Consulta: subjectFacets:"Oocyte"
Registros recuperados: 32
Data/hora: 09/06/2024 13:55:26

Maternal transmission of mitochondrial diseases
Provedor de dados: 74
Autores: Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308

The Nme gene family in fish
Provedor de dados: 5
Autores: Desvignes, T.; Fostier, A.; Fauvel, Christian; Bobe, J.
The Nme gene family, also known as Nm23 or NDPK, is a very ancient gene family that can be found in all kingdoms of life. In the late eighties, a gene of the Nme family, NME1, was identified as the first metastatic suppressor gene, resulting in a major interest for this family. Due to the complexity of the family, the need for a unified and evolutionary-supported gene nomenclature was recently stressed by the scientific community. Based on a complete evolutionary history study of the gene family in metazoans and vertebrates, a unified nomenclature was recently proposed and accepted by gene nomenclature consortia. In addition to its well-documented role in tumor metastasis, members of the Nme family are also involved in a wide variety of cellular and...
Tipo: Text
Palavras-chave: Nm23; NDPK; RP2; Teleost; Oocyte; Ovary.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00133/24466/22504.pdf

X-Linked Retinitis Pigmentosa 2 Is a Novel Maternal-Effect Gene Required for Left-Right Asymmetry in Zebrafish
Provedor de dados: 5
Autores: Desvignes, Thomas; Nguyen, Thaovi; Chesnel, Franck; Bouleau, Aurelien; Fauvel, Christian; Bobe, Julien.
Retinitis pigmentosa 2 (RP2) gene is responsible for up to 20% of X-linked retinitis pigmentosa, a severe heterogeneous genetic disorder resulting in progressive retinal degeneration in humans. In vertebrates, several bodies of evidence have clearly established the role of Rp2 protein in cilia genesis and/or function. Unexpectedly, some observations in zebrafish have suggested the oocyte-predominant expression of the rp2 gene, a typical feature of maternal-effect genes. In the present study, we investigate the maternal inheritance of rp2 gene products in zebrafish eggs in order to address whether rp2 could be a novel maternal-effect gene required for normal development. Although both rp2 mRNA and corresponding protein are expressed during oogenesis, rp2...
Tipo: Text
Palavras-chave: Developmental biology; Egg developmental competence; Egg quality; Fish; Fish reproduction; Maternal-effect gene; Ndpk; Nme10; Oocyte; Oocyte-specific; Ovum; Teleost; Zygote.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00280/39099/37660.pdf

A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus
Provedor de dados: 5
Autores: De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment...
Tipo: Text
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf

Infection of the cockle Cerastoderma edule in the baie des veys (France) by the microsporidian parasite Steinhausia sp.
Provedor de dados: 5
Autores: Comtet, T; Garcia, Celine; Le Coguic, Yvette; Joly, Jean-pierre.
We report the occurrence of the microsporidian parasite Steinhausia sp. in the oocytes of the common cockle Cerastoderma edule in a natural population in France, where high mortalities occurred. Steinhausia sp. appeared primarily as sporocysts containing many small spores, and putative earlier developmental stages were also observed. Both its prevalence and infection intensity were low, and no host defence reaction was recognized, suggesting that Steinhausia sp. had no detrimental effect on C. edule. Its prevalence was higher in cockles lying on the sediment surface, but the significance of this observation could not be explained given the poor knowledge of the Steinhausia life cycle. The present data did not allow specific identification of the parasite,...
Tipo: Text
Palavras-chave: Cerastoderma edule; Steinhausia sp.; Microsporida; Oocyte; Baie des veys.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00000/10762/7586.pdf

