Consulta: subjectFacets:"Nuclear DNA"
Registros recuperados: 9
Data/hora: 02/06/2024 02:45:31

Comparative cytogenetic studies of Bufo ictericus, B. paracnemis (Amphibia, Anura) and an intermediate form in sympatry
Provedor de dados: 74
Autores: Azevedo,MFC; Foresti,F.; Ramos,PRR; Jim,J.
Specimens of Bufo ictericus, Bufo paracnemis and a third type, considered an intermediate subgroup between these species, were cytogenetically studied by conventional Giemsa staining, C-banding and staining of the nucleolus organizer region (NOR). The nuclear DNA content and seroproteins were also analyzed to characterize these species, and verify the possibility of hybridization between them. Karyotypes and cytogenetic markers were essentially equal on the basis of the methods used. The DNA nuclear content found was 6.25 ± 0.30 pg/DNA in Bufo ictericus; 7.57 ± 0.40 pg/DNA in Bufo paracnemis and 7.04 ± 0.29 pg/DNA in the intermediate subgroup. Eletrophoresis of total blood serum in Bufo ictericus, Bufo paracnemis and the intermediate specimens revealed a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bufo; Hybridization; Cytogenetics; Nuclear DNA; Seroproteins.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300012

Nuclear DNA content determination in Characiformes fish (Teleostei, Ostariophysi) from the Neotropical region
Provedor de dados: 74
Autores: Carvalho,Margarida Lima; Oliveira,Claudio; Navarrete,Maria Clara; Froehlich,Otávio; Foresti,Fausto.
In the present study, nuclear DNA content was analyzed in 53 species of Characiformes fish from the Neotropical region. Diploid number ranged from 2n = 48 in Astyanax fasciatus, Gymnocorymbus ternetzi and Hyphessobrycon griemi to 2n = 102 in Potamorhina squamoralevis, with a modal number of 54 chromosomes. Nuclear DNA content ranged from 1.70 ± 0.04 pg of DNA per diploid nucleus in Acestrorhynchus pantaneiro to 3.94 ± 0.09 pg in Tetragonopterus chalceus. A general analysis showed a mean value of 2.9 pg of DNA per diploid nucleus. Very similar DNA content values were observed in the species of the family Cynodontidae which showed a variation of 3% between the two genera studied. Small variations were observed between populations of Gymnocorymbus ternetzi,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100010

Nuclear DNA content in 20 species of Siluriformes (Teleostei: Ostariophysi) from the Neotropical region
Provedor de dados: 74
Autores: Fenerich,Paulo César; Foresti,Fausto; Oliveira,Claudio.
In the present study, 20 species of Siluriformes fish were analyzed in order to determine their nuclear DNA content and compare these data with their diploid number. In addition, the extension and importance of the changes that occurred during the process of diversification in the group of Neotropical freshwater catfish were investigated. The only species studied of the family Doradidae, Rhinodoras d'orbignyi (2n = 58), presented 3.46 ± 0.13 pg of DNA. Among the species of the family Heptapteridae, the values of nuclear DNA content and the diploid numbers ranged from 1.13 ± 0.09 pg of DNA in Pimelodella sp. (2n = 46) to 2.38 ± 0.07 pg of DNA in Imparfinis mirini (2n = 58). The family Loricariidae showed the widest variation in diploid number and nuclear...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nuclear DNA; DNA content; Chromosome; Fish; Evolution.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300008

Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR
Provedor de dados: 74
Autores: Xia,Peng; Radpour,Ramin; Zachariah,Rebecca; Fan,Alex Xiu Cheng; Kohler,Corina; Hahn,Sinuhe; Holzgreve,Wolfgang; Zhong,Xiao Yan.
Quantification of circulating nucleic acids in plasma and serum could be used as a non-invasive diagnostic tool for monitoring a wide variety of diseases and conditions. We describe here a rapid, simple and accurate multiplex real-time PCR method for direct synchronized analysis of circulating cell-free (ccf) mitochondrial (mtDNA) and nuclear (nDNA) DNA in plasma and serum samples. The method is based on one-step multiplex real-time PCR using a FAM-labeled MGB probe and primers to amplify the mtDNA sequence of the ATP 8 gene, and a VIC-labeled MGB probe and primers to amplify the nDNA sequence of the glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) gene, in plasma and serum samples simultaneously. The efficiencies of the multiplex assays were measured in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Circulating cell-free DNA; Mitochondrial DNA; Nuclear DNA; Real-time PCR; Quantitative PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100003

