined the cytotoxic activity of BCG-activated peritoneal macrophages against ActD-untreated L929 tumor cells. Furthermore, we measured the production of hydrogen peroxide (H2O2), nitric oxide (NO) and TNF by macrophages cultured in the presence or absence of L929 cells. As expected, BCG-activated macrophages produced significant amounts of H2O2 (16.0 ± 3.0 µM),...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cytotoxicity; Macrophages; L929 tumor cells; Nitric oxide; TNF; Hydrogen peroxide.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200012

Gentamicin-induced preconditioning of proximal tubular LLC-PK1 cells stimulates nitric oxide production but not the synthesis of heat shock protein
Provedor de dados: 56
Autores: Pessoa,E.A.; Convento,M.B.; Silva,R.G.; Oliveira,A.S.; Borges,F.T.; Schor,N.
Nephrotoxicity is the main side effect of antibiotics such as gentamicin. Preconditioning has been reported to protect against injuries as ischemia/reperfusion. The objective of the present study was to determine the effect of preconditioning with gentamicin on LLC-PK1 cells. Preconditioning was induced in LLC-PK1 cells by 24-h exposure to 2.0 mM gentamicin (G/IU). After 4 or 15 days of preconditioning, cells were again exposed to gentamicin (2.0 mM) and compared to untreated control or G/IU cells. Necrosis and apoptosis were assessed by acridine orange and HOESCHT 33346. Nitric oxide (NO) and endothelin-1 were assessed by the Griess method and available kit. Heat shock proteins were analyzed by Western blotting. After 15 days of preconditioning, LLC-PK1...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pre-conditioning; Gentamicin; Nitric oxide; LLC-PK1 cells; Acute kidney injury; Apoptosis.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700005

Unconventional neurotransmitters, neurodegeneration and neuroprotection
Provedor de dados: 56
Autores: Leonelli,M.; Torrão,A.S.; Britto,L.R.G.
Neurotransmitters are also involved in functions other than conventional signal transfer between nerve cells, such as development, plasticity, neurodegeneration, and neuroprotection. For example, there is a considerable amount of data indicating developmental roles for the glutamatergic, cholinergic, dopaminergic, GABA-ergic, and ATP/adenosine systems. In this review, we discuss the existing literature on these "new" functions of neurotransmitters in relation to some unconventional neurotransmitters, such as the endocannabinoids and nitric oxide. Data indicating both transcriptional and post-transcriptional modulation of endocannabinoid and nitrinergic systems after neural lesions are discussed in relation to the non-conventional roles of these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Endocannabinoids; Cannabinoid receptors; Vanilloid receptors; Nitric oxide.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000100011

Participation of the NO/cGMP/K+ATP pathway in the antinociception induced by Walker tumor bearing in rats
Provedor de dados: 56
Autores: Barbosa,A.L.R.; Pinheiro,C.A.; Oliveira,G.J.; Torres,J.N.L.; Moraes,M.O.; Ribeiro,R.A.; Vale,M.L.; Souza,M.H.L.P.
Implantation of Walker 256 tumor decreases acute systemic inflammation in rats. Inflammatory hyperalgesia is one of the most important events of acute inflammation. The L-arginine/NO/cGMP/K+ATP pathway has been proposed as the mechanism of peripheral antinociception mediated by several drugs and physical exercise. The objective of this study was to investigate a possible involvement of the NO/cGMP/K+ATP pathway in antinociception induced in Walker 256 tumor-bearing male Wistar rats (180-220 g). The groups consisted of 5-6 animals. Mechanical inflammatory hypernociception was evaluated using an electronic version of the von Frey test. Walker tumor (4th and 7th day post-implantation) reduced prostaglandin E2- (PGE2, 400 ng/paw; 50 µL; intraplantar injection)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Walker 246 tumor-bearing rats; Hypernociception; Nitric oxide; Carrageenan.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600009