Spawning energetics and otolith microchemistry provide insights into the stock structure of bonga shad Ethmalosa fimbriata
Provedor de dados: 5
Autores: Doering, Julian; Wagner, Carola; Tiedemann, Maik; Brehmer, Patrice; Ekau, Werner.
The gross energy content of spawning batches and the microchemistry of sagittal otoliths in individual female bonga shad Ethmalosa fimbriata were compared between contrasting sampling sites at the Senegalese southern coast and inside the hypersaline Sine Saloum Estuary. Results show that females spawning in the estuary's middle reaches invested almost three times more energy into reproduction (115 +/- 65 J g(-1) body mass) than their neritic counterparts (39 +/- 34 J g(-1) body mass). Also, female otolith levels of Ba:Ca, Sr:Ca and Zn:Ca either differed significantly between study sites or could be linked to heterogeneous environmental variables. A quadratic discriminant function analysis provided evidence of segregated spawning populations of E. fimbriata...
Tipo: Text
Palavras-chave: Clupeidae; Inverse estuary; Oocyte; Reproductive investment; Senegal; Stock discrimination; Spawning components.
Ano: 2019
URL: https://archimer.ifremer.fr/doc/00484/59567/62545.pdf

The nme gene family in zebrafish oogenesis and early development
Provedor de dados: 5
Autores: Desvignes, T.; Fauvel, Christian; Bobe, J.
After the recent report of the expression of several nme genes in the zebrafish gonads, the present study aimed at further analyzing the expression of nme genes in the ovary with special attention for the nme transcripts that are maternally inherited and could thus participate in the determination of oocyte developmental competence. The expression levels of all groups I and II nme genes were characterized by QPCR in a panel of zebrafish tissues. The nme genes exhibiting an ovarian expression were subsequently monitored throughout oogenesis and early development, and their expression sites characterized using in situ hybridization. Here, we show that nme2b1, nme3, nme4, and nme6 are highly expressed in the ovary and present in the zebrafish oocyte...
Tipo: Text
Palavras-chave: NDPK; Nm23; Oocyte; Maternal; Teleost; Vertebrate.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00051/16226/13778.pdf

Sensitivity of eastern oyster ( Crassostrea virginica) spermatozoa and oocytes to dispersed oil: Cellular responses and impacts on fertilization and embryogenesis
Provedor de dados: 5
Autores: Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P.
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were...
Tipo: Text
Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf

Morphological and Morphometric Aspects of Crassostrea rhizophorae (Guilding, 1828) Oocytes in Three Stages of the Gonadal Cycle
Provedor de dados: 77
Autores: Ferreira,M. A. P.; Paixão,L. F.; Alcântara-Neto,C. P.; Santos,S. S. D.; Rocha,R. M.
Crassostrea rhizophorae (Guilding, 1828) is one of the tropical species of oysters cultivated on the Brazilian shore. Despite its high commercial value, there is no information on the gonadal characteristics during the reproductive stages of this species. The objective of this work was to analyze the morphology and morphometry of Crassostrea rhizophorae oocytes and follicles in three stages of the gonadal cycle. Were analized histological sections of gonads considering that the oocytes presented visible nuclei and nucleoli, it was observed three gonadal cycle stages: early gametogenesis, growth and maturation. In the early gametogenesis stage, follicles and oocytes presented diameters of 180.29 (± 41.91) and 18.66 (± 6.85) µm, respectively, and presence of...
Tipo: Journal article
Palavras-chave: Crassostrea; Oocyte; Gonad; Morphology.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400023

Transfection of Gametes: A Method to Generate Transgenic Animals
Provedor de dados: 77
Autores: Esponda,Pedro.
The production of transgenic animals (TA) using transfected spermatozoa or eggs is commented. Different methods have been employed to introduce transgenes into the gametes of several vertebrates and invertebrates. Methods for the transfection of gametes have employed naked DNA, viral vectors, DNA/Liposome complexes, electroporation or high velocity microprojectiles. Spermatozoa and oocytes or eggs have showed a good transfection efficience (80% in some cases), and microscopical observations demonstrated that the transgenes appeared localized in the nucleus. Gametes have shown to be naturally protected against the entrance of foreign genes because some semino plasma or plasma membrane proteins block the entrance of foreign genes in spermatozoa. In the...
Tipo: Journal article
Palavras-chave: Eggs; Oocyte; Spermatozoa; Transfection; Transgenic animals.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000300017