Morphology and molecular phylogeny of prorocentrum consutum sp. nov. (dinophyceae), a new benthic dinoflagellate from south Brittany (northwestern France)
Provedor de dados: 5
Autores: Chomerat, Nicolas; Sellos, Daniel Y.; Zentz, Frederic; Nezan, Elisabeth.
A new marine benthic Prorocentrum species from sandy habitats of South Brittany (northwestern France), P. consutum sp. nov., is described using LM and SEM and molecular phylogenetic analyses. Cells have a subcircular to broadly ovoid shape and are plainly flattened. They are 57-61 lm long and 52-55 lm wide. A central pyrenoid is present, and the kidney-shaped nucleus is positioned in the posterior region. In right valve view, the periflagellar area is deeply excavated, and the left valve forms a prominent apical ridge. The periflagellar area consists of nine platelets, and a small narrow collar is present around the flagellar pore. The ornamentation of this new species is very peculiar and is characterized by a ring of round areolae located at the...
Tipo: Text
Palavras-chave: Taxonomy; SSU rDNA; SEM; Prorocentrum; Nuclear DNA; Molecular phylogeny; LSU rDNA; Dinoflagellates.
Ano: 2010
URL: http://archimer.ifremer.fr/doc/2010/publication-7449.pdf

Prorocentrum bimaculatum sp nov (Dinophyceae, prorocentrales), a new benthic dinoflagellate species from Kuwait (Arabian gulf)
Provedor de dados: 5
Autores: Chomerat, Nicolas; Saburova, Maria; Bilien, Gwenael; Al-yamani, Faiza.
A new benthic dinoflagellate species, Prorocentrum bimaculatum sp. nov., is studied from Kuwaits marine sediments, based on detailed morphological and molecular data. Cells are large, oblong oval in shape. They are 49.955.3 mu m long and 38.443.2 mu m wide. The ornamentation of this new species is peculiar, and characterized by smooth valves with large pores (0.320.50 mu m) scattered on their surface, except in two circular patches of similar to 15 mu m in diameter, devoid of ornamentation and located on both sides of the valve centers. The periflagellar area is widely triangular, located in a moderate excavation of the right valve, and comprises nine platelets. The intercalary band of P. bimaculatum is smooth. The molecular phylogenetic position of this...
Tipo: Text
Palavras-chave: Arabian Gulf; Benthic dinoflagellates; Kuwait; Molecular phylogeny; Morphology; Nuclear DNA; Prorocentrum; RDNA; SEM; Taxonomy.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00066/17756/15698.pdf

Mitochondrial and nuclear DNA phylogeography of  two cupped oysters Crassostrea gigas and Crassostrea angulata
Provedor de dados: 5
Autores: Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text
Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999
URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf

Genealogy of the nuclear β-fibrinogen intron 7 in Lissotriton boscai (Caudata, Salamandridae): concordance with mtDNA and implications for phylogeography and speciation
Provedor de dados: 16
Autores: Teixeira, J.; Martínez-Solano, I.; Buckley, D.; Tarroso, P.; García-París, M.; Ferrand, N.
The power of phylogeographic inference resides in its ability to integrate information from multiple sources in an iterative hypothesis-testing framework. In this paper, we build upon previous mtDNA-based hypotheses about the evolutionary history of the Iberian newt Lissotriton boscai using sequences of the highly variable nuclear β-fibrinogen intron 7. In addition to the nuclear sequences, we produced new mtDNA data across the species range to delineate contact zones and test the congruence between nuclear and mitochondrial datasets at the same level of spatial organization. Through a combination of phylogenetic, phylogeographic continuous diffusion, and genetic landscape modelling analyses, we infer the evolutionary history of the species. We found...
Tipo: Article / Letter to the editor
Palavras-chave: Β-fibint7; Genetic landscape shape; Iberian Peninsula; Mitochondrial DNA; Nuclear DNA; 42.82; 42.64.
Ano: 2015
URL: http://www.repository.naturalis.nl/record/578951

Morphological and genetic differentiation of Bufo toads: two cryptic species in Western Europe (Anura, Bufonidae)
Provedor de dados: 16
Autores: Arntzen, J.W.; McAtear, J.; Recuero, E.; Ziermann, J.M.; Ohler, A.; Alphen, J. van; Martínez-Solano, I.
The Common toad Bufo bufo sensu lato is a widespread, morphologically conserved taxon. Recent studies have uncovered deep genetic differentiation between population groups, highlighting the need to revise the current taxonomy of the group and recognize additional species. Here we investigate patterns of variation in molecular (a mitochondrial DNA restriction enzyme assay and sequence data for two nuclear DNA fragments totalling 979 bp) and 17 morphological variables in Northern France where two of these groups meet (B. bufo sensu stricto and B. spinosus), in order to delineate their contact zone and uncover characters that would allow discrimination of the two taxa. Mitochondrial DNA data show an abrupt transition from areas where B. bufo is present to...
Tipo: Article / Letter to the editor
Palavras-chave: Bufo bufo; Bufo spinosus; Common toad; Contact zone; France; Mitochondrial DNA; Morphometrics; Nuclear DNA; 42.82.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/475530