Role of nitric oxide of the median preoptic nucleus (MnPO) in the alterations of salivary flow, arterial pressure and heart rate induced by injection of pilocarpine into the MnPO and intraperitoneally
Provedor de dados: 56
Autores: Saad,Wilson A.; Guarda,I.F.M.S.; Camargo,L.A.A.; Santos,T.A.F.B.; Guarda,R.S.; Saad,Willian A.; Simões,S.; Antunes Rodrigues,J.
We investigated the effect of L-NAME, a nitric oxide (NO) inhibitor and sodium nitroprusside (SNP), an NO-donating agent, on pilocarpine-induced alterations in salivary flow, mean arterial blood pressure (MAP) and heart rate (HR) in rats. Male Holtzman rats (250-300 g) were implanted with a stainless steel cannula directly into the median preoptic nucleus (MnPO). Pilocarpine (10, 20, 40, 80, 160 µg) injected into the MnPO induced an increase in salivary secretion (P&lt;0.01). Pilocarpine (1, 2, 4, 8, 16 mg/kg) ip also increased salivary secretion (P&lt;0.01). Injection of L-NAME (40 µg) into the MnPO prior to pilocarpine (10, 20, 40, 80, 160 µg) injected into the MnPO or ip (1, 2, 4, 8, 16 mg/kg) increased salivary secretion (P&lt;0.01). SNP (30 µg)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pilocarpine; Nitric oxide; Central nervous system; Salivation; Arterial pressure; Heart rate.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700010

Correlation between total nitrite/nitrate concentrations and monoamine oxidase (types A and B) and semicarbazide-sensitive amine oxidase enzymatic activities in human mesenteric arteries from non-diabetic and type 2 diabetic patients
Provedor de dados: 56
Autores: Nunes,S.F.; Figueiredo,I.V.; Pereira,J.S.; Lopes,M.C.; Caramona,M.M.
The aim of this study was to determine the correlation between total nitrite/nitrate concentrations (NOx) and the kinetic parameters of monoamine oxidase enzymes (MAO-A and MAO-B) and semicarbazide-sensitive amine oxidase (SSAO) in human mesenteric arteries. Arteries were from non-diabetic and type 2 diabetic patients with sigmoid or rectum carcinoma for whom surgery was the first option and who were not exposed to neo-adjuvant therapy. Segments of human inferior mesenteric arteries from non-diabetic (61.1 ± 8.9 years old, 7 males and 5 females, N = 12) and type 2 diabetic patients (65.8 ± 6.2 years old, 8 males and 4 females, N = 12) were used to determine NOx concentrations and the kinetic parameters of MAO-A, MAO-B and SSAO by the Griess reaction and by...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Monoamine oxidase; Semicarbazide-sensitive amine oxidase; Mesenteric arteries; Type 2 diabetes.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000100004

Effects of L-arginine on the diaphragm muscle twitches elicited at different frequencies of nerve stimulation
Provedor de dados: 56
Autores: Queiroz,R.N.; Alves-Do-Prado,Wilson.
In rats, the nitric oxide (NO)-synthase pathway is present in skeletal muscle, vascular smooth muscle, and motor nerve terminals. Effects of NO were previously studied in rat neuromuscular preparations receiving low (0.2 Hz) or high (200 Hz) frequencies of stimulation. The latter frequency has always induced tetanic fade. However, in these previous studies we did not determine whether NO facilitates or impairs the neuromuscular transmission in preparations indirectly stimulated at frequencies which facilitate neuromuscular transmission. Thus, the present study was carried out to examine the effects of NO in rat neuromuscular preparations indirectly stimulated at 5 and 50 Hz. The amplitude of muscular contraction observed at the end (B) of a 10-s...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Skeletal muscle; Nitric oxide; L-arginine; Neuromuscular transmission; Tetanic fade; S-nitroso-N-acetylpenicillamine; Superoxide dismutase.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600020