Morphologic and Morphometric Analysis of the Ovary of Black Bengal Goat (Capra hircus)
Provedor de dados: 77
Autores: Haque,Ziaul; Haque,Azimul; Quasem,Md. Abul.
Ovary plays the vital role in the reproductive biology and biotechnology of female animals. With the aim to study the ovarian morphometry of Black Bengal goat, both right and left ovaries were collected from the slaughter houses of different Thanas under Mymensingh district. For each of the specimens, gross parameters such as weight, length and width were recorded. Then they were processed and stained with H &amp;amp; E for histomorphometry. Our study revealed that the right ovary (0.53±0.02 g) was heavier than the left (0.52±0.02 g). The length of the right ovary (1.26±0.04 cm) was lower than the left (1.28±0.02 cm) but the width of the right (0.94±0.02 cm) was greater than the left (0.90±0.03 cm). The diameter of ovarian follicles in the cortex was...
Tipo: Journal article
Palavras-chave: Morphometry; Ovarian follicles; Cortex; Medulla; Oocyte.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100002

Ovary Development and Maturation in Nezara viridula (L.) (Hemiptera: Pentatomidae)
Provedor de dados: 82
Autores: Fortes,P; Salvador,G; Cônsoli,FL.
Ovary development and maturation of Nezara viridula (L.) were evaluated by examining ovariole morphology and the alterations in the biochemical (protein synthesis related to reproduction) composition of the hemolymph. Quantitative and qualitative protein analyses were performed and ovary structural alterations for the pre-reproductive and reproductive stages were recorded. Total concentration of proteins in female hemolymph gradually increased until the end of the pre-mating stage, remaining unaltered thereafter. Proteins linked to reproduction (vitellogenins) appeared in the hemolymph 10 days after adult emergence and indicated the end of the pre-mating stage. After mating, total protein concentration in the hemolymph was lower compared to virgin females;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vitellogenesis; Reproduction; Oocyte.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000100013

Effects of various nutrients and renewal of medium on in vitro development of bovine oocytes matured and fertilized in vitro
Provedor de dados: 19
Autores: FUKUI, Yutaka; LEE, Eun Song; KANEZAWA, Yuka; 福井, 豊; 李, 殷松; 金澤, 由佳.
http://www.obihiro.ac.jp/~library/kenkyu.html
Palavras-chave: Amino acid; Glucose; In vitro development; Oocyte; Cattle.
Ano: 1995
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3790

Gonadal development and reproductive period of the characin Astyanax aff. bimaculatus (Characiformes: Characidae) in a tropical reservoir in southeastern Brazil
Provedor de dados: 103
Autores: Araújo,Francisco G.; Nascimento,Aparecida A.; Gomes,Iracema D.; Sales,Armando; Oliveira,Beatriz A. Chagas de.
ABSTRACT The gonadal development of the characin Astyanax aff. bimaculatus from Funil Reservoir, an impoundment in the middle reaches of the Paraiba do Sul River in southeastern Brazil was analysed through histological, histochemical and histometric techniques, and the spawning season was determined. Unbalanced sex ratio was found, with females outnumbering and reaching larger sizes than males. The following stages of oogenic cell development were identified for females: primary growth, pre-vitellogenic (cortical alveolar) and vitellogenic. In males, the following stages were determined: spermatogonia primary and secondary, spermatocyte primary and secondary, spermatid and spermatozoa. The spawning occurs most of the year, peaking in January-February, as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Histology; Oocyte; Spawning type; Spermatocyte.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702019000100326

Gonadal development and fecundity of the smooth weakfish Cynoscion leiarchus (Teleostei: Perciformes: Sciaenidae) in a tropical Brazilian bay
Provedor de dados: 103
Autores: Silva,José P. do Carmo; Costa,Marcus R. da; Gomes,Iracema D.; Araújo,Francisco G.
ABSTRACT Cynoscion leiarchus (Cuvier, 1830) is a coastal fish species that is widely distributed along the Western Atlantic coast, from Belize to Southern Brazil. In Brazil, C. leiarchus It is an important resource for commercial and artisanal fisheries. Our objective was to describe the oogenesis and spermatogenesis stages of the species and to estimate batch size and relative fecundity of a population at a tropical bay in southeastern Brazil. The examined specimens were obtained from artisanal catches that took place from July 2013 to June 2014. A total of 18 individuals (11 females and 7 males) were examined for histological analysis and 20 spawning-capable females were used to estimate fecundity. Five stages of oocyte development (primary growth,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Histology; Oocyte; Reproductive tactics; Sepetiba Bay.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702016000600500

Effects of the spermatozoa: oocyte ratio, water volume and water temperature on artificial fertilization and sperm activation of cascudo-preto
Provedor de dados: 96
Autores: Bombardelli,Robie Allan; Sanches,Eduardo Antônio; Baggio,Diego Mendes; Sykora,Renata Marino; Souza,Bruno Estevão de; Tessaro,Lucélia; Piana,Pitágoras Augusto.
The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×10³, 7×10(4), 7×10(5), 7×10(6) and 7×10(7) spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fish; Loricariidae; Oocyte; Reproduction; Semen.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000100001

Oocyte development in cattle: physiological and genetic aspects
Provedor de dados: 96
Autores: Britt,Jack H.
Oocytes in cattle are formed during embryogenesis and develop within individual follicles in the cortex of the ovary. Dormant primordial follicles become active and undergo progressive development at regular intervals commencing during the late fetal stage and continuing throughout adulthood. Once activated, follicles and oocytes in a cohort either grow to maturation and ovulation or undergo atresia, ultimately depleting the ovaries of germ cells. It takes an estimated 100 days for a follicle and its oocyte to reach the mature ovulatory stage. Within an individual, number of follicles in one ovary is similar to number in the other ovary; however, there are large differences among individuals in total number of follicles present in both ovaries. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Follicle; Ovary; Oocyte; Embryo; Fecundity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982008001300013

ผลของการเสริม Ethidium bromide หรือ CuSO4 ในปฏิกิริยา Loop-mediated isothermal amplification (LAMP) ต่อการเพิ่มความแม่นยำในการคัดเพศตัวอ่อนโคเนื้อ
Provedor de dados: 106
Autores: Saruttiwong Boonkong; Chainarong Navanukraw; Tossapol Moonmanee; Jiratti Thammasiri; Vilaivan Khanthusaeng; Aree Kraisoon.
The study was conducted to determine effect of EB or CS supplements in LAMP reaction on greater accuracy of sexing cattle embryos. Bovine ovaries were collected from the slaughterhouse, oocytes were aspirated from follicles then were evaluated morphology. Oocytes were cultured in TCM-199 at 38.5 ºC 24 h. For in vitro fertilization (IVF), oocytes were co-incubated with spermatozoa at 38.5 ºC 24 h. Fertilization rate was evaluated and then the embryos were in vitro cultured until morula stage. Then, embryo samples were frozen and stored at -196 ºC. Frozen embryos (n=35) were randomly extracted for total cellular DNA and amplified by PCR method. DNA samples were stored at -20 ºC. Sexing was determined by modification of LAMP method with either 1mM EB or 1M CS...
Tipo: PhysicalObject
Palavras-chave: Cattle; Bovine; Oocyte; LAMP reaction; Loop-mediated isothermal amplificatio; Ethidium bromide; EB; CuSO4; CS; DNA; Sexing embryo; In vitro fertilization; IVF; โคเนื้อ; โอโอไซต์; ลักษณะทางสัณฐานวิทยา; การคัดเพศตัวอ่อน; เทคนิคการคัดเพศ; ดีเอ็นเอ; ความแม่นยำ.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5813