Endogenous angiotensin II modulates nNOS expression in renovascular hypertension
Provedor de dados: 56
Autores: Pereira,T.M.C.; Balarini,C.M.; Silva,I.V.; Cabral,A.M.; Vasquez,E.C.; Meyrelles,S.S.
Nitric oxide (NO) influences renal blood flow mainly as a result of neuronal nitric oxide synthase (nNOS). Nevertheless, it is unclear how nNOS expression is modulated by endogenous angiotensin II, an inhibitor of NO function. We tested the hypothesis that the angiotensin II AT1 receptor and oxidative stress mediated by NADPH oxidase contribute to the modulation of renal nNOS expression in two-kidney, one-clip (2K1C) hypertensive rats. Experiments were performed on male Wistar rats (150 to 170 g body weight) divided into 2K1C (N = 19) and sham-operated (N = 19) groups. nNOS expression in kidneys of 2K1C hypertensive rats (N = 9) was compared by Western blotting to that of 2K1C rats treated with low doses of the AT1 antagonist losartan (10 mg·kg-1·day-1; N...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Losartan; Tempol; Renovascular hypertension.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700014

Acute but not chronic metabolic acidosis potentiates the acetylcholine-induced reduction in blood pressure: an endothelium-dependent effect
Provedor de dados: 56
Autores: Celotto,A.C.; Ferreira,L.G.; Capellini,V.K.; Albuquerque,A.A.S.; Rodrigues,A.J.; Evora,P.R.B.
Metabolic acidosis has profound effects on vascular tone. This study investigated the in vivo effects of acute metabolic acidosis (AMA) and chronic metabolic acidosis (CMA) on hemodynamic parameters and endothelial function. CMA was induced by ad libitum intake of 1% NH4Cl for 7 days, and AMA was induced by a 3-h infusion of 6 M NH4Cl (1 mL/kg, diluted 1:10). Phenylephrine (Phe) and acetylcholine (Ach) dose-response curves were performed by venous infusion with simultaneous venous and arterial blood pressure monitoring. Plasma nitrite/nitrate (NOx) was measured by chemiluminescence. The CMA group had a blood pH of 7.15±0.03, which was associated with reduced bicarbonate (13.8±0.98 mmol/L) and no change in the partial pressure of arterial carbon dioxide...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acid-base balance; Metabolic acidosis; Hemodynamic failure; Endothelium; Nitric oxide.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200604

Blockade of the action of nitric oxide in human septic shock increases systemic vascular resistance and has detrimental effects on pulmonary function after a short infusion of methylene blue
Provedor de dados: 56
Autores: Weingartner,R.; Oliveira,E.; Oliveira,E.S.; Sant'Anna,U.L.; Oliveira,R.P.; Azambuja,L.A.; Friedman,G.
To investigate the role of nitric oxide in human sepsis, ten patients with severe septic shock requiring vasoactive drug therapy and mechanical ventilation were enrolled in a prospective, open, non-randomized clinical trial to study the acute effects of methylene blue, an inhibitor of guanylate cyclase. Hemodynamic and metabolic variables were measured before and 20, 40, 60, and 120 min after the start of a 1-h intravenous infusion of 4 mg/kg of methylene blue. Methylene blue administration caused a progressive increase in mean arterial pressure (60 [55-70] to 70 [65-100] mmHg, median [25-75th percentiles]; P&amp;lt;0.05), systemic vascular resistance index (649 [479-1084] to 1066 [585-1356] dyne s-1 cm-5 m-2; P&amp;lt;0.05) and the left ventricular stroke...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vasodilation; Myocardial depression; Septic shock; Methylene blue; Nitric oxide; Guanylate cyclase.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200009