ผลของวิธีการให้อาหารแบบขั้นบันไดในระยะวงรอบการเป็นสัดต่อการพัฒนาฟอลลิเคิลและคุณภาพของโอโอไซต์ของแม่แพะพื้นเมืองไทย
Provedor de dados: 106
Autores: Jaruk Nutthakornkul; Chainarong Navanukraw; Parwadee Pakdee; Chalong Wachirapakorn; Krittiya Lertchunhakiat.
Objective of this study was to determine the effect of stair-step feeding regimen during estrous cycle on follicular development and oocytes quality. Sixteen Thai-native non pregnant goats were used with the average age and body weight of 8 months and 17±0.6 kg, respectively. The does exhibited at least 2 normal estrous cycle and were housed in 1 m2 steel cages. Heat detection was daily recorded using a vasectomized buck. The does were randomly assigned into two groups: Control and Stair-step feeding group. In the control group, does were fed 100% of nutrients requirement for growth in puberty periods. Does consumed Ruzi grass hay, DM = 500 g/head/d and concentrate supplemented at 1% of body weight. Total feed intake was 670 g/head/d, (ME = 1.48 Mcal/kg/d,...
Tipo: Collection
Palavras-chave: Thai-native goats; Stair-step feeding regimen; Follicle; Oocyte; Estrous cycle; Feed intake; แพะ; พันธุ์พื้นเมืองไทย; ฟอลลิเคิล; โอโอไซต์; การให้อาหารแบบขั้นบันได; การกินอาหาร; ความต้องการโภชนะ; การเจริญเติบโต.
Ano: 2011
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5275

Use of different pressures for transvaginal follicular aspiration in mares
Provedor de dados: 45
Autores: Sá,M.A.F.; Paiva,S.O.; Dutra,G.A.; Barbosa,C.G.; Mello,M.R.B.; Jacob,J.C.F.
ABSTRACT The success of transvaginal follicular aspiration in mares can be influenced by several factors, such as vacuum pump pressure levels. The present study aimed to investigate the effect of different negative pressures (150, 280 and 400mmHg) of the vacuum pump on the oocyte recovery in the mares. The mares (n=10) were undergoing follicular aspiration using three different negative pressures for three consecutive estrous cycles as follows: G150 = 150mmHg (n = 10); G280 = 280mmHg (n = 10); G400 = 400mmHg (n = 10). Every estrous cycle, the group that the mare would participate was drawn, and each animal participated once in each group. Only preovulatory follicle was used, about 30 to 36 hours after application of hCG. To compare the results, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oocyte; Subfertility; Preovulatory follicle; HCG.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300529

Activation of bovine oocytes by strontium combined or not with an electric pulse
Provedor de dados: 45
Autores: Leal,C.L.V.; Méo,S.C.; Garcia,J.M.
O objetivo deste trabalho foi avaliar as taxas de ativação e de clivagem de oócitos bovinos tratados com estrôncio (10mm de SrCl2), após maturação in vitro por 27-28 horas. No experimento 1, os tratamentos foram: S4 - ativação pelo estrôncio por 4 horas; S12 - ativação pelo estrôncio por 12 horas; S30 - ativação pelo estrôncio por 30 horas; e P - ativação por pulso elétrico (3 pulsos de 1,0kv/cm). No experimento 2 os tratamentos foram: PS4 - ativação combinada pelo pulso elétrico e pelo estrôncio por 4 horas; S4P - ativação pelo estrôncio por 4 horas e pelo pulso elétrico; e PS30 - ativação pelo pulso elétrico e pelo estrôncio por 30 horas. No experimento 1, todos os tratamentos apresentaram taxas similares de ativação (83-90%; P&gt;0,05). Para clivagem, P...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cattle; Activation; Parthenogenesis; Oocyte; Strontium; Electric pulse.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300020

Laparoscopic ovum pick-up in spotted paca ( Cuniculus pacas )
Provedor de dados: 45
Autores: Barros,F.F.P.C.; Teixeira,P.P.M.; Uscategui,R.A.R.; Coutinho,L.N.; Brito,M.B.S.; Kawanami,A.E.; Almeida,V.T.; Mariano,R.S.G.; Nociti,R.P.; Machado,M.R.F.; Vicente,W.R.R.
ABSTRACT The aim of this work is study the laparoscopic ovum pick-up (LapOPU) technique in spotted paca, describing surgery details, complications and oocyte recovery rate. Nine healthy adult non-pregnant captive females were used, in a total of 39 procedures. When the surgical plane of anaesthesia was achieved, the females were positioned at 20º Trendelenburg. Three 6mm trocars were placed on right and left inguinal and hypogastric regions. Abdomen was inflated with CO2 and the intra-abdominal pressure was stablished in 10mmHg. Follicular punctures were performed moving the ovaries with atraumatic forceps. For punctures, an 18-gauge 3.5 inch long needle attached to a vacuum system with pressure not exceeding 65mmHg was used. Oocytes were recovered into...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Follicular aspiration; Laparoscopy; Oocyte; Rodent; Wild animals.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400858