Role of nitric oxide in hypoxia-induced hyperventilation and hypothermia: participation of the locus coeruleus
Provedor de dados: 56
Autores: Fabris,G.; Anselmo-Franci,J.A.; Branco,L.G.S.
Hypoxia elicits hyperventilation and hypothermia, but the mechanisms involved are not well understood. The nitric oxide (NO) pathway is involved in hypoxia-induced hypothermia and hyperventilation, and works as a neuromodulator in the central nervous system, including the locus coeruleus (LC), which is a noradrenergic nucleus in the pons. The LC plays a role in a number of stress-induced responses, but its participation in the control of breathing and thermoregulation is unclear. Thus, in the present study, we tested the hypothesis that LC plays a role in the hypoxia-induced hypothermia and hyperventilation, and that NO is involved in these responses. Electrolytic lesions were performed bilaterally within the LC in awake unrestrained adult male Wistar rats...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Locus coeruleus; Hypoxia; Ventilation; Body temperature; Rat.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001100009

Chronic aerobic exercise associated to low-dose L-NAME improves contractility without changing calcium handling in rat cardiomyocytes
Provedor de dados: 56
Autores: Luchi,T.C.; Coelho,P.M.; Cordeiro,J.P.; Assis,A.L.E.M.; Nogueira,B.V.; Marques,V.B.; dos Santos,L.; Lima-Leopoldo,A.P.; Lunz,W.; Leopoldo,A.S.
Nitric oxide (NO) inhibition by high-dose NG-nitro-L-arginine methyl ester (L-NAME) is associated with several detrimental effects on the cardiovascular system. However, low-dose L-NAME increases NO synthesis, which in turn induces physiological cardiovascular benefits, probably by activating a protective negative feedback mechanism. Aerobic exercise, likewise, improves several cardiovascular functions in healthy hearts, but its effects are not known when chronically associated with low-dose L-NAME. Thus, we tested whether the association between low-dose L-NAME administration and chronic aerobic exercise promotes beneficial effects to the cardiovascular system, evaluating the cardiac remodeling process. Male Wistar rats were randomly assigned to control...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Aerobic exercise; Cardiac remodeling; Cardiomyocyte; Calcium.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300611

Interferon gamma is a key cytokine in lung phase immunity to schistosomes but what is its precise role?
Provedor de dados: 56
Autores: Wilson,R.A.
Vaccination of mice with radiation-attenuated cercariae of Schistosoma mansoni induces a high level of protection against challenge with normal larvae. The immune effector mechanism, which operates in the lungs, is a cell-mediated delayed-type hypersensitivity response and involves the formation of a tight focus of mononuclear cells around embolised larvae. CD4+ T cells with Th1 characteristics are a major component of the infiltrate. They secrete abundant interferon gamma (IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt;) upon antigen stimulation in vitro, whilst in vivo neutralisation of the cytokine results in 90% abrogation of immunity. IFN&lt;FONT FACE="Symbol"&gt;g&lt;/font&gt; can induce a large number of genes and an attempt has been made to identify...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Interferon gamma; Schistosoma mansoni; Lung phase immunity; Nitric oxide; Adhesion molecule.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100022

Lack of tolerance for the anti-dyskinetic effects of 7-nitroindazole, a neuronal nitric oxide synthase inhibitor, in rats
Provedor de dados: 56
Autores: Novaretti,N.; Padovan-Neto,F.E.; Tumas,V.; da-Silva,C.A.; Del Bel,E.A.
7-Nitroindazole (7-NI) inhibits neuronal nitric oxide synthase in vivo and reduces l-DOPA-induced dyskinesias in a rat model of parkinsonism. The aim of the present study was to determine if the anti-dyskinetic effect of 7-NI was subject to tolerance after repeated treatment and if this drug could interfere with the priming effect of l-DOPA. Adult male Wistar rats (200-250 g) with unilateral depletion of dopamine in the substantia nigra compacta were treated with l-DOPA (30 mg/kg) for 34 days. On the 1st day, 6 rats received ip saline and 6 received ip 7-NI (30 mg/kg) before l-DOPA. From the 2nd to the 26th day, all rats received l-DOPA daily and, from the 27th to the 34th day, they also received 7-NI before l-DOPA. Animals were evaluated before the drug...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nitric oxide; Nitric oxide synthase; Parkinson’s disease; L-DOPA; Dyskinesia; DOPA priming; 7-Nitroindazole.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001100006