Effect of Rolipram on in vitro maturation, gene expression and embryonic development in bovines
Provedor de dados: 45
Autores: Santana,B.B.; Sobral,G.G.; Gomes,E.T.; Batista,A.M.; Teixeira,L.P.R.; Tavares,K.C.S.; Bertolini,M.; Carneiro,G.F.
ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Rolipram; IVF; IVM; Oocyte.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501433

Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes
Provedor de dados: 45
Autores: Adona,P.R.; Leal,C.L.V.
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P&lt;0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P&lt;0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P&gt;0.05). After...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011

Reactive oxygen species in bovine embryo in vitro production
Provedor de dados: 47
Autores: Dalvit,G.C.; Cetica,P.D.; Pintos,L.N.; Beconi,M.T.
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO2: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed semen in the same culture conditions and embryo in vitro culture in IVC-mSOF at 90% N2: 5% CO2: 5% O2. ROS was determined in denuded...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Reactive oxygen species; Oxidative stress; Oocyte; Embryo; Bovine.
Ano: 2005
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200009

In vitro fertilization of the rock shrimp, Rhynchocinetes typus (Decapoda, Caridea): a review
Provedor de dados: 48
Autores: Dupré,Enrique M; Barros,Claudio.
This review compiles all the research done on gametes and fertilization in the rock shrimp, R. typus, and describes the sequence of events from the first gamete interaction to zygote formation and the first cleavage of the embryo, with light, fluorescence confocal and electron microscopes. Early studies showed that sperm from the vas deferens have a tack-shape with a "needle-like process" or rigid spike (RS) that extends from a semi-spherical body that contains the arms with chromatin and spines. Upon contact with seawater and by action of Na +, the arms and spines extend, producing an inverted umbrella form of the spermatozoa. The first sperm-oocyte interaction occurs between protein receptors type lectins of the sperm RS and oocyte chorion sperm ligands....
Tipo: Journal article
Palavras-chave: Spermatic ultrastructure; Oocyte; Sperm-egg interaction.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200003

Advantages and Applications of Cryopreservation in Fisheries Science
Provedor de dados: 52
Autores: Tsai,S.; Lin,C.
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014

Effect of Fibroblast Growth Factor 2 (FGF2) and Insulin Transferrin Selenium (ITS) on In Vitro Maturation, Fertilization and Embryo Development in Sheep
Provedor de dados: 52
Autores: Mondal,Sukanta; Mor,Avantika; Reddy,Ippala Janardana; Nandi,Sumanta; Parameswaragupta,PS.
The present study evaluated the effect of fibroblast growth factor 2 (FGF2) and insulin-transferrin-selenium (ITS) to the in vitro maturation and embryo culture media on ovine oocyte maturation, cleavage and embryo development. Oocytes having more than five layers of unexpanded cumulus cells and granular homogenous ooplasm were cultured into 50 μL droplets of eight different culture systems: (i) TCM-199 (Tissue Culture Medium-199); (ii) TCM-199+10 ng/mL FGF2; (iii) TCM-199+20 ng/mL FGF2; (iv) TCM-199+30 ng/mL FGF2; (v) TCM-199+10 ng/mL ITS; (vi) TCM-199+20 ng/mL ITS; (vii) TCM-199+30 ng/mL ITS and (viii) TCM-199+20 ng/mL ITS+20 ng/mL FGF2 in a CO2 incubator at 38.50C for 24 h. All the oocyte culture media were supplemented with 10% FBS, FSH (10 μg/mL) and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fibroblast growth factor 2; Insulin transferrin selenium; Oocyte; Embryo; Sheep.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400521

Ovary maturatıon stages and histological investigation of ovary of the Zebrafish (Danio rerio)
Provedor de dados: 52
Autores: Koç (Yön),Nazan Deniz; Aytekin,Yener; Yüce,Rikap.
Microscopic ovary features of Zebrafish were analyzed. Samples were fixed in Bouin’s solution, mounted in parafin and cut into 6-7 µm-thick slices which were stained with Hematoxylin and Eosin (HE), methylene blue and using Periodic-acid Schiff method (PAS). The thin sections in ultramicrotome with the thickness of approximately 0,5 - 0,7 µm were examined by using Jeol electron microscope following contrasting with Uranyl Acetate-Lead Citrate. The zebrafish was an extremely dynamic organ in which follicles underwent asynchronous development. The oocytes of Zebrafish ovary were observed in various phases. The oocyte development of zebrafish was divided into four stages (primary growth, cortical alveolus, vitellogenic and mature oocyte). Oocyte diameters...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zebrafish; Gonad; Oocyte; Development.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300010

Goat ovarian follicles express different levels of mRNA for inhibin-ßA subunit and activin-A stimulates secondary follicle growth in vitro
Provedor de dados: 65
Autores: Leitão,Cintia Camurça Fernandes; Costa,José Jackson Nascimento; Saraiva,Márcia Viviane Alves; Araújo,Valdevane Rocha; Figueiredo,José Ricardo; Hurk,Robert van den; Silva,José Roberto Viana.
This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pre-antral follicles; Caprine; Culture; Oocyte; Antrum formation; FSH-R.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100018

Pre-incubation of porcine semen reduces the incidence of polyspermy on embryos derived from low quality oocytes
Provedor de dados: 65
Autores: Brogni,Cláudio Francisco; Ohlweiler,Lain Uriel; Klein,Norton; Mezzalira,Joana Claudia; Cristani,Jose; Mezzalira,Alceu.
ABSTRACT: The main cause of low efficiency of in vitro produced porcine embryos is the high polyspermic penetration rates at fertilization, which is aggravated in low quality oocytes. Experiment 1 evaluated the embryo development in high and low quality oocytes. Experiment 2 evaluated the embryo development and quality of low quality oocytes fertilized with sperm pre-incubated during 0h (control), 0.5h, 1h and 1.5h. Experiment 3 investigated fertilization and monospermic rates of the same groups of Experiment 2. Experiment 4 evaluated embryo development, cell density, fertilization and monospermic rates of high quality oocytes using semen pre incubated during the best time observed in the previous experiments. Cleavage and blastocyst rates were analyzed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pig IVF; Embryo IVP; Monospermy; Fertilization; Oocyte.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000601113

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes
Provedor de dados: 65
Autores: Rosa,Paulo Roberto Antunes da; Bohrer,Rodrigo Camponogara; Ludke,Charles Alencar; Cesaro,Matheus Pedroti De; Pereira,Gabriel Ribas; Mondadori,Rafael Gianela; Antoniazzi,Alfredo Quites; Gonçalves,Paulo Bayard Dias.
The objective of this study was to investigate the mRNA expression and protein localization of Grb10 gene in bovine cumulus-oocyte complexes (COCs) from different follicle sizes. Firstly, it was investigated the mRNA expression to correlate with maturation rates. COCs from follicles at 1-3, 4-6, 6-8 and &amp;gt;8mm were used to evaluate Grb10 gene expression by qRT-PCR assay and nuclear maturation rates. It was observed that more competent oocytes (from follicles at 6-8 and &amp;gt;8mm; P&amp;gt;0.05), had lower Grb10 mRNA expression levels when compared to the oocytes from follicles at 1-3 and 4-6mm (P&amp;gt;0.05). After it was performed an immunofluorescence analysis in COCs from different follicle sizes (1-3, 4-6, 6-8 and &amp;gt;8mm) to investigate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: : mRNA; Protein; Maturation; Oocyte; Grb10.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500898