The myth of nitric oxide in central cardiovascular control by the nucleus tractus solitarii
Provedor de dados: 56
Considerable evidence suggests that nitroxidergic mechanisms in the nucleus tractus solitarii (NTS) participate in cardiovascular reflex control. Much of that evidence, being based on responses to nitric oxide precursors or inhibitors of nitric oxide synthesis, has been indirect and circumstantial. We sought to directly determine cardiovascular responses to nitric oxide donors microinjected into the NTS and to determine if traditional receptor mechanisms might account for responses to certain of these donors in the central nervous system. Anesthetized adult Sprague Dawley rats that were instrumented for recording arterial pressure and heart rate were used in the physiological studies. Microinjection of nitric oxide itself into the NTS did not produce any...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Nitric oxide; Nucleus tractus solitarii; Cardiovascular control; Receptors; Nitrosothiol.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000400013

L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats
Provedor de dados: 60
Autores: Moraes,Natália Valadares de; Bergamaschi,Mateus Machado; Bianchi,Maria de Lourdes Pires; Bragheto,Juliana Bordinassi; Malfará,Wilson Roberto; Queiroz,Regina Helena Costa.
Dapsone use is frequently associated to hematological side effects such as methemoglobinemia and hemolytic anemia, which are related to N-hydroxylation mediated by the P450 enzyme system. The aim of the present study was to evaluate the influence of L-arginine supplementation, a precursor for the synthesis of nitric oxide, as single or multiple dose regimens on dapsone-induced methemoglobinemia. Male Wistar rats were treated with L-arginine at 5, 15, 30, 60 and 180 mg/kg doses (p.o., gavage) in single or multiple dose regimens 2 hours prior to dapsone administration (40 mg/kg, i.p.). The effect of the nitric oxide synthase inhibitor L-NAME was investigated by treatment with multiple doses of 30 mg/kg (p.o., gavage) 2 hours before dapsone administration....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dapsone; Methemoglobinemia; L-arginine; Nitric oxide; L-NAME.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100010

Nitric oxide releasing-dendrimers: an overview
Provedor de dados: 60
Autores: Roveda Júnior,Antonio Carlos; Franco,Douglas Wagner.
Platforms able to storage, release or scavenge NO in a controlled and specific manner is interesting for biological applications. Among the possible matrices for these purposes, dendrimers are excellent candidates for that. These molecules have been used as drug delivery systems and exhibit interesting properties, like the possibility to perform chemical modifications on dendrimers surface, the capacity of storage high concentrations of compounds of interest in the same molecule and the ability to improve the solubility and the biocompatibility of the compounds bonded to it. This review emphasizes the recent progress in the development and in the biological applications of different NO-releasing dendrimers and the nitric oxide release pathways in these...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dendrimer; Nitric oxide; Nitrosyl complexes; Diazeniumdiolate; Nitrosothiol.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000700002

Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants
Provedor de dados: 61
Autores: Sheokand,Sunita; Bhankar,Vinita; Sawhney,Veena.
Accumulation of salts in irrigated soils is one of the primary factors limiting yield in South Asia. We investigated whether exogenous nitric oxide (NO) supplementation as sodium nitroprusside has any ameliorating effect against NaCl induced oxidative damage in chickpea leaves. NaCl treatment (250 mM) alone and in combination with two concentrations of SNP (0.2 and 1 mM) were given to 50 days old chickpea plants for 2, 4 and 6 days. Salt stress adversely affected the relative membrane injury, lipid peroxidation levels, relative water content (RWC) and H2O2 content. The effect was time dependent. SNP treatments could ameliorate the toxic effect of short term salt stress of 2 days on relative membrane injury and partial amelioration was observed with 4 and 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; Chickpea; Lipid peroxidation; Nitric oxide; Salt stress.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000